NEK10 cDNA ORF clone, Bos taurus(cattle)

The following NEK10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa229231 XM_010817544.3
Latest version!
Bos taurus NIMA related kinase 10 (NEK10), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa50538 XM_010817544.2
Latest version!
Bos taurus NIMA related kinase 10 (NEK10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa50538 XM_015468659.1
Latest version!
Bos taurus NIMA related kinase 10 (NEK10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa50539 XM_015468660.1
Latest version!
Bos taurus NIMA related kinase 10 (NEK10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa50539 XM_010817548.2
Latest version!
Bos taurus NIMA related kinase 10 (NEK10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa50540 XM_002707776.5
Latest version!
Bos taurus NIMA related kinase 10 (NEK10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa50540 XM_015468661.1
Latest version!
Bos taurus NIMA related kinase 10 (NEK10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa229231
    Clone ID Related Accession (Same CDS sequence) XM_010817544.3
    Accession Version XM_010817544.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: serine/threonine-protein kinase Nek10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817544.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGACAAAAA AGTGAAGGCC ACTGAAAAAT CAACTGATAA AAAACAGCAA 
    GGAGTCAGCA TCAGGGACTA TTCAGATCTT AAAAGACTTC TGTGCCTTTT GAACGTTCAA
    TCAAGCAAGC AACAGCTTCC AGCCATTAAC TTCGATAGCG TCCAGAACAG CATAATGAAG
    TCTGAGCCTG CCGTCAGGAC AGGTGGGCAC AGAGCTCGAG GTCGGTGGCA TGAGTCCACA
    GAAGCTGTTG AACTCGAAAA TTTTAGTATA AACTACAAGA ATGAGAGAAA TTTCAGCAAA
    CATCCCCAGC ATAAACTATT TCAGGAGATA TTTACAGCTT TGGTGAAAAA TAGACTCATA
    TGCAGAGAGT GGGTTGATCG CGCTCCATCT CTTCATTTTC TGAGAGTGTT AATTTGTCTG
    AGACTCCTCA TGAGGGATCC ATGTTATCAG GAAATACTTC ATAGCTTGGG TGGAATTGAA
    AACCTAGCTC AGTACATGGA GATCGTGGCG CACGGGTACC TCGGCTACGG GGAGGCGCAG
    CACAGCGTGG ACAAGCTGGT GAACATGACG TATATTTTTC AAAAACTTGC TGCTGTCAGA
    GACCAAAGAG AATGGGTCAC CACAAGCGGA GCTCACAAGA CATTAGTGAA TTTACTCAGT
    GCCCGAGATA CGAATGTTCT GCTGGGTGCT CTTCTGGCTC TGGCTAGTTT AGCTGAAAGT
    CCAGAGTGTA GGGAGAAGAT AAGTGAGCTC AACATTGTAG AAAATCTGCT GATGATTTTA
    CACGAATATG ACTTGCTTTC CAAAAGGCTG ACAGCCGAGC TGCTGCGCCT GCTCTGTGCC
    GAGCGCCAGG TGAGAGAGCA GGTGAAGCTC TATGAGGGGG TCCCCGTCCT GCTCAGCCTG
    CTGCACTCAG ACCACCTGAA GCTGCTATGG AGTGTCGTCT GGATTCTGGT GCAGATTTGC
    GAGGACCCAG AGACCAGCGT GGAAATCCGC ATCTGGGGCG GCATCAAGCA ACTTCTTCAT
    ATTTTGCGAG GAGACAGAAA TTTTGTTTCT GATCGTTCCT CCATTGGAAG CCTGTCTAGT
    GCCAATGCGG CTGGCCGAAT CCAGCAGCTT CATTTGTCAG AAGATTTAAG CCCTCGGGAA
    ATACAAGAAA ATACTTTCTC TCTTCAAGCA GCCTGCTGTG CGGCCCTCAC GGAGCTGGTG
    CTGGACGACA GCAATGCCCA CCAGGTGGTG CAGGAAAATG GCATATATAC AATAGCAAAA
    TTAATTTTAC CAAACAAACA AAAGAATGCA GCAAAAACTA ATCTATTACA GTGTTACGCA
    TTCAGAGCCT TGAGGTTTCT CTTCAGTATG GAAAGAAACA GACCACTGTT TAAAAGACTT
    TTCCCCACCG ACCTGTTTGA GACCTTCATT GATATAGGAC ATTATGTTCG TGATATCAGT
    GCTTATGAAG AATTAGTATC AAAGTTGAAT TTATTAGTGG AGGATGAACT GAAGCAAATT
    GCTGAAAATG TTGAAAGCAT TAATCAGAAT AAAGCTCCTT CGAAATATAT AGGCAACTAT
    GCGNATTTGG ATCATCTCGG CAGTGGGGCT TTTGGCTGTG TTTACAAGGT TAGAAAGCGC
    AGTGGTCAAA ATCTGTTAGC AATGAAAGAG GTCAACTTAC ATAACCCAGC ATTTGGAAAA
    GATAAAAAAG ACCGAGACAG CAGTGTAAGG AATATTGTTT CTGAATTAAC CATAATTAAA
    GAGCAGCTTT ATCATCCAAA TGTTGTACGC TATTATAAAA CATTTCTGGA AAATGATAGG
    CTGTATATAG TTATGGAACT TATAGAAGGG GCCCCTCTTG GAGAGCATTT CAGTTCTTTG
    AAGGAAAAAC GACATCATTT TGCTGAAGAG AGACTATGGA AAATATTTAT ACAGCTGTGC
    TTGGCTCTTC GGTACTTGCA CAAGGAGAAG AGGATCATCC ATAGAGACCT GACGCCGAAC
    AACATCATGC TGGGGGACAA GGACAGAGTG ACCGTCACTG ACTTTGGCCT GGCAAAGCAA
    AAACAAGAGA GCAGTAGACT CACGTCTGTT GTGGGAACGA TTCTGTACTC CTGCCCGGAG
    GTGCTGAAGA GCGAGCCGTA TGGGGAAAAG GCAGATGTGT GGGCAGCAGG CTGCATCCTT
    TATCAGATGG CGACCCTGGA TCCTCCCTTC TACAGCACCA ACATGCTCTC CCTGGCCACA
    AAAATAGTGG AGGCGGTATA TGAACCAGTG CCAGAAGGCA TCTACTCTGA AAAGGTGATC
    ACTACCATCA GCAGATGCCT CACTCCAGAT GCAGAAACCC GCCCAGATAT TGTAGAAGTC
    AGCTCCATGA TATCAGATAT CATGATGAAG TATGTAGACA ATTTATCCAC ATCCCAGCTG
    GCCTTGGAGA AGAAGCTGGA ACGGGAACGG AGGCGCACAC AGAGGTATTT TATGGAAGCC
    AACCGGAATG CAATCATATG TCAGCAGGAG CCTACTCTGC TGTCTCAGGA AACCTTTGAG
    AAGGCAAGCT TGAGTAGCAG CAGCAGTGGA GCAGGAAGCC TGAAAAGTGA ACTTTCAGAA
    ATCACAGACC TCCCTGCTGA AGGCTTCCAG GCACCATGTG TTAAGGATGA AGACCGGACT
    TGTGATGGAC TCTTATCAGA TGATAACTTC AATTTGGAAA ATATTGAGAA AGATCTATAT
    TCAGAGCTTG ACGATGAATT GGACGTTTCG GATAACTTTA GCAGCTCCGG CTCAAGCCCT
    TTGAAGGAAT CGACATTCAG CACTTTAAAA AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA
    CAATCCCAAA CTAGAGACTT CACTGGTGGA ATAGGATCCA GACTGAGACC AGCTTTGCTG
    CCTCTTGACC TGCTTCTGAA AGTGCCACCC CTCCTGCTCA GTGCCCACGT TAAGGAGGTA
    GAGGCTGAGT TAGTGACGGG GTGGCAGTCC CACAGCCTTC CCACTGTGAT TCTCCACAGT
    CTCAAACACC ATGCATCTGC AGGAATTGCT GTGTCACAGA GGAAAGTACG TCAGATCAGT
    GATCCTATTC AGCAGATCCT AATCCAGCTG CACAAAGTCA TCTACATCAC TCAGCTTCCT
    CCAGCTTTGC ACCACAACTT GAAAAGAAGG ATTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAAGGATCT
    CCAGAACCAA TTGAACCCAA CTTTTTCACA GCAGATTACC ACTTATTGCA TCATTCATTG
    GGTGGAAAGA GCCTGTCCTC AAATGACCCA ACAGGTCTAC CCTCTAGCTT TGATTTGGAG
    GAGGGGATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCTTAGA GGAAAGTGGC
    TATTACAATT TTACTTCTAA CAGGTATCAC TCCTATCCAT GGGGGACCAA GAATTATCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815846.2
    CDS1203..4694
    Translation

    Target ORF information:

