ARFGEF3 cDNA ORF clone, Bos taurus(cattle)

The following ARFGEF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARFGEF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa22721 XM_024997029.1
Latest version!
Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa238091 XM_024997030.1
Latest version!
Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa91509 XM_015472886.1
Latest version!
Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa91509 XM_015464839.1
Latest version!
Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa22720 XM_002690274.5
Latest version!
Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa22720 XM_015464837.1
Latest version!
Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa22722 XM_005210993.4
Latest version!
Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa22722 XM_015464838.1
Latest version!
Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OBa22722 XM_005210993.3 Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa22720 XM_002690274.4 Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa22721
    Clone ID Related Accession (Same CDS sequence) XM_024997029.1
    Accession Version XM_024997029.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    GTGTGCATTG AAACATACAT ATGTAGCTGT CACCAGCGTA GTATAAACAC TGCTGTGCGG
    GCAACTCTCA GTCAAATGCT GAGTGACTTG ACTTTACAGT TACGACAAAG GCAGGAGAAC
    ACGATAATTG AAAACCCGGA TGTCCCCCAG GAATTCAGGA GTCAAGGGTC AACAGTAGAG
    TCCCTCTGTG ATGACCTTGT CTCCGTGCTC ACTGTTCTGT GTGAGAAGCT CCAAGCTGCC
    ATCAAGAAGA ACCTTTGCCC CGCTCTCATC GTGATCCTGG GGAATCCGAT TCATGATAAA
    ACCATCACCT CTGCACACAG CAGCAGCACG AGTACCAGCA TCGAGTCAGA CTCTGTGTCT
    CCTGGGGTTT CCGACCACGG CCGGGGTTCG GGCTGCTCCT CCACAGCGCC AGCCCTGAGT
    GGGCCAGTGG CCCGGACCAT CTACTACATC GCGGCTGAGC TGGTGCGGCT GGTGGGGTCG
    GTGGACTCCA TGAAGCCCGT GTTGCAGTCC CTCTACCACC GTGTGCTGCT CTACCCCCCA
    CCCCAGCACC GTGTGGAAGC CATCAAGATC ATGAAAGAGC TACTTGGGAG CCCAAAGCGT
    CTCTGTGATT TGGCAGGACC CAGCTCCACT GAACCTGACT CGAGAAAAAG ATCAATTTCA
    AAAAGAAAGT CTCATCTGGA TCTTCTCAAA CTCATCATGG ACGGCATGAC CGAGGCGTGT
    ATCAAGGGCG GCATCGAGGC CTGCTACGCC GCCGTGTCCT GCGTCTGCAC CTTGCTGGGA
    GCCTTGGAGG AGCTCAGCCA GGGCAGGGGC TTGAGCGAAA GTCAAGTCCA GCTGCTGCTG
    CTGCGCCTGG AGGAGCTGAA GGACGGGGCC GAGTCCAGCC GCGACTCCAT GGAGATCAAT
    GAGGCCGACT TCCGCTGGCA GCGGCGAGTC CTGTCCTCGG AACACACACC CTGGGAGTTG
    GGGAATGAGC GGAGCCTTGA CATCAGCATC AGCGTTACCA CGGACACGGG CCAGACCACC
    TTGGAGGGAG AGTTGGGTCA GACCACACCC GAGGACCACT CGGAAAACCC CAAGAATGGC
    CTCAAGTCAC CAGCCGTCCA GGAGGGCAAG GAGACTCTGA GTAAAGTGTC AGAACCAGAA
    GCTGTGGACC AGCCAGACGT GGTTCAAAGG AGCCACACGG TTGCCTACCC CGATATAACC
    AACTTCCTGT CCGTCGACTG CAGGATGAGG TCCTACGGAT CCAGATACAG TGAGAGCAAC
    TTCAGCGTGG ACGACCAAGA CCTCTCTCGG ACAGAATTCG ACTCCTGTGA CCAGTACTCC
    ATGGCGGCTG AAAAGGACTC CGGGAGATCG GACGTGTCGG ACATTGGATC CGACAACTGT
    TCTTTAGCTG ATGAGGAGCA GACCCCCCGG GACTGCCTGG GCCACAGGTC CCTGCGGACT
    GCAGCTCTGT CTCTCAAGCT GCTGAAGAAC CAGGAGGCTG ACCAGCACAG CGCCCGGCTG
    TTCGTGCAGT CGCTGGAGGG TCTCCTGCCG CGCCTCCTGG CCCTGCCCAG TGTGGAAGAG
    GTGGACTCAG CCCTCCAGAA CTTTGTCTCT ACCTTCTGCT CAGGTATGAT GCATTCACCA
    GGGTTTGATG GGAACAGCAG CCTGGGCCTG CAGATGCTGA TGAACGCGGA CAGCCTGTAC
    TCGGCGGCGC ACTGCGCGCT GCTGCTCAAC CTGAAGCTCT CCCACGGTGA CTACTACCGC
    AAGCGGCCCA CCCTAGCACC CAGCACCATG AAAGAGTTCA TGAAGCAGGT CCAGACCAGC
    GGGGTGCTGA TGGTCTTCTC CCAGGCCTGG ATTGAGGAAC TTTACCACCA GGTTCTTGAC
    AGAAACATGC TCGGAGAAGC CGGCTACTGG GGCAGCCCTG AAGACAACAG CCTCCCCCTC
    ATCACCATGC TGACGGATAT CGATGGCTTA GAGAGCAGTG CAATTGGAGG GCAGCTGATG
    GCCTCAGCTT CGATGGAGTC TCCTTTCACC CAGGGCAGGA GAATTGATGA CTCCACGGTG
    GCAGGTGTGG CATTTGCTCG CTATATTCTG ATTGGCTGCT GGAAGAACTT GATCGACACT
    TTATCAACCC CCCTGACGGG CCGAATGGCC GGCAGCTCCA AAGGGCTGGC CTTCATCTTG
    GGAGCTGAAG GCATCAAAGA ACAGAACCAA AAAGAACGCG ATGCTATCTG CATGAGCCTC
    GACGGGCTAC GGAAAGCCGC GCGTCTGAGC TGCGCTCTAG GAGTTGCAGC CAACTGTGCT
    TCGGCCCTCG CCCAGATGGC AGCTGCCTCC TGTGTCCAGG AGGAGAAAGA GGAAAAGGAA
    ACCCAGGAAC CCAGTGATGC CATAGCACAA GTGAAACTAA AAGTGGAGCA GAAGCTGGAG
    CAGATCGGGA AGCTGCAGGG GGTGTGGCTG CACACAGCCC ACGTCCTGTG CATGGACGCC
    ATCCTCAGTG TCGGCCTGGA GATGGGCAGC CACAACCCCG ACTGCTGGCC ACACGTGTTC
    AGGGTGTGCG AGTACGTGGG CACCTTGGAA CACACCCACT TCAGCGATGG CACCTCTCAG
    CCCCCGCTGA CCATCAGCCA GCCCCAGAAG GCCCCCAGAG GCGGACTCCT TGGGGACCTG
    GAGAGTGAGG GCTCAGCCCA GGAGCAGAGC CTGGAGCAGG AGAGCTCCCT GAGCGCGGCC
    CCCGTCATCC AACCGCTCTC CATCCAGGAG CTCGTCCGAG AAGGCAGCCG AGGCCGGGCC
    TCTGACTTCC GTGGGGGCAG CCTCATGACA GGCAGCAGTG CCGCCAAGGT GGTGCTCACG
    CTCTCCACGC AGGCTGACAG GCTCTTTGAA GATGCTACAG ATAAACTGAA CCTCATGGCT
    CTGGGAGGTT TTCTTTACCA GCTGAAGAAA GCATCGCAGT CCCAGCTTTT CCATTCTGTT
    ACGGAAACAG TTGATTACTC GCTGGCAATG CCAGGAGAAG TGAAGTCTAC CCAAGACCGC
    AAAAGTGCCC TCCACCTGTT CCGCCTCGGG GACGCCATGC TAAGGATCGT GAGGAGCAAA
    TCGAGGCCCC TGCTCCACGT GATGCGCTGC TGGAGCCTCG TGGCCCCACA CCTGGTAGAG
    GCTGCCTGCC ATAAGGAAAG ACACGTGTCT CAGAAGGCGG TTTCCTTCAT CCATGACATA
    CTGACGGAGG TTCTCACGGA CTGGAATGAG CCACCTCATT TCCACTTTAA CGAAGCTCTC
    TTCCGACCGT TCGAACGTAT CATGCAGCTG GAGTTGTGTG ACGAGGACGT CCAAGACCAG
    GTTGTCACGT CCATCGGGGA GCTGGTTGAA GTGTGCTCTG CCCAGATCCA GTCAGGCTGG
    AGGCCCCTGT TCAGCGCCCT GGAAACGGTG CACAGCGGGA ACAAGTCCGA GGTGAAGGAG
    TACCTGGTGG GCGACTATTC CATGGGAAAA GGCCAGGCTC CAGTGTTTGA TGTATTTGAA
    GCTTTTCTCA ATACCGACAA CATCCAGGTC TTTGCTAATG CAGCCACTAG TTACATCATG
    TGCCTTATGA AGTTTGTCAA AGGACTGGGG GAGGTGGACT GTAAGGAGAT TGGAGACTGT
    GTCCCAGGAC CCGGAGCCGC GTCCCCAGAC CTGTGCCTCC CCGCCCTGGA CTACCTCCGG
    CGCTGTTCTC AGTTGTTGGC CAAAATCTAC AAAATGCCCT TGAAGCCAAT ATTCCTTAGT
    GGGCGACTTG CTAGCTTGCC CCGAAGACTT CAGGAGCAGT CAGCGAGCAG TGAGGATGGA
    ATTGAGTCAG TCTTGTCTGA CTTTGATGAC GACACTGGTC TGATCGAAGT TTGGATCATC
    CTCTTGGAGC AGCTCACAGC AGCTGTGTCC AACTGTCCAC GTCAGCACCA ACCCCCCACT
    CTGGATTTGC TCTTTGAGCT GTTGAGAGAG GTTACCAAAA CACCTGGACC AGGGTTTGGC
    ATCTATGCAG TTGTTCACCT TCTCCTTCCT GTGATGTCTC TTTGGCTTCG CCGTAGCCAT
    AAAGACCATT CTTACTGGGA CATTGCCTCG GCTAACTTCA AGCATGCCAT TGGTCTGTCC
    TGTGAGCTGG TGGTGGAGCA CATTCAGAGC TTTATCCACT CAGACATCAG GTATGAGAGC
    ATGATCAACA CCATGCTGAA GGACCTCTTT GAGCTACTGG TAGCATGTGT AGCCAAGCCC
    ACAGAAACCA TCTCCCGAGT AGGCTGCTCC TGTATTAGGT ACGTCCTCGT GACAGCGGGC
    CCCGTGTTCA CGGAGGAGAT GTGGAGGCTG GCCTGCTGTG CCCTGCAGGA CGCCTTCTCG
    GCCACACTCA AGCCGGTGAA GGACCTGCTG GGCTGCTTCC ACAGTGGCAC CGAGAGCTTC
    AGCGGCGAAG GCTGCCAGGT GAGGGTGGCC GCCCCGTCCT CCTCCCCGAG TGCCGAGGCC
    GAGTACTGGC GCATCCGAGC CATGGCTCAG CAGGTATTTA TGTTGGATAC CCAGTGCTCA
    CCGAAGACTC CAAACAACTT CGATCATGCT CAGTCCTGTC AGCTCATTAT TGAACTGCCT
    CCTGATGAGA AGCCAAATGG GCATACCAAG AAAAGTGTTT CTTTCAGGGA AATTGTGGTG
    AGTTTACTAT CCCATCAAGT ATTACTCCAG AACTTATATG ATATCTTATT AGAAGAGTTT
    GTCAAAGGCC CCTCTCCTGG GGAGGAGAAG ATCCAAGGGC CAGAAACCAA GCTGGCTGGC
    TTCCTCAGAT ACATCTCCAT GCAGAACCTG GCCGTCATCT TTGACCTTCT GCTGGACTCC
    TACAGGACGG CCAGAGAGTT TGACACCAGC CCAGGGCTGA AGTGCCTGCT GAAGAAAGTG
    TCTGGCATAG GGGGCGCTGC CAACCTCTAC CGCCAGTCTG CCATGAGTTT CAACATTTAT
    TTCCACGCAC TGGTCTGTGC TGTTCTTAGC AACCAAGAAA CCATCACTGC TGACCAAGTG
    AAGAAGGTCC TTTTTGAGGA CGATGAGAGG AGCACAGATT CGTCCCAGCA GTGTTCATCA
    GAAGATGAAG ACATTTTTGA GGAGACTGCC CAGGTGAGCC CCCCGAGAGG CAAAGAAAAG
    AGGCAGTGGA GGGCTCGGAT GCCCTCGCTC AGCGTCCAGC CCGTCAGCAA CGCGGACTGG
    GTGTGGCTGG TCAAGAGGCT TCACAAGCTG TGCATGGAGC TGTGTAACAA CTACATCCAG
    ATGCACCTGG ACCTGGAAAA CAGTATGGAG GAGGCCCCAA TCTTCAAGAG TGACCCTTTC
    TTCATCCTTC CCTCCTTCCA GTCGGAGTCC TCCACCCCTT CCACCGGGGG CTTCTCAGGG
    AAAGACACGC CTTCCGAGGA TGACCGCAGT CAGACCCGGG ATCATGCGGG CGAGCCTCTG
    AGCCTGAGGG GGACCAGCGG GGAGCTGCTG CTGCTGCCCC CGAGCCCCAA GGTGGAGAAG
    AAGGATCCCA GCCGCAAGAA GGAGTGGTGG GAGAACGCGG GCAACAAAAT CTATACCATG
    GCAGCCGACA AGACCATCTC AAAGCTGATG ACCGAGTACA AGAGGAGGAA ACAGCAGCAC
    AACCTGCCTG TCTTCCCCAA AGAGATCAAG GTGGAGAAGA AGGGAGAGGT GCTGGGCGCC
    AGGGGCCAAG GCTCCCCACT GCTCCAGCGA CCCCAGCACC TGATGGACCA GGGGCAGATG
    CGGCACTCTT TCAGCGCCGG CCCTGAGCTG CTGCGCCAGG AGAAGAGGCC CCGCTCCGGC
    TCCACCGGCA GCTCCCGGAG TGTCTCCGCG AGGGACGCAG AAGCCCAGAT CCAGGCATGG
    ACCAACATGG TGCTAACAGT CCTCAATCAG ATTCAGATCC TCCCAGACCA GACCTTCACA
    GCCCTCCAGC CAGCAGTGTT TCCGTGTATC AGCCAGCTCA CCTGTCACGT GACTGACATC
    AGAGTTCGCC AGGCTGTGAG AGAATGGCTC GGCAGAGTGG GCCGTGTCTA TGACATCATT
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852797.1
    CDS94..6519
    Translation

    Target ORF information:

    RefSeq Version XM_024997029.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997029.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    GTGTGCATTG AAACATACAT ATGTAGCTGT CACCAGCGTA GTATAAACAC TGCTGTGCGG
    GCAACTCTCA GTCAAATGCT GAGTGACTTG ACTTTACAGT TACGACAAAG GCAGGAGAAC
    ACGATAATTG AAAACCCGGA TGTCCCCCAG GAATTCAGGA GTCAAGGGTC AACAGTAGAG
    TCCCTCTGTG ATGACCTTGT CTCCGTGCTC ACTGTTCTGT GTGAGAAGCT CCAAGCTGCC
    ATCAAGAAGA ACCTTTGCCC CGCTCTCATC GTGATCCTGG GGAATCCGAT TCATGATAAA
    ACCATCACCT CTGCACACAG CAGCAGCACG AGTACCAGCA TCGAGTCAGA CTCTGTGTCT
    CCTGGGGTTT CCGACCACGG CCGGGGTTCG GGCTGCTCCT CCACAGCGCC AGCCCTGAGT
    GGGCCAGTGG CCCGGACCAT CTACTACATC GCGGCTGAGC TGGTGCGGCT GGTGGGGTCG
    GTGGACTCCA TGAAGCCCGT GTTGCAGTCC CTCTACCACC GTGTGCTGCT CTACCCCCCA
    CCCCAGCACC GTGTGGAAGC CATCAAGATC ATGAAAGAGC TACTTGGGAG CCCAAAGCGT
    CTCTGTGATT TGGCAGGACC CAGCTCCACT GAACCTGACT CGAGAAAAAG ATCAATTTCA
    AAAAGAAAGT CTCATCTGGA TCTTCTCAAA CTCATCATGG ACGGCATGAC CGAGGCGTGT
    ATCAAGGGCG GCATCGAGGC CTGCTACGCC GCCGTGTCCT GCGTCTGCAC CTTGCTGGGA
    GCCTTGGAGG AGCTCAGCCA GGGCAGGGGC TTGAGCGAAA GTCAAGTCCA GCTGCTGCTG
    CTGCGCCTGG AGGAGCTGAA GGACGGGGCC GAGTCCAGCC GCGACTCCAT GGAGATCAAT
    GAGGCCGACT TCCGCTGGCA GCGGCGAGTC CTGTCCTCGG AACACACACC CTGGGAGTTG
    GGGAATGAGC GGAGCCTTGA CATCAGCATC AGCGTTACCA CGGACACGGG CCAGACCACC
    TTGGAGGGAG AGTTGGGTCA GACCACACCC GAGGACCACT CGGAAAACCC CAAGAATGGC
    CTCAAGTCAC CAGCCGTCCA GGAGGGCAAG GAGACTCTGA GTAAAGTGTC AGAACCAGAA
    GCTGTGGACC AGCCAGACGT GGTTCAAAGG AGCCACACGG TTGCCTACCC CGATATAACC
    AACTTCCTGT CCGTCGACTG CAGGATGAGG TCCTACGGAT CCAGATACAG TGAGAGCAAC
    TTCAGCGTGG ACGACCAAGA CCTCTCTCGG ACAGAATTCG ACTCCTGTGA CCAGTACTCC
    ATGGCGGCTG AAAAGGACTC CGGGAGATCG GACGTGTCGG ACATTGGATC CGACAACTGT
    TCTTTAGCTG ATGAGGAGCA GACCCCCCGG GACTGCCTGG GCCACAGGTC CCTGCGGACT
    GCAGCTCTGT CTCTCAAGCT GCTGAAGAAC CAGGAGGCTG ACCAGCACAG CGCCCGGCTG
    TTCGTGCAGT CGCTGGAGGG TCTCCTGCCG CGCCTCCTGG CCCTGCCCAG TGTGGAAGAG
    GTGGACTCAG CCCTCCAGAA CTTTGTCTCT ACCTTCTGCT CAGGTATGAT GCATTCACCA
    GGGTTTGATG GGAACAGCAG CCTGGGCCTG CAGATGCTGA TGAACGCGGA CAGCCTGTAC
    TCGGCGGCGC ACTGCGCGCT GCTGCTCAAC CTGAAGCTCT CCCACGGTGA CTACTACCGC
    AAGCGGCCCA CCCTAGCACC CAGCACCATG AAAGAGTTCA TGAAGCAGGT CCAGACCAGC
    GGGGTGCTGA TGGTCTTCTC CCAGGCCTGG ATTGAGGAAC TTTACCACCA GGTTCTTGAC
    AGAAACATGC TCGGAGAAGC CGGCTACTGG GGCAGCCCTG AAGACAACAG CCTCCCCCTC
    ATCACCATGC TGACGGATAT CGATGGCTTA GAGAGCAGTG CAATTGGAGG GCAGCTGATG
    GCCTCAGCTT CGATGGAGTC TCCTTTCACC CAGGGCAGGA GAATTGATGA CTCCACGGTG
    GCAGGTGTGG CATTTGCTCG CTATATTCTG ATTGGCTGCT GGAAGAACTT GATCGACACT
