ADGRB1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADGRB1
Entrez Gene ID	524070
Full Name	adhesion G protein-coupled receptor B1
Synonyms	BAI1
General protein information	Preferred Names adhesion G protein-coupled receptor B1 Names brain-specific angiogenesis inhibitor 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015474338.1	XP_015329824.1	brain-specific angiogenesis inhibitor 1 isoform X1
XM_010811854.3	XP_010810156.1	adhesion G protein-coupled receptor B1 isoform X2
NM_001191541.1	NP_001178470.1	brain-specific angiogenesis inhibitor 1 precursor
XM_025001587.1	XP_024857355.1	adhesion G protein-coupled receptor B1 isoform X1
XM_010811854.2	XP_010810156.1	brain-specific angiogenesis inhibitor 1 isoform X1
XM_025001586.1	XP_024857354.1	adhesion G protein-coupled receptor B1 isoform X1
XM_025001588.1	XP_024857356.1	adhesion G protein-coupled receptor B1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	BAI1	NP_001693.2
Bos taurus (cattle)	BAI1	NP_001178470.1
Rattus norvegicus (Norway rat)	Bai1	NP_001164068.2
	BAI1	XP_002808536.1
Mus musculus (house mouse)	Bai1	NP_778156.2
Xenopus tropicalis (tropical clawed frog)	bai1	XP_002934514.2
Canis lupus familiaris (dog)	BAI1	XP_005628359.1
Gallus gallus (chicken)	BAI1	XP_004940131.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ADGRB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa222310	XM_025001586.1 Latest version!	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222310	XM_025001587.1 Latest version!	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222311	XM_010811854.3 Latest version!	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222312	XM_025001588.1 Latest version!	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa41030	NM_001191541.1 Latest version!	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa50811	XM_010811854.2 Latest version!	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa50811	XM_015474338.1 Latest version!	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADGRB1 ( XM_025001587.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857355.1 Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa222310
Clone ID Related Accession (Same CDS sequence)	XM_025001587.1 , XM_025001586.1
Accession Version	XM_025001587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adhesion G protein-coupled receptor B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCCGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGGTACAGC ACCATGAACT ACTGCTGGCT CTCCCTGGAG 
GGGGGACTGC TGTACGCCTT CGTGGGACCG GCCGCGGCCG TCGTGCTGGT GAACATGGTC 
ATCGGGATCC TGGTGTTCAA CAAGCTCGTG TCCAAGGACG GCATCACGGA CAAGAAGCTG 
AAGGAGCGGG CAGGGGCCTC GCTGTGGAGC TCCTGCGTGG TGCTGCCGCT GCTGGCGCTG 
ACCTGGATGT CTGCTGTGCT CGCCGTCACC GACCGCAGGT CCGCCCTCTT CCAGATCCTC 
TTTGCTGTCT TCGACTCGCT GGAGGGCTTC GTCATCGTGA TGGTGCACTG CATCCTACGG 
AGAGAGGTCC AGGACGCTGT GAAGTGCCGT GTGGTGGACC GCCAGGAGGA GGGCAACGGG 
GACTCAGGGG GCTCCTTCCA GAACGGCCAC GCCCAGCTCA TGACCGACTT TGAGAAGGAC 
GTGGATCTGG CCTGTAGATC AGGTGAGCGC CTGACAGTGC TGAACAAGGA CATTGCAGCT 
TGCCGCACGG CCACCATCAC AGGCACGCTC AAACGGCCAT CGCAGCCGGA GGAGGAGAAG 
CTGAAGCTGG CCCATGCCAA GGCGCCCTCC AACTTCAACA GCCTGCCGGC CAATGTATCC 
AAGCTGCACC TGCACGGCTC GCCCCGCTGC CCCGGCGGGC CCCTGCCCGA CTTCCCCAAC 
CACTCCCTGA CCCTCAAGAA AGACAGGGCG CCCAAGTCGT CGTTCGTTGG CGACGGGGAC 
ATCTTCAAGA AGCTGGACTC GGAGCTGAGC CGGGCCCAGG AGAAAGCCCT GGACACAAGC 
TACGTTATCC TGCCCACGGC CACGGCCACG CTGCGGCCCA AGCCCCCGGA GGAGCCCAAG 
TACAGCATCC ACATTGACCA GATGCCCCAG ACCCGCCTCA TCCACCTCAG CATGGCGCCT 
GACGCGGGGC TCCCGGCCCG CAGCCCGCCC TCCCGCCAGC CCCCCGGCAG CGGGCCCCCC 
GAGCCGCCCC CCACCCAGCC CCCGCCGCCT CCGCCCCCGC CACCCCCACC GCCCCAGCAG 
CCCCTGCCCC CACCGCCCCC CATGGAGCCA GCACCCCCCA GCCTGGGGGA GCCCGGGGAG 
CCTGCTGCCC ACCAGGGGCC CAGTACGGGG CCCGGGACAA AGACAGAGAA TGTCTCCACG 
CTGTCCGTGA GCTCCCTGGA GCGGCGGAAA TCACGGTATG CGGAACTGGA CTTTGAGAAA 
ATCATGCACA CACGGAAACG GCACCAGGAC ATGTTCCAGG ACCTGAACCG GAAGTTACAG 
CACGCAGAGA AGGACAAGGA TGTGCTGGGC CCAGACAGCA AGCCAGAAAA GCAGCAGACG 
CCCAACAAGC GGCCCTGGGA GAGCCTCCGG AAAGCGCACG GGCCGCCCGC CTGGGTGAAG 
AAGGAGCTGG AGCCGCTCCC ACCGTCCCCA CTGGAGCTGC GGAGTGTGGA GTGGGAGAAG 
TCGGGCGCCA CCATCCCGCT GGTGGGCCAG GACATCATCG ACCTCCAGAC CGAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857355.1
CDS	271..5010
Translation	MRGQAAGPGALWILGLLLLLGRQVGTAAGTAAGPGAEPCATLVQGKFFGYFSAAAVFPANASRCSWTLRNPDPRRYTLYMKVAKAPACSGPARVRTYQFDSFLESTRTYLGVESFDEVLRLCDPSAPLAFLQASKQFLQMQRLRPPQDNTLRTRDGPPGPSDDFSVEYLVVGNRNPSRAACQMLCRWLDACLAGSRSSHPCGIMQTPCACLGGDTSDSASGSLAPRGDVCLRDGVAGGPENCLTSLTQDRGGDGTPGGWKLWSLWGECTRDCGGGLQTRTRTCLPAPGIQGGGCEGVLEEGRLCNRKACGPAGRTNSRSQSLRSTDARRREELGDDLQQFGFPAPQTGDPAAEEWSPWSVCSSTCGEGWQTRTRFCVSTSYSTQCSGPLREQRLCNNSAVCPVHGAWDEWSPWSLCSSTCGRGFRDRTRTCRPPQFGGNPCEGPEKQTKFCNIALCPVDGNWNEWSSWSTCSASCSQGRQQRTRECNGPSYGGAECQGHWVETRDCFLQQCPVDGKWQAWASWGSCSVTCGGGTQRRERACLGPFFGGVACQGPQDEYRQCGAQRCPEPHEICDEDNFGAVVWKETPAGEVAAVRCPRNATGLILRRCELDEEGIAYWEPPTYIRCVSIDYRNIQMMTREHLAKAQRGLPGEGVSEVIQTLVEISQDGTSYSGDLLSTIDVLRNMTEIFRRAYYSPTPGDVQNFVQIISNLLAEENRDKWEEAQLMGPNAKELFRLVEDFVDVIGFRMKDLRDAYQVTDNLVLSIHKLPASGATDISFPMKGWRATGDWAKVPEDRVTVSKSVFSTGLAEADDSSVFVVGTVLYRNLGSFLALQRNTTVLNSKVISVTVKPPPRSLLTPLEIEFAHMYNGTTNQTCILWDETDPPSSPAPPQLGPWSWRGCRTVPLDALRTRCLCDRLSTFAILAQLSADASMEKVATPSVTLIVGCGVSSLTLLMLIIIYVSVWRYIRSERSVILINFCLSIISSNALILIGQTQTRNKAVCTLVAAFLHFFFLSSFCWVLTEAWQSYMAVTGRLRNRLVRKRFLCLGWGLPALVVAISVGFTKARGYSTMNYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSKDGITDKKLKERAGASLWSSCVVLPLLALTWMSAVLAVTDRRSALFQILFAVFDSLEGFVIVMVHCILRREVQDAVKCRVVDRQEEGNGDSGGSFQNGHAQLMTDFEKDVDLACRSGERLTVLNKDIAACRTATITGTLKRPSQPEEEKLKLAHAKAPSNFNSLPANVSKLHLHGSPRCPGGPLPDFPNHSLTLKKDRAPKSSFVGDGDIFKKLDSELSRAQEKALDTSYVILPTATATLRPKPPEEPKYSIHIDQMPQTRLIHLSMAPDAGLPARSPPSRQPPGSGPPEPPPTQPPPPPPPPPPPPQQPLPPPPPMEPAPPSLGEPGEPAAHQGPSTGPGTKTENVSTLSVSSLERRKSRYAELDFEKIMHTRKRHQDMFQDLNRKLQHAEKDKDVLGPDSKPEKQQTPNKRPWESLRKAHGPPAWVKKELEPLPPSPLELRSVEWEKSGATIPLVGQDIIDLQTEV

Target ORF information:

RefSeq Version	XM_025001587.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001587.1

ORF Insert Sequence:

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCCGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGGTACAGC ACCATGAACT ACTGCTGGCT CTCCCTGGAG 
GGGGGACTGC TGTACGCCTT CGTGGGACCG GCCGCGGCCG TCGTGCTGGT GAACATGGTC 
ATCGGGATCC TGGTGTTCAA CAAGCTCGTG TCCAAGGACG GCATCACGGA CAAGAAGCTG 
AAGGAGCGGG CAGGGGCCTC GCTGTGGAGC TCCTGCGTGG TGCTGCCGCT GCTGGCGCTG 
ACCTGGATGT CTGCTGTGCT CGCCGTCACC GACCGCAGGT CCGCCCTCTT CCAGATCCTC 
TTTGCTGTCT TCGACTCGCT GGAGGGCTTC GTCATCGTGA TGGTGCACTG CATCCTACGG 
AGAGAGGTCC AGGACGCTGT GAAGTGCCGT GTGGTGGACC GCCAGGAGGA GGGCAACGGG 
GACTCAGGGG GCTCCTTCCA GAACGGCCAC GCCCAGCTCA TGACCGACTT TGAGAAGGAC 
GTGGATCTGG CCTGTAGATC AGGTGAGCGC CTGACAGTGC TGAACAAGGA CATTGCAGCT 
TGCCGCACGG CCACCATCAC AGGCACGCTC AAACGGCCAT CGCAGCCGGA GGAGGAGAAG 
CTGAAGCTGG CCCATGCCAA GGCGCCCTCC AACTTCAACA GCCTGCCGGC CAATGTATCC 
AAGCTGCACC TGCACGGCTC GCCCCGCTGC CCCGGCGGGC CCCTGCCCGA CTTCCCCAAC 
CACTCCCTGA CCCTCAAGAA AGACAGGGCG CCCAAGTCGT CGTTCGTTGG CGACGGGGAC 
ATCTTCAAGA AGCTGGACTC GGAGCTGAGC CGGGCCCAGG AGAAAGCCCT GGACACAAGC 
TACGTTATCC TGCCCACGGC CACGGCCACG CTGCGGCCCA AGCCCCCGGA GGAGCCCAAG 
TACAGCATCC ACATTGACCA GATGCCCCAG ACCCGCCTCA TCCACCTCAG CATGGCGCCT 
GACGCGGGGC TCCCGGCCCG CAGCCCGCCC TCCCGCCAGC CCCCCGGCAG CGGGCCCCCC 
GAGCCGCCCC CCACCCAGCC CCCGCCGCCT CCGCCCCCGC CACCCCCACC GCCCCAGCAG 
CCCCTGCCCC CACCGCCCCC CATGGAGCCA GCACCCCCCA GCCTGGGGGA GCCCGGGGAG 
CCTGCTGCCC ACCAGGGGCC CAGTACGGGG CCCGGGACAA AGACAGAGAA TGTCTCCACG 
CTGTCCGTGA GCTCCCTGGA GCGGCGGAAA TCACGGTATG CGGAACTGGA CTTTGAGAAA 
ATCATGCACA CACGGAAACG GCACCAGGAC ATGTTCCAGG ACCTGAACCG GAAGTTACAG 
CACGCAGAGA AGGACAAGGA TGTGCTGGGC CCAGACAGCA AGCCAGAAAA GCAGCAGACG 
CCCAACAAGC GGCCCTGGGA GAGCCTCCGG AAAGCGCACG GGCCGCCCGC CTGGGTGAAG 
AAGGAGCTGG AGCCGCTCCC ACCGTCCCCA CTGGAGCTGC GGAGTGTGGA GTGGGAGAAG 
TCGGGCGCCA CCATCCCGCT GGTGGGCCAG GACATCATCG ACCTCCAGAC CGAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB1 ( XM_025001586.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857354.1 Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa222310
Clone ID Related Accession (Same CDS sequence)	XM_025001587.1 , XM_025001586.1
Accession Version	XM_025001586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adhesion G protein-coupled receptor B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCCGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGGTACAGC ACCATGAACT ACTGCTGGCT CTCCCTGGAG 
GGGGGACTGC TGTACGCCTT CGTGGGACCG GCCGCGGCCG TCGTGCTGGT GAACATGGTC 
ATCGGGATCC TGGTGTTCAA CAAGCTCGTG TCCAAGGACG GCATCACGGA CAAGAAGCTG 
AAGGAGCGGG CAGGGGCCTC GCTGTGGAGC TCCTGCGTGG TGCTGCCGCT GCTGGCGCTG 
ACCTGGATGT CTGCTGTGCT CGCCGTCACC GACCGCAGGT CCGCCCTCTT CCAGATCCTC 
TTTGCTGTCT TCGACTCGCT GGAGGGCTTC GTCATCGTGA TGGTGCACTG CATCCTACGG 
AGAGAGGTCC AGGACGCTGT GAAGTGCCGT GTGGTGGACC GCCAGGAGGA GGGCAACGGG 
GACTCAGGGG GCTCCTTCCA GAACGGCCAC GCCCAGCTCA TGACCGACTT TGAGAAGGAC 
GTGGATCTGG CCTGTAGATC AGGTGAGCGC CTGACAGTGC TGAACAAGGA CATTGCAGCT 
TGCCGCACGG CCACCATCAC AGGCACGCTC AAACGGCCAT CGCAGCCGGA GGAGGAGAAG 
CTGAAGCTGG CCCATGCCAA GGCGCCCTCC AACTTCAACA GCCTGCCGGC CAATGTATCC 
AAGCTGCACC TGCACGGCTC GCCCCGCTGC CCCGGCGGGC CCCTGCCCGA CTTCCCCAAC 
CACTCCCTGA CCCTCAAGAA AGACAGGGCG CCCAAGTCGT CGTTCGTTGG CGACGGGGAC 
ATCTTCAAGA AGCTGGACTC GGAGCTGAGC CGGGCCCAGG AGAAAGCCCT GGACACAAGC 
TACGTTATCC TGCCCACGGC CACGGCCACG CTGCGGCCCA AGCCCCCGGA GGAGCCCAAG 
TACAGCATCC ACATTGACCA GATGCCCCAG ACCCGCCTCA TCCACCTCAG CATGGCGCCT 
GACGCGGGGC TCCCGGCCCG CAGCCCGCCC TCCCGCCAGC CCCCCGGCAG CGGGCCCCCC 
GAGCCGCCCC CCACCCAGCC CCCGCCGCCT CCGCCCCCGC CACCCCCACC GCCCCAGCAG 
CCCCTGCCCC CACCGCCCCC CATGGAGCCA GCACCCCCCA GCCTGGGGGA GCCCGGGGAG 
CCTGCTGCCC ACCAGGGGCC CAGTACGGGG CCCGGGACAA AGACAGAGAA TGTCTCCACG 
CTGTCCGTGA GCTCCCTGGA GCGGCGGAAA TCACGGTATG CGGAACTGGA CTTTGAGAAA 
ATCATGCACA CACGGAAACG GCACCAGGAC ATGTTCCAGG ACCTGAACCG GAAGTTACAG 
CACGCAGAGA AGGACAAGGA TGTGCTGGGC CCAGACAGCA AGCCAGAAAA GCAGCAGACG 
CCCAACAAGC GGCCCTGGGA GAGCCTCCGG AAAGCGCACG GGCCGCCCGC CTGGGTGAAG 
AAGGAGCTGG AGCCGCTCCC ACCGTCCCCA CTGGAGCTGC GGAGTGTGGA GTGGGAGAAG 
TCGGGCGCCA CCATCCCGCT GGTGGGCCAG GACATCATCG ACCTCCAGAC CGAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857354.1
CDS	343..5082
Translation	MRGQAAGPGALWILGLLLLLGRQVGTAAGTAAGPGAEPCATLVQGKFFGYFSAAAVFPANASRCSWTLRNPDPRRYTLYMKVAKAPACSGPARVRTYQFDSFLESTRTYLGVESFDEVLRLCDPSAPLAFLQASKQFLQMQRLRPPQDNTLRTRDGPPGPSDDFSVEYLVVGNRNPSRAACQMLCRWLDACLAGSRSSHPCGIMQTPCACLGGDTSDSASGSLAPRGDVCLRDGVAGGPENCLTSLTQDRGGDGTPGGWKLWSLWGECTRDCGGGLQTRTRTCLPAPGIQGGGCEGVLEEGRLCNRKACGPAGRTNSRSQSLRSTDARRREELGDDLQQFGFPAPQTGDPAAEEWSPWSVCSSTCGEGWQTRTRFCVSTSYSTQCSGPLREQRLCNNSAVCPVHGAWDEWSPWSLCSSTCGRGFRDRTRTCRPPQFGGNPCEGPEKQTKFCNIALCPVDGNWNEWSSWSTCSASCSQGRQQRTRECNGPSYGGAECQGHWVETRDCFLQQCPVDGKWQAWASWGSCSVTCGGGTQRRERACLGPFFGGVACQGPQDEYRQCGAQRCPEPHEICDEDNFGAVVWKETPAGEVAAVRCPRNATGLILRRCELDEEGIAYWEPPTYIRCVSIDYRNIQMMTREHLAKAQRGLPGEGVSEVIQTLVEISQDGTSYSGDLLSTIDVLRNMTEIFRRAYYSPTPGDVQNFVQIISNLLAEENRDKWEEAQLMGPNAKELFRLVEDFVDVIGFRMKDLRDAYQVTDNLVLSIHKLPASGATDISFPMKGWRATGDWAKVPEDRVTVSKSVFSTGLAEADDSSVFVVGTVLYRNLGSFLALQRNTTVLNSKVISVTVKPPPRSLLTPLEIEFAHMYNGTTNQTCILWDETDPPSSPAPPQLGPWSWRGCRTVPLDALRTRCLCDRLSTFAILAQLSADASMEKVATPSVTLIVGCGVSSLTLLMLIIIYVSVWRYIRSERSVILINFCLSIISSNALILIGQTQTRNKAVCTLVAAFLHFFFLSSFCWVLTEAWQSYMAVTGRLRNRLVRKRFLCLGWGLPALVVAISVGFTKARGYSTMNYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSKDGITDKKLKERAGASLWSSCVVLPLLALTWMSAVLAVTDRRSALFQILFAVFDSLEGFVIVMVHCILRREVQDAVKCRVVDRQEEGNGDSGGSFQNGHAQLMTDFEKDVDLACRSGERLTVLNKDIAACRTATITGTLKRPSQPEEEKLKLAHAKAPSNFNSLPANVSKLHLHGSPRCPGGPLPDFPNHSLTLKKDRAPKSSFVGDGDIFKKLDSELSRAQEKALDTSYVILPTATATLRPKPPEEPKYSIHIDQMPQTRLIHLSMAPDAGLPARSPPSRQPPGSGPPEPPPTQPPPPPPPPPPPPQQPLPPPPPMEPAPPSLGEPGEPAAHQGPSTGPGTKTENVSTLSVSSLERRKSRYAELDFEKIMHTRKRHQDMFQDLNRKLQHAEKDKDVLGPDSKPEKQQTPNKRPWESLRKAHGPPAWVKKELEPLPPSPLELRSVEWEKSGATIPLVGQDIIDLQTEV

Target ORF information:

RefSeq Version	XM_025001586.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001586.1

ORF Insert Sequence:

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCCGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGGTACAGC ACCATGAACT ACTGCTGGCT CTCCCTGGAG 
GGGGGACTGC TGTACGCCTT CGTGGGACCG GCCGCGGCCG TCGTGCTGGT GAACATGGTC 
ATCGGGATCC TGGTGTTCAA CAAGCTCGTG TCCAAGGACG GCATCACGGA CAAGAAGCTG 
AAGGAGCGGG CAGGGGCCTC GCTGTGGAGC TCCTGCGTGG TGCTGCCGCT GCTGGCGCTG 
ACCTGGATGT CTGCTGTGCT CGCCGTCACC GACCGCAGGT CCGCCCTCTT CCAGATCCTC 
TTTGCTGTCT TCGACTCGCT GGAGGGCTTC GTCATCGTGA TGGTGCACTG CATCCTACGG 
AGAGAGGTCC AGGACGCTGT GAAGTGCCGT GTGGTGGACC GCCAGGAGGA GGGCAACGGG 
GACTCAGGGG GCTCCTTCCA GAACGGCCAC GCCCAGCTCA TGACCGACTT TGAGAAGGAC 
GTGGATCTGG CCTGTAGATC AGGTGAGCGC CTGACAGTGC TGAACAAGGA CATTGCAGCT 
TGCCGCACGG CCACCATCAC AGGCACGCTC AAACGGCCAT CGCAGCCGGA GGAGGAGAAG 
CTGAAGCTGG CCCATGCCAA GGCGCCCTCC AACTTCAACA GCCTGCCGGC CAATGTATCC 
AAGCTGCACC TGCACGGCTC GCCCCGCTGC CCCGGCGGGC CCCTGCCCGA CTTCCCCAAC 
CACTCCCTGA CCCTCAAGAA AGACAGGGCG CCCAAGTCGT CGTTCGTTGG CGACGGGGAC 
ATCTTCAAGA AGCTGGACTC GGAGCTGAGC CGGGCCCAGG AGAAAGCCCT GGACACAAGC 
TACGTTATCC TGCCCACGGC CACGGCCACG CTGCGGCCCA AGCCCCCGGA GGAGCCCAAG 
TACAGCATCC ACATTGACCA GATGCCCCAG ACCCGCCTCA TCCACCTCAG CATGGCGCCT 
GACGCGGGGC TCCCGGCCCG CAGCCCGCCC TCCCGCCAGC CCCCCGGCAG CGGGCCCCCC 
GAGCCGCCCC CCACCCAGCC CCCGCCGCCT CCGCCCCCGC CACCCCCACC GCCCCAGCAG 
CCCCTGCCCC CACCGCCCCC CATGGAGCCA GCACCCCCCA GCCTGGGGGA GCCCGGGGAG 
CCTGCTGCCC ACCAGGGGCC CAGTACGGGG CCCGGGACAA AGACAGAGAA TGTCTCCACG 
CTGTCCGTGA GCTCCCTGGA GCGGCGGAAA TCACGGTATG CGGAACTGGA CTTTGAGAAA 
ATCATGCACA CACGGAAACG GCACCAGGAC ATGTTCCAGG ACCTGAACCG GAAGTTACAG 
CACGCAGAGA AGGACAAGGA TGTGCTGGGC CCAGACAGCA AGCCAGAAAA GCAGCAGACG 
CCCAACAAGC GGCCCTGGGA GAGCCTCCGG AAAGCGCACG GGCCGCCCGC CTGGGTGAAG 
AAGGAGCTGG AGCCGCTCCC ACCGTCCCCA CTGGAGCTGC GGAGTGTGGA GTGGGAGAAG 
TCGGGCGCCA CCATCCCGCT GGTGGGCCAG GACATCATCG ACCTCCAGAC CGAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB1 ( XM_010811854.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810156.1 Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X3, mRNA.

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CloneID	OBa222311
Clone ID Related Accession (Same CDS sequence)	XM_010811854.3
Accession Version	XM_010811854.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adhesion G protein-coupled receptor B1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010811854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCCGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGGTACAGC ACCATGAACT ACTGCTGGCT CTCCCTGGAG 
GGGGGACTGC TGTACGCCTT CGTGGGACCG GCCGCGGCCG TCGTGCTGGT GAACATGGTC 
ATCGGGATCC TGGTGTTCAA CAAGCTCGTG TCCAAGGACG GCATCACGGA CAAGAAGCTG 
AAGGAGCGGG CAGGGGCCTC GCTGTGGAGC TCCTGCGTGG TGCTGCCGCT GCTGGCGCTG 
ACCTGGATGT CTGCTGTGCT CGCCGTCACC GACCGCAGGT CCGCCCTCTT CCAGATCCTC 
TTTGCTGTCT TCGACTCGCT GGAGGGCTTC GTCATCGTGA TGGTGCACTG CATCCTACGG 
AGAGAGGTCC AGGACGCTGT GAAGTGCCGT GTGGTGGACC GCCAGGAGGA GGGCAACGGG 
GACTCAGGGG GCTCCTTCCA GAACGGCCAC GCCCAGCTCA TGACCGACTT TGAGAAGGAC 
GTGGATCTGG CCTGTAGATC AGTGCTGAAC AAGGACATTG CAGCTTGCCG CACGGCCACC 
ATCACAGGCA CGCTCAAACG GCCATCGCAG CCGGAGGAGG AGAAGCTGAA GCTGGCCCAT 
GCCAAGGCGC CCTCCAACTT CAACAGCCTG CCGGCCAATG TATCCAAGCT GCACCTGCAC 
GGCTCGCCCC GCTGCCCCGG CGGGCCCCTG CCCGACTTCC CCAACCACTC CCTGACCCTC 
AAGAAAGACA GGGCGCCCAA GTCGTCGTTC GTTGGCGACG GGGACATCTT CAAGAAGCTG 
GACTCGGAGC TGAGCCGGGC CCAGGAGAAA GCCCTGGACA CAAGCTACGT TATCCTGCCC 
ACGGCCACGG CCACGCTGCG GCCCAAGCCC CCGGAGGAGC CCAAGTACAG CATCCACATT 
GACCAGATGC CCCAGACCCG CCTCATCCAC CTCAGCATGG CGCCTGACGC GGGGCTCCCG 
GCCCGCAGCC CGCCCTCCCG CCAGCCCCCC GGCAGCGGGC CCCCCGAGCC GCCCCCCACC 
CAGCCCCCGC CGCCTCCGCC CCCGCCACCC CCACCGCCCC AGCAGCCCCT GCCCCCACCG 
CCCCCCATGG AGCCAGCACC CCCCAGCCTG GGGGAGCCCG GGGAGCCTGC TGCCCACCAG 
GGGCCCAGTA CGGGGCCCGG GACAAAGACA GAGAATGTCT CCACGCTGTC CGTGAGCTCC 
CTGGAGCGGC GGAAATCACG GTATGCGGAA CTGGACTTTG AGAAAATCAT GCACACACGG 
AAACGGCACC AGGACATGTT CCAGGACCTG AACCGGAAGT TACAGCACGC AGAGAAGGAC 
AAGGATGTGC TGGGCCCAGA CAGCAAGCCA GAAAAGCAGC AGACGCCCAA CAAGCGGCCC 
TGGGAGAGCC TCCGGAAAGC GCACGGGCCG CCCGCCTGGG TGAAGAAGGA GCTGGAGCCG 
CTCCCACCGT CCCCACTGGA GCTGCGGAGT GTGGAGTGGG AGAAGTCGGG CGCCACCATC 
CCGCTGGTGG GCCAGGACAT CATCGACCTC CAGACCGAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810156.1
CDS	342..5066
Translation	MRGQAAGPGALWILGLLLLLGRQVGTAAGTAAGPGAEPCATLVQGKFFGYFSAAAVFPANASRCSWTLRNPDPRRYTLYMKVAKAPACSGPARVRTYQFDSFLESTRTYLGVESFDEVLRLCDPSAPLAFLQASKQFLQMQRLRPPQDNTLRTRDGPPGPSDDFSVEYLVVGNRNPSRAACQMLCRWLDACLAGSRSSHPCGIMQTPCACLGGDTSDSASGSLAPRGDVCLRDGVAGGPENCLTSLTQDRGGDGTPGGWKLWSLWGECTRDCGGGLQTRTRTCLPAPGIQGGGCEGVLEEGRLCNRKACGPAGRTNSRSQSLRSTDARRREELGDDLQQFGFPAPQTGDPAAEEWSPWSVCSSTCGEGWQTRTRFCVSTSYSTQCSGPLREQRLCNNSAVCPVHGAWDEWSPWSLCSSTCGRGFRDRTRTCRPPQFGGNPCEGPEKQTKFCNIALCPVDGNWNEWSSWSTCSASCSQGRQQRTRECNGPSYGGAECQGHWVETRDCFLQQCPVDGKWQAWASWGSCSVTCGGGTQRRERACLGPFFGGVACQGPQDEYRQCGAQRCPEPHEICDEDNFGAVVWKETPAGEVAAVRCPRNATGLILRRCELDEEGIAYWEPPTYIRCVSIDYRNIQMMTREHLAKAQRGLPGEGVSEVIQTLVEISQDGTSYSGDLLSTIDVLRNMTEIFRRAYYSPTPGDVQNFVQIISNLLAEENRDKWEEAQLMGPNAKELFRLVEDFVDVIGFRMKDLRDAYQVTDNLVLSIHKLPASGATDISFPMKGWRATGDWAKVPEDRVTVSKSVFSTGLAEADDSSVFVVGTVLYRNLGSFLALQRNTTVLNSKVISVTVKPPPRSLLTPLEIEFAHMYNGTTNQTCILWDETDPPSSPAPPQLGPWSWRGCRTVPLDALRTRCLCDRLSTFAILAQLSADASMEKVATPSVTLIVGCGVSSLTLLMLIIIYVSVWRYIRSERSVILINFCLSIISSNALILIGQTQTRNKAVCTLVAAFLHFFFLSSFCWVLTEAWQSYMAVTGRLRNRLVRKRFLCLGWGLPALVVAISVGFTKARGYSTMNYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSKDGITDKKLKERAGASLWSSCVVLPLLALTWMSAVLAVTDRRSALFQILFAVFDSLEGFVIVMVHCILRREVQDAVKCRVVDRQEEGNGDSGGSFQNGHAQLMTDFEKDVDLACRSVLNKDIAACRTATITGTLKRPSQPEEEKLKLAHAKAPSNFNSLPANVSKLHLHGSPRCPGGPLPDFPNHSLTLKKDRAPKSSFVGDGDIFKKLDSELSRAQEKALDTSYVILPTATATLRPKPPEEPKYSIHIDQMPQTRLIHLSMAPDAGLPARSPPSRQPPGSGPPEPPPTQPPPPPPPPPPPPQQPLPPPPPMEPAPPSLGEPGEPAAHQGPSTGPGTKTENVSTLSVSSLERRKSRYAELDFEKIMHTRKRHQDMFQDLNRKLQHAEKDKDVLGPDSKPEKQQTPNKRPWESLRKAHGPPAWVKKELEPLPPSPLELRSVEWEKSGATIPLVGQDIIDLQTEV

Target ORF information:

RefSeq Version	XM_010811854.3
Organism	Bos taurus(cattle)
Definition	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811854.3

ORF Insert Sequence:

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCCGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGGTACAGC ACCATGAACT ACTGCTGGCT CTCCCTGGAG 
GGGGGACTGC TGTACGCCTT CGTGGGACCG GCCGCGGCCG TCGTGCTGGT GAACATGGTC 
ATCGGGATCC TGGTGTTCAA CAAGCTCGTG TCCAAGGACG GCATCACGGA CAAGAAGCTG 
AAGGAGCGGG CAGGGGCCTC GCTGTGGAGC TCCTGCGTGG TGCTGCCGCT GCTGGCGCTG 
ACCTGGATGT CTGCTGTGCT CGCCGTCACC GACCGCAGGT CCGCCCTCTT CCAGATCCTC 
TTTGCTGTCT TCGACTCGCT GGAGGGCTTC GTCATCGTGA TGGTGCACTG CATCCTACGG 
AGAGAGGTCC AGGACGCTGT GAAGTGCCGT GTGGTGGACC GCCAGGAGGA GGGCAACGGG 
GACTCAGGGG GCTCCTTCCA GAACGGCCAC GCCCAGCTCA TGACCGACTT TGAGAAGGAC 
GTGGATCTGG CCTGTAGATC AGTGCTGAAC AAGGACATTG CAGCTTGCCG CACGGCCACC 
ATCACAGGCA CGCTCAAACG GCCATCGCAG CCGGAGGAGG AGAAGCTGAA GCTGGCCCAT 
GCCAAGGCGC CCTCCAACTT CAACAGCCTG CCGGCCAATG TATCCAAGCT GCACCTGCAC 
GGCTCGCCCC GCTGCCCCGG CGGGCCCCTG CCCGACTTCC CCAACCACTC CCTGACCCTC 
AAGAAAGACA GGGCGCCCAA GTCGTCGTTC GTTGGCGACG GGGACATCTT CAAGAAGCTG 
GACTCGGAGC TGAGCCGGGC CCAGGAGAAA GCCCTGGACA CAAGCTACGT TATCCTGCCC 
ACGGCCACGG CCACGCTGCG GCCCAAGCCC CCGGAGGAGC CCAAGTACAG CATCCACATT 
GACCAGATGC CCCAGACCCG CCTCATCCAC CTCAGCATGG CGCCTGACGC GGGGCTCCCG 
GCCCGCAGCC CGCCCTCCCG CCAGCCCCCC GGCAGCGGGC CCCCCGAGCC GCCCCCCACC 
CAGCCCCCGC CGCCTCCGCC CCCGCCACCC CCACCGCCCC AGCAGCCCCT GCCCCCACCG 
CCCCCCATGG AGCCAGCACC CCCCAGCCTG GGGGAGCCCG GGGAGCCTGC TGCCCACCAG 
GGGCCCAGTA CGGGGCCCGG GACAAAGACA GAGAATGTCT CCACGCTGTC CGTGAGCTCC 
CTGGAGCGGC GGAAATCACG GTATGCGGAA CTGGACTTTG AGAAAATCAT GCACACACGG 
AAACGGCACC AGGACATGTT CCAGGACCTG AACCGGAAGT TACAGCACGC AGAGAAGGAC 
AAGGATGTGC TGGGCCCAGA CAGCAAGCCA GAAAAGCAGC AGACGCCCAA CAAGCGGCCC 
TGGGAGAGCC TCCGGAAAGC GCACGGGCCG CCCGCCTGGG TGAAGAAGGA GCTGGAGCCG 
CTCCCACCGT CCCCACTGGA GCTGCGGAGT GTGGAGTGGG AGAAGTCGGG CGCCACCATC 
CCGCTGGTGG GCCAGGACAT CATCGACCTC CAGACCGAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB1 ( XM_025001588.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857356.1 Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X4, mRNA.

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CloneID	OBa222312
Clone ID Related Accession (Same CDS sequence)	XM_025001588.1
Accession Version	XM_025001588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adhesion G protein-coupled receptor B1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCCGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGCTGCTGG CTCTCCCTGG AGGGGGGACT GCTGTACGCC 
TTCGTGGGAC CGGCCGCGGC CGTCGTGCTG GTGAACATGG TCATCGGGAT CCTGGTGTTC 
AACAAGCTCG TGTCCAAGGA CGGCATCACG GACAAGAAGC TGAAGGAGCG GGCAGGGGCC 
TCGCTGTGGA GCTCCTGCGT GGTGCTGCCG CTGCTGGCGC TGACCTGGAT GTCTGCTGTG 
CTCGCCGTCA CCGACCGCAG GTCCGCCCTC TTCCAGATCC TCTTTGCTGT CTTCGACTCG 
CTGGAGGGCT TCGTCATCGT GATGGTGCAC TGCATCCTAC GGAGAGAGGT CCAGGACGCT 
GTGAAGTGCC GTGTGGTGGA CCGCCAGGAG GAGGGCAACG GGGACTCAGG GGGCTCCTTC 
CAGAACGGCC ACGCCCAGCT CATGACCGAC TTTGAGAAGG ACGTGGATCT GGCCTGTAGA 
TCAGGTGAGC GCCTGACAGT GCTGAACAAG GACATTGCAG CTTGCCGCAC GGCCACCATC 
ACAGGCACGC TCAAACGGCC ATCGCAGCCG GAGGAGGAGA AGCTGAAGCT GGCCCATGCC 
AAGGCGCCCT CCAACTTCAA CAGCCTGCCG GCCAATGTAT CCAAGCTGCA CCTGCACGGC 
TCGCCCCGCT GCCCCGGCGG GCCCCTGCCC GACTTCCCCA ACCACTCCCT GACCCTCAAG 
AAAGACAGGG CGCCCAAGTC GTCGTTCGTT GGCGACGGGG ACATCTTCAA GAAGCTGGAC 
TCGGAGCTGA GCCGGGCCCA GGAGAAAGCC CTGGACACAA GCTACGTTAT CCTGCCCACG 
GCCACGGCCA CGCTGCGGCC CAAGCCCCCG GAGGAGCCCA AGTACAGCAT CCACATTGAC 
CAGATGCCCC AGACCCGCCT CATCCACCTC AGCATGGCGC CTGACGCGGG GCTCCCGGCC 
CGCAGCCCGC CCTCCCGCCA GCCCCCCGGC AGCGGGCCCC CCGAGCCGCC CCCCACCCAG 
CCCCCGCCGC CTCCGCCCCC GCCACCCCCA CCGCCCCAGC AGCCCCTGCC CCCACCGCCC 
CCCATGGAGC CAGCACCCCC CAGCCTGGGG GAGCCCGGGG AGCCTGCTGC CCACCAGGGG 
CCCAGTACGG GGCCCGGGAC AAAGACAGAG AATGTCTCCA CGCTGTCCGT GAGCTCCCTG 
GAGCGGCGGA AATCACGGTA TGCGGAACTG GACTTTGAGA AAATCATGCA CACACGGAAA 
CGGCACCAGG ACATGTTCCA GGACCTGAAC CGGAAGTTAC AGCACGCAGA GAAGGACAAG 
GATGTGCTGG GCCCAGACAG CAAGCCAGAA AAGCAGCAGA CGCCCAACAA GCGGCCCTGG 
GAGAGCCTCC GGAAAGCGCA CGGGCCGCCC GCCTGGGTGA AGAAGGAGCT GGAGCCGCTC 
CCACCGTCCC CACTGGAGCT GCGGAGTGTG GAGTGGGAGA AGTCGGGCGC CACCATCCCG 
CTGGTGGGCC AGGACATCAT CGACCTCCAG ACCGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857356.1
CDS	343..5064
Translation	MRGQAAGPGALWILGLLLLLGRQVGTAAGTAAGPGAEPCATLVQGKFFGYFSAAAVFPANASRCSWTLRNPDPRRYTLYMKVAKAPACSGPARVRTYQFDSFLESTRTYLGVESFDEVLRLCDPSAPLAFLQASKQFLQMQRLRPPQDNTLRTRDGPPGPSDDFSVEYLVVGNRNPSRAACQMLCRWLDACLAGSRSSHPCGIMQTPCACLGGDTSDSASGSLAPRGDVCLRDGVAGGPENCLTSLTQDRGGDGTPGGWKLWSLWGECTRDCGGGLQTRTRTCLPAPGIQGGGCEGVLEEGRLCNRKACGPAGRTNSRSQSLRSTDARRREELGDDLQQFGFPAPQTGDPAAEEWSPWSVCSSTCGEGWQTRTRFCVSTSYSTQCSGPLREQRLCNNSAVCPVHGAWDEWSPWSLCSSTCGRGFRDRTRTCRPPQFGGNPCEGPEKQTKFCNIALCPVDGNWNEWSSWSTCSASCSQGRQQRTRECNGPSYGGAECQGHWVETRDCFLQQCPVDGKWQAWASWGSCSVTCGGGTQRRERACLGPFFGGVACQGPQDEYRQCGAQRCPEPHEICDEDNFGAVVWKETPAGEVAAVRCPRNATGLILRRCELDEEGIAYWEPPTYIRCVSIDYRNIQMMTREHLAKAQRGLPGEGVSEVIQTLVEISQDGTSYSGDLLSTIDVLRNMTEIFRRAYYSPTPGDVQNFVQIISNLLAEENRDKWEEAQLMGPNAKELFRLVEDFVDVIGFRMKDLRDAYQVTDNLVLSIHKLPASGATDISFPMKGWRATGDWAKVPEDRVTVSKSVFSTGLAEADDSSVFVVGTVLYRNLGSFLALQRNTTVLNSKVISVTVKPPPRSLLTPLEIEFAHMYNGTTNQTCILWDETDPPSSPAPPQLGPWSWRGCRTVPLDALRTRCLCDRLSTFAILAQLSADASMEKVATPSVTLIVGCGVSSLTLLMLIIIYVSVWRYIRSERSVILINFCLSIISSNALILIGQTQTRNKAVCTLVAAFLHFFFLSSFCWVLTEAWQSYMAVTGRLRNRLVRKRFLCLGWGLPALVVAISVGFTKARGCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSKDGITDKKLKERAGASLWSSCVVLPLLALTWMSAVLAVTDRRSALFQILFAVFDSLEGFVIVMVHCILRREVQDAVKCRVVDRQEEGNGDSGGSFQNGHAQLMTDFEKDVDLACRSGERLTVLNKDIAACRTATITGTLKRPSQPEEEKLKLAHAKAPSNFNSLPANVSKLHLHGSPRCPGGPLPDFPNHSLTLKKDRAPKSSFVGDGDIFKKLDSELSRAQEKALDTSYVILPTATATLRPKPPEEPKYSIHIDQMPQTRLIHLSMAPDAGLPARSPPSRQPPGSGPPEPPPTQPPPPPPPPPPPPQQPLPPPPPMEPAPPSLGEPGEPAAHQGPSTGPGTKTENVSTLSVSSLERRKSRYAELDFEKIMHTRKRHQDMFQDLNRKLQHAEKDKDVLGPDSKPEKQQTPNKRPWESLRKAHGPPAWVKKELEPLPPSPLELRSVEWEKSGATIPLVGQDIIDLQTEV

Target ORF information:

RefSeq Version	XM_025001588.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001588.1

ORF Insert Sequence:

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCCGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGCTGCTGG CTCTCCCTGG AGGGGGGACT GCTGTACGCC 
TTCGTGGGAC CGGCCGCGGC CGTCGTGCTG GTGAACATGG TCATCGGGAT CCTGGTGTTC 
AACAAGCTCG TGTCCAAGGA CGGCATCACG GACAAGAAGC TGAAGGAGCG GGCAGGGGCC 
TCGCTGTGGA GCTCCTGCGT GGTGCTGCCG CTGCTGGCGC TGACCTGGAT GTCTGCTGTG 
CTCGCCGTCA CCGACCGCAG GTCCGCCCTC TTCCAGATCC TCTTTGCTGT CTTCGACTCG 
CTGGAGGGCT TCGTCATCGT GATGGTGCAC TGCATCCTAC GGAGAGAGGT CCAGGACGCT 
GTGAAGTGCC GTGTGGTGGA CCGCCAGGAG GAGGGCAACG GGGACTCAGG GGGCTCCTTC 
CAGAACGGCC ACGCCCAGCT CATGACCGAC TTTGAGAAGG ACGTGGATCT GGCCTGTAGA 
TCAGGTGAGC GCCTGACAGT GCTGAACAAG GACATTGCAG CTTGCCGCAC GGCCACCATC 
ACAGGCACGC TCAAACGGCC ATCGCAGCCG GAGGAGGAGA AGCTGAAGCT GGCCCATGCC 
AAGGCGCCCT CCAACTTCAA CAGCCTGCCG GCCAATGTAT CCAAGCTGCA CCTGCACGGC 
TCGCCCCGCT GCCCCGGCGG GCCCCTGCCC GACTTCCCCA ACCACTCCCT GACCCTCAAG 
AAAGACAGGG CGCCCAAGTC GTCGTTCGTT GGCGACGGGG ACATCTTCAA GAAGCTGGAC 
TCGGAGCTGA GCCGGGCCCA GGAGAAAGCC CTGGACACAA GCTACGTTAT CCTGCCCACG 
GCCACGGCCA CGCTGCGGCC CAAGCCCCCG GAGGAGCCCA AGTACAGCAT CCACATTGAC 
CAGATGCCCC AGACCCGCCT CATCCACCTC AGCATGGCGC CTGACGCGGG GCTCCCGGCC 
CGCAGCCCGC CCTCCCGCCA GCCCCCCGGC AGCGGGCCCC CCGAGCCGCC CCCCACCCAG 
CCCCCGCCGC CTCCGCCCCC GCCACCCCCA CCGCCCCAGC AGCCCCTGCC CCCACCGCCC 
CCCATGGAGC CAGCACCCCC CAGCCTGGGG GAGCCCGGGG AGCCTGCTGC CCACCAGGGG 
CCCAGTACGG GGCCCGGGAC AAAGACAGAG AATGTCTCCA CGCTGTCCGT GAGCTCCCTG 
GAGCGGCGGA AATCACGGTA TGCGGAACTG GACTTTGAGA AAATCATGCA CACACGGAAA 
CGGCACCAGG ACATGTTCCA GGACCTGAAC CGGAAGTTAC AGCACGCAGA GAAGGACAAG 
GATGTGCTGG GCCCAGACAG CAAGCCAGAA AAGCAGCAGA CGCCCAACAA GCGGCCCTGG 
GAGAGCCTCC GGAAAGCGCA CGGGCCGCCC GCCTGGGTGA AGAAGGAGCT GGAGCCGCTC 
CCACCGTCCC CACTGGAGCT GCGGAGTGTG GAGTGGGAGA AGTCGGGCGC CACCATCCCG 
CTGGTGGGCC AGGACATCAT CGACCTCCAG ACCGAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB1 ( NM_001191541.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178470.1 Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), mRNA.

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CloneID	OBa41030
Clone ID Related Accession (Same CDS sequence)	NM_001191541.1
Accession Version	NM_001191541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	brain-specific angiogenesis inhibitor 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02037483.1 and DAAA02037482.1. On Apr 20, 2011 this sequence version replaced XM_002705893.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02403639, SAMN02403640 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCGGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGGCCGGGCA 
GTGGATGGAA ACTGGAACGA GTGGTCCAGC TGGAGCACGT GCTCCGCCAG CTGCTCCCAG 
GGCCGGCAGC AGCGCACACG CGAGTGCAAC GGGCCTTCGT ATGGGGGTGC TGAGTGCCAG 
GGCCACTGGG TAGAGACGCG AGACTGCTTC CTGCAGCAGT GTCCAGTGGA TGGGAAGTGG 
CAGGCCTGGG CGTCCTGGGG CAGCTGCAGC GTCACATGCG GGGGTGGCAC GCAGCGGCGG 
GAGCGTGCCT GCTTGGGGCC CTTCTTTGGG GGAGTGGCCT GCCAGGGCCC CCAGGATGAA 
TACCGGCAGT GTGGCGCCCA GCGGTGTCCC GAGCCCCACG AGATCTGTGA TGAGGACAAC 
TTCGGAGCTG TGGTCTGGAA GGAGACCCCG GCCGGAGAGG TGGCTGCAGT CCGGTGTCCC 
CGCAACGCCA CAGGCCTCAT CCTGCGACGC TGTGAGCTGG ATGAAGAAGG CATTGCCTAC 
TGGGAGCCCC CCACCTATAT CCGCTGTGTG TCGATTGACT ACCGGAACAT CCAAATGATG 
ACCCGGGAGC ACCTAGCGAA GGCGCAGCGT GGGCTGCCCG GGGAGGGCGT CTCCGAGGTC 
ATCCAGACCC TGGTGGAGAT CTCCCAGGAC GGGACCAGCT ACAGCGGGGA CCTGCTGTCC 
ACCATCGACG TCCTAAGGAA CATGACAGAG ATCTTCCGCC GGGCGTACTA CAGCCCGACC 
CCTGGGGACG TGCAGAACTT TGTGCAGATC ATCAGCAACT TGCTGGCTGA GGAGAATCGG 
GACAAGTGGG AGGAGGCGCA GCTGATGGGC CCCAATGCCA AGGAGCTCTT CCGGCTAGTG 
GAGGACTTCG TGGACGTCAT CGGCTTCCGC ATGAAAGACT TGCGGGACGC GTACCAGGTG 
ACAGACAACC TGGTCCTCAG CATCCACAAG CTCCCAGCCA GCGGAGCCAC CGACATCAGC 
TTCCCCATGA AGGGCTGGCG GGCCACAGGC GACTGGGCCA AGGTGCCGGA GGACAGGGTC 
ACTGTGTCCA AGAGTGTCTT CTCCACGGGG CTGGCAGAGG CTGATGACTC GTCTGTGTTC 
GTGGTGGGCA CTGTGCTTTA CCGAAACCTG GGCAGCTTCC TGGCCCTGCA GAGGAACACG 
ACTGTCCTGA ACTCCAAGGT CATCTCGGTG ACGGTGAAGC CCCCGCCTCG CTCCCTGCTC 
ACACCCTTGG AGATCGAGTT CGCCCACATG TACAACGGCA CCACCAACCA GACCTGCATC 
CTGTGGGACG AGACAGACCC ACCCTCCTCC CCCGCCCCCC CGCAGCTCGG GCCCTGGTCG 
TGGCGCGGCT GCCGCACGGT GCCCCTCGAC GCCCTCCGGA CGCGCTGCCT CTGTGACCGG 
CTCTCCACCT TCGCCATCTT AGCCCAGCTC AGCGCCGACG CGAGCATGGA GAAGGTGGCC 
ACGCCGTCGG TGACGCTCAT CGTGGGCTGC GGCGTGTCGT CCCTGACCCT GCTCATGCTG 
ATCATCATCT ACGTCTCCGT GTGGAGGTAC ATCCGCTCGG AGCGCTCCGT CATCCTCATC 
AACTTCTGCC TGTCCATCAT CTCCTCCAAC GCGCTCATCC TCATCGGGCA GACCCAGACC 
CGCAACAAGG CCGTGTGCAC GCTGGTGGCC GCCTTCCTGC ACTTCTTCTT CCTGTCCTCC 
TTCTGCTGGG TGTTGACCGA GGCCTGGCAG TCCTACATGG CCGTGACTGG CCGCCTGCGG 
AACCGCCTCG TCCGCAAGCG CTTCCTGTGC CTGGGCTGGG GGCTCCCTGC GCTGGTCGTG 
GCTATTTCCG TGGGATTTAC CAAGGCCAGA GGGTACAGCA CCATGAACTA CTGCTGGCTC 
TCCCTGGAGG GGGGACTGCT GTACGCCTTC GTGGGACCGG CCGCGGCCGT CGTGCTGGTG 
AACATGGTCA TCGGGATCCT GGTGTTCAAC AAGCTCGTGT CCAAGGACGG CATCACGGAC 
AAGAAGCTGA AGGAGCGGGC AGGGGCCTCG CTGTGGAGCT CCTGCGTGGT GCTGCCGCTG 
CTGGCGCTGA CCTGGATGTC TGCTGTGCTC GCCGTCACCG ACCGCAGGTC CGCCCTCTTC 
CAGATCCTCT TTGCTGTCTT CGACTCGCTG GAGGGCTTCG TCATCGTGAT GGTGCACTGC 
ATCCTACGGA GAGAGGTCCA GGACGCTGTG AAGTGCCGTG TGGTGGACCG CCAGGAGGAG 
GGCAACGGGG ACTCAGGGGG CTCCTTCCAG AACGGCCACG CCCAGCTCAT GACCGACTTT 
GAGAAGGACG TGGATCTGGC CTGTAGATCA GTGCTGAACA AGGACATTGC AGCTTGCCGC 
ACGGCCACCA TCACAGGCAC GCTCAAACGG CCATCGCAGC CGGAGGAGGA GAAGCTGAAG 
CTGGCCCATG CCAAGGCGCC CTCCAACTTC AACAGCCTGC CGGCCAATGT ATCCAAGCTG 
CACCTGCACG GCTCGCCCCG CTGCCCCGGC GGGCCCCTGC CCGACTTCCC CAACCACTCC 
CTGACCCTCA AGAAAGACAG GGCGCCCAAG TCGTCGTTCG TTGGCGACGG GGACATCTTC 
AAGAAGCTGG ACTCGGAGCT GAGCCGGGCC CAGGAGAAAG CCCTGGACAC AAGCTACGTT 
ATCCTGCCCA CGGCCACGGC CACGCTGCGG CCCAAGCCCC CGGAGGAGCC CAAGTACAGC 
ATCCACATTG ACCAGATGCC CCAGACCCGC CTCATCCACC TCAGCATGGC GCCTGACGCG 
GGGCTCCCGG CCCGCAGCCC GCCCTCCCGC CAGCCCCCCG GCAGCGGGCC CCCCGAGCCG 
CCCCCCACCC AGCCCCCGCC GCCTCCGCCC CCGCCACCCC CACCGCCCCA GCAGCCCCTG 
CCCCCACCGC CCCCCATGGA GCCAGCACCC CCCAGCCTGG GGGAGCCCGG GGAGCCTGCT 
GCCCACCAGG GGCCCAGTAC GGGGCCCGGG ACAAAGACAG AGAATGTCTC CACGCTGTCC 
GTGAGCTCCC TGGAGCGGCG GAAATCACGG TATGCGGAAC TGGACTTTGA GAAAATCATG 
CACACACGGA AACGGCACCA GGACATGTTC CAGGACCTGA ACCGGAAGTT ACAGCACGCA 
GAGAAGGACA AGGATGTGCT GGGCCCAGAC AGCAAGCCAG AAAAGCAGCA GACGCCCAAC 
AAGCGGCCCT GGGAGAGCCT CCGGAAAGCG CACGGGCCGC CCGCCTGGGT GAAGAAGGAG 
CTGGAGCCGC TCCCACCGTC CCCACTGGAG CTGCGGAGTG TGGAGTGGGA GAAGTCGGGC 
GCCACCATCC CGCTGGTGGG CCAGGACATC ATCGACCTCC AGACCGAGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178470.1
CDS	1..4734
Translation	MRGQAAGPGALWILGLLLLLGRQVGTAAGTAAGPGAEPCATLVQGKFFGYFSAAAVFPANASRCSWTLRNPDPRRYTLYMKVAKAPACSGPARVRTYQFDSFLESTRTYLGVESFDEVLRLCDPSAPLAFLQASKQFLQMQRLRPPQDNTLRTRDGPPGPSDDFSVEYLVVGNRNPSRAACQMLCRWLDACLAGSRSSHPCGIMQTPCACLGGDTSDSASGSLAPRGDVCLRDGVAGGPENCLTSLTQDRGGDGTPGGWKLWSLWGECTRDCGGGLQTRTRTCLPAPGIQGGGCEGVLEEGRLCNRKACGPAGRTNSRSQSLRSTDARRREELGDDLQQFGFPAPQTGDPAAEEWSPWSVCSSTCGEGWQTRTRFCVSTSYSTQCSGPLREQRLCNNSAVCPVHGAWDEWSPWSLCSSTCGRGFRDRTRTCRPPQFGGNPCEGPEKQTKFCNIALCPGRAVDGNWNEWSSWSTCSASCSQGRQQRTRECNGPSYGGAECQGHWVETRDCFLQQCPVDGKWQAWASWGSCSVTCGGGTQRRERACLGPFFGGVACQGPQDEYRQCGAQRCPEPHEICDEDNFGAVVWKETPAGEVAAVRCPRNATGLILRRCELDEEGIAYWEPPTYIRCVSIDYRNIQMMTREHLAKAQRGLPGEGVSEVIQTLVEISQDGTSYSGDLLSTIDVLRNMTEIFRRAYYSPTPGDVQNFVQIISNLLAEENRDKWEEAQLMGPNAKELFRLVEDFVDVIGFRMKDLRDAYQVTDNLVLSIHKLPASGATDISFPMKGWRATGDWAKVPEDRVTVSKSVFSTGLAEADDSSVFVVGTVLYRNLGSFLALQRNTTVLNSKVISVTVKPPPRSLLTPLEIEFAHMYNGTTNQTCILWDETDPPSSPAPPQLGPWSWRGCRTVPLDALRTRCLCDRLSTFAILAQLSADASMEKVATPSVTLIVGCGVSSLTLLMLIIIYVSVWRYIRSERSVILINFCLSIISSNALILIGQTQTRNKAVCTLVAAFLHFFFLSSFCWVLTEAWQSYMAVTGRLRNRLVRKRFLCLGWGLPALVVAISVGFTKARGYSTMNYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSKDGITDKKLKERAGASLWSSCVVLPLLALTWMSAVLAVTDRRSALFQILFAVFDSLEGFVIVMVHCILRREVQDAVKCRVVDRQEEGNGDSGGSFQNGHAQLMTDFEKDVDLACRSVLNKDIAACRTATITGTLKRPSQPEEEKLKLAHAKAPSNFNSLPANVSKLHLHGSPRCPGGPLPDFPNHSLTLKKDRAPKSSFVGDGDIFKKLDSELSRAQEKALDTSYVILPTATATLRPKPPEEPKYSIHIDQMPQTRLIHLSMAPDAGLPARSPPSRQPPGSGPPEPPPTQPPPPPPPPPPPPQQPLPPPPPMEPAPPSLGEPGEPAAHQGPSTGPGTKTENVSTLSVSSLERRKSRYAELDFEKIMHTRKRHQDMFQDLNRKLQHAEKDKDVLGPDSKPEKQQTPNKRPWESLRKAHGPPAWVKKELEPLPPSPLELRSVEWEKSGATIPLVGQDIIDLQTEV

Target ORF information:

RefSeq Version	NM_001191541.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191541.1

ORF Insert Sequence:

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCGGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGGCCGGGCA 
GTGGATGGAA ACTGGAACGA GTGGTCCAGC TGGAGCACGT GCTCCGCCAG CTGCTCCCAG 
GGCCGGCAGC AGCGCACACG CGAGTGCAAC GGGCCTTCGT ATGGGGGTGC TGAGTGCCAG 
GGCCACTGGG TAGAGACGCG AGACTGCTTC CTGCAGCAGT GTCCAGTGGA TGGGAAGTGG 
CAGGCCTGGG CGTCCTGGGG CAGCTGCAGC GTCACATGCG GGGGTGGCAC GCAGCGGCGG 
GAGCGTGCCT GCTTGGGGCC CTTCTTTGGG GGAGTGGCCT GCCAGGGCCC CCAGGATGAA 
TACCGGCAGT GTGGCGCCCA GCGGTGTCCC GAGCCCCACG AGATCTGTGA TGAGGACAAC 
TTCGGAGCTG TGGTCTGGAA GGAGACCCCG GCCGGAGAGG TGGCTGCAGT CCGGTGTCCC 
CGCAACGCCA CAGGCCTCAT CCTGCGACGC TGTGAGCTGG ATGAAGAAGG CATTGCCTAC 
TGGGAGCCCC CCACCTATAT CCGCTGTGTG TCGATTGACT ACCGGAACAT CCAAATGATG 
ACCCGGGAGC ACCTAGCGAA GGCGCAGCGT GGGCTGCCCG GGGAGGGCGT CTCCGAGGTC 
ATCCAGACCC TGGTGGAGAT CTCCCAGGAC GGGACCAGCT ACAGCGGGGA CCTGCTGTCC 
ACCATCGACG TCCTAAGGAA CATGACAGAG ATCTTCCGCC GGGCGTACTA CAGCCCGACC 
CCTGGGGACG TGCAGAACTT TGTGCAGATC ATCAGCAACT TGCTGGCTGA GGAGAATCGG 
GACAAGTGGG AGGAGGCGCA GCTGATGGGC CCCAATGCCA AGGAGCTCTT CCGGCTAGTG 
GAGGACTTCG TGGACGTCAT CGGCTTCCGC ATGAAAGACT TGCGGGACGC GTACCAGGTG 
ACAGACAACC TGGTCCTCAG CATCCACAAG CTCCCAGCCA GCGGAGCCAC CGACATCAGC 
TTCCCCATGA AGGGCTGGCG GGCCACAGGC GACTGGGCCA AGGTGCCGGA GGACAGGGTC 
ACTGTGTCCA AGAGTGTCTT CTCCACGGGG CTGGCAGAGG CTGATGACTC GTCTGTGTTC 
GTGGTGGGCA CTGTGCTTTA CCGAAACCTG GGCAGCTTCC TGGCCCTGCA GAGGAACACG 
ACTGTCCTGA ACTCCAAGGT CATCTCGGTG ACGGTGAAGC CCCCGCCTCG CTCCCTGCTC 
ACACCCTTGG AGATCGAGTT CGCCCACATG TACAACGGCA CCACCAACCA GACCTGCATC 
CTGTGGGACG AGACAGACCC ACCCTCCTCC CCCGCCCCCC CGCAGCTCGG GCCCTGGTCG 
TGGCGCGGCT GCCGCACGGT GCCCCTCGAC GCCCTCCGGA CGCGCTGCCT CTGTGACCGG 
CTCTCCACCT TCGCCATCTT AGCCCAGCTC AGCGCCGACG CGAGCATGGA GAAGGTGGCC 
ACGCCGTCGG TGACGCTCAT CGTGGGCTGC GGCGTGTCGT CCCTGACCCT GCTCATGCTG 
ATCATCATCT ACGTCTCCGT GTGGAGGTAC ATCCGCTCGG AGCGCTCCGT CATCCTCATC 
AACTTCTGCC TGTCCATCAT CTCCTCCAAC GCGCTCATCC TCATCGGGCA GACCCAGACC 
CGCAACAAGG CCGTGTGCAC GCTGGTGGCC GCCTTCCTGC ACTTCTTCTT CCTGTCCTCC 
TTCTGCTGGG TGTTGACCGA GGCCTGGCAG TCCTACATGG CCGTGACTGG CCGCCTGCGG 
AACCGCCTCG TCCGCAAGCG CTTCCTGTGC CTGGGCTGGG GGCTCCCTGC GCTGGTCGTG 
GCTATTTCCG TGGGATTTAC CAAGGCCAGA GGGTACAGCA CCATGAACTA CTGCTGGCTC 
TCCCTGGAGG GGGGACTGCT GTACGCCTTC GTGGGACCGG CCGCGGCCGT CGTGCTGGTG 
AACATGGTCA TCGGGATCCT GGTGTTCAAC AAGCTCGTGT CCAAGGACGG CATCACGGAC 
AAGAAGCTGA AGGAGCGGGC AGGGGCCTCG CTGTGGAGCT CCTGCGTGGT GCTGCCGCTG 
CTGGCGCTGA CCTGGATGTC TGCTGTGCTC GCCGTCACCG ACCGCAGGTC CGCCCTCTTC 
CAGATCCTCT TTGCTGTCTT CGACTCGCTG GAGGGCTTCG TCATCGTGAT GGTGCACTGC 
ATCCTACGGA GAGAGGTCCA GGACGCTGTG AAGTGCCGTG TGGTGGACCG CCAGGAGGAG 
GGCAACGGGG ACTCAGGGGG CTCCTTCCAG AACGGCCACG CCCAGCTCAT GACCGACTTT 
GAGAAGGACG TGGATCTGGC CTGTAGATCA GTGCTGAACA AGGACATTGC AGCTTGCCGC 
ACGGCCACCA TCACAGGCAC GCTCAAACGG CCATCGCAGC CGGAGGAGGA GAAGCTGAAG 
CTGGCCCATG CCAAGGCGCC CTCCAACTTC AACAGCCTGC CGGCCAATGT ATCCAAGCTG 
CACCTGCACG GCTCGCCCCG CTGCCCCGGC GGGCCCCTGC CCGACTTCCC CAACCACTCC 
CTGACCCTCA AGAAAGACAG GGCGCCCAAG TCGTCGTTCG TTGGCGACGG GGACATCTTC 
AAGAAGCTGG ACTCGGAGCT GAGCCGGGCC CAGGAGAAAG CCCTGGACAC AAGCTACGTT 
ATCCTGCCCA CGGCCACGGC CACGCTGCGG CCCAAGCCCC CGGAGGAGCC CAAGTACAGC 
ATCCACATTG ACCAGATGCC CCAGACCCGC CTCATCCACC TCAGCATGGC GCCTGACGCG 
GGGCTCCCGG CCCGCAGCCC GCCCTCCCGC CAGCCCCCCG GCAGCGGGCC CCCCGAGCCG 
CCCCCCACCC AGCCCCCGCC GCCTCCGCCC CCGCCACCCC CACCGCCCCA GCAGCCCCTG 
CCCCCACCGC CCCCCATGGA GCCAGCACCC CCCAGCCTGG GGGAGCCCGG GGAGCCTGCT 
GCCCACCAGG GGCCCAGTAC GGGGCCCGGG ACAAAGACAG AGAATGTCTC CACGCTGTCC 
GTGAGCTCCC TGGAGCGGCG GAAATCACGG TATGCGGAAC TGGACTTTGA GAAAATCATG 
CACACACGGA AACGGCACCA GGACATGTTC CAGGACCTGA ACCGGAAGTT ACAGCACGCA 
GAGAAGGACA AGGATGTGCT GGGCCCAGAC AGCAAGCCAG AAAAGCAGCA GACGCCCAAC 
AAGCGGCCCT GGGAGAGCCT CCGGAAAGCG CACGGGCCGC CCGCCTGGGT GAAGAAGGAG 
CTGGAGCCGC TCCCACCGTC CCCACTGGAG CTGCGGAGTG TGGAGTGGGA GAAGTCGGGC 
GCCACCATCC CGCTGGTGGG CCAGGACATC ATCGACCTCC AGACCGAGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB1 ( XM_015474338.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329824.1 Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X2, mRNA.

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CloneID	OBa50811
Clone ID Related Accession (Same CDS sequence)	XM_015474338.1 , XM_010811854.2
Accession Version	XM_015474338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	brain-specific angiogenesis inhibitor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCGGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGGTACAGC ACCATGAACT ACTGCTGGCT CTCCCTGGAG 
GGGGGACTGC TGTACGCCTT CGTGGGACCG GCCGCGGCCG TCGTGCTGGT GAACATGGTC 
ATCGGGATCC TGGTGTTCAA CAAGCTCGTG TCCAAGGACG GCATCACGGA CAAGAAGCTG 
AAGGAGCGGG CAGGGGCCTC GCTGTGGAGC TCCTGCGTGG TGCTGCCGCT GCTGGCGCTG 
ACCTGGATGT CTGCTGTGCT CGCCGTCACC GACCGCAGGT CCGCCCTCTT CCAGATCCTC 
TTTGCTGTCT TCGACTCGCT GGAGGGCTTC GTCATCGTGA TGGTGCACTG CATCCTACGG 
AGAGAGGTCC AGGACGCTGT GAAGTGCCGT GTGGTGGACC GCCAGGAGGA GGGCAACGGG 
GACTCAGGGG GCTCCTTCCA GAACGGCCAC GCCCAGCTCA TGACCGACTT TGAGAAGGAC 
GTGGATCTGG CCTGTAGATC AGTGCTGAAC AAGGACATTG CAGCTTGCCG CACGGCCACC 
ATCACAGGCA CGCTCAAACG GCCATCGCAG CCGGAGGAGG AGAAGCTGAA GCTGGCCCAT 
GCCAAGGCGC CCTCCAACTT CAACAGCCTG CCGGCCAATG TATCCAAGCT GCACCTGCAC 
GGCTCGCCCC GCTGCCCCGG CGGGCCCCTG CCCGACTTCC CCAACCACTC CCTGACCCTC 
AAGAAAGACA GGGCGCCCAA GTCGTCGTTC GTTGGCGACG GGGACATCTT CAAGAAGCTG 
GACTCGGAGC TGAGCCGGGC CCAGGAGAAA GCCCTGGACA CAAGCTACGT TATCCTGCCC 
ACGGCCACGG CCACGCTGCG GCCCAAGCCC CCGGAGGAGC CCAAGTACAG CATCCACATT 
GACCAGATGC CCCAGACCCG CCTCATCCAC CTCAGCATGG CGCCTGACGC GGGGCTCCCG 
GCCCGCAGCC CGCCCTCCCG CCAGCCCCCC GGCAGCGGGC CCCCCGAGCC GCCCCCCACC 
CAGCCCCCGC CGCCTCCGCC CCCGCCACCC CCACCGCCCC AGCAGCCCCT GCCCCCACCG 
CCCCCCATGG AGCCAGCACC CCCCAGCCTG GGGGAGCCCG GGGAGCCTGC TGCCCACCAG 
GGGCCCAGTA CGGGGCCCGG GACAAAGACA GAGAATGTCT CCACGCTGTC CGTGAGCTCC 
CTGGAGCGGC GGAAATCACG GTATGCGGAA CTGGACTTTG AGAAAATCAT GCACACACGG 
AAACGGCACC AGGACATGTT CCAGGACCTG AACCGGAAGT TACAGCACGC AGAGAAGGAC 
AAGGATGTGC TGGGCCCAGA CAGCAAGCCA GAAAAGCAGC AGACGCCCAA CAAGCGGCCC 
TGGGAGAGCC TCCGGAAAGC GCACGGGCCG CCCGCCTGGG TGAAGAAGGA GCTGGAGCCG 
CTCCCACCGT CCCCACTGGA GCTGCGGAGT GTGGAGTGGG AGAAGTCGGG CGCCACCATC 
CCGCTGGTGG GCCAGGACAT CATCGACCTC CAGACCGAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329824.1
CDS	262..4986
Translation	MRGQAAGPGALWILGLLLLLGRQVGTAAGTAAGPGAEPCATLVQGKFFGYFSAAAVFPANASRCSWTLRNPDPRRYTLYMKVAKAPACSGPARVRTYQFDSFLESTRTYLGVESFDEVLRLCDPSAPLAFLQASKQFLQMQRLRPPQDNTLRTRDGPPGPSDDFSVEYLVVGNRNPSRAACQMLCRWLDACLAGSRSSHPCGIMQTPCACLGGDTSDSASGSLAPRGDVCLRDGVAGGPENCLTSLTQDRGGDGTPGGWKLWSLWGECTRDCGGGLQTRTRTCLPAPGIQGGGCEGVLEEGRLCNRKACGPAGRTNSRSQSLRSTDARRREELGDDLQQFGFPAPQTGDPAAEEWSPWSVCSSTCGEGWQTRTRFCVSTSYSTQCSGPLREQRLCNNSAVCPVHGAWDEWSPWSLCSSTCGRGFRDRTRTCRPPQFGGNPCEGPEKQTKFCNIALCPVDGNWNEWSSWSTCSASCSQGRQQRTRECNGPSYGGAECQGHWVETRDCFLQQCPVDGKWQAWASWGSCSVTCGGGTQRRERACLGPFFGGVACQGPQDEYRQCGAQRCPEPHEICDEDNFGAVVWKETPAGEVAAVRCPRNATGLILRRCELDEEGIAYWEPPTYIRCVSIDYRNIQMMTREHLAKAQRGLPGEGVSEVIQTLVEISQDGTSYSGDLLSTIDVLRNMTEIFRRAYYSPTPGDVQNFVQIISNLLAEENRDKWEEAQLMGPNAKELFRLVEDFVDVIGFRMKDLRDAYQVTDNLVLSIHKLPASGATDISFPMKGWRATGDWAKVPEDRVTVSKSVFSTGLAEADDSSVFVVGTVLYRNLGSFLALQRNTTVLNSKVISVTVKPPPRSLLTPLEIEFAHMYNGTTNQTCILWDETDPPSSPAPPQLGPWSWRGCRTVPLDALRTRCLCDRLSTFAILAQLSADASMEKVATPSVTLIVGCGVSSLTLLMLIIIYVSVWRYIRSERSVILINFCLSIISSNALILIGQTQTRNKAVCTLVAAFLHFFFLSSFCWVLTEAWQSYMAVTGRLRNRLVRKRFLCLGWGLPALVVAISVGFTKARGYSTMNYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSKDGITDKKLKERAGASLWSSCVVLPLLALTWMSAVLAVTDRRSALFQILFAVFDSLEGFVIVMVHCILRREVQDAVKCRVVDRQEEGNGDSGGSFQNGHAQLMTDFEKDVDLACRSVLNKDIAACRTATITGTLKRPSQPEEEKLKLAHAKAPSNFNSLPANVSKLHLHGSPRCPGGPLPDFPNHSLTLKKDRAPKSSFVGDGDIFKKLDSELSRAQEKALDTSYVILPTATATLRPKPPEEPKYSIHIDQMPQTRLIHLSMAPDAGLPARSPPSRQPPGSGPPEPPPTQPPPPPPPPPPPPQQPLPPPPPMEPAPPSLGEPGEPAAHQGPSTGPGTKTENVSTLSVSSLERRKSRYAELDFEKIMHTRKRHQDMFQDLNRKLQHAEKDKDVLGPDSKPEKQQTPNKRPWESLRKAHGPPAWVKKELEPLPPSPLELRSVEWEKSGATIPLVGQDIIDLQTEV

Target ORF information:

RefSeq Version	XM_015474338.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474338.1

ORF Insert Sequence:

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCGGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGGTACAGC ACCATGAACT ACTGCTGGCT CTCCCTGGAG 
GGGGGACTGC TGTACGCCTT CGTGGGACCG GCCGCGGCCG TCGTGCTGGT GAACATGGTC 
ATCGGGATCC TGGTGTTCAA CAAGCTCGTG TCCAAGGACG GCATCACGGA CAAGAAGCTG 
AAGGAGCGGG CAGGGGCCTC GCTGTGGAGC TCCTGCGTGG TGCTGCCGCT GCTGGCGCTG 
ACCTGGATGT CTGCTGTGCT CGCCGTCACC GACCGCAGGT CCGCCCTCTT CCAGATCCTC 
TTTGCTGTCT TCGACTCGCT GGAGGGCTTC GTCATCGTGA TGGTGCACTG CATCCTACGG 
AGAGAGGTCC AGGACGCTGT GAAGTGCCGT GTGGTGGACC GCCAGGAGGA GGGCAACGGG 
GACTCAGGGG GCTCCTTCCA GAACGGCCAC GCCCAGCTCA TGACCGACTT TGAGAAGGAC 
GTGGATCTGG CCTGTAGATC AGTGCTGAAC AAGGACATTG CAGCTTGCCG CACGGCCACC 
ATCACAGGCA CGCTCAAACG GCCATCGCAG CCGGAGGAGG AGAAGCTGAA GCTGGCCCAT 
GCCAAGGCGC CCTCCAACTT CAACAGCCTG CCGGCCAATG TATCCAAGCT GCACCTGCAC 
GGCTCGCCCC GCTGCCCCGG CGGGCCCCTG CCCGACTTCC CCAACCACTC CCTGACCCTC 
AAGAAAGACA GGGCGCCCAA GTCGTCGTTC GTTGGCGACG GGGACATCTT CAAGAAGCTG 
GACTCGGAGC TGAGCCGGGC CCAGGAGAAA GCCCTGGACA CAAGCTACGT TATCCTGCCC 
ACGGCCACGG CCACGCTGCG GCCCAAGCCC CCGGAGGAGC CCAAGTACAG CATCCACATT 
GACCAGATGC CCCAGACCCG CCTCATCCAC CTCAGCATGG CGCCTGACGC GGGGCTCCCG 
GCCCGCAGCC CGCCCTCCCG CCAGCCCCCC GGCAGCGGGC CCCCCGAGCC GCCCCCCACC 
CAGCCCCCGC CGCCTCCGCC CCCGCCACCC CCACCGCCCC AGCAGCCCCT GCCCCCACCG 
CCCCCCATGG AGCCAGCACC CCCCAGCCTG GGGGAGCCCG GGGAGCCTGC TGCCCACCAG 
GGGCCCAGTA CGGGGCCCGG GACAAAGACA GAGAATGTCT CCACGCTGTC CGTGAGCTCC 
CTGGAGCGGC GGAAATCACG GTATGCGGAA CTGGACTTTG AGAAAATCAT GCACACACGG 
AAACGGCACC AGGACATGTT CCAGGACCTG AACCGGAAGT TACAGCACGC AGAGAAGGAC 
AAGGATGTGC TGGGCCCAGA CAGCAAGCCA GAAAAGCAGC AGACGCCCAA CAAGCGGCCC 
TGGGAGAGCC TCCGGAAAGC GCACGGGCCG CCCGCCTGGG TGAAGAAGGA GCTGGAGCCG 
CTCCCACCGT CCCCACTGGA GCTGCGGAGT GTGGAGTGGG AGAAGTCGGG CGCCACCATC 
CCGCTGGTGG GCCAGGACAT CATCGACCTC CAGACCGAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB1 ( XM_010811854.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810156.1 Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X1, mRNA.

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CloneID	OBa50811
Clone ID Related Accession (Same CDS sequence)	XM_015474338.1 , XM_010811854.2
Accession Version	XM_010811854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	brain-specific angiogenesis inhibitor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCGGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGGTACAGC ACCATGAACT ACTGCTGGCT CTCCCTGGAG 
GGGGGACTGC TGTACGCCTT CGTGGGACCG GCCGCGGCCG TCGTGCTGGT GAACATGGTC 
ATCGGGATCC TGGTGTTCAA CAAGCTCGTG TCCAAGGACG GCATCACGGA CAAGAAGCTG 
AAGGAGCGGG CAGGGGCCTC GCTGTGGAGC TCCTGCGTGG TGCTGCCGCT GCTGGCGCTG 
ACCTGGATGT CTGCTGTGCT CGCCGTCACC GACCGCAGGT CCGCCCTCTT CCAGATCCTC 
TTTGCTGTCT TCGACTCGCT GGAGGGCTTC GTCATCGTGA TGGTGCACTG CATCCTACGG 
AGAGAGGTCC AGGACGCTGT GAAGTGCCGT GTGGTGGACC GCCAGGAGGA GGGCAACGGG 
GACTCAGGGG GCTCCTTCCA GAACGGCCAC GCCCAGCTCA TGACCGACTT TGAGAAGGAC 
GTGGATCTGG CCTGTAGATC AGTGCTGAAC AAGGACATTG CAGCTTGCCG CACGGCCACC 
ATCACAGGCA CGCTCAAACG GCCATCGCAG CCGGAGGAGG AGAAGCTGAA GCTGGCCCAT 
GCCAAGGCGC CCTCCAACTT CAACAGCCTG CCGGCCAATG TATCCAAGCT GCACCTGCAC 
GGCTCGCCCC GCTGCCCCGG CGGGCCCCTG CCCGACTTCC CCAACCACTC CCTGACCCTC 
AAGAAAGACA GGGCGCCCAA GTCGTCGTTC GTTGGCGACG GGGACATCTT CAAGAAGCTG 
GACTCGGAGC TGAGCCGGGC CCAGGAGAAA GCCCTGGACA CAAGCTACGT TATCCTGCCC 
ACGGCCACGG CCACGCTGCG GCCCAAGCCC CCGGAGGAGC CCAAGTACAG CATCCACATT 
GACCAGATGC CCCAGACCCG CCTCATCCAC CTCAGCATGG CGCCTGACGC GGGGCTCCCG 
GCCCGCAGCC CGCCCTCCCG CCAGCCCCCC GGCAGCGGGC CCCCCGAGCC GCCCCCCACC 
CAGCCCCCGC CGCCTCCGCC CCCGCCACCC CCACCGCCCC AGCAGCCCCT GCCCCCACCG 
CCCCCCATGG AGCCAGCACC CCCCAGCCTG GGGGAGCCCG GGGAGCCTGC TGCCCACCAG 
GGGCCCAGTA CGGGGCCCGG GACAAAGACA GAGAATGTCT CCACGCTGTC CGTGAGCTCC 
CTGGAGCGGC GGAAATCACG GTATGCGGAA CTGGACTTTG AGAAAATCAT GCACACACGG 
AAACGGCACC AGGACATGTT CCAGGACCTG AACCGGAAGT TACAGCACGC AGAGAAGGAC 
AAGGATGTGC TGGGCCCAGA CAGCAAGCCA GAAAAGCAGC AGACGCCCAA CAAGCGGCCC 
TGGGAGAGCC TCCGGAAAGC GCACGGGCCG CCCGCCTGGG TGAAGAAGGA GCTGGAGCCG 
CTCCCACCGT CCCCACTGGA GCTGCGGAGT GTGGAGTGGG AGAAGTCGGG CGCCACCATC 
CCGCTGGTGG GCCAGGACAT CATCGACCTC CAGACCGAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810156.1
CDS	379..5103
Translation	MRGQAAGPGALWILGLLLLLGRQVGTAAGTAAGPGAEPCATLVQGKFFGYFSAAAVFPANASRCSWTLRNPDPRRYTLYMKVAKAPACSGPARVRTYQFDSFLESTRTYLGVESFDEVLRLCDPSAPLAFLQASKQFLQMQRLRPPQDNTLRTRDGPPGPSDDFSVEYLVVGNRNPSRAACQMLCRWLDACLAGSRSSHPCGIMQTPCACLGGDTSDSASGSLAPRGDVCLRDGVAGGPENCLTSLTQDRGGDGTPGGWKLWSLWGECTRDCGGGLQTRTRTCLPAPGIQGGGCEGVLEEGRLCNRKACGPAGRTNSRSQSLRSTDARRREELGDDLQQFGFPAPQTGDPAAEEWSPWSVCSSTCGEGWQTRTRFCVSTSYSTQCSGPLREQRLCNNSAVCPVHGAWDEWSPWSLCSSTCGRGFRDRTRTCRPPQFGGNPCEGPEKQTKFCNIALCPVDGNWNEWSSWSTCSASCSQGRQQRTRECNGPSYGGAECQGHWVETRDCFLQQCPVDGKWQAWASWGSCSVTCGGGTQRRERACLGPFFGGVACQGPQDEYRQCGAQRCPEPHEICDEDNFGAVVWKETPAGEVAAVRCPRNATGLILRRCELDEEGIAYWEPPTYIRCVSIDYRNIQMMTREHLAKAQRGLPGEGVSEVIQTLVEISQDGTSYSGDLLSTIDVLRNMTEIFRRAYYSPTPGDVQNFVQIISNLLAEENRDKWEEAQLMGPNAKELFRLVEDFVDVIGFRMKDLRDAYQVTDNLVLSIHKLPASGATDISFPMKGWRATGDWAKVPEDRVTVSKSVFSTGLAEADDSSVFVVGTVLYRNLGSFLALQRNTTVLNSKVISVTVKPPPRSLLTPLEIEFAHMYNGTTNQTCILWDETDPPSSPAPPQLGPWSWRGCRTVPLDALRTRCLCDRLSTFAILAQLSADASMEKVATPSVTLIVGCGVSSLTLLMLIIIYVSVWRYIRSERSVILINFCLSIISSNALILIGQTQTRNKAVCTLVAAFLHFFFLSSFCWVLTEAWQSYMAVTGRLRNRLVRKRFLCLGWGLPALVVAISVGFTKARGYSTMNYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSKDGITDKKLKERAGASLWSSCVVLPLLALTWMSAVLAVTDRRSALFQILFAVFDSLEGFVIVMVHCILRREVQDAVKCRVVDRQEEGNGDSGGSFQNGHAQLMTDFEKDVDLACRSVLNKDIAACRTATITGTLKRPSQPEEEKLKLAHAKAPSNFNSLPANVSKLHLHGSPRCPGGPLPDFPNHSLTLKKDRAPKSSFVGDGDIFKKLDSELSRAQEKALDTSYVILPTATATLRPKPPEEPKYSIHIDQMPQTRLIHLSMAPDAGLPARSPPSRQPPGSGPPEPPPTQPPPPPPPPPPPPQQPLPPPPPMEPAPPSLGEPGEPAAHQGPSTGPGTKTENVSTLSVSSLERRKSRYAELDFEKIMHTRKRHQDMFQDLNRKLQHAEKDKDVLGPDSKPEKQQTPNKRPWESLRKAHGPPAWVKKELEPLPPSPLELRSVEWEKSGATIPLVGQDIIDLQTEV

Target ORF information:

RefSeq Version	XM_010811854.2
Organism	Bos taurus(cattle)
Definition	Bos taurus adhesion G protein-coupled receptor B1 (ADGRB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811854.2

ORF Insert Sequence:

ATGAGGGGCC AGGCCGCCGG CCCGGGTGCC CTCTGGATCC TTGGGCTGCT GCTGCTGCTG 
GGGCGCCAGG TGGGCACAGC CGCGGGCACG GCCGCAGGGC CCGGGGCTGA GCCCTGCGCC 
ACGCTGGTGC AGGGCAAGTT CTTCGGCTAC TTCTCGGCAG CCGCGGTGTT CCCGGCCAAC 
GCCTCGCGCT GCTCCTGGAC CCTGCGCAAC CCGGACCCGC GCCGCTACAC GCTCTACATG 
AAGGTGGCCA AGGCGCCCGC CTGCAGCGGC CCCGCCCGCG TCCGCACCTA CCAGTTTGAC 
TCCTTCCTGG AGTCCACGCG CACCTACCTG GGCGTGGAGA GCTTCGATGA GGTGCTGAGG 
CTCTGCGACC CCTCGGCGCC CCTGGCCTTC CTGCAGGCCA GCAAGCAGTT TCTGCAGATG 
CAGCGCCTGC GGCCGCCCCA AGACAACACC CTGAGGACCC GGGACGGGCC CCCCGGGCCC 
AGCGACGACT TCTCCGTGGA GTACCTAGTG GTCGGCAACC GCAACCCCAG CCGGGCCGCC 
TGCCAGATGC TGTGCCGCTG GCTGGACGCC TGTCTGGCTG GCAGCCGCAG CTCGCACCCC 
TGCGGCATCA TGCAGACCCC CTGCGCCTGC CTGGGCGGGG ACACCAGCGA CTCTGCCTCT 
GGCTCCCTGG CCCCCCGTGG GGACGTCTGC CTGAGGGACG GCGTGGCTGG GGGCCCCGAG 
AACTGCCTCA CCAGCCTGAC CCAGGACCGG GGCGGGGATG GCACTCCAGG CGGCTGGAAG 
TTGTGGTCCC TATGGGGCGA GTGCACGCGG GACTGCGGGG GAGGCCTGCA GACGCGGACG 
CGCACCTGCC TGCCCGCGCC GGGTATCCAG GGCGGCGGCT GCGAAGGGGT GCTGGAGGAG 
GGCCGCCTGT GCAACCGCAA GGCCTGCGGC CCCGCCGGGC GCACAAACTC CAGGAGCCAG 
TCCCTGCGGT CCACGGATGC CCGGCGGCGT GAGGAGCTGG GGGACGACCT GCAGCAGTTT 
GGGTTCCCGG CCCCGCAGAC AGGTGACCCC GCGGCGGAGG AGTGGTCCCC GTGGAGCGTG 
TGCTCCAGCA CCTGCGGCGA GGGCTGGCAG ACCCGCACGC GCTTCTGCGT GTCGACCTCC 
TACAGCACGC AGTGCAGCGG GCCCCTGCGG GAGCAGCGGC TCTGCAACAA CTCGGCCGTG 
TGTCCAGTGC ATGGCGCCTG GGACGAGTGG TCGCCTTGGA GCCTATGCTC CAGCACCTGC 
GGTCGCGGCT TCCGGGACCG CACGCGCACC TGCAGGCCGC CCCAGTTTGG AGGCAACCCC 
TGTGAGGGCC CGGAAAAGCA AACCAAGTTC TGCAACATCG CTTTGTGCCC TGTGGATGGA 
AACTGGAACG AGTGGTCCAG CTGGAGCACG TGCTCCGCCA GCTGCTCCCA GGGCCGGCAG 
CAGCGCACAC GCGAGTGCAA CGGGCCTTCG TATGGGGGTG CTGAGTGCCA GGGCCACTGG 
GTAGAGACGC GAGACTGCTT CCTGCAGCAG TGTCCAGTGG ATGGGAAGTG GCAGGCCTGG 
GCGTCCTGGG GCAGCTGCAG CGTCACATGC GGGGGTGGCA CGCAGCGGCG GGAGCGTGCC 
TGCTTGGGGC CCTTCTTTGG GGGAGTGGCC TGCCAGGGCC CCCAGGATGA ATACCGGCAG 
TGTGGCGCCC AGCGGTGTCC CGAGCCCCAC GAGATCTGTG ATGAGGACAA CTTCGGAGCT 
GTGGTCTGGA AGGAGACCCC GGCCGGAGAG GTGGCTGCAG TCCGGTGTCC CCGCAACGCC 
ACAGGCCTCA TCCTGCGACG CTGTGAGCTG GATGAAGAAG GCATTGCCTA CTGGGAGCCC 
CCCACCTATA TCCGCTGTGT GTCGATTGAC TACCGGAACA TCCAAATGAT GACCCGGGAG 
CACCTAGCGA AGGCGCAGCG TGGGCTGCCC GGGGAGGGCG TCTCCGAGGT CATCCAGACC 
CTGGTGGAGA TCTCCCAGGA CGGGACCAGC TACAGCGGGG ACCTGCTGTC CACCATCGAC 
GTCCTAAGGA ACATGACAGA GATCTTCCGC CGGGCGTACT ACAGCCCGAC CCCTGGGGAC 
GTGCAGAACT TTGTGCAGAT CATCAGCAAC TTGCTGGCTG AGGAGAATCG GGACAAGTGG 
GAGGAGGCGC AGCTGATGGG CCCCAATGCC AAGGAGCTCT TCCGGCTAGT GGAGGACTTC 
GTGGACGTCA TCGGCTTCCG CATGAAAGAC TTGCGGGACG CGTACCAGGT GACAGACAAC 
CTGGTCCTCA GCATCCACAA GCTCCCAGCC AGCGGAGCCA CCGACATCAG CTTCCCCATG 
AAGGGCTGGC GGGCCACAGG CGACTGGGCC AAGGTGCCGG AGGACAGGGT CACTGTGTCC 
AAGAGTGTCT TCTCCACGGG GCTGGCAGAG GCTGATGACT CGTCTGTGTT CGTGGTGGGC 
ACTGTGCTTT ACCGAAACCT GGGCAGCTTC CTGGCCCTGC AGAGGAACAC GACTGTCCTG 
AACTCCAAGG TCATCTCGGT GACGGTGAAG CCCCCGCCTC GCTCCCTGCT CACACCCTTG 
GAGATCGAGT TCGCCCACAT GTACAACGGC ACCACCAACC AGACCTGCAT CCTGTGGGAC 
GAGACAGACC CACCCTCCTC CCCCGCCCCC CCGCAGCTCG GGCCCTGGTC GTGGCGCGGC 
TGCCGCACGG TGCCCCTCGA CGCCCTCCGG ACGCGCTGCC TCTGTGACCG GCTCTCCACC 
TTCGCCATCT TAGCCCAGCT CAGCGCCGAC GCGAGCATGG AGAAGGTGGC CACGCCGTCG 
GTGACGCTCA TCGTGGGCTG CGGCGTGTCG TCCCTGACCC TGCTCATGCT GATCATCATC 
TACGTCTCCG TGTGGAGGTA CATCCGCTCG GAGCGCTCCG TCATCCTCAT CAACTTCTGC 
CTGTCCATCA TCTCCTCCAA CGCGCTCATC CTCATCGGGC AGACCCAGAC CCGCAACAAG 
GCCGTGTGCA CGCTGGTGGC CGCCTTCCTG CACTTCTTCT TCCTGTCCTC CTTCTGCTGG 
GTGTTGACCG AGGCCTGGCA GTCCTACATG GCCGTGACTG GCCGCCTGCG GAACCGCCTC 
GTCCGCAAGC GCTTCCTGTG CCTGGGCTGG GGGCTCCCTG CGCTGGTCGT GGCTATTTCC 
GTGGGATTTA CCAAGGCCAG AGGGTACAGC ACCATGAACT ACTGCTGGCT CTCCCTGGAG 
GGGGGACTGC TGTACGCCTT CGTGGGACCG GCCGCGGCCG TCGTGCTGGT GAACATGGTC 
ATCGGGATCC TGGTGTTCAA CAAGCTCGTG TCCAAGGACG GCATCACGGA CAAGAAGCTG 
AAGGAGCGGG CAGGGGCCTC GCTGTGGAGC TCCTGCGTGG TGCTGCCGCT GCTGGCGCTG 
ACCTGGATGT CTGCTGTGCT CGCCGTCACC GACCGCAGGT CCGCCCTCTT CCAGATCCTC 
TTTGCTGTCT TCGACTCGCT GGAGGGCTTC GTCATCGTGA TGGTGCACTG CATCCTACGG 
AGAGAGGTCC AGGACGCTGT GAAGTGCCGT GTGGTGGACC GCCAGGAGGA GGGCAACGGG 
GACTCAGGGG GCTCCTTCCA GAACGGCCAC GCCCAGCTCA TGACCGACTT TGAGAAGGAC 
GTGGATCTGG CCTGTAGATC AGTGCTGAAC AAGGACATTG CAGCTTGCCG CACGGCCACC 
ATCACAGGCA CGCTCAAACG GCCATCGCAG CCGGAGGAGG AGAAGCTGAA GCTGGCCCAT 
GCCAAGGCGC CCTCCAACTT CAACAGCCTG CCGGCCAATG TATCCAAGCT GCACCTGCAC 
GGCTCGCCCC GCTGCCCCGG CGGGCCCCTG CCCGACTTCC CCAACCACTC CCTGACCCTC 
AAGAAAGACA GGGCGCCCAA GTCGTCGTTC GTTGGCGACG GGGACATCTT CAAGAAGCTG 
GACTCGGAGC TGAGCCGGGC CCAGGAGAAA GCCCTGGACA CAAGCTACGT TATCCTGCCC 
ACGGCCACGG CCACGCTGCG GCCCAAGCCC CCGGAGGAGC CCAAGTACAG CATCCACATT 
GACCAGATGC CCCAGACCCG CCTCATCCAC CTCAGCATGG CGCCTGACGC GGGGCTCCCG 
GCCCGCAGCC CGCCCTCCCG CCAGCCCCCC GGCAGCGGGC CCCCCGAGCC GCCCCCCACC 
CAGCCCCCGC CGCCTCCGCC CCCGCCACCC CCACCGCCCC AGCAGCCCCT GCCCCCACCG 
CCCCCCATGG AGCCAGCACC CCCCAGCCTG GGGGAGCCCG GGGAGCCTGC TGCCCACCAG 
GGGCCCAGTA CGGGGCCCGG GACAAAGACA GAGAATGTCT CCACGCTGTC CGTGAGCTCC 
CTGGAGCGGC GGAAATCACG GTATGCGGAA CTGGACTTTG AGAAAATCAT GCACACACGG 
AAACGGCACC AGGACATGTT CCAGGACCTG AACCGGAAGT TACAGCACGC AGAGAAGGAC 
AAGGATGTGC TGGGCCCAGA CAGCAAGCCA GAAAAGCAGC AGACGCCCAA CAAGCGGCCC 
TGGGAGAGCC TCCGGAAAGC GCACGGGCCG CCCGCCTGGG TGAAGAAGGA GCTGGAGCCG 
CTCCCACCGT CCCCACTGGA GCTGCGGAGT GTGGAGTGGG AGAAGTCGGG CGCCACCATC 
CCGCTGGTGG GCCAGGACAT CATCGACCTC CAGACCGAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ADGRB1 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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