    RefSeq Version XM_010817544.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NIMA related kinase 10 (NEK10), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817544.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGACAAAAA AGTGAAGGCC ACTGAAAAAT CAACTGATAA AAAACAGCAA 
    GGAGTCAGCA TCAGGGACTA TTCAGATCTT AAAAGACTTC TGTGCCTTTT GAACGTTCAA
    TCAAGCAAGC AACAGCTTCC AGCCATTAAC TTCGATAGCG TCCAGAACAG CATAATGAAG
    TCTGAGCCTG CCGTCAGGAC AGGTGGGCAC AGAGCTCGAG GTCGGTGGCA TGAGTCCACA
    GAAGCTGTTG AACTCGAAAA TTTTAGTATA AACTACAAGA ATGAGAGAAA TTTCAGCAAA
    CATCCCCAGC ATAAACTATT TCAGGAGATA TTTACAGCTT TGGTGAAAAA TAGACTCATA
    TGCAGAGAGT GGGTTGATCG CGCTCCATCT CTTCATTTTC TGAGAGTGTT AATTTGTCTG
    AGACTCCTCA TGAGGGATCC ATGTTATCAG GAAATACTTC ATAGCTTGGG TGGAATTGAA
    AACCTAGCTC AGTACATGGA GATCGTGGCG CACGGGTACC TCGGCTACGG GGAGGCGCAG
    CACAGCGTGG ACAAGCTGGT GAACATGACG TATATTTTTC AAAAACTTGC TGCTGTCAGA
    GACCAAAGAG AATGGGTCAC CACAAGCGGA GCTCACAAGA CATTAGTGAA TTTACTCAGT
    GCCCGAGATA CGAATGTTCT GCTGGGTGCT CTTCTGGCTC TGGCTAGTTT AGCTGAAAGT
    CCAGAGTGTA GGGAGAAGAT AAGTGAGCTC AACATTGTAG AAAATCTGCT GATGATTTTA
    CACGAATATG ACTTGCTTTC CAAAAGGCTG ACAGCCGAGC TGCTGCGCCT GCTCTGTGCC
    GAGCGCCAGG TGAGAGAGCA GGTGAAGCTC TATGAGGGGG TCCCCGTCCT GCTCAGCCTG
    CTGCACTCAG ACCACCTGAA GCTGCTATGG AGTGTCGTCT GGATTCTGGT GCAGATTTGC
    GAGGACCCAG AGACCAGCGT GGAAATCCGC ATCTGGGGCG GCATCAAGCA ACTTCTTCAT
    ATTTTGCGAG GAGACAGAAA TTTTGTTTCT GATCGTTCCT CCATTGGAAG CCTGTCTAGT
    GCCAATGCGG CTGGCCGAAT CCAGCAGCTT CATTTGTCAG AAGATTTAAG CCCTCGGGAA
    ATACAAGAAA ATACTTTCTC TCTTCAAGCA GCCTGCTGTG CGGCCCTCAC GGAGCTGGTG
    CTGGACGACA GCAATGCCCA CCAGGTGGTG CAGGAAAATG GCATATATAC AATAGCAAAA
    TTAATTTTAC CAAACAAACA AAAGAATGCA GCAAAAACTA ATCTATTACA GTGTTACGCA
    TTCAGAGCCT TGAGGTTTCT CTTCAGTATG GAAAGAAACA GACCACTGTT TAAAAGACTT
    TTCCCCACCG ACCTGTTTGA GACCTTCATT GATATAGGAC ATTATGTTCG TGATATCAGT
    GCTTATGAAG AATTAGTATC AAAGTTGAAT TTATTAGTGG AGGATGAACT GAAGCAAATT
    GCTGAAAATG TTGAAAGCAT TAATCAGAAT AAAGCTCCTT CGAAATATAT AGGCAACTAT
    GCGNATTTGG ATCATCTCGG CAGTGGGGCT TTTGGCTGTG TTTACAAGGT TAGAAAGCGC
    AGTGGTCAAA ATCTGTTAGC AATGAAAGAG GTCAACTTAC ATAACCCAGC ATTTGGAAAA
    GATAAAAAAG ACCGAGACAG CAGTGTAAGG AATATTGTTT CTGAATTAAC CATAATTAAA
    GAGCAGCTTT ATCATCCAAA TGTTGTACGC TATTATAAAA CATTTCTGGA AAATGATAGG
    CTGTATATAG TTATGGAACT TATAGAAGGG GCCCCTCTTG GAGAGCATTT CAGTTCTTTG
    AAGGAAAAAC GACATCATTT TGCTGAAGAG AGACTATGGA AAATATTTAT ACAGCTGTGC
    TTGGCTCTTC GGTACTTGCA CAAGGAGAAG AGGATCATCC ATAGAGACCT GACGCCGAAC
    AACATCATGC TGGGGGACAA GGACAGAGTG ACCGTCACTG ACTTTGGCCT GGCAAAGCAA
    AAACAAGAGA GCAGTAGACT CACGTCTGTT GTGGGAACGA TTCTGTACTC CTGCCCGGAG
    GTGCTGAAGA GCGAGCCGTA TGGGGAAAAG GCAGATGTGT GGGCAGCAGG CTGCATCCTT
    TATCAGATGG CGACCCTGGA TCCTCCCTTC TACAGCACCA ACATGCTCTC CCTGGCCACA
    AAAATAGTGG AGGCGGTATA TGAACCAGTG CCAGAAGGCA TCTACTCTGA AAAGGTGATC
    ACTACCATCA GCAGATGCCT CACTCCAGAT GCAGAAACCC GCCCAGATAT TGTAGAAGTC
    AGCTCCATGA TATCAGATAT CATGATGAAG TATGTAGACA ATTTATCCAC ATCCCAGCTG
    GCCTTGGAGA AGAAGCTGGA ACGGGAACGG AGGCGCACAC AGAGGTATTT TATGGAAGCC
    AACCGGAATG CAATCATATG TCAGCAGGAG CCTACTCTGC TGTCTCAGGA AACCTTTGAG
    AAGGCAAGCT TGAGTAGCAG CAGCAGTGGA GCAGGAAGCC TGAAAAGTGA ACTTTCAGAA
    ATCACAGACC TCCCTGCTGA AGGCTTCCAG GCACCATGTG TTAAGGATGA AGACCGGACT
    TGTGATGGAC TCTTATCAGA TGATAACTTC AATTTGGAAA ATATTGAGAA AGATCTATAT
    TCAGAGCTTG ACGATGAATT GGACGTTTCG GATAACTTTA GCAGCTCCGG CTCAAGCCCT
    TTGAAGGAAT CGACATTCAG CACTTTAAAA AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA
    CAATCCCAAA CTAGAGACTT CACTGGTGGA ATAGGATCCA GACTGAGACC AGCTTTGCTG
    CCTCTTGACC TGCTTCTGAA AGTGCCACCC CTCCTGCTCA GTGCCCACGT TAAGGAGGTA
    GAGGCTGAGT TAGTGACGGG GTGGCAGTCC CACAGCCTTC CCACTGTGAT TCTCCACAGT
    CTCAAACACC ATGCATCTGC AGGAATTGCT GTGTCACAGA GGAAAGTACG TCAGATCAGT
    GATCCTATTC AGCAGATCCT AATCCAGCTG CACAAAGTCA TCTACATCAC TCAGCTTCCT
    CCAGCTTTGC ACCACAACTT GAAAAGAAGG ATTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAAGGATCT
    CCAGAACCAA TTGAACCCAA CTTTTTCACA GCAGATTACC ACTTATTGCA TCATTCATTG
    GGTGGAAAGA GCCTGTCCTC AAATGACCCA ACAGGTCTAC CCTCTAGCTT TGATTTGGAG
    GAGGGGATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCTTAGA GGAAAGTGGC
    TATTACAATT TTACTTCTAA CAGGTATCAC TCCTATCCAT GGGGGACCAA GAATTATCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50538
    Clone ID Related Accession (Same CDS sequence) XM_010817544.2 , XM_015468659.1
    Accession Version XM_010817544.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase Nek10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104540.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817544.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGACAAAAA AGTGAAGGCC ACTGAAAAAT CAACTGATAA AAAACAGCAA 
    GGAGTCACCA TCAGGGACTA TTCAGATCTT AAAAGACTTC TGTGCCTTTT GAACGTTCAA
    TCAAGCAAGC AACAGCTTCC AGCCATTAAC TTCGATAGCG TCCAGAACAG CATAATGAAG
    TCTGAGCCTG CCGTCAGGAC AGGTGGGCAC AGAGCTCGAG GTCGGTGGCA TGAGTCCACA
    GAAGCTGTTG AACTCGAAAA TTTTAGTATA AACTACAAGA ATGAGAGAAA TTTCAGCAAA
    CATCCCCAGC ATAAACTATT TCAGGAGATA TTTACAGCTT TGGTGAAAAA TAGACTCATA
    TGCAGAGAGT GGGTTGATCG CGCTCCATCT CTTCATTTTC TGAGAGTGTT AATTTGTCTG
    AGACTCCTCA TGAGGGATCC ATGTTATCAG GAAATACTTC ATAGCTTGGG TGGAATTGAA
    AACCTAGCTC AGTACATGGA GATCGTGGCG CACGGGTACC TCGGCTACGG GGAGGCGCAG
    CACAGCGTGG ACAAGCTGGT GAACATGACG TATATTTTTC AAAAACTTGC TGCTGTCAGA
    GACCAAAGAG AATGGGTCAC CACAAGCGGA GCTCACAAGA CATTAGTGAA TTTACTCAGT
    GCCCGAGATA CGAATGTTCT GCTGGGTGCT CTTCTGGCTC TGGCTAGTTT AGCTGAAAGT
    CCAGAGTGTA GGGAGAAGAT AAGTGAGCTC AACATTGTAG AAAATCTGCT GATGATTTTA
    CACGAATATG ACTTGCTTTC CAAAAGGCTG ACAGCCGAGC TGCTGCGCCT GCTCTGTGCC
    GAGCGCCAGG TGAGAGAGCA GGTGAAGCTC TATGAGGGGG TCCCCGTCCT GCTCAGCCTG
    CTGCACTCAG ACCACCTGAA GCTGCTATGG AGTGTCGTCT GGATTCTGGT GCAGATTTGC
    GAGGACCCAG AGACCAGCGT GGAAATCCGC ATCTGGGGCG GCATCAAGCA ACTTCTTCAT
    ATTTTGCGAG GAGACAGAAA TTTTGTTTCT GATCGTTCCT CCATTGGAAG CCTGTCTAGT
    GCCAATGCGG CTGGCCGAAT CCAGCAGCTT CATTTGTCAG AAGATTTAAG CCCTCGGGAA
    ATACAAGAAA ATACTTTCTC TCTTCAAGCA GCCTGCTGTG CGGCCCTCAC GGAGCTGGTG
    CTGGACGACA GCAATGCCCA CCAGGTGGTG CAGGAAAATG GCATATATAC AATAGCAAAA
    TTAATTTTAC CAAACAAACA AAAGAATGCA GCAAAAACTA ATCTATTACA GTGTTACGCA
    TTCAGAGCCT TGAGGTTTCT CTTCAGTATG GAAAGAAACA GACCACTGTT TAAAAGACTT
    TTCCCCACCG ACCTGTTTGA GACCTTCATT GATATAGGAC ATTATGTTCG TGATATCAGT
    GCTTATGAAG AATTAGTATC AAAGTTGAAT TTATTAGTGG AGGATGAACT GAAGCAAATT
    GCTGAAAATG TTGAAAGCAT TAATCAGAAT AAAGCTCCTT CGAAATATAT AGGCAACTAT
    GCGATTTTGG ATCATCTCGG CAGTGGGGCT TTTGGCTGTG TTTACAAGGT TAGAAAGCGC
    AGTGGTCAAA ATCTGTTAGC AATGAAAGAG GTCAACTTAC ATAACCCAGC ATTTGGAAAA
    GATAAAAAAG ACCGAGACAG CAGTGTAAGG AATATTGTTT CTGAATTAAC CATAATTAAA
    GAGCAGCTTT ATCATCCAAA TGTTGTACGC TATTATAAAA CATTTCTGGA AAATGATAGG
    CTGTATATAG TTATGGAACT TATAGAAGGG GCCCCTCTTG GAGAGCATTT CAGTTCTTTG
    AAGGAAAAAC GACATCATTT TGCTGAAGAG AGACTATGGA AAATATTTAT ACAGCTGTGC
    TTGGCTCTTC GGTACTTGCA CAAGGAGAAG AGGATCATCC ATAGAGACCT GACGCCGAAC
    AACATCATGC TGGGGGACAA GGACAGAGTG ACCGTCACTG ACTTTGGCCT GGCAAAGCAA
    AAACAAGAGA GCAGTAGACT CACGTCTGTT GTGGGAACGA TTCTGTACTC CTGCCCGGAG
    GTGCTGAAGA GCGAGCCGTA TGGGGAAAAG GCAGATGTGT GGGCAGCAGG CTGCATCCTT
    TATCAGATGG CGACCCTGGA TCCTCCCTTC TACAGCACCA ACATGCTCTC CCTGGCCACA
    AAAATAGTGG AGGCGGTATA TGAACCAGTG CCAGAAGGCA TCTACTCTGA AAAGGTGATC
    ACTACCATCA GCAGATGCCT CACTCCAGAT GCAGAAACCC GCCCAGATAT TGTAGAAGTC
    AGCTCCATGA TATCAGATAT CATGATGAAG TATGTAGACA ATTTATCCAC ATCCCAGCTG
    GCCTTGGAGA AGAAGCTGGA ACGGGAACGG AGGCGCACAC AGAGGTATTT TATGGAAGCC
    AACCGGAATG CAATCATATG TCAGCAGGAG CCTACTCTGC TGTCTCAGGA AACCTTTGAG
    AAGGCAAGCT TGAGTAGCAG CAGCAGTGGA GCAGGAAGCC TGAAAAGTGA ACTTTCAGAA
    ATCACAGACC TCCCTGCTGA AGGCTTCCAG GCACCATGTG TTAAGGATGA AGACCGGACT
    TGTGATGGAC TCTTATCAGA TGATAACTTC AATTTGGAAA ATATTGAGAA AGATCTATAT
    TCAGAGCTTG ACGATGAATT GGACGTTTCG GATAACTTTA GCAGCTCCGG CTCAAGCCCT
    TTGAAGGAAT CGACATTCAG CACTTTAAAA AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA
    CAATCCCAAA CTAGAGACTT CACTGGTGGA ATAGGATCCA GACTGAGACC AGCTTTGCTG
    CCTCTTGACC TGCTTCTGAA AGTGCCACCC CTCCTGCTCA GTGCCCACGT TAAGGAGGTA
    GAGGCTGAGT TAGTGACGGG GTGGCAGTCC CACAGCCTTC CCACTGTGAT TCTCCACAGT
    CTCAAACACC ATGCATCTGC AGGAATTGCT GTGTCACAGA GGAAAGTACG TCAGATCAGT
    GATCCTATTC AGCAGATCCT AATCCAGCTG CACAAAGTCA TCTACATCAC TCAGCTTCCT
    CCAGCTTTGC ACCACAACTT GAAAAGAAGG ATTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAAGGATCT
    CCAGAACCAA TTGAACCCAA CTTTTTCACA GCAGATTACC ACTTATTGCA TCATTCATTG
    GGTGGAAAGA GCCTGTCCTC AAATGACCCA ACAGGTCTAC CCTCTAGCTT TGATTTGGAG
    GAGGGGATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCTTAGA GGAAAGTGGC
    TATTACAATT TTACTTCTAA CAGGTATCAC TCCTATCCAT GGGGGACCAA GAATTATCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815846.1
    CDS334..3825
    Translation

    Target ORF information:

