MAP3K1 cDNA ORF clone, Bos taurus(cattle)

The following MAP3K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa30607 XM_005221498.4
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa38280 NM_001205906.1
Latest version!
Bos taurus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
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OBa30607 XM_005221498.3 Bos taurus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa30607
    Clone ID Related Accession (Same CDS sequence) XM_005221498.4 , XM_005221498.3
    Accession Version XM_005221498.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221498.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACTCT CAAGGGACTG CACAAGATGG ACGATCGCCC AGAAGAACGA 
    ATGATCAGGG AGAAACTGAA GGCCACCTGT ATGCCAGCCT GGAAGCACGA ATGGCTGGAA
    CGGAGAAACA GGAGAGGCCC TGTGGTGGTA AAACCAATCC CTATTAAAGG AGATGGATCT
    GAAATTAATA ACTTGGCAGC CGAGTCTCAA GGAGAGGGCC CAACGAGCGC TGCTTCCTCC
    ACTCCCAAAG GCCGACGCAG CCCTTCCCCT GGCAGCTCCC CCTCAGGTCG CACAGTGAAA
    TCTGAATCTC CAGGAGTAAG GAGGAAAAGA GTTTCTCCGG TGCCCTTTCA GAGTGGGAGA
    ATCACACCAC CTCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATACAG CCCGGAGGAG
    ACAAACCGCC GAGTGAACAA AGTGATGCGG GCCAGGCTGT ACTTACTGCA GCAGATAGGA
    CCCAACTCCT TCCTGATTGG AGGAGACAGC CCAGACAACA AATACCGGGT GTTTATCGGG
    CCTCAGAACT GCAGCTGTGG GCGTGGAACA TTTTGTATTC ATCTGCTATT TGTTATGCTC
    CGGGTATTTC AGCTAGAACC TTCAGACCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT
    GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCAAGGAT CAAAGCCCCG
    TCTCGTAACA CCATCCAGAA ATTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCGTCA
    TCTAGCACGT CTACATCCAG TTCAGAAAAC AGTATAAAAG ATGAAGAGGA ACAGATGTGT
    CCTATTTGCT TGTTGGGCAT GCTTGATGAA GAAAGTCTTA CAGTGTGTGA AGACGGCTGC
    AGGAACAAGC TGCACCACCA CTGCATGTCC ATTTGGGCAG AAGAATGTAG AAGAAATAGA
    GAACCTTTAA TATGTCCCCT TTGTAGGTCG AAGTGGAGAT CCCACGATTT CTACAGCCAC
    GAGTTGTCCA GCCCTGTGGA TTCCCCTTCT CCCCTGCGAG CTCCACAGCA GCAGACCACC
    CAGCAGCATG CCTTGGCTGG GTCCCAGCGG AGGAATCAGG AGGGCAGTCT TACCCTTACT
    CATTACGGGA CTCAGCAGAT CCCTCCTTCT TACAAAGATT TAGCTGAGCC TTGGATTCAG
    GTATTTGGAA TGGAACTCGT TGGCTGCTTA TTTTCTAGAA ACTGGAATGT AAGAGAGATG
    GCTCTCAGAC GTCTTTCCCA CGACGTTAGT GGGGCTCTGC TGTTGGCAAA TGGGGAGAGC
    ACTGGAAATT CTGGGAGCAG TAGCGGAAGC GGTCCAGGTG CTGGAGCCAC CAGTGGGTCT
    TCCCAGACCA GTATCTCAGG AGATGTGGTG GAGGCATGCT GCAACGTTTT GTCAATGGTC
    TGTGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACGTTGAG AGCCATGCTA
    GTATATACTC CTTGCCATAG TCTGGCAGAA AGAATCAAAC TGCAGAGACT TCTCCGGCCA
    GTTGTAGACA CCATCCTAGT CAAGTGTGCA GACGTCAATA GTCGCACAAG TCAGCTGTCT
    ATATCAACGC TATTGGAATT GTGCAAAGGC CAAGCAGGAG AGTTGGCAGT TGGCAGAGAA
    ATACTTAAAG CTGGATCCAT TGGTATTGGT GGTGTTGATT ATGTCTTAAA TTGTATTCTT
    GGAAACCAAA CTGAATCAAA CAACTGGCAA GAACTCCTGG GCCGGCTCTG TCTCATAGAT
    AGACTGCTGT TGGAATTTCC TGCTGAATTT TACCCTCATA TTGTCAGTAC TGATATTTCA
    CAAGCTGAGC CTGTTGAAGT CAGGTATAAG AAGCTGCTGT CCCTCCTGAC CTTTGCTTTG
    CAGTCCATTG ATAATTCCCA CTCAATGGTT GGTAAACTCT CCCGAAGGAT ATATTTGAGT
    TCTGCAAGAA TGGTTACTGC AGTACCCCAT GTATTTTCAA AACTGTTAGA AATGCTGAAC
    GTTTCTAGTT CCACTCATTT TACCAGGATG CGGCGTCGTT TGATGGCTAT TGCAGATGAG
    GTGGAAATTG CTGAGGCCAT TCAGCTGGGC GTAGAAGACA CTTTGGATGG TCGACATGAC
    TGCTTTTTGC AGGCATCTGC CCCTAACAGC TATCCAGAAG CCATGGACAA TAGTTCCTTT
    GAACGCATGG ACCATTTAGA GAAACCTGGA AAAGGACTGT GTGCTACAAA ATTGAGTGCC
    AGTTCAGAGG ACATTTCTGA CCGACTGGCC AGCATTTCAG TAGGACTTCC TAATTCAACA
    ACAATGGAGC AACCAAAGCC AATGGTTCAA ACAAAGGGCA GACCCCACAG TCAGTGTCTG
    AACGCGTCTC CTGCATCTCC TCATTCCCAG TTAATGTTCC CAGCCTTGTC AACCCCTTCT
    TCATCAACCC CATCTGTACC ATCTGGCACT GGGACAGATC TCTCTAAGCA GAGACCTCAG
    GGGTTTGTTC CCTGCAAAAT GCCTTCTGCA TCTCCTCAAA CACAGCGTAA GTTTTCTCTA
    CAGTTCCAAA GAAACTGTTC TGAAAACAAA GACTCAGATA AACTTTCCCC AATCTTCACT
    CAGTCAAGAC CCCTACCCTC TAGTAATATA CACAGGCCAA AGCCATCTCG ACCTACCCCA
    AGTAATACCA GTAAACAGGG AGATGCCTCA AAAAACAGCA TGACACTTGA TTTGAACAGT
    AGGTCCAAAT GTGATGACAG CTTTGGCAGT AGCAGCAATA GCAGTAATGC TGTTATACCC
    AGTGACGAGA CAGTGTTCAC CCCAGTAGAA GAGAAATGCA GATTAGATGT CAGTACAGAG
    CTCAACTCCA GTATTGAGGA CCTTCTTGAA GCGTCTATGC CTTCAAGTGA CACAACAGTA
    ACTTTTAAGT CAGAAGTTGC TGTGCTCTCT CCTGAAAAAG ATGAAAATGA TGATACCTAC
    AAAGATGATG TGAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAAGAAGAA
    GCTTTAGCGA TTGCCATGGC AATGTCAGCA TCTCAGGATG CCCTCCCCGT AGTTCCTCAA
    CTTCAGGTTG AAAATGGAGA AGATATCATC ATTATTCAAC AAGATACACC AGAGACTCTA
    CCAGGACATA CCAAAGCAAA ACAGCCTTAT AGAGAAGACA GTGAATGGCT GAAAGGTCAG
    CAGATAGGCC TTGGCGCCTT CTCTTCTTGT TATCAGGCTC AAGATGTGGG AACTGGAACT
    TTAATGGCTG TTAAACAGGT GACATATGTC AGAAATACAT CTTCTGAGCA AGAAGAAGTA
    GTAGAAGCAT TAAGAGAAGA GATAAGAATG ATGAGCCATC TGAATCATCC AAACATCATT
    AGGATGTTGG GAGCCACATG CGAGAAGAGC AATTATAATC TCTTCATTGA ATGGATGGCA
    GGGGGATCTG TGGCTCATTT GCTCAGTAAA TATGGAGCCT TCAAGGAGTC GGTAGTTATT
    AACTATACTG AGCAATTACT TCGTGGCCTT TCATATCTCC ATGAAAACCA GATCATTCAC
    AGAGATGTCA AAGGTGCCAA TTTGCTCATT GACAGCACAG GTCAGAGACT GAGAATTGCT
    GACTTTGGAG CTGCAGCCAG GCTGGCATCA AAAGGAACCG GTGCAGGAGA GTTTCAGGGA
    CAGTTACTGG GGACAATTGC ATTTATGGCA CCTGAGGTCC TAAGAGGTCA GCAGTATGGT
    AGGAGCTGTG ATGTATGGAG CGTTGGCTGT GCTATTATAG AAATGGCTTG TGCTAAACCA
    CCTTGGAATG CAGAAAAACA CTCCAATCAT CTTGCTTTGA TATTTAAGAT TGCCAGTGCA
    ACTACTGCTC CATCAATTCC TTCACATTTG TCTCCTGGTT TACGAGATGT GGCTCTTCGT
    TGTTTAGAAC TTCAACCTCA GGACAGACCT CCATCGAGAG AGCTACTGAA GCATCCAGTC
    TTCCGTACTA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221555.1
    CDS190..4227
    Translation

    Target ORF information:

    RefSeq Version XM_005221498.4
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221498.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACTCT CAAGGGACTG CACAAGATGG ACGATCGCCC AGAAGAACGA 
    ATGATCAGGG AGAAACTGAA GGCCACCTGT ATGCCAGCCT GGAAGCACGA ATGGCTGGAA
    CGGAGAAACA GGAGAGGCCC TGTGGTGGTA AAACCAATCC CTATTAAAGG AGATGGATCT
    GAAATTAATA ACTTGGCAGC CGAGTCTCAA GGAGAGGGCC CAACGAGCGC TGCTTCCTCC
    ACTCCCAAAG GCCGACGCAG CCCTTCCCCT GGCAGCTCCC CCTCAGGTCG CACAGTGAAA
    TCTGAATCTC CAGGAGTAAG GAGGAAAAGA GTTTCTCCGG TGCCCTTTCA GAGTGGGAGA
    ATCACACCAC CTCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATACAG CCCGGAGGAG
    ACAAACCGCC GAGTGAACAA AGTGATGCGG GCCAGGCTGT ACTTACTGCA GCAGATAGGA
    CCCAACTCCT TCCTGATTGG AGGAGACAGC CCAGACAACA AATACCGGGT GTTTATCGGG
    CCTCAGAACT GCAGCTGTGG GCGTGGAACA TTTTGTATTC ATCTGCTATT TGTTATGCTC
    CGGGTATTTC AGCTAGAACC TTCAGACCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT
    GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCAAGGAT CAAAGCCCCG
    TCTCGTAACA CCATCCAGAA ATTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCGTCA
    TCTAGCACGT CTACATCCAG TTCAGAAAAC AGTATAAAAG ATGAAGAGGA ACAGATGTGT
    CCTATTTGCT TGTTGGGCAT GCTTGATGAA GAAAGTCTTA CAGTGTGTGA AGACGGCTGC
    AGGAACAAGC TGCACCACCA CTGCATGTCC ATTTGGGCAG AAGAATGTAG AAGAAATAGA
    GAACCTTTAA TATGTCCCCT TTGTAGGTCG AAGTGGAGAT CCCACGATTT CTACAGCCAC
    GAGTTGTCCA GCCCTGTGGA TTCCCCTTCT CCCCTGCGAG CTCCACAGCA GCAGACCACC
    CAGCAGCATG CCTTGGCTGG GTCCCAGCGG AGGAATCAGG AGGGCAGTCT TACCCTTACT
    CATTACGGGA CTCAGCAGAT CCCTCCTTCT TACAAAGATT TAGCTGAGCC TTGGATTCAG
    GTATTTGGAA TGGAACTCGT TGGCTGCTTA TTTTCTAGAA ACTGGAATGT AAGAGAGATG
    GCTCTCAGAC GTCTTTCCCA CGACGTTAGT GGGGCTCTGC TGTTGGCAAA TGGGGAGAGC
    ACTGGAAATT CTGGGAGCAG TAGCGGAAGC GGTCCAGGTG CTGGAGCCAC CAGTGGGTCT
    TCCCAGACCA GTATCTCAGG AGATGTGGTG GAGGCATGCT GCAACGTTTT GTCAATGGTC
    TGTGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACGTTGAG AGCCATGCTA
    GTATATACTC CTTGCCATAG TCTGGCAGAA AGAATCAAAC TGCAGAGACT TCTCCGGCCA
    GTTGTAGACA CCATCCTAGT CAAGTGTGCA GACGTCAATA GTCGCACAAG TCAGCTGTCT
    ATATCAACGC TATTGGAATT GTGCAAAGGC CAAGCAGGAG AGTTGGCAGT TGGCAGAGAA
    ATACTTAAAG CTGGATCCAT TGGTATTGGT GGTGTTGATT ATGTCTTAAA TTGTATTCTT
    GGAAACCAAA CTGAATCAAA CAACTGGCAA GAACTCCTGG GCCGGCTCTG TCTCATAGAT
    AGACTGCTGT TGGAATTTCC TGCTGAATTT TACCCTCATA TTGTCAGTAC TGATATTTCA