    TTATCAACCC CCCTGACGGG CCGAATGGCC GGCAGCTCCA AAGGGCTGGC CTTCATCTTG
    GGAGCTGAAG GCATCAAAGA ACAGAACCAA AAAGAACGCG ATGCTATCTG CATGAGCCTC
    GACGGGCTAC GGAAAGCCGC GCGTCTGAGC TGCGCTCTAG GAGTTGCAGC CAACTGTGCT
    TCGGCCCTCG CCCAGATGGC AGCTGCCTCC TGTGTCCAGG AGGAGAAAGA GGAAAAGGAA
    ACCCAGGAAC CCAGTGATGC CATAGCACAA GTGAAACTAA AAGTGGAGCA GAAGCTGGAG
    CAGATCGGGA AGCTGCAGGG GGTGTGGCTG CACACAGCCC ACGTCCTGTG CATGGACGCC
    ATCCTCAGTG TCGGCCTGGA GATGGGCAGC CACAACCCCG ACTGCTGGCC ACACGTGTTC
    AGGGTGTGCG AGTACGTGGG CACCTTGGAA CACACCCACT TCAGCGATGG CACCTCTCAG
    CCCCCGCTGA CCATCAGCCA GCCCCAGAAG GCCCCCAGAG GCGGACTCCT TGGGGACCTG
    GAGAGTGAGG GCTCAGCCCA GGAGCAGAGC CTGGAGCAGG AGAGCTCCCT GAGCGCGGCC
    CCCGTCATCC AACCGCTCTC CATCCAGGAG CTCGTCCGAG AAGGCAGCCG AGGCCGGGCC
    TCTGACTTCC GTGGGGGCAG CCTCATGACA GGCAGCAGTG CCGCCAAGGT GGTGCTCACG
    CTCTCCACGC AGGCTGACAG GCTCTTTGAA GATGCTACAG ATAAACTGAA CCTCATGGCT
    CTGGGAGGTT TTCTTTACCA GCTGAAGAAA GCATCGCAGT CCCAGCTTTT CCATTCTGTT
    ACGGAAACAG TTGATTACTC GCTGGCAATG CCAGGAGAAG TGAAGTCTAC CCAAGACCGC
    AAAAGTGCCC TCCACCTGTT CCGCCTCGGG GACGCCATGC TAAGGATCGT GAGGAGCAAA
    TCGAGGCCCC TGCTCCACGT GATGCGCTGC TGGAGCCTCG TGGCCCCACA CCTGGTAGAG
    GCTGCCTGCC ATAAGGAAAG ACACGTGTCT CAGAAGGCGG TTTCCTTCAT CCATGACATA
    CTGACGGAGG TTCTCACGGA CTGGAATGAG CCACCTCATT TCCACTTTAA CGAAGCTCTC
    TTCCGACCGT TCGAACGTAT CATGCAGCTG GAGTTGTGTG ACGAGGACGT CCAAGACCAG
    GTTGTCACGT CCATCGGGGA GCTGGTTGAA GTGTGCTCTG CCCAGATCCA GTCAGGCTGG
    AGGCCCCTGT TCAGCGCCCT GGAAACGGTG CACAGCGGGA ACAAGTCCGA GGTGAAGGAG
    TACCTGGTGG GCGACTATTC CATGGGAAAA GGCCAGGCTC CAGTGTTTGA TGTATTTGAA
    GCTTTTCTCA ATACCGACAA CATCCAGGTC TTTGCTAATG CAGCCACTAG TTACATCATG
    TGCCTTATGA AGTTTGTCAA AGGACTGGGG GAGGTGGACT GTAAGGAGAT TGGAGACTGT
    GTCCCAGGAC CCGGAGCCGC GTCCCCAGAC CTGTGCCTCC CCGCCCTGGA CTACCTCCGG
    CGCTGTTCTC AGTTGTTGGC CAAAATCTAC AAAATGCCCT TGAAGCCAAT ATTCCTTAGT
    GGGCGACTTG CTAGCTTGCC CCGAAGACTT CAGGAGCAGT CAGCGAGCAG TGAGGATGGA
    ATTGAGTCAG TCTTGTCTGA CTTTGATGAC GACACTGGTC TGATCGAAGT TTGGATCATC
    CTCTTGGAGC AGCTCACAGC AGCTGTGTCC AACTGTCCAC GTCAGCACCA ACCCCCCACT
    CTGGATTTGC TCTTTGAGCT GTTGAGAGAG GTTACCAAAA CACCTGGACC AGGGTTTGGC
    ATCTATGCAG TTGTTCACCT TCTCCTTCCT GTGATGTCTC TTTGGCTTCG CCGTAGCCAT
    AAAGACCATT CTTACTGGGA CATTGCCTCG GCTAACTTCA AGCATGCCAT TGGTCTGTCC
    TGTGAGCTGG TGGTGGAGCA CATTCAGAGC TTTATCCACT CAGACATCAG GTATGAGAGC
    ATGATCAACA CCATGCTGAA GGACCTCTTT GAGCTACTGG TAGCATGTGT AGCCAAGCCC
    ACAGAAACCA TCTCCCGAGT AGGCTGCTCC TGTATTAGGT ACGTCCTCGT GACAGCGGGC
    CCCGTGTTCA CGGAGGAGAT GTGGAGGCTG GCCTGCTGTG CCCTGCAGGA CGCCTTCTCG
    GCCACACTCA AGCCGGTGAA GGACCTGCTG GGCTGCTTCC ACAGTGGCAC CGAGAGCTTC
    AGCGGCGAAG GCTGCCAGGT GAGGGTGGCC GCCCCGTCCT CCTCCCCGAG TGCCGAGGCC
    GAGTACTGGC GCATCCGAGC CATGGCTCAG CAGGTATTTA TGTTGGATAC CCAGTGCTCA
    CCGAAGACTC CAAACAACTT CGATCATGCT CAGTCCTGTC AGCTCATTAT TGAACTGCCT
    CCTGATGAGA AGCCAAATGG GCATACCAAG AAAAGTGTTT CTTTCAGGGA AATTGTGGTG
    AGTTTACTAT CCCATCAAGT ATTACTCCAG AACTTATATG ATATCTTATT AGAAGAGTTT
    GTCAAAGGCC CCTCTCCTGG GGAGGAGAAG ATCCAAGGGC CAGAAACCAA GCTGGCTGGC
    TTCCTCAGAT ACATCTCCAT GCAGAACCTG GCCGTCATCT TTGACCTTCT GCTGGACTCC
    TACAGGACGG CCAGAGAGTT TGACACCAGC CCAGGGCTGA AGTGCCTGCT GAAGAAAGTG
    TCTGGCATAG GGGGCGCTGC CAACCTCTAC CGCCAGTCTG CCATGAGTTT CAACATTTAT
    TTCCACGCAC TGGTCTGTGC TGTTCTTAGC AACCAAGAAA CCATCACTGC TGACCAAGTG
    AAGAAGGTCC TTTTTGAGGA CGATGAGAGG AGCACAGATT CGTCCCAGCA GTGTTCATCA
    GAAGATGAAG ACATTTTTGA GGAGACTGCC CAGGTGAGCC CCCCGAGAGG CAAAGAAAAG
    AGGCAGTGGA GGGCTCGGAT GCCCTCGCTC AGCGTCCAGC CCGTCAGCAA CGCGGACTGG
    GTGTGGCTGG TCAAGAGGCT TCACAAGCTG TGCATGGAGC TGTGTAACAA CTACATCCAG
    ATGCACCTGG ACCTGGAAAA CAGTATGGAG GAGGCCCCAA TCTTCAAGAG TGACCCTTTC
    TTCATCCTTC CCTCCTTCCA GTCGGAGTCC TCCACCCCTT CCACCGGGGG CTTCTCAGGG
    AAAGACACGC CTTCCGAGGA TGACCGCAGT CAGACCCGGG ATCATGCGGG CGAGCCTCTG
    AGCCTGAGGG GGACCAGCGG GGAGCTGCTG CTGCTGCCCC CGAGCCCCAA GGTGGAGAAG
    AAGGATCCCA GCCGCAAGAA GGAGTGGTGG GAGAACGCGG GCAACAAAAT CTATACCATG
    GCAGCCGACA AGACCATCTC AAAGCTGATG ACCGAGTACA AGAGGAGGAA ACAGCAGCAC
    AACCTGCCTG TCTTCCCCAA AGAGATCAAG GTGGAGAAGA AGGGAGAGGT GCTGGGCGCC
    AGGGGCCAAG GCTCCCCACT GCTCCAGCGA CCCCAGCACC TGATGGACCA GGGGCAGATG
    CGGCACTCTT TCAGCGCCGG CCCTGAGCTG CTGCGCCAGG AGAAGAGGCC CCGCTCCGGC
    TCCACCGGCA GCTCCCGGAG TGTCTCCGCG AGGGACGCAG AAGCCCAGAT CCAGGCATGG
    ACCAACATGG TGCTAACAGT CCTCAATCAG ATTCAGATCC TCCCAGACCA GACCTTCACA
    GCCCTCCAGC CAGCAGTGTT TCCGTGTATC AGCCAGCTCA CCTGTCACGT GACTGACATC
    AGAGTTCGCC AGGCTGTGAG AGAATGGCTC GGCAGAGTGG GCCGTGTCTA TGACATCATT
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238091
    Clone ID Related Accession (Same CDS sequence) XM_024997030.1
    Accession Version XM_024997030.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6303bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    ATAATTGAAA ACCCGGATGT CCCCCAGGAA TTCAGGAGTC AAGGGTCAAC AGTAGAGTCC
    CTCTGTGATG ACCTTGTCTC CGTGCTCACT GTTCTGTGTG AGAAGCTCCA AGCTGCCATC
    AAGAAGAACC TTTGCCCCGC TCTCATCGTG ATCCTGGGGA ATCCGATTCA TGATAAAACC
    ATCACCTCTG CACACAGCAG CAGCACGAGT ACCAGCATCG AGTCAGACTC TGTGTCTCCT
    GGGGTTTCCG ACCACGGCCG GGGTTCGGGC TGCTCCTCCA CAGCGCCAGC CCTGAGTGGG
    CCAGTGGCCC GGACCATCTA CTACATCGCG GCTGAGCTGG TGCGGCTGGT GGGGTCGGTG
    GACTCCATGA AGCCCGTGTT GCAGTCCCTC TACCACCGTG TGCTGCTCTA CCCCCCACCC
    CAGCACCGTG TGGAAGCCAT CAAGATCATG AAAGAGCTAC TTGGGAGCCC AAAGCGTCTC
    TGTGATTTGG CAGGACCCAG CTCCACTGAA CCTGACTCGA GAAAAAGATC AATTTCAAAA
    AGAAAGTCTC ATCTGGATCT TCTCAAACTC ATCATGGACG GCATGACCGA GGCGTGTATC
    AAGGGCGGCA TCGAGGCCTG CTACGCCGCC GTGTCCTGCG TCTGCACCTT GCTGGGAGCC
    TTGGAGGAGC TCAGCCAGGG CAGGGGCTTG AGCGAAAGTC AAGTCCAGCT GCTGCTGCTG
    CGCCTGGAGG AGCTGAAGGA CGGGGCCGAG TCCAGCCGCG ACTCCATGGA GATCAATGAG
    GCCGACTTCC GCTGGCAGCG GCGAGTCCTG TCCTCGGAAC ACACACCCTG GGAGTTGGGG
    AATGAGCGGA GCCTTGACAT CAGCATCAGC GTTACCACGG ACACGGGCCA GACCACCTTG
    GAGGGAGAGT TGGGTCAGAC CACACCCGAG GACCACTCGG AAAACCCCAA GAATGGCCTC
    AAGTCACCAG CCGTCCAGGA GGGCAAGGAG ACTCTGAGTA AAGTGTCAGA ACCAGAAGCT
    GTGGACCAGC CAGACGTGGT TCAAAGGAGC CACACGGTTG CCTACCCCGA TATAACCAAC
    TTCCTGTCCG TCGACTGCAG GATGAGGTCC TACGGATCCA GATACAGTGA GAGCAACTTC
    AGCGTGGACG ACCAAGACCT CTCTCGGACA GAATTCGACT CCTGTGACCA GTACTCCATG
    GCGGCTGAAA AGGACTCCGG GAGATCGGAC GTGTCGGACA TTGGATCCGA CAACTGTTCT
    TTAGCTGATG AGGAGCAGAC CCCCCGGGAC TGCCTGGGCC ACAGGTCCCT GCGGACTGCA
    GCTCTGTCTC TCAAGCTGCT GAAGAACCAG GAGGCTGACC AGCACAGCGC CCGGCTGTTC
    GTGCAGTCGC TGGAGGGTCT CCTGCCGCGC CTCCTGGCCC TGCCCAGTGT GGAAGAGGTG
    GACTCAGCCC TCCAGAACTT TGTCTCTACC TTCTGCTCAG GTATGATGCA TTCACCAGGG
    TTTGATGGGA ACAGCAGCCT GGGCCTGCAG ATGCTGATGA ACGCGGACAG CCTGTACTCG
    GCGGCGCACT GCGCGCTGCT GCTCAACCTG AAGCTCTCCC ACGGTGACTA CTACCGCAAG
    CGGCCCACCC TAGCACCCAG CACCATGAAA GAGTTCATGA AGCAGGTCCA GACCAGCGGG
    GTGCTGATGG TCTTCTCCCA GGCCTGGATT GAGGAACTTT ACCACCAGGT TCTTGACAGA
    AACATGCTCG GAGAAGCCGG CTACTGGGGC AGCCCTGAAG ACAACAGCCT CCCCCTCATC
    ACCATGCTGA CGGATATCGA TGGCTTAGAG AGCAGTGCAA TTGGAGGGCA GCTGATGGCC
    TCAGCTTCGA TGGAGTCTCC TTTCACCCAG GGCAGGAGAA TTGATGACTC CACGGTGGCA
    GGTGTGGCAT TTGCTCGCTA TATTCTGATT GGCTGCTGGA AGAACTTGAT CGACACTTTA
    TCAACCCCCC TGACGGGCCG AATGGCCGGC AGCTCCAAAG GGCTGGCCTT CATCTTGGGA
    GCTGAAGGCA TCAAAGAACA GAACCAAAAA GAACGCGATG CTATCTGCAT GAGCCTCGAC
    GGGCTACGGA AAGCCGCGCG TCTGAGCTGC GCTCTAGGAG TTGCAGCCAA CTGTGCTTCG
    GCCCTCGCCC AGATGGCAGC TGCCTCCTGT GTCCAGGAGG AGAAAGAGGA AAAGGAAACC
    CAGGAACCCA GTGATGCCAT AGCACAAGTG AAACTAAAAG TGGAGCAGAA GCTGGAGCAG
    ATCGGGAAGC TGCAGGGGGT GTGGCTGCAC ACAGCCCACG TCCTGTGCAT GGACGCCATC
    CTCAGTGTCG GCCTGGAGAT GGGCAGCCAC AACCCCGACT GCTGGCCACA CGTGTTCAGG
    GTGTGCGAGT ACGTGGGCAC CTTGGAACAC ACCCACTTCA GCGATGGCAC CTCTCAGCCC
    CCGCTGACCA TCAGCCAGCC CCAGAAGGCC CCCAGAGGCG GACTCCTTGG GGACCTGGAG
    AGTGAGGGCT CAGCCCAGGA GCAGAGCCTG GAGCAGGAGA GCTCCCTGAG CGCGGCCCCC
    GTCATCCAAC CGCTCTCCAT CCAGGAGCTC GTCCGAGAAG GCAGCCGAGG CCGGGCCTCT
    GACTTCCGTG GGGGCAGCCT CATGACAGGC AGCAGTGCCG CCAAGGTGGT GCTCACGCTC
    TCCACGCAGG CTGACAGGCT CTTTGAAGAT GCTACAGATA AACTGAACCT CATGGCTCTG
    GGAGGTTTTC TTTACCAGCT GAAGAAAGCA TCGCAGTCCC AGCTTTTCCA TTCTGTTACG
    GAAACAGTTG ATTACTCGCT GGCAATGCCA GGAGAAGTGA AGTCTACCCA AGACCGCAAA
    AGTGCCCTCC ACCTGTTCCG CCTCGGGGAC GCCATGCTAA GGATCGTGAG GAGCAAATCG
    AGGCCCCTGC TCCACGTGAT GCGCTGCTGG AGCCTCGTGG CCCCACACCT GGTAGAGGCT
    GCCTGCCATA AGGAAAGACA CGTGTCTCAG AAGGCGGTTT CCTTCATCCA TGACATACTG
    ACGGAGGTTC TCACGGACTG GAATGAGCCA CCTCATTTCC ACTTTAACGA AGCTCTCTTC
    CGACCGTTCG AACGTATCAT GCAGCTGGAG TTGTGTGACG AGGACGTCCA AGACCAGGTT
    GTCACGTCCA TCGGGGAGCT GGTTGAAGTG TGCTCTGCCC AGATCCAGTC AGGCTGGAGG
    CCCCTGTTCA GCGCCCTGGA AACGGTGCAC AGCGGGAACA AGTCCGAGGT GAAGGAGTAC
    CTGGTGGGCG ACTATTCCAT GGGAAAAGGC CAGGCTCCAG TGTTTGATGT ATTTGAAGCT
    TTTCTCAATA CCGACAACAT CCAGGTCTTT GCTAATGCAG CCACTAGTTA CATCATGTGC
    CTTATGAAGT TTGTCAAAGG ACTGGGGGAG GTGGACTGTA AGGAGATTGG AGACTGTGTC
    CCAGGACCCG GAGCCGCGTC CCCAGACCTG TGCCTCCCCG CCCTGGACTA CCTCCGGCGC
    TGTTCTCAGT TGTTGGCCAA AATCTACAAA ATGCCCTTGA AGCCAATATT CCTTAGTGGG
    CGACTTGCTA GCTTGCCCCG AAGACTTCAG GAGCAGTCAG CGAGCAGTGA GGATGGAATT
    GAGTCAGTCT TGTCTGACTT TGATGACGAC ACTGGTCTGA TCGAAGTTTG GATCATCCTC
    TTGGAGCAGC TCACAGCAGC TGTGTCCAAC TGTCCACGTC AGCACCAACC CCCCACTCTG
    GATTTGCTCT TTGAGCTGTT GAGAGAGGTT ACCAAAACAC CTGGACCAGG GTTTGGCATC
    TATGCAGTTG TTCACCTTCT CCTTCCTGTG ATGTCTCTTT GGCTTCGCCG TAGCCATAAA
    GACCATTCTT ACTGGGACAT TGCCTCGGCT AACTTCAAGC ATGCCATTGG TCTGTCCTGT
    GAGCTGGTGG TGGAGCACAT TCAGAGCTTT ATCCACTCAG ACATCAGGTA TGAGAGCATG
    ATCAACACCA TGCTGAAGGA CCTCTTTGAG CTACTGGTAG CATGTGTAGC CAAGCCCACA
    GAAACCATCT CCCGAGTAGG CTGCTCCTGT ATTAGGTACG TCCTCGTGAC AGCGGGCCCC
    GTGTTCACGG AGGAGATGTG GAGGCTGGCC TGCTGTGCCC TGCAGGACGC CTTCTCGGCC
    ACACTCAAGC CGGTGAAGGA CCTGCTGGGC TGCTTCCACA GTGGCACCGA GAGCTTCAGC
    GGCGAAGGCT GCCAGGTGAG GGTGGCCGCC CCGTCCTCCT CCCCGAGTGC CGAGGCCGAG
    TACTGGCGCA TCCGAGCCAT GGCTCAGCAG GTATTTATGT TGGATACCCA GTGCTCACCG
    AAGACTCCAA ACAACTTCGA TCATGCTCAG TCCTGTCAGC TCATTATTGA ACTGCCTCCT
    GATGAGAAGC CAAATGGGCA TACCAAGAAA AGTGTTTCTT TCAGGGAAAT TGTGGTGAGT
    TTACTATCCC ATCAAGTATT ACTCCAGAAC TTATATGATA TCTTATTAGA AGAGTTTGTC
    AAAGGCCCCT CTCCTGGGGA GGAGAAGATC CAAGGGCCAG AAACCAAGCT GGCTGGCTTC
    CTCAGATACA TCTCCATGCA GAACCTGGCC GTCATCTTTG ACCTTCTGCT GGACTCCTAC
    AGGACGGCCA GAGAGTTTGA CACCAGCCCA GGGCTGAAGT GCCTGCTGAA GAAAGTGTCT
    GGCATAGGGG GCGCTGCCAA CCTCTACCGC CAGTCTGCCA TGAGTTTCAA CATTTATTTC
    CACGCACTGG TCTGTGCTGT TCTTAGCAAC CAAGAAACCA TCACTGCTGA CCAAGTGAAG
    AAGGTCCTTT TTGAGGACGA TGAGAGGAGC ACAGATTCGT CCCAGCAGTG TTCATCAGAA
    GATGAAGACA TTTTTGAGGA GACTGCCCAG GTGAGCCCCC CGAGAGGCAA AGAAAAGAGG
    CAGTGGAGGG CTCGGATGCC CTCGCTCAGC GTCCAGCCCG TCAGCAACGC GGACTGGGTG
    TGGCTGGTCA AGAGGCTTCA CAAGCTGTGC ATGGAGCTGT GTAACAACTA CATCCAGATG
    CACCTGGACC TGGAAAACAG TATGGAGGAG GCCCCAATCT TCAAGAGTGA CCCTTTCTTC
    ATCCTTCCCT CCTTCCAGTC GGAGTCCTCC ACCCCTTCCA CCGGGGGCTT CTCAGGGAAA