    RefSeq Version XM_010817544.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NIMA related kinase 10 (NEK10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817544.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGACAAAAA AGTGAAGGCC ACTGAAAAAT CAACTGATAA AAAACAGCAA 
    GGAGTCACCA TCAGGGACTA TTCAGATCTT AAAAGACTTC TGTGCCTTTT GAACGTTCAA
    TCAAGCAAGC AACAGCTTCC AGCCATTAAC TTCGATAGCG TCCAGAACAG CATAATGAAG
    TCTGAGCCTG CCGTCAGGAC AGGTGGGCAC AGAGCTCGAG GTCGGTGGCA TGAGTCCACA
    GAAGCTGTTG AACTCGAAAA TTTTAGTATA AACTACAAGA ATGAGAGAAA TTTCAGCAAA
    CATCCCCAGC ATAAACTATT TCAGGAGATA TTTACAGCTT TGGTGAAAAA TAGACTCATA
    TGCAGAGAGT GGGTTGATCG CGCTCCATCT CTTCATTTTC TGAGAGTGTT AATTTGTCTG
    AGACTCCTCA TGAGGGATCC ATGTTATCAG GAAATACTTC ATAGCTTGGG TGGAATTGAA
    AACCTAGCTC AGTACATGGA GATCGTGGCG CACGGGTACC TCGGCTACGG GGAGGCGCAG
    CACAGCGTGG ACAAGCTGGT GAACATGACG TATATTTTTC AAAAACTTGC TGCTGTCAGA
    GACCAAAGAG AATGGGTCAC CACAAGCGGA GCTCACAAGA CATTAGTGAA TTTACTCAGT
    GCCCGAGATA CGAATGTTCT GCTGGGTGCT CTTCTGGCTC TGGCTAGTTT AGCTGAAAGT
    CCAGAGTGTA GGGAGAAGAT AAGTGAGCTC AACATTGTAG AAAATCTGCT GATGATTTTA
    CACGAATATG ACTTGCTTTC CAAAAGGCTG ACAGCCGAGC TGCTGCGCCT GCTCTGTGCC
    GAGCGCCAGG TGAGAGAGCA GGTGAAGCTC TATGAGGGGG TCCCCGTCCT GCTCAGCCTG
    CTGCACTCAG ACCACCTGAA GCTGCTATGG AGTGTCGTCT GGATTCTGGT GCAGATTTGC
    GAGGACCCAG AGACCAGCGT GGAAATCCGC ATCTGGGGCG GCATCAAGCA ACTTCTTCAT
    ATTTTGCGAG GAGACAGAAA TTTTGTTTCT GATCGTTCCT CCATTGGAAG CCTGTCTAGT
    GCCAATGCGG CTGGCCGAAT CCAGCAGCTT CATTTGTCAG AAGATTTAAG CCCTCGGGAA
    ATACAAGAAA ATACTTTCTC TCTTCAAGCA GCCTGCTGTG CGGCCCTCAC GGAGCTGGTG
    CTGGACGACA GCAATGCCCA CCAGGTGGTG CAGGAAAATG GCATATATAC AATAGCAAAA
    TTAATTTTAC CAAACAAACA AAAGAATGCA GCAAAAACTA ATCTATTACA GTGTTACGCA
    TTCAGAGCCT TGAGGTTTCT CTTCAGTATG GAAAGAAACA GACCACTGTT TAAAAGACTT
    TTCCCCACCG ACCTGTTTGA GACCTTCATT GATATAGGAC ATTATGTTCG TGATATCAGT
    GCTTATGAAG AATTAGTATC AAAGTTGAAT TTATTAGTGG AGGATGAACT GAAGCAAATT
    GCTGAAAATG TTGAAAGCAT TAATCAGAAT AAAGCTCCTT CGAAATATAT AGGCAACTAT
    GCGATTTTGG ATCATCTCGG CAGTGGGGCT TTTGGCTGTG TTTACAAGGT TAGAAAGCGC
    AGTGGTCAAA ATCTGTTAGC AATGAAAGAG GTCAACTTAC ATAACCCAGC ATTTGGAAAA
    GATAAAAAAG ACCGAGACAG CAGTGTAAGG AATATTGTTT CTGAATTAAC CATAATTAAA
    GAGCAGCTTT ATCATCCAAA TGTTGTACGC TATTATAAAA CATTTCTGGA AAATGATAGG
    CTGTATATAG TTATGGAACT TATAGAAGGG GCCCCTCTTG GAGAGCATTT CAGTTCTTTG
    AAGGAAAAAC GACATCATTT TGCTGAAGAG AGACTATGGA AAATATTTAT ACAGCTGTGC
    TTGGCTCTTC GGTACTTGCA CAAGGAGAAG AGGATCATCC ATAGAGACCT GACGCCGAAC
    AACATCATGC TGGGGGACAA GGACAGAGTG ACCGTCACTG ACTTTGGCCT GGCAAAGCAA
    AAACAAGAGA GCAGTAGACT CACGTCTGTT GTGGGAACGA TTCTGTACTC CTGCCCGGAG
    GTGCTGAAGA GCGAGCCGTA TGGGGAAAAG GCAGATGTGT GGGCAGCAGG CTGCATCCTT
    TATCAGATGG CGACCCTGGA TCCTCCCTTC TACAGCACCA ACATGCTCTC CCTGGCCACA
    AAAATAGTGG AGGCGGTATA TGAACCAGTG CCAGAAGGCA TCTACTCTGA AAAGGTGATC
    ACTACCATCA GCAGATGCCT CACTCCAGAT GCAGAAACCC GCCCAGATAT TGTAGAAGTC
    AGCTCCATGA TATCAGATAT CATGATGAAG TATGTAGACA ATTTATCCAC ATCCCAGCTG
    GCCTTGGAGA AGAAGCTGGA ACGGGAACGG AGGCGCACAC AGAGGTATTT TATGGAAGCC
    AACCGGAATG CAATCATATG TCAGCAGGAG CCTACTCTGC TGTCTCAGGA AACCTTTGAG
    AAGGCAAGCT TGAGTAGCAG CAGCAGTGGA GCAGGAAGCC TGAAAAGTGA ACTTTCAGAA
    ATCACAGACC TCCCTGCTGA AGGCTTCCAG GCACCATGTG TTAAGGATGA AGACCGGACT
    TGTGATGGAC TCTTATCAGA TGATAACTTC AATTTGGAAA ATATTGAGAA AGATCTATAT
    TCAGAGCTTG ACGATGAATT GGACGTTTCG GATAACTTTA GCAGCTCCGG CTCAAGCCCT
    TTGAAGGAAT CGACATTCAG CACTTTAAAA AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA
    CAATCCCAAA CTAGAGACTT CACTGGTGGA ATAGGATCCA GACTGAGACC AGCTTTGCTG
    CCTCTTGACC TGCTTCTGAA AGTGCCACCC CTCCTGCTCA GTGCCCACGT TAAGGAGGTA
    GAGGCTGAGT TAGTGACGGG GTGGCAGTCC CACAGCCTTC CCACTGTGAT TCTCCACAGT
    CTCAAACACC ATGCATCTGC AGGAATTGCT GTGTCACAGA GGAAAGTACG TCAGATCAGT
    GATCCTATTC AGCAGATCCT AATCCAGCTG CACAAAGTCA TCTACATCAC TCAGCTTCCT
    CCAGCTTTGC ACCACAACTT GAAAAGAAGG ATTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAAGGATCT
    CCAGAACCAA TTGAACCCAA CTTTTTCACA GCAGATTACC ACTTATTGCA TCATTCATTG
    GGTGGAAAGA GCCTGTCCTC AAATGACCCA ACAGGTCTAC CCTCTAGCTT TGATTTGGAG
    GAGGGGATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCTTAGA GGAAAGTGGC
    TATTACAATT TTACTTCTAA CAGGTATCAC TCCTATCCAT GGGGGACCAA GAATTATCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50538
    Clone ID Related Accession (Same CDS sequence) XM_010817544.2 , XM_015468659.1
    Accession Version XM_015468659.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase Nek10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646542.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGACAAAAA AGTGAAGGCC ACTGAAAAAT CAACTGATAA AAAACAGCAA 
    GGAGTCACCA TCAGGGACTA TTCAGATCTT AAAAGACTTC TGTGCCTTTT GAACGTTCAA
    TCAAGCAAGC AACAGCTTCC AGCCATTAAC TTCGATAGCG TCCAGAACAG CATAATGAAG
    TCTGAGCCTG CCGTCAGGAC AGGTGGGCAC AGAGCTCGAG GTCGGTGGCA TGAGTCCACA
    GAAGCTGTTG AACTCGAAAA TTTTAGTATA AACTACAAGA ATGAGAGAAA TTTCAGCAAA
    CATCCCCAGC ATAAACTATT TCAGGAGATA TTTACAGCTT TGGTGAAAAA TAGACTCATA
    TGCAGAGAGT GGGTTGATCG CGCTCCATCT CTTCATTTTC TGAGAGTGTT AATTTGTCTG
    AGACTCCTCA TGAGGGATCC ATGTTATCAG GAAATACTTC ATAGCTTGGG TGGAATTGAA
    AACCTAGCTC AGTACATGGA GATCGTGGCG CACGGGTACC TCGGCTACGG GGAGGCGCAG
    CACAGCGTGG ACAAGCTGGT GAACATGACG TATATTTTTC AAAAACTTGC TGCTGTCAGA
    GACCAAAGAG AATGGGTCAC CACAAGCGGA GCTCACAAGA CATTAGTGAA TTTACTCAGT
    GCCCGAGATA CGAATGTTCT GCTGGGTGCT CTTCTGGCTC TGGCTAGTTT AGCTGAAAGT
    CCAGAGTGTA GGGAGAAGAT AAGTGAGCTC AACATTGTAG AAAATCTGCT GATGATTTTA
    CACGAATATG ACTTGCTTTC CAAAAGGCTG ACAGCCGAGC TGCTGCGCCT GCTCTGTGCC
    GAGCGCCAGG TGAGAGAGCA GGTGAAGCTC TATGAGGGGG TCCCCGTCCT GCTCAGCCTG
    CTGCACTCAG ACCACCTGAA GCTGCTATGG AGTGTCGTCT GGATTCTGGT GCAGATTTGC
    GAGGACCCAG AGACCAGCGT GGAAATCCGC ATCTGGGGCG GCATCAAGCA ACTTCTTCAT
    ATTTTGCGAG GAGACAGAAA TTTTGTTTCT GATCGTTCCT CCATTGGAAG CCTGTCTAGT
    GCCAATGCGG CTGGCCGAAT CCAGCAGCTT CATTTGTCAG AAGATTTAAG CCCTCGGGAA
    ATACAAGAAA ATACTTTCTC TCTTCAAGCA GCCTGCTGTG CGGCCCTCAC GGAGCTGGTG
    CTGGACGACA GCAATGCCCA CCAGGTGGTG CAGGAAAATG GCATATATAC AATAGCAAAA
    TTAATTTTAC CAAACAAACA AAAGAATGCA GCAAAAACTA ATCTATTACA GTGTTACGCA
    TTCAGAGCCT TGAGGTTTCT CTTCAGTATG GAAAGAAACA GACCACTGTT TAAAAGACTT
    TTCCCCACCG ACCTGTTTGA GACCTTCATT GATATAGGAC ATTATGTTCG TGATATCAGT
    GCTTATGAAG AATTAGTATC AAAGTTGAAT TTATTAGTGG AGGATGAACT GAAGCAAATT
    GCTGAAAATG TTGAAAGCAT TAATCAGAAT AAAGCTCCTT CGAAATATAT AGGCAACTAT
    GCGATTTTGG ATCATCTCGG CAGTGGGGCT TTTGGCTGTG TTTACAAGGT TAGAAAGCGC
    AGTGGTCAAA ATCTGTTAGC AATGAAAGAG GTCAACTTAC ATAACCCAGC ATTTGGAAAA
    GATAAAAAAG ACCGAGACAG CAGTGTAAGG AATATTGTTT CTGAATTAAC CATAATTAAA
    GAGCAGCTTT ATCATCCAAA TGTTGTACGC TATTATAAAA CATTTCTGGA AAATGATAGG
    CTGTATATAG TTATGGAACT TATAGAAGGG GCCCCTCTTG GAGAGCATTT CAGTTCTTTG
    AAGGAAAAAC GACATCATTT TGCTGAAGAG AGACTATGGA AAATATTTAT ACAGCTGTGC
    TTGGCTCTTC GGTACTTGCA CAAGGAGAAG AGGATCATCC ATAGAGACCT GACGCCGAAC
    AACATCATGC TGGGGGACAA GGACAGAGTG ACCGTCACTG ACTTTGGCCT GGCAAAGCAA
    AAACAAGAGA GCAGTAGACT CACGTCTGTT GTGGGAACGA TTCTGTACTC CTGCCCGGAG
    GTGCTGAAGA GCGAGCCGTA TGGGGAAAAG GCAGATGTGT GGGCAGCAGG CTGCATCCTT
    TATCAGATGG CGACCCTGGA TCCTCCCTTC TACAGCACCA ACATGCTCTC CCTGGCCACA
    AAAATAGTGG AGGCGGTATA TGAACCAGTG CCAGAAGGCA TCTACTCTGA AAAGGTGATC
    ACTACCATCA GCAGATGCCT CACTCCAGAT GCAGAAACCC GCCCAGATAT TGTAGAAGTC
    AGCTCCATGA TATCAGATAT CATGATGAAG TATGTAGACA ATTTATCCAC ATCCCAGCTG
    GCCTTGGAGA AGAAGCTGGA ACGGGAACGG AGGCGCACAC AGAGGTATTT TATGGAAGCC
    AACCGGAATG CAATCATATG TCAGCAGGAG CCTACTCTGC TGTCTCAGGA AACCTTTGAG
    AAGGCAAGCT TGAGTAGCAG CAGCAGTGGA GCAGGAAGCC TGAAAAGTGA ACTTTCAGAA
    ATCACAGACC TCCCTGCTGA AGGCTTCCAG GCACCATGTG TTAAGGATGA AGACCGGACT
    TGTGATGGAC TCTTATCAGA TGATAACTTC AATTTGGAAA ATATTGAGAA AGATCTATAT
    TCAGAGCTTG ACGATGAATT GGACGTTTCG GATAACTTTA GCAGCTCCGG CTCAAGCCCT
    TTGAAGGAAT CGACATTCAG CACTTTAAAA AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA
    CAATCCCAAA CTAGAGACTT CACTGGTGGA ATAGGATCCA GACTGAGACC AGCTTTGCTG
    CCTCTTGACC TGCTTCTGAA AGTGCCACCC CTCCTGCTCA GTGCCCACGT TAAGGAGGTA
    GAGGCTGAGT TAGTGACGGG GTGGCAGTCC CACAGCCTTC CCACTGTGAT TCTCCACAGT
    CTCAAACACC ATGCATCTGC AGGAATTGCT GTGTCACAGA GGAAAGTACG TCAGATCAGT
    GATCCTATTC AGCAGATCCT AATCCAGCTG CACAAAGTCA TCTACATCAC TCAGCTTCCT
    CCAGCTTTGC ACCACAACTT GAAAAGAAGG ATTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAAGGATCT
    CCAGAACCAA TTGAACCCAA CTTTTTCACA GCAGATTACC ACTTATTGCA TCATTCATTG
    GGTGGAAAGA GCCTGTCCTC AAATGACCCA ACAGGTCTAC CCTCTAGCTT TGATTTGGAG
    GAGGGGATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCTTAGA GGAAAGTGGC
    TATTACAATT TTACTTCTAA CAGGTATCAC TCCTATCCAT GGGGGACCAA GAATTATCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324145.1
    CDS334..3825
    Translation

    Target ORF information:

    RefSeq Version XM_015468659.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NIMA related kinase 10 (NEK10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468659.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGACAAAAA AGTGAAGGCC ACTGAAAAAT CAACTGATAA AAAACAGCAA 
    GGAGTCACCA TCAGGGACTA TTCAGATCTT AAAAGACTTC TGTGCCTTTT GAACGTTCAA
    TCAAGCAAGC AACAGCTTCC AGCCATTAAC TTCGATAGCG TCCAGAACAG CATAATGAAG
    TCTGAGCCTG CCGTCAGGAC AGGTGGGCAC AGAGCTCGAG GTCGGTGGCA TGAGTCCACA
    GAAGCTGTTG AACTCGAAAA TTTTAGTATA AACTACAAGA ATGAGAGAAA TTTCAGCAAA
    CATCCCCAGC ATAAACTATT TCAGGAGATA TTTACAGCTT TGGTGAAAAA TAGACTCATA
    TGCAGAGAGT GGGTTGATCG CGCTCCATCT CTTCATTTTC TGAGAGTGTT AATTTGTCTG
    AGACTCCTCA TGAGGGATCC ATGTTATCAG GAAATACTTC ATAGCTTGGG TGGAATTGAA
    AACCTAGCTC AGTACATGGA GATCGTGGCG CACGGGTACC TCGGCTACGG GGAGGCGCAG
    CACAGCGTGG ACAAGCTGGT GAACATGACG TATATTTTTC AAAAACTTGC TGCTGTCAGA
    GACCAAAGAG AATGGGTCAC CACAAGCGGA GCTCACAAGA CATTAGTGAA TTTACTCAGT
    GCCCGAGATA CGAATGTTCT GCTGGGTGCT CTTCTGGCTC TGGCTAGTTT AGCTGAAAGT
    CCAGAGTGTA GGGAGAAGAT AAGTGAGCTC AACATTGTAG AAAATCTGCT GATGATTTTA
    CACGAATATG ACTTGCTTTC CAAAAGGCTG ACAGCCGAGC TGCTGCGCCT GCTCTGTGCC
    GAGCGCCAGG TGAGAGAGCA GGTGAAGCTC TATGAGGGGG TCCCCGTCCT GCTCAGCCTG
    CTGCACTCAG ACCACCTGAA GCTGCTATGG AGTGTCGTCT GGATTCTGGT GCAGATTTGC
    GAGGACCCAG AGACCAGCGT GGAAATCCGC ATCTGGGGCG GCATCAAGCA ACTTCTTCAT
    ATTTTGCGAG GAGACAGAAA TTTTGTTTCT GATCGTTCCT CCATTGGAAG CCTGTCTAGT
    GCCAATGCGG CTGGCCGAAT CCAGCAGCTT CATTTGTCAG AAGATTTAAG CCCTCGGGAA
    ATACAAGAAA ATACTTTCTC TCTTCAAGCA GCCTGCTGTG CGGCCCTCAC GGAGCTGGTG
    CTGGACGACA GCAATGCCCA CCAGGTGGTG CAGGAAAATG GCATATATAC AATAGCAAAA
    TTAATTTTAC CAAACAAACA AAAGAATGCA GCAAAAACTA ATCTATTACA GTGTTACGCA
    TTCAGAGCCT TGAGGTTTCT CTTCAGTATG GAAAGAAACA GACCACTGTT TAAAAGACTT
    TTCCCCACCG ACCTGTTTGA GACCTTCATT GATATAGGAC ATTATGTTCG TGATATCAGT
    GCTTATGAAG AATTAGTATC AAAGTTGAAT TTATTAGTGG AGGATGAACT GAAGCAAATT
    GCTGAAAATG TTGAAAGCAT TAATCAGAAT AAAGCTCCTT CGAAATATAT AGGCAACTAT
    GCGATTTTGG ATCATCTCGG CAGTGGGGCT TTTGGCTGTG TTTACAAGGT TAGAAAGCGC
    AGTGGTCAAA ATCTGTTAGC AATGAAAGAG GTCAACTTAC ATAACCCAGC ATTTGGAAAA
    GATAAAAAAG ACCGAGACAG CAGTGTAAGG AATATTGTTT CTGAATTAAC CATAATTAAA
    GAGCAGCTTT ATCATCCAAA TGTTGTACGC TATTATAAAA CATTTCTGGA AAATGATAGG
    CTGTATATAG TTATGGAACT TATAGAAGGG GCCCCTCTTG GAGAGCATTT CAGTTCTTTG
    AAGGAAAAAC GACATCATTT TGCTGAAGAG AGACTATGGA AAATATTTAT ACAGCTGTGC
    TTGGCTCTTC GGTACTTGCA CAAGGAGAAG AGGATCATCC ATAGAGACCT GACGCCGAAC
    AACATCATGC TGGGGGACAA GGACAGAGTG ACCGTCACTG ACTTTGGCCT GGCAAAGCAA
    AAACAAGAGA GCAGTAGACT CACGTCTGTT GTGGGAACGA TTCTGTACTC CTGCCCGGAG
    GTGCTGAAGA GCGAGCCGTA TGGGGAAAAG GCAGATGTGT GGGCAGCAGG CTGCATCCTT
    TATCAGATGG CGACCCTGGA TCCTCCCTTC TACAGCACCA ACATGCTCTC CCTGGCCACA
    AAAATAGTGG AGGCGGTATA TGAACCAGTG CCAGAAGGCA TCTACTCTGA AAAGGTGATC
    ACTACCATCA GCAGATGCCT CACTCCAGAT GCAGAAACCC GCCCAGATAT TGTAGAAGTC
    AGCTCCATGA TATCAGATAT CATGATGAAG TATGTAGACA ATTTATCCAC ATCCCAGCTG
    GCCTTGGAGA AGAAGCTGGA ACGGGAACGG AGGCGCACAC AGAGGTATTT TATGGAAGCC
    AACCGGAATG CAATCATATG TCAGCAGGAG CCTACTCTGC TGTCTCAGGA AACCTTTGAG
    AAGGCAAGCT TGAGTAGCAG CAGCAGTGGA GCAGGAAGCC TGAAAAGTGA ACTTTCAGAA
    ATCACAGACC TCCCTGCTGA AGGCTTCCAG GCACCATGTG TTAAGGATGA AGACCGGACT
    TGTGATGGAC TCTTATCAGA TGATAACTTC AATTTGGAAA ATATTGAGAA AGATCTATAT
    TCAGAGCTTG ACGATGAATT GGACGTTTCG GATAACTTTA GCAGCTCCGG CTCAAGCCCT
    TTGAAGGAAT CGACATTCAG CACTTTAAAA AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA
    CAATCCCAAA CTAGAGACTT CACTGGTGGA ATAGGATCCA GACTGAGACC AGCTTTGCTG
    CCTCTTGACC TGCTTCTGAA AGTGCCACCC CTCCTGCTCA GTGCCCACGT TAAGGAGGTA
    GAGGCTGAGT TAGTGACGGG GTGGCAGTCC CACAGCCTTC CCACTGTGAT TCTCCACAGT
    CTCAAACACC ATGCATCTGC AGGAATTGCT GTGTCACAGA GGAAAGTACG TCAGATCAGT
    GATCCTATTC AGCAGATCCT AATCCAGCTG CACAAAGTCA TCTACATCAC TCAGCTTCCT
    CCAGCTTTGC ACCACAACTT GAAAAGAAGG ATTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAAGGATCT
    CCAGAACCAA TTGAACCCAA CTTTTTCACA GCAGATTACC ACTTATTGCA TCATTCATTG
    GGTGGAAAGA GCCTGTCCTC AAATGACCCA ACAGGTCTAC CCTCTAGCTT TGATTTGGAG
    GAGGGGATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCTTAGA GGAAAGTGGC
    TATTACAATT TTACTTCTAA CAGGTATCAC TCCTATCCAT GGGGGACCAA GAATTATCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50539
    Clone ID Related Accession (Same CDS sequence) XM_015468660.1 , XM_010817548.2
    Accession Version XM_015468660.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase Nek10 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646542.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTGATC AAGACAAAAA AGTGAAGGCC ACTGAAAAAT CAACTGATAA AAAACAGCAA 
    GGAGTCACCA TCAGGGACTA TTCAGATCTT AAAAGACTTC TGTGCCTTTT GAACGTTCAA
    TCAAGCAAGC AACAGCTTCC AGCCATTAAC TTCGATAGCG TCCAGAACAG CATAATGAAG
    TCTGAGCCTG CCGTCAGGAC AGGTGGGCAC AGAGCTCGAG GTCGGTGGCA TGAGTCCACA
    GAAGCTGTTG AACTCGAAAA TTTTAGTATA AACTACAAGA ATGAGAGAAA TTTCAGCAAA
    CATCCCCAGC ATAAACTATT TCAGGAGATA TTTACAGCTT TGGTGAAAAA TAGACTCATA
    TGCAGAGAGT GGGTTGATCG CGCTCCATCT CTTCATTTTC TGAGAGTGTT AATTTGTCTG
    AGACTCCTCA TGAGGGATCC ATGTTATCAG GAAATACTTC ATAGCTTGGG TGGAATTGAA
    AACCTAGCTC AGTACATGGA GATCGTGGCG CACGGGTACC TCGGCTACGG GGAGGCGCAG
    CACAGCGTGG ACAAGCTGGT GAACATGACG TATATTTTTC AAAAACTTGC TGCTGTCAGA
    GACCAAAGAG AATGGGTCAC CACAAGCGGA GCTCACAAGA CATTAGTGAA TTTACTCAGT
    GCCCGAGATA CGAATGTTCT GCTGGGTGCT CTTCTGGCTC TGGCTAGTTT AGCTGAAAGT
    CCAGAGTGTA GGGAGAAGAT AAGTGAGCTC AACATTGTAG AAAATCTGCT GATGATTTTA
    CACGAATATG ACTTGCTTTC CAAAAGGCTG ACAGCCGAGC TGCTGCGCCT GCTCTGTGCC
    GAGCGCCAGG TGAGAGAGCA GGTGAAGCTC TATGAGGGGG TCCCCGTCCT GCTCAGCCTG
    CTGCACTCAG ACCACCTGAA GCTGCTATGG AGTGTCGTCT GGATTCTGGT GCAGATTTGC
    GAGGACCCAG AGACCAGCGT GGAAATCCGC ATCTGGGGCG GCATCAAGCA ACTTCTTCAT
    ATTTTGCGAG GAGACAGAAA TTTTGTTTCT GATCGTTCCT CCATTGGAAG CCTGTCTAGT
    GCCAATGCGG CTGGCCGAAT CCAGCAGCTT CATTTGTCAG AAGATTTAAG CCCTCGGGAA
    ATACAAGAAA ATACTTTCTC TCTTCAAGCA GCCTGCTGTG CGGCCCTCAC GGAGCTGGTG
    CTGGACGACA GCAATGCCCA CCAGGTGGTG CAGGAAAATG GCATATATAC AATAGCAAAA
    TTAATTTTAC CAAACAAACA AAAGAATGCA GCAAAAACTA ATCTATTACA GTGTTACGCA
    TTCAGAGCCT TGAGGTTTCT CTTCAGTATG GAAAGAAACA GACCACTGTT TAAAAGACTT
    TTCCCCACCG ACCTGTTTGA GACCTTCATT GATATAGGAC ATTATGTTCG TGATATCAGT
    GCTTATGAAG AATTAGTATC AAAGTTGAAT TTATTAGTGG AGGATGAACT GAAGCAAATT
    GCTGAAAATG TTGAAAGCAT TAATCAGAAT AAAGCTCCTT CGAAATATAT AGGCAACTAT
    GCGATTTTGG ATCATCTCGG CAGTGGGGCT TTTGGCTGTG TTTACAAGGT TAGAAAGCGC
    AGTGGTCAAA ATCTGTTAGC AATGAAAGAG GTCAACTTAC ATAACCCAGC ATTTGGAAAA
    GATAAAAAAG ACCGAGACAG CAGTGTAAGG AATATTGTTT CTGAATTAAC CATAATTAAA
    GAGCAGCTTT ATCATCCAAA TGTTGTACGC TATTATAAAA CATTTCTGGA AAATGATAGG
    CTGTATATAG TTATGGAACT TATAGAAGGG GCCCCTCTTG GAGAGCATTT CAGTTCTTTG
    AAGGAAAAAC GACATCATTT TGCTGAAGAG AGACTATGGA AAATATTTAT ACAGCTGTGC
    TTGGCTCTTC GGTACTTGCA CAAGGAGAAG AGGATCATCC ATAGAGACCT GACGCCGAAC
    AACATCATGC TGGGGGACAA GGACAGAGTG ACCGTCACTG ACTTTGGCCT GGCAAAGCAA
    AAACAAGAGA GCAGTAGACT CACGTCTGTT GTGGGAACGA TTCTGTACTC CTGCCCGGAG
    GTGCTGAAGA GCGAGCCGTA TGGGGAAAAG GCAGATGTGT GGGCAGCAGG CTGCATCCTT
    TATCAGATGG CGACCCTGGA TCCTCCCTTC TACAGCACCA ACATGCTCTC CCTGGCCACA
    AAAATAGTGG AGGCGGTATA TGAACCAGTG CCAGAAGGCA TCTACTCTGA AAAGGTGATC
    ACTACCATCA GCAGATGCCT CACTCCAGAT GCAGAAACCC GCCCAGATAT TGTAGAAGTC
    AGCTCCATGA TATCAGATAT CATGATGAAG TATGTAGACA ATTTATCCAC ATCCCAGCTG
    GCCTTGGAGA AGAAGCTGGA ACGGGAACGG AGGCGCACAC AGAGGTATTT TATGGAAGCC
    AACCGGAATG CAATCATATG TCAGCAGGAG CCTACTCTGC TGTCTCAGGA AACCTTTGAG
    AAGGCAAGCT TGAGTAGCAG CAGCAGTGGA GCAGGAAGCC TGAAAAGTGA ACTTTCAGAA
    ATCACAGACC TCCCTGCTGA AGGCTTCCAG GCACCATGTG TTAAGGATGA AGACCGGACT
    TGTGATGGAC TCTTATCAGA TGATAACTTC AATTTGGAAA ATATTGAGAA AGATCTATAT
    TCAGAGCTTG ACGATGAATT GGACGTTTCG GATAACTTTA GCAGCTCCGG CTCAAGCCCT
    TTGAAGGAAT CGACATTCAG CACTTTAAAA AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA
    CAATCCCAAA CTAGAGACTT CACTGGTGGA ATAGGATCCA GACTGAGACC AGCATCTGCA
    GGAATTGCTG TGTCACAGAG GAAAGTACGT CAGATCAGTG ATCCTATTCA GCAGATCCTA
    ATCCAGCTGC ACAAAGTCAT CTACATCACT CAGCTTCCTC CAGCTTTGCA CCACAACTTG
    AAAAGAAGGA TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT
    AATTTGAAAT CTGAAATTAA AAAGTTATCT CAAGGATCTC CAGAACCAAT TGAACCCAAC
    TTTTTCACAG CAGATTACCA CTTATTGCAT CATTCATTGG GTGGAAAGAG CCTGTCCTCA
    AATGACCCAA CAGGTCTACC CTCTAGCTTT GATTTGGAGG AGGGGATAAC ATATGAACAG
    ATGCAGACTG TGATTGAAGA AGTCTTAGAG GAAAGTGGCT ATTACAATTT TACTTCTAAC
    AGGTATCACT CCTATCCATG GGGGACCAAG AATTATCCAA CCAAAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324146.1
    CDS333..3683
    Translation

    Target ORF information:

    RefSeq Version XM_015468660.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468660.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTGATC AAGACAAAAA AGTGAAGGCC ACTGAAAAAT CAACTGATAA AAAACAGCAA 
    GGAGTCACCA TCAGGGACTA TTCAGATCTT AAAAGACTTC TGTGCCTTTT GAACGTTCAA
    TCAAGCAAGC AACAGCTTCC AGCCATTAAC TTCGATAGCG TCCAGAACAG CATAATGAAG
    TCTGAGCCTG CCGTCAGGAC AGGTGGGCAC AGAGCTCGAG GTCGGTGGCA TGAGTCCACA
    GAAGCTGTTG AACTCGAAAA TTTTAGTATA AACTACAAGA ATGAGAGAAA TTTCAGCAAA
    CATCCCCAGC ATAAACTATT TCAGGAGATA TTTACAGCTT TGGTGAAAAA TAGACTCATA
    TGCAGAGAGT GGGTTGATCG CGCTCCATCT CTTCATTTTC TGAGAGTGTT AATTTGTCTG
    AGACTCCTCA TGAGGGATCC ATGTTATCAG GAAATACTTC ATAGCTTGGG TGGAATTGAA
    AACCTAGCTC AGTACATGGA GATCGTGGCG CACGGGTACC TCGGCTACGG GGAGGCGCAG
    CACAGCGTGG ACAAGCTGGT GAACATGACG TATATTTTTC AAAAACTTGC TGCTGTCAGA
    GACCAAAGAG AATGGGTCAC CACAAGCGGA GCTCACAAGA CATTAGTGAA TTTACTCAGT
    GCCCGAGATA CGAATGTTCT GCTGGGTGCT CTTCTGGCTC TGGCTAGTTT AGCTGAAAGT
    CCAGAGTGTA GGGAGAAGAT AAGTGAGCTC AACATTGTAG AAAATCTGCT GATGATTTTA
    CACGAATATG ACTTGCTTTC CAAAAGGCTG ACAGCCGAGC TGCTGCGCCT GCTCTGTGCC
    GAGCGCCAGG TGAGAGAGCA GGTGAAGCTC TATGAGGGGG TCCCCGTCCT GCTCAGCCTG
    CTGCACTCAG ACCACCTGAA GCTGCTATGG AGTGTCGTCT GGATTCTGGT GCAGATTTGC
    GAGGACCCAG AGACCAGCGT GGAAATCCGC ATCTGGGGCG GCATCAAGCA ACTTCTTCAT
    ATTTTGCGAG GAGACAGAAA TTTTGTTTCT GATCGTTCCT CCATTGGAAG CCTGTCTAGT
    GCCAATGCGG CTGGCCGAAT CCAGCAGCTT CATTTGTCAG AAGATTTAAG CCCTCGGGAA
    ATACAAGAAA ATACTTTCTC TCTTCAAGCA GCCTGCTGTG CGGCCCTCAC GGAGCTGGTG
    CTGGACGACA GCAATGCCCA CCAGGTGGTG CAGGAAAATG GCATATATAC AATAGCAAAA
    TTAATTTTAC CAAACAAACA AAAGAATGCA GCAAAAACTA ATCTATTACA GTGTTACGCA
    TTCAGAGCCT TGAGGTTTCT CTTCAGTATG GAAAGAAACA GACCACTGTT TAAAAGACTT
    TTCCCCACCG ACCTGTTTGA GACCTTCATT GATATAGGAC ATTATGTTCG TGATATCAGT
    GCTTATGAAG AATTAGTATC AAAGTTGAAT TTATTAGTGG AGGATGAACT GAAGCAAATT
    GCTGAAAATG TTGAAAGCAT TAATCAGAAT AAAGCTCCTT CGAAATATAT AGGCAACTAT
    GCGATTTTGG ATCATCTCGG CAGTGGGGCT TTTGGCTGTG TTTACAAGGT TAGAAAGCGC
    AGTGGTCAAA ATCTGTTAGC AATGAAAGAG GTCAACTTAC ATAACCCAGC ATTTGGAAAA
    GATAAAAAAG ACCGAGACAG CAGTGTAAGG AATATTGTTT CTGAATTAAC CATAATTAAA
    GAGCAGCTTT ATCATCCAAA TGTTGTACGC TATTATAAAA CATTTCTGGA AAATGATAGG
    CTGTATATAG TTATGGAACT TATAGAAGGG GCCCCTCTTG GAGAGCATTT CAGTTCTTTG
    AAGGAAAAAC GACATCATTT TGCTGAAGAG AGACTATGGA AAATATTTAT ACAGCTGTGC
    TTGGCTCTTC GGTACTTGCA CAAGGAGAAG AGGATCATCC ATAGAGACCT GACGCCGAAC
    AACATCATGC TGGGGGACAA GGACAGAGTG ACCGTCACTG ACTTTGGCCT GGCAAAGCAA
    AAACAAGAGA GCAGTAGACT CACGTCTGTT GTGGGAACGA TTCTGTACTC CTGCCCGGAG
    GTGCTGAAGA GCGAGCCGTA TGGGGAAAAG GCAGATGTGT GGGCAGCAGG CTGCATCCTT
    TATCAGATGG CGACCCTGGA TCCTCCCTTC TACAGCACCA ACATGCTCTC CCTGGCCACA
    AAAATAGTGG AGGCGGTATA TGAACCAGTG CCAGAAGGCA TCTACTCTGA AAAGGTGATC
    ACTACCATCA GCAGATGCCT CACTCCAGAT GCAGAAACCC GCCCAGATAT TGTAGAAGTC
    AGCTCCATGA TATCAGATAT CATGATGAAG TATGTAGACA ATTTATCCAC ATCCCAGCTG
    GCCTTGGAGA AGAAGCTGGA ACGGGAACGG AGGCGCACAC AGAGGTATTT TATGGAAGCC
    AACCGGAATG CAATCATATG TCAGCAGGAG CCTACTCTGC TGTCTCAGGA AACCTTTGAG
    AAGGCAAGCT TGAGTAGCAG CAGCAGTGGA GCAGGAAGCC TGAAAAGTGA ACTTTCAGAA
    ATCACAGACC TCCCTGCTGA AGGCTTCCAG GCACCATGTG TTAAGGATGA AGACCGGACT
    TGTGATGGAC TCTTATCAGA TGATAACTTC AATTTGGAAA ATATTGAGAA AGATCTATAT
    TCAGAGCTTG ACGATGAATT GGACGTTTCG GATAACTTTA GCAGCTCCGG CTCAAGCCCT
    TTGAAGGAAT CGACATTCAG CACTTTAAAA AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA
    CAATCCCAAA CTAGAGACTT CACTGGTGGA ATAGGATCCA GACTGAGACC AGCATCTGCA
    GGAATTGCTG TGTCACAGAG GAAAGTACGT CAGATCAGTG ATCCTATTCA GCAGATCCTA
    ATCCAGCTGC ACAAAGTCAT CTACATCACT CAGCTTCCTC CAGCTTTGCA CCACAACTTG
    AAAAGAAGGA TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT
    AATTTGAAAT CTGAAATTAA AAAGTTATCT CAAGGATCTC CAGAACCAAT TGAACCCAAC
    TTTTTCACAG CAGATTACCA CTTATTGCAT CATTCATTGG GTGGAAAGAG CCTGTCCTCA
    AATGACCCAA CAGGTCTACC CTCTAGCTTT GATTTGGAGG AGGGGATAAC ATATGAACAG
    ATGCAGACTG TGATTGAAGA AGTCTTAGAG GAAAGTGGCT ATTACAATTT TACTTCTAAC
    AGGTATCACT CCTATCCATG GGGGACCAAG AATTATCCAA CCAAAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50539
    Clone ID Related Accession (Same CDS sequence) XM_015468660.1 , XM_010817548.2
    Accession Version XM_010817548.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase Nek10 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104540.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817548.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTGATC AAGACAAAAA AGTGAAGGCC ACTGAAAAAT CAACTGATAA AAAACAGCAA 
    GGAGTCACCA TCAGGGACTA TTCAGATCTT AAAAGACTTC TGTGCCTTTT GAACGTTCAA
    TCAAGCAAGC AACAGCTTCC AGCCATTAAC TTCGATAGCG TCCAGAACAG CATAATGAAG
    TCTGAGCCTG CCGTCAGGAC AGGTGGGCAC AGAGCTCGAG GTCGGTGGCA TGAGTCCACA
    GAAGCTGTTG AACTCGAAAA TTTTAGTATA AACTACAAGA ATGAGAGAAA TTTCAGCAAA
    CATCCCCAGC ATAAACTATT TCAGGAGATA TTTACAGCTT TGGTGAAAAA TAGACTCATA
    TGCAGAGAGT GGGTTGATCG CGCTCCATCT CTTCATTTTC TGAGAGTGTT AATTTGTCTG
    AGACTCCTCA TGAGGGATCC ATGTTATCAG GAAATACTTC ATAGCTTGGG TGGAATTGAA
    AACCTAGCTC AGTACATGGA GATCGTGGCG CACGGGTACC TCGGCTACGG GGAGGCGCAG
    CACAGCGTGG ACAAGCTGGT GAACATGACG TATATTTTTC AAAAACTTGC TGCTGTCAGA
    GACCAAAGAG AATGGGTCAC CACAAGCGGA GCTCACAAGA CATTAGTGAA TTTACTCAGT
    GCCCGAGATA CGAATGTTCT GCTGGGTGCT CTTCTGGCTC TGGCTAGTTT AGCTGAAAGT
    CCAGAGTGTA GGGAGAAGAT AAGTGAGCTC AACATTGTAG AAAATCTGCT GATGATTTTA
    CACGAATATG ACTTGCTTTC CAAAAGGCTG ACAGCCGAGC TGCTGCGCCT GCTCTGTGCC
    GAGCGCCAGG TGAGAGAGCA GGTGAAGCTC TATGAGGGGG TCCCCGTCCT GCTCAGCCTG
    CTGCACTCAG ACCACCTGAA GCTGCTATGG AGTGTCGTCT GGATTCTGGT GCAGATTTGC
    GAGGACCCAG AGACCAGCGT GGAAATCCGC ATCTGGGGCG GCATCAAGCA ACTTCTTCAT
    ATTTTGCGAG GAGACAGAAA TTTTGTTTCT GATCGTTCCT CCATTGGAAG CCTGTCTAGT
    GCCAATGCGG CTGGCCGAAT CCAGCAGCTT CATTTGTCAG AAGATTTAAG CCCTCGGGAA
    ATACAAGAAA ATACTTTCTC TCTTCAAGCA GCCTGCTGTG CGGCCCTCAC GGAGCTGGTG
    CTGGACGACA GCAATGCCCA CCAGGTGGTG CAGGAAAATG GCATATATAC AATAGCAAAA
    TTAATTTTAC CAAACAAACA AAAGAATGCA GCAAAAACTA ATCTATTACA GTGTTACGCA
    TTCAGAGCCT TGAGGTTTCT CTTCAGTATG GAAAGAAACA GACCACTGTT TAAAAGACTT
    TTCCCCACCG ACCTGTTTGA GACCTTCATT GATATAGGAC ATTATGTTCG TGATATCAGT
    GCTTATGAAG AATTAGTATC AAAGTTGAAT TTATTAGTGG AGGATGAACT GAAGCAAATT
    GCTGAAAATG TTGAAAGCAT TAATCAGAAT AAAGCTCCTT CGAAATATAT AGGCAACTAT
    GCGATTTTGG ATCATCTCGG CAGTGGGGCT TTTGGCTGTG TTTACAAGGT TAGAAAGCGC
    AGTGGTCAAA ATCTGTTAGC AATGAAAGAG GTCAACTTAC ATAACCCAGC ATTTGGAAAA
    GATAAAAAAG ACCGAGACAG CAGTGTAAGG AATATTGTTT CTGAATTAAC CATAATTAAA
    GAGCAGCTTT ATCATCCAAA TGTTGTACGC TATTATAAAA CATTTCTGGA AAATGATAGG
    CTGTATATAG TTATGGAACT TATAGAAGGG GCCCCTCTTG GAGAGCATTT CAGTTCTTTG
    AAGGAAAAAC GACATCATTT TGCTGAAGAG AGACTATGGA AAATATTTAT ACAGCTGTGC
    TTGGCTCTTC GGTACTTGCA CAAGGAGAAG AGGATCATCC ATAGAGACCT GACGCCGAAC
    AACATCATGC TGGGGGACAA GGACAGAGTG ACCGTCACTG ACTTTGGCCT GGCAAAGCAA
    AAACAAGAGA GCAGTAGACT CACGTCTGTT GTGGGAACGA TTCTGTACTC CTGCCCGGAG
    GTGCTGAAGA GCGAGCCGTA TGGGGAAAAG GCAGATGTGT GGGCAGCAGG CTGCATCCTT
    TATCAGATGG CGACCCTGGA TCCTCCCTTC TACAGCACCA ACATGCTCTC CCTGGCCACA
    AAAATAGTGG AGGCGGTATA TGAACCAGTG CCAGAAGGCA TCTACTCTGA AAAGGTGATC
    ACTACCATCA GCAGATGCCT CACTCCAGAT GCAGAAACCC GCCCAGATAT TGTAGAAGTC
    AGCTCCATGA TATCAGATAT CATGATGAAG TATGTAGACA ATTTATCCAC ATCCCAGCTG
    GCCTTGGAGA AGAAGCTGGA ACGGGAACGG AGGCGCACAC AGAGGTATTT TATGGAAGCC
    AACCGGAATG CAATCATATG TCAGCAGGAG CCTACTCTGC TGTCTCAGGA AACCTTTGAG
    AAGGCAAGCT TGAGTAGCAG CAGCAGTGGA GCAGGAAGCC TGAAAAGTGA ACTTTCAGAA
    ATCACAGACC TCCCTGCTGA AGGCTTCCAG GCACCATGTG TTAAGGATGA AGACCGGACT
    TGTGATGGAC TCTTATCAGA TGATAACTTC AATTTGGAAA ATATTGAGAA AGATCTATAT
    TCAGAGCTTG ACGATGAATT GGACGTTTCG GATAACTTTA GCAGCTCCGG CTCAAGCCCT
    TTGAAGGAAT CGACATTCAG CACTTTAAAA AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA
    CAATCCCAAA CTAGAGACTT CACTGGTGGA ATAGGATCCA GACTGAGACC AGCATCTGCA
    GGAATTGCTG TGTCACAGAG GAAAGTACGT CAGATCAGTG ATCCTATTCA GCAGATCCTA
    ATCCAGCTGC ACAAAGTCAT CTACATCACT CAGCTTCCTC CAGCTTTGCA CCACAACTTG
    AAAAGAAGGA TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT
    AATTTGAAAT CTGAAATTAA AAAGTTATCT CAAGGATCTC CAGAACCAAT TGAACCCAAC
    TTTTTCACAG CAGATTACCA CTTATTGCAT CATTCATTGG GTGGAAAGAG CCTGTCCTCA
    AATGACCCAA CAGGTCTACC CTCTAGCTTT GATTTGGAGG AGGGGATAAC ATATGAACAG
    ATGCAGACTG TGATTGAAGA AGTCTTAGAG GAAAGTGGCT ATTACAATTT TACTTCTAAC
    AGGTATCACT CCTATCCATG GGGGACCAAG AATTATCCAA CCAAAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815850.1
    CDS333..3683
    Translation

    Target ORF information:

    RefSeq Version XM_010817548.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817548.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTGATC AAGACAAAAA AGTGAAGGCC ACTGAAAAAT CAACTGATAA AAAACAGCAA 
    GGAGTCACCA TCAGGGACTA TTCAGATCTT AAAAGACTTC TGTGCCTTTT GAACGTTCAA
    TCAAGCAAGC AACAGCTTCC AGCCATTAAC TTCGATAGCG TCCAGAACAG CATAATGAAG
    TCTGAGCCTG CCGTCAGGAC AGGTGGGCAC AGAGCTCGAG GTCGGTGGCA TGAGTCCACA
    GAAGCTGTTG AACTCGAAAA TTTTAGTATA AACTACAAGA ATGAGAGAAA TTTCAGCAAA
    CATCCCCAGC ATAAACTATT TCAGGAGATA TTTACAGCTT TGGTGAAAAA TAGACTCATA
    TGCAGAGAGT GGGTTGATCG CGCTCCATCT CTTCATTTTC TGAGAGTGTT AATTTGTCTG
    AGACTCCTCA TGAGGGATCC ATGTTATCAG GAAATACTTC ATAGCTTGGG TGGAATTGAA
    AACCTAGCTC AGTACATGGA GATCGTGGCG CACGGGTACC TCGGCTACGG GGAGGCGCAG
    CACAGCGTGG ACAAGCTGGT GAACATGACG TATATTTTTC AAAAACTTGC TGCTGTCAGA
    GACCAAAGAG AATGGGTCAC CACAAGCGGA GCTCACAAGA CATTAGTGAA TTTACTCAGT
    GCCCGAGATA CGAATGTTCT GCTGGGTGCT CTTCTGGCTC TGGCTAGTTT AGCTGAAAGT
    CCAGAGTGTA GGGAGAAGAT AAGTGAGCTC AACATTGTAG AAAATCTGCT GATGATTTTA
    CACGAATATG ACTTGCTTTC CAAAAGGCTG ACAGCCGAGC TGCTGCGCCT GCTCTGTGCC
    GAGCGCCAGG TGAGAGAGCA GGTGAAGCTC TATGAGGGGG TCCCCGTCCT GCTCAGCCTG
    CTGCACTCAG ACCACCTGAA GCTGCTATGG AGTGTCGTCT GGATTCTGGT GCAGATTTGC
    GAGGACCCAG AGACCAGCGT GGAAATCCGC ATCTGGGGCG GCATCAAGCA ACTTCTTCAT
    ATTTTGCGAG GAGACAGAAA TTTTGTTTCT GATCGTTCCT CCATTGGAAG CCTGTCTAGT
    GCCAATGCGG CTGGCCGAAT CCAGCAGCTT CATTTGTCAG AAGATTTAAG CCCTCGGGAA
    ATACAAGAAA ATACTTTCTC TCTTCAAGCA GCCTGCTGTG CGGCCCTCAC GGAGCTGGTG
    CTGGACGACA GCAATGCCCA CCAGGTGGTG CAGGAAAATG GCATATATAC AATAGCAAAA
    TTAATTTTAC CAAACAAACA AAAGAATGCA GCAAAAACTA ATCTATTACA GTGTTACGCA
    TTCAGAGCCT TGAGGTTTCT CTTCAGTATG GAAAGAAACA GACCACTGTT TAAAAGACTT
    TTCCCCACCG ACCTGTTTGA GACCTTCATT GATATAGGAC ATTATGTTCG TGATATCAGT
    GCTTATGAAG AATTAGTATC AAAGTTGAAT TTATTAGTGG AGGATGAACT GAAGCAAATT
    GCTGAAAATG TTGAAAGCAT TAATCAGAAT AAAGCTCCTT CGAAATATAT AGGCAACTAT
    GCGATTTTGG ATCATCTCGG CAGTGGGGCT TTTGGCTGTG TTTACAAGGT TAGAAAGCGC
    AGTGGTCAAA ATCTGTTAGC AATGAAAGAG GTCAACTTAC ATAACCCAGC ATTTGGAAAA
    GATAAAAAAG ACCGAGACAG CAGTGTAAGG AATATTGTTT CTGAATTAAC CATAATTAAA
    GAGCAGCTTT ATCATCCAAA TGTTGTACGC TATTATAAAA CATTTCTGGA AAATGATAGG
    CTGTATATAG TTATGGAACT TATAGAAGGG GCCCCTCTTG GAGAGCATTT CAGTTCTTTG
    AAGGAAAAAC GACATCATTT TGCTGAAGAG AGACTATGGA AAATATTTAT ACAGCTGTGC
    TTGGCTCTTC GGTACTTGCA CAAGGAGAAG AGGATCATCC ATAGAGACCT GACGCCGAAC
    AACATCATGC TGGGGGACAA GGACAGAGTG ACCGTCACTG ACTTTGGCCT GGCAAAGCAA
    AAACAAGAGA GCAGTAGACT CACGTCTGTT GTGGGAACGA TTCTGTACTC CTGCCCGGAG
    GTGCTGAAGA GCGAGCCGTA TGGGGAAAAG GCAGATGTGT GGGCAGCAGG CTGCATCCTT
    TATCAGATGG CGACCCTGGA TCCTCCCTTC TACAGCACCA ACATGCTCTC CCTGGCCACA
    AAAATAGTGG AGGCGGTATA TGAACCAGTG CCAGAAGGCA TCTACTCTGA AAAGGTGATC
    ACTACCATCA GCAGATGCCT CACTCCAGAT GCAGAAACCC GCCCAGATAT TGTAGAAGTC
    AGCTCCATGA TATCAGATAT CATGATGAAG TATGTAGACA ATTTATCCAC ATCCCAGCTG
    GCCTTGGAGA AGAAGCTGGA ACGGGAACGG AGGCGCACAC AGAGGTATTT TATGGAAGCC
    AACCGGAATG CAATCATATG TCAGCAGGAG CCTACTCTGC TGTCTCAGGA AACCTTTGAG
    AAGGCAAGCT TGAGTAGCAG CAGCAGTGGA GCAGGAAGCC TGAAAAGTGA ACTTTCAGAA
    ATCACAGACC TCCCTGCTGA AGGCTTCCAG GCACCATGTG TTAAGGATGA AGACCGGACT
    TGTGATGGAC TCTTATCAGA TGATAACTTC AATTTGGAAA ATATTGAGAA AGATCTATAT
    TCAGAGCTTG ACGATGAATT GGACGTTTCG GATAACTTTA GCAGCTCCGG CTCAAGCCCT
    TTGAAGGAAT CGACATTCAG CACTTTAAAA AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA
    CAATCCCAAA CTAGAGACTT CACTGGTGGA ATAGGATCCA GACTGAGACC AGCATCTGCA
    GGAATTGCTG TGTCACAGAG GAAAGTACGT CAGATCAGTG ATCCTATTCA GCAGATCCTA
    ATCCAGCTGC ACAAAGTCAT CTACATCACT CAGCTTCCTC CAGCTTTGCA CCACAACTTG
    AAAAGAAGGA TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT
    AATTTGAAAT CTGAAATTAA AAAGTTATCT CAAGGATCTC CAGAACCAAT TGAACCCAAC
    TTTTTCACAG CAGATTACCA CTTATTGCAT CATTCATTGG GTGGAAAGAG CCTGTCCTCA
    AATGACCCAA CAGGTCTACC CTCTAGCTTT GATTTGGAGG AGGGGATAAC ATATGAACAG
    ATGCAGACTG TGATTGAAGA AGTCTTAGAG GAAAGTGGCT ATTACAATTT TACTTCTAAC
    AGGTATCACT CCTATCCATG GGGGACCAAG AATTATCCAA CCAAAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50540
    Clone ID Related Accession (Same CDS sequence) XM_002707776.5 , XM_015468661.1
    Accession Version XM_002707776.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase Nek10 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104540.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002707776.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGTCTG AGCCTGCCGT CAGGACAGGT GGGCACAGAG CTCGAGGTCG GTGGCATGAG 
    TCCACAGAAG CTGTTGAACT CGAAAATTTT AGTATAAACT ACAAGAATGA GAGAAATTTC
    AGCAAACATC CCCAGCATAA ACTATTTCAG GAGATATTTA CAGCTTTGGT GAAAAATAGA
    CTCATATGCA GAGAGTGGGT TGATCGCGCT CCATCTCTTC ATTTTCTGAG AGTGTTAATT
    TGTCTGAGAC TCCTCATGAG GGATCCATGT TATCAGGAAA TACTTCATAG CTTGGGTGGA
    ATTGAAAACC TAGCTCAGTA CATGGAGATC GTGGCGCACG GGTACCTCGG CTACGGGGAG
    GCGCAGCACA GCGTGGACAA GCTGGTGAAC ATGACGTATA TTTTTCAAAA ACTTGCTGCT
    GTCAGAGACC AAAGAGAATG GGTCACCACA AGCGGAGCTC ACAAGACATT AGTGAATTTA
    CTCAGTGCCC GAGATACGAA TGTTCTGCTG GGTGCTCTTC TGGCTCTGGC TAGTTTAGCT
    GAAAGTCCAG AGTGTAGGGA GAAGATAAGT GAGCTCAACA TTGTAGAAAA TCTGCTGATG
    ATTTTACACG AATATGACTT GCTTTCCAAA AGGCTGACAG CCGAGCTGCT GCGCCTGCTC
    TGTGCCGAGC GCCAGGTGAG AGAGCAGGTG AAGCTCTATG AGGGGGTCCC CGTCCTGCTC
    AGCCTGCTGC ACTCAGACCA CCTGAAGCTG CTATGGAGTG TCGTCTGGAT TCTGGTGCAG
    ATTTGCGAGG ACCCAGAGAC CAGCGTGGAA ATCCGCATCT GGGGCGGCAT CAAGCAACTT
    CTTCATATTT TGCGAGGAGA CAGAAATTTT GTTTCTGATC GTTCCTCCAT TGGAAGCCTG
    TCTAGTGCCA ATGCGGCTGG CCGAATCCAG CAGCTTCATT TGTCAGAAGA TTTAAGCCCT
    CGGGAAATAC AAGAAAATAC TTTCTCTCTT CAAGCAGCCT GCTGTGCGGC CCTCACGGAG
    CTGGTGCTGG ACGACAGCAA TGCCCACCAG GTGGTGCAGG AAAATGGCAT ATATACAATA
    GCAAAATTAA TTTTACCAAA CAAACAAAAG AATGCAGCAA AAACTAATCT ATTACAGTGT
    TACGCATTCA GAGCCTTGAG GTTTCTCTTC AGTATGGAAA GAAACAGACC ACTGTTTAAA
    AGACTTTTCC CCACCGACCT GTTTGAGACC TTCATTGATA TAGGACATTA TGTTCGTGAT
    ATCAGTGCTT ATGAAGAATT AGTATCAAAG TTGAATTTAT TAGTGGAGGA TGAACTGAAG
    CAAATTGCTG AAAATGTTGA AAGCATTAAT CAGAATAAAG CTCCTTCGAA ATATATAGGC
    AACTATGCGA TTTTGGATCA TCTCGGCAGT GGGGCTTTTG GCTGTGTTTA CAAGGTTAGA
    AAGCGCAGTG GTCAAAATCT GTTAGCAATG AAAGAGGTCA ACTTACATAA CCCAGCATTT
    GGAAAAGATA AAAAAGACCG AGACAGCAGT GTAAGGAATA TTGTTTCTGA ATTAACCATA
    ATTAAAGAGC AGCTTTATCA TCCAAATGTT GTACGCTATT ATAAAACATT TCTGGAAAAT
    GATAGGCTGT ATATAGTTAT GGAACTTATA GAAGGGGCCC CTCTTGGAGA GCATTTCAGT
    TCTTTGAAGG AAAAACGACA TCATTTTGCT GAAGAGAGAC TATGGAAAAT ATTTATACAG
    CTGTGCTTGG CTCTTCGGTA CTTGCACAAG GAGAAGAGGA TCATCCATAG AGACCTGACG
    CCGAACAACA TCATGCTGGG GGACAAGGAC AGAGTGACCG TCACTGACTT TGGCCTGGCA
    AAGCAAAAAC AAGAGAGCAG TAGACTCACG TCTGTTGTGG GAACGATTCT GTACTCCTGC
    CCGGAGGTGC TGAAGAGCGA GCCGTATGGG GAAAAGGCAG ATGTGTGGGC AGCAGGCTGC
    ATCCTTTATC AGATGGCGAC CCTGGATCCT CCCTTCTACA GCACCAACAT GCTCTCCCTG
    GCCACAAAAA TAGTGGAGGC GGTATATGAA CCAGTGCCAG AAGGCATCTA CTCTGAAAAG
    GTGATCACTA CCATCAGCAG ATGCCTCACT CCAGATGCAG AAACCCGCCC AGATATTGTA
    GAAGTCAGCT CCATGATATC AGATATCATG ATGAAGTATG TAGACAATTT ATCCACATCC
    CAGCTGGCCT TGGAGAAGAA GCTGGAACGG GAACGGAGGC GCACACAGAG GTATTTTATG
    GAAGCCAACC GGAATGCAAT CATATGTCAG CAGGAGCCTA CTCTGCTGTC TCAGGAAACC
    TTTGAGAAGG CAAGCTTGAG TAGCAGCAGC AGTGGAGCAG GAAGCCTGAA AAGTGAACTT
    TCAGAAATCA CAGACCTCCC TGCTGAAGGC TTCCAGGCAC CATGTGTTAA GGATGAAGAC
    CGGACTTGTG ATGGACTCTT ATCAGATGAT AACTTCAATT TGGAAAATAT TGAGAAAGAT
    CTATATTCAG AGCTTGACGA TGAATTGGAC GTTTCGGATA ACTTTAGCAG CTCCGGCTCA
    AGCCCTTTGA AGGAATCGAC ATTCAGCACT TTAAAAAGAA GTTTTAGTGC TTCAGGAGGA
    GAAAGACAAT CCCAAACTAG AGACTTCACT GGTGGAATAG GATCCAGACT GAGACCAGCT
    TTGCTGCCTC TTGACCTGCT TCTGAAAGTG CCACCCCTCC TGCTCAGTGC CCACGTTAAG
    GAGGTAGAGG CTGAGTTAGT GACGGGGTGG CAGTCCCACA GCCTTCCCAC TGTGATTCTC
    CACAGTCTCA AACACCATGC ATCTGCAGGA ATTGCTGTGT CACAGAGGAA AGTACGTCAG
    ATCAGTGATC CTATTCAGCA GATCCTAATC CAGCTGCACA AAGTCATCTA CATCACTCAG
    CTTCCTCCAG CTTTGCACCA CAACTTGAAA AGAAGGATTA TAGAGAGATT CAAGAAATCC
    CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAA
    GGATCTCCAG AACCAATTGA ACCCAACTTT TTCACAGCAG ATTACCACTT ATTGCATCAT
    TCATTGGGTG GAAAGAGCCT GTCCTCAAAT GACCCAACAG GTCTACCCTC TAGCTTTGAT
    TTGGAGGAGG GGATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CTTAGAGGAA
    AGTGGCTATT ACAATTTTAC TTCTAACAGG TATCACTCCT ATCCATGGGG GACCAAGAAT
    TATCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002707822.4
    CDS322..3639
    Translation

    Target ORF information:

    RefSeq Version XM_002707776.5
    Organism Bos taurus(cattle)
    Definition Bos taurus NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002707776.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGTCTG AGCCTGCCGT CAGGACAGGT GGGCACAGAG CTCGAGGTCG GTGGCATGAG 
    TCCACAGAAG CTGTTGAACT CGAAAATTTT AGTATAAACT ACAAGAATGA GAGAAATTTC
    AGCAAACATC CCCAGCATAA ACTATTTCAG GAGATATTTA CAGCTTTGGT GAAAAATAGA
    CTCATATGCA GAGAGTGGGT TGATCGCGCT CCATCTCTTC ATTTTCTGAG AGTGTTAATT
    TGTCTGAGAC TCCTCATGAG GGATCCATGT TATCAGGAAA TACTTCATAG CTTGGGTGGA
    ATTGAAAACC TAGCTCAGTA CATGGAGATC GTGGCGCACG GGTACCTCGG CTACGGGGAG
    GCGCAGCACA GCGTGGACAA GCTGGTGAAC ATGACGTATA TTTTTCAAAA ACTTGCTGCT
    GTCAGAGACC AAAGAGAATG GGTCACCACA AGCGGAGCTC ACAAGACATT AGTGAATTTA
    CTCAGTGCCC GAGATACGAA TGTTCTGCTG GGTGCTCTTC TGGCTCTGGC TAGTTTAGCT
    GAAAGTCCAG AGTGTAGGGA GAAGATAAGT GAGCTCAACA TTGTAGAAAA TCTGCTGATG
    ATTTTACACG AATATGACTT GCTTTCCAAA AGGCTGACAG CCGAGCTGCT GCGCCTGCTC
    TGTGCCGAGC GCCAGGTGAG AGAGCAGGTG AAGCTCTATG AGGGGGTCCC CGTCCTGCTC
    AGCCTGCTGC ACTCAGACCA CCTGAAGCTG CTATGGAGTG TCGTCTGGAT TCTGGTGCAG
    ATTTGCGAGG ACCCAGAGAC CAGCGTGGAA ATCCGCATCT GGGGCGGCAT CAAGCAACTT
    CTTCATATTT TGCGAGGAGA CAGAAATTTT GTTTCTGATC GTTCCTCCAT TGGAAGCCTG
    TCTAGTGCCA ATGCGGCTGG CCGAATCCAG CAGCTTCATT TGTCAGAAGA TTTAAGCCCT
    CGGGAAATAC AAGAAAATAC TTTCTCTCTT CAAGCAGCCT GCTGTGCGGC CCTCACGGAG
    CTGGTGCTGG ACGACAGCAA TGCCCACCAG GTGGTGCAGG AAAATGGCAT ATATACAATA
    GCAAAATTAA TTTTACCAAA CAAACAAAAG AATGCAGCAA AAACTAATCT ATTACAGTGT
    TACGCATTCA GAGCCTTGAG GTTTCTCTTC AGTATGGAAA GAAACAGACC ACTGTTTAAA
    AGACTTTTCC CCACCGACCT GTTTGAGACC TTCATTGATA TAGGACATTA TGTTCGTGAT
    ATCAGTGCTT ATGAAGAATT AGTATCAAAG TTGAATTTAT TAGTGGAGGA TGAACTGAAG
    CAAATTGCTG AAAATGTTGA AAGCATTAAT CAGAATAAAG CTCCTTCGAA ATATATAGGC
    AACTATGCGA TTTTGGATCA TCTCGGCAGT GGGGCTTTTG GCTGTGTTTA CAAGGTTAGA
    AAGCGCAGTG GTCAAAATCT GTTAGCAATG AAAGAGGTCA ACTTACATAA CCCAGCATTT
    GGAAAAGATA AAAAAGACCG AGACAGCAGT GTAAGGAATA TTGTTTCTGA ATTAACCATA
    ATTAAAGAGC AGCTTTATCA TCCAAATGTT GTACGCTATT ATAAAACATT TCTGGAAAAT
    GATAGGCTGT ATATAGTTAT GGAACTTATA GAAGGGGCCC CTCTTGGAGA GCATTTCAGT
    TCTTTGAAGG AAAAACGACA TCATTTTGCT GAAGAGAGAC TATGGAAAAT ATTTATACAG
    CTGTGCTTGG CTCTTCGGTA CTTGCACAAG GAGAAGAGGA TCATCCATAG AGACCTGACG
    CCGAACAACA TCATGCTGGG GGACAAGGAC AGAGTGACCG TCACTGACTT TGGCCTGGCA
    AAGCAAAAAC AAGAGAGCAG TAGACTCACG TCTGTTGTGG GAACGATTCT GTACTCCTGC
    CCGGAGGTGC TGAAGAGCGA GCCGTATGGG GAAAAGGCAG ATGTGTGGGC AGCAGGCTGC
    ATCCTTTATC AGATGGCGAC CCTGGATCCT CCCTTCTACA GCACCAACAT GCTCTCCCTG
    GCCACAAAAA TAGTGGAGGC GGTATATGAA CCAGTGCCAG AAGGCATCTA CTCTGAAAAG
    GTGATCACTA CCATCAGCAG ATGCCTCACT CCAGATGCAG AAACCCGCCC AGATATTGTA
    GAAGTCAGCT CCATGATATC AGATATCATG ATGAAGTATG TAGACAATTT ATCCACATCC
    CAGCTGGCCT TGGAGAAGAA GCTGGAACGG GAACGGAGGC GCACACAGAG GTATTTTATG
    GAAGCCAACC GGAATGCAAT CATATGTCAG CAGGAGCCTA CTCTGCTGTC TCAGGAAACC
    TTTGAGAAGG CAAGCTTGAG TAGCAGCAGC AGTGGAGCAG GAAGCCTGAA AAGTGAACTT
    TCAGAAATCA CAGACCTCCC TGCTGAAGGC TTCCAGGCAC CATGTGTTAA GGATGAAGAC
    CGGACTTGTG ATGGACTCTT ATCAGATGAT AACTTCAATT TGGAAAATAT TGAGAAAGAT
    CTATATTCAG AGCTTGACGA TGAATTGGAC GTTTCGGATA ACTTTAGCAG CTCCGGCTCA
    AGCCCTTTGA AGGAATCGAC ATTCAGCACT TTAAAAAGAA GTTTTAGTGC TTCAGGAGGA
    GAAAGACAAT CCCAAACTAG AGACTTCACT GGTGGAATAG GATCCAGACT GAGACCAGCT
    TTGCTGCCTC TTGACCTGCT TCTGAAAGTG CCACCCCTCC TGCTCAGTGC CCACGTTAAG
    GAGGTAGAGG CTGAGTTAGT GACGGGGTGG CAGTCCCACA GCCTTCCCAC TGTGATTCTC
    CACAGTCTCA AACACCATGC ATCTGCAGGA ATTGCTGTGT CACAGAGGAA AGTACGTCAG
    ATCAGTGATC CTATTCAGCA GATCCTAATC CAGCTGCACA AAGTCATCTA CATCACTCAG
    CTTCCTCCAG CTTTGCACCA CAACTTGAAA AGAAGGATTA TAGAGAGATT CAAGAAATCC
    CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAA
    GGATCTCCAG AACCAATTGA ACCCAACTTT TTCACAGCAG ATTACCACTT ATTGCATCAT
    TCATTGGGTG GAAAGAGCCT GTCCTCAAAT GACCCAACAG GTCTACCCTC TAGCTTTGAT
    TTGGAGGAGG GGATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CTTAGAGGAA
    AGTGGCTATT ACAATTTTAC TTCTAACAGG TATCACTCCT ATCCATGGGG GACCAAGAAT
    TATCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50540
    Clone ID Related Accession (Same CDS sequence) XM_002707776.5 , XM_015468661.1
    Accession Version XM_015468661.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase Nek10 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646542.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGTCTG AGCCTGCCGT CAGGACAGGT GGGCACAGAG CTCGAGGTCG GTGGCATGAG 
    TCCACAGAAG CTGTTGAACT CGAAAATTTT AGTATAAACT ACAAGAATGA GAGAAATTTC
    AGCAAACATC CCCAGCATAA ACTATTTCAG GAGATATTTA CAGCTTTGGT GAAAAATAGA
    CTCATATGCA GAGAGTGGGT TGATCGCGCT CCATCTCTTC ATTTTCTGAG AGTGTTAATT
    TGTCTGAGAC TCCTCATGAG GGATCCATGT TATCAGGAAA TACTTCATAG CTTGGGTGGA
    ATTGAAAACC TAGCTCAGTA CATGGAGATC GTGGCGCACG GGTACCTCGG CTACGGGGAG
    GCGCAGCACA GCGTGGACAA GCTGGTGAAC ATGACGTATA TTTTTCAAAA ACTTGCTGCT
    GTCAGAGACC AAAGAGAATG GGTCACCACA AGCGGAGCTC ACAAGACATT AGTGAATTTA
    CTCAGTGCCC GAGATACGAA TGTTCTGCTG GGTGCTCTTC TGGCTCTGGC TAGTTTAGCT
    GAAAGTCCAG AGTGTAGGGA GAAGATAAGT GAGCTCAACA TTGTAGAAAA TCTGCTGATG
    ATTTTACACG AATATGACTT GCTTTCCAAA AGGCTGACAG CCGAGCTGCT GCGCCTGCTC
    TGTGCCGAGC GCCAGGTGAG AGAGCAGGTG AAGCTCTATG AGGGGGTCCC CGTCCTGCTC
    AGCCTGCTGC ACTCAGACCA CCTGAAGCTG CTATGGAGTG TCGTCTGGAT TCTGGTGCAG
    ATTTGCGAGG ACCCAGAGAC CAGCGTGGAA ATCCGCATCT GGGGCGGCAT CAAGCAACTT
    CTTCATATTT TGCGAGGAGA CAGAAATTTT GTTTCTGATC GTTCCTCCAT TGGAAGCCTG
    TCTAGTGCCA ATGCGGCTGG CCGAATCCAG CAGCTTCATT TGTCAGAAGA TTTAAGCCCT
    CGGGAAATAC AAGAAAATAC TTTCTCTCTT CAAGCAGCCT GCTGTGCGGC CCTCACGGAG
    CTGGTGCTGG ACGACAGCAA TGCCCACCAG GTGGTGCAGG AAAATGGCAT ATATACAATA
    GCAAAATTAA TTTTACCAAA CAAACAAAAG AATGCAGCAA AAACTAATCT ATTACAGTGT
    TACGCATTCA GAGCCTTGAG GTTTCTCTTC AGTATGGAAA GAAACAGACC ACTGTTTAAA
    AGACTTTTCC CCACCGACCT GTTTGAGACC TTCATTGATA TAGGACATTA TGTTCGTGAT
    ATCAGTGCTT ATGAAGAATT AGTATCAAAG TTGAATTTAT TAGTGGAGGA TGAACTGAAG
    CAAATTGCTG AAAATGTTGA AAGCATTAAT CAGAATAAAG CTCCTTCGAA ATATATAGGC
    AACTATGCGA TTTTGGATCA TCTCGGCAGT GGGGCTTTTG GCTGTGTTTA CAAGGTTAGA
    AAGCGCAGTG GTCAAAATCT GTTAGCAATG AAAGAGGTCA ACTTACATAA CCCAGCATTT
    GGAAAAGATA AAAAAGACCG AGACAGCAGT GTAAGGAATA TTGTTTCTGA ATTAACCATA
    ATTAAAGAGC AGCTTTATCA TCCAAATGTT GTACGCTATT ATAAAACATT TCTGGAAAAT
    GATAGGCTGT ATATAGTTAT GGAACTTATA GAAGGGGCCC CTCTTGGAGA GCATTTCAGT
    TCTTTGAAGG AAAAACGACA TCATTTTGCT GAAGAGAGAC TATGGAAAAT ATTTATACAG
    CTGTGCTTGG CTCTTCGGTA CTTGCACAAG GAGAAGAGGA TCATCCATAG AGACCTGACG
    CCGAACAACA TCATGCTGGG GGACAAGGAC AGAGTGACCG TCACTGACTT TGGCCTGGCA
    AAGCAAAAAC AAGAGAGCAG TAGACTCACG TCTGTTGTGG GAACGATTCT GTACTCCTGC
    CCGGAGGTGC TGAAGAGCGA GCCGTATGGG GAAAAGGCAG ATGTGTGGGC AGCAGGCTGC
    ATCCTTTATC AGATGGCGAC CCTGGATCCT CCCTTCTACA GCACCAACAT GCTCTCCCTG
    GCCACAAAAA TAGTGGAGGC GGTATATGAA CCAGTGCCAG AAGGCATCTA CTCTGAAAAG
    GTGATCACTA CCATCAGCAG ATGCCTCACT CCAGATGCAG AAACCCGCCC AGATATTGTA
    GAAGTCAGCT CCATGATATC AGATATCATG ATGAAGTATG TAGACAATTT ATCCACATCC
    CAGCTGGCCT TGGAGAAGAA GCTGGAACGG GAACGGAGGC GCACACAGAG GTATTTTATG
    GAAGCCAACC GGAATGCAAT CATATGTCAG CAGGAGCCTA CTCTGCTGTC TCAGGAAACC
    TTTGAGAAGG CAAGCTTGAG TAGCAGCAGC AGTGGAGCAG GAAGCCTGAA AAGTGAACTT
    TCAGAAATCA CAGACCTCCC TGCTGAAGGC TTCCAGGCAC CATGTGTTAA GGATGAAGAC
    CGGACTTGTG ATGGACTCTT ATCAGATGAT AACTTCAATT TGGAAAATAT TGAGAAAGAT
    CTATATTCAG AGCTTGACGA TGAATTGGAC GTTTCGGATA ACTTTAGCAG CTCCGGCTCA
    AGCCCTTTGA AGGAATCGAC ATTCAGCACT TTAAAAAGAA GTTTTAGTGC TTCAGGAGGA
    GAAAGACAAT CCCAAACTAG AGACTTCACT GGTGGAATAG GATCCAGACT GAGACCAGCT
    TTGCTGCCTC TTGACCTGCT TCTGAAAGTG CCACCCCTCC TGCTCAGTGC CCACGTTAAG
    GAGGTAGAGG CTGAGTTAGT GACGGGGTGG CAGTCCCACA GCCTTCCCAC TGTGATTCTC
    CACAGTCTCA AACACCATGC ATCTGCAGGA ATTGCTGTGT CACAGAGGAA AGTACGTCAG
    ATCAGTGATC CTATTCAGCA GATCCTAATC CAGCTGCACA AAGTCATCTA CATCACTCAG
    CTTCCTCCAG CTTTGCACCA CAACTTGAAA AGAAGGATTA TAGAGAGATT CAAGAAATCC
    CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAA
    GGATCTCCAG AACCAATTGA ACCCAACTTT TTCACAGCAG ATTACCACTT ATTGCATCAT
    TCATTGGGTG GAAAGAGCCT GTCCTCAAAT GACCCAACAG GTCTACCCTC TAGCTTTGAT
    TTGGAGGAGG GGATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CTTAGAGGAA
    AGTGGCTATT ACAATTTTAC TTCTAACAGG TATCACTCCT ATCCATGGGG GACCAAGAAT
    TATCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324147.1
    CDS322..3639
    Translation

    Target ORF information:

    RefSeq Version XM_015468661.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468661.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGTCTG AGCCTGCCGT CAGGACAGGT GGGCACAGAG CTCGAGGTCG GTGGCATGAG 
    TCCACAGAAG CTGTTGAACT CGAAAATTTT AGTATAAACT ACAAGAATGA GAGAAATTTC
    AGCAAACATC CCCAGCATAA ACTATTTCAG GAGATATTTA CAGCTTTGGT GAAAAATAGA
    CTCATATGCA GAGAGTGGGT TGATCGCGCT CCATCTCTTC ATTTTCTGAG AGTGTTAATT
    TGTCTGAGAC TCCTCATGAG GGATCCATGT TATCAGGAAA TACTTCATAG CTTGGGTGGA
    ATTGAAAACC TAGCTCAGTA CATGGAGATC GTGGCGCACG GGTACCTCGG CTACGGGGAG
    GCGCAGCACA GCGTGGACAA GCTGGTGAAC ATGACGTATA TTTTTCAAAA ACTTGCTGCT
    GTCAGAGACC AAAGAGAATG GGTCACCACA AGCGGAGCTC ACAAGACATT AGTGAATTTA
    CTCAGTGCCC GAGATACGAA TGTTCTGCTG GGTGCTCTTC TGGCTCTGGC TAGTTTAGCT
    GAAAGTCCAG AGTGTAGGGA GAAGATAAGT GAGCTCAACA TTGTAGAAAA TCTGCTGATG
    ATTTTACACG AATATGACTT GCTTTCCAAA AGGCTGACAG CCGAGCTGCT GCGCCTGCTC
    TGTGCCGAGC GCCAGGTGAG AGAGCAGGTG AAGCTCTATG AGGGGGTCCC CGTCCTGCTC
    AGCCTGCTGC ACTCAGACCA CCTGAAGCTG CTATGGAGTG TCGTCTGGAT TCTGGTGCAG
    ATTTGCGAGG ACCCAGAGAC CAGCGTGGAA ATCCGCATCT GGGGCGGCAT CAAGCAACTT
    CTTCATATTT TGCGAGGAGA CAGAAATTTT GTTTCTGATC GTTCCTCCAT TGGAAGCCTG
    TCTAGTGCCA ATGCGGCTGG CCGAATCCAG CAGCTTCATT TGTCAGAAGA TTTAAGCCCT
    CGGGAAATAC AAGAAAATAC TTTCTCTCTT CAAGCAGCCT GCTGTGCGGC CCTCACGGAG
    CTGGTGCTGG ACGACAGCAA TGCCCACCAG GTGGTGCAGG AAAATGGCAT ATATACAATA
    GCAAAATTAA TTTTACCAAA CAAACAAAAG AATGCAGCAA AAACTAATCT ATTACAGTGT
    TACGCATTCA GAGCCTTGAG GTTTCTCTTC AGTATGGAAA GAAACAGACC ACTGTTTAAA
    AGACTTTTCC CCACCGACCT GTTTGAGACC TTCATTGATA TAGGACATTA TGTTCGTGAT
    ATCAGTGCTT ATGAAGAATT AGTATCAAAG TTGAATTTAT TAGTGGAGGA TGAACTGAAG
    CAAATTGCTG AAAATGTTGA AAGCATTAAT CAGAATAAAG CTCCTTCGAA ATATATAGGC
    AACTATGCGA TTTTGGATCA TCTCGGCAGT GGGGCTTTTG GCTGTGTTTA CAAGGTTAGA
    AAGCGCAGTG GTCAAAATCT GTTAGCAATG AAAGAGGTCA ACTTACATAA CCCAGCATTT
    GGAAAAGATA AAAAAGACCG AGACAGCAGT GTAAGGAATA TTGTTTCTGA ATTAACCATA
    ATTAAAGAGC AGCTTTATCA TCCAAATGTT GTACGCTATT ATAAAACATT TCTGGAAAAT
    GATAGGCTGT ATATAGTTAT GGAACTTATA GAAGGGGCCC CTCTTGGAGA GCATTTCAGT
    TCTTTGAAGG AAAAACGACA TCATTTTGCT GAAGAGAGAC TATGGAAAAT ATTTATACAG
    CTGTGCTTGG CTCTTCGGTA CTTGCACAAG GAGAAGAGGA TCATCCATAG AGACCTGACG
    CCGAACAACA TCATGCTGGG GGACAAGGAC AGAGTGACCG TCACTGACTT TGGCCTGGCA
    AAGCAAAAAC AAGAGAGCAG TAGACTCACG TCTGTTGTGG GAACGATTCT GTACTCCTGC
    CCGGAGGTGC TGAAGAGCGA GCCGTATGGG GAAAAGGCAG ATGTGTGGGC AGCAGGCTGC
    ATCCTTTATC AGATGGCGAC CCTGGATCCT CCCTTCTACA GCACCAACAT GCTCTCCCTG
    GCCACAAAAA TAGTGGAGGC GGTATATGAA CCAGTGCCAG AAGGCATCTA CTCTGAAAAG
    GTGATCACTA CCATCAGCAG ATGCCTCACT CCAGATGCAG AAACCCGCCC AGATATTGTA
    GAAGTCAGCT CCATGATATC AGATATCATG ATGAAGTATG TAGACAATTT ATCCACATCC
    CAGCTGGCCT TGGAGAAGAA GCTGGAACGG GAACGGAGGC GCACACAGAG GTATTTTATG
    GAAGCCAACC GGAATGCAAT CATATGTCAG CAGGAGCCTA CTCTGCTGTC TCAGGAAACC
    TTTGAGAAGG CAAGCTTGAG TAGCAGCAGC AGTGGAGCAG GAAGCCTGAA AAGTGAACTT
    TCAGAAATCA CAGACCTCCC TGCTGAAGGC TTCCAGGCAC CATGTGTTAA GGATGAAGAC
    CGGACTTGTG ATGGACTCTT ATCAGATGAT AACTTCAATT TGGAAAATAT TGAGAAAGAT
    CTATATTCAG AGCTTGACGA TGAATTGGAC GTTTCGGATA ACTTTAGCAG CTCCGGCTCA
    AGCCCTTTGA AGGAATCGAC ATTCAGCACT TTAAAAAGAA GTTTTAGTGC TTCAGGAGGA
    GAAAGACAAT CCCAAACTAG AGACTTCACT GGTGGAATAG GATCCAGACT GAGACCAGCT
    TTGCTGCCTC TTGACCTGCT TCTGAAAGTG CCACCCCTCC TGCTCAGTGC CCACGTTAAG
    GAGGTAGAGG CTGAGTTAGT GACGGGGTGG CAGTCCCACA GCCTTCCCAC TGTGATTCTC
    CACAGTCTCA AACACCATGC ATCTGCAGGA ATTGCTGTGT CACAGAGGAA AGTACGTCAG
    ATCAGTGATC CTATTCAGCA GATCCTAATC CAGCTGCACA AAGTCATCTA CATCACTCAG
    CTTCCTCCAG CTTTGCACCA CAACTTGAAA AGAAGGATTA TAGAGAGATT CAAGAAATCC
    CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAA
    GGATCTCCAG AACCAATTGA ACCCAACTTT TTCACAGCAG ATTACCACTT ATTGCATCAT
    TCATTGGGTG GAAAGAGCCT GTCCTCAAAT GACCCAACAG GTCTACCCTC TAGCTTTGAT
    TTGGAGGAGG GGATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CTTAGAGGAA
    AGTGGCTATT ACAATTTTAC TTCTAACAGG TATCACTCCT ATCCATGGGG GACCAAGAAT
    TATCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.