    CAAGCTGAGC CTGTTGAAGT CAGGTATAAG AAGCTGCTGT CCCTCCTGAC CTTTGCTTTG
    CAGTCCATTG ATAATTCCCA CTCAATGGTT GGTAAACTCT CCCGAAGGAT ATATTTGAGT
    TCTGCAAGAA TGGTTACTGC AGTACCCCAT GTATTTTCAA AACTGTTAGA AATGCTGAAC
    GTTTCTAGTT CCACTCATTT TACCAGGATG CGGCGTCGTT TGATGGCTAT TGCAGATGAG
    GTGGAAATTG CTGAGGCCAT TCAGCTGGGC GTAGAAGACA CTTTGGATGG TCGACATGAC
    TGCTTTTTGC AGGCATCTGC CCCTAACAGC TATCCAGAAG CCATGGACAA TAGTTCCTTT
    GAACGCATGG ACCATTTAGA GAAACCTGGA AAAGGACTGT GTGCTACAAA ATTGAGTGCC
    AGTTCAGAGG ACATTTCTGA CCGACTGGCC AGCATTTCAG TAGGACTTCC TAATTCAACA
    ACAATGGAGC AACCAAAGCC AATGGTTCAA ACAAAGGGCA GACCCCACAG TCAGTGTCTG
    AACGCGTCTC CTGCATCTCC TCATTCCCAG TTAATGTTCC CAGCCTTGTC AACCCCTTCT
    TCATCAACCC CATCTGTACC ATCTGGCACT GGGACAGATC TCTCTAAGCA GAGACCTCAG
    GGGTTTGTTC CCTGCAAAAT GCCTTCTGCA TCTCCTCAAA CACAGCGTAA GTTTTCTCTA
    CAGTTCCAAA GAAACTGTTC TGAAAACAAA GACTCAGATA AACTTTCCCC AATCTTCACT
    CAGTCAAGAC CCCTACCCTC TAGTAATATA CACAGGCCAA AGCCATCTCG ACCTACCCCA
    AGTAATACCA GTAAACAGGG AGATGCCTCA AAAAACAGCA TGACACTTGA TTTGAACAGT
    AGGTCCAAAT GTGATGACAG CTTTGGCAGT AGCAGCAATA GCAGTAATGC TGTTATACCC
    AGTGACGAGA CAGTGTTCAC CCCAGTAGAA GAGAAATGCA GATTAGATGT CAGTACAGAG
    CTCAACTCCA GTATTGAGGA CCTTCTTGAA GCGTCTATGC CTTCAAGTGA CACAACAGTA
    ACTTTTAAGT CAGAAGTTGC TGTGCTCTCT CCTGAAAAAG ATGAAAATGA TGATACCTAC
    AAAGATGATG TGAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAAGAAGAA
    GCTTTAGCGA TTGCCATGGC AATGTCAGCA TCTCAGGATG CCCTCCCCGT AGTTCCTCAA
    CTTCAGGTTG AAAATGGAGA AGATATCATC ATTATTCAAC AAGATACACC AGAGACTCTA
    CCAGGACATA CCAAAGCAAA ACAGCCTTAT AGAGAAGACA GTGAATGGCT GAAAGGTCAG
    CAGATAGGCC TTGGCGCCTT CTCTTCTTGT TATCAGGCTC AAGATGTGGG AACTGGAACT
    TTAATGGCTG TTAAACAGGT GACATATGTC AGAAATACAT CTTCTGAGCA AGAAGAAGTA
    GTAGAAGCAT TAAGAGAAGA GATAAGAATG ATGAGCCATC TGAATCATCC AAACATCATT
    AGGATGTTGG GAGCCACATG CGAGAAGAGC AATTATAATC TCTTCATTGA ATGGATGGCA
    GGGGGATCTG TGGCTCATTT GCTCAGTAAA TATGGAGCCT TCAAGGAGTC GGTAGTTATT
    AACTATACTG AGCAATTACT TCGTGGCCTT TCATATCTCC ATGAAAACCA GATCATTCAC
    AGAGATGTCA AAGGTGCCAA TTTGCTCATT GACAGCACAG GTCAGAGACT GAGAATTGCT
    GACTTTGGAG CTGCAGCCAG GCTGGCATCA AAAGGAACCG GTGCAGGAGA GTTTCAGGGA
    CAGTTACTGG GGACAATTGC ATTTATGGCA CCTGAGGTCC TAAGAGGTCA GCAGTATGGT
    AGGAGCTGTG ATGTATGGAG CGTTGGCTGT GCTATTATAG AAATGGCTTG TGCTAAACCA
    CCTTGGAATG CAGAAAAACA CTCCAATCAT CTTGCTTTGA TATTTAAGAT TGCCAGTGCA
    ACTACTGCTC CATCAATTCC TTCACATTTG TCTCCTGGTT TACGAGATGT GGCTCTTCGT
    TGTTTAGAAC TTCAACCTCA GGACAGACCT CCATCGAGAG AGCTACTGAA GCATCCAGTC
    TTCCGTACTA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30607
    Clone ID Related Accession (Same CDS sequence) XM_005221498.4 , XM_005221498.3
    Accession Version XM_005221498.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104511.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACTCT CAAGGGACTG CACAAGATGG ACGATCGCCC AGAAGAACGA 
    ATGATCAGGG AGAAACTGAA GGCCACCTGT ATGCCAGCCT GGAAGCACGA ATGGCTGGAA
    CGGAGAAACA GGAGAGGCCC TGTGGTGGTA AAACCAATCC CTATTAAAGG AGATGGATCT
    GAAATTAATA ACTTGGCAGC CGAGTCTCAA GGAGAGGGCC CAACGAGCGC TGCTTCCTCC
    ACTCCCAAAG GCCGACGCAG CCCTTCCCCT GGCAGCTCCC CCTCAGGTCG CACAGTGAAA
    TCTGAATCTC CAGGAGTAAG GAGGAAAAGA GTTTCTCCGG TGCCCTTTCA GAGTGGGAGA
    ATCACACCAC CTCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATACAG CCCGGAGGAG
    ACAAACCGCC GAGTGAACAA AGTGATGCGG GCCAGGCTGT ACTTACTGCA GCAGATAGGA
    CCCAACTCCT TCCTGATTGG AGGAGACAGC CCAGACAACA AATACCGGGT GTTTATCGGG
    CCTCAGAACT GCAGCTGTGG GCGTGGAACA TTTTGTATTC ATCTGCTATT TGTTATGCTC
    CGGGTATTTC AGCTAGAACC TTCAGACCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT
    GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCAAGGAT CAAAGCCCCG
    TCTCGTAACA CCATCCAGAA ATTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCGTCA
    TCTAGCACGT CTACATCCAG TTCAGAAAAC AGTATAAAAG ATGAAGAGGA ACAGATGTGT
    CCTATTTGCT TGTTGGGCAT GCTTGATGAA GAAAGTCTTA CAGTGTGTGA AGACGGCTGC
    AGGAACAAGC TGCACCACCA CTGCATGTCC ATTTGGGCAG AAGAATGTAG AAGAAATAGA
    GAACCTTTAA TATGTCCCCT TTGTAGGTCG AAGTGGAGAT CCCACGATTT CTACAGCCAC
    GAGTTGTCCA GCCCTGTGGA TTCCCCTTCT CCCCTGCGAG CTCCACAGCA GCAGACCACC
    CAGCAGCATG CCTTGGCTGG GTCCCAGCGG AGGAATCAGG AGGGCAGTCT TACCCTTACT
    CATTACGGGA CTCAGCAGAT CCCTCCTTCT TACAAAGATT TAGCTGAGCC TTGGATTCAG
    GTATTTGGAA TGGAACTCGT TGGCTGCTTA TTTTCTAGAA ACTGGAATGT AAGAGAGATG
    GCTCTCAGAC GTCTTTCCCA CGACGTTAGT GGGGCTCTGC TGTTGGCAAA TGGGGAGAGC
    ACTGGAAATT CTGGGAGCAG TAGCGGAAGC GGTCCAGGTG CTGGAGCCAC CAGTGGGTCT
    TCCCAGACCA GTATCTCAGG AGATGTGGTG GAGGCATGCT GCAACGTTTT GTCAATGGTC
    TGTGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACGTTGAG AGCCATGCTA
    GTATATACTC CTTGCCATAG TCTGGCAGAA AGAATCAAAC TGCAGAGACT TCTCCGGCCA
    GTTGTAGACA CCATCCTAGT CAAGTGTGCA GACGTCAATA GTCGCACAAG TCAGCTGTCT
    ATATCAACGC TATTGGAATT GTGCAAAGGC CAAGCAGGAG AGTTGGCAGT TGGCAGAGAA
    ATACTTAAAG CTGGATCCAT TGGTATTGGT GGTGTTGATT ATGTCTTAAA TTGTATTCTT
    GGAAACCAAA CTGAATCAAA CAACTGGCAA GAACTCCTGG GCCGGCTCTG TCTCATAGAT
    AGACTGCTGT TGGAATTTCC TGCTGAATTT TACCCTCATA TTGTCAGTAC TGATATTTCA
    CAAGCTGAGC CTGTTGAAGT CAGGTATAAG AAGCTGCTGT CCCTCCTGAC CTTTGCTTTG
    CAGTCCATTG ATAATTCCCA CTCAATGGTT GGTAAACTCT CCCGAAGGAT ATATTTGAGT
    TCTGCAAGAA TGGTTACTGC AGTACCCCAT GTATTTTCAA AACTGTTAGA AATGCTGAAC
    GTTTCTAGTT CCACTCATTT TACCAGGATG CGGCGTCGTT TGATGGCTAT TGCAGATGAG
    GTGGAAATTG CTGAGGCCAT TCAGCTGGGC GTAGAAGACA CTTTGGATGG TCGACATGAC
    TGCTTTTTGC AGGCATCTGC CCCTAACAGC TATCCAGAAG CCATGGACAA TAGTTCCTTT
    GAACGCATGG ACCATTTAGA GAAACCTGGA AAAGGACTGT GTGCTACAAA ATTGAGTGCC
    AGTTCAGAGG ACATTTCTGA CCGACTGGCC AGCATTTCAG TAGGACTTCC TAATTCAACA
    ACAATGGAGC AACCAAAGCC AATGGTTCAA ACAAAGGGCA GACCCCACAG TCAGTGTCTG
    AACGCGTCTC CTGCATCTCC TCATTCCCAG TTAATGTTCC CAGCCTTGTC AACCCCTTCT
    TCATCAACCC CATCTGTACC ATCTGGCACT GGGACAGATC TCTCTAAGCA GAGACCTCAG
    GGGTTTGTTC CCTGCAAAAT GCCTTCTGCA TCTCCTCAAA CACAGCGTAA GTTTTCTCTA
    CAGTTCCAAA GAAACTGTTC TGAAAACAAA GACTCAGATA AACTTTCCCC AATCTTCACT
    CAGTCAAGAC CCCTACCCTC TAGTAATATA CACAGGCCAA AGCCATCTCG ACCTACCCCA
    AGTAATACCA GTAAACAGGG AGATGCCTCA AAAAACAGCA TGACACTTGA TTTGAACAGT
    AGGTCCAAAT GTGATGACAG CTTTGGCAGT AGCAGCAATA GCAGTAATGC TGTTATACCC
    AGTGACGAGA CAGTGTTCAC CCCAGTAGAA GAGAAATGCA GATTAGATGT CAGTACAGAG
    CTCAACTCCA GTATTGAGGA CCTTCTTGAA GCGTCTATGC CTTCAAGTGA CACAACAGTA
    ACTTTTAAGT CAGAAGTTGC TGTGCTCTCT CCTGAAAAAG ATGAAAATGA TGATACCTAC
    AAAGATGATG TGAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAAGAAGAA
    GCTTTAGCGA TTGCCATGGC AATGTCAGCA TCTCAGGATG CCCTCCCCGT AGTTCCTCAA
    CTTCAGGTTG AAAATGGAGA AGATATCATC ATTATTCAAC AAGATACACC AGAGACTCTA
    CCAGGACATA CCAAAGCAAA ACAGCCTTAT AGAGAAGACA GTGAATGGCT GAAAGGTCAG
    CAGATAGGCC TTGGCGCCTT CTCTTCTTGT TATCAGGCTC AAGATGTGGG AACTGGAACT
    TTAATGGCTG TTAAACAGGT GACATATGTC AGAAATACAT CTTCTGAGCA AGAAGAAGTA
    GTAGAAGCAT TAAGAGAAGA GATAAGAATG ATGAGCCATC TGAATCATCC AAACATCATT
    AGGATGTTGG GAGCCACATG CGAGAAGAGC AATTATAATC TCTTCATTGA ATGGATGGCA
    GGGGGATCTG TGGCTCATTT GCTCAGTAAA TATGGAGCCT TCAAGGAGTC GGTAGTTATT
    AACTATACTG AGCAATTACT TCGTGGCCTT TCATATCTCC ATGAAAACCA GATCATTCAC
    AGAGATGTCA AAGGTGCCAA TTTGCTCATT GACAGCACAG GTCAGAGACT GAGAATTGCT
    GACTTTGGAG CTGCAGCCAG GCTGGCATCA AAAGGAACCG GTGCAGGAGA GTTTCAGGGA
    CAGTTACTGG GGACAATTGC ATTTATGGCA CCTGAGGTCC TAAGAGGTCA GCAGTATGGT
    AGGAGCTGTG ATGTATGGAG CGTTGGCTGT GCTATTATAG AAATGGCTTG TGCTAAACCA
    CCTTGGAATG CAGAAAAACA CTCCAATCAT CTTGCTTTGA TATTTAAGAT TGCCAGTGCA
    ACTACTGCTC CATCAATTCC TTCACATTTG TCTCCTGGTT TACGAGATGT GGCTCTTCGT
    TGTTTAGAAC TTCAACCTCA GGACAGACCT CCATCGAGAG AGCTACTGAA GCATCCAGTC
    TTCCGTACTA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221555.1
    CDS95..4132
    Translation

    Target ORF information:

    RefSeq Version XM_005221498.3
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221498.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGAATA AAGAAACTCT CAAGGGACTG CACAAGATGG ACGATCGCCC AGAAGAACGA 
    ATGATCAGGG AGAAACTGAA GGCCACCTGT ATGCCAGCCT GGAAGCACGA ATGGCTGGAA
    CGGAGAAACA GGAGAGGCCC TGTGGTGGTA AAACCAATCC CTATTAAAGG AGATGGATCT
    GAAATTAATA ACTTGGCAGC CGAGTCTCAA GGAGAGGGCC CAACGAGCGC TGCTTCCTCC
    ACTCCCAAAG GCCGACGCAG CCCTTCCCCT GGCAGCTCCC CCTCAGGTCG CACAGTGAAA
    TCTGAATCTC CAGGAGTAAG GAGGAAAAGA GTTTCTCCGG TGCCCTTTCA GAGTGGGAGA
    ATCACACCAC CTCGAAGAGC CCCTTCACCA GATGGCTTCT CACCATACAG CCCGGAGGAG
    ACAAACCGCC GAGTGAACAA AGTGATGCGG GCCAGGCTGT ACTTACTGCA GCAGATAGGA
    CCCAACTCCT TCCTGATTGG AGGAGACAGC CCAGACAACA AATACCGGGT GTTTATCGGG
    CCTCAGAACT GCAGCTGTGG GCGTGGAACA TTTTGTATTC ATCTGCTATT TGTTATGCTC
    CGGGTATTTC AGCTAGAACC TTCAGACCCA ATGTTATGGA GAAAAACTTT AAAGAATTTT
    GAGGTTGAGA GTTTGTTCCA GAAATACCAC AGTAGGCGTA GCTCAAGGAT CAAAGCCCCG
    TCTCGTAACA CCATCCAGAA ATTTGTTTCA CGCATGTCAA ATTCTCATAC ATTGTCGTCA
    TCTAGCACGT CTACATCCAG TTCAGAAAAC AGTATAAAAG ATGAAGAGGA ACAGATGTGT
    CCTATTTGCT TGTTGGGCAT GCTTGATGAA GAAAGTCTTA CAGTGTGTGA AGACGGCTGC
    AGGAACAAGC TGCACCACCA CTGCATGTCC ATTTGGGCAG AAGAATGTAG AAGAAATAGA
    GAACCTTTAA TATGTCCCCT TTGTAGGTCG AAGTGGAGAT CCCACGATTT CTACAGCCAC
    GAGTTGTCCA GCCCTGTGGA TTCCCCTTCT CCCCTGCGAG CTCCACAGCA GCAGACCACC
    CAGCAGCATG CCTTGGCTGG GTCCCAGCGG AGGAATCAGG AGGGCAGTCT TACCCTTACT
    CATTACGGGA CTCAGCAGAT CCCTCCTTCT TACAAAGATT TAGCTGAGCC TTGGATTCAG
    GTATTTGGAA TGGAACTCGT TGGCTGCTTA TTTTCTAGAA ACTGGAATGT AAGAGAGATG
    GCTCTCAGAC GTCTTTCCCA CGACGTTAGT GGGGCTCTGC TGTTGGCAAA TGGGGAGAGC
    ACTGGAAATT CTGGGAGCAG TAGCGGAAGC GGTCCAGGTG CTGGAGCCAC CAGTGGGTCT
    TCCCAGACCA GTATCTCAGG AGATGTGGTG GAGGCATGCT GCAACGTTTT GTCAATGGTC
    TGTGCTGACC CTGTCTACAA AGTGTACGTT GCTGCTTTAA AAACGTTGAG AGCCATGCTA
    GTATATACTC CTTGCCATAG TCTGGCAGAA AGAATCAAAC TGCAGAGACT TCTCCGGCCA
    GTTGTAGACA CCATCCTAGT CAAGTGTGCA GACGTCAATA GTCGCACAAG TCAGCTGTCT
    ATATCAACGC TATTGGAATT GTGCAAAGGC CAAGCAGGAG AGTTGGCAGT TGGCAGAGAA
    ATACTTAAAG CTGGATCCAT TGGTATTGGT GGTGTTGATT ATGTCTTAAA TTGTATTCTT
    GGAAACCAAA CTGAATCAAA CAACTGGCAA GAACTCCTGG GCCGGCTCTG TCTCATAGAT
    AGACTGCTGT TGGAATTTCC TGCTGAATTT TACCCTCATA TTGTCAGTAC TGATATTTCA
    CAAGCTGAGC CTGTTGAAGT CAGGTATAAG AAGCTGCTGT CCCTCCTGAC CTTTGCTTTG
    CAGTCCATTG ATAATTCCCA CTCAATGGTT GGTAAACTCT CCCGAAGGAT ATATTTGAGT
    TCTGCAAGAA TGGTTACTGC AGTACCCCAT GTATTTTCAA AACTGTTAGA AATGCTGAAC
    GTTTCTAGTT CCACTCATTT TACCAGGATG CGGCGTCGTT TGATGGCTAT TGCAGATGAG
    GTGGAAATTG CTGAGGCCAT TCAGCTGGGC GTAGAAGACA CTTTGGATGG TCGACATGAC
    TGCTTTTTGC AGGCATCTGC CCCTAACAGC TATCCAGAAG CCATGGACAA TAGTTCCTTT
    GAACGCATGG ACCATTTAGA GAAACCTGGA AAAGGACTGT GTGCTACAAA ATTGAGTGCC
    AGTTCAGAGG ACATTTCTGA CCGACTGGCC AGCATTTCAG TAGGACTTCC TAATTCAACA
    ACAATGGAGC AACCAAAGCC AATGGTTCAA ACAAAGGGCA GACCCCACAG TCAGTGTCTG
    AACGCGTCTC CTGCATCTCC TCATTCCCAG TTAATGTTCC CAGCCTTGTC AACCCCTTCT
    TCATCAACCC CATCTGTACC ATCTGGCACT GGGACAGATC TCTCTAAGCA GAGACCTCAG
    GGGTTTGTTC CCTGCAAAAT GCCTTCTGCA TCTCCTCAAA CACAGCGTAA GTTTTCTCTA
    CAGTTCCAAA GAAACTGTTC TGAAAACAAA GACTCAGATA AACTTTCCCC AATCTTCACT
    CAGTCAAGAC CCCTACCCTC TAGTAATATA CACAGGCCAA AGCCATCTCG ACCTACCCCA
    AGTAATACCA GTAAACAGGG AGATGCCTCA AAAAACAGCA TGACACTTGA TTTGAACAGT
    AGGTCCAAAT GTGATGACAG CTTTGGCAGT AGCAGCAATA GCAGTAATGC TGTTATACCC
    AGTGACGAGA CAGTGTTCAC CCCAGTAGAA GAGAAATGCA GATTAGATGT CAGTACAGAG
    CTCAACTCCA GTATTGAGGA CCTTCTTGAA GCGTCTATGC CTTCAAGTGA CACAACAGTA
    ACTTTTAAGT CAGAAGTTGC TGTGCTCTCT CCTGAAAAAG ATGAAAATGA TGATACCTAC
    AAAGATGATG TGAATCATAA TCAAAAGTGC AAAGAAAAGA TGGAAGCTGA AGAAGAAGAA
    GCTTTAGCGA TTGCCATGGC AATGTCAGCA TCTCAGGATG CCCTCCCCGT AGTTCCTCAA
    CTTCAGGTTG AAAATGGAGA AGATATCATC ATTATTCAAC AAGATACACC AGAGACTCTA
    CCAGGACATA CCAAAGCAAA ACAGCCTTAT AGAGAAGACA GTGAATGGCT GAAAGGTCAG
    CAGATAGGCC TTGGCGCCTT CTCTTCTTGT TATCAGGCTC AAGATGTGGG AACTGGAACT
    TTAATGGCTG TTAAACAGGT GACATATGTC AGAAATACAT CTTCTGAGCA AGAAGAAGTA
    GTAGAAGCAT TAAGAGAAGA GATAAGAATG ATGAGCCATC TGAATCATCC AAACATCATT
    AGGATGTTGG GAGCCACATG CGAGAAGAGC AATTATAATC TCTTCATTGA ATGGATGGCA
    GGGGGATCTG TGGCTCATTT GCTCAGTAAA TATGGAGCCT TCAAGGAGTC GGTAGTTATT
    AACTATACTG AGCAATTACT TCGTGGCCTT TCATATCTCC ATGAAAACCA GATCATTCAC
    AGAGATGTCA AAGGTGCCAA TTTGCTCATT GACAGCACAG GTCAGAGACT GAGAATTGCT
    GACTTTGGAG CTGCAGCCAG GCTGGCATCA AAAGGAACCG GTGCAGGAGA GTTTCAGGGA
    CAGTTACTGG GGACAATTGC ATTTATGGCA CCTGAGGTCC TAAGAGGTCA GCAGTATGGT
    AGGAGCTGTG ATGTATGGAG CGTTGGCTGT GCTATTATAG AAATGGCTTG TGCTAAACCA
    CCTTGGAATG CAGAAAAACA CTCCAATCAT CTTGCTTTGA TATTTAAGAT TGCCAGTGCA
    ACTACTGCTC CATCAATTCC TTCACATTTG TCTCCTGGTT TACGAGATGT GGCTCTTCGT
    TGTTTAGAAC TTCAACCTCA GGACAGACCT CCATCGAGAG AGCTACTGAA GCATCCAGTC
    TTCCGTACTA CGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38280
    Clone ID Related Accession (Same CDS sequence) NM_001205906.1
    Accession Version NM_001205906.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000020.1. On Apr 20, 2011 this sequence version replaced XM_602275.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCCTCGGGAT TCCCGGGCGC CGGGGCGGCG 
    AGCCCCGAAG CGGGCGGCGG CAGAGGGGCC CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC
    GCGGGGCTGC TGCGGGAGGC GGGCAGTGGG GGCCGTGAGC GGGCGGACTG GAGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CCGAGCCGCC GCTTTTCCTC
    GCCGCCTCGC CGCCGTCCTC CTCCACTTCC CCGTCGCCGG AGCCCCCGGA CGCGGCGGCG
    GGCGGGAGCG GTTTCCAGCC TGTGGCGGTG CCGCCGCCCC ACGGAGCCGC GAGCCGCGGT
    GGCGCCCACC CTGCCGAGCC GGCGGCCGCA CCGGACAGCG GCGCCCAGAG CCCCGCGGGG
    GCCGAGCCGG GGGAGAAGCG GACGCCCGCC GCCGAGCCGA CTGCGGCGGC CCCGGCTGGT
    CGTGAGATGG AGAATAAAGA AACTCTCAAG GGACTGCACA AGATGGACGA TCGCCCAGAA
    GAACGAATGA TCAGGGAGAA ACTGAAGGCC ACCTGTATGC CAGCCTGGAA GCACGAATGG
    CTGGAACGGA GAAACAGGAG AGGCCCTGTG GTGGTAAAAC CAATCCCTAT TAAAGGAGAT
    GGATCTGAAA TTAATAACTT GGCAGCCGAG TCTCAAGGAG AGGGCCCAAC GAGCGCTGCT
    TCCTCCACTC CCAAAGGCCG ACGCAGCCCT TCCCCTGGCA GCTCCCCCTC AGGTCGCACA
    GTGAAATCTG AATCTCCAGG AGTAAGGAGG AAAAGAGTTT CTCCGGTGCC CTTTCAGAGT
    GGGAGAATCA CACCACCTCG AAGAGCCCCT TCACCAGATG GCTTCTCACC ATACAGCCCG
    GAGGAGACAA ACCGCCGAGT GAACAAAGTG ATGCGGGCCA GGCTGTACTT ACTGCAGCAG
    ATAGGACCCA ACTCCTTCCT GATTGGAGGA GACAGCCCAG ACAACAAATA CCGGGTGTTT
    ATCGGGCCTC AGAACTGCAG CTGTGGGCGT GGAACATTTT GTATTCATCT GCTATTTGTT
    ATGCTCCGGG TATTTCAGCT AGAACCTTCA GACCCAATGT TATGGAGAAA AACTTTAAAG
    AATTTTGAGG TTGAGAGTTT GTTCCAGAAA TACCACAGTA GGCGTAGCTC AAGGATCAAA
    GCCCCGTCTC GTAACACCAT CCAGAAATTT GTTTCACGCA TGTCAAATTC TCATACATTG
    TCGTCATCTA GCACGTCTAC ATCCAGTTCA GAAAACAGTA TAAAAGATGA AGAGGAACAG
    ATGTGTCCTA TTTGCTTGTT GGGCATGCTT GATGAAGAAA GTCTTACAGT GTGTGAAGAC
    GGCTGCAGGA ACAAGCTGCA CCACCACTGC ATGTCCATTT GGGCAGAAGA ATGTAGAAGA
    AATAGAGAAC CTTTAATATG TCCCCTTTGT AGGTCGAAGT GGAGATCCCA CGATTTCTAC
    AGCCACGAGT TGTCCAGCCC TGTGGATTCC CCTTCTCCCC TGCGAGCTCC ACAGCAGCAG
    ACCACCCAGC AGCATGCCTT GGCTGGGTCC CAGCGGAGGA ATCAGGAGGG CAGTCTTACC
    CTTACTCATT ACGGGACTCA GCAGATCCCT CCTTCTTACA AAGATTTAGC TGAGCCTTGG
    ATTCAGGTAT TTGGAATGGA ACTCGTTGGC TGCTTATTTT CTAGAAACTG GAATGTAAGA
    GAGATGGCTC TCAGACGTCT TTCCCACGAC GTTAGTGGGG CTCTGCTGTT GGCAAATGGG
    GAGAGCACTG GAAATTCTGG GAGCAGTAGC GGAAGCGGTC CAGGTGCTGG AGCCACCAGT
    GGGTCTTCCC AGACCAGTAT CTCAGGAGAT GTGGTGGAGG CATGCTGCAA CGTTTTGTCA
    ATGGTCTGTG CTGACCCTGT CTACAAAGTG TACGTTGCTG CTTTAAAAAC GTTGAGAGCC
    ATGCTAGTAT ATACTCCTTG CCATAGTCTG GCAGAAAGAA TCAAACTGCA GAGACTTCTC
    CGGCCAGTTG TAGACACCAT CCTAGTCAAG TGTGCAGACG TCAATAGTCG CACAAGTCAG
    CTGTCTATAT CAACGCTATT GGAATTGTGC AAAGGCCAAG CAGGAGAGTT GGCAGTTGGC
    AGAGAAATAC TTAAAGCTGG ATCCATTGGT ATTGGTGGTG TTGATTATGT CTTAAATTGT
    ATTCTTGGAA ACCAAACTGA ATCAAACAAC TGGCAAGAAC TCCTGGGCCG GCTCTGTCTC
    ATAGATAGAC TGCTGTTGGA ATTTCCTGCT GAATTTTACC CTCATATTGT CAGTACTGAT
    ATTTCACAAG CTGAGCCTGT TGAAGTCAGG TATAAGAAGC TGCTGTCCCT CCTGACCTTT
    GCTTTGCAGT CCATTGATAA TTCCCACTCA ATGGTTGGTA AACTCTCCCG AAGGATATAT
    TTGAGTTCTG CAAGAATGGT TACTGCAGTA CCCCATGTAT TTTCAAAACT GTTAGAAATG
    CTGAACGTTT CTAGTTCCAC TCATTTTACC AGGATGCGGC GTCGTTTGAT GGCTATTGCA
    GATGAGGTGG AAATTGCTGA GGCCATTCAG CTGGGCGTAG AAGACACTTT GGATGGTCGA
    CATGACTGCT TTTTGCAGGC ATCTGCCCCT AACAGCTATC CAGAAGCCAT GGACAATAGT
    TCCTTTGAAC GCATGGACCA TTTAGAGAAA CCTGGAAAAG GACTGTGTGC TACAAAATTG
    AGTGCCAGTT CAGAGGACAT TTCTGACCGA CTGGCCAGCA TTTCAGTAGG ACTTCCTAAT
    TCAACAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AGGGCAGACC CCACAGTCAG
    TGTCTGAACG CGTCTCCTGC ATCTCCTCAT TCCCAGTTAA TGTTCCCAGC CTTGTCAACC
    CCTTCTTCAT CAACCCCATC TGTACCATCT GGCACTGGGA CAGATCTCTC TAAGCAGAGA
    CCTCAGGGGT TTGTTCCCTG CAAAATGCCT TCTGCATCTC CTCAAACACA GCGTAAGTTT
    TCTCTACAGT TCCAAAGAAA CTGTTCTGAA AACAAAGACT CAGATAAACT TTCCCCAATC
    TTCACTCAGT CAAGACCCCT ACCCTCTAGT AATATACACA GGCCAAAGCC ATCTCGACCT
    ACCCCAAGTA ATACCAGTAA ACAGGGAGAT GCCTCAAAAA ACAGCATGAC ACTTGATTTG
    AACAGTAGGT CCAAATGTGA TGACAGCTTT GGCAGTAGCA GCAATAGCAG TAATGCTGTT
    ATACCCAGTG ACGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGATT AGATGTCAGT
    ACAGAGCTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCGT CTATGCCTTC AAGTGACACA
    ACAGTAACTT TTAAGTCAGA AGTTGCTGTG CTCTCTCCTG AAAAAGATGA AAATGATGAT
    ACCTACAAAG ATGATGTGAA TCATAATCAA AAGTGCAAAG AAAAGATGGA AGCTGAAGAA
    GAAGAAGCTT TAGCGATTGC CATGGCAATG TCAGCATCTC AGGATGCCCT CCCCGTAGTT
    CCTCAACTTC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAAGA TACACCAGAG
    ACTCTACCAG GACATACCAA AGCAAAACAG CCTTATAGAG AAGACAGTGA ATGGCTGAAA
    GGTCAGCAGA TAGGCCTTGG CGCCTTCTCT TCTTGTTATC AGGCTCAAGA TGTGGGAACT
    GGAACTTTAA TGGCTGTTAA ACAGGTGACA TATGTCAGAA ATACATCTTC TGAGCAAGAA
    GAAGTAGTAG AAGCATTAAG AGAAGAGATA AGAATGATGA GCCATCTGAA TCATCCAAAC
    ATCATTAGGA TGTTGGGAGC CACATGCGAG AAGAGCAATT ATAATCTCTT CATTGAATGG
    ATGGCAGGGG GATCTGTGGC TCATTTGCTC AGTAAATATG GAGCCTTCAA GGAGTCGGTA
    GTTATTAACT ATACTGAGCA ATTACTTCGT GGCCTTTCAT ATCTCCATGA AAACCAGATC
    ATTCACAGAG ATGTCAAAGG TGCCAATTTG CTCATTGACA GCACAGGTCA GAGACTGAGA
    ATTGCTGACT TTGGAGCTGC AGCCAGGCTG GCATCAAAAG GAACCGGTGC AGGAGAGTTT
    CAGGGACAGT TACTGGGGAC AATTGCATTT ATGGCACCTG AGGTCCTAAG AGGTCAGCAG
    TATGGTAGGA GCTGTGATGT ATGGAGCGTT GGCTGTGCTA TTATAGAAAT GGCTTGTGCT
    AAACCACCTT GGAATGCAGA AAAACACTCC AATCATCTTG CTTTGATATT TAAGATTGCC
    AGTGCAACTA CTGCTCCATC AATTCCTTCA CATTTGTCTC CTGGTTTACG AGATGTGGCT
    CTTCGTTGTT TAGAACTTCA ACCTCAGGAC AGACCTCCAT CGAGAGAGCT ACTGAAGCAT
    CCAGTCTTCC GTACTACGTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192835.1
    CDS1..4524
    Translation

    Target ORF information:

    RefSeq Version NM_001205906.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase 1 (MAP3K1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205906.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCCTCGGGAT TCCCGGGCGC CGGGGCGGCG 
    AGCCCCGAAG CGGGCGGCGG CAGAGGGGCC CTCAAGGCGA GCAGCGCGCC CGCGGCAGCC
    GCGGGGCTGC TGCGGGAGGC GGGCAGTGGG GGCCGTGAGC GGGCGGACTG GAGGCGGCGG
    CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CCGAGCCGCC GCTTTTCCTC
    GCCGCCTCGC CGCCGTCCTC CTCCACTTCC CCGTCGCCGG AGCCCCCGGA CGCGGCGGCG
    GGCGGGAGCG GTTTCCAGCC TGTGGCGGTG CCGCCGCCCC ACGGAGCCGC GAGCCGCGGT
    GGCGCCCACC CTGCCGAGCC GGCGGCCGCA CCGGACAGCG GCGCCCAGAG CCCCGCGGGG
    GCCGAGCCGG GGGAGAAGCG GACGCCCGCC GCCGAGCCGA CTGCGGCGGC CCCGGCTGGT
    CGTGAGATGG AGAATAAAGA AACTCTCAAG GGACTGCACA AGATGGACGA TCGCCCAGAA
    GAACGAATGA TCAGGGAGAA ACTGAAGGCC ACCTGTATGC CAGCCTGGAA GCACGAATGG
    CTGGAACGGA GAAACAGGAG AGGCCCTGTG GTGGTAAAAC CAATCCCTAT TAAAGGAGAT
    GGATCTGAAA TTAATAACTT GGCAGCCGAG TCTCAAGGAG AGGGCCCAAC GAGCGCTGCT
    TCCTCCACTC CCAAAGGCCG ACGCAGCCCT TCCCCTGGCA GCTCCCCCTC AGGTCGCACA
    GTGAAATCTG AATCTCCAGG AGTAAGGAGG AAAAGAGTTT CTCCGGTGCC CTTTCAGAGT
    GGGAGAATCA CACCACCTCG AAGAGCCCCT TCACCAGATG GCTTCTCACC ATACAGCCCG
    GAGGAGACAA ACCGCCGAGT GAACAAAGTG ATGCGGGCCA GGCTGTACTT ACTGCAGCAG
    ATAGGACCCA ACTCCTTCCT GATTGGAGGA GACAGCCCAG ACAACAAATA CCGGGTGTTT
    ATCGGGCCTC AGAACTGCAG CTGTGGGCGT GGAACATTTT GTATTCATCT GCTATTTGTT
    ATGCTCCGGG TATTTCAGCT AGAACCTTCA GACCCAATGT TATGGAGAAA AACTTTAAAG
    AATTTTGAGG TTGAGAGTTT GTTCCAGAAA TACCACAGTA GGCGTAGCTC AAGGATCAAA
    GCCCCGTCTC GTAACACCAT CCAGAAATTT GTTTCACGCA TGTCAAATTC TCATACATTG
    TCGTCATCTA GCACGTCTAC ATCCAGTTCA GAAAACAGTA TAAAAGATGA AGAGGAACAG
    ATGTGTCCTA TTTGCTTGTT GGGCATGCTT GATGAAGAAA GTCTTACAGT GTGTGAAGAC
    GGCTGCAGGA ACAAGCTGCA CCACCACTGC ATGTCCATTT GGGCAGAAGA ATGTAGAAGA
    AATAGAGAAC CTTTAATATG TCCCCTTTGT AGGTCGAAGT GGAGATCCCA CGATTTCTAC
    AGCCACGAGT TGTCCAGCCC TGTGGATTCC CCTTCTCCCC TGCGAGCTCC ACAGCAGCAG
    ACCACCCAGC AGCATGCCTT GGCTGGGTCC CAGCGGAGGA ATCAGGAGGG CAGTCTTACC
    CTTACTCATT ACGGGACTCA GCAGATCCCT CCTTCTTACA AAGATTTAGC TGAGCCTTGG
    ATTCAGGTAT TTGGAATGGA ACTCGTTGGC TGCTTATTTT CTAGAAACTG GAATGTAAGA
    GAGATGGCTC TCAGACGTCT TTCCCACGAC GTTAGTGGGG CTCTGCTGTT GGCAAATGGG
    GAGAGCACTG GAAATTCTGG GAGCAGTAGC GGAAGCGGTC CAGGTGCTGG AGCCACCAGT
    GGGTCTTCCC AGACCAGTAT CTCAGGAGAT GTGGTGGAGG CATGCTGCAA CGTTTTGTCA
    ATGGTCTGTG CTGACCCTGT CTACAAAGTG TACGTTGCTG CTTTAAAAAC GTTGAGAGCC
    ATGCTAGTAT ATACTCCTTG CCATAGTCTG GCAGAAAGAA TCAAACTGCA GAGACTTCTC
    CGGCCAGTTG TAGACACCAT CCTAGTCAAG TGTGCAGACG TCAATAGTCG CACAAGTCAG
    CTGTCTATAT CAACGCTATT GGAATTGTGC AAAGGCCAAG CAGGAGAGTT GGCAGTTGGC
    AGAGAAATAC TTAAAGCTGG ATCCATTGGT ATTGGTGGTG TTGATTATGT CTTAAATTGT
    ATTCTTGGAA ACCAAACTGA ATCAAACAAC TGGCAAGAAC TCCTGGGCCG GCTCTGTCTC
    ATAGATAGAC TGCTGTTGGA ATTTCCTGCT GAATTTTACC CTCATATTGT CAGTACTGAT
    ATTTCACAAG CTGAGCCTGT TGAAGTCAGG TATAAGAAGC TGCTGTCCCT CCTGACCTTT
    GCTTTGCAGT CCATTGATAA TTCCCACTCA ATGGTTGGTA AACTCTCCCG AAGGATATAT
    TTGAGTTCTG CAAGAATGGT TACTGCAGTA CCCCATGTAT TTTCAAAACT GTTAGAAATG
    CTGAACGTTT CTAGTTCCAC TCATTTTACC AGGATGCGGC GTCGTTTGAT GGCTATTGCA
    GATGAGGTGG AAATTGCTGA GGCCATTCAG CTGGGCGTAG AAGACACTTT GGATGGTCGA
    CATGACTGCT TTTTGCAGGC ATCTGCCCCT AACAGCTATC CAGAAGCCAT GGACAATAGT
    TCCTTTGAAC GCATGGACCA TTTAGAGAAA CCTGGAAAAG GACTGTGTGC TACAAAATTG
    AGTGCCAGTT CAGAGGACAT TTCTGACCGA CTGGCCAGCA TTTCAGTAGG ACTTCCTAAT
    TCAACAACAA TGGAGCAACC AAAGCCAATG GTTCAAACAA AGGGCAGACC CCACAGTCAG
    TGTCTGAACG CGTCTCCTGC ATCTCCTCAT TCCCAGTTAA TGTTCCCAGC CTTGTCAACC
    CCTTCTTCAT CAACCCCATC TGTACCATCT GGCACTGGGA CAGATCTCTC TAAGCAGAGA
    CCTCAGGGGT TTGTTCCCTG CAAAATGCCT TCTGCATCTC CTCAAACACA GCGTAAGTTT
    TCTCTACAGT TCCAAAGAAA CTGTTCTGAA AACAAAGACT CAGATAAACT TTCCCCAATC
    TTCACTCAGT CAAGACCCCT ACCCTCTAGT AATATACACA GGCCAAAGCC ATCTCGACCT
    ACCCCAAGTA ATACCAGTAA ACAGGGAGAT GCCTCAAAAA ACAGCATGAC ACTTGATTTG
    AACAGTAGGT CCAAATGTGA TGACAGCTTT GGCAGTAGCA GCAATAGCAG TAATGCTGTT
    ATACCCAGTG ACGAGACAGT GTTCACCCCA GTAGAAGAGA AATGCAGATT AGATGTCAGT
    ACAGAGCTCA ACTCCAGTAT TGAGGACCTT CTTGAAGCGT CTATGCCTTC AAGTGACACA
    ACAGTAACTT TTAAGTCAGA AGTTGCTGTG CTCTCTCCTG AAAAAGATGA AAATGATGAT
    ACCTACAAAG ATGATGTGAA TCATAATCAA AAGTGCAAAG AAAAGATGGA AGCTGAAGAA
    GAAGAAGCTT TAGCGATTGC CATGGCAATG TCAGCATCTC AGGATGCCCT CCCCGTAGTT
    CCTCAACTTC AGGTTGAAAA TGGAGAAGAT ATCATCATTA TTCAACAAGA TACACCAGAG
    ACTCTACCAG GACATACCAA AGCAAAACAG CCTTATAGAG AAGACAGTGA ATGGCTGAAA
    GGTCAGCAGA TAGGCCTTGG CGCCTTCTCT TCTTGTTATC AGGCTCAAGA TGTGGGAACT
    GGAACTTTAA TGGCTGTTAA ACAGGTGACA TATGTCAGAA ATACATCTTC TGAGCAAGAA
    GAAGTAGTAG AAGCATTAAG AGAAGAGATA AGAATGATGA GCCATCTGAA TCATCCAAAC
    ATCATTAGGA TGTTGGGAGC CACATGCGAG AAGAGCAATT ATAATCTCTT CATTGAATGG
    ATGGCAGGGG GATCTGTGGC TCATTTGCTC AGTAAATATG GAGCCTTCAA GGAGTCGGTA
    GTTATTAACT ATACTGAGCA ATTACTTCGT GGCCTTTCAT ATCTCCATGA AAACCAGATC
    ATTCACAGAG ATGTCAAAGG TGCCAATTTG CTCATTGACA GCACAGGTCA GAGACTGAGA
    ATTGCTGACT TTGGAGCTGC AGCCAGGCTG GCATCAAAAG GAACCGGTGC AGGAGAGTTT
    CAGGGACAGT TACTGGGGAC AATTGCATTT ATGGCACCTG AGGTCCTAAG AGGTCAGCAG
    TATGGTAGGA GCTGTGATGT ATGGAGCGTT GGCTGTGCTA TTATAGAAAT GGCTTGTGCT
    AAACCACCTT GGAATGCAGA AAAACACTCC AATCATCTTG CTTTGATATT TAAGATTGCC
    AGTGCAACTA CTGCTCCATC AATTCCTTCA CATTTGTCTC CTGGTTTACG AGATGTGGCT
    CTTCGTTGTT TAGAACTTCA ACCTCAGGAC AGACCTCCAT CGAGAGAGCT ACTGAAGCAT
    CCAGTCTTCC GTACTACGTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.