    GACACGCCTT CCGAGGATGA CCGCAGTCAG ACCCGGGATC ATGCGGGCGA GCCTCTGAGC
    CTGAGGGGGA CCAGCGGGGA GCTGCTGCTG CTGCCCCCGA GCCCCAAGGT GGAGAAGAAG
    GATCCCAGCC GCAAGAAGGA GTGGTGGGAG AACGCGGGCA ACAAAATCTA TACCATGGCA
    GCCGACAAGA CCATCTCAAA GCTGATGACC GAGTACAAGA GGAGGAAACA GCAGCACAAC
    CTGCCTGTCT TCCCCAAAGA GATCAAGGTG GAGAAGAAGG GAGAGGTGCT GGGCGCCAGG
    GGCCAAGGCT CCCCACTGCT CCAGCGACCC CAGCACCTGA TGGACCAGGG GCAGATGCGG
    CACTCTTTCA GCGCCGGCCC TGAGCTGCTG CGCCAGGAGA AGAGGCCCCG CTCCGGCTCC
    ACCGGCAGCT CCCGGAGTGT CTCCGCGAGG GACGCAGAAG CCCAGATCCA GGCATGGACC
    AACATGGTGC TAACAGTCCT CAATCAGATT CAGATCCTCC CAGACCAGAC CTTCACAGCC
    CTCCAGCCAG CAGTGTTTCC GTGTATCAGC CAGCTCACCT GTCACGTGAC TGACATCAGA
    GTTCGCCAGG CTGTGAGAGA ATGGCTCGGC AGAGTGGGCC GTGTCTATGA CATCATTATG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852798.1
    CDS94..6396
    Translation

    Target ORF information:

    RefSeq Version XM_024997030.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    ATAATTGAAA ACCCGGATGT CCCCCAGGAA TTCAGGAGTC AAGGGTCAAC AGTAGAGTCC
    CTCTGTGATG ACCTTGTCTC CGTGCTCACT GTTCTGTGTG AGAAGCTCCA AGCTGCCATC
    AAGAAGAACC TTTGCCCCGC TCTCATCGTG ATCCTGGGGA ATCCGATTCA TGATAAAACC
    ATCACCTCTG CACACAGCAG CAGCACGAGT ACCAGCATCG AGTCAGACTC TGTGTCTCCT
    GGGGTTTCCG ACCACGGCCG GGGTTCGGGC TGCTCCTCCA CAGCGCCAGC CCTGAGTGGG
    CCAGTGGCCC GGACCATCTA CTACATCGCG GCTGAGCTGG TGCGGCTGGT GGGGTCGGTG
    GACTCCATGA AGCCCGTGTT GCAGTCCCTC TACCACCGTG TGCTGCTCTA CCCCCCACCC
    CAGCACCGTG TGGAAGCCAT CAAGATCATG AAAGAGCTAC TTGGGAGCCC AAAGCGTCTC
    TGTGATTTGG CAGGACCCAG CTCCACTGAA CCTGACTCGA GAAAAAGATC AATTTCAAAA
    AGAAAGTCTC ATCTGGATCT TCTCAAACTC ATCATGGACG GCATGACCGA GGCGTGTATC
    AAGGGCGGCA TCGAGGCCTG CTACGCCGCC GTGTCCTGCG TCTGCACCTT GCTGGGAGCC
    TTGGAGGAGC TCAGCCAGGG CAGGGGCTTG AGCGAAAGTC AAGTCCAGCT GCTGCTGCTG
    CGCCTGGAGG AGCTGAAGGA CGGGGCCGAG TCCAGCCGCG ACTCCATGGA GATCAATGAG
    GCCGACTTCC GCTGGCAGCG GCGAGTCCTG TCCTCGGAAC ACACACCCTG GGAGTTGGGG
    AATGAGCGGA GCCTTGACAT CAGCATCAGC GTTACCACGG ACACGGGCCA GACCACCTTG
    GAGGGAGAGT TGGGTCAGAC CACACCCGAG GACCACTCGG AAAACCCCAA GAATGGCCTC
    AAGTCACCAG CCGTCCAGGA GGGCAAGGAG ACTCTGAGTA AAGTGTCAGA ACCAGAAGCT
    GTGGACCAGC CAGACGTGGT TCAAAGGAGC CACACGGTTG CCTACCCCGA TATAACCAAC
    TTCCTGTCCG TCGACTGCAG GATGAGGTCC TACGGATCCA GATACAGTGA GAGCAACTTC
    AGCGTGGACG ACCAAGACCT CTCTCGGACA GAATTCGACT CCTGTGACCA GTACTCCATG
    GCGGCTGAAA AGGACTCCGG GAGATCGGAC GTGTCGGACA TTGGATCCGA CAACTGTTCT
    TTAGCTGATG AGGAGCAGAC CCCCCGGGAC TGCCTGGGCC ACAGGTCCCT GCGGACTGCA
    GCTCTGTCTC TCAAGCTGCT GAAGAACCAG GAGGCTGACC AGCACAGCGC CCGGCTGTTC
    GTGCAGTCGC TGGAGGGTCT CCTGCCGCGC CTCCTGGCCC TGCCCAGTGT GGAAGAGGTG
    GACTCAGCCC TCCAGAACTT TGTCTCTACC TTCTGCTCAG GTATGATGCA TTCACCAGGG
    TTTGATGGGA ACAGCAGCCT GGGCCTGCAG ATGCTGATGA ACGCGGACAG CCTGTACTCG
    GCGGCGCACT GCGCGCTGCT GCTCAACCTG AAGCTCTCCC ACGGTGACTA CTACCGCAAG
    CGGCCCACCC TAGCACCCAG CACCATGAAA GAGTTCATGA AGCAGGTCCA GACCAGCGGG
    GTGCTGATGG TCTTCTCCCA GGCCTGGATT GAGGAACTTT ACCACCAGGT TCTTGACAGA
    AACATGCTCG GAGAAGCCGG CTACTGGGGC AGCCCTGAAG ACAACAGCCT CCCCCTCATC
    ACCATGCTGA CGGATATCGA TGGCTTAGAG AGCAGTGCAA TTGGAGGGCA GCTGATGGCC
    TCAGCTTCGA TGGAGTCTCC TTTCACCCAG GGCAGGAGAA TTGATGACTC CACGGTGGCA
    GGTGTGGCAT TTGCTCGCTA TATTCTGATT GGCTGCTGGA AGAACTTGAT CGACACTTTA
    TCAACCCCCC TGACGGGCCG AATGGCCGGC AGCTCCAAAG GGCTGGCCTT CATCTTGGGA
    GCTGAAGGCA TCAAAGAACA GAACCAAAAA GAACGCGATG CTATCTGCAT GAGCCTCGAC
    GGGCTACGGA AAGCCGCGCG TCTGAGCTGC GCTCTAGGAG TTGCAGCCAA CTGTGCTTCG
    GCCCTCGCCC AGATGGCAGC TGCCTCCTGT GTCCAGGAGG AGAAAGAGGA AAAGGAAACC
    CAGGAACCCA GTGATGCCAT AGCACAAGTG AAACTAAAAG TGGAGCAGAA GCTGGAGCAG
    ATCGGGAAGC TGCAGGGGGT GTGGCTGCAC ACAGCCCACG TCCTGTGCAT GGACGCCATC
    CTCAGTGTCG GCCTGGAGAT GGGCAGCCAC AACCCCGACT GCTGGCCACA CGTGTTCAGG
    GTGTGCGAGT ACGTGGGCAC CTTGGAACAC ACCCACTTCA GCGATGGCAC CTCTCAGCCC
    CCGCTGACCA TCAGCCAGCC CCAGAAGGCC CCCAGAGGCG GACTCCTTGG GGACCTGGAG
    AGTGAGGGCT CAGCCCAGGA GCAGAGCCTG GAGCAGGAGA GCTCCCTGAG CGCGGCCCCC
    GTCATCCAAC CGCTCTCCAT CCAGGAGCTC GTCCGAGAAG GCAGCCGAGG CCGGGCCTCT
    GACTTCCGTG GGGGCAGCCT CATGACAGGC AGCAGTGCCG CCAAGGTGGT GCTCACGCTC
    TCCACGCAGG CTGACAGGCT CTTTGAAGAT GCTACAGATA AACTGAACCT CATGGCTCTG
    GGAGGTTTTC TTTACCAGCT GAAGAAAGCA TCGCAGTCCC AGCTTTTCCA TTCTGTTACG
    GAAACAGTTG ATTACTCGCT GGCAATGCCA GGAGAAGTGA AGTCTACCCA AGACCGCAAA
    AGTGCCCTCC ACCTGTTCCG CCTCGGGGAC GCCATGCTAA GGATCGTGAG GAGCAAATCG
    AGGCCCCTGC TCCACGTGAT GCGCTGCTGG AGCCTCGTGG CCCCACACCT GGTAGAGGCT
    GCCTGCCATA AGGAAAGACA CGTGTCTCAG AAGGCGGTTT CCTTCATCCA TGACATACTG
    ACGGAGGTTC TCACGGACTG GAATGAGCCA CCTCATTTCC ACTTTAACGA AGCTCTCTTC
    CGACCGTTCG AACGTATCAT GCAGCTGGAG TTGTGTGACG AGGACGTCCA AGACCAGGTT
    GTCACGTCCA TCGGGGAGCT GGTTGAAGTG TGCTCTGCCC AGATCCAGTC AGGCTGGAGG
    CCCCTGTTCA GCGCCCTGGA AACGGTGCAC AGCGGGAACA AGTCCGAGGT GAAGGAGTAC
    CTGGTGGGCG ACTATTCCAT GGGAAAAGGC CAGGCTCCAG TGTTTGATGT ATTTGAAGCT
    TTTCTCAATA CCGACAACAT CCAGGTCTTT GCTAATGCAG CCACTAGTTA CATCATGTGC
    CTTATGAAGT TTGTCAAAGG ACTGGGGGAG GTGGACTGTA AGGAGATTGG AGACTGTGTC
    CCAGGACCCG GAGCCGCGTC CCCAGACCTG TGCCTCCCCG CCCTGGACTA CCTCCGGCGC
    TGTTCTCAGT TGTTGGCCAA AATCTACAAA ATGCCCTTGA AGCCAATATT CCTTAGTGGG
    CGACTTGCTA GCTTGCCCCG AAGACTTCAG GAGCAGTCAG CGAGCAGTGA GGATGGAATT
    GAGTCAGTCT TGTCTGACTT TGATGACGAC ACTGGTCTGA TCGAAGTTTG GATCATCCTC
    TTGGAGCAGC TCACAGCAGC TGTGTCCAAC TGTCCACGTC AGCACCAACC CCCCACTCTG
    GATTTGCTCT TTGAGCTGTT GAGAGAGGTT ACCAAAACAC CTGGACCAGG GTTTGGCATC
    TATGCAGTTG TTCACCTTCT CCTTCCTGTG ATGTCTCTTT GGCTTCGCCG TAGCCATAAA
    GACCATTCTT ACTGGGACAT TGCCTCGGCT AACTTCAAGC ATGCCATTGG TCTGTCCTGT
    GAGCTGGTGG TGGAGCACAT TCAGAGCTTT ATCCACTCAG ACATCAGGTA TGAGAGCATG
    ATCAACACCA TGCTGAAGGA CCTCTTTGAG CTACTGGTAG CATGTGTAGC CAAGCCCACA
    GAAACCATCT CCCGAGTAGG CTGCTCCTGT ATTAGGTACG TCCTCGTGAC AGCGGGCCCC
    GTGTTCACGG AGGAGATGTG GAGGCTGGCC TGCTGTGCCC TGCAGGACGC CTTCTCGGCC
    ACACTCAAGC CGGTGAAGGA CCTGCTGGGC TGCTTCCACA GTGGCACCGA GAGCTTCAGC
    GGCGAAGGCT GCCAGGTGAG GGTGGCCGCC CCGTCCTCCT CCCCGAGTGC CGAGGCCGAG
    TACTGGCGCA TCCGAGCCAT GGCTCAGCAG GTATTTATGT TGGATACCCA GTGCTCACCG
    AAGACTCCAA ACAACTTCGA TCATGCTCAG TCCTGTCAGC TCATTATTGA ACTGCCTCCT
    GATGAGAAGC CAAATGGGCA TACCAAGAAA AGTGTTTCTT TCAGGGAAAT TGTGGTGAGT
    TTACTATCCC ATCAAGTATT ACTCCAGAAC TTATATGATA TCTTATTAGA AGAGTTTGTC
    AAAGGCCCCT CTCCTGGGGA GGAGAAGATC CAAGGGCCAG AAACCAAGCT GGCTGGCTTC
    CTCAGATACA TCTCCATGCA GAACCTGGCC GTCATCTTTG ACCTTCTGCT GGACTCCTAC
    AGGACGGCCA GAGAGTTTGA CACCAGCCCA GGGCTGAAGT GCCTGCTGAA GAAAGTGTCT
    GGCATAGGGG GCGCTGCCAA CCTCTACCGC CAGTCTGCCA TGAGTTTCAA CATTTATTTC
    CACGCACTGG TCTGTGCTGT TCTTAGCAAC CAAGAAACCA TCACTGCTGA CCAAGTGAAG
    AAGGTCCTTT TTGAGGACGA TGAGAGGAGC ACAGATTCGT CCCAGCAGTG TTCATCAGAA
    GATGAAGACA TTTTTGAGGA GACTGCCCAG GTGAGCCCCC CGAGAGGCAA AGAAAAGAGG
    CAGTGGAGGG CTCGGATGCC CTCGCTCAGC GTCCAGCCCG TCAGCAACGC GGACTGGGTG
    TGGCTGGTCA AGAGGCTTCA CAAGCTGTGC ATGGAGCTGT GTAACAACTA CATCCAGATG
    CACCTGGACC TGGAAAACAG TATGGAGGAG GCCCCAATCT TCAAGAGTGA CCCTTTCTTC
    ATCCTTCCCT CCTTCCAGTC GGAGTCCTCC ACCCCTTCCA CCGGGGGCTT CTCAGGGAAA
    GACACGCCTT CCGAGGATGA CCGCAGTCAG ACCCGGGATC ATGCGGGCGA GCCTCTGAGC
    CTGAGGGGGA CCAGCGGGGA GCTGCTGCTG CTGCCCCCGA GCCCCAAGGT GGAGAAGAAG
    GATCCCAGCC GCAAGAAGGA GTGGTGGGAG AACGCGGGCA ACAAAATCTA TACCATGGCA
    GCCGACAAGA CCATCTCAAA GCTGATGACC GAGTACAAGA GGAGGAAACA GCAGCACAAC
    CTGCCTGTCT TCCCCAAAGA GATCAAGGTG GAGAAGAAGG GAGAGGTGCT GGGCGCCAGG
    GGCCAAGGCT CCCCACTGCT CCAGCGACCC CAGCACCTGA TGGACCAGGG GCAGATGCGG
    CACTCTTTCA GCGCCGGCCC TGAGCTGCTG CGCCAGGAGA AGAGGCCCCG CTCCGGCTCC
    ACCGGCAGCT CCCGGAGTGT CTCCGCGAGG GACGCAGAAG CCCAGATCCA GGCATGGACC
    AACATGGTGC TAACAGTCCT CAATCAGATT CAGATCCTCC CAGACCAGAC CTTCACAGCC
    CTCCAGCCAG CAGTGTTTCC GTGTATCAGC CAGCTCACCT GTCACGTGAC TGACATCAGA
    GTTCGCCAGG CTGTGAGAGA ATGGCTCGGC AGAGTGGGCC GTGTCTATGA CATCATTATG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91509
    Clone ID Related Accession (Same CDS sequence) XM_015464839.1 , XM_015472886.1
    Accession Version XM_015464839.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645711.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAACGG ACTCTGATGA GAAGCAGCTG CTCAATCAGA TCCTGAACGC TGTGAAAGTG 
    ACGCCTTCCC TCCACGAAGA CCTGCAGGTG GAAGTGATGA AGGTTTTACT CTGCATAACC
    TACACTCCAA CCTTTGACCT GAATGGGAGT GCAGTGCTGA AGATCGCGGA GGTGTGCATT
    GAAACATACA TATGTAGCTG TCACCAGCGT AGTATAAACA CTGCTGTGCG GGCAACTCTC
    AGTCAAATGC TGAGTGACTT GACTTTACAG TTACGACAAA GGCAGGAGAA CACGATAATT
    GAAAACCCGG ATGTCCCCCA GGAATTCAGG AGTCAAGGGT CAACAGTAGA GTCCCTCTGT
    GATGACCTTG TCTCCGTGCT CACTGTTCTG TGTGAGAAGC TCCAAGCTGC CATCAATGAC
    AGCCAGCAGC TGCAGCTCCT GTACCTGGAG TGCATCCTGT CCGTGCTCAG CAGCTCCTCC
    TCCTCCATGC AGCTGCACCG AGGCTTCACA GACCTCATCT GGAAGAACCT TTGCCCCGCT
    CTCATCGTGA TCCTGGGGAA TCCGATTCAT GATAAAACCA TCACCTCTGC ACACAGCAGC
    AGCACGAGTA CCAGCATCGA GTCAGACTCT GTGTCTCCTG GGGTTTCCGA CCACGGCCGG
    GGTTCGGGCT GCTCCTCCAC AGCGCCAGCC CTGAGTGGGC CAGTGGCCCG GACCATCTAC
    TACATCGCGG CTGAGCTGGT GCGGCTGGTG GGGTCGGTGG ACTCCATGAA GCCCGTGTTG
    CAGTCCCTCT ACCACCGTGT GCTGCTCTAC CCCCCACCCC AGCACCGTGT GGAAGCCATC
    AAGATCATGA AAGAGCTACT TGGGAGCCCA AAGCGTCTCT GTGATTTGGC AGGACCCAGC
    TCCACTGAAC CTGACTCGAG AAAAAGATCA ATTTCAAAAA GAAAGTCTCA TCTGGATCTT
    CTCAAACTCA TCATGGACGG CATGACCGAG GCGTGTATCA AGGGCGGCAT CGAGGCCTGC
    TACGCCGCCG TGTCCTGCGT CTGCACCTTG CTGGGAGCCT TGGAGGAGCT CAGCCAGGGC
    AGGGGCTTGA GCGAAAGTCA AGTCCAGCTG CTGCTGCTGC GCCTGGAGGA GCTGAAGGAC
    GGGGCCGAGT CCAGCCGCGA CTCCATGGAG ATCAATGAGG CCGACTTCCG CTGGCAGCGG
    CGAGTCCTGT CCTCGGAACA CACACCCTGG GAGTTGGGGA ATGAGCGGAG CCTTGACATC
    AGCATCAGCG TTACCACGGA CACGGGCCAG ACCACCTTGG AGGGAGAGTT GGGTCAGACC
    ACACCCGAGG ACCACTCGGA AAACCCCAAG AATGGCCTCA AGTCACCAGC CGTCCAGGAG
    GGCAAGGAGA CTCTGAGTAA AGTGTCAGAA CCAGAAGCTG TGGACCAGCC AGACGTGGTT
    CAAAGGAGCC ACACGGTTGC CTACCCCGAT ATAACCAACT TCCTGTCCGT CGACTGCAGG
    ATGAGGTCCT ACGGATCCAG ATACAGTGAG AGCAACTTCA GCGTGGACGA CCAAGACCTC
    TCTCGGACAG AATTCGACTC CTGTGACCAG TACTCCATGG CGGCTGAAAA GGACTCCGGG
    AGATCGGACG TGTCGGACAT TGGATCCGAC AACTGTTCTT TAGCTGATGA GGAGCAGACC
    CCCCGGGACT GCCTGGGCCA CAGGTCCCTG CGGACTGCAG CTCTGTCTCT CAAGCTGCTG
    AAGAACCAGG AGGCTGACCA GCACAGCGCC CGGCTGTTCG TGCAGTCGCT GGAGGGTCTC
    CTGCCGCGCC TCCTGGCCCT GCCCAGTGTG GAAGAGGTGG ACTCAGCCCT CCAGAACTTT
    GTCTCTACCT TCTGCTCAGG TATGATGCAT TCACCAGGGT TTGATGGGAA CAGCAGCCTG
    GGCCTGCAGA TGCTGATGAA CGCGGACAGC CTGTACTCGG CGGCGCACTG CGCGCTGCTG
    CTCAACCTGA AGCTCTCCCA CGGTGACTAC TACCGCAAGC GGCCCACCCT AGCACCCAGC
    ACCATGAAAG AGTTCATGAA GCAGGTCCAG ACCAGCGGGG TGCTGATGGT CTTCTCCCAG
    GCCTGGATTG AGGAACTTTA CCACCAGGTT CTTGACAGAA ACATGCTCGG AGAAGCCGGC
    TACTGGGGCA GCCCTGAAGA CAACAGCCTC CCCCTCATCA CCATGCTGAC GGATATCGAT
    GGCTTAGAGA GCAGTGCAAT TGGAGGGCAG CTGATGGCCT CAGCTTCGAT GGAGTCTCCT
    TTCACCCAGG GCAGGAGAAT TGATGACTCC ACGGTGGCAG GTGTGGCATT TGCTCGCTAT
    ATTCTGATTG GCTGCTGGAA GAACTTGATC GACACTTTAT CAACCCCCCT GACGGGCCGA
    ATGGCCGGCA GCTCCAAAGG GCTGGCCTTC ATCTTGGGAG CTGAAGGCAT CAAAGAACAG
    AACCAAAAAG AACGCGATGC TATCTGCATG AGCCTCGACG GGCTACGGAA AGCCGCGCGT
    CTGAGCTGCG CTCTAGGAGT TGCAGCCAAC TGTGCTTCGG CCCTCGCCCA GATGGCAGCT
    GCCTCCTGTG TCCAGGAGGA GAAAGAGGAA AAGGAAACCC AGGAACCCAG TGATGCCATA
    GCACAAGTGA AACTAAAAGT GGAGCAGAAG CTGGAGCAGA TCGGGAAGCT GCAGGGGGTG
    TGGCTGCACA CAGCCCACGT CCTGTGCATG GACGCCATCC TCAGTGTCGG CCTGGAGATG
    GGCAGCCACA ACCCCGACTG CTGGCCACAC GTGTTCAGGG TGTGCGAGTA CGTGGGCACC
    TTGGAACACA CCCACTTCAG CGATGGCACC TCTCAGCCCC CGCTGACCAT CAGCCAGCCC
    CAGAAGGCCC CCAGAGGCGG ACTCCTTGGG GACCTGGAGA GTGAGGGCTC AGCCCAGGAG
    CAGAGCCTGG AGCAGGAGAG CTCCCTGAGC GCGGCCCCCG TCATCCAACC GCTCTCCATC
    CAGGAGCTCG TCCGAGAAGG CAGCCGAGGC CGGGCCTCTG ACTTCCGTGG GGGCAGCCTC
    ATGACAGGCA GCAGTGCCGC CAAGGTGGTG CTCACGCTCT CCACGCAGGC TGACAGGCTC
    TTTGAAGATG CTACAGATAA ACTGAACCTC ATGGCTCTGG GAGGTTTTCT TTACCAGCTG
    AAGAAAGCAT CGCAGTCCCA GCTTTTCCAT TCTGTTACGG AAACAGTTGA TTACTCGCTG
    GCAATGCCAG GAGAAGTGAA GTCTACCCAA GACCGCAAAA GTGCCCTCCA CCTGTTCCGC
    CTCGGGGACG CCATGCTAAG GATCGTGAGG AGCAAATCGA GGCCCCTGCT CCACGTGATG
    CGCTGCTGGA GCCTCGTGGC CCCACACCTG GTAGAGGCTG CCTGCCATAA GGAAAGACAC
    GTGTCTCAGA AGGCGGTTTC CTTCATCCAT GACATACTGA CGGAGGTTCT CACGGACTGG
    AATGAGCCAC CTCATTTCCA CTTTAACGAA GCTCTCTTCC GACCGTTCGA ACGTATCATG
    CAGCTGGAGT TGTGTGACGA GGACGTCCAA GACCAGGTTG TCACGTCCAT CGGGGAGCTG
    GTTGAAGTGT GCTCTGCCCA GATCCAGTCA GGCTGGAGGC CCCTGTTCAG CGCCCTGGAA
    ACGGTGCACA GCGGGAACAA GTCCGAGGTG AAGGAGTACC TGGTGGGCGA CTATTCCATG
    GGAAAAGGCC AGGCTCCAGT GTTTGATGTA TTTGAAGCTT TTCTCAATAC CGACAACATC
    CAGGTCTTTG CTAATGCAGC CACTAGTTAC ATCATGTGCC TTATGAAGTT TGTCAAAGGA
    CTGGGGGAGG TGGACTGTAA GGAGATTGGA GACTGTGTCC CAGGACCCGG AGCCGCGTCC
    CCAGACCTGT GCCTCCCCGC CCTGGACTAC CTCCGGCGCT GTTCTCAGTT GTTGGCCAAA
    ATCTACAAAA TGCCCTTGAA GCCAATATTC CTTAGTGGGC GACTTGCTAG CTTGCCCCGA
    AGACTTCAGG AGCAGTCAGC GAGCAGTGAG GATGGAATTG AGTCAGTCTT GTCTGACTTT
    GATGACGACA CTGGTCTGAT CGAAGTTTGG ATCATCCTCT TGGAGCAGCT CACAGCAGCT
    GTGTCCAACT GTCCACGTCA GCACCAACCC CCCACTCTGG ATTTGCTCTT TGAGCTGTTG
    AGAGAGGTTA CCAAAACACC TGGACCAGGG TTTGGCATCT ATGCAGTTGT TCACCTTCTC
    CTTCCTGTGA TGTCTCTTTG GCTTCGCCGT AGCCATAAAG ACCATTCTTA CTGGGACATT
    GCCTCGGCTA ACTTCAAGCA TGCCATTGGT CTGTCCTGTG AGCTGGTGGT GGAGCACATT
    CAGAGCTTTA TCCACTCAGA CATCAGGTAT GAGAGCATGA TCAACACCAT GCTGAAGGAC
    CTCTTTGAGC TACTGGTAGC ATGTGTAGCC AAGCCCACAG AAACCATCTC CCGAGTAGGC
    TGCTCCTGTA TTAGGTACGT CCTCGTGACA GCGGGCCCCG TGTTCACGGA GGAGATGTGG
    AGGCTGGCCT GCTGTGCCCT GCAGGACGCC TTCTCGGCCA CACTCAAGCC GGTGAAGGAC
    CTGCTGGGCT GCTTCCACAG TGGCACCGAG AGCTTCAGCG GCGAAGGCTG CCAGGTGAGG
    GTGGCCGCCC CGTCCTCCTC CCCGAGTGCC GAGGCCGAGT ACTGGCGCAT CCGAGCCATG
    GCTCAGCAGG TATTTATGTT GGATACCCAG TGCTCACCGA AGACTCCAAA CAACTTCGAT
    CATGCTCAGT CCTGTCAGCT CATTATTGAA CTGCCTCCTG ATGAGAAGCC AAATGGGCAT
    ACCAAGAAAA GTGTTTCTTT CAGGGAAATT GTGGTGAGTT TACTATCCCA TCAAGTATTA
    CTCCAGAACT TATATGATAT CTTATTAGAA GAGTTTGTCA AAGGCCCCTC TCCTGGGGAG
    GAGAAGATCC AAGGGCCAGA AACCAAGCTG GCTGGCTTCC TCAGATACAT CTCCATGCAG
    AACCTGGCCG TCATCTTTGA CCTTCTGCTG GACTCCTACA GGACGGCCAG AGAGTTTGAC
    ACCAGCCCAG GGCTGAAGTG CCTGCTGAAG AAAGTGTCTG GCATAGGGGG CGCTGCCAAC
    CTCTACCGCC AGTCTGCCAT GAGTTTCAAC ATTTATTTCC ACGCACTGGT CTGTGCTGTT
    CTTAGCAACC AAGAAACCAT CACTGCTGAC CAAGTGAAGA AGGTCCTTTT TGAGGACGAT
    GAGAGGAGCA CAGATTCGTC CCAGCAGTGT TCATCAGAAG ATGAAGACAT TTTTGAGGAG
    ACTGCCCAGG TGAGCCCCCC GAGAGGCAAA GAAAAGAGGC AGTGGAGGGC TCGGATGCCC
    TCGCTCAGCG TCCAGCCCGT CAGCAACGCG GACTGGGTGT GGCTGGTCAA GAGGCTTCAC
    AAGCTGTGCA TGGAGCTGTG TAACAACTAC ATCCAGATGC ACCTGGACCT GGAAAACAGT
    ATGGAGGAGG CCCCAATCTT CAAGAGTGAC CCTTTCTTCA TCCTTCCCTC CTTCCAGTCG
    GAGTCCTCCA CCCCTTCCAC CGGGGGCTTC TCAGGGAAAG ACACGCCTTC CGAGGATGAC
    CGCAGTCAGA CCCGGGATCA TGCGGGCGAG CCTCTGAGCC TGAGGGGGAC CAGCGGGGAG
    CTGCTGCTGC TGCCCCCGAG CCCCAAGGTG GAGAAGAAGG ATCCCAGCCG CAAGAAGGAG
    TGGTGGGAGA ACGCGGGCAA CAAAATCTAT ACCATGGCAG CCGACAAGAC CATCTCAAAG
    CTGATGACCG AGTACAAGAG GAGGAAACAG CAGCACAACC TGCCTGTCTT CCCCAAAGAG
    ATCAAGGTGG AGAAGAAGGG AGAGGTGCTG GGCGCCAGGG GCCAAGGCTC CCCACTGCTC
    CAGCGACCCC AGCACCTGAT GGACCAGGGG CAGATGCGGC ACTCTTTCAG CGCCGGCCCT
    GAGCTGCTGC GCCAGGAGAA GAGGCCCCGC TCCGGCTCCA CCGGCAGCTC CCGGAGTGTC
    TCCGCGAGGG ACGCAGAAGC CCAGATCCAG GCATGGACCA ACATGGTGCT AACAGTCCTC
    AATCAGATTC AGATCCTCCC AGACCAGACC TTCACAGCCC TCCAGCCAGC AGTGTTTCCG
    TGTATCAGCC AGCTCACCTG TCACGTGACT GACATCAGAG TTCGCCAGGC TGTGAGAGAA
    TGGCTCGGCA GAGTGGGCCG TGTCTATGAC ATCATTATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320325.1
    CDS1094..7375
    Translation

    Target ORF information:

    RefSeq Version XM_015464839.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464839.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAACGG ACTCTGATGA GAAGCAGCTG CTCAATCAGA TCCTGAACGC TGTGAAAGTG 
    ACGCCTTCCC TCCACGAAGA CCTGCAGGTG GAAGTGATGA AGGTTTTACT CTGCATAACC
    TACACTCCAA CCTTTGACCT GAATGGGAGT GCAGTGCTGA AGATCGCGGA GGTGTGCATT
    GAAACATACA TATGTAGCTG TCACCAGCGT AGTATAAACA CTGCTGTGCG GGCAACTCTC
    AGTCAAATGC TGAGTGACTT GACTTTACAG TTACGACAAA GGCAGGAGAA CACGATAATT
    GAAAACCCGG ATGTCCCCCA GGAATTCAGG AGTCAAGGGT CAACAGTAGA GTCCCTCTGT
    GATGACCTTG TCTCCGTGCT CACTGTTCTG TGTGAGAAGC TCCAAGCTGC CATCAATGAC
    AGCCAGCAGC TGCAGCTCCT GTACCTGGAG TGCATCCTGT CCGTGCTCAG CAGCTCCTCC
    TCCTCCATGC AGCTGCACCG AGGCTTCACA GACCTCATCT GGAAGAACCT TTGCCCCGCT
    CTCATCGTGA TCCTGGGGAA TCCGATTCAT GATAAAACCA TCACCTCTGC ACACAGCAGC
    AGCACGAGTA CCAGCATCGA GTCAGACTCT GTGTCTCCTG GGGTTTCCGA CCACGGCCGG
    GGTTCGGGCT GCTCCTCCAC AGCGCCAGCC CTGAGTGGGC CAGTGGCCCG GACCATCTAC
    TACATCGCGG CTGAGCTGGT GCGGCTGGTG GGGTCGGTGG ACTCCATGAA GCCCGTGTTG
    CAGTCCCTCT ACCACCGTGT GCTGCTCTAC CCCCCACCCC AGCACCGTGT GGAAGCCATC
    AAGATCATGA AAGAGCTACT TGGGAGCCCA AAGCGTCTCT GTGATTTGGC AGGACCCAGC
    TCCACTGAAC CTGACTCGAG AAAAAGATCA ATTTCAAAAA GAAAGTCTCA TCTGGATCTT
    CTCAAACTCA TCATGGACGG CATGACCGAG GCGTGTATCA AGGGCGGCAT CGAGGCCTGC
    TACGCCGCCG TGTCCTGCGT CTGCACCTTG CTGGGAGCCT TGGAGGAGCT CAGCCAGGGC
    AGGGGCTTGA GCGAAAGTCA AGTCCAGCTG CTGCTGCTGC GCCTGGAGGA GCTGAAGGAC
    GGGGCCGAGT CCAGCCGCGA CTCCATGGAG ATCAATGAGG CCGACTTCCG CTGGCAGCGG
    CGAGTCCTGT CCTCGGAACA CACACCCTGG GAGTTGGGGA ATGAGCGGAG CCTTGACATC
    AGCATCAGCG TTACCACGGA CACGGGCCAG ACCACCTTGG AGGGAGAGTT GGGTCAGACC
    ACACCCGAGG ACCACTCGGA AAACCCCAAG AATGGCCTCA AGTCACCAGC CGTCCAGGAG
    GGCAAGGAGA CTCTGAGTAA AGTGTCAGAA CCAGAAGCTG TGGACCAGCC AGACGTGGTT
    CAAAGGAGCC ACACGGTTGC CTACCCCGAT ATAACCAACT TCCTGTCCGT CGACTGCAGG
    ATGAGGTCCT ACGGATCCAG ATACAGTGAG AGCAACTTCA GCGTGGACGA CCAAGACCTC
    TCTCGGACAG AATTCGACTC CTGTGACCAG TACTCCATGG CGGCTGAAAA GGACTCCGGG
    AGATCGGACG TGTCGGACAT TGGATCCGAC AACTGTTCTT TAGCTGATGA GGAGCAGACC
    CCCCGGGACT GCCTGGGCCA CAGGTCCCTG CGGACTGCAG CTCTGTCTCT CAAGCTGCTG
    AAGAACCAGG AGGCTGACCA GCACAGCGCC CGGCTGTTCG TGCAGTCGCT GGAGGGTCTC
    CTGCCGCGCC TCCTGGCCCT GCCCAGTGTG GAAGAGGTGG ACTCAGCCCT CCAGAACTTT
    GTCTCTACCT TCTGCTCAGG TATGATGCAT TCACCAGGGT TTGATGGGAA CAGCAGCCTG
    GGCCTGCAGA TGCTGATGAA CGCGGACAGC CTGTACTCGG CGGCGCACTG CGCGCTGCTG
    CTCAACCTGA AGCTCTCCCA CGGTGACTAC TACCGCAAGC GGCCCACCCT AGCACCCAGC
    ACCATGAAAG AGTTCATGAA GCAGGTCCAG ACCAGCGGGG TGCTGATGGT CTTCTCCCAG
    GCCTGGATTG AGGAACTTTA CCACCAGGTT CTTGACAGAA ACATGCTCGG AGAAGCCGGC
    TACTGGGGCA GCCCTGAAGA CAACAGCCTC CCCCTCATCA CCATGCTGAC GGATATCGAT
    GGCTTAGAGA GCAGTGCAAT TGGAGGGCAG CTGATGGCCT CAGCTTCGAT GGAGTCTCCT
    TTCACCCAGG GCAGGAGAAT TGATGACTCC ACGGTGGCAG GTGTGGCATT TGCTCGCTAT
    ATTCTGATTG GCTGCTGGAA GAACTTGATC GACACTTTAT CAACCCCCCT GACGGGCCGA
    ATGGCCGGCA GCTCCAAAGG GCTGGCCTTC ATCTTGGGAG CTGAAGGCAT CAAAGAACAG
    AACCAAAAAG AACGCGATGC TATCTGCATG AGCCTCGACG GGCTACGGAA AGCCGCGCGT
    CTGAGCTGCG CTCTAGGAGT TGCAGCCAAC TGTGCTTCGG CCCTCGCCCA GATGGCAGCT
    GCCTCCTGTG TCCAGGAGGA GAAAGAGGAA AAGGAAACCC AGGAACCCAG TGATGCCATA
    GCACAAGTGA AACTAAAAGT GGAGCAGAAG CTGGAGCAGA TCGGGAAGCT GCAGGGGGTG
    TGGCTGCACA CAGCCCACGT CCTGTGCATG GACGCCATCC TCAGTGTCGG CCTGGAGATG
    GGCAGCCACA ACCCCGACTG CTGGCCACAC GTGTTCAGGG TGTGCGAGTA CGTGGGCACC
    TTGGAACACA CCCACTTCAG CGATGGCACC TCTCAGCCCC CGCTGACCAT CAGCCAGCCC
    CAGAAGGCCC CCAGAGGCGG ACTCCTTGGG GACCTGGAGA GTGAGGGCTC AGCCCAGGAG
    CAGAGCCTGG AGCAGGAGAG CTCCCTGAGC GCGGCCCCCG TCATCCAACC GCTCTCCATC
    CAGGAGCTCG TCCGAGAAGG CAGCCGAGGC CGGGCCTCTG ACTTCCGTGG GGGCAGCCTC
    ATGACAGGCA GCAGTGCCGC CAAGGTGGTG CTCACGCTCT CCACGCAGGC TGACAGGCTC
    TTTGAAGATG CTACAGATAA ACTGAACCTC ATGGCTCTGG GAGGTTTTCT TTACCAGCTG
    AAGAAAGCAT CGCAGTCCCA GCTTTTCCAT TCTGTTACGG AAACAGTTGA TTACTCGCTG
    GCAATGCCAG GAGAAGTGAA GTCTACCCAA GACCGCAAAA GTGCCCTCCA CCTGTTCCGC
    CTCGGGGACG CCATGCTAAG GATCGTGAGG AGCAAATCGA GGCCCCTGCT CCACGTGATG
    CGCTGCTGGA GCCTCGTGGC CCCACACCTG GTAGAGGCTG CCTGCCATAA GGAAAGACAC
    GTGTCTCAGA AGGCGGTTTC CTTCATCCAT GACATACTGA CGGAGGTTCT CACGGACTGG
    AATGAGCCAC CTCATTTCCA CTTTAACGAA GCTCTCTTCC GACCGTTCGA ACGTATCATG
    CAGCTGGAGT TGTGTGACGA GGACGTCCAA GACCAGGTTG TCACGTCCAT CGGGGAGCTG
    GTTGAAGTGT GCTCTGCCCA GATCCAGTCA GGCTGGAGGC CCCTGTTCAG CGCCCTGGAA
    ACGGTGCACA GCGGGAACAA GTCCGAGGTG AAGGAGTACC TGGTGGGCGA CTATTCCATG
    GGAAAAGGCC AGGCTCCAGT GTTTGATGTA TTTGAAGCTT TTCTCAATAC CGACAACATC
    CAGGTCTTTG CTAATGCAGC CACTAGTTAC ATCATGTGCC TTATGAAGTT TGTCAAAGGA
    CTGGGGGAGG TGGACTGTAA GGAGATTGGA GACTGTGTCC CAGGACCCGG AGCCGCGTCC
    CCAGACCTGT GCCTCCCCGC CCTGGACTAC CTCCGGCGCT GTTCTCAGTT GTTGGCCAAA
    ATCTACAAAA TGCCCTTGAA GCCAATATTC CTTAGTGGGC GACTTGCTAG CTTGCCCCGA
    AGACTTCAGG AGCAGTCAGC GAGCAGTGAG GATGGAATTG AGTCAGTCTT GTCTGACTTT
    GATGACGACA CTGGTCTGAT CGAAGTTTGG ATCATCCTCT TGGAGCAGCT CACAGCAGCT
    GTGTCCAACT GTCCACGTCA GCACCAACCC CCCACTCTGG ATTTGCTCTT TGAGCTGTTG
    AGAGAGGTTA CCAAAACACC TGGACCAGGG TTTGGCATCT ATGCAGTTGT TCACCTTCTC
    CTTCCTGTGA TGTCTCTTTG GCTTCGCCGT AGCCATAAAG ACCATTCTTA CTGGGACATT
    GCCTCGGCTA ACTTCAAGCA TGCCATTGGT CTGTCCTGTG AGCTGGTGGT GGAGCACATT
    CAGAGCTTTA TCCACTCAGA CATCAGGTAT GAGAGCATGA TCAACACCAT GCTGAAGGAC
    CTCTTTGAGC TACTGGTAGC ATGTGTAGCC AAGCCCACAG AAACCATCTC CCGAGTAGGC
    TGCTCCTGTA TTAGGTACGT CCTCGTGACA GCGGGCCCCG TGTTCACGGA GGAGATGTGG
    AGGCTGGCCT GCTGTGCCCT GCAGGACGCC TTCTCGGCCA CACTCAAGCC GGTGAAGGAC
    CTGCTGGGCT GCTTCCACAG TGGCACCGAG AGCTTCAGCG GCGAAGGCTG CCAGGTGAGG
    GTGGCCGCCC CGTCCTCCTC CCCGAGTGCC GAGGCCGAGT ACTGGCGCAT CCGAGCCATG
    GCTCAGCAGG TATTTATGTT GGATACCCAG TGCTCACCGA AGACTCCAAA CAACTTCGAT
    CATGCTCAGT CCTGTCAGCT CATTATTGAA CTGCCTCCTG ATGAGAAGCC AAATGGGCAT
    ACCAAGAAAA GTGTTTCTTT CAGGGAAATT GTGGTGAGTT TACTATCCCA TCAAGTATTA
    CTCCAGAACT TATATGATAT CTTATTAGAA GAGTTTGTCA AAGGCCCCTC TCCTGGGGAG
    GAGAAGATCC AAGGGCCAGA AACCAAGCTG GCTGGCTTCC TCAGATACAT CTCCATGCAG
    AACCTGGCCG TCATCTTTGA CCTTCTGCTG GACTCCTACA GGACGGCCAG AGAGTTTGAC
    ACCAGCCCAG GGCTGAAGTG CCTGCTGAAG AAAGTGTCTG GCATAGGGGG CGCTGCCAAC
    CTCTACCGCC AGTCTGCCAT GAGTTTCAAC ATTTATTTCC ACGCACTGGT CTGTGCTGTT
    CTTAGCAACC AAGAAACCAT CACTGCTGAC CAAGTGAAGA AGGTCCTTTT TGAGGACGAT
    GAGAGGAGCA CAGATTCGTC CCAGCAGTGT TCATCAGAAG ATGAAGACAT TTTTGAGGAG
    ACTGCCCAGG TGAGCCCCCC GAGAGGCAAA GAAAAGAGGC AGTGGAGGGC TCGGATGCCC
    TCGCTCAGCG TCCAGCCCGT CAGCAACGCG GACTGGGTGT GGCTGGTCAA GAGGCTTCAC
    AAGCTGTGCA TGGAGCTGTG TAACAACTAC ATCCAGATGC ACCTGGACCT GGAAAACAGT
    ATGGAGGAGG CCCCAATCTT CAAGAGTGAC CCTTTCTTCA TCCTTCCCTC CTTCCAGTCG
    GAGTCCTCCA CCCCTTCCAC CGGGGGCTTC TCAGGGAAAG ACACGCCTTC CGAGGATGAC
    CGCAGTCAGA CCCGGGATCA TGCGGGCGAG CCTCTGAGCC TGAGGGGGAC CAGCGGGGAG
    CTGCTGCTGC TGCCCCCGAG CCCCAAGGTG GAGAAGAAGG ATCCCAGCCG CAAGAAGGAG
    TGGTGGGAGA ACGCGGGCAA CAAAATCTAT ACCATGGCAG CCGACAAGAC CATCTCAAAG
    CTGATGACCG AGTACAAGAG GAGGAAACAG CAGCACAACC TGCCTGTCTT CCCCAAAGAG
    ATCAAGGTGG AGAAGAAGGG AGAGGTGCTG GGCGCCAGGG GCCAAGGCTC CCCACTGCTC
    CAGCGACCCC AGCACCTGAT GGACCAGGGG CAGATGCGGC ACTCTTTCAG CGCCGGCCCT
    GAGCTGCTGC GCCAGGAGAA GAGGCCCCGC TCCGGCTCCA CCGGCAGCTC CCGGAGTGTC
    TCCGCGAGGG ACGCAGAAGC CCAGATCCAG GCATGGACCA ACATGGTGCT AACAGTCCTC
    AATCAGATTC AGATCCTCCC AGACCAGACC TTCACAGCCC TCCAGCCAGC AGTGTTTCCG
    TGTATCAGCC AGCTCACCTG TCACGTGACT GACATCAGAG TTCGCCAGGC TGTGAGAGAA
    TGGCTCGGCA GAGTGGGCCG TGTCTATGAC ATCATTATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91509
    Clone ID Related Accession (Same CDS sequence) XM_015464839.1 , XM_015472886.1
    Accession Version XM_015472886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104223.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAACGG ACTCTGATGA GAAGCAGCTG CTCAATCAGA TCCTGAACGC TGTGAAAGTG 
    ACGCCTTCCC TCCACGAAGA CCTGCAGGTG GAAGTGATGA AGGTTTTACT CTGCATAACC
    TACACTCCAA CCTTTGACCT GAATGGGAGT GCAGTGCTGA AGATCGCGGA GGTGTGCATT
    GAAACATACA TATGTAGCTG TCACCAGCGT AGTATAAACA CTGCTGTGCG GGCAACTCTC
    AGTCAAATGC TGAGTGACTT GACTTTACAG TTACGACAAA GGCAGGAGAA CACGATAATT
    GAAAACCCGG ATGTCCCCCA GGAATTCAGG AGTCAAGGGT CAACAGTAGA GTCCCTCTGT
    GATGACCTTG TCTCCGTGCT CACTGTTCTG TGTGAGAAGC TCCAAGCTGC CATCAATGAC
    AGCCAGCAGC TGCAGCTCCT GTACCTGGAG TGCATCCTGT CCGTGCTCAG CAGCTCCTCC
    TCCTCCATGC AGCTGCACCG AGGCTTCACA GACCTCATCT GGAAGAACCT TTGCCCCGCT
    CTCATCGTGA TCCTGGGGAA TCCGATTCAT GATAAAACCA TCACCTCTGC ACACAGCAGC
    AGCACGAGTA CCAGCATCGA GTCAGACTCT GTGTCTCCTG GGGTTTCCGA CCACGGCCGG
    GGTTCGGGCT GCTCCTCCAC AGCGCCAGCC CTGAGTGGGC CAGTGGCCCG GACCATCTAC
    TACATCGCGG CTGAGCTGGT GCGGCTGGTG GGGTCGGTGG ACTCCATGAA GCCCGTGTTG
    CAGTCCCTCT ACCACCGTGT GCTGCTCTAC CCCCCACCCC AGCACCGTGT GGAAGCCATC
    AAGATCATGA AAGAGCTACT TGGGAGCCCA AAGCGTCTCT GTGATTTGGC AGGACCCAGC
    TCCACTGAAC CTGACTCGAG AAAAAGATCA ATTTCAAAAA GAAAGTCTCA TCTGGATCTT
    CTCAAACTCA TCATGGACGG CATGACCGAG GCGTGTATCA AGGGCGGCAT CGAGGCCTGC
    TACGCCGCCG TGTCCTGCGT CTGCACCTTG CTGGGAGCCT TGGAGGAGCT CAGCCAGGGC
    AGGGGCTTGA GCGAAAGTCA AGTCCAGCTG CTGCTGCTGC GCCTGGAGGA GCTGAAGGAC
    GGGGCCGAGT CCAGCCGCGA CTCCATGGAG ATCAATGAGG CCGACTTCCG CTGGCAGCGG
    CGAGTCCTGT CCTCGGAACA CACACCCTGG GAGTTGGGGA ATGAGCGGAG CCTTGACATC
    AGCATCAGCG TTACCACGGA CACGGGCCAG ACCACCTTGG AGGGAGAGTT GGGTCAGACC
    ACACCCGAGG ACCACTCGGA AAACCCCAAG AATGGCCTCA AGTCACCAGC CGTCCAGGAG
    GGCAAGGAGA CTCTGAGTAA AGTGTCAGAA CCAGAAGCTG TGGACCAGCC AGACGTGGTT
    CAAAGGAGCC ACACGGTTGC CTACCCCGAT ATAACCAACT TCCTGTCCGT CGACTGCAGG
    ATGAGGTCCT ACGGATCCAG ATACAGTGAG AGCAACTTCA GCGTGGACGA CCAAGACCTC
    TCTCGGACAG AATTCGACTC CTGTGACCAG TACTCCATGG CGGCTGAAAA GGACTCCGGG
    AGATCGGACG TGTCGGACAT TGGATCCGAC AACTGTTCTT TAGCTGATGA GGAGCAGACC
    CCCCGGGACT GCCTGGGCCA CAGGTCCCTG CGGACTGCAG CTCTGTCTCT CAAGCTGCTG
    AAGAACCAGG AGGCTGACCA GCACAGCGCC CGGCTGTTCG TGCAGTCGCT GGAGGGTCTC
    CTGCCGCGCC TCCTGGCCCT GCCCAGTGTG GAAGAGGTGG ACTCAGCCCT CCAGAACTTT
    GTCTCTACCT TCTGCTCAGG TATGATGCAT TCACCAGGGT TTGATGGGAA CAGCAGCCTG
    GGCCTGCAGA TGCTGATGAA CGCGGACAGC CTGTACTCGG CGGCGCACTG CGCGCTGCTG
    CTCAACCTGA AGCTCTCCCA CGGTGACTAC TACCGCAAGC GGCCCACCCT AGCACCCAGC
    ACCATGAAAG AGTTCATGAA GCAGGTCCAG ACCAGCGGGG TGCTGATGGT CTTCTCCCAG
    GCCTGGATTG AGGAACTTTA CCACCAGGTT CTTGACAGAA ACATGCTCGG AGAAGCCGGC
    TACTGGGGCA GCCCTGAAGA CAACAGCCTC CCCCTCATCA CCATGCTGAC GGATATCGAT
    GGCTTAGAGA GCAGTGCAAT TGGAGGGCAG CTGATGGCCT CAGCTTCGAT GGAGTCTCCT
    TTCACCCAGG GCAGGAGAAT TGATGACTCC ACGGTGGCAG GTGTGGCATT TGCTCGCTAT
    ATTCTGATTG GCTGCTGGAA GAACTTGATC GACACTTTAT CAACCCCCCT GACGGGCCGA
    ATGGCCGGCA GCTCCAAAGG GCTGGCCTTC ATCTTGGGAG CTGAAGGCAT CAAAGAACAG
    AACCAAAAAG AACGCGATGC TATCTGCATG AGCCTCGACG GGCTACGGAA AGCCGCGCGT
    CTGAGCTGCG CTCTAGGAGT TGCAGCCAAC TGTGCTTCGG CCCTCGCCCA GATGGCAGCT
    GCCTCCTGTG TCCAGGAGGA GAAAGAGGAA AAGGAAACCC AGGAACCCAG TGATGCCATA
    GCACAAGTGA AACTAAAAGT GGAGCAGAAG CTGGAGCAGA TCGGGAAGCT GCAGGGGGTG
    TGGCTGCACA CAGCCCACGT CCTGTGCATG GACGCCATCC TCAGTGTCGG CCTGGAGATG
    GGCAGCCACA ACCCCGACTG CTGGCCACAC GTGTTCAGGG TGTGCGAGTA CGTGGGCACC
    TTGGAACACA CCCACTTCAG CGATGGCACC TCTCAGCCCC CGCTGACCAT CAGCCAGCCC
    CAGAAGGCCC CCAGAGGCGG ACTCCTTGGG GACCTGGAGA GTGAGGGCTC AGCCCAGGAG
    CAGAGCCTGG AGCAGGAGAG CTCCCTGAGC GCGGCCCCCG TCATCCAACC GCTCTCCATC
    CAGGAGCTCG TCCGAGAAGG CAGCCGAGGC CGGGCCTCTG ACTTCCGTGG GGGCAGCCTC
    ATGACAGGCA GCAGTGCCGC CAAGGTGGTG CTCACGCTCT CCACGCAGGC TGACAGGCTC
    TTTGAAGATG CTACAGATAA ACTGAACCTC ATGGCTCTGG GAGGTTTTCT TTACCAGCTG
    AAGAAAGCAT CGCAGTCCCA GCTTTTCCAT TCTGTTACGG AAACAGTTGA TTACTCGCTG
    GCAATGCCAG GAGAAGTGAA GTCTACCCAA GACCGCAAAA GTGCCCTCCA CCTGTTCCGC
    CTCGGGGACG CCATGCTAAG GATCGTGAGG AGCAAATCGA GGCCCCTGCT CCACGTGATG
    CGCTGCTGGA GCCTCGTGGC CCCACACCTG GTAGAGGCTG CCTGCCATAA GGAAAGACAC
    GTGTCTCAGA AGGCGGTTTC CTTCATCCAT GACATACTGA CGGAGGTTCT CACGGACTGG
    AATGAGCCAC CTCATTTCCA CTTTAACGAA GCTCTCTTCC GACCGTTCGA ACGTATCATG
    CAGCTGGAGT TGTGTGACGA GGACGTCCAA GACCAGGTTG TCACGTCCAT CGGGGAGCTG
    GTTGAAGTGT GCTCTGCCCA GATCCAGTCA GGCTGGAGGC CCCTGTTCAG CGCCCTGGAA
    ACGGTGCACA GCGGGAACAA GTCCGAGGTG AAGGAGTACC TGGTGGGCGA CTATTCCATG
    GGAAAAGGCC AGGCTCCAGT GTTTGATGTA TTTGAAGCTT TTCTCAATAC CGACAACATC
    CAGGTCTTTG CTAATGCAGC CACTAGTTAC ATCATGTGCC TTATGAAGTT TGTCAAAGGA
    CTGGGGGAGG TGGACTGTAA GGAGATTGGA GACTGTGTCC CAGGACCCGG AGCCGCGTCC
    CCAGACCTGT GCCTCCCCGC CCTGGACTAC CTCCGGCGCT GTTCTCAGTT GTTGGCCAAA
    ATCTACAAAA TGCCCTTGAA GCCAATATTC CTTAGTGGGC GACTTGCTAG CTTGCCCCGA
    AGACTTCAGG AGCAGTCAGC GAGCAGTGAG GATGGAATTG AGTCAGTCTT GTCTGACTTT
    GATGACGACA CTGGTCTGAT CGAAGTTTGG ATCATCCTCT TGGAGCAGCT CACAGCAGCT
    GTGTCCAACT GTCCACGTCA GCACCAACCC CCCACTCTGG ATTTGCTCTT TGAGCTGTTG
    AGAGAGGTTA CCAAAACACC TGGACCAGGG TTTGGCATCT ATGCAGTTGT TCACCTTCTC
    CTTCCTGTGA TGTCTCTTTG GCTTCGCCGT AGCCATAAAG ACCATTCTTA CTGGGACATT
    GCCTCGGCTA ACTTCAAGCA TGCCATTGGT CTGTCCTGTG AGCTGGTGGT GGAGCACATT
    CAGAGCTTTA TCCACTCAGA CATCAGGTAT GAGAGCATGA TCAACACCAT GCTGAAGGAC
    CTCTTTGAGC TACTGGTAGC ATGTGTAGCC AAGCCCACAG AAACCATCTC CCGAGTAGGC
    TGCTCCTGTA TTAGGTACGT CCTCGTGACA GCGGGCCCCG TGTTCACGGA GGAGATGTGG
    AGGCTGGCCT GCTGTGCCCT GCAGGACGCC TTCTCGGCCA CACTCAAGCC GGTGAAGGAC
    CTGCTGGGCT GCTTCCACAG TGGCACCGAG AGCTTCAGCG GCGAAGGCTG CCAGGTGAGG
    GTGGCCGCCC CGTCCTCCTC CCCGAGTGCC GAGGCCGAGT ACTGGCGCAT CCGAGCCATG
    GCTCAGCAGG TATTTATGTT GGATACCCAG TGCTCACCGA AGACTCCAAA CAACTTCGAT
    CATGCTCAGT CCTGTCAGCT CATTATTGAA CTGCCTCCTG ATGAGAAGCC AAATGGGCAT
    ACCAAGAAAA GTGTTTCTTT CAGGGAAATT GTGGTGAGTT TACTATCCCA TCAAGTATTA
    CTCCAGAACT TATATGATAT CTTATTAGAA GAGTTTGTCA AAGGCCCCTC TCCTGGGGAG
    GAGAAGATCC AAGGGCCAGA AACCAAGCTG GCTGGCTTCC TCAGATACAT CTCCATGCAG
    AACCTGGCCG TCATCTTTGA CCTTCTGCTG GACTCCTACA GGACGGCCAG AGAGTTTGAC
    ACCAGCCCAG GGCTGAAGTG CCTGCTGAAG AAAGTGTCTG GCATAGGGGG CGCTGCCAAC
    CTCTACCGCC AGTCTGCCAT GAGTTTCAAC ATTTATTTCC ACGCACTGGT CTGTGCTGTT
    CTTAGCAACC AAGAAACCAT CACTGCTGAC CAAGTGAAGA AGGTCCTTTT TGAGGACGAT
    GAGAGGAGCA CAGATTCGTC CCAGCAGTGT TCATCAGAAG ATGAAGACAT TTTTGAGGAG
    ACTGCCCAGG TGAGCCCCCC GAGAGGCAAA GAAAAGAGGC AGTGGAGGGC TCGGATGCCC
    TCGCTCAGCG TCCAGCCCGT CAGCAACGCG GACTGGGTGT GGCTGGTCAA GAGGCTTCAC
    AAGCTGTGCA TGGAGCTGTG TAACAACTAC ATCCAGATGC ACCTGGACCT GGAAAACAGT
    ATGGAGGAGG CCCCAATCTT CAAGAGTGAC CCTTTCTTCA TCCTTCCCTC CTTCCAGTCG
    GAGTCCTCCA CCCCTTCCAC CGGGGGCTTC TCAGGGAAAG ACACGCCTTC CGAGGATGAC
    CGCAGTCAGA CCCGGGATCA TGCGGGCGAG CCTCTGAGCC TGAGGGGGAC CAGCGGGGAG
    CTGCTGCTGC TGCCCCCGAG CCCCAAGGTG GAGAAGAAGG ATCCCAGCCG CAAGAAGGAG
    TGGTGGGAGA ACGCGGGCAA CAAAATCTAT ACCATGGCAG CCGACAAGAC CATCTCAAAG
    CTGATGACCG AGTACAAGAG GAGGAAACAG CAGCACAACC TGCCTGTCTT CCCCAAAGAG
    ATCAAGGTGG AGAAGAAGGG AGAGGTGCTG GGCGCCAGGG GCCAAGGCTC CCCACTGCTC
    CAGCGACCCC AGCACCTGAT GGACCAGGGG CAGATGCGGC ACTCTTTCAG CGCCGGCCCT
    GAGCTGCTGC GCCAGGAGAA GAGGCCCCGC TCCGGCTCCA CCGGCAGCTC CCGGAGTGTC
    TCCGCGAGGG ACGCAGAAGC CCAGATCCAG GCATGGACCA ACATGGTGCT AACAGTCCTC
    AATCAGATTC AGATCCTCCC AGACCAGACC TTCACAGCCC TCCAGCCAGC AGTGTTTCCG
    TGTATCAGCC AGCTCACCTG TCACGTGACT GACATCAGAG TTCGCCAGGC TGTGAGAGAA
    TGGCTCGGCA GAGTGGGCCG TGTCTATGAC ATCATTATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328372.1
    CDS1094..7375
    Translation

    Target ORF information:

    RefSeq Version XM_015472886.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAAACGG ACTCTGATGA GAAGCAGCTG CTCAATCAGA TCCTGAACGC TGTGAAAGTG 
    ACGCCTTCCC TCCACGAAGA CCTGCAGGTG GAAGTGATGA AGGTTTTACT CTGCATAACC
    TACACTCCAA CCTTTGACCT GAATGGGAGT GCAGTGCTGA AGATCGCGGA GGTGTGCATT
    GAAACATACA TATGTAGCTG TCACCAGCGT AGTATAAACA CTGCTGTGCG GGCAACTCTC
    AGTCAAATGC TGAGTGACTT GACTTTACAG TTACGACAAA GGCAGGAGAA CACGATAATT
    GAAAACCCGG ATGTCCCCCA GGAATTCAGG AGTCAAGGGT CAACAGTAGA GTCCCTCTGT
    GATGACCTTG TCTCCGTGCT CACTGTTCTG TGTGAGAAGC TCCAAGCTGC CATCAATGAC
    AGCCAGCAGC TGCAGCTCCT GTACCTGGAG TGCATCCTGT CCGTGCTCAG CAGCTCCTCC
    TCCTCCATGC AGCTGCACCG AGGCTTCACA GACCTCATCT GGAAGAACCT TTGCCCCGCT
    CTCATCGTGA TCCTGGGGAA TCCGATTCAT GATAAAACCA TCACCTCTGC ACACAGCAGC
    AGCACGAGTA CCAGCATCGA GTCAGACTCT GTGTCTCCTG GGGTTTCCGA CCACGGCCGG
    GGTTCGGGCT GCTCCTCCAC AGCGCCAGCC CTGAGTGGGC CAGTGGCCCG GACCATCTAC
    TACATCGCGG CTGAGCTGGT GCGGCTGGTG GGGTCGGTGG ACTCCATGAA GCCCGTGTTG
    CAGTCCCTCT ACCACCGTGT GCTGCTCTAC CCCCCACCCC AGCACCGTGT GGAAGCCATC
    AAGATCATGA AAGAGCTACT TGGGAGCCCA AAGCGTCTCT GTGATTTGGC AGGACCCAGC
    TCCACTGAAC CTGACTCGAG AAAAAGATCA ATTTCAAAAA GAAAGTCTCA TCTGGATCTT
    CTCAAACTCA TCATGGACGG CATGACCGAG GCGTGTATCA AGGGCGGCAT CGAGGCCTGC
    TACGCCGCCG TGTCCTGCGT CTGCACCTTG CTGGGAGCCT TGGAGGAGCT CAGCCAGGGC
    AGGGGCTTGA GCGAAAGTCA AGTCCAGCTG CTGCTGCTGC GCCTGGAGGA GCTGAAGGAC
    GGGGCCGAGT CCAGCCGCGA CTCCATGGAG ATCAATGAGG CCGACTTCCG CTGGCAGCGG
    CGAGTCCTGT CCTCGGAACA CACACCCTGG GAGTTGGGGA ATGAGCGGAG CCTTGACATC
    AGCATCAGCG TTACCACGGA CACGGGCCAG ACCACCTTGG AGGGAGAGTT GGGTCAGACC
    ACACCCGAGG ACCACTCGGA AAACCCCAAG AATGGCCTCA AGTCACCAGC CGTCCAGGAG
    GGCAAGGAGA CTCTGAGTAA AGTGTCAGAA CCAGAAGCTG TGGACCAGCC AGACGTGGTT
    CAAAGGAGCC ACACGGTTGC CTACCCCGAT ATAACCAACT TCCTGTCCGT CGACTGCAGG
    ATGAGGTCCT ACGGATCCAG ATACAGTGAG AGCAACTTCA GCGTGGACGA CCAAGACCTC
    TCTCGGACAG AATTCGACTC CTGTGACCAG TACTCCATGG CGGCTGAAAA GGACTCCGGG
    AGATCGGACG TGTCGGACAT TGGATCCGAC AACTGTTCTT TAGCTGATGA GGAGCAGACC
    CCCCGGGACT GCCTGGGCCA CAGGTCCCTG CGGACTGCAG CTCTGTCTCT CAAGCTGCTG
    AAGAACCAGG AGGCTGACCA GCACAGCGCC CGGCTGTTCG TGCAGTCGCT GGAGGGTCTC
    CTGCCGCGCC TCCTGGCCCT GCCCAGTGTG GAAGAGGTGG ACTCAGCCCT CCAGAACTTT
    GTCTCTACCT TCTGCTCAGG TATGATGCAT TCACCAGGGT TTGATGGGAA CAGCAGCCTG
    GGCCTGCAGA TGCTGATGAA CGCGGACAGC CTGTACTCGG CGGCGCACTG CGCGCTGCTG
    CTCAACCTGA AGCTCTCCCA CGGTGACTAC TACCGCAAGC GGCCCACCCT AGCACCCAGC
    ACCATGAAAG AGTTCATGAA GCAGGTCCAG ACCAGCGGGG TGCTGATGGT CTTCTCCCAG
    GCCTGGATTG AGGAACTTTA CCACCAGGTT CTTGACAGAA ACATGCTCGG AGAAGCCGGC
    TACTGGGGCA GCCCTGAAGA CAACAGCCTC CCCCTCATCA CCATGCTGAC GGATATCGAT
    GGCTTAGAGA GCAGTGCAAT TGGAGGGCAG CTGATGGCCT CAGCTTCGAT GGAGTCTCCT
    TTCACCCAGG GCAGGAGAAT TGATGACTCC ACGGTGGCAG GTGTGGCATT TGCTCGCTAT
    ATTCTGATTG GCTGCTGGAA GAACTTGATC GACACTTTAT CAACCCCCCT GACGGGCCGA
    ATGGCCGGCA GCTCCAAAGG GCTGGCCTTC ATCTTGGGAG CTGAAGGCAT CAAAGAACAG
    AACCAAAAAG AACGCGATGC TATCTGCATG AGCCTCGACG GGCTACGGAA AGCCGCGCGT
    CTGAGCTGCG CTCTAGGAGT TGCAGCCAAC TGTGCTTCGG CCCTCGCCCA GATGGCAGCT
    GCCTCCTGTG TCCAGGAGGA GAAAGAGGAA AAGGAAACCC AGGAACCCAG TGATGCCATA
    GCACAAGTGA AACTAAAAGT GGAGCAGAAG CTGGAGCAGA TCGGGAAGCT GCAGGGGGTG
    TGGCTGCACA CAGCCCACGT CCTGTGCATG GACGCCATCC TCAGTGTCGG CCTGGAGATG
    GGCAGCCACA ACCCCGACTG CTGGCCACAC GTGTTCAGGG TGTGCGAGTA CGTGGGCACC
    TTGGAACACA CCCACTTCAG CGATGGCACC TCTCAGCCCC CGCTGACCAT CAGCCAGCCC
    CAGAAGGCCC CCAGAGGCGG ACTCCTTGGG GACCTGGAGA GTGAGGGCTC AGCCCAGGAG
    CAGAGCCTGG AGCAGGAGAG CTCCCTGAGC GCGGCCCCCG TCATCCAACC GCTCTCCATC
    CAGGAGCTCG TCCGAGAAGG CAGCCGAGGC CGGGCCTCTG ACTTCCGTGG GGGCAGCCTC
    ATGACAGGCA GCAGTGCCGC CAAGGTGGTG CTCACGCTCT CCACGCAGGC TGACAGGCTC
    TTTGAAGATG CTACAGATAA ACTGAACCTC ATGGCTCTGG GAGGTTTTCT TTACCAGCTG
    AAGAAAGCAT CGCAGTCCCA GCTTTTCCAT TCTGTTACGG AAACAGTTGA TTACTCGCTG
    GCAATGCCAG GAGAAGTGAA GTCTACCCAA GACCGCAAAA GTGCCCTCCA CCTGTTCCGC
    CTCGGGGACG CCATGCTAAG GATCGTGAGG AGCAAATCGA GGCCCCTGCT CCACGTGATG
    CGCTGCTGGA GCCTCGTGGC CCCACACCTG GTAGAGGCTG CCTGCCATAA GGAAAGACAC
    GTGTCTCAGA AGGCGGTTTC CTTCATCCAT GACATACTGA CGGAGGTTCT CACGGACTGG
    AATGAGCCAC CTCATTTCCA CTTTAACGAA GCTCTCTTCC GACCGTTCGA ACGTATCATG
    CAGCTGGAGT TGTGTGACGA GGACGTCCAA GACCAGGTTG TCACGTCCAT CGGGGAGCTG
    GTTGAAGTGT GCTCTGCCCA GATCCAGTCA GGCTGGAGGC CCCTGTTCAG CGCCCTGGAA
    ACGGTGCACA GCGGGAACAA GTCCGAGGTG AAGGAGTACC TGGTGGGCGA CTATTCCATG
    GGAAAAGGCC AGGCTCCAGT GTTTGATGTA TTTGAAGCTT TTCTCAATAC CGACAACATC
    CAGGTCTTTG CTAATGCAGC CACTAGTTAC ATCATGTGCC TTATGAAGTT TGTCAAAGGA
    CTGGGGGAGG TGGACTGTAA GGAGATTGGA GACTGTGTCC CAGGACCCGG AGCCGCGTCC
    CCAGACCTGT GCCTCCCCGC CCTGGACTAC CTCCGGCGCT GTTCTCAGTT GTTGGCCAAA
    ATCTACAAAA TGCCCTTGAA GCCAATATTC CTTAGTGGGC GACTTGCTAG CTTGCCCCGA
    AGACTTCAGG AGCAGTCAGC GAGCAGTGAG GATGGAATTG AGTCAGTCTT GTCTGACTTT
    GATGACGACA CTGGTCTGAT CGAAGTTTGG ATCATCCTCT TGGAGCAGCT CACAGCAGCT
    GTGTCCAACT GTCCACGTCA GCACCAACCC CCCACTCTGG ATTTGCTCTT TGAGCTGTTG
    AGAGAGGTTA CCAAAACACC TGGACCAGGG TTTGGCATCT ATGCAGTTGT TCACCTTCTC
    CTTCCTGTGA TGTCTCTTTG GCTTCGCCGT AGCCATAAAG ACCATTCTTA CTGGGACATT
    GCCTCGGCTA ACTTCAAGCA TGCCATTGGT CTGTCCTGTG AGCTGGTGGT GGAGCACATT
    CAGAGCTTTA TCCACTCAGA CATCAGGTAT GAGAGCATGA TCAACACCAT GCTGAAGGAC
    CTCTTTGAGC TACTGGTAGC ATGTGTAGCC AAGCCCACAG AAACCATCTC CCGAGTAGGC
    TGCTCCTGTA TTAGGTACGT CCTCGTGACA GCGGGCCCCG TGTTCACGGA GGAGATGTGG
    AGGCTGGCCT GCTGTGCCCT GCAGGACGCC TTCTCGGCCA CACTCAAGCC GGTGAAGGAC
    CTGCTGGGCT GCTTCCACAG TGGCACCGAG AGCTTCAGCG GCGAAGGCTG CCAGGTGAGG
    GTGGCCGCCC CGTCCTCCTC CCCGAGTGCC GAGGCCGAGT ACTGGCGCAT CCGAGCCATG
    GCTCAGCAGG TATTTATGTT GGATACCCAG TGCTCACCGA AGACTCCAAA CAACTTCGAT
    CATGCTCAGT CCTGTCAGCT CATTATTGAA CTGCCTCCTG ATGAGAAGCC AAATGGGCAT
    ACCAAGAAAA GTGTTTCTTT CAGGGAAATT GTGGTGAGTT TACTATCCCA TCAAGTATTA
    CTCCAGAACT TATATGATAT CTTATTAGAA GAGTTTGTCA AAGGCCCCTC TCCTGGGGAG
    GAGAAGATCC AAGGGCCAGA AACCAAGCTG GCTGGCTTCC TCAGATACAT CTCCATGCAG
    AACCTGGCCG TCATCTTTGA CCTTCTGCTG GACTCCTACA GGACGGCCAG AGAGTTTGAC
    ACCAGCCCAG GGCTGAAGTG CCTGCTGAAG AAAGTGTCTG GCATAGGGGG CGCTGCCAAC
    CTCTACCGCC AGTCTGCCAT GAGTTTCAAC ATTTATTTCC ACGCACTGGT CTGTGCTGTT
    CTTAGCAACC AAGAAACCAT CACTGCTGAC CAAGTGAAGA AGGTCCTTTT TGAGGACGAT
    GAGAGGAGCA CAGATTCGTC CCAGCAGTGT TCATCAGAAG ATGAAGACAT TTTTGAGGAG
    ACTGCCCAGG TGAGCCCCCC GAGAGGCAAA GAAAAGAGGC AGTGGAGGGC TCGGATGCCC
    TCGCTCAGCG TCCAGCCCGT CAGCAACGCG GACTGGGTGT GGCTGGTCAA GAGGCTTCAC
    AAGCTGTGCA TGGAGCTGTG TAACAACTAC ATCCAGATGC ACCTGGACCT GGAAAACAGT
    ATGGAGGAGG CCCCAATCTT CAAGAGTGAC CCTTTCTTCA TCCTTCCCTC CTTCCAGTCG
    GAGTCCTCCA CCCCTTCCAC CGGGGGCTTC TCAGGGAAAG ACACGCCTTC CGAGGATGAC
    CGCAGTCAGA CCCGGGATCA TGCGGGCGAG CCTCTGAGCC TGAGGGGGAC CAGCGGGGAG
    CTGCTGCTGC TGCCCCCGAG CCCCAAGGTG GAGAAGAAGG ATCCCAGCCG CAAGAAGGAG
    TGGTGGGAGA ACGCGGGCAA CAAAATCTAT ACCATGGCAG CCGACAAGAC CATCTCAAAG
    CTGATGACCG AGTACAAGAG GAGGAAACAG CAGCACAACC TGCCTGTCTT CCCCAAAGAG
    ATCAAGGTGG AGAAGAAGGG AGAGGTGCTG GGCGCCAGGG GCCAAGGCTC CCCACTGCTC
    CAGCGACCCC AGCACCTGAT GGACCAGGGG CAGATGCGGC ACTCTTTCAG CGCCGGCCCT
    GAGCTGCTGC GCCAGGAGAA GAGGCCCCGC TCCGGCTCCA CCGGCAGCTC CCGGAGTGTC
    TCCGCGAGGG ACGCAGAAGC CCAGATCCAG GCATGGACCA ACATGGTGCT AACAGTCCTC
    AATCAGATTC AGATCCTCCC AGACCAGACC TTCACAGCCC TCCAGCCAGC AGTGTTTCCG
    TGTATCAGCC AGCTCACCTG TCACGTGACT GACATCAGAG TTCGCCAGGC TGTGAGAGAA
    TGGCTCGGCA GAGTGGGCCG TGTCTATGAC ATCATTATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22720
    Clone ID Related Accession (Same CDS sequence) XM_015464837.1 , XM_002690274.4 , XM_002690274.5
    Accession Version XM_015464837.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645711.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    GTGTGCATTG AAACATACAT ATGTAGCTGT CACCAGCGTA GTATAAACAC TGCTGTGCGG
    GCAACTCTCA GTCAAATGCT GAGTGACTTG ACTTTACAGT TACGACAAAG GCAGGAGAAC
    ACGATAATTG AAAACCCGGA TGTCCCCCAG GAATTCAGGA GTCAAGGGTC AACAGTAGAG
    TCCCTCTGTG ATGACCTTGT CTCCGTGCTC ACTGTTCTGT GTGAGAAGCT CCAAGCTGCC
    ATCAATGACA GCCAGCAGCT GCAGCTCCTG TACCTGGAGT GCATCCTGTC CGTGCTCAGC
    AGCTCCTCCT CCTCCATGCA GCTGCACCGA GGCTTCACAG ACCTCATCTG GAAGAACCTT
    TGCCCCGCTC TCATCGTGAT CCTGGGGAAT CCGATTCATG ATAAAACCAT CACCTCTGCA
    CACAGCAGCA GCACGAGTAC CAGCATCGAG TCAGACTCTG TGTCTCCTGG GGTTTCCGAC
    CACGGCCGGG GTTCGGGCTG CTCCTCCACA GCGCCAGCCC TGAGTGGGCC AGTGGCCCGG
    ACCATCTACT ACATCGCGGC TGAGCTGGTG CGGCTGGTGG GGTCGGTGGA CTCCATGAAG
    CCCGTGTTGC AGTCCCTCTA CCACCGTGTG CTGCTCTACC CCCCACCCCA GCACCGTGTG
    GAAGCCATCA AGATCATGAA AGAGCTACTT GGGAGCCCAA AGCGTCTCTG TGATTTGGCA
    GGACCCAGCT CCACTGAACC TGACTCGAGA AAAAGATCAA TTTCAAAAAG AAAGTCTCAT
    CTGGATCTTC TCAAACTCAT CATGGACGGC ATGACCGAGG CGTGTATCAA GGGCGGCATC
    GAGGCCTGCT ACGCCGCCGT GTCCTGCGTC TGCACCTTGC TGGGAGCCTT GGAGGAGCTC
    AGCCAGGGCA GGGGCTTGAG CGAAAGTCAA GTCCAGCTGC TGCTGCTGCG CCTGGAGGAG
    CTGAAGGACG GGGCCGAGTC CAGCCGCGAC TCCATGGAGA TCAATGAGGC CGACTTCCGC
    TGGCAGCGGC GAGTCCTGTC CTCGGAACAC ACACCCTGGG AGTTGGGGAA TGAGCGGAGC
    CTTGACATCA GCATCAGCGT TACCACGGAC ACGGGCCAGA CCACCTTGGA GGGAGAGTTG
    GGTCAGACCA CACCCGAGGA CCACTCGGAA AACCCCAAGA ATGGCCTCAA GTCACCAGCC
    GTCCAGGAGG GCAAGGAGAC TCTGAGTAAA GTGTCAGAAC CAGAAGCTGT GGACCAGCCA
    GACGTGGTTC AAAGGAGCCA CACGGTTGCC TACCCCGATA TAACCAACTT CCTGTCCGTC
    GACTGCAGGA TGAGGTCCTA CGGATCCAGA TACAGTGAGA GCAACTTCAG CGTGGACGAC
    CAAGACCTCT CTCGGACAGA ATTCGACTCC TGTGACCAGT ACTCCATGGC GGCTGAAAAG
    GACTCCGGGA GATCGGACGT GTCGGACATT GGATCCGACA ACTGTTCTTT AGCTGATGAG
    GAGCAGACCC CCCGGGACTG CCTGGGCCAC AGGTCCCTGC GGACTGCAGC TCTGTCTCTC
    AAGCTGCTGA AGAACCAGGA GGCTGACCAG CACAGCGCCC GGCTGTTCGT GCAGTCGCTG
    GAGGGTCTCC TGCCGCGCCT CCTGGCCCTG CCCAGTGTGG AAGAGGTGGA CTCAGCCCTC
    CAGAACTTTG TCTCTACCTT CTGCTCAGGT ATGATGCATT CACCAGGGTT TGATGGGAAC
    AGCAGCCTGG GCCTGCAGAT GCTGATGAAC GCGGACAGCC TGTACTCGGC GGCGCACTGC
    GCGCTGCTGC TCAACCTGAA GCTCTCCCAC GGTGACTACT ACCGCAAGCG GCCCACCCTA
    GCACCCAGCA CCATGAAAGA GTTCATGAAG CAGGTCCAGA CCAGCGGGGT GCTGATGGTC
    TTCTCCCAGG CCTGGATTGA GGAACTTTAC CACCAGGTTC TTGACAGAAA CATGCTCGGA
    GAAGCCGGCT ACTGGGGCAG CCCTGAAGAC AACAGCCTCC CCCTCATCAC CATGCTGACG
    GATATCGATG GCTTAGAGAG CAGTGCAATT GGAGGGCAGC TGATGGCCTC AGCTTCGATG
    GAGTCTCCTT TCACCCAGGG CAGGAGAATT GATGACTCCA CGGTGGCAGG TGTGGCATTT
    GCTCGCTATA TTCTGATTGG CTGCTGGAAG AACTTGATCG ACACTTTATC AACCCCCCTG
    ACGGGCCGAA TGGCCGGCAG CTCCAAAGGG CTGGCCTTCA TCTTGGGAGC TGAAGGCATC
    AAAGAACAGA ACCAAAAAGA ACGCGATGCT ATCTGCATGA GCCTCGACGG GCTACGGAAA
    GCCGCGCGTC TGAGCTGCGC TCTAGGAGTT GCAGCCAACT GTGCTTCGGC CCTCGCCCAG
    ATGGCAGCTG CCTCCTGTGT CCAGGAGGAG AAAGAGGAAA AGGAAACCCA GGAACCCAGT
    GATGCCATAG CACAAGTGAA ACTAAAAGTG GAGCAGAAGC TGGAGCAGAT CGGGAAGCTG
    CAGGGGGTGT GGCTGCACAC AGCCCACGTC CTGTGCATGG ACGCCATCCT CAGTGTCGGC
    CTGGAGATGG GCAGCCACAA CCCCGACTGC TGGCCACACG TGTTCAGGGT GTGCGAGTAC
    GTGGGCACCT TGGAACACAC CCACTTCAGC GATGGCACCT CTCAGCCCCC GCTGACCATC
    AGCCAGCCCC AGAAGGCCCC CAGAGGCGGA CTCCTTGGGG ACCTGGAGAG TGAGGGCTCA
    GCCCAGGAGC AGAGCCTGGA GCAGGAGAGC TCCCTGAGCG CGGCCCCCGT CATCCAACCG
    CTCTCCATCC AGGAGCTCGT CCGAGAAGGC AGCCGAGGCC GGGCCTCTGA CTTCCGTGGG
    GGCAGCCTCA TGACAGGCAG CAGTGCCGCC AAGGTGGTGC TCACGCTCTC CACGCAGGCT
    GACAGGCTCT TTGAAGATGC TACAGATAAA CTGAACCTCA TGGCTCTGGG AGGTTTTCTT
    TACCAGCTGA AGAAAGCATC GCAGTCCCAG CTTTTCCATT CTGTTACGGA AACAGTTGAT
    TACTCGCTGG CAATGCCAGG AGAAGTGAAG TCTACCCAAG ACCGCAAAAG TGCCCTCCAC
    CTGTTCCGCC TCGGGGACGC CATGCTAAGG ATCGTGAGGA GCAAATCGAG GCCCCTGCTC
    CACGTGATGC GCTGCTGGAG CCTCGTGGCC CCACACCTGG TAGAGGCTGC CTGCCATAAG
    GAAAGACACG TGTCTCAGAA GGCGGTTTCC TTCATCCATG ACATACTGAC GGAGGTTCTC
    ACGGACTGGA ATGAGCCACC TCATTTCCAC TTTAACGAAG CTCTCTTCCG ACCGTTCGAA
    CGTATCATGC AGCTGGAGTT GTGTGACGAG GACGTCCAAG ACCAGGTTGT CACGTCCATC
    GGGGAGCTGG TTGAAGTGTG CTCTGCCCAG ATCCAGTCAG GCTGGAGGCC CCTGTTCAGC
    GCCCTGGAAA CGGTGCACAG CGGGAACAAG TCCGAGGTGA AGGAGTACCT GGTGGGCGAC
    TATTCCATGG GAAAAGGCCA GGCTCCAGTG TTTGATGTAT TTGAAGCTTT TCTCAATACC
    GACAACATCC AGGTCTTTGC TAATGCAGCC ACTAGTTACA TCATGTGCCT TATGAAGTTT
    GTCAAAGGAC TGGGGGAGGT GGACTGTAAG GAGATTGGAG ACTGTGTCCC AGGACCCGGA
    GCCGCGTCCC CAGACCTGTG CCTCCCCGCC CTGGACTACC TCCGGCGCTG TTCTCAGTTG
    TTGGCCAAAA TCTACAAAAT GCCCTTGAAG CCAATATTCC TTAGTGGGCG ACTTGCTAGC
    TTGCCCCGAA GACTTCAGGA GCAGTCAGCG AGCAGTGAGG ATGGAATTGA GTCAGTCTTG
    TCTGACTTTG ATGACGACAC TGGTCTGATC GAAGTTTGGA TCATCCTCTT GGAGCAGCTC
    ACAGCAGCTG TGTCCAACTG TCCACGTCAG CACCAACCCC CCACTCTGGA TTTGCTCTTT
    GAGCTGTTGA GAGAGGTTAC CAAAACACCT GGACCAGGGT TTGGCATCTA TGCAGTTGTT
    CACCTTCTCC TTCCTGTGAT GTCTCTTTGG CTTCGCCGTA GCCATAAAGA CCATTCTTAC
    TGGGACATTG CCTCGGCTAA CTTCAAGCAT GCCATTGGTC TGTCCTGTGA GCTGGTGGTG
    GAGCACATTC AGAGCTTTAT CCACTCAGAC ATCAGGTATG AGAGCATGAT CAACACCATG
    CTGAAGGACC TCTTTGAGCT ACTGGTAGCA TGTGTAGCCA AGCCCACAGA AACCATCTCC
    CGAGTAGGCT GCTCCTGTAT TAGGTACGTC CTCGTGACAG CGGGCCCCGT GTTCACGGAG
    GAGATGTGGA GGCTGGCCTG CTGTGCCCTG CAGGACGCCT TCTCGGCCAC ACTCAAGCCG
    GTGAAGGACC TGCTGGGCTG CTTCCACAGT GGCACCGAGA GCTTCAGCGG CGAAGGCTGC
    CAGGTGAGGG TGGCCGCCCC GTCCTCCTCC CCGAGTGCCG AGGCCGAGTA CTGGCGCATC
    CGAGCCATGG CTCAGCAGGT ATTTATGTTG GATACCCAGT GCTCACCGAA GACTCCAAAC
    AACTTCGATC ATGCTCAGTC CTGTCAGCTC ATTATTGAAC TGCCTCCTGA TGAGAAGCCA
    AATGGGCATA CCAAGAAAAG TGTTTCTTTC AGGGAAATTG TGGTGAGTTT ACTATCCCAT
    CAAGTATTAC TCCAGAACTT ATATGATATC TTATTAGAAG AGTTTGTCAA AGGCCCCTCT
    CCTGGGGAGG AGAAGATCCA AGGGCCAGAA ACCAAGCTGG CTGGCTTCCT CAGATACATC
    TCCATGCAGA ACCTGGCCGT CATCTTTGAC CTTCTGCTGG ACTCCTACAG GACGGCCAGA
    GAGTTTGACA CCAGCCCAGG GCTGAAGTGC CTGCTGAAGA AAGTGTCTGG CATAGGGGGC
    GCTGCCAACC TCTACCGCCA GTCTGCCATG AGTTTCAACA TTTATTTCCA CGCACTGGTC
    TGTGCTGTTC TTAGCAACCA AGAAACCATC ACTGCTGACC AAGTGAAGAA GGTCCTTTTT
    GAGGACGATG AGAGGAGCAC AGATTCGTCC CAGCAGTGTT CATCAGAAGA TGAAGACATT
    TTTGAGGAGA CTGCCCAGGT GAGCCCCCCG AGAGGCAAAG AAAAGAGGCA GTGGAGGGCT
    CGGATGCCCT CGCTCAGCGT CCAGCCCGTC AGCAACGCGG ACTGGGTGTG GCTGGTCAAG
    AGGCTTCACA AGCTGTGCAT GGAGCTGTGT AACAACTACA TCCAGATGCA CCTGGACCTG
    GAAAACAGTA TGGAGGAGGC CCCAATCTTC AAGAGTGACC CTTTCTTCAT CCTTCCCTCC
    TTCCAGTCGG AGTCCTCCAC CCCTTCCACC GGGGGCTTCT CAGGGAAAGA CACGCCTTCC
    GAGGATGACC GCAGTCAGAC CCGGGATCAT GCGGGCGAGC CTCTGAGCCT GAGGGGGACC
    AGCGGGGAGC TGCTGCTGCT GCCCCCGAGC CCCAAGGTGG AGAAGAAGGA TCCCAGCCGC
    AAGAAGGAGT GGTGGGAGAA CGCGGGCAAC AAAATCTATA CCATGGCAGC CGACAAGACC
    ATCTCAAAGC TGATGACCGA GTACAAGAGG AGGAAACAGC AGCACAACCT GCCTGTCTTC
    CCCAAAGAGA TCAAGGTGGA GAAGAAGGGA GAGGTGCTGG GCGCCAGGGG CCAAGGCTCC
    CCACTGCTCC AGCGACCCCA GCACCTGATG GACCAGGGGC AGATGCGGCA CTCTTTCAGC
    GCCGGCCCTG AGCTGCTGCG CCAGGAGAAG AGGCCCCGCT CCGGCTCCAC CGGCAGCTCC
    CGGAGTGTCT CCGCGAGGGA CGCAGAAGCC CAGATCCAGG CATGGACCAA CATGGTGCTA
    ACAGTCCTCA ATCAGATTCA GATCCTCCCA GACCAGACCT TCACAGCCCT CCAGCCAGCA
    GTGTTTCCGT GTATCAGCCA GCTCACCTGT CACGTGACTG ACATCAGAGT TCGCCAGGCT
    GTGAGAGAAT GGCTCGGCAG AGTGGGCCGT GTCTATGACA TCATTATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320323.1
    CDS181..6711
    Translation

    Target ORF information:

    RefSeq Version XM_015464837.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    GTGTGCATTG AAACATACAT ATGTAGCTGT CACCAGCGTA GTATAAACAC TGCTGTGCGG
    GCAACTCTCA GTCAAATGCT GAGTGACTTG ACTTTACAGT TACGACAAAG GCAGGAGAAC
    ACGATAATTG AAAACCCGGA TGTCCCCCAG GAATTCAGGA GTCAAGGGTC AACAGTAGAG
    TCCCTCTGTG ATGACCTTGT CTCCGTGCTC ACTGTTCTGT GTGAGAAGCT CCAAGCTGCC
    ATCAATGACA GCCAGCAGCT GCAGCTCCTG TACCTGGAGT GCATCCTGTC CGTGCTCAGC
    AGCTCCTCCT CCTCCATGCA GCTGCACCGA GGCTTCACAG ACCTCATCTG GAAGAACCTT
    TGCCCCGCTC TCATCGTGAT CCTGGGGAAT CCGATTCATG ATAAAACCAT CACCTCTGCA
    CACAGCAGCA GCACGAGTAC CAGCATCGAG TCAGACTCTG TGTCTCCTGG GGTTTCCGAC
    CACGGCCGGG GTTCGGGCTG CTCCTCCACA GCGCCAGCCC TGAGTGGGCC AGTGGCCCGG
    ACCATCTACT ACATCGCGGC TGAGCTGGTG CGGCTGGTGG GGTCGGTGGA CTCCATGAAG
    CCCGTGTTGC AGTCCCTCTA CCACCGTGTG CTGCTCTACC CCCCACCCCA GCACCGTGTG
    GAAGCCATCA AGATCATGAA AGAGCTACTT GGGAGCCCAA AGCGTCTCTG TGATTTGGCA
    GGACCCAGCT CCACTGAACC TGACTCGAGA AAAAGATCAA TTTCAAAAAG AAAGTCTCAT
    CTGGATCTTC TCAAACTCAT CATGGACGGC ATGACCGAGG CGTGTATCAA GGGCGGCATC
    GAGGCCTGCT ACGCCGCCGT GTCCTGCGTC TGCACCTTGC TGGGAGCCTT GGAGGAGCTC
    AGCCAGGGCA GGGGCTTGAG CGAAAGTCAA GTCCAGCTGC TGCTGCTGCG CCTGGAGGAG
    CTGAAGGACG GGGCCGAGTC CAGCCGCGAC TCCATGGAGA TCAATGAGGC CGACTTCCGC
    TGGCAGCGGC GAGTCCTGTC CTCGGAACAC ACACCCTGGG AGTTGGGGAA TGAGCGGAGC
    CTTGACATCA GCATCAGCGT TACCACGGAC ACGGGCCAGA CCACCTTGGA GGGAGAGTTG
    GGTCAGACCA CACCCGAGGA CCACTCGGAA AACCCCAAGA ATGGCCTCAA GTCACCAGCC
    GTCCAGGAGG GCAAGGAGAC TCTGAGTAAA GTGTCAGAAC CAGAAGCTGT GGACCAGCCA
    GACGTGGTTC AAAGGAGCCA CACGGTTGCC TACCCCGATA TAACCAACTT CCTGTCCGTC
    GACTGCAGGA TGAGGTCCTA CGGATCCAGA TACAGTGAGA GCAACTTCAG CGTGGACGAC
    CAAGACCTCT CTCGGACAGA ATTCGACTCC TGTGACCAGT ACTCCATGGC GGCTGAAAAG
    GACTCCGGGA GATCGGACGT GTCGGACATT GGATCCGACA ACTGTTCTTT AGCTGATGAG
    GAGCAGACCC CCCGGGACTG CCTGGGCCAC AGGTCCCTGC GGACTGCAGC TCTGTCTCTC
    AAGCTGCTGA AGAACCAGGA GGCTGACCAG CACAGCGCCC GGCTGTTCGT GCAGTCGCTG
    GAGGGTCTCC TGCCGCGCCT CCTGGCCCTG CCCAGTGTGG AAGAGGTGGA CTCAGCCCTC
    CAGAACTTTG TCTCTACCTT CTGCTCAGGT ATGATGCATT CACCAGGGTT TGATGGGAAC
    AGCAGCCTGG GCCTGCAGAT GCTGATGAAC GCGGACAGCC TGTACTCGGC GGCGCACTGC
    GCGCTGCTGC TCAACCTGAA GCTCTCCCAC GGTGACTACT ACCGCAAGCG GCCCACCCTA
    GCACCCAGCA CCATGAAAGA GTTCATGAAG CAGGTCCAGA CCAGCGGGGT GCTGATGGTC
    TTCTCCCAGG CCTGGATTGA GGAACTTTAC CACCAGGTTC TTGACAGAAA CATGCTCGGA
    GAAGCCGGCT ACTGGGGCAG CCCTGAAGAC AACAGCCTCC CCCTCATCAC CATGCTGACG
    GATATCGATG GCTTAGAGAG CAGTGCAATT GGAGGGCAGC TGATGGCCTC AGCTTCGATG
    GAGTCTCCTT TCACCCAGGG CAGGAGAATT GATGACTCCA CGGTGGCAGG TGTGGCATTT
    GCTCGCTATA TTCTGATTGG CTGCTGGAAG AACTTGATCG ACACTTTATC AACCCCCCTG
    ACGGGCCGAA TGGCCGGCAG CTCCAAAGGG CTGGCCTTCA TCTTGGGAGC TGAAGGCATC
    AAAGAACAGA ACCAAAAAGA ACGCGATGCT ATCTGCATGA GCCTCGACGG GCTACGGAAA
    GCCGCGCGTC TGAGCTGCGC TCTAGGAGTT GCAGCCAACT GTGCTTCGGC CCTCGCCCAG
    ATGGCAGCTG CCTCCTGTGT CCAGGAGGAG AAAGAGGAAA AGGAAACCCA GGAACCCAGT
    GATGCCATAG CACAAGTGAA ACTAAAAGTG GAGCAGAAGC TGGAGCAGAT CGGGAAGCTG
    CAGGGGGTGT GGCTGCACAC AGCCCACGTC CTGTGCATGG ACGCCATCCT CAGTGTCGGC
    CTGGAGATGG GCAGCCACAA CCCCGACTGC TGGCCACACG TGTTCAGGGT GTGCGAGTAC
    GTGGGCACCT TGGAACACAC CCACTTCAGC GATGGCACCT CTCAGCCCCC GCTGACCATC
    AGCCAGCCCC AGAAGGCCCC CAGAGGCGGA CTCCTTGGGG ACCTGGAGAG TGAGGGCTCA
    GCCCAGGAGC AGAGCCTGGA GCAGGAGAGC TCCCTGAGCG CGGCCCCCGT CATCCAACCG
    CTCTCCATCC AGGAGCTCGT CCGAGAAGGC AGCCGAGGCC GGGCCTCTGA CTTCCGTGGG
    GGCAGCCTCA TGACAGGCAG CAGTGCCGCC AAGGTGGTGC TCACGCTCTC CACGCAGGCT
    GACAGGCTCT TTGAAGATGC TACAGATAAA CTGAACCTCA TGGCTCTGGG AGGTTTTCTT
    TACCAGCTGA AGAAAGCATC GCAGTCCCAG CTTTTCCATT CTGTTACGGA AACAGTTGAT
    TACTCGCTGG CAATGCCAGG AGAAGTGAAG TCTACCCAAG ACCGCAAAAG TGCCCTCCAC
    CTGTTCCGCC TCGGGGACGC CATGCTAAGG ATCGTGAGGA GCAAATCGAG GCCCCTGCTC
    CACGTGATGC GCTGCTGGAG CCTCGTGGCC CCACACCTGG TAGAGGCTGC CTGCCATAAG
    GAAAGACACG TGTCTCAGAA GGCGGTTTCC TTCATCCATG ACATACTGAC GGAGGTTCTC
    ACGGACTGGA ATGAGCCACC TCATTTCCAC TTTAACGAAG CTCTCTTCCG ACCGTTCGAA
    CGTATCATGC AGCTGGAGTT GTGTGACGAG GACGTCCAAG ACCAGGTTGT CACGTCCATC
    GGGGAGCTGG TTGAAGTGTG CTCTGCCCAG ATCCAGTCAG GCTGGAGGCC CCTGTTCAGC
    GCCCTGGAAA CGGTGCACAG CGGGAACAAG TCCGAGGTGA AGGAGTACCT GGTGGGCGAC
    TATTCCATGG GAAAAGGCCA GGCTCCAGTG TTTGATGTAT TTGAAGCTTT TCTCAATACC
    GACAACATCC AGGTCTTTGC TAATGCAGCC ACTAGTTACA TCATGTGCCT TATGAAGTTT
    GTCAAAGGAC TGGGGGAGGT GGACTGTAAG GAGATTGGAG ACTGTGTCCC AGGACCCGGA
    GCCGCGTCCC CAGACCTGTG CCTCCCCGCC CTGGACTACC TCCGGCGCTG TTCTCAGTTG
    TTGGCCAAAA TCTACAAAAT GCCCTTGAAG CCAATATTCC TTAGTGGGCG ACTTGCTAGC
    TTGCCCCGAA GACTTCAGGA GCAGTCAGCG AGCAGTGAGG ATGGAATTGA GTCAGTCTTG
    TCTGACTTTG ATGACGACAC TGGTCTGATC GAAGTTTGGA TCATCCTCTT GGAGCAGCTC
    ACAGCAGCTG TGTCCAACTG TCCACGTCAG CACCAACCCC CCACTCTGGA TTTGCTCTTT
    GAGCTGTTGA GAGAGGTTAC CAAAACACCT GGACCAGGGT TTGGCATCTA TGCAGTTGTT
    CACCTTCTCC TTCCTGTGAT GTCTCTTTGG CTTCGCCGTA GCCATAAAGA CCATTCTTAC
    TGGGACATTG CCTCGGCTAA CTTCAAGCAT GCCATTGGTC TGTCCTGTGA GCTGGTGGTG
    GAGCACATTC AGAGCTTTAT CCACTCAGAC ATCAGGTATG AGAGCATGAT CAACACCATG
    CTGAAGGACC TCTTTGAGCT ACTGGTAGCA TGTGTAGCCA AGCCCACAGA AACCATCTCC
    CGAGTAGGCT GCTCCTGTAT TAGGTACGTC CTCGTGACAG CGGGCCCCGT GTTCACGGAG
    GAGATGTGGA GGCTGGCCTG CTGTGCCCTG CAGGACGCCT TCTCGGCCAC ACTCAAGCCG
    GTGAAGGACC TGCTGGGCTG CTTCCACAGT GGCACCGAGA GCTTCAGCGG CGAAGGCTGC
    CAGGTGAGGG TGGCCGCCCC GTCCTCCTCC CCGAGTGCCG AGGCCGAGTA CTGGCGCATC
    CGAGCCATGG CTCAGCAGGT ATTTATGTTG GATACCCAGT GCTCACCGAA GACTCCAAAC
    AACTTCGATC ATGCTCAGTC CTGTCAGCTC ATTATTGAAC TGCCTCCTGA TGAGAAGCCA
    AATGGGCATA CCAAGAAAAG TGTTTCTTTC AGGGAAATTG TGGTGAGTTT ACTATCCCAT
    CAAGTATTAC TCCAGAACTT ATATGATATC TTATTAGAAG AGTTTGTCAA AGGCCCCTCT
    CCTGGGGAGG AGAAGATCCA AGGGCCAGAA ACCAAGCTGG CTGGCTTCCT CAGATACATC
    TCCATGCAGA ACCTGGCCGT CATCTTTGAC CTTCTGCTGG ACTCCTACAG GACGGCCAGA
    GAGTTTGACA CCAGCCCAGG GCTGAAGTGC CTGCTGAAGA AAGTGTCTGG CATAGGGGGC
    GCTGCCAACC TCTACCGCCA GTCTGCCATG AGTTTCAACA TTTATTTCCA CGCACTGGTC
    TGTGCTGTTC TTAGCAACCA AGAAACCATC ACTGCTGACC AAGTGAAGAA GGTCCTTTTT
    GAGGACGATG AGAGGAGCAC AGATTCGTCC CAGCAGTGTT CATCAGAAGA TGAAGACATT
    TTTGAGGAGA CTGCCCAGGT GAGCCCCCCG AGAGGCAAAG AAAAGAGGCA GTGGAGGGCT
    CGGATGCCCT CGCTCAGCGT CCAGCCCGTC AGCAACGCGG ACTGGGTGTG GCTGGTCAAG
    AGGCTTCACA AGCTGTGCAT GGAGCTGTGT AACAACTACA TCCAGATGCA CCTGGACCTG
    GAAAACAGTA TGGAGGAGGC CCCAATCTTC AAGAGTGACC CTTTCTTCAT CCTTCCCTCC
    TTCCAGTCGG AGTCCTCCAC CCCTTCCACC GGGGGCTTCT CAGGGAAAGA CACGCCTTCC
    GAGGATGACC GCAGTCAGAC CCGGGATCAT GCGGGCGAGC CTCTGAGCCT GAGGGGGACC
    AGCGGGGAGC TGCTGCTGCT GCCCCCGAGC CCCAAGGTGG AGAAGAAGGA TCCCAGCCGC
    AAGAAGGAGT GGTGGGAGAA CGCGGGCAAC AAAATCTATA CCATGGCAGC CGACAAGACC
    ATCTCAAAGC TGATGACCGA GTACAAGAGG AGGAAACAGC AGCACAACCT GCCTGTCTTC
    CCCAAAGAGA TCAAGGTGGA GAAGAAGGGA GAGGTGCTGG GCGCCAGGGG CCAAGGCTCC
    CCACTGCTCC AGCGACCCCA GCACCTGATG GACCAGGGGC AGATGCGGCA CTCTTTCAGC
    GCCGGCCCTG AGCTGCTGCG CCAGGAGAAG AGGCCCCGCT CCGGCTCCAC CGGCAGCTCC
    CGGAGTGTCT CCGCGAGGGA CGCAGAAGCC CAGATCCAGG CATGGACCAA CATGGTGCTA
    ACAGTCCTCA ATCAGATTCA GATCCTCCCA GACCAGACCT TCACAGCCCT CCAGCCAGCA
    GTGTTTCCGT GTATCAGCCA GCTCACCTGT CACGTGACTG ACATCAGAGT TCGCCAGGCT
    GTGAGAGAAT GGCTCGGCAG AGTGGGCCGT GTCTATGACA TCATTATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22720
    Clone ID Related Accession (Same CDS sequence) XM_015464837.1 , XM_002690274.4 , XM_002690274.5
    Accession Version XM_002690274.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104223.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690274.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    GTGTGCATTG AAACATACAT ATGTAGCTGT CACCAGCGTA GTATAAACAC TGCTGTGCGG
    GCAACTCTCA GTCAAATGCT GAGTGACTTG ACTTTACAGT TACGACAAAG GCAGGAGAAC
    ACGATAATTG AAAACCCGGA TGTCCCCCAG GAATTCAGGA GTCAAGGGTC AACAGTAGAG
    TCCCTCTGTG ATGACCTTGT CTCCGTGCTC ACTGTTCTGT GTGAGAAGCT CCAAGCTGCC
    ATCAATGACA GCCAGCAGCT GCAGCTCCTG TACCTGGAGT GCATCCTGTC CGTGCTCAGC
    AGCTCCTCCT CCTCCATGCA GCTGCACCGA GGCTTCACAG ACCTCATCTG GAAGAACCTT
    TGCCCCGCTC TCATCGTGAT CCTGGGGAAT CCGATTCATG ATAAAACCAT CACCTCTGCA
    CACAGCAGCA GCACGAGTAC CAGCATCGAG TCAGACTCTG TGTCTCCTGG GGTTTCCGAC
    CACGGCCGGG GTTCGGGCTG CTCCTCCACA GCGCCAGCCC TGAGTGGGCC AGTGGCCCGG
    ACCATCTACT ACATCGCGGC TGAGCTGGTG CGGCTGGTGG GGTCGGTGGA CTCCATGAAG
    CCCGTGTTGC AGTCCCTCTA CCACCGTGTG CTGCTCTACC CCCCACCCCA GCACCGTGTG
    GAAGCCATCA AGATCATGAA AGAGCTACTT GGGAGCCCAA AGCGTCTCTG TGATTTGGCA
    GGACCCAGCT CCACTGAACC TGACTCGAGA AAAAGATCAA TTTCAAAAAG AAAGTCTCAT
    CTGGATCTTC TCAAACTCAT CATGGACGGC ATGACCGAGG CGTGTATCAA GGGCGGCATC
    GAGGCCTGCT ACGCCGCCGT GTCCTGCGTC TGCACCTTGC TGGGAGCCTT GGAGGAGCTC
    AGCCAGGGCA GGGGCTTGAG CGAAAGTCAA GTCCAGCTGC TGCTGCTGCG CCTGGAGGAG
    CTGAAGGACG GGGCCGAGTC CAGCCGCGAC TCCATGGAGA TCAATGAGGC CGACTTCCGC
    TGGCAGCGGC GAGTCCTGTC CTCGGAACAC ACACCCTGGG AGTTGGGGAA TGAGCGGAGC
    CTTGACATCA GCATCAGCGT TACCACGGAC ACGGGCCAGA CCACCTTGGA GGGAGAGTTG
    GGTCAGACCA CACCCGAGGA CCACTCGGAA AACCCCAAGA ATGGCCTCAA GTCACCAGCC
    GTCCAGGAGG GCAAGGAGAC TCTGAGTAAA GTGTCAGAAC CAGAAGCTGT GGACCAGCCA
    GACGTGGTTC AAAGGAGCCA CACGGTTGCC TACCCCGATA TAACCAACTT CCTGTCCGTC
    GACTGCAGGA TGAGGTCCTA CGGATCCAGA TACAGTGAGA GCAACTTCAG CGTGGACGAC
    CAAGACCTCT CTCGGACAGA ATTCGACTCC TGTGACCAGT ACTCCATGGC GGCTGAAAAG
    GACTCCGGGA GATCGGACGT GTCGGACATT GGATCCGACA ACTGTTCTTT AGCTGATGAG
    GAGCAGACCC CCCGGGACTG CCTGGGCCAC AGGTCCCTGC GGACTGCAGC TCTGTCTCTC
    AAGCTGCTGA AGAACCAGGA GGCTGACCAG CACAGCGCCC GGCTGTTCGT GCAGTCGCTG
    GAGGGTCTCC TGCCGCGCCT CCTGGCCCTG CCCAGTGTGG AAGAGGTGGA CTCAGCCCTC
    CAGAACTTTG TCTCTACCTT CTGCTCAGGT ATGATGCATT CACCAGGGTT TGATGGGAAC
    AGCAGCCTGG GCCTGCAGAT GCTGATGAAC GCGGACAGCC TGTACTCGGC GGCGCACTGC
    GCGCTGCTGC TCAACCTGAA GCTCTCCCAC GGTGACTACT ACCGCAAGCG GCCCACCCTA
    GCACCCAGCA CCATGAAAGA GTTCATGAAG CAGGTCCAGA CCAGCGGGGT GCTGATGGTC
    TTCTCCCAGG CCTGGATTGA GGAACTTTAC CACCAGGTTC TTGACAGAAA CATGCTCGGA
    GAAGCCGGCT ACTGGGGCAG CCCTGAAGAC AACAGCCTCC CCCTCATCAC CATGCTGACG
    GATATCGATG GCTTAGAGAG CAGTGCAATT GGAGGGCAGC TGATGGCCTC AGCTTCGATG
    GAGTCTCCTT TCACCCAGGG CAGGAGAATT GATGACTCCA CGGTGGCAGG TGTGGCATTT
    GCTCGCTATA TTCTGATTGG CTGCTGGAAG AACTTGATCG ACACTTTATC AACCCCCCTG
    ACGGGCCGAA TGGCCGGCAG CTCCAAAGGG CTGGCCTTCA TCTTGGGAGC TGAAGGCATC
    AAAGAACAGA ACCAAAAAGA ACGCGATGCT ATCTGCATGA GCCTCGACGG GCTACGGAAA
    GCCGCGCGTC TGAGCTGCGC TCTAGGAGTT GCAGCCAACT GTGCTTCGGC CCTCGCCCAG
    ATGGCAGCTG CCTCCTGTGT CCAGGAGGAG AAAGAGGAAA AGGAAACCCA GGAACCCAGT
    GATGCCATAG CACAAGTGAA ACTAAAAGTG GAGCAGAAGC TGGAGCAGAT CGGGAAGCTG
    CAGGGGGTGT GGCTGCACAC AGCCCACGTC CTGTGCATGG ACGCCATCCT CAGTGTCGGC
    CTGGAGATGG GCAGCCACAA CCCCGACTGC TGGCCACACG TGTTCAGGGT GTGCGAGTAC
    GTGGGCACCT TGGAACACAC CCACTTCAGC GATGGCACCT CTCAGCCCCC GCTGACCATC
    AGCCAGCCCC AGAAGGCCCC CAGAGGCGGA CTCCTTGGGG ACCTGGAGAG TGAGGGCTCA
    GCCCAGGAGC AGAGCCTGGA GCAGGAGAGC TCCCTGAGCG CGGCCCCCGT CATCCAACCG
    CTCTCCATCC AGGAGCTCGT CCGAGAAGGC AGCCGAGGCC GGGCCTCTGA CTTCCGTGGG
    GGCAGCCTCA TGACAGGCAG CAGTGCCGCC AAGGTGGTGC TCACGCTCTC CACGCAGGCT
    GACAGGCTCT TTGAAGATGC TACAGATAAA CTGAACCTCA TGGCTCTGGG AGGTTTTCTT
    TACCAGCTGA AGAAAGCATC GCAGTCCCAG CTTTTCCATT CTGTTACGGA AACAGTTGAT
    TACTCGCTGG CAATGCCAGG AGAAGTGAAG TCTACCCAAG ACCGCAAAAG TGCCCTCCAC
    CTGTTCCGCC TCGGGGACGC CATGCTAAGG ATCGTGAGGA GCAAATCGAG GCCCCTGCTC
    CACGTGATGC GCTGCTGGAG CCTCGTGGCC CCACACCTGG TAGAGGCTGC CTGCCATAAG
    GAAAGACACG TGTCTCAGAA GGCGGTTTCC TTCATCCATG ACATACTGAC GGAGGTTCTC
    ACGGACTGGA ATGAGCCACC TCATTTCCAC TTTAACGAAG CTCTCTTCCG ACCGTTCGAA
    CGTATCATGC AGCTGGAGTT GTGTGACGAG GACGTCCAAG ACCAGGTTGT CACGTCCATC
    GGGGAGCTGG TTGAAGTGTG CTCTGCCCAG ATCCAGTCAG GCTGGAGGCC CCTGTTCAGC
    GCCCTGGAAA CGGTGCACAG CGGGAACAAG TCCGAGGTGA AGGAGTACCT GGTGGGCGAC
    TATTCCATGG GAAAAGGCCA GGCTCCAGTG TTTGATGTAT TTGAAGCTTT TCTCAATACC
    GACAACATCC AGGTCTTTGC TAATGCAGCC ACTAGTTACA TCATGTGCCT TATGAAGTTT
    GTCAAAGGAC TGGGGGAGGT GGACTGTAAG GAGATTGGAG ACTGTGTCCC AGGACCCGGA
    GCCGCGTCCC CAGACCTGTG CCTCCCCGCC CTGGACTACC TCCGGCGCTG TTCTCAGTTG
    TTGGCCAAAA TCTACAAAAT GCCCTTGAAG CCAATATTCC TTAGTGGGCG ACTTGCTAGC
    TTGCCCCGAA GACTTCAGGA GCAGTCAGCG AGCAGTGAGG ATGGAATTGA GTCAGTCTTG
    TCTGACTTTG ATGACGACAC TGGTCTGATC GAAGTTTGGA TCATCCTCTT GGAGCAGCTC
    ACAGCAGCTG TGTCCAACTG TCCACGTCAG CACCAACCCC CCACTCTGGA TTTGCTCTTT
    GAGCTGTTGA GAGAGGTTAC CAAAACACCT GGACCAGGGT TTGGCATCTA TGCAGTTGTT
    CACCTTCTCC TTCCTGTGAT GTCTCTTTGG CTTCGCCGTA GCCATAAAGA CCATTCTTAC
    TGGGACATTG CCTCGGCTAA CTTCAAGCAT GCCATTGGTC TGTCCTGTGA GCTGGTGGTG
    GAGCACATTC AGAGCTTTAT CCACTCAGAC ATCAGGTATG AGAGCATGAT CAACACCATG
    CTGAAGGACC TCTTTGAGCT ACTGGTAGCA TGTGTAGCCA AGCCCACAGA AACCATCTCC
    CGAGTAGGCT GCTCCTGTAT TAGGTACGTC CTCGTGACAG CGGGCCCCGT GTTCACGGAG
    GAGATGTGGA GGCTGGCCTG CTGTGCCCTG CAGGACGCCT TCTCGGCCAC ACTCAAGCCG
    GTGAAGGACC TGCTGGGCTG CTTCCACAGT GGCACCGAGA GCTTCAGCGG CGAAGGCTGC
    CAGGTGAGGG TGGCCGCCCC GTCCTCCTCC CCGAGTGCCG AGGCCGAGTA CTGGCGCATC
    CGAGCCATGG CTCAGCAGGT ATTTATGTTG GATACCCAGT GCTCACCGAA GACTCCAAAC
    AACTTCGATC ATGCTCAGTC CTGTCAGCTC ATTATTGAAC TGCCTCCTGA TGAGAAGCCA
    AATGGGCATA CCAAGAAAAG TGTTTCTTTC AGGGAAATTG TGGTGAGTTT ACTATCCCAT
    CAAGTATTAC TCCAGAACTT ATATGATATC TTATTAGAAG AGTTTGTCAA AGGCCCCTCT
    CCTGGGGAGG AGAAGATCCA AGGGCCAGAA ACCAAGCTGG CTGGCTTCCT CAGATACATC
    TCCATGCAGA ACCTGGCCGT CATCTTTGAC CTTCTGCTGG ACTCCTACAG GACGGCCAGA
    GAGTTTGACA CCAGCCCAGG GCTGAAGTGC CTGCTGAAGA AAGTGTCTGG CATAGGGGGC
    GCTGCCAACC TCTACCGCCA GTCTGCCATG AGTTTCAACA TTTATTTCCA CGCACTGGTC
    TGTGCTGTTC TTAGCAACCA AGAAACCATC ACTGCTGACC AAGTGAAGAA GGTCCTTTTT
    GAGGACGATG AGAGGAGCAC AGATTCGTCC CAGCAGTGTT CATCAGAAGA TGAAGACATT
    TTTGAGGAGA CTGCCCAGGT GAGCCCCCCG AGAGGCAAAG AAAAGAGGCA GTGGAGGGCT
    CGGATGCCCT CGCTCAGCGT CCAGCCCGTC AGCAACGCGG ACTGGGTGTG GCTGGTCAAG
    AGGCTTCACA AGCTGTGCAT GGAGCTGTGT AACAACTACA TCCAGATGCA CCTGGACCTG
    GAAAACAGTA TGGAGGAGGC CCCAATCTTC AAGAGTGACC CTTTCTTCAT CCTTCCCTCC
    TTCCAGTCGG AGTCCTCCAC CCCTTCCACC GGGGGCTTCT CAGGGAAAGA CACGCCTTCC
    GAGGATGACC GCAGTCAGAC CCGGGATCAT GCGGGCGAGC CTCTGAGCCT GAGGGGGACC
    AGCGGGGAGC TGCTGCTGCT GCCCCCGAGC CCCAAGGTGG AGAAGAAGGA TCCCAGCCGC
    AAGAAGGAGT GGTGGGAGAA CGCGGGCAAC AAAATCTATA CCATGGCAGC CGACAAGACC
    ATCTCAAAGC TGATGACCGA GTACAAGAGG AGGAAACAGC AGCACAACCT GCCTGTCTTC
    CCCAAAGAGA TCAAGGTGGA GAAGAAGGGA GAGGTGCTGG GCGCCAGGGG CCAAGGCTCC
    CCACTGCTCC AGCGACCCCA GCACCTGATG GACCAGGGGC AGATGCGGCA CTCTTTCAGC
    GCCGGCCCTG AGCTGCTGCG CCAGGAGAAG AGGCCCCGCT CCGGCTCCAC CGGCAGCTCC
    CGGAGTGTCT CCGCGAGGGA CGCAGAAGCC CAGATCCAGG CATGGACCAA CATGGTGCTA
    ACAGTCCTCA ATCAGATTCA GATCCTCCCA GACCAGACCT TCACAGCCCT CCAGCCAGCA
    GTGTTTCCGT GTATCAGCCA GCTCACCTGT CACGTGACTG ACATCAGAGT TCGCCAGGCT
    GTGAGAGAAT GGCTCGGCAG AGTGGGCCGT GTCTATGACA TCATTATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690320.1
    CDS181..6711
    Translation

    Target ORF information:

    RefSeq Version XM_002690274.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690274.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    GTGTGCATTG AAACATACAT ATGTAGCTGT CACCAGCGTA GTATAAACAC TGCTGTGCGG
    GCAACTCTCA GTCAAATGCT GAGTGACTTG ACTTTACAGT TACGACAAAG GCAGGAGAAC
    ACGATAATTG AAAACCCGGA TGTCCCCCAG GAATTCAGGA GTCAAGGGTC AACAGTAGAG
    TCCCTCTGTG ATGACCTTGT CTCCGTGCTC ACTGTTCTGT GTGAGAAGCT CCAAGCTGCC
    ATCAATGACA GCCAGCAGCT GCAGCTCCTG TACCTGGAGT GCATCCTGTC CGTGCTCAGC
    AGCTCCTCCT CCTCCATGCA GCTGCACCGA GGCTTCACAG ACCTCATCTG GAAGAACCTT
    TGCCCCGCTC TCATCGTGAT CCTGGGGAAT CCGATTCATG ATAAAACCAT CACCTCTGCA
    CACAGCAGCA GCACGAGTAC CAGCATCGAG TCAGACTCTG TGTCTCCTGG GGTTTCCGAC
    CACGGCCGGG GTTCGGGCTG CTCCTCCACA GCGCCAGCCC TGAGTGGGCC AGTGGCCCGG
    ACCATCTACT ACATCGCGGC TGAGCTGGTG CGGCTGGTGG GGTCGGTGGA CTCCATGAAG
    CCCGTGTTGC AGTCCCTCTA CCACCGTGTG CTGCTCTACC CCCCACCCCA GCACCGTGTG
    GAAGCCATCA AGATCATGAA AGAGCTACTT GGGAGCCCAA AGCGTCTCTG TGATTTGGCA
    GGACCCAGCT CCACTGAACC TGACTCGAGA AAAAGATCAA TTTCAAAAAG AAAGTCTCAT
    CTGGATCTTC TCAAACTCAT CATGGACGGC ATGACCGAGG CGTGTATCAA GGGCGGCATC
    GAGGCCTGCT ACGCCGCCGT GTCCTGCGTC TGCACCTTGC TGGGAGCCTT GGAGGAGCTC
    AGCCAGGGCA GGGGCTTGAG CGAAAGTCAA GTCCAGCTGC TGCTGCTGCG CCTGGAGGAG
    CTGAAGGACG GGGCCGAGTC CAGCCGCGAC TCCATGGAGA TCAATGAGGC CGACTTCCGC
    TGGCAGCGGC GAGTCCTGTC CTCGGAACAC ACACCCTGGG AGTTGGGGAA TGAGCGGAGC
    CTTGACATCA GCATCAGCGT TACCACGGAC ACGGGCCAGA CCACCTTGGA GGGAGAGTTG
    GGTCAGACCA CACCCGAGGA CCACTCGGAA AACCCCAAGA ATGGCCTCAA GTCACCAGCC
    GTCCAGGAGG GCAAGGAGAC TCTGAGTAAA GTGTCAGAAC CAGAAGCTGT GGACCAGCCA
    GACGTGGTTC AAAGGAGCCA CACGGTTGCC TACCCCGATA TAACCAACTT CCTGTCCGTC
    GACTGCAGGA TGAGGTCCTA CGGATCCAGA TACAGTGAGA GCAACTTCAG CGTGGACGAC
    CAAGACCTCT CTCGGACAGA ATTCGACTCC TGTGACCAGT ACTCCATGGC GGCTGAAAAG
    GACTCCGGGA GATCGGACGT GTCGGACATT GGATCCGACA ACTGTTCTTT AGCTGATGAG
    GAGCAGACCC CCCGGGACTG CCTGGGCCAC AGGTCCCTGC GGACTGCAGC TCTGTCTCTC
    AAGCTGCTGA AGAACCAGGA GGCTGACCAG CACAGCGCCC GGCTGTTCGT GCAGTCGCTG
    GAGGGTCTCC TGCCGCGCCT CCTGGCCCTG CCCAGTGTGG AAGAGGTGGA CTCAGCCCTC
    CAGAACTTTG TCTCTACCTT CTGCTCAGGT ATGATGCATT CACCAGGGTT TGATGGGAAC
    AGCAGCCTGG GCCTGCAGAT GCTGATGAAC GCGGACAGCC TGTACTCGGC GGCGCACTGC
    GCGCTGCTGC TCAACCTGAA GCTCTCCCAC GGTGACTACT ACCGCAAGCG GCCCACCCTA
    GCACCCAGCA CCATGAAAGA GTTCATGAAG CAGGTCCAGA CCAGCGGGGT GCTGATGGTC
    TTCTCCCAGG CCTGGATTGA GGAACTTTAC CACCAGGTTC TTGACAGAAA CATGCTCGGA
    GAAGCCGGCT ACTGGGGCAG CCCTGAAGAC AACAGCCTCC CCCTCATCAC CATGCTGACG
    GATATCGATG GCTTAGAGAG CAGTGCAATT GGAGGGCAGC TGATGGCCTC AGCTTCGATG
    GAGTCTCCTT TCACCCAGGG CAGGAGAATT GATGACTCCA CGGTGGCAGG TGTGGCATTT
    GCTCGCTATA TTCTGATTGG CTGCTGGAAG AACTTGATCG ACACTTTATC AACCCCCCTG
    ACGGGCCGAA TGGCCGGCAG CTCCAAAGGG CTGGCCTTCA TCTTGGGAGC TGAAGGCATC
    AAAGAACAGA ACCAAAAAGA ACGCGATGCT ATCTGCATGA GCCTCGACGG GCTACGGAAA
    GCCGCGCGTC TGAGCTGCGC TCTAGGAGTT GCAGCCAACT GTGCTTCGGC CCTCGCCCAG
    ATGGCAGCTG CCTCCTGTGT CCAGGAGGAG AAAGAGGAAA AGGAAACCCA GGAACCCAGT
    GATGCCATAG CACAAGTGAA ACTAAAAGTG GAGCAGAAGC TGGAGCAGAT CGGGAAGCTG
    CAGGGGGTGT GGCTGCACAC AGCCCACGTC CTGTGCATGG ACGCCATCCT CAGTGTCGGC
    CTGGAGATGG GCAGCCACAA CCCCGACTGC TGGCCACACG TGTTCAGGGT GTGCGAGTAC
    GTGGGCACCT TGGAACACAC CCACTTCAGC GATGGCACCT CTCAGCCCCC GCTGACCATC
    AGCCAGCCCC AGAAGGCCCC CAGAGGCGGA CTCCTTGGGG ACCTGGAGAG TGAGGGCTCA
    GCCCAGGAGC AGAGCCTGGA GCAGGAGAGC TCCCTGAGCG CGGCCCCCGT CATCCAACCG
    CTCTCCATCC AGGAGCTCGT CCGAGAAGGC AGCCGAGGCC GGGCCTCTGA CTTCCGTGGG
    GGCAGCCTCA TGACAGGCAG CAGTGCCGCC AAGGTGGTGC TCACGCTCTC CACGCAGGCT
    GACAGGCTCT TTGAAGATGC TACAGATAAA CTGAACCTCA TGGCTCTGGG AGGTTTTCTT
    TACCAGCTGA AGAAAGCATC GCAGTCCCAG CTTTTCCATT CTGTTACGGA AACAGTTGAT
    TACTCGCTGG CAATGCCAGG AGAAGTGAAG TCTACCCAAG ACCGCAAAAG TGCCCTCCAC
    CTGTTCCGCC TCGGGGACGC CATGCTAAGG ATCGTGAGGA GCAAATCGAG GCCCCTGCTC
    CACGTGATGC GCTGCTGGAG CCTCGTGGCC CCACACCTGG TAGAGGCTGC CTGCCATAAG
    GAAAGACACG TGTCTCAGAA GGCGGTTTCC TTCATCCATG ACATACTGAC GGAGGTTCTC
    ACGGACTGGA ATGAGCCACC TCATTTCCAC TTTAACGAAG CTCTCTTCCG ACCGTTCGAA
    CGTATCATGC AGCTGGAGTT GTGTGACGAG GACGTCCAAG ACCAGGTTGT CACGTCCATC
    GGGGAGCTGG TTGAAGTGTG CTCTGCCCAG ATCCAGTCAG GCTGGAGGCC CCTGTTCAGC
    GCCCTGGAAA CGGTGCACAG CGGGAACAAG TCCGAGGTGA AGGAGTACCT GGTGGGCGAC
    TATTCCATGG GAAAAGGCCA GGCTCCAGTG TTTGATGTAT TTGAAGCTTT TCTCAATACC
    GACAACATCC AGGTCTTTGC TAATGCAGCC ACTAGTTACA TCATGTGCCT TATGAAGTTT
    GTCAAAGGAC TGGGGGAGGT GGACTGTAAG GAGATTGGAG ACTGTGTCCC AGGACCCGGA
    GCCGCGTCCC CAGACCTGTG CCTCCCCGCC CTGGACTACC TCCGGCGCTG TTCTCAGTTG
    TTGGCCAAAA TCTACAAAAT GCCCTTGAAG CCAATATTCC TTAGTGGGCG ACTTGCTAGC
    TTGCCCCGAA GACTTCAGGA GCAGTCAGCG AGCAGTGAGG ATGGAATTGA GTCAGTCTTG
    TCTGACTTTG ATGACGACAC TGGTCTGATC GAAGTTTGGA TCATCCTCTT GGAGCAGCTC
    ACAGCAGCTG TGTCCAACTG TCCACGTCAG CACCAACCCC CCACTCTGGA TTTGCTCTTT
    GAGCTGTTGA GAGAGGTTAC CAAAACACCT GGACCAGGGT TTGGCATCTA TGCAGTTGTT
    CACCTTCTCC TTCCTGTGAT GTCTCTTTGG CTTCGCCGTA GCCATAAAGA CCATTCTTAC
    TGGGACATTG CCTCGGCTAA CTTCAAGCAT GCCATTGGTC TGTCCTGTGA GCTGGTGGTG
    GAGCACATTC AGAGCTTTAT CCACTCAGAC ATCAGGTATG AGAGCATGAT CAACACCATG
    CTGAAGGACