SHROOM2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SHROOM2
Entrez Gene ID	523644
Full Name	shroom family member 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002700461.4	XP_002700507.2	protein Shroom2
XM_002700461.5	XP_002700507.3	protein Shroom2 isoform X3
XM_024988426.1	XP_024844194.1	protein Shroom2 isoform X2
XM_024988427.1	XP_024844195.1	protein Shroom2 isoform X4
XM_024988428.1	XP_024844196.1	protein Shroom2 isoform X5
XM_024988429.1	XP_024844197.1	protein Shroom2 isoform X5
XM_024988425.1	XP_024844193.1	protein Shroom2 isoform X1
XM_601946.6	XP_601946.4	protein Shroom2

Pathways from BioSystems
Gene Ontology

The following SHROOM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHROOM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238593	XM_024988429.1 Latest version!	Bos taurus shroom family member 2 (SHROOM2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa238593	XM_024988428.1 Latest version!	Bos taurus shroom family member 2 (SHROOM2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa238592	XM_024988427.1 Latest version!	Bos taurus shroom family member 2 (SHROOM2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa238589	XM_024988425.1 Latest version!	Bos taurus shroom family member 2 (SHROOM2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa238590	XM_024988426.1 Latest version!	Bos taurus shroom family member 2 (SHROOM2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa238591	XM_002700461.5 Latest version!	Bos taurus shroom family member 2 (SHROOM2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa15217	XM_002700461.4 Latest version!	Bos taurus shroom family member 2 (SHROOM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa15217	XM_601946.6 Latest version!	Bos taurus shroom family member 2 (SHROOM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHROOM2 ( XM_024988428.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844196.1 Bos taurus shroom family member 2 (SHROOM2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa238593
Clone ID Related Accession (Same CDS sequence)	XM_024988428.1 , XM_024988429.1
Accession Version	XM_024988428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein Shroom2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCT CGCGCTCGCC GTCGCCGCAG TTCGCGCCGC AGAAGCTGAC GGACAAGCCC 
CCTCTGCTCA TCCAGGACGA GAATGCTGCC AGAATCGAGC GGGTGATGGA CAACAACACC 
ACGGTGAAGA TGGTGCCCAT CAAGATAGTG CATTCGGAGA GCCAGCCGGA GAAGGAGAGC 
CGCCAGGCAC TGGCCCGCGT CCCCGAGCCA CCTCCGCTGC CCCGGGGCCT CGAGCGGGAC 
CAGATCAAGA CGCTCAGCAC GTCTGAGCAG TCCTACTCAC GCTTCTGCCC CTACAGCCGC 
CAGGGTGCCG AGCCCCAGCC CGCCACCGGC GCCCTGGGGC CCCCCGCCAA GGAAGGCCGG 
GCCTCCCCTC CTGTGCTCAG CTACGTGAAG GCCAAAGACA GGACCGCCGA CGACCTCAAG 
TCAGAGGAGC TGGCCCGCGA GATCGCGGGC AGGGATAAGT CGCTGGCGGA CATCCTAGAC 
CCGGGCGTGA GGATGAGAAC CACCATGGAC CTCATGGAGG GCATCTTCCC CAAAGACGAG 
CACCTCCTGG AAGGCGCACA GCAACGGAGG AAGCTGCTGC CCAAGGGGCC CTCGCCCAGG 
ACGGCGGAGG AGAGGAGGGA GGAGACCAGT GTGCTGGCAG CCGCACCCTT GGCCACCAAC 
TCCGCCTACT ACAGCACGTC AGCTCCCAAG GCGGAGCTGC TGATAAAGAT GAAGGACCTG 
CAGGAGCAGC AGGAGCCAGA GGAGGACTCA GGCAGTGACT TGGACCACGA CCTGTCAGTC 
AAAAAGCAGG AGCTCATTGA GAGCCTTGGC CGCAAGCTGC AGGTGCTGCG GGAGGCGCGG 
GAGAGCCTGC TGGAGGACGT GCAGGCCAAC AGCGCGCTGG GCGACGAGGT GGAGGCCCTG 
GTCAAGGCCG TGTGCAAGCC CAATGAGTTT GACAAGTTCC GCATGTTCAT CGGGGACCTC 
GACAAGGTGG TGAACCTCCT GCTGTCGCTG TCCGGCCGCC TGGCCCGCGT GGAGAACGCC 
CTCAACAACC TGGACGACAG CACGCCTCCG GGCGATCGGC AATCCCTGCT CGAGAAGCAG 
CGCGTCCTGA TCCAGCAGCA CGAAGACGCC AGGGAGCTGA AGGAGAACCT GGACCGCCGG 
GAAGGCATCG TCTTCGACAT CCTGGTCAGC TACCTGGGCC CCGAGAGCCT GGCGGACTAC 
GAGCACTTCG TCAAGATGAA GTCGGCGCTC ATCATCGAAC AGCGCGAGCT GGAAGACAAA 
ATACACCTGG GTGAGGAGCA GCTGAAGTGC CTCTTGGACA GCCTGCAGCC CGAGAGAGGC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844196.1
CDS	585..1910
Translation	MDSSRSPSPQFAPQKLTDKPPLLIQDENAARIERVMDNNTTVKMVPIKIVHSESQPEKESRQALARVPEPPPLPRGLERDQIKTLSTSEQSYSRFCPYSRQGAEPQPATGALGPPAKEGRASPPVLSYVKAKDRTADDLKSEELAREIAGRDKSLADILDPGVRMRTTMDLMEGIFPKDEHLLEGAQQRRKLLPKGPSPRTAEERREETSVLAAAPLATNSAYYSTSAPKAELLIKMKDLQEQQEPEEDSGSDLDHDLSVKKQELIESLGRKLQVLREARESLLEDVQANSALGDEVEALVKAVCKPNEFDKFRMFIGDLDKVVNLLLSLSGRLARVENALNNLDDSTPPGDRQSLLEKQRVLIQQHEDARELKENLDRREGIVFDILVSYLGPESLADYEHFVKMKSALIIEQRELEDKIHLGEEQLKCLLDSLQPERGK

Target ORF information:

RefSeq Version	XM_024988428.1
Organism	Bos taurus(cattle)
Definition	Bos taurus shroom family member 2 (SHROOM2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988428.1

ORF Insert Sequence:

ATGGACAGCT CGCGCTCGCC GTCGCCGCAG TTCGCGCCGC AGAAGCTGAC GGACAAGCCC 
CCTCTGCTCA TCCAGGACGA GAATGCTGCC AGAATCGAGC GGGTGATGGA CAACAACACC 
ACGGTGAAGA TGGTGCCCAT CAAGATAGTG CATTCGGAGA GCCAGCCGGA GAAGGAGAGC 
CGCCAGGCAC TGGCCCGCGT CCCCGAGCCA CCTCCGCTGC CCCGGGGCCT CGAGCGGGAC 
CAGATCAAGA CGCTCAGCAC GTCTGAGCAG TCCTACTCAC GCTTCTGCCC CTACAGCCGC 
CAGGGTGCCG AGCCCCAGCC CGCCACCGGC GCCCTGGGGC CCCCCGCCAA GGAAGGCCGG 
GCCTCCCCTC CTGTGCTCAG CTACGTGAAG GCCAAAGACA GGACCGCCGA CGACCTCAAG 
TCAGAGGAGC TGGCCCGCGA GATCGCGGGC AGGGATAAGT CGCTGGCGGA CATCCTAGAC 
CCGGGCGTGA GGATGAGAAC CACCATGGAC CTCATGGAGG GCATCTTCCC CAAAGACGAG 
CACCTCCTGG AAGGCGCACA GCAACGGAGG AAGCTGCTGC CCAAGGGGCC CTCGCCCAGG 
ACGGCGGAGG AGAGGAGGGA GGAGACCAGT GTGCTGGCAG CCGCACCCTT GGCCACCAAC 
TCCGCCTACT ACAGCACGTC AGCTCCCAAG GCGGAGCTGC TGATAAAGAT GAAGGACCTG 
CAGGAGCAGC AGGAGCCAGA GGAGGACTCA GGCAGTGACT TGGACCACGA CCTGTCAGTC 
AAAAAGCAGG AGCTCATTGA GAGCCTTGGC CGCAAGCTGC AGGTGCTGCG GGAGGCGCGG 
GAGAGCCTGC TGGAGGACGT GCAGGCCAAC AGCGCGCTGG GCGACGAGGT GGAGGCCCTG 
GTCAAGGCCG TGTGCAAGCC CAATGAGTTT GACAAGTTCC GCATGTTCAT CGGGGACCTC 
GACAAGGTGG TGAACCTCCT GCTGTCGCTG TCCGGCCGCC TGGCCCGCGT GGAGAACGCC 
CTCAACAACC TGGACGACAG CACGCCTCCG GGCGATCGGC AATCCCTGCT CGAGAAGCAG 
CGCGTCCTGA TCCAGCAGCA CGAAGACGCC AGGGAGCTGA AGGAGAACCT GGACCGCCGG 
GAAGGCATCG TCTTCGACAT CCTGGTCAGC TACCTGGGCC CCGAGAGCCT GGCGGACTAC 
GAGCACTTCG TCAAGATGAA GTCGGCGCTC ATCATCGAAC AGCGCGAGCT GGAAGACAAA 
ATACACCTGG GTGAGGAGCA GCTGAAGTGC CTCTTGGACA GCCTGCAGCC CGAGAGAGGC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHROOM2 ( XM_024988429.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844197.1 Bos taurus shroom family member 2 (SHROOM2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa238593
Clone ID Related Accession (Same CDS sequence)	XM_024988428.1 , XM_024988429.1
Accession Version	XM_024988429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein Shroom2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCT CGCGCTCGCC GTCGCCGCAG TTCGCGCCGC AGAAGCTGAC GGACAAGCCC 
CCTCTGCTCA TCCAGGACGA GAATGCTGCC AGAATCGAGC GGGTGATGGA CAACAACACC 
ACGGTGAAGA TGGTGCCCAT CAAGATAGTG CATTCGGAGA GCCAGCCGGA GAAGGAGAGC 
CGCCAGGCAC TGGCCCGCGT CCCCGAGCCA CCTCCGCTGC CCCGGGGCCT CGAGCGGGAC 
CAGATCAAGA CGCTCAGCAC GTCTGAGCAG TCCTACTCAC GCTTCTGCCC CTACAGCCGC 
CAGGGTGCCG AGCCCCAGCC CGCCACCGGC GCCCTGGGGC CCCCCGCCAA GGAAGGCCGG 
GCCTCCCCTC CTGTGCTCAG CTACGTGAAG GCCAAAGACA GGACCGCCGA CGACCTCAAG 
TCAGAGGAGC TGGCCCGCGA GATCGCGGGC AGGGATAAGT CGCTGGCGGA CATCCTAGAC 
CCGGGCGTGA GGATGAGAAC CACCATGGAC CTCATGGAGG GCATCTTCCC CAAAGACGAG 
CACCTCCTGG AAGGCGCACA GCAACGGAGG AAGCTGCTGC CCAAGGGGCC CTCGCCCAGG 
ACGGCGGAGG AGAGGAGGGA GGAGACCAGT GTGCTGGCAG CCGCACCCTT GGCCACCAAC 
TCCGCCTACT ACAGCACGTC AGCTCCCAAG GCGGAGCTGC TGATAAAGAT GAAGGACCTG 
CAGGAGCAGC AGGAGCCAGA GGAGGACTCA GGCAGTGACT TGGACCACGA CCTGTCAGTC 
AAAAAGCAGG AGCTCATTGA GAGCCTTGGC CGCAAGCTGC AGGTGCTGCG GGAGGCGCGG 
GAGAGCCTGC TGGAGGACGT GCAGGCCAAC AGCGCGCTGG GCGACGAGGT GGAGGCCCTG 
GTCAAGGCCG TGTGCAAGCC CAATGAGTTT GACAAGTTCC GCATGTTCAT CGGGGACCTC 
GACAAGGTGG TGAACCTCCT GCTGTCGCTG TCCGGCCGCC TGGCCCGCGT GGAGAACGCC 
CTCAACAACC TGGACGACAG CACGCCTCCG GGCGATCGGC AATCCCTGCT CGAGAAGCAG 
CGCGTCCTGA TCCAGCAGCA CGAAGACGCC AGGGAGCTGA AGGAGAACCT GGACCGCCGG 
GAAGGCATCG TCTTCGACAT CCTGGTCAGC TACCTGGGCC CCGAGAGCCT GGCGGACTAC 
GAGCACTTCG TCAAGATGAA GTCGGCGCTC ATCATCGAAC AGCGCGAGCT GGAAGACAAA 
ATACACCTGG GTGAGGAGCA GCTGAAGTGC CTCTTGGACA GCCTGCAGCC CGAGAGAGGC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844197.1
CDS	615..1940
Translation	MDSSRSPSPQFAPQKLTDKPPLLIQDENAARIERVMDNNTTVKMVPIKIVHSESQPEKESRQALARVPEPPPLPRGLERDQIKTLSTSEQSYSRFCPYSRQGAEPQPATGALGPPAKEGRASPPVLSYVKAKDRTADDLKSEELAREIAGRDKSLADILDPGVRMRTTMDLMEGIFPKDEHLLEGAQQRRKLLPKGPSPRTAEERREETSVLAAAPLATNSAYYSTSAPKAELLIKMKDLQEQQEPEEDSGSDLDHDLSVKKQELIESLGRKLQVLREARESLLEDVQANSALGDEVEALVKAVCKPNEFDKFRMFIGDLDKVVNLLLSLSGRLARVENALNNLDDSTPPGDRQSLLEKQRVLIQQHEDARELKENLDRREGIVFDILVSYLGPESLADYEHFVKMKSALIIEQRELEDKIHLGEEQLKCLLDSLQPERGK

Target ORF information:

RefSeq Version	XM_024988429.1
Organism	Bos taurus(cattle)
Definition	Bos taurus shroom family member 2 (SHROOM2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988429.1

ORF Insert Sequence:

ATGGACAGCT CGCGCTCGCC GTCGCCGCAG TTCGCGCCGC AGAAGCTGAC GGACAAGCCC 
CCTCTGCTCA TCCAGGACGA GAATGCTGCC AGAATCGAGC GGGTGATGGA CAACAACACC 
ACGGTGAAGA TGGTGCCCAT CAAGATAGTG CATTCGGAGA GCCAGCCGGA GAAGGAGAGC 
CGCCAGGCAC TGGCCCGCGT CCCCGAGCCA CCTCCGCTGC CCCGGGGCCT CGAGCGGGAC 
CAGATCAAGA CGCTCAGCAC GTCTGAGCAG TCCTACTCAC GCTTCTGCCC CTACAGCCGC 
CAGGGTGCCG AGCCCCAGCC CGCCACCGGC GCCCTGGGGC CCCCCGCCAA GGAAGGCCGG 
GCCTCCCCTC CTGTGCTCAG CTACGTGAAG GCCAAAGACA GGACCGCCGA CGACCTCAAG 
TCAGAGGAGC TGGCCCGCGA GATCGCGGGC AGGGATAAGT CGCTGGCGGA CATCCTAGAC 
CCGGGCGTGA GGATGAGAAC CACCATGGAC CTCATGGAGG GCATCTTCCC CAAAGACGAG 
CACCTCCTGG AAGGCGCACA GCAACGGAGG AAGCTGCTGC CCAAGGGGCC CTCGCCCAGG 
ACGGCGGAGG AGAGGAGGGA GGAGACCAGT GTGCTGGCAG CCGCACCCTT GGCCACCAAC 
TCCGCCTACT ACAGCACGTC AGCTCCCAAG GCGGAGCTGC TGATAAAGAT GAAGGACCTG 
CAGGAGCAGC AGGAGCCAGA GGAGGACTCA GGCAGTGACT TGGACCACGA CCTGTCAGTC 
AAAAAGCAGG AGCTCATTGA GAGCCTTGGC CGCAAGCTGC AGGTGCTGCG GGAGGCGCGG 
GAGAGCCTGC TGGAGGACGT GCAGGCCAAC AGCGCGCTGG GCGACGAGGT GGAGGCCCTG 
GTCAAGGCCG TGTGCAAGCC CAATGAGTTT GACAAGTTCC GCATGTTCAT CGGGGACCTC 
GACAAGGTGG TGAACCTCCT GCTGTCGCTG TCCGGCCGCC TGGCCCGCGT GGAGAACGCC 
CTCAACAACC TGGACGACAG CACGCCTCCG GGCGATCGGC AATCCCTGCT CGAGAAGCAG 
CGCGTCCTGA TCCAGCAGCA CGAAGACGCC AGGGAGCTGA AGGAGAACCT GGACCGCCGG 
GAAGGCATCG TCTTCGACAT CCTGGTCAGC TACCTGGGCC CCGAGAGCCT GGCGGACTAC 
GAGCACTTCG TCAAGATGAA GTCGGCGCTC ATCATCGAAC AGCGCGAGCT GGAAGACAAA 
ATACACCTGG GTGAGGAGCA GCTGAAGTGC CTCTTGGACA GCCTGCAGCC CGAGAGAGGC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHROOM2 ( XM_024988427.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844195.1 Bos taurus shroom family member 2 (SHROOM2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa238592
Clone ID Related Accession (Same CDS sequence)	XM_024988427.1
Accession Version	XM_024988427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein Shroom2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCTC GATGGGAAGC AGGGGACCCG GAGGAGCACA CGGAGCTTTC TTCGGCGGCA 
CCGGCCGAGG ACAGAAGCCC CACCCCAAGA CAAGCAGATG GTCAGCGTTC GGAGGACCAC 
CCAGGAGCAC TGCGGGACCC CCGGCAAACC TCTGAACCCC CTGGCAGCAG GGAAGGGCCC 
GAGGTGTCCC TGGAGGCCCG CGGCCGGGCG GGCACTCTCC CTCGCGATTA CCGGTACCTG 
GAGCCGCGCA CGCCCCTGGA GCCACCCGCC CAGGGGCAGG AGCCCCGGCC CCTGAGCGCC 
GCCGCGACAG CCCGGAGACC CGCCCTACAG AGGCCACAGC CGCCCAAGCG CGAGCTCTGG 
CCGCCGCCAG GGGACACGCC CAGCCGGCCT CACACCTCCG TGGCCTCCTC GGAGGCGGTG 
GACACGCGGA GCACGCGGCG CGTGGAGGAG CCCACGGCCC GGCCCTGGCG GGAGGCGCTG 
CTTCCCGCCC CGTTCCGGCC CCTGCAGACC TCCGCCATGG ACAGCTCGCG CTCGCCGTCG 
CCGCAGTTCG CGCCGCAGAA GCTGACGGAC AAGCCCCCTC TGCTCATCCA GGACGAGAAT 
GCTGCCAGAA TCGAGCGGGT GATGGACAAC AACACCACGG TGAAGATGGT GCCCATCAAG 
ATAGTGCATT CGGAGAGCCA GCCGGAGAAG GAGAGCCGCC AGGCACTGGC CCGCGTCCCC 
GAGCCACCTC CGCTGCCCCG GGGCCTCGAG CGGGACCAGA TCAAGACGCT CAGCACGTCT 
GAGCAGTCCT ACTCACGCTT CTGCCCCTAC AGCCGCCAGG GTGCCGAGCC CCAGCCCGCC 
ACCGGCGCCC TGGGGCCCCC CGCCAAGGAA GGCCGGGCCT CCCCTCCTGT GCTCAGCTAC 
GTGAAGGCCA AAGACAGGAC CGCCGACGAC CTCAAGTCAG AGGAGCTGGC CCGCGAGATC 
GCGGGCAGGG ATAAGTCGCT GGCGGACATC CTAGACCCGG GCGTGAGGAT GAGAACCACC 
ATGGACCTCA TGGAGGGCAT CTTCCCCAAA GACGAGCACC TCCTGGAAGG CGCACAGCAA 
CGGAGGAAGC TGCTGCCCAA GGGGCCCTCG CCCAGGACGG CGGAGGAGAG GAGGGAGGAG 
ACCAGTGTGC TGGCAGCCGC ACCCTTGGCC ACCAACTCCG CCTACTACAG CACGTCAGCT 
CCCAAGGCGG AGCTGCTGAT AAAGATGAAG GACCTGCAGG AGCAGCAGGA GCCAGAGGAG 
GACTCAGGCA GTGACTTGGA CCACGACCTG TCAGTCAAAA AGCAGGAGCT CATTGAGAGC 
CTTGGCCGCA AGCTGCAGGT GCTGCGGGAG GCGCGGGAGA GCCTGCTGGA GGACGTGCAG 
GCCAACAGCG CGCTGGGCGA CGAGGTGGAG GCCCTGGTCA AGGCCGTGTG CAAGCCCAAT 
GAGTTTGACA AGTTCCGCAT GTTCATCGGG GACCTCGACA AGGTGGTGAA CCTCCTGCTG 
TCGCTGTCCG GCCGCCTGGC CCGCGTGGAG AACGCCCTCA ACAACCTGGA CGACAGCACG 
CCTCCGGGCG ATCGGCAATC CCTGCTCGAG AAGCAGCGCG TCCTGATCCA GCAGCACGAA 
GACGCCAGGG AGCTGAAGGA GAACCTGGAC CGCCGGGAAG GCATCGTCTT CGACATCCTG 
GTCAGCTACC TGGGCCCCGA GAGCCTGGCG GACTACGAGC ACTTCGTCAA GATGAAGTCG 
GCGCTCATCA TCGAACAGCG CGAGCTGGAA GACAAAATAC ACCTGGGTGA GGAGCAGCTG 
AAGTGCCTCT TGGACAGCCT GCAGCCCGAG AGAGGCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844195.1
CDS	131..1972
Translation	MEPRWEAGDPEEHTELSSAAPAEDRSPTPRQADGQRSEDHPGALRDPRQTSEPPGSREGPEVSLEARGRAGTLPRDYRYLEPRTPLEPPAQGQEPRPLSAAATARRPALQRPQPPKRELWPPPGDTPSRPHTSVASSEAVDTRSTRRVEEPTARPWREALLPAPFRPLQTSAMDSSRSPSPQFAPQKLTDKPPLLIQDENAARIERVMDNNTTVKMVPIKIVHSESQPEKESRQALARVPEPPPLPRGLERDQIKTLSTSEQSYSRFCPYSRQGAEPQPATGALGPPAKEGRASPPVLSYVKAKDRTADDLKSEELAREIAGRDKSLADILDPGVRMRTTMDLMEGIFPKDEHLLEGAQQRRKLLPKGPSPRTAEERREETSVLAAAPLATNSAYYSTSAPKAELLIKMKDLQEQQEPEEDSGSDLDHDLSVKKQELIESLGRKLQVLREARESLLEDVQANSALGDEVEALVKAVCKPNEFDKFRMFIGDLDKVVNLLLSLSGRLARVENALNNLDDSTPPGDRQSLLEKQRVLIQQHEDARELKENLDRREGIVFDILVSYLGPESLADYEHFVKMKSALIIEQRELEDKIHLGEEQLKCLLDSLQPERGK

Target ORF information:

RefSeq Version	XM_024988427.1
Organism	Bos taurus(cattle)
Definition	Bos taurus shroom family member 2 (SHROOM2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988427.1

ORF Insert Sequence:

ATGGAACCTC GATGGGAAGC AGGGGACCCG GAGGAGCACA CGGAGCTTTC TTCGGCGGCA 
CCGGCCGAGG ACAGAAGCCC CACCCCAAGA CAAGCAGATG GTCAGCGTTC GGAGGACCAC 
CCAGGAGCAC TGCGGGACCC CCGGCAAACC TCTGAACCCC CTGGCAGCAG GGAAGGGCCC 
GAGGTGTCCC TGGAGGCCCG CGGCCGGGCG GGCACTCTCC CTCGCGATTA CCGGTACCTG 
GAGCCGCGCA CGCCCCTGGA GCCACCCGCC CAGGGGCAGG AGCCCCGGCC CCTGAGCGCC 
GCCGCGACAG CCCGGAGACC CGCCCTACAG AGGCCACAGC CGCCCAAGCG CGAGCTCTGG 
CCGCCGCCAG GGGACACGCC CAGCCGGCCT CACACCTCCG TGGCCTCCTC GGAGGCGGTG 
GACACGCGGA GCACGCGGCG CGTGGAGGAG CCCACGGCCC GGCCCTGGCG GGAGGCGCTG 
CTTCCCGCCC CGTTCCGGCC CCTGCAGACC TCCGCCATGG ACAGCTCGCG CTCGCCGTCG 
CCGCAGTTCG CGCCGCAGAA GCTGACGGAC AAGCCCCCTC TGCTCATCCA GGACGAGAAT 
GCTGCCAGAA TCGAGCGGGT GATGGACAAC AACACCACGG TGAAGATGGT GCCCATCAAG 
ATAGTGCATT CGGAGAGCCA GCCGGAGAAG GAGAGCCGCC AGGCACTGGC CCGCGTCCCC 
GAGCCACCTC CGCTGCCCCG GGGCCTCGAG CGGGACCAGA TCAAGACGCT CAGCACGTCT 
GAGCAGTCCT ACTCACGCTT CTGCCCCTAC AGCCGCCAGG GTGCCGAGCC CCAGCCCGCC 
ACCGGCGCCC TGGGGCCCCC CGCCAAGGAA GGCCGGGCCT CCCCTCCTGT GCTCAGCTAC 
GTGAAGGCCA AAGACAGGAC CGCCGACGAC CTCAAGTCAG AGGAGCTGGC CCGCGAGATC 
GCGGGCAGGG ATAAGTCGCT GGCGGACATC CTAGACCCGG GCGTGAGGAT GAGAACCACC 
ATGGACCTCA TGGAGGGCAT CTTCCCCAAA GACGAGCACC TCCTGGAAGG CGCACAGCAA 
CGGAGGAAGC TGCTGCCCAA GGGGCCCTCG CCCAGGACGG CGGAGGAGAG GAGGGAGGAG 
ACCAGTGTGC TGGCAGCCGC ACCCTTGGCC ACCAACTCCG CCTACTACAG CACGTCAGCT 
CCCAAGGCGG AGCTGCTGAT AAAGATGAAG GACCTGCAGG AGCAGCAGGA GCCAGAGGAG 
GACTCAGGCA GTGACTTGGA CCACGACCTG TCAGTCAAAA AGCAGGAGCT CATTGAGAGC 
CTTGGCCGCA AGCTGCAGGT GCTGCGGGAG GCGCGGGAGA GCCTGCTGGA GGACGTGCAG 
GCCAACAGCG CGCTGGGCGA CGAGGTGGAG GCCCTGGTCA AGGCCGTGTG CAAGCCCAAT 
GAGTTTGACA AGTTCCGCAT GTTCATCGGG GACCTCGACA AGGTGGTGAA CCTCCTGCTG 
TCGCTGTCCG GCCGCCTGGC CCGCGTGGAG AACGCCCTCA ACAACCTGGA CGACAGCACG 
CCTCCGGGCG ATCGGCAATC CCTGCTCGAG AAGCAGCGCG TCCTGATCCA GCAGCACGAA 
GACGCCAGGG AGCTGAAGGA GAACCTGGAC CGCCGGGAAG GCATCGTCTT CGACATCCTG 
GTCAGCTACC TGGGCCCCGA GAGCCTGGCG GACTACGAGC ACTTCGTCAA GATGAAGTCG 
GCGCTCATCA TCGAACAGCG CGAGCTGGAA GACAAAATAC ACCTGGGTGA GGAGCAGCTG 
AAGTGCCTCT TGGACAGCCT GCAGCCCGAG AGAGGCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHROOM2 ( XM_024988425.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844193.1 Bos taurus shroom family member 2 (SHROOM2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa238589
Clone ID Related Accession (Same CDS sequence)	XM_024988425.1
Accession Version	XM_024988425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein Shroom2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCACC TTGAGCCAGG TGACCGTTCG GTAGGCTGGC CTCAGAGAAA ACTCTGCAGG 
CTGCCTGCCA AGTCAGATCT TTGTCGTTCC TCCCTGGCTC CTGCAGTCTT TCTCTCTGGA 
GTCTCTGTGT GCAGACAGCG CTGCTGTCTC CGGCGTGGGA AACAGAAAAG TACCAAGGTT 
GAAGAAGGCA GCAAAGCCGC GGCCGTGGAC AAACTGCTGG CCGGAGACGA GATCGTGGGC 
ATCAATGATG TCGGTCTGTC CGGGTTTAGA CAGGAAGCCA TCTGCCTGGT GAAGGGGTCA 
CATAAGACCC TGAAGCTGGT GGTGAAAAGG AGGAATGAAC TGAACTGGAG GCCACACTCC 
TGGCATGCCA CCAAGTTCTC TGACAGCCAG TCTGAGCCAG CTGCACCTCT GCTTCCCTGC 
CCCTCTCGGC CGAGCCGGTA CCACGCCAGC TCCTCCTCCC AGGACCTGTC GGGCGGTGCC 
TGGGAGCCAG CGAACCTGCA GCGCACCTCG GACCACTTCA GCTCGCTGGG GAGCGTCGAC 
AGCCTGGACC CATCCTCCCA GCCCGACCCG TCGGGGCGCC TCTCCGCGGC CAAGTCCAGC 
AGCAGCATCG ACCGCCTGGG CGGTGCCAGC AAGCGGGACT CAGCTTATGG TTCATTCTCC 
ACGCGCTCCA GCACCCCGGA CCACACCCTG CCCAAGGCAG ACGCGTCCTC GGCCGAGAAT 
ATCCTCTACA AAGTGGGCCT GTGGGAGGCC TCCGCTGCAG CGGACAGACG GCCGGGCCTG 
GCGGCTGGCG ACCCTCAGGG CACCGAGGAG CCCCTGGGGT GCTTCCCACC CCGGGTCCCT 
GAGGACGGCC CCGAGCCCAA GCTAGCTGCT TGCGGGCGGT CCAGCTCCGG GCCCGTCTGG 
TATGTTCCGG ATAAGAGAAG AGCCCCTCCG TCCCCTCCCC CGCCGCCTCC CCCGCTCCGC 
CGTGACAGCT TCGCTGCCAC CAGGATCCAC GAGCAGACCC AGGGCCCCAC GGTCTCAGCA 
GACGCAGCCC CAGCGCAGCC CTTGCCGGGC CTGAGCTGCG TGCAGCCCCA CGGTGGCGAC 
TGGAAGAGAC CTGAACCCGC TGATCAGCTG TTGAGGCCGG CGCGCGGGGC TGACGGCCGG 
AGAGCTGCGA GCTCGGGGTG CGCTCCAGGG GCACATGTGG ACTGTGGGTG GCCGGCCTGC 
GACAGCGGGT CCCGGGCCCT CTTGGGGGCC CCCAGCCGGC TGCAGGCTTC TCTCTCGAGC 
ACTGACGTGC GCCGCCCGCA GCCCTCGCCC GTGTGCCAAC ACCCGCGCCA CTTGAGCGAC 
GAGGGCCCCT TCTTCCGCAA GGGCCCCAGG GCCACCACGT GGCTAGGGGA GCCCCTCCCC 
GCTGACTGCC TCGGGGACAA CACCCCTGCT GCCATTGTCA TCGGAGGGCC ACGCGCTGCA 
GACCAGAGGG GCGACTGCAG CGCGCAGAGC CGCTACTACT GCGTGATGTC CAGACCCGGT 
CCCCAGTGGG GCGCCCCGGC CCCTCAGCCC CGCTGCTACC CGTCCCGGCT GGAGGGCGCC 
CCGGGCGCAC GGCAGAGAAG CAGGGCAGAC CCGGTGGACA GGGCACCCGA GGACCCACCG 
GGGGCTGGCC TCCTGGACAG AGGTGGCAGG AAGAGGGTGG TGGGCAGCTT GGACGACGGG 
GGCGGCGAGA TCTGCCCCCT GCAGACGCCC ATGCTGCACT CGCTGAGCCG GGAAAGGGCG 
TGCCCGCCCG AGACCCGCCA CAAGGACATG GCGCCGGCGC CGCCTGAGGC CCTGGCAGGC 
AAGCCGCTCC GGAGGAGTGA CCGCTTCGCC ACCACCCTGA GGAATGAGAT CCAGCAGCGG 
CGAGCCAGGC TGCAGAAGAG CCGGAGCACG GCCACCTTGG CCAGCTCCGC GGAGGAGGAG 
GGGGAGGTGG AAGACGACGT GGAGACTGAG GCGCGCGGCT CCTGGGCGCA GCCAGTGGGC 
GCCCCGGAGA CCACCTTCCC CAGCGGCACC TATAAGGACC ACTTGAAGGA GGCGCAGGCC 
CGCGTCCTGA GGGCCACGTC GTTCCGGCGC CGTGACCTGG ATCTCAGCCT GGCGGACCCG 
GAGCCAGCCC CCCGCGTCTG GGAGGGGGCC CCGGGTGCCA GGCCGCCCTT GTGCGCTGCC 
GGCAGTGGCA GTGGCAGCGG CAGCGCGCGC ATCGGGAGCC GGAGGCGCTT CACTGCCGAG 
CAGAAACTGA AGTCCTACTC GGAGCCCGAG AAGATGAACG CGGTGGGGCT GACGGGGGAC 
CAGAGCCCTG CACCCCATGA TGAGACGCTG TGGACCTTTG CTGACAGGTG GAAGTTTTTT 
GAGGAAACCA GCAAGCCCGT GGGCCCCCAG CCCGGGCCGA GGCCGGCGCC CTGTGGCTTC 
CCCAGGGAGA AGCCGGAGAT GCTGTGGACA GTGGGCCCTG GAAAAGACGG AAAGGCGGGG 
AGACCCATCC CGCCAGAGCG GCTCGGCACC TTCGCAGAGT ATCAAGCCTC CTGGAGGGAA 
CAGAGGAAAG TCCCGGAAGC CAGGGGTGGC GGGAGGTACC ACTCCACTGA CAACATCCTC 
GACGTGGGTG TGGACCAGGA AGAGAGGCCA CAGTACGTCC ACGAAAGGTC CCGGTCATCA 
CCTTCCACGG ACCTCTACAA GCAGGACGCC TCCATGGAAC CTCGATGGGA AGCAGGGGAC 
CCGGAGGAGC ACACGGAGCT TTCTTCGGCG GCACCGGCCG AGGACAGAAG CCCCACCCCA 
AGACAAGCAG ATGGTCAGCG TTCGGAGGAC CACCCAGGAG CACTGCGGGA CCCCCGGCAA 
ACCTCTGAAC CCCCTGGCAG CAGGGAAGGG CCCGAGGTGT CCCTGGAGGC CCGCGGCCGG 
GCGGGCACTC TCCCTCGCGA TTACCGGTAC CTGGAGCCGC GCACGCCCCT GGAGCCACCC 
GCCCAGGGGC AGGAGCCCCG GCCCCTGAGC GCCGCCGCGA CAGCCCGGAG ACCCGCCCTA 
CAGAGGCCAC AGCCGCCCAA GCGCGAGCTC TGGCCGCCGC CAGGGGACAC GCCCAGCCGG 
CCTCACACCT CCGTGGCCTC CTCGGAGGCG GTGGACACGC GGAGCACGCG GCGCGTGGAG 
GAGCCCACGG CCCGGCCCTG GCGGGAGGCG CTGCTTCCCG CCCCGTTCCG GCCCCTGCAG 
ACCTCCGCCA TGGACAGCTC GCGCTCGCCG TCGCCGCAGT TCGCGCCGCA GAAGCTGACG 
GACAAGCCCC CTCTGCTCAT CCAGGACGAG AATGCTGCCA GAATCGAGCG GGTGATGGAC 
AACAACACCA CGGTGAAGAT GGTGCCCATC AAGATAGTGC ATTCGGAGAG CCAGCCGGAG 
AAGGAGAGCC GCCAGGCACT GGCCCGCGTC CCCGAGCCAC CTCCGCTGCC CCGGGGCCTC 
GAGCGGGACC AGATCAAGAC GCTCAGCACG TCTGAGCAGT CCTACTCACG CTTCTGCCCC 
TACAGCCGCC AGGGTGCCGA GCCCCAGCCC GCCACCGGCG CCCTGGGGCC CCCCGCCAAG 
GAAGGCCGGG CCTCCCCTCC TGTGCTCAGC TACGTGAAGG CCAAAGACAG GACCGCCGAC 
GACCTCAAGT CAGAGGAGCT GGCCCGCGAG ATCGCGGGCA GGGATAAGTC GCTGGCGGAC 
ATCCTAGACC CGGGCGTGAG GATGAGAACC ACCATGGACC TCATGGAGGG CATCTTCCCC 
AAAGACGAGC ACCTCCTGGA AGGCGCACAG CAACGGAGGA AGCTGCTGCC CAAGGGGCCC 
TCGCCCAGGA CGGCGGAGGA GAGGAGGGAG GAGACCAGTG TGCTGGCAGC CGCACCCTTG 
GCCACCAACT CCGCCTACTA CAGCACGTCA GCTCCCAAGG CGGAGCTGCT GATAAAGATG 
AAGGACCTGC AGGAGCAGCA GGAGCCAGAG GAGGACTCAG GCAGTGACTT GGACCACGAC 
CTGTCAGTCA AAAAGCAGGA GCTCATTGAG AGCCTTGGCC GCAAGCTGCA GGTGCTGCGG 
GAGGCGCGGG AGAGCCTGCT GGAGGACGTG CAGGCCAACA GCGCGCTGGG CGACGAGGTG 
GAGGCCCTGG TCAAGGCCGT GTGCAAGCCC AATGAGTTTG ACAAGTTCCG CATGTTCATC 
GGGGACCTCG ACAAGGTGGT GAACCTCCTG CTGTCGCTGT CCGGCCGCCT GGCCCGCGTG 
GAGAACGCCC TCAACAACCT GGACGACAGC ACGCCTCCGG GCGATCGGCA ATCCCTGCTC 
GAGAAGCAGC GCGTCCTGAT CCAGCAGCAC GAAGACGCCA GGGAGCTGAA GGAGAACCTG 
GACCGCCGGG AAGGCATCGT CTTCGACATC CTGGTCAGCT ACCTGGGCCC CGAGAGCCTG 
GCGGACTACG AGCACTTCGT CAAGATGAAG TCGGCGCTCA TCATCGAACA GCGCGAGCTG 
GAAGACAAAA TACACCTGGG TGAGGAGCAG CTGAAGTGCC TCTTGGACAG CCTGCAGCCC 
GAGAGAGGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844193.1
CDS	322..4836
Translation	MPHLEPGDRSVGWPQRKLCRLPAKSDLCRSSLAPAVFLSGVSVCRQRCCLRRGKQKSTKVEEGSKAAAVDKLLAGDEIVGINDVGLSGFRQEAICLVKGSHKTLKLVVKRRNELNWRPHSWHATKFSDSQSEPAAPLLPCPSRPSRYHASSSSQDLSGGAWEPANLQRTSDHFSSLGSVDSLDPSSQPDPSGRLSAAKSSSSIDRLGGASKRDSAYGSFSTRSSTPDHTLPKADASSAENILYKVGLWEASAAADRRPGLAAGDPQGTEEPLGCFPPRVPEDGPEPKLAACGRSSSGPVWYVPDKRRAPPSPPPPPPPLRRDSFAATRIHEQTQGPTVSADAAPAQPLPGLSCVQPHGGDWKRPEPADQLLRPARGADGRRAASSGCAPGAHVDCGWPACDSGSRALLGAPSRLQASLSSTDVRRPQPSPVCQHPRHLSDEGPFFRKGPRATTWLGEPLPADCLGDNTPAAIVIGGPRAADQRGDCSAQSRYYCVMSRPGPQWGAPAPQPRCYPSRLEGAPGARQRSRADPVDRAPEDPPGAGLLDRGGRKRVVGSLDDGGGEICPLQTPMLHSLSRERACPPETRHKDMAPAPPEALAGKPLRRSDRFATTLRNEIQQRRARLQKSRSTATLASSAEEEGEVEDDVETEARGSWAQPVGAPETTFPSGTYKDHLKEAQARVLRATSFRRRDLDLSLADPEPAPRVWEGAPGARPPLCAAGSGSGSGSARIGSRRRFTAEQKLKSYSEPEKMNAVGLTGDQSPAPHDETLWTFADRWKFFEETSKPVGPQPGPRPAPCGFPREKPEMLWTVGPGKDGKAGRPIPPERLGTFAEYQASWREQRKVPEARGGGRYHSTDNILDVGVDQEERPQYVHERSRSSPSTDLYKQDASMEPRWEAGDPEEHTELSSAAPAEDRSPTPRQADGQRSEDHPGALRDPRQTSEPPGSREGPEVSLEARGRAGTLPRDYRYLEPRTPLEPPAQGQEPRPLSAAATARRPALQRPQPPKRELWPPPGDTPSRPHTSVASSEAVDTRSTRRVEEPTARPWREALLPAPFRPLQTSAMDSSRSPSPQFAPQKLTDKPPLLIQDENAARIERVMDNNTTVKMVPIKIVHSESQPEKESRQALARVPEPPPLPRGLERDQIKTLSTSEQSYSRFCPYSRQGAEPQPATGALGPPAKEGRASPPVLSYVKAKDRTADDLKSEELAREIAGRDKSLADILDPGVRMRTTMDLMEGIFPKDEHLLEGAQQRRKLLPKGPSPRTAEERREETSVLAAAPLATNSAYYSTSAPKAELLIKMKDLQEQQEPEEDSGSDLDHDLSVKKQELIESLGRKLQVLREARESLLEDVQANSALGDEVEALVKAVCKPNEFDKFRMFIGDLDKVVNLLLSLSGRLARVENALNNLDDSTPPGDRQSLLEKQRVLIQQHEDARELKENLDRREGIVFDILVSYLGPESLADYEHFVKMKSALIIEQRELEDKIHLGEEQLKCLLDSLQPERGK

Target ORF information:

RefSeq Version	XM_024988425.1
Organism	Bos taurus(cattle)
Definition	Bos taurus shroom family member 2 (SHROOM2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988425.1

ORF Insert Sequence:

ATGCCCCACC TTGAGCCAGG TGACCGTTCG GTAGGCTGGC CTCAGAGAAA ACTCTGCAGG 
CTGCCTGCCA AGTCAGATCT TTGTCGTTCC TCCCTGGCTC CTGCAGTCTT TCTCTCTGGA 
GTCTCTGTGT GCAGACAGCG CTGCTGTCTC CGGCGTGGGA AACAGAAAAG TACCAAGGTT 
GAAGAAGGCA GCAAAGCCGC GGCCGTGGAC AAACTGCTGG CCGGAGACGA GATCGTGGGC 
ATCAATGATG TCGGTCTGTC CGGGTTTAGA CAGGAAGCCA TCTGCCTGGT GAAGGGGTCA 
CATAAGACCC TGAAGCTGGT GGTGAAAAGG AGGAATGAAC TGAACTGGAG GCCACACTCC 
TGGCATGCCA CCAAGTTCTC TGACAGCCAG TCTGAGCCAG CTGCACCTCT GCTTCCCTGC 
CCCTCTCGGC CGAGCCGGTA CCACGCCAGC TCCTCCTCCC AGGACCTGTC GGGCGGTGCC 
TGGGAGCCAG CGAACCTGCA GCGCACCTCG GACCACTTCA GCTCGCTGGG GAGCGTCGAC 
AGCCTGGACC CATCCTCCCA GCCCGACCCG TCGGGGCGCC TCTCCGCGGC CAAGTCCAGC 
AGCAGCATCG ACCGCCTGGG CGGTGCCAGC AAGCGGGACT CAGCTTATGG TTCATTCTCC 
ACGCGCTCCA GCACCCCGGA CCACACCCTG CCCAAGGCAG ACGCGTCCTC GGCCGAGAAT 
ATCCTCTACA AAGTGGGCCT GTGGGAGGCC TCCGCTGCAG CGGACAGACG GCCGGGCCTG 
GCGGCTGGCG ACCCTCAGGG CACCGAGGAG CCCCTGGGGT GCTTCCCACC CCGGGTCCCT 
GAGGACGGCC CCGAGCCCAA GCTAGCTGCT TGCGGGCGGT CCAGCTCCGG GCCCGTCTGG 
TATGTTCCGG ATAAGAGAAG AGCCCCTCCG TCCCCTCCCC CGCCGCCTCC CCCGCTCCGC 
CGTGACAGCT TCGCTGCCAC CAGGATCCAC GAGCAGACCC AGGGCCCCAC GGTCTCAGCA 
GACGCAGCCC CAGCGCAGCC CTTGCCGGGC CTGAGCTGCG TGCAGCCCCA CGGTGGCGAC 
TGGAAGAGAC CTGAACCCGC TGATCAGCTG TTGAGGCCGG CGCGCGGGGC TGACGGCCGG 
AGAGCTGCGA GCTCGGGGTG CGCTCCAGGG GCACATGTGG ACTGTGGGTG GCCGGCCTGC 
GACAGCGGGT CCCGGGCCCT CTTGGGGGCC CCCAGCCGGC TGCAGGCTTC TCTCTCGAGC 
ACTGACGTGC GCCGCCCGCA GCCCTCGCCC GTGTGCCAAC ACCCGCGCCA CTTGAGCGAC 
GAGGGCCCCT TCTTCCGCAA GGGCCCCAGG GCCACCACGT GGCTAGGGGA GCCCCTCCCC 
GCTGACTGCC TCGGGGACAA CACCCCTGCT GCCATTGTCA TCGGAGGGCC ACGCGCTGCA 
GACCAGAGGG GCGACTGCAG CGCGCAGAGC CGCTACTACT GCGTGATGTC CAGACCCGGT 
CCCCAGTGGG GCGCCCCGGC CCCTCAGCCC CGCTGCTACC CGTCCCGGCT GGAGGGCGCC 
CCGGGCGCAC GGCAGAGAAG CAGGGCAGAC CCGGTGGACA GGGCACCCGA GGACCCACCG 
GGGGCTGGCC TCCTGGACAG AGGTGGCAGG AAGAGGGTGG TGGGCAGCTT GGACGACGGG 
GGCGGCGAGA TCTGCCCCCT GCAGACGCCC ATGCTGCACT CGCTGAGCCG GGAAAGGGCG 
TGCCCGCCCG AGACCCGCCA CAAGGACATG GCGCCGGCGC CGCCTGAGGC CCTGGCAGGC 
AAGCCGCTCC GGAGGAGTGA CCGCTTCGCC ACCACCCTGA GGAATGAGAT CCAGCAGCGG 
CGAGCCAGGC TGCAGAAGAG CCGGAGCACG GCCACCTTGG CCAGCTCCGC GGAGGAGGAG 
GGGGAGGTGG AAGACGACGT GGAGACTGAG GCGCGCGGCT CCTGGGCGCA GCCAGTGGGC 
GCCCCGGAGA CCACCTTCCC CAGCGGCACC TATAAGGACC ACTTGAAGGA GGCGCAGGCC 
CGCGTCCTGA GGGCCACGTC GTTCCGGCGC CGTGACCTGG ATCTCAGCCT GGCGGACCCG 
GAGCCAGCCC CCCGCGTCTG GGAGGGGGCC CCGGGTGCCA GGCCGCCCTT GTGCGCTGCC 
GGCAGTGGCA GTGGCAGCGG CAGCGCGCGC ATCGGGAGCC GGAGGCGCTT CACTGCCGAG 
CAGAAACTGA AGTCCTACTC GGAGCCCGAG AAGATGAACG CGGTGGGGCT GACGGGGGAC 
CAGAGCCCTG CACCCCATGA TGAGACGCTG TGGACCTTTG CTGACAGGTG GAAGTTTTTT 
GAGGAAACCA GCAAGCCCGT GGGCCCCCAG CCCGGGCCGA GGCCGGCGCC CTGTGGCTTC 
CCCAGGGAGA AGCCGGAGAT GCTGTGGACA GTGGGCCCTG GAAAAGACGG AAAGGCGGGG 
AGACCCATCC CGCCAGAGCG GCTCGGCACC TTCGCAGAGT ATCAAGCCTC CTGGAGGGAA 
CAGAGGAAAG TCCCGGAAGC CAGGGGTGGC GGGAGGTACC ACTCCACTGA CAACATCCTC 
GACGTGGGTG TGGACCAGGA AGAGAGGCCA CAGTACGTCC ACGAAAGGTC CCGGTCATCA 
CCTTCCACGG ACCTCTACAA GCAGGACGCC TCCATGGAAC CTCGATGGGA AGCAGGGGAC 
CCGGAGGAGC ACACGGAGCT TTCTTCGGCG GCACCGGCCG AGGACAGAAG CCCCACCCCA 
AGACAAGCAG ATGGTCAGCG TTCGGAGGAC CACCCAGGAG CACTGCGGGA CCCCCGGCAA 
ACCTCTGAAC CCCCTGGCAG CAGGGAAGGG CCCGAGGTGT CCCTGGAGGC CCGCGGCCGG 
GCGGGCACTC TCCCTCGCGA TTACCGGTAC CTGGAGCCGC GCACGCCCCT GGAGCCACCC 
GCCCAGGGGC AGGAGCCCCG GCCCCTGAGC GCCGCCGCGA CAGCCCGGAG ACCCGCCCTA 
CAGAGGCCAC AGCCGCCCAA GCGCGAGCTC TGGCCGCCGC CAGGGGACAC GCCCAGCCGG 
CCTCACACCT CCGTGGCCTC CTCGGAGGCG GTGGACACGC GGAGCACGCG GCGCGTGGAG 
GAGCCCACGG CCCGGCCCTG GCGGGAGGCG CTGCTTCCCG CCCCGTTCCG GCCCCTGCAG 
ACCTCCGCCA TGGACAGCTC GCGCTCGCCG TCGCCGCAGT TCGCGCCGCA GAAGCTGACG 
GACAAGCCCC CTCTGCTCAT CCAGGACGAG AATGCTGCCA GAATCGAGCG GGTGATGGAC 
AACAACACCA CGGTGAAGAT GGTGCCCATC AAGATAGTGC ATTCGGAGAG CCAGCCGGAG 
AAGGAGAGCC GCCAGGCACT GGCCCGCGTC CCCGAGCCAC CTCCGCTGCC CCGGGGCCTC 
GAGCGGGACC AGATCAAGAC GCTCAGCACG TCTGAGCAGT CCTACTCACG CTTCTGCCCC 
TACAGCCGCC AGGGTGCCGA GCCCCAGCCC GCCACCGGCG CCCTGGGGCC CCCCGCCAAG 
GAAGGCCGGG CCTCCCCTCC TGTGCTCAGC TACGTGAAGG CCAAAGACAG GACCGCCGAC 
GACCTCAAGT CAGAGGAGCT GGCCCGCGAG ATCGCGGGCA GGGATAAGTC GCTGGCGGAC 
ATCCTAGACC CGGGCGTGAG GATGAGAACC ACCATGGACC TCATGGAGGG CATCTTCCCC 
AAAGACGAGC ACCTCCTGGA AGGCGCACAG CAACGGAGGA AGCTGCTGCC CAAGGGGCCC 
TCGCCCAGGA CGGCGGAGGA GAGGAGGGAG GAGACCAGTG TGCTGGCAGC CGCACCCTTG 
GCCACCAACT CCGCCTACTA CAGCACGTCA GCTCCCAAGG CGGAGCTGCT GATAAAGATG 
AAGGACCTGC AGGAGCAGCA GGAGCCAGAG GAGGACTCAG GCAGTGACTT GGACCACGAC 
CTGTCAGTCA AAAAGCAGGA GCTCATTGAG AGCCTTGGCC GCAAGCTGCA GGTGCTGCGG 
GAGGCGCGGG AGAGCCTGCT GGAGGACGTG CAGGCCAACA GCGCGCTGGG CGACGAGGTG 
GAGGCCCTGG TCAAGGCCGT GTGCAAGCCC AATGAGTTTG ACAAGTTCCG CATGTTCATC 
GGGGACCTCG ACAAGGTGGT GAACCTCCTG CTGTCGCTGT CCGGCCGCCT GGCCCGCGTG 
GAGAACGCCC TCAACAACCT GGACGACAGC ACGCCTCCGG GCGATCGGCA ATCCCTGCTC 
GAGAAGCAGC GCGTCCTGAT CCAGCAGCAC GAAGACGCCA GGGAGCTGAA GGAGAACCTG 
GACCGCCGGG AAGGCATCGT CTTCGACATC CTGGTCAGCT ACCTGGGCCC CGAGAGCCTG 
GCGGACTACG AGCACTTCGT CAAGATGAAG TCGGCGCTCA TCATCGAACA GCGCGAGCTG 
GAAGACAAAA TACACCTGGG TGAGGAGCAG CTGAAGTGCC TCTTGGACAG CCTGCAGCCC 
GAGAGAGGCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHROOM2 ( XM_024988426.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844194.1 Bos taurus shroom family member 2 (SHROOM2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa238590
Clone ID Related Accession (Same CDS sequence)	XM_024988426.1
Accession Version	XM_024988426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein Shroom2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCACC TTGAGCCAGG TGACCGTTCG GTAGGCTGGC CTCAGAGAAA ACTCTGCAGG 
CTGCCTGCCA AGTCAGATCT TTGTCGTTCC TCCCTGGCTC CTGCAGTCTT TCTCTCTGGA 
GTCTCTGTGT GCAGACAGCG CTGCTGTCTC CGGCGTGGGA AACAGAAAAG TACCAAGGTT 
GAAGAAGGCA GCAAAGCCGC GGCCGTGGAC AAACTGCTGG CCGGAGACGA GATCGTGGGC 
ATCAATGATG TCGGTCTGTC CGGGTTTAGA CAGGAAGCCA TCTGCCTGGT GAAGGGGTCA 
CATAAGACCC TGAAGCTGGT GGTGAAAAGG AGGAATGAAC TGAACTGGAG GCCACACTCC 
TGGCATGCCA CCAAGTTCTC TGACAGCCAG TCTGAGCCAG CTGCACCTCT GCTTCCCTGC 
CCCTCTCGGC CGAGCCGGTA CCACGCCAGC TCCTCCTCCC AGGACCTGTC GGGCGGTGCC 
TGGGAGCCAG CGAACCTGCA GCGCACCTCG GACCACTTCA GCTCGCTGGG GAGCGTCGAC 
AGCCTGGACC CATCCTCCCA GCCCGACCCG TCGGGGCGCC TCTCCGCGGC CAAGTCCAGC 
AGCAGCATCG ACCGCCTGGG CGGTGCCAGC AAGCGGGACT CAGCTTATGG TTCATTCTCC 
ACGCGCTCCA GCACCCCGGA CCACACCCTG CCCAAGGCAG ACGCGTCCTC GGCCGAGAAT 
ATCCTCTACA AAGTGGGCCT GTGGGAGGCC TCCGCTGCAG CGGACAGACG GCCGGGCCTG 
GCGGCTGGCG ACCCTCAGGG CACCGAGGAG CCCCTGGGGT GCTTCCCACC CCGGGTCCCT 
GAGGACGGCC CCGAGCCCAA GCTAGCTGCT TGCGGGCGGT CCAGCTCCGG GCCCGTCTGG 
TATGTTCCGG ATAAGAGAAG AGCCCCTCCG TCCCCTCCCC CGCCGCCTCC CCCGCTCCGC 
CGTGACAGCT TCGCTGCCAC CAGGATCCAC GAGCAGACCC AGGGCCCCAC GGTCTCAGCA 
GACGCAGCCC CAGCGCAGCC CTTGCCGGGC CTGAGCTGCG TGCAGCCCCA CGGTGGCGAC 
TGGAAGAGAC CTGAACCCGC TGATCAGCTG TTGAGGCCGG CGCGCGGGGC TGACGGCCGG 
AGAGCTGCGA GCTCGGGGTG CGCTCCAGGG GCACATGTGG ACTGTGGGTG GCCGGCCTGC 
GACAGCGGGT CCCGGGCCCT CTTGGGGGCC CCCAGCCGGC TGCAGGCTTC TCTCTCGAGC 
ACTGACGTGC GCCGCCCGCA GCCCTCGCCC GTGTGCCAAC ACCCGCGCCA CTTGAGCGAC 
GAGGGCCCCT TCTTCCGCAA GGGCCCCAGG GCCACCACGT GGCTAGGGGA GCCCCTCCCC 
GCTGACTGCC TCGGGGACAA CACCCCTGCT GCCATTGTCA TCGGAGGGCC ACGCGCTGCA 
GACCAGAGGG GCGACTGCAG CGCGCAGAGC CGCTACTACT GCGTGATGTC CAGACCCGGT 
CCCCAGTGGG GCGCCCCGGC CCCTCAGCCC CGCTGCTACC CGTCCCGGCT GGAGGGCGCC 
CCGGGCGCAC GGCAGAGAAG CAGGGCAGAC CCGGTGGACA GGGCACCCGA GGACCCACCG 
GGGGCTGGCC TCCTGGACAG AGGTGGCAGG AAGAGGGTGG TGGGCAGCTT GGACGACGGG 
GGCGGCGAGA TCTGCCCCCT GCAGACGCCC ATGCTGCACT CGCTGAGCCG GGAAAGGGCG 
TGCCCGCCCG AGACCCGCCA CAAGGACATG GCGCCGGCGC CGCCTGAGGC CCTGGCAGGC 
AAGCCGCTCC GGAGGAGTGA CCGCTTCGCC ACCACCCTGA GGAATGAGAT CCAGCAGCGG 
CGAGCCAGGC TGCAGAAGAG CCGGAGCACG GCCACCTTGG CCAGCTCCGC GGAGGAGGAG 
GGGGAGGTGG AAGACGACGT GGAGACTGAG GCGCGCGGCT CCTGGGCGCA GCCAGTGGGC 
GCCCCGGAGA CCACCTTCCC CAGCGGCACC TATAAGGACC ACTTGAAGGA GGCGCAGGCC 
CGCGTCCTGA GGGCCACGTC GTTCCGGCGC CGTGACCTGG ATCTCAGCCT GGCGGACCCG 
GAGCCAGCCC CCCGCGTCTG GGAGGGGGCC CCGGGTGCCA GGCCGCCCTT GTGCGCTGCC 
GGCAGTGGCA GTGGCAGCGG CAGCGCGCGC ATCGGGAGCC GGAGGCGCTT CACTGCCGAG 
CAGAAACTGA AGTCCTACTC GGAGCCCGAG AAGATGAACG CGGTGGGGCT GACGGGGGAC 
CAGAGCCCTG CACCCCATGA TGAGACGCTG TGGACCTTTG CTGACAGGTG GAAGTTTTTT 
GAGGAAACCA GCAAGCCCGT GGGCCCCCAG CCCGGGCCGA GGCCGGCGCC CTGTGGCTTC 
CCCAGGGAGA AGCCGGAGAT GCTGTGGACA GTGGGCCCTG GAAAAGACGG AAAGGCGGGG 
AGACCCATCC CGCCAGAGCG GCTCGGCACC TTCGCAGAGT ATCAAGCCTC CTGGAGGGAA 
CAGAGGAAAG TCCCGGAAGC CAGGGGTGGC GGGAGGTACC ACTCCACTGA CAACATCCTC 
GACGTGGGTG TGGACCAGGA AGAGAGGCCA CAGTACGTCC ACGAAAGGTC CCGGTCATCA 
CCTTCCACGG ACCTCTACAA GCAGGACGCC TCCATGGAAC CTCGATGGGA AGCAGGGGAC 
CCGGAGGAGC ACACGGAGCT TTCTTCGGCG GCACCGGCCG AGGACAGAAG CCCCACCCCA 
AGACAAGCAG ATGGTCAGCG TTCGGAGGAC CACCCAGGAG CACTGCGGGA CCCCCGGCAA 
ACCTCTGAAC CCCCTGGCAG CAGGGAAGGG CCCGAGGTGT CCCTGGAGGC CCGCGGCCGG 
GCGGGCACTC TCCCTCGCGA TTACCGGTAC CTGGAGCCGC GCACGCCCCT GGAGCCACCC 
GCCCAGGGGC AGGAGCCCCG GCCCCTGAGC GCCGCCGCGA CAGCCCGGAG ACCCGCCCTA 
CAGAGGCCAC AGCCGCCCAA GCGCGAGCTC TGGCCGCCGC CAGGGGACAC GCCCAGCCGG 
CCTCACACCT CCGTGGCCTC CTCGGAGGCG GTGGACACGC GGAGCACGCG GCGCGTGGAG 
GAGCCCACGG CCCGGCCCTG GCGGGAGGCG CTGCTTCCCG CCCCGTTCCG GCCCCTGCAG 
ACCTCCGCCA TGGACAGCTC GCGCTCGCCG TCGCCGCAGT TCGCGCCGCA GAAGCTGACG 
GACAAGCCCC CTCTGCTCAT CCAGGACGAG AATGCTGCCA GAATCGAGCG GGTGATGGAC 
AACAACACCA CGGTGAAGAT GGTGCCCATC AAGATAGTGC ATTCGGAGAG CCAGCCGGAG 
AAGGAGAGCC GCCAGGCACT GGCCCGCGTC CCCGAGCCAC CTCCGCTGCC CCGGGGCCTC 
GAGCGGGACC AGATCAAGAC GCTCAGCACG TCTGAGCAGT CCTACTCACG CTTCTGCCCC 
TACAGCCGCC AGGGTGCCGA GCCCCAGCCC GCCACCGGCG CCCTGGGGCC CCCCGCCAAG 
GAAGGCCGGG CCTCCCCTCC TGTGCTCAGC TACGTGAAGG CCAAAGACAG GACCGCCGAC 
GACCTCAAGT CAGAGGAGCT GGCCCGCGAG ATCGCGGGCA GGGATAAGTC GCTGGCGGAC 
ATCCTAGACC CGGGCGTGAG GATGAGAACC ACCATGGACC TCATGGAGGG CATCTTCCCC 
AAAGACGAGC ACCTCCTGGA AGGCGCACAG CAACGGAGGA AGCTGCTGCC CAAGGGGCCC 
TCGCCCAGGA CGGCGGAGGA GAGGAGGGAG GAGACCAGTG TGCTGGCAGC CGCACCCTTG 
GCCACCAACT CCGCCTACTA CAGCACGTCA GCTCCCAAGG CGGAGCTGCT GATAAAGATG 
AAGGACCTGC AGGAGCAGCA GGAGCCAGAG GAGGACTCAG GCAGTGACTT GGACCACGAC 
CTGTCAGTCA AAAAGGAGCT CATTGAGAGC CTTGGCCGCA AGCTGCAGGT GCTGCGGGAG 
GCGCGGGAGA GCCTGCTGGA GGACGTGCAG GCCAACAGCG CGCTGGGCGA CGAGGTGGAG 
GCCCTGGTCA AGGCCGTGTG CAAGCCCAAT GAGTTTGACA AGTTCCGCAT GTTCATCGGG 
GACCTCGACA AGGTGGTGAA CCTCCTGCTG TCGCTGTCCG GCCGCCTGGC CCGCGTGGAG 
AACGCCCTCA ACAACCTGGA CGACAGCACG CCTCCGGGCG ATCGGCAATC CCTGCTCGAG 
AAGCAGCGCG TCCTGATCCA GCAGCACGAA GACGCCAGGG AGCTGAAGGA GAACCTGGAC 
CGCCGGGAAG GCATCGTCTT CGACATCCTG GTCAGCTACC TGGGCCCCGA GAGCCTGGCG 
GACTACGAGC ACTTCGTCAA GATGAAGTCG GCGCTCATCA TCGAACAGCG CGAGCTGGAA 
GACAAAATAC ACCTGGGTGA GGAGCAGCTG AAGTGCCTCT TGGACAGCCT GCAGCCCGAG 
AGAGGCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844194.1
CDS	325..4836
Translation	MPHLEPGDRSVGWPQRKLCRLPAKSDLCRSSLAPAVFLSGVSVCRQRCCLRRGKQKSTKVEEGSKAAAVDKLLAGDEIVGINDVGLSGFRQEAICLVKGSHKTLKLVVKRRNELNWRPHSWHATKFSDSQSEPAAPLLPCPSRPSRYHASSSSQDLSGGAWEPANLQRTSDHFSSLGSVDSLDPSSQPDPSGRLSAAKSSSSIDRLGGASKRDSAYGSFSTRSSTPDHTLPKADASSAENILYKVGLWEASAAADRRPGLAAGDPQGTEEPLGCFPPRVPEDGPEPKLAACGRSSSGPVWYVPDKRRAPPSPPPPPPPLRRDSFAATRIHEQTQGPTVSADAAPAQPLPGLSCVQPHGGDWKRPEPADQLLRPARGADGRRAASSGCAPGAHVDCGWPACDSGSRALLGAPSRLQASLSSTDVRRPQPSPVCQHPRHLSDEGPFFRKGPRATTWLGEPLPADCLGDNTPAAIVIGGPRAADQRGDCSAQSRYYCVMSRPGPQWGAPAPQPRCYPSRLEGAPGARQRSRADPVDRAPEDPPGAGLLDRGGRKRVVGSLDDGGGEICPLQTPMLHSLSRERACPPETRHKDMAPAPPEALAGKPLRRSDRFATTLRNEIQQRRARLQKSRSTATLASSAEEEGEVEDDVETEARGSWAQPVGAPETTFPSGTYKDHLKEAQARVLRATSFRRRDLDLSLADPEPAPRVWEGAPGARPPLCAAGSGSGSGSARIGSRRRFTAEQKLKSYSEPEKMNAVGLTGDQSPAPHDETLWTFADRWKFFEETSKPVGPQPGPRPAPCGFPREKPEMLWTVGPGKDGKAGRPIPPERLGTFAEYQASWREQRKVPEARGGGRYHSTDNILDVGVDQEERPQYVHERSRSSPSTDLYKQDASMEPRWEAGDPEEHTELSSAAPAEDRSPTPRQADGQRSEDHPGALRDPRQTSEPPGSREGPEVSLEARGRAGTLPRDYRYLEPRTPLEPPAQGQEPRPLSAAATARRPALQRPQPPKRELWPPPGDTPSRPHTSVASSEAVDTRSTRRVEEPTARPWREALLPAPFRPLQTSAMDSSRSPSPQFAPQKLTDKPPLLIQDENAARIERVMDNNTTVKMVPIKIVHSESQPEKESRQALARVPEPPPLPRGLERDQIKTLSTSEQSYSRFCPYSRQGAEPQPATGALGPPAKEGRASPPVLSYVKAKDRTADDLKSEELAREIAGRDKSLADILDPGVRMRTTMDLMEGIFPKDEHLLEGAQQRRKLLPKGPSPRTAEERREETSVLAAAPLATNSAYYSTSAPKAELLIKMKDLQEQQEPEEDSGSDLDHDLSVKKELIESLGRKLQVLREARESLLEDVQANSALGDEVEALVKAVCKPNEFDKFRMFIGDLDKVVNLLLSLSGRLARVENALNNLDDSTPPGDRQSLLEKQRVLIQQHEDARELKENLDRREGIVFDILVSYLGPESLADYEHFVKMKSALIIEQRELEDKIHLGEEQLKCLLDSLQPERGK

Target ORF information:

RefSeq Version	XM_024988426.1
Organism	Bos taurus(cattle)
Definition	Bos taurus shroom family member 2 (SHROOM2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988426.1

ORF Insert Sequence:

ATGCCCCACC TTGAGCCAGG TGACCGTTCG GTAGGCTGGC CTCAGAGAAA ACTCTGCAGG 
CTGCCTGCCA AGTCAGATCT TTGTCGTTCC TCCCTGGCTC CTGCAGTCTT TCTCTCTGGA 
GTCTCTGTGT GCAGACAGCG CTGCTGTCTC CGGCGTGGGA AACAGAAAAG TACCAAGGTT 
GAAGAAGGCA GCAAAGCCGC GGCCGTGGAC AAACTGCTGG CCGGAGACGA GATCGTGGGC 
ATCAATGATG TCGGTCTGTC CGGGTTTAGA CAGGAAGCCA TCTGCCTGGT GAAGGGGTCA 
CATAAGACCC TGAAGCTGGT GGTGAAAAGG AGGAATGAAC TGAACTGGAG GCCACACTCC 
TGGCATGCCA CCAAGTTCTC TGACAGCCAG TCTGAGCCAG CTGCACCTCT GCTTCCCTGC 
CCCTCTCGGC CGAGCCGGTA CCACGCCAGC TCCTCCTCCC AGGACCTGTC GGGCGGTGCC 
TGGGAGCCAG CGAACCTGCA GCGCACCTCG GACCACTTCA GCTCGCTGGG GAGCGTCGAC 
AGCCTGGACC CATCCTCCCA GCCCGACCCG TCGGGGCGCC TCTCCGCGGC CAAGTCCAGC 
AGCAGCATCG ACCGCCTGGG CGGTGCCAGC AAGCGGGACT CAGCTTATGG TTCATTCTCC 
ACGCGCTCCA GCACCCCGGA CCACACCCTG CCCAAGGCAG ACGCGTCCTC GGCCGAGAAT 
ATCCTCTACA AAGTGGGCCT GTGGGAGGCC TCCGCTGCAG CGGACAGACG GCCGGGCCTG 
GCGGCTGGCG ACCCTCAGGG CACCGAGGAG CCCCTGGGGT GCTTCCCACC CCGGGTCCCT 
GAGGACGGCC CCGAGCCCAA GCTAGCTGCT TGCGGGCGGT CCAGCTCCGG GCCCGTCTGG 
TATGTTCCGG ATAAGAGAAG AGCCCCTCCG TCCCCTCCCC CGCCGCCTCC CCCGCTCCGC 
CGTGACAGCT TCGCTGCCAC CAGGATCCAC GAGCAGACCC AGGGCCCCAC GGTCTCAGCA 
GACGCAGCCC CAGCGCAGCC CTTGCCGGGC CTGAGCTGCG TGCAGCCCCA CGGTGGCGAC 
TGGAAGAGAC CTGAACCCGC TGATCAGCTG TTGAGGCCGG CGCGCGGGGC TGACGGCCGG 
AGAGCTGCGA GCTCGGGGTG CGCTCCAGGG GCACATGTGG ACTGTGGGTG GCCGGCCTGC 
GACAGCGGGT CCCGGGCCCT CTTGGGGGCC CCCAGCCGGC TGCAGGCTTC TCTCTCGAGC 
ACTGACGTGC GCCGCCCGCA GCCCTCGCCC GTGTGCCAAC ACCCGCGCCA CTTGAGCGAC 
GAGGGCCCCT TCTTCCGCAA GGGCCCCAGG GCCACCACGT GGCTAGGGGA GCCCCTCCCC 
GCTGACTGCC TCGGGGACAA CACCCCTGCT GCCATTGTCA TCGGAGGGCC ACGCGCTGCA 
GACCAGAGGG GCGACTGCAG CGCGCAGAGC CGCTACTACT GCGTGATGTC CAGACCCGGT 
CCCCAGTGGG GCGCCCCGGC CCCTCAGCCC CGCTGCTACC CGTCCCGGCT GGAGGGCGCC 
CCGGGCGCAC GGCAGAGAAG CAGGGCAGAC CCGGTGGACA GGGCACCCGA GGACCCACCG 
GGGGCTGGCC TCCTGGACAG AGGTGGCAGG AAGAGGGTGG TGGGCAGCTT GGACGACGGG 
GGCGGCGAGA TCTGCCCCCT GCAGACGCCC ATGCTGCACT CGCTGAGCCG GGAAAGGGCG 
TGCCCGCCCG AGACCCGCCA CAAGGACATG GCGCCGGCGC CGCCTGAGGC CCTGGCAGGC 
AAGCCGCTCC GGAGGAGTGA CCGCTTCGCC ACCACCCTGA GGAATGAGAT CCAGCAGCGG 
CGAGCCAGGC TGCAGAAGAG CCGGAGCACG GCCACCTTGG CCAGCTCCGC GGAGGAGGAG 
GGGGAGGTGG AAGACGACGT GGAGACTGAG GCGCGCGGCT CCTGGGCGCA GCCAGTGGGC 
GCCCCGGAGA CCACCTTCCC CAGCGGCACC TATAAGGACC ACTTGAAGGA GGCGCAGGCC 
CGCGTCCTGA GGGCCACGTC GTTCCGGCGC CGTGACCTGG ATCTCAGCCT GGCGGACCCG 
GAGCCAGCCC CCCGCGTCTG GGAGGGGGCC CCGGGTGCCA GGCCGCCCTT GTGCGCTGCC 
GGCAGTGGCA GTGGCAGCGG CAGCGCGCGC ATCGGGAGCC GGAGGCGCTT CACTGCCGAG 
CAGAAACTGA AGTCCTACTC GGAGCCCGAG AAGATGAACG CGGTGGGGCT GACGGGGGAC 
CAGAGCCCTG CACCCCATGA TGAGACGCTG TGGACCTTTG CTGACAGGTG GAAGTTTTTT 
GAGGAAACCA GCAAGCCCGT GGGCCCCCAG CCCGGGCCGA GGCCGGCGCC CTGTGGCTTC 
CCCAGGGAGA AGCCGGAGAT GCTGTGGACA GTGGGCCCTG GAAAAGACGG AAAGGCGGGG 
AGACCCATCC CGCCAGAGCG GCTCGGCACC TTCGCAGAGT ATCAAGCCTC CTGGAGGGAA 
CAGAGGAAAG TCCCGGAAGC CAGGGGTGGC GGGAGGTACC ACTCCACTGA CAACATCCTC 
GACGTGGGTG TGGACCAGGA AGAGAGGCCA CAGTACGTCC ACGAAAGGTC CCGGTCATCA 
CCTTCCACGG ACCTCTACAA GCAGGACGCC TCCATGGAAC CTCGATGGGA AGCAGGGGAC 
CCGGAGGAGC ACACGGAGCT TTCTTCGGCG GCACCGGCCG AGGACAGAAG CCCCACCCCA 
AGACAAGCAG ATGGTCAGCG TTCGGAGGAC CACCCAGGAG CACTGCGGGA CCCCCGGCAA 
ACCTCTGAAC CCCCTGGCAG CAGGGAAGGG CCCGAGGTGT CCCTGGAGGC CCGCGGCCGG 
GCGGGCACTC TCCCTCGCGA TTACCGGTAC CTGGAGCCGC GCACGCCCCT GGAGCCACCC 
GCCCAGGGGC AGGAGCCCCG GCCCCTGAGC GCCGCCGCGA CAGCCCGGAG ACCCGCCCTA 
CAGAGGCCAC AGCCGCCCAA GCGCGAGCTC TGGCCGCCGC CAGGGGACAC GCCCAGCCGG 
CCTCACACCT CCGTGGCCTC CTCGGAGGCG GTGGACACGC GGAGCACGCG GCGCGTGGAG 
GAGCCCACGG CCCGGCCCTG GCGGGAGGCG CTGCTTCCCG CCCCGTTCCG GCCCCTGCAG 
ACCTCCGCCA TGGACAGCTC GCGCTCGCCG TCGCCGCAGT TCGCGCCGCA GAAGCTGACG 
GACAAGCCCC CTCTGCTCAT CCAGGACGAG AATGCTGCCA GAATCGAGCG GGTGATGGAC 
AACAACACCA CGGTGAAGAT GGTGCCCATC AAGATAGTGC ATTCGGAGAG CCAGCCGGAG 
AAGGAGAGCC GCCAGGCACT GGCCCGCGTC CCCGAGCCAC CTCCGCTGCC CCGGGGCCTC 
GAGCGGGACC AGATCAAGAC GCTCAGCACG TCTGAGCAGT CCTACTCACG CTTCTGCCCC 
TACAGCCGCC AGGGTGCCGA GCCCCAGCCC GCCACCGGCG CCCTGGGGCC CCCCGCCAAG 
GAAGGCCGGG CCTCCCCTCC TGTGCTCAGC TACGTGAAGG CCAAAGACAG GACCGCCGAC 
GACCTCAAGT CAGAGGAGCT GGCCCGCGAG ATCGCGGGCA GGGATAAGTC GCTGGCGGAC 
ATCCTAGACC CGGGCGTGAG GATGAGAACC ACCATGGACC TCATGGAGGG CATCTTCCCC 
AAAGACGAGC ACCTCCTGGA AGGCGCACAG CAACGGAGGA AGCTGCTGCC CAAGGGGCCC 
TCGCCCAGGA CGGCGGAGGA GAGGAGGGAG GAGACCAGTG TGCTGGCAGC CGCACCCTTG 
GCCACCAACT CCGCCTACTA CAGCACGTCA GCTCCCAAGG CGGAGCTGCT GATAAAGATG 
AAGGACCTGC AGGAGCAGCA GGAGCCAGAG GAGGACTCAG GCAGTGACTT GGACCACGAC 
CTGTCAGTCA AAAAGGAGCT CATTGAGAGC CTTGGCCGCA AGCTGCAGGT GCTGCGGGAG 
GCGCGGGAGA GCCTGCTGGA GGACGTGCAG GCCAACAGCG CGCTGGGCGA CGAGGTGGAG 
GCCCTGGTCA AGGCCGTGTG CAAGCCCAAT GAGTTTGACA AGTTCCGCAT GTTCATCGGG 
GACCTCGACA AGGTGGTGAA CCTCCTGCTG TCGCTGTCCG GCCGCCTGGC CCGCGTGGAG 
AACGCCCTCA ACAACCTGGA CGACAGCACG CCTCCGGGCG ATCGGCAATC CCTGCTCGAG 
AAGCAGCGCG TCCTGATCCA GCAGCACGAA GACGCCAGGG AGCTGAAGGA GAACCTGGAC 
CGCCGGGAAG GCATCGTCTT CGACATCCTG GTCAGCTACC TGGGCCCCGA GAGCCTGGCG 
GACTACGAGC ACTTCGTCAA GATGAAGTCG GCGCTCATCA TCGAACAGCG CGAGCTGGAA 
GACAAAATAC ACCTGGGTGA GGAGCAGCTG AAGTGCCTCT TGGACAGCCT GCAGCCCGAG 
AGAGGCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHROOM2 ( XM_002700461.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700507.3 Bos taurus shroom family member 2 (SHROOM2), transcript variant X3, mRNA.

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CloneID	OBa238591
Clone ID Related Accession (Same CDS sequence)	XM_002700461.5
Accession Version	XM_002700461.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein Shroom2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002700461.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGAGCCCCG CGCGCGGCCG GAGCGACTGG CGGAGGCCGA GGCACGGACG 
GCCGATGGCG GGCGCCTGGT GGAGGTGCAG CTGAGCGGTG GCGCCCCTTG GGGCTTCACC 
TTGAAGGGCG GCCGCGAGCA CGGCGAGCCG CTGGTCATCA CCAAGGTTGA AGAAGGCAGC 
AAAGCCGCGG CCGTGGACAA ACTGCTGGCC GGAGACGAGA TCGTGGGCAT CAATGATGTC 
GGTCTGTCCG GGTTTAGACA GGAAGCCATC TGCCTGGTGA AGGGGTCACA TAAGACCCTG 
AAGCTGGTGG TGAAAAGGAG GAATGAACTG AACTGGAGGC CACACTCCTG GCATGCCACC 
AAGTTCTCTG ACAGCCAGTC TGAGCCAGCT GCACCTCTGC TTCCCTGCCC CTCTCGGCCG 
AGCCGGTACC ACGCCAGCTC CTCCTCCCAG GACCTGTCGG GCGGTGCCTG GGAGCCAGCG 
AACCTGCAGC GCACCTCGGA CCACTTCAGC TCGCTGGGGA GCGTCGACAG CCTGGACCCA 
TCCTCCCAGC CCGACCCGTC GGGGCGCCTC TCCGCGGCCA AGTCCAGCAG CAGCATCGAC 
CGCCTGGGCG GTGCCAGCAA GCGGGACTCA GCTTATGGTT CATTCTCCAC GCGCTCCAGC 
ACCCCGGACC ACACCCTGCC CAAGGCAGAC GCGTCCTCGG CCGAGAATAT CCTCTACAAA 
GTGGGCCTGT GGGAGGCCTC CGCTGCAGCG GACAGACGGC CGGGCCTGGC GGCTGGCGAC 
CCTCAGGGCA CCGAGGAGCC CCTGGGGTGC TTCCCACCCC GGGTCCCTGA GGACGGCCCC 
GAGCCCAAGC TAGCTGCTTG CGGGCGGTCC AGCTCCGGGC CCGTCTGGTA TGTTCCGGAT 
AAGAGAAGAG CCCCTCCGTC CCCTCCCCCG CCGCCTCCCC CGCTCCGCCG TGACAGCTTC 
GCTGCCACCA GGATCCACGA GCAGACCCAG GGCCCCACGG TCTCAGCAGA CGCAGCCCCA 
GCGCAGCCCT TGCCGGGCCT GAGCTGCGTG CAGCCCCACG GTGGCGACTG GAAGAGACCT 
GAACCCGCTG ATCAGCTGTT GAGGCCGGCG CGCGGGGCTG ACGGCCGGAG AGCTGCGAGC 
TCGGGGTGCG CTCCAGGGGC ACATGTGGAC TGTGGGTGGC CGGCCTGCGA CAGCGGGTCC 
CGGGCCCTCT TGGGGGCCCC CAGCCGGCTG CAGGCTTCTC TCTCGAGCAC TGACGTGCGC 
CGCCCGCAGC CCTCGCCCGT GTGCCAACAC CCGCGCCACT TGAGCGACGA GGGCCCCTTC 
TTCCGCAAGG GCCCCAGGGC CACCACGTGG CTAGGGGAGC CCCTCCCCGC TGACTGCCTC 
GGGGACAACA CCCCTGCTGC CATTGTCATC GGAGGGCCAC GCGCTGCAGA CCAGAGGGGC 
GACTGCAGCG CGCAGAGCCG CTACTACTGC GTGATGTCCA GACCCGGTCC CCAGTGGGGC 
GCCCCGGCCC CTCAGCCCCG CTGCTACCCG TCCCGGCTGG AGGGCGCCCC GGGCGCACGG 
CAGAGAAGCA GGGCAGACCC GGTGGACAGG GCACCCGAGG ACCCACCGGG GGCTGGCCTC 
CTGGACAGAG GTGGCAGGAA GAGGGTGGTG GGCAGCTTGG ACGACGGGGG CGGCGAGATC 
TGCCCCCTGC AGACGCCCAT GCTGCACTCG CTGAGCCGGG AAAGGGCGTG CCCGCCCGAG 
ACCCGCCACA AGGACATGGC GCCGGCGCCG CCTGAGGCCC TGGCAGGCAA GCCGCTCCGG 
AGGAGTGACC GCTTCGCCAC CACCCTGAGG AATGAGATCC AGCAGCGGCG AGCCAGGCTG 
CAGAAGAGCC GGAGCACGGC CACCTTGGCC AGCTCCGCGG AGGAGGAGGG GGAGGTGGAA 
GACGACGTGG AGACTGAGGC GCGCGGCTCC TGGGCGCAGC CAGTGGGCGC CCCGGAGACC 
ACCTTCCCCA GCGGCACCTA TAAGGACCAC TTGAAGGAGG CGCAGGCCCG CGTCCTGAGG 
GCCACGTCGT TCCGGCGCCG TGACCTGGAT CTCAGCCTGG CGGACCCGGA GCCAGCCCCC 
CGCGTCTGGG AGGGGGCCCC GGGTGCCAGG CCGCCCTTGT GCGCTGCCGG CAGTGGCAGT 
GGCAGCGGCA GCGCGCGCAT CGGGAGCCGG AGGCGCTTCA CTGCCGAGCA GAAACTGAAG 
TCCTACTCGG AGCCCGAGAA GATGAACGCG GTGGGGCTGA CGGGGGACCA GAGCCCTGCA 
CCCCATGATG AGACGCTGTG GACCTTTGCT GACAGGTGGA AGTTTTTTGA GGAAACCAGC 
AAGCCCGTGG GCCCCCAGCC CGGGCCGAGG CCGGCGCCCT GTGGCTTCCC CAGGGAGAAG 
CCGGAGATGC TGTGGACAGT GGGCCCTGGA AAAGACGGAA AGGCGGGGAG ACCCATCCCG 
CCAGAGCGGC TCGGCACCTT CGCAGAGTAT CAAGCCTCCT GGAGGGAACA GAGGAAAGTC 
CCGGAAGCCA GGGGTGGCGG GAGGTACCAC TCCACTGACA ACATCCTCGA CGTGGGTGTG 
GACCAGGAAG AGAGGCCACA GTACGTCCAC GAAAGGTCCC GGTCATCACC TTCCACGGAC 
CTCTACAAGC AGGACGCCTC CATGGAACCT CGATGGGAAG CAGGGGACCC GGAGGAGCAC 
ACGGAGCTTT CTTCGGCGGC ACCGGCCGAG GACAGAAGCC CCACCCCAAG ACAAGCAGAT 
GGTCAGCGTT CGGAGGACCA CCCAGGAGCA CTGCGGGACC CCCGGCAAAC CTCTGAACCC 
CCTGGCAGCA GGGAAGGGCC CGAGGTGTCC CTGGAGGCCC GCGGCCGGGC GGGCACTCTC 
CCTCGCGATT ACCGGTACCT GGAGCCGCGC ACGCCCCTGG AGCCACCCGC CCAGGGGCAG 
GAGCCCCGGC CCCTGAGCGC CGCCGCGACA GCCCGGAGAC CCGCCCTACA GAGGCCACAG 
CCGCCCAAGC GCGAGCTCTG GCCGCCGCCA GGGGACACGC CCAGCCGGCC TCACACCTCC 
GTGGCCTCCT CGGAGGCGGT GGACACGCGG AGCACGCGGC GCGTGGAGGA GCCCACGGCC 
CGGCCCTGGC GGGAGGCGCT GCTTCCCGCC CCGTTCCGGC CCCTGCAGAC CTCCGCCATG 
GACAGCTCGC GCTCGCCGTC GCCGCAGTTC GCGCCGCAGA AGCTGACGGA CAAGCCCCCT 
CTGCTCATCC AGGACGAGAA TGCTGCCAGA ATCGAGCGGG TGATGGACAA CAACACCACG 
GTGAAGATGG TGCCCATCAA GATAGTGCAT TCGGAGAGCC AGCCGGAGAA GGAGAGCCGC 
CAGGCACTGG CCCGCGTCCC CGAGCCACCT CCGCTGCCCC GGGGCCTCGA GCGGGACCAG 
ATCAAGACGC TCAGCACGTC TGAGCAGTCC TACTCACGCT TCTGCCCCTA CAGCCGCCAG 
GGTGCCGAGC CCCAGCCCGC CACCGGCGCC CTGGGGCCCC CCGCCAAGGA AGGCCGGGCC 
TCCCCTCCTG TGCTCAGCTA CGTGAAGGCC AAAGACAGGA CCGCCGACGA CCTCAAGTCA 
GAGGAGCTGG CCCGCGAGAT CGCGGGCAGG GATAAGTCGC TGGCGGACAT CCTAGACCCG 
GGCGTGAGGA TGAGAACCAC CATGGACCTC ATGGAGGGCA TCTTCCCCAA AGACGAGCAC 
CTCCTGGAAG GCGCACAGCA ACGGAGGAAG CTGCTGCCCA AGGGGCCCTC GCCCAGGACG 
GCGGAGGAGA GGAGGGAGGA GACCAGTGTG CTGGCAGCCG CACCCTTGGC CACCAACTCC 
GCCTACTACA GCACGTCAGC TCCCAAGGCG GAGCTGCTGA TAAAGATGAA GGACCTGCAG 
GAGCAGCAGG AGCCAGAGGA GGACTCAGGC AGTGACTTGG ACCACGACCT GTCAGTCAAA 
AAGCAGGAGC TCATTGAGAG CCTTGGCCGC AAGCTGCAGG TGCTGCGGGA GGCGCGGGAG 
AGCCTGCTGG AGGACGTGCA GGCCAACAGC GCGCTGGGCG ACGAGGTGGA GGCCCTGGTC 
AAGGCCGTGT GCAAGCCCAA TGAGTTTGAC AAGTTCCGCA TGTTCATCGG GGACCTCGAC 
AAGGTGGTGA ACCTCCTGCT GTCGCTGTCC GGCCGCCTGG CCCGCGTGGA GAACGCCCTC 
AACAACCTGG ACGACAGCAC GCCTCCGGGC GATCGGCAAT CCCTGCTCGA GAAGCAGCGC 
GTCCTGATCC AGCAGCACGA AGACGCCAGG GAGCTGAAGG AGAACCTGGA CCGCCGGGAA 
GGCATCGTCT TCGACATCCT GGTCAGCTAC CTGGGCCCCG AGAGCCTGGC GGACTACGAG 
CACTTCGTCA AGATGAAGTC GGCGCTCATC ATCGAACAGC GCGAGCTGGA AGACAAAATA 
CACCTGGGTG AGGAGCAGCT GAAGTGCCTC TTGGACAGCC TGCAGCCCGA GAGAGGCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700507.3
CDS	211..4713
Translation	MEGAEPRARPERLAEAEARTADGGRLVEVQLSGGAPWGFTLKGGREHGEPLVITKVEEGSKAAAVDKLLAGDEIVGINDVGLSGFRQEAICLVKGSHKTLKLVVKRRNELNWRPHSWHATKFSDSQSEPAAPLLPCPSRPSRYHASSSSQDLSGGAWEPANLQRTSDHFSSLGSVDSLDPSSQPDPSGRLSAAKSSSSIDRLGGASKRDSAYGSFSTRSSTPDHTLPKADASSAENILYKVGLWEASAAADRRPGLAAGDPQGTEEPLGCFPPRVPEDGPEPKLAACGRSSSGPVWYVPDKRRAPPSPPPPPPPLRRDSFAATRIHEQTQGPTVSADAAPAQPLPGLSCVQPHGGDWKRPEPADQLLRPARGADGRRAASSGCAPGAHVDCGWPACDSGSRALLGAPSRLQASLSSTDVRRPQPSPVCQHPRHLSDEGPFFRKGPRATTWLGEPLPADCLGDNTPAAIVIGGPRAADQRGDCSAQSRYYCVMSRPGPQWGAPAPQPRCYPSRLEGAPGARQRSRADPVDRAPEDPPGAGLLDRGGRKRVVGSLDDGGGEICPLQTPMLHSLSRERACPPETRHKDMAPAPPEALAGKPLRRSDRFATTLRNEIQQRRARLQKSRSTATLASSAEEEGEVEDDVETEARGSWAQPVGAPETTFPSGTYKDHLKEAQARVLRATSFRRRDLDLSLADPEPAPRVWEGAPGARPPLCAAGSGSGSGSARIGSRRRFTAEQKLKSYSEPEKMNAVGLTGDQSPAPHDETLWTFADRWKFFEETSKPVGPQPGPRPAPCGFPREKPEMLWTVGPGKDGKAGRPIPPERLGTFAEYQASWREQRKVPEARGGGRYHSTDNILDVGVDQEERPQYVHERSRSSPSTDLYKQDASMEPRWEAGDPEEHTELSSAAPAEDRSPTPRQADGQRSEDHPGALRDPRQTSEPPGSREGPEVSLEARGRAGTLPRDYRYLEPRTPLEPPAQGQEPRPLSAAATARRPALQRPQPPKRELWPPPGDTPSRPHTSVASSEAVDTRSTRRVEEPTARPWREALLPAPFRPLQTSAMDSSRSPSPQFAPQKLTDKPPLLIQDENAARIERVMDNNTTVKMVPIKIVHSESQPEKESRQALARVPEPPPLPRGLERDQIKTLSTSEQSYSRFCPYSRQGAEPQPATGALGPPAKEGRASPPVLSYVKAKDRTADDLKSEELAREIAGRDKSLADILDPGVRMRTTMDLMEGIFPKDEHLLEGAQQRRKLLPKGPSPRTAEERREETSVLAAAPLATNSAYYSTSAPKAELLIKMKDLQEQQEPEEDSGSDLDHDLSVKKQELIESLGRKLQVLREARESLLEDVQANSALGDEVEALVKAVCKPNEFDKFRMFIGDLDKVVNLLLSLSGRLARVENALNNLDDSTPPGDRQSLLEKQRVLIQQHEDARELKENLDRREGIVFDILVSYLGPESLADYEHFVKMKSALIIEQRELEDKIHLGEEQLKCLLDSLQPERGK

Target ORF information:

RefSeq Version	XM_002700461.5
Organism	Bos taurus(cattle)
Definition	Bos taurus shroom family member 2 (SHROOM2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700461.5

ORF Insert Sequence:

ATGGAGGGCG CCGAGCCCCG CGCGCGGCCG GAGCGACTGG CGGAGGCCGA GGCACGGACG 
GCCGATGGCG GGCGCCTGGT GGAGGTGCAG CTGAGCGGTG GCGCCCCTTG GGGCTTCACC 
TTGAAGGGCG GCCGCGAGCA CGGCGAGCCG CTGGTCATCA CCAAGGTTGA AGAAGGCAGC 
AAAGCCGCGG CCGTGGACAA ACTGCTGGCC GGAGACGAGA TCGTGGGCAT CAATGATGTC 
GGTCTGTCCG GGTTTAGACA GGAAGCCATC TGCCTGGTGA AGGGGTCACA TAAGACCCTG 
AAGCTGGTGG TGAAAAGGAG GAATGAACTG AACTGGAGGC CACACTCCTG GCATGCCACC 
AAGTTCTCTG ACAGCCAGTC TGAGCCAGCT GCACCTCTGC TTCCCTGCCC CTCTCGGCCG 
AGCCGGTACC ACGCCAGCTC CTCCTCCCAG GACCTGTCGG GCGGTGCCTG GGAGCCAGCG 
AACCTGCAGC GCACCTCGGA CCACTTCAGC TCGCTGGGGA GCGTCGACAG CCTGGACCCA 
TCCTCCCAGC CCGACCCGTC GGGGCGCCTC TCCGCGGCCA AGTCCAGCAG CAGCATCGAC 
CGCCTGGGCG GTGCCAGCAA GCGGGACTCA GCTTATGGTT CATTCTCCAC GCGCTCCAGC 
ACCCCGGACC ACACCCTGCC CAAGGCAGAC GCGTCCTCGG CCGAGAATAT CCTCTACAAA 
GTGGGCCTGT GGGAGGCCTC CGCTGCAGCG GACAGACGGC CGGGCCTGGC GGCTGGCGAC 
CCTCAGGGCA CCGAGGAGCC CCTGGGGTGC TTCCCACCCC GGGTCCCTGA GGACGGCCCC 
GAGCCCAAGC TAGCTGCTTG CGGGCGGTCC AGCTCCGGGC CCGTCTGGTA TGTTCCGGAT 
AAGAGAAGAG CCCCTCCGTC CCCTCCCCCG CCGCCTCCCC CGCTCCGCCG TGACAGCTTC 
GCTGCCACCA GGATCCACGA GCAGACCCAG GGCCCCACGG TCTCAGCAGA CGCAGCCCCA 
GCGCAGCCCT TGCCGGGCCT GAGCTGCGTG CAGCCCCACG GTGGCGACTG GAAGAGACCT 
GAACCCGCTG ATCAGCTGTT GAGGCCGGCG CGCGGGGCTG ACGGCCGGAG AGCTGCGAGC 
TCGGGGTGCG CTCCAGGGGC ACATGTGGAC TGTGGGTGGC CGGCCTGCGA CAGCGGGTCC 
CGGGCCCTCT TGGGGGCCCC CAGCCGGCTG CAGGCTTCTC TCTCGAGCAC TGACGTGCGC 
CGCCCGCAGC CCTCGCCCGT GTGCCAACAC CCGCGCCACT TGAGCGACGA GGGCCCCTTC 
TTCCGCAAGG GCCCCAGGGC CACCACGTGG CTAGGGGAGC CCCTCCCCGC TGACTGCCTC 
GGGGACAACA CCCCTGCTGC CATTGTCATC GGAGGGCCAC GCGCTGCAGA CCAGAGGGGC 
GACTGCAGCG CGCAGAGCCG CTACTACTGC GTGATGTCCA GACCCGGTCC CCAGTGGGGC 
GCCCCGGCCC CTCAGCCCCG CTGCTACCCG TCCCGGCTGG AGGGCGCCCC GGGCGCACGG 
CAGAGAAGCA GGGCAGACCC GGTGGACAGG GCACCCGAGG ACCCACCGGG GGCTGGCCTC 
CTGGACAGAG GTGGCAGGAA GAGGGTGGTG GGCAGCTTGG ACGACGGGGG CGGCGAGATC 
TGCCCCCTGC AGACGCCCAT GCTGCACTCG CTGAGCCGGG AAAGGGCGTG CCCGCCCGAG 
ACCCGCCACA AGGACATGGC GCCGGCGCCG CCTGAGGCCC TGGCAGGCAA GCCGCTCCGG 
AGGAGTGACC GCTTCGCCAC CACCCTGAGG AATGAGATCC AGCAGCGGCG AGCCAGGCTG 
CAGAAGAGCC GGAGCACGGC CACCTTGGCC AGCTCCGCGG AGGAGGAGGG GGAGGTGGAA 
GACGACGTGG AGACTGAGGC GCGCGGCTCC TGGGCGCAGC CAGTGGGCGC CCCGGAGACC 
ACCTTCCCCA GCGGCACCTA TAAGGACCAC TTGAAGGAGG CGCAGGCCCG CGTCCTGAGG 
GCCACGTCGT TCCGGCGCCG TGACCTGGAT CTCAGCCTGG CGGACCCGGA GCCAGCCCCC 
CGCGTCTGGG AGGGGGCCCC GGGTGCCAGG CCGCCCTTGT GCGCTGCCGG CAGTGGCAGT 
GGCAGCGGCA GCGCGCGCAT CGGGAGCCGG AGGCGCTTCA CTGCCGAGCA GAAACTGAAG 
TCCTACTCGG AGCCCGAGAA GATGAACGCG GTGGGGCTGA CGGGGGACCA GAGCCCTGCA 
CCCCATGATG AGACGCTGTG GACCTTTGCT GACAGGTGGA AGTTTTTTGA GGAAACCAGC 
AAGCCCGTGG GCCCCCAGCC CGGGCCGAGG CCGGCGCCCT GTGGCTTCCC CAGGGAGAAG 
CCGGAGATGC TGTGGACAGT GGGCCCTGGA AAAGACGGAA AGGCGGGGAG ACCCATCCCG 
CCAGAGCGGC TCGGCACCTT CGCAGAGTAT CAAGCCTCCT GGAGGGAACA GAGGAAAGTC 
CCGGAAGCCA GGGGTGGCGG GAGGTACCAC TCCACTGACA ACATCCTCGA CGTGGGTGTG 
GACCAGGAAG AGAGGCCACA GTACGTCCAC GAAAGGTCCC GGTCATCACC TTCCACGGAC 
CTCTACAAGC AGGACGCCTC CATGGAACCT CGATGGGAAG CAGGGGACCC GGAGGAGCAC 
ACGGAGCTTT CTTCGGCGGC ACCGGCCGAG GACAGAAGCC CCACCCCAAG ACAAGCAGAT 
GGTCAGCGTT CGGAGGACCA CCCAGGAGCA CTGCGGGACC CCCGGCAAAC CTCTGAACCC 
CCTGGCAGCA GGGAAGGGCC CGAGGTGTCC CTGGAGGCCC GCGGCCGGGC GGGCACTCTC 
CCTCGCGATT ACCGGTACCT GGAGCCGCGC ACGCCCCTGG AGCCACCCGC CCAGGGGCAG 
GAGCCCCGGC CCCTGAGCGC CGCCGCGACA GCCCGGAGAC CCGCCCTACA GAGGCCACAG 
CCGCCCAAGC GCGAGCTCTG GCCGCCGCCA GGGGACACGC CCAGCCGGCC TCACACCTCC 
GTGGCCTCCT CGGAGGCGGT GGACACGCGG AGCACGCGGC GCGTGGAGGA GCCCACGGCC 
CGGCCCTGGC GGGAGGCGCT GCTTCCCGCC CCGTTCCGGC CCCTGCAGAC CTCCGCCATG 
GACAGCTCGC GCTCGCCGTC GCCGCAGTTC GCGCCGCAGA AGCTGACGGA CAAGCCCCCT 
CTGCTCATCC AGGACGAGAA TGCTGCCAGA ATCGAGCGGG TGATGGACAA CAACACCACG 
GTGAAGATGG TGCCCATCAA GATAGTGCAT TCGGAGAGCC AGCCGGAGAA GGAGAGCCGC 
CAGGCACTGG CCCGCGTCCC CGAGCCACCT CCGCTGCCCC GGGGCCTCGA GCGGGACCAG 
ATCAAGACGC TCAGCACGTC TGAGCAGTCC TACTCACGCT TCTGCCCCTA CAGCCGCCAG 
GGTGCCGAGC CCCAGCCCGC CACCGGCGCC CTGGGGCCCC CCGCCAAGGA AGGCCGGGCC 
TCCCCTCCTG TGCTCAGCTA CGTGAAGGCC AAAGACAGGA CCGCCGACGA CCTCAAGTCA 
GAGGAGCTGG CCCGCGAGAT CGCGGGCAGG GATAAGTCGC TGGCGGACAT CCTAGACCCG 
GGCGTGAGGA TGAGAACCAC CATGGACCTC ATGGAGGGCA TCTTCCCCAA AGACGAGCAC 
CTCCTGGAAG GCGCACAGCA ACGGAGGAAG CTGCTGCCCA AGGGGCCCTC GCCCAGGACG 
GCGGAGGAGA GGAGGGAGGA GACCAGTGTG CTGGCAGCCG CACCCTTGGC CACCAACTCC 
GCCTACTACA GCACGTCAGC TCCCAAGGCG GAGCTGCTGA TAAAGATGAA GGACCTGCAG 
GAGCAGCAGG AGCCAGAGGA GGACTCAGGC AGTGACTTGG ACCACGACCT GTCAGTCAAA 
AAGCAGGAGC TCATTGAGAG CCTTGGCCGC AAGCTGCAGG TGCTGCGGGA GGCGCGGGAG 
AGCCTGCTGG AGGACGTGCA GGCCAACAGC GCGCTGGGCG ACGAGGTGGA GGCCCTGGTC 
AAGGCCGTGT GCAAGCCCAA TGAGTTTGAC AAGTTCCGCA TGTTCATCGG GGACCTCGAC 
AAGGTGGTGA ACCTCCTGCT GTCGCTGTCC GGCCGCCTGG CCCGCGTGGA GAACGCCCTC 
AACAACCTGG ACGACAGCAC GCCTCCGGGC GATCGGCAAT CCCTGCTCGA GAAGCAGCGC 
GTCCTGATCC AGCAGCACGA AGACGCCAGG GAGCTGAAGG AGAACCTGGA CCGCCGGGAA 
GGCATCGTCT TCGACATCCT GGTCAGCTAC CTGGGCCCCG AGAGCCTGGC GGACTACGAG 
CACTTCGTCA AGATGAAGTC GGCGCTCATC ATCGAACAGC GCGAGCTGGA AGACAAAATA 
CACCTGGGTG AGGAGCAGCT GAAGTGCCTC TTGGACAGCC TGCAGCCCGA GAGAGGCAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHROOM2 ( XM_002700461.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700507.2 Bos taurus shroom family member 2 (SHROOM2), mRNA.

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CloneID	OBa15217
Clone ID Related Accession (Same CDS sequence)	XM_002700461.4 , XM_601946.6
Accession Version	XM_002700461.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein Shroom2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104788.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002700461.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGAGCCCCG CGCGCGGCCG GAGCGACTGG CGGAGGCCGA GGCACGGACG 
GCCGATGGCG GGCGCCTGGT GGAGGTGCAG CTGAGCGGTG GCGCCCCTTG GGGCTTCACC 
TTGAAGGGCG GCCGCGAGCA CGGCGAGCCG CTGGTCATCA CCAAGGTTGA AGAAGGCAGC 
AAAGCCGCGG CCGTGGACAA ACTGCTGGCC GGAGACGAGA TCGTGGGCAT CAATGATGTC 
GGTCTGTCCG GGTTTAGACA GGAAGCCATC TGCCTGGTGA AGGGGTCACA TAAGACCCTG 
AAGCTGGTGG TGAAAAGGAG GAATGAACTG AACTGGAGGC CACACTCCTG GCATGCCACC 
AAGTTCTCTG ACAGCCAGTC TGAGCCAGCT GCACCTCTGC TTCCCTGCCC CTCTCGGCCG 
AGCCGGTACC ACGCCAGCTC CTCCTCCCAG GACCTGTCGG GCGGTGCCTG GGAGCCAGCG 
AACCTGCAGC GCACCTCGGA CCACTTCAGC TCGCTGGGGA GCGTCGACAG CCTGGACCCA 
TCCTCCCAGC CCGACCCGTC GGGGCGCCTC TCCGCGGCCA AGTCCAGCAG CAGCATCGAC 
CGCCTGGGCG GTGCCAGCAA GCGGGACTCA GCTTATGGTT CATTCTCCAC GCGCTCCAGC 
ACCCCGGACC ACACCCTGCC CAAGGCAGAC GCGTCCTCGG CCGAGAATAT CCTCTACAAA 
GTGGGCCTGT GGGAGGCCTC CGCTGCAGCG GACAGACGGC CGGGCCTGGC GGCTGGCGAC 
CCTCAGGGCA CCGAGGAGCC CCTGGGGTGC TTCCCACCCC GGGTCCCTGA GGACGGCCCC 
GAGCCCAAGC TAGCTGCTTG CGGGCGGTCC AGCTCCGGGC CCGTCTGGTA TGTTCCGGAT 
AAGAGAAGAG CCCCTCCGTC CCCTCCCCCG CCGCCTCCCC CGCTCCGCCG TGACAGCTTC 
GCTGCCACCA GGATCCACGA GCAGACCCAG GGCCCCACGG TCTCAGCAGA CGCAGCCCCA 
GCGCAGCCCT TGCCGGGCCT GAGCTGCGTG CAGCCCCACG GTGGCGACTG GAAGAGACCT 
GAACCCGCTG ATCAGCTGTT GAGGCCGGCG CGCGGGGCTG ACGGCCGGAG AGCTGCGAGC 
TCGGGGTGCG CTCCAGGGGC ACATGTGGAC TGTGGGTGGC CGGCCTGCGA CAGCGGGTCC 
CGGGCCCTCT TGGGGGCCCC CAGCCGGCTG CAGGCTTCTC TCTCGAGCAC TGACGTGCGC 
CGCCCGCAGC CCTCGCCCGT GTGCCAACAC CCGCGCCACT TGAGCGACGA GGGCCCCTTC 
TTCCGCAAGG GCCCCAGGGC CACCACGTGG CTAGGGGAGC CCCTCCCCGC TGACTGCCTC 
GGGGACAACA CCCCTGCTGC CATTGTCATC GGAGGGCCAC GCGCTGCAGA CCAGAGGGGC 
GACTGCAGCG CGCAGAGCCG CTACTACTGC GTGATGTCCA GACCCGGTCC CCAGTGGGGC 
GCCCCGGCCC CTCAGCCCCG CTGCTACCCG TCCCGGCTGG AGGGCGCCCC GGGCGCACGG 
CAGAGAAGCA GGGCAGACCC GGTGGACAGG GCACCCGAGG ACCCACCGGG GGCTGGCCTC 
CTGGACAGAG GTGGCAGGAA GAGGGTGGTG GGCAGCTTGG ACGACGGGGG CGGCGAGATC 
TGCCCCCTGC AGACGCCCAT GCTGCACTCG CTGAGCCGGG AAAGGGCGTG CCCGCCCGAG 
ACCCGCCACA AGGACATGGC GCCGGCGCCG CCTGAGGCCC TGGCAGGCAA GCCGCTCCGG 
AGGAGTGACC GCTTCGCCAC CACCCTGAGG AATGAGATCC AGCAGCGGCG AGCCAGGCTG 
CAGAAGAGCC GGAGCACGGC CACCTTGGCC AGCTCCGCGG AGGAGGAGGG GGAGGTGGAA 
GACGACGTGG AGACTGAGGC GCGCGGCTCC TGGGCGCAGC CAGTGGGCGC CCCGGAGACC 
ACCTTCCCCA GCGGCACCTA TAAGGACCAC TTGAAGGAGG CGCAGGCCCG CGTCCTGAGG 
GCCACGTCGT TCCGGCGCCG TGACCTGGAT CTCAGCCTGG CGGACCCGGA GCCAGCCCCC 
CGCGTCTGGG AGGGGGCCCC GGGTGCCAGG CCGCCCTTGT GCGCTGCCGG CAGTGGCAGT 
GGCAGCGGCA GCGCGCGCAT CGGGAGCCGG AGGCGCTTCA CTGCCGAGCA GAAACTGAAG 
TCCTACTCGG AGCCCGAGAA GATGAACGCG GTGGGGCTGA CGGGGGACCA GAGCCCTGCA 
CCCCATGATG AGACGCTGTG GACCTTTGCT GACAGGTGGA AGTTTTTTGA GGAAACCAGC 
AAGCCCGTGG GCCCCCAGCC CGGGCCGAGG CCGGCGCCCT GTGGCTTCCC CAGGGAGAAG 
CCGGAGATGC TGTGGACAGT GGGCCCTGGA AAAGACGGAA AGGCGGGGAG ACCCATCCCG 
CCAGAGCGGC TCGGCACCTT CGCAGAGTAT CAAGCCTCCT GGAGGGAACA GAGGAAAGTC 
CCGGAAGCCA GGGGTGGCGG GAGGTACCAC TCCACTGACA ACATCCTCGA CGTGGGTGTG 
GACCAGGAAG AGAGGCCACA GTACGTCCAC GAAAGGTCCC GGTCATCACC TTCCACGGAC 
CTCTACAAGC AGGACGCCTC CATGGAACCT CGATGGGAAG CAGGGGACCC GGAGGAGCAC 
ACGGAGCTTT CTTCGGCGGC ACCGGCCGAG GACAGAAGCC CCACCCCAAG ACAAGCAGAT 
GGTCAGCGTT CGGAGGACCA CCCAGGAGCA CTGCGGGACC CCCGGCAAAC CTCTGAACCC 
CCTGGCAGCA GGGAAGGGCC CGAGGTGTCC CTGGAGGCCC GCGGCCGGGC GGGCACTCTC 
CCTCGCGATT ACCGGTACCT GGAGCCGCGC ACGCCCCTGG AGCCACCCGC CCAGGGGCAG 
GAGCCCCGGC CCCTGAGCGC CGCCGCGACA GCCCGGAGAC CCGCCCTACA GAGGCCACAG 
CCGCCCAAGC GCGAGCTCTG GCCGCCGCCA GGGGACACGC CCAGCCGGCC TCACACCTCC 
GTGGCCTCCT CGGAGGCGGT GGACACGCGG AGCACGCGGC GCGTGGAGGA GCCCACGGCC 
CGGCCCTGGC GGGAGGCGCT GCTTCCCGCC CCGTTCCGGC CCCTGCAGAC CTCCGCCATG 
GACAGCTCGC GCTCGCCGTC GCCGCAGTTC GCGCCGCAGA AGCTGACGGA CAAGCCCCCT 
CTGCTCATCC AGGACGAGAA TGCTGCCAGA ATCGAGCGGG TGATGGACAA CAACACCACG 
GTGAAGATGG TGCCCATCAA GATAGTGCAT TCGGAGAGCC AGCCGGAGAA GGAGAGCCGC 
CAGGCACTGG CCCGCGTCCC CGAGCCACCT CCGCTGCCCC GGGGCCTCGA GCGGGACCAG 
ATCAAGACGC TCAGCACGTC TGAGCAGTCC TACTCACGCT TCTGCCCCTA CAGCCGCCAG 
GGTGCCGAGC CCCAGCCCGC CACCGGCGCC CTGGGGCCCC CCGCCAAGGA AGGCCGGGCC 
TCCCCTCCTG TGCTCAGCTA CGTGAAGGCC AAAGACAGGA CCGCCGACGA CCTCAAGTCA 
GAGGAGCTGG CCCGCGAGAT CGCGGGCAGG GATAAGTCGC TGGCGGACAT CCTAGACCCG 
GGCGTGAGGA TGAGAACCAC CATGGACCTC ATGGAGGGCA TCTTCCCCAA AGACGAGCAC 
CTCCTGGAAG GCGCACAGCA ACGGAGGAAG CTGCTGCCCA AGGGGCCCTC GCCCAGGACG 
GCGGAGGAGA GGAGGGAGGA GACCAGTGTG CTGGCAGCCG CACCCTTGGC CACCAACTCC 
GCCTACTACA GCACGTCAGC TCCCAAGGCG GAGCTGCTGA TAAAGATGAA GGACCTGCAG 
GAGCAGCAGG AGCCAGAGGA GGACTCAGGC AGTGACTTGG ACCACGACCT GTCAGTCAAA 
AAGGTAGGGA GGCCGAAATC CGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700507.2
CDS	218..4204
Translation	MEGAEPRARPERLAEAEARTADGGRLVEVQLSGGAPWGFTLKGGREHGEPLVITKVEEGSKAAAVDKLLAGDEIVGINDVGLSGFRQEAICLVKGSHKTLKLVVKRRNELNWRPHSWHATKFSDSQSEPAAPLLPCPSRPSRYHASSSSQDLSGGAWEPANLQRTSDHFSSLGSVDSLDPSSQPDPSGRLSAAKSSSSIDRLGGASKRDSAYGSFSTRSSTPDHTLPKADASSAENILYKVGLWEASAAADRRPGLAAGDPQGTEEPLGCFPPRVPEDGPEPKLAACGRSSSGPVWYVPDKRRAPPSPPPPPPPLRRDSFAATRIHEQTQGPTVSADAAPAQPLPGLSCVQPHGGDWKRPEPADQLLRPARGADGRRAASSGCAPGAHVDCGWPACDSGSRALLGAPSRLQASLSSTDVRRPQPSPVCQHPRHLSDEGPFFRKGPRATTWLGEPLPADCLGDNTPAAIVIGGPRAADQRGDCSAQSRYYCVMSRPGPQWGAPAPQPRCYPSRLEGAPGARQRSRADPVDRAPEDPPGAGLLDRGGRKRVVGSLDDGGGEICPLQTPMLHSLSRERACPPETRHKDMAPAPPEALAGKPLRRSDRFATTLRNEIQQRRARLQKSRSTATLASSAEEEGEVEDDVETEARGSWAQPVGAPETTFPSGTYKDHLKEAQARVLRATSFRRRDLDLSLADPEPAPRVWEGAPGARPPLCAAGSGSGSGSARIGSRRRFTAEQKLKSYSEPEKMNAVGLTGDQSPAPHDETLWTFADRWKFFEETSKPVGPQPGPRPAPCGFPREKPEMLWTVGPGKDGKAGRPIPPERLGTFAEYQASWREQRKVPEARGGGRYHSTDNILDVGVDQEERPQYVHERSRSSPSTDLYKQDASMEPRWEAGDPEEHTELSSAAPAEDRSPTPRQADGQRSEDHPGALRDPRQTSEPPGSREGPEVSLEARGRAGTLPRDYRYLEPRTPLEPPAQGQEPRPLSAAATARRPALQRPQPPKRELWPPPGDTPSRPHTSVASSEAVDTRSTRRVEEPTARPWREALLPAPFRPLQTSAMDSSRSPSPQFAPQKLTDKPPLLIQDENAARIERVMDNNTTVKMVPIKIVHSESQPEKESRQALARVPEPPPLPRGLERDQIKTLSTSEQSYSRFCPYSRQGAEPQPATGALGPPAKEGRASPPVLSYVKAKDRTADDLKSEELAREIAGRDKSLADILDPGVRMRTTMDLMEGIFPKDEHLLEGAQQRRKLLPKGPSPRTAEERREETSVLAAAPLATNSAYYSTSAPKAELLIKMKDLQEQQEPEEDSGSDLDHDLSVKKVGRPKSG

Target ORF information:

RefSeq Version	XM_002700461.4
Organism	Bos taurus(cattle)
Definition	Bos taurus shroom family member 2 (SHROOM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700461.4

ORF Insert Sequence:

ATGGAGGGCG CCGAGCCCCG CGCGCGGCCG GAGCGACTGG CGGAGGCCGA GGCACGGACG 
GCCGATGGCG GGCGCCTGGT GGAGGTGCAG CTGAGCGGTG GCGCCCCTTG GGGCTTCACC 
TTGAAGGGCG GCCGCGAGCA CGGCGAGCCG CTGGTCATCA CCAAGGTTGA AGAAGGCAGC 
AAAGCCGCGG CCGTGGACAA ACTGCTGGCC GGAGACGAGA TCGTGGGCAT CAATGATGTC 
GGTCTGTCCG GGTTTAGACA GGAAGCCATC TGCCTGGTGA AGGGGTCACA TAAGACCCTG 
AAGCTGGTGG TGAAAAGGAG GAATGAACTG AACTGGAGGC CACACTCCTG GCATGCCACC 
AAGTTCTCTG ACAGCCAGTC TGAGCCAGCT GCACCTCTGC TTCCCTGCCC CTCTCGGCCG 
AGCCGGTACC ACGCCAGCTC CTCCTCCCAG GACCTGTCGG GCGGTGCCTG GGAGCCAGCG 
AACCTGCAGC GCACCTCGGA CCACTTCAGC TCGCTGGGGA GCGTCGACAG CCTGGACCCA 
TCCTCCCAGC CCGACCCGTC GGGGCGCCTC TCCGCGGCCA AGTCCAGCAG CAGCATCGAC 
CGCCTGGGCG GTGCCAGCAA GCGGGACTCA GCTTATGGTT CATTCTCCAC GCGCTCCAGC 
ACCCCGGACC ACACCCTGCC CAAGGCAGAC GCGTCCTCGG CCGAGAATAT CCTCTACAAA 
GTGGGCCTGT GGGAGGCCTC CGCTGCAGCG GACAGACGGC CGGGCCTGGC GGCTGGCGAC 
CCTCAGGGCA CCGAGGAGCC CCTGGGGTGC TTCCCACCCC GGGTCCCTGA GGACGGCCCC 
GAGCCCAAGC TAGCTGCTTG CGGGCGGTCC AGCTCCGGGC CCGTCTGGTA TGTTCCGGAT 
AAGAGAAGAG CCCCTCCGTC CCCTCCCCCG CCGCCTCCCC CGCTCCGCCG TGACAGCTTC 
GCTGCCACCA GGATCCACGA GCAGACCCAG GGCCCCACGG TCTCAGCAGA CGCAGCCCCA 
GCGCAGCCCT TGCCGGGCCT GAGCTGCGTG CAGCCCCACG GTGGCGACTG GAAGAGACCT 
GAACCCGCTG ATCAGCTGTT GAGGCCGGCG CGCGGGGCTG ACGGCCGGAG AGCTGCGAGC 
TCGGGGTGCG CTCCAGGGGC ACATGTGGAC TGTGGGTGGC CGGCCTGCGA CAGCGGGTCC 
CGGGCCCTCT TGGGGGCCCC CAGCCGGCTG CAGGCTTCTC TCTCGAGCAC TGACGTGCGC 
CGCCCGCAGC CCTCGCCCGT GTGCCAACAC CCGCGCCACT TGAGCGACGA GGGCCCCTTC 
TTCCGCAAGG GCCCCAGGGC CACCACGTGG CTAGGGGAGC CCCTCCCCGC TGACTGCCTC 
GGGGACAACA CCCCTGCTGC CATTGTCATC GGAGGGCCAC GCGCTGCAGA CCAGAGGGGC 
GACTGCAGCG CGCAGAGCCG CTACTACTGC GTGATGTCCA GACCCGGTCC CCAGTGGGGC 
GCCCCGGCCC CTCAGCCCCG CTGCTACCCG TCCCGGCTGG AGGGCGCCCC GGGCGCACGG 
CAGAGAAGCA GGGCAGACCC GGTGGACAGG GCACCCGAGG ACCCACCGGG GGCTGGCCTC 
CTGGACAGAG GTGGCAGGAA GAGGGTGGTG GGCAGCTTGG ACGACGGGGG CGGCGAGATC 
TGCCCCCTGC AGACGCCCAT GCTGCACTCG CTGAGCCGGG AAAGGGCGTG CCCGCCCGAG 
ACCCGCCACA AGGACATGGC GCCGGCGCCG CCTGAGGCCC TGGCAGGCAA GCCGCTCCGG 
AGGAGTGACC GCTTCGCCAC CACCCTGAGG AATGAGATCC AGCAGCGGCG AGCCAGGCTG 
CAGAAGAGCC GGAGCACGGC CACCTTGGCC AGCTCCGCGG AGGAGGAGGG GGAGGTGGAA 
GACGACGTGG AGACTGAGGC GCGCGGCTCC TGGGCGCAGC CAGTGGGCGC CCCGGAGACC 
ACCTTCCCCA GCGGCACCTA TAAGGACCAC TTGAAGGAGG CGCAGGCCCG CGTCCTGAGG 
GCCACGTCGT TCCGGCGCCG TGACCTGGAT CTCAGCCTGG CGGACCCGGA GCCAGCCCCC 
CGCGTCTGGG AGGGGGCCCC GGGTGCCAGG CCGCCCTTGT GCGCTGCCGG CAGTGGCAGT 
GGCAGCGGCA GCGCGCGCAT CGGGAGCCGG AGGCGCTTCA CTGCCGAGCA GAAACTGAAG 
TCCTACTCGG AGCCCGAGAA GATGAACGCG GTGGGGCTGA CGGGGGACCA GAGCCCTGCA 
CCCCATGATG AGACGCTGTG GACCTTTGCT GACAGGTGGA AGTTTTTTGA GGAAACCAGC 
AAGCCCGTGG GCCCCCAGCC CGGGCCGAGG CCGGCGCCCT GTGGCTTCCC CAGGGAGAAG 
CCGGAGATGC TGTGGACAGT GGGCCCTGGA AAAGACGGAA AGGCGGGGAG ACCCATCCCG 
CCAGAGCGGC TCGGCACCTT CGCAGAGTAT CAAGCCTCCT GGAGGGAACA GAGGAAAGTC 
CCGGAAGCCA GGGGTGGCGG GAGGTACCAC TCCACTGACA ACATCCTCGA CGTGGGTGTG 
GACCAGGAAG AGAGGCCACA GTACGTCCAC GAAAGGTCCC GGTCATCACC TTCCACGGAC 
CTCTACAAGC AGGACGCCTC CATGGAACCT CGATGGGAAG CAGGGGACCC GGAGGAGCAC 
ACGGAGCTTT CTTCGGCGGC ACCGGCCGAG GACAGAAGCC CCACCCCAAG ACAAGCAGAT 
GGTCAGCGTT CGGAGGACCA CCCAGGAGCA CTGCGGGACC CCCGGCAAAC CTCTGAACCC 
CCTGGCAGCA GGGAAGGGCC CGAGGTGTCC CTGGAGGCCC GCGGCCGGGC GGGCACTCTC 
CCTCGCGATT ACCGGTACCT GGAGCCGCGC ACGCCCCTGG AGCCACCCGC CCAGGGGCAG 
GAGCCCCGGC CCCTGAGCGC CGCCGCGACA GCCCGGAGAC CCGCCCTACA GAGGCCACAG 
CCGCCCAAGC GCGAGCTCTG GCCGCCGCCA GGGGACACGC CCAGCCGGCC TCACACCTCC 
GTGGCCTCCT CGGAGGCGGT GGACACGCGG AGCACGCGGC GCGTGGAGGA GCCCACGGCC 
CGGCCCTGGC GGGAGGCGCT GCTTCCCGCC CCGTTCCGGC CCCTGCAGAC CTCCGCCATG 
GACAGCTCGC GCTCGCCGTC GCCGCAGTTC GCGCCGCAGA AGCTGACGGA CAAGCCCCCT 
CTGCTCATCC AGGACGAGAA TGCTGCCAGA ATCGAGCGGG TGATGGACAA CAACACCACG 
GTGAAGATGG TGCCCATCAA GATAGTGCAT TCGGAGAGCC AGCCGGAGAA GGAGAGCCGC 
CAGGCACTGG CCCGCGTCCC CGAGCCACCT CCGCTGCCCC GGGGCCTCGA GCGGGACCAG 
ATCAAGACGC TCAGCACGTC TGAGCAGTCC TACTCACGCT TCTGCCCCTA CAGCCGCCAG 
GGTGCCGAGC CCCAGCCCGC CACCGGCGCC CTGGGGCCCC CCGCCAAGGA AGGCCGGGCC 
TCCCCTCCTG TGCTCAGCTA CGTGAAGGCC AAAGACAGGA CCGCCGACGA CCTCAAGTCA 
GAGGAGCTGG CCCGCGAGAT CGCGGGCAGG GATAAGTCGC TGGCGGACAT CCTAGACCCG 
GGCGTGAGGA TGAGAACCAC CATGGACCTC ATGGAGGGCA TCTTCCCCAA AGACGAGCAC 
CTCCTGGAAG GCGCACAGCA ACGGAGGAAG CTGCTGCCCA AGGGGCCCTC GCCCAGGACG 
GCGGAGGAGA GGAGGGAGGA GACCAGTGTG CTGGCAGCCG CACCCTTGGC CACCAACTCC 
GCCTACTACA GCACGTCAGC TCCCAAGGCG GAGCTGCTGA TAAAGATGAA GGACCTGCAG 
GAGCAGCAGG AGCCAGAGGA GGACTCAGGC AGTGACTTGG ACCACGACCT GTCAGTCAAA 
AAGGTAGGGA GGCCGAAATC CGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHROOM2 ( XM_601946.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_601946.4 Bos taurus shroom family member 2 (SHROOM2), mRNA.

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CloneID	OBa15217
Clone ID Related Accession (Same CDS sequence)	XM_002700461.4 , XM_601946.6
Accession Version	XM_601946.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein Shroom2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_601946.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGAGCCCCG CGCGCGGCCG GAGCGACTGG CGGAGGCCGA GGCACGGACG 
GCCGATGGCG GGCGCCTGGT GGAGGTGCAG CTGAGCGGTG GCGCCCCTTG GGGCTTCACC 
TTGAAGGGCG GCCGCGAGCA CGGCGAGCCG CTGGTCATCA CCAAGGTTGA AGAAGGCAGC 
AAAGCCGCGG CCGTGGACAA ACTGCTGGCC GGAGACGAGA TCGTGGGCAT CAATGATGTC 
GGTCTGTCCG GGTTTAGACA GGAAGCCATC TGCCTGGTGA AGGGGTCACA TAAGACCCTG 
AAGCTGGTGG TGAAAAGGAG GAATGAACTG AACTGGAGGC CACACTCCTG GCATGCCACC 
AAGTTCTCTG ACAGCCAGTC TGAGCCAGCT GCACCTCTGC TTCCCTGCCC CTCTCGGCCG 
AGCCGGTACC ACGCCAGCTC CTCCTCCCAG GACCTGTCGG GCGGTGCCTG GGAGCCAGCG 
AACCTGCAGC GCACCTCGGA CCACTTCAGC TCGCTGGGGA GCGTCGACAG CCTGGACCCA 
TCCTCCCAGC CCGACCCGTC GGGGCGCCTC TCCGCGGCCA AGTCCAGCAG CAGCATCGAC 
CGCCTGGGCG GTGCCAGCAA GCGGGACTCA GCTTATGGTT CATTCTCCAC GCGCTCCAGC 
ACCCCGGACC ACACCCTGCC CAAGGCAGAC GCGTCCTCGG CCGAGAATAT CCTCTACAAA 
GTGGGCCTGT GGGAGGCCTC CGCTGCAGCG GACAGACGGC CGGGCCTGGC GGCTGGCGAC 
CCTCAGGGCA CCGAGGAGCC CCTGGGGTGC TTCCCACCCC GGGTCCCTGA GGACGGCCCC 
GAGCCCAAGC TAGCTGCTTG CGGGCGGTCC AGCTCCGGGC CCGTCTGGTA TGTTCCGGAT 
AAGAGAAGAG CCCCTCCGTC CCCTCCCCCG CCGCCTCCCC CGCTCCGCCG TGACAGCTTC 
GCTGCCACCA GGATCCACGA GCAGACCCAG GGCCCCACGG TCTCAGCAGA CGCAGCCCCA 
GCGCAGCCCT TGCCGGGCCT GAGCTGCGTG CAGCCCCACG GTGGCGACTG GAAGAGACCT 
GAACCCGCTG ATCAGCTGTT GAGGCCGGCG CGCGGGGCTG ACGGCCGGAG AGCTGCGAGC 
TCGGGGTGCG CTCCAGGGGC ACATGTGGAC TGTGGGTGGC CGGCCTGCGA CAGCGGGTCC 
CGGGCCCTCT TGGGGGCCCC CAGCCGGCTG CAGGCTTCTC TCTCGAGCAC TGACGTGCGC 
CGCCCGCAGC CCTCGCCCGT GTGCCAACAC CCGCGCCACT TGAGCGACGA GGGCCCCTTC 
TTCCGCAAGG GCCCCAGGGC CACCACGTGG CTAGGGGAGC CCCTCCCCGC TGACTGCCTC 
GGGGACAACA CCCCTGCTGC CATTGTCATC GGAGGGCCAC GCGCTGCAGA CCAGAGGGGC 
GACTGCAGCG CGCAGAGCCG CTACTACTGC GTGATGTCCA GACCCGGTCC CCAGTGGGGC 
GCCCCGGCCC CTCAGCCCCG CTGCTACCCG TCCCGGCTGG AGGGCGCCCC GGGCGCACGG 
CAGAGAAGCA GGGCAGACCC GGTGGACAGG GCACCCGAGG ACCCACCGGG GGCTGGCCTC 
CTGGACAGAG GTGGCAGGAA GAGGGTGGTG GGCAGCTTGG ACGACGGGGG CGGCGAGATC 
TGCCCCCTGC AGACGCCCAT GCTGCACTCG CTGAGCCGGG AAAGGGCGTG CCCGCCCGAG 
ACCCGCCACA AGGACATGGC GCCGGCGCCG CCTGAGGCCC TGGCAGGCAA GCCGCTCCGG 
AGGAGTGACC GCTTCGCCAC CACCCTGAGG AATGAGATCC AGCAGCGGCG AGCCAGGCTG 
CAGAAGAGCC GGAGCACGGC CACCTTGGCC AGCTCCGCGG AGGAGGAGGG GGAGGTGGAA 
GACGACGTGG AGACTGAGGC GCGCGGCTCC TGGGCGCAGC CAGTGGGCGC CCCGGAGACC 
ACCTTCCCCA GCGGCACCTA TAAGGACCAC TTGAAGGAGG CGCAGGCCCG CGTCCTGAGG 
GCCACGTCGT TCCGGCGCCG TGACCTGGAT CTCAGCCTGG CGGACCCGGA GCCAGCCCCC 
CGCGTCTGGG AGGGGGCCCC GGGTGCCAGG CCGCCCTTGT GCGCTGCCGG CAGTGGCAGT 
GGCAGCGGCA GCGCGCGCAT CGGGAGCCGG AGGCGCTTCA CTGCCGAGCA GAAACTGAAG 
TCCTACTCGG AGCCCGAGAA GATGAACGCG GTGGGGCTGA CGGGGGACCA GAGCCCTGCA 
CCCCATGATG AGACGCTGTG GACCTTTGCT GACAGGTGGA AGTTTTTTGA GGAAACCAGC 
AAGCCCGTGG GCCCCCAGCC CGGGCCGAGG CCGGCGCCCT GTGGCTTCCC CAGGGAGAAG 
CCGGAGATGC TGTGGACAGT GGGCCCTGGA AAAGACGGAA AGGCGGGGAG ACCCATCCCG 
CCAGAGCGGC TCGGCACCTT CGCAGAGTAT CAAGCCTCCT GGAGGGAACA GAGGAAAGTC 
CCGGAAGCCA GGGGTGGCGG GAGGTACCAC TCCACTGACA ACATCCTCGA CGTGGGTGTG 
GACCAGGAAG AGAGGCCACA GTACGTCCAC GAAAGGTCCC GGTCATCACC TTCCACGGAC 
CTCTACAAGC AGGACGCCTC CATGGAACCT CGATGGGAAG CAGGGGACCC GGAGGAGCAC 
ACGGAGCTTT CTTCGGCGGC ACCGGCCGAG GACAGAAGCC CCACCCCAAG ACAAGCAGAT 
GGTCAGCGTT CGGAGGACCA CCCAGGAGCA CTGCGGGACC CCCGGCAAAC CTCTGAACCC 
CCTGGCAGCA GGGAAGGGCC CGAGGTGTCC CTGGAGGCCC GCGGCCGGGC GGGCACTCTC 
CCTCGCGATT ACCGGTACCT GGAGCCGCGC ACGCCCCTGG AGCCACCCGC CCAGGGGCAG 
GAGCCCCGGC CCCTGAGCGC CGCCGCGACA GCCCGGAGAC CCGCCCTACA GAGGCCACAG 
CCGCCCAAGC GCGAGCTCTG GCCGCCGCCA GGGGACACGC CCAGCCGGCC TCACACCTCC 
GTGGCCTCCT CGGAGGCGGT GGACACGCGG AGCACGCGGC GCGTGGAGGA GCCCACGGCC 
CGGCCCTGGC GGGAGGCGCT GCTTCCCGCC CCGTTCCGGC CCCTGCAGAC CTCCGCCATG 
GACAGCTCGC GCTCGCCGTC GCCGCAGTTC GCGCCGCAGA AGCTGACGGA CAAGCCCCCT 
CTGCTCATCC AGGACGAGAA TGCTGCCAGA ATCGAGCGGG TGATGGACAA CAACACCACG 
GTGAAGATGG TGCCCATCAA GATAGTGCAT TCGGAGAGCC AGCCGGAGAA GGAGAGCCGC 
CAGGCACTGG CCCGCGTCCC CGAGCCACCT CCGCTGCCCC GGGGCCTCGA GCGGGACCAG 
ATCAAGACGC TCAGCACGTC TGAGCAGTCC TACTCACGCT TCTGCCCCTA CAGCCGCCAG 
GGTGCCGAGC CCCAGCCCGC CACCGGCGCC CTGGGGCCCC CCGCCAAGGA AGGCCGGGCC 
TCCCCTCCTG TGCTCAGCTA CGTGAAGGCC AAAGACAGGA CCGCCGACGA CCTCAAGTCA 
GAGGAGCTGG CCCGCGAGAT CGCGGGCAGG GATAAGTCGC TGGCGGACAT CCTAGACCCG 
GGCGTGAGGA TGAGAACCAC CATGGACCTC ATGGAGGGCA TCTTCCCCAA AGACGAGCAC 
CTCCTGGAAG GCGCACAGCA ACGGAGGAAG CTGCTGCCCA AGGGGCCCTC GCCCAGGACG 
GCGGAGGAGA GGAGGGAGGA GACCAGTGTG CTGGCAGCCG CACCCTTGGC CACCAACTCC 
GCCTACTACA GCACGTCAGC TCCCAAGGCG GAGCTGCTGA TAAAGATGAA GGACCTGCAG 
GAGCAGCAGG AGCCAGAGGA GGACTCAGGC AGTGACTTGG ACCACGACCT GTCAGTCAAA 
AAGGTAGGGA GGCCGAAATC CGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_601946.4
CDS	218..4204
Translation	MEGAEPRARPERLAEAEARTADGGRLVEVQLSGGAPWGFTLKGGREHGEPLVITKVEEGSKAAAVDKLLAGDEIVGINDVGLSGFRQEAICLVKGSHKTLKLVVKRRNELNWRPHSWHATKFSDSQSEPAAPLLPCPSRPSRYHASSSSQDLSGGAWEPANLQRTSDHFSSLGSVDSLDPSSQPDPSGRLSAAKSSSSIDRLGGASKRDSAYGSFSTRSSTPDHTLPKADASSAENILYKVGLWEASAAADRRPGLAAGDPQGTEEPLGCFPPRVPEDGPEPKLAACGRSSSGPVWYVPDKRRAPPSPPPPPPPLRRDSFAATRIHEQTQGPTVSADAAPAQPLPGLSCVQPHGGDWKRPEPADQLLRPARGADGRRAASSGCAPGAHVDCGWPACDSGSRALLGAPSRLQASLSSTDVRRPQPSPVCQHPRHLSDEGPFFRKGPRATTWLGEPLPADCLGDNTPAAIVIGGPRAADQRGDCSAQSRYYCVMSRPGPQWGAPAPQPRCYPSRLEGAPGARQRSRADPVDRAPEDPPGAGLLDRGGRKRVVGSLDDGGGEICPLQTPMLHSLSRERACPPETRHKDMAPAPPEALAGKPLRRSDRFATTLRNEIQQRRARLQKSRSTATLASSAEEEGEVEDDVETEARGSWAQPVGAPETTFPSGTYKDHLKEAQARVLRATSFRRRDLDLSLADPEPAPRVWEGAPGARPPLCAAGSGSGSGSARIGSRRRFTAEQKLKSYSEPEKMNAVGLTGDQSPAPHDETLWTFADRWKFFEETSKPVGPQPGPRPAPCGFPREKPEMLWTVGPGKDGKAGRPIPPERLGTFAEYQASWREQRKVPEARGGGRYHSTDNILDVGVDQEERPQYVHERSRSSPSTDLYKQDASMEPRWEAGDPEEHTELSSAAPAEDRSPTPRQADGQRSEDHPGALRDPRQTSEPPGSREGPEVSLEARGRAGTLPRDYRYLEPRTPLEPPAQGQEPRPLSAAATARRPALQRPQPPKRELWPPPGDTPSRPHTSVASSEAVDTRSTRRVEEPTARPWREALLPAPFRPLQTSAMDSSRSPSPQFAPQKLTDKPPLLIQDENAARIERVMDNNTTVKMVPIKIVHSESQPEKESRQALARVPEPPPLPRGLERDQIKTLSTSEQSYSRFCPYSRQGAEPQPATGALGPPAKEGRASPPVLSYVKAKDRTADDLKSEELAREIAGRDKSLADILDPGVRMRTTMDLMEGIFPKDEHLLEGAQQRRKLLPKGPSPRTAEERREETSVLAAAPLATNSAYYSTSAPKAELLIKMKDLQEQQEPEEDSGSDLDHDLSVKKVGRPKSG

Target ORF information:

RefSeq Version	XM_601946.6
Organism	Bos taurus(cattle)
Definition	Bos taurus shroom family member 2 (SHROOM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_601946.6

ORF Insert Sequence:

ATGGAGGGCG CCGAGCCCCG CGCGCGGCCG GAGCGACTGG CGGAGGCCGA GGCACGGACG 
GCCGATGGCG GGCGCCTGGT GGAGGTGCAG CTGAGCGGTG GCGCCCCTTG GGGCTTCACC 
TTGAAGGGCG GCCGCGAGCA CGGCGAGCCG CTGGTCATCA CCAAGGTTGA AGAAGGCAGC 
AAAGCCGCGG CCGTGGACAA ACTGCTGGCC GGAGACGAGA TCGTGGGCAT CAATGATGTC 
GGTCTGTCCG GGTTTAGACA GGAAGCCATC TGCCTGGTGA AGGGGTCACA TAAGACCCTG 
AAGCTGGTGG TGAAAAGGAG GAATGAACTG AACTGGAGGC CACACTCCTG GCATGCCACC 
AAGTTCTCTG ACAGCCAGTC TGAGCCAGCT GCACCTCTGC TTCCCTGCCC CTCTCGGCCG 
AGCCGGTACC ACGCCAGCTC CTCCTCCCAG GACCTGTCGG GCGGTGCCTG GGAGCCAGCG 
AACCTGCAGC GCACCTCGGA CCACTTCAGC TCGCTGGGGA GCGTCGACAG CCTGGACCCA 
TCCTCCCAGC CCGACCCGTC GGGGCGCCTC TCCGCGGCCA AGTCCAGCAG CAGCATCGAC 
CGCCTGGGCG GTGCCAGCAA GCGGGACTCA GCTTATGGTT CATTCTCCAC GCGCTCCAGC 
ACCCCGGACC ACACCCTGCC CAAGGCAGAC GCGTCCTCGG CCGAGAATAT CCTCTACAAA 
GTGGGCCTGT GGGAGGCCTC CGCTGCAGCG GACAGACGGC CGGGCCTGGC GGCTGGCGAC 
CCTCAGGGCA CCGAGGAGCC CCTGGGGTGC TTCCCACCCC GGGTCCCTGA GGACGGCCCC 
GAGCCCAAGC TAGCTGCTTG CGGGCGGTCC AGCTCCGGGC CCGTCTGGTA TGTTCCGGAT 
AAGAGAAGAG CCCCTCCGTC CCCTCCCCCG CCGCCTCCCC CGCTCCGCCG TGACAGCTTC 
GCTGCCACCA GGATCCACGA GCAGACCCAG GGCCCCACGG TCTCAGCAGA CGCAGCCCCA 
GCGCAGCCCT TGCCGGGCCT GAGCTGCGTG CAGCCCCACG GTGGCGACTG GAAGAGACCT 
GAACCCGCTG ATCAGCTGTT GAGGCCGGCG CGCGGGGCTG ACGGCCGGAG AGCTGCGAGC 
TCGGGGTGCG CTCCAGGGGC ACATGTGGAC TGTGGGTGGC CGGCCTGCGA CAGCGGGTCC 
CGGGCCCTCT TGGGGGCCCC CAGCCGGCTG CAGGCTTCTC TCTCGAGCAC TGACGTGCGC 
CGCCCGCAGC CCTCGCCCGT GTGCCAACAC CCGCGCCACT TGAGCGACGA GGGCCCCTTC 
TTCCGCAAGG GCCCCAGGGC CACCACGTGG CTAGGGGAGC CCCTCCCCGC TGACTGCCTC 
GGGGACAACA CCCCTGCTGC CATTGTCATC GGAGGGCCAC GCGCTGCAGA CCAGAGGGGC 
GACTGCAGCG CGCAGAGCCG CTACTACTGC GTGATGTCCA GACCCGGTCC CCAGTGGGGC 
GCCCCGGCCC CTCAGCCCCG CTGCTACCCG TCCCGGCTGG AGGGCGCCCC GGGCGCACGG 
CAGAGAAGCA GGGCAGACCC GGTGGACAGG GCACCCGAGG ACCCACCGGG GGCTGGCCTC 
CTGGACAGAG GTGGCAGGAA GAGGGTGGTG GGCAGCTTGG ACGACGGGGG CGGCGAGATC 
TGCCCCCTGC AGACGCCCAT GCTGCACTCG CTGAGCCGGG AAAGGGCGTG CCCGCCCGAG 
ACCCGCCACA AGGACATGGC GCCGGCGCCG CCTGAGGCCC TGGCAGGCAA GCCGCTCCGG 
AGGAGTGACC GCTTCGCCAC CACCCTGAGG AATGAGATCC AGCAGCGGCG AGCCAGGCTG 
CAGAAGAGCC GGAGCACGGC CACCTTGGCC AGCTCCGCGG AGGAGGAGGG GGAGGTGGAA 
GACGACGTGG AGACTGAGGC GCGCGGCTCC TGGGCGCAGC CAGTGGGCGC CCCGGAGACC 
ACCTTCCCCA GCGGCACCTA TAAGGACCAC TTGAAGGAGG CGCAGGCCCG CGTCCTGAGG 
GCCACGTCGT TCCGGCGCCG TGACCTGGAT CTCAGCCTGG CGGACCCGGA GCCAGCCCCC 
CGCGTCTGGG AGGGGGCCCC GGGTGCCAGG CCGCCCTTGT GCGCTGCCGG CAGTGGCAGT 
GGCAGCGGCA GCGCGCGCAT CGGGAGCCGG AGGCGCTTCA CTGCCGAGCA GAAACTGAAG 
TCCTACTCGG AGCCCGAGAA GATGAACGCG GTGGGGCTGA CGGGGGACCA GAGCCCTGCA 
CCCCATGATG AGACGCTGTG GACCTTTGCT GACAGGTGGA AGTTTTTTGA GGAAACCAGC 
AAGCCCGTGG GCCCCCAGCC CGGGCCGAGG CCGGCGCCCT GTGGCTTCCC CAGGGAGAAG 
CCGGAGATGC TGTGGACAGT GGGCCCTGGA AAAGACGGAA AGGCGGGGAG ACCCATCCCG 
CCAGAGCGGC TCGGCACCTT CGCAGAGTAT CAAGCCTCCT GGAGGGAACA GAGGAAAGTC 
CCGGAAGCCA GGGGTGGCGG GAGGTACCAC TCCACTGACA ACATCCTCGA CGTGGGTGTG 
GACCAGGAAG AGAGGCCACA GTACGTCCAC GAAAGGTCCC GGTCATCACC TTCCACGGAC 
CTCTACAAGC AGGACGCCTC CATGGAACCT CGATGGGAAG CAGGGGACCC GGAGGAGCAC 
ACGGAGCTTT CTTCGGCGGC ACCGGCCGAG GACAGAAGCC CCACCCCAAG ACAAGCAGAT 
GGTCAGCGTT CGGAGGACCA CCCAGGAGCA CTGCGGGACC CCCGGCAAAC CTCTGAACCC 
CCTGGCAGCA GGGAAGGGCC CGAGGTGTCC CTGGAGGCCC GCGGCCGGGC GGGCACTCTC 
CCTCGCGATT ACCGGTACCT GGAGCCGCGC ACGCCCCTGG AGCCACCCGC CCAGGGGCAG 
GAGCCCCGGC CCCTGAGCGC CGCCGCGACA GCCCGGAGAC CCGCCCTACA GAGGCCACAG 
CCGCCCAAGC GCGAGCTCTG GCCGCCGCCA GGGGACACGC CCAGCCGGCC TCACACCTCC 
GTGGCCTCCT CGGAGGCGGT GGACACGCGG AGCACGCGGC GCGTGGAGGA GCCCACGGCC 
CGGCCCTGGC GGGAGGCGCT GCTTCCCGCC CCGTTCCGGC CCCTGCAGAC CTCCGCCATG 
GACAGCTCGC GCTCGCCGTC GCCGCAGTTC GCGCCGCAGA AGCTGACGGA CAAGCCCCCT 
CTGCTCATCC AGGACGAGAA TGCTGCCAGA ATCGAGCGGG TGATGGACAA CAACACCACG 
GTGAAGATGG TGCCCATCAA GATAGTGCAT TCGGAGAGCC AGCCGGAGAA GGAGAGCCGC 
CAGGCACTGG CCCGCGTCCC CGAGCCACCT CCGCTGCCCC GGGGCCTCGA GCGGGACCAG 
ATCAAGACGC TCAGCACGTC TGAGCAGTCC TACTCACGCT TCTGCCCCTA CAGCCGCCAG 
GGTGCCGAGC CCCAGCCCGC CACCGGCGCC CTGGGGCCCC CCGCCAAGGA AGGCCGGGCC 
TCCCCTCCTG TGCTCAGCTA CGTGAAGGCC AAAGACAGGA CCGCCGACGA CCTCAAGTCA 
GAGGAGCTGG CCCGCGAGAT CGCGGGCAGG GATAAGTCGC TGGCGGACAT CCTAGACCCG 
GGCGTGAGGA TGAGAACCAC CATGGACCTC ATGGAGGGCA TCTTCCCCAA AGACGAGCAC 
CTCCTGGAAG GCGCACAGCA ACGGAGGAAG CTGCTGCCCA AGGGGCCCTC GCCCAGGACG 
GCGGAGGAGA GGAGGGAGGA GACCAGTGTG CTGGCAGCCG CACCCTTGGC CACCAACTCC 
GCCTACTACA GCACGTCAGC TCCCAAGGCG GAGCTGCTGA TAAAGATGAA GGACCTGCAG 
GAGCAGCAGG AGCCAGAGGA GGACTCAGGC AGTGACTTGG ACCACGACCT GTCAGTCAAA 
AAGGTAGGGA GGCCGAAATC CGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SHROOM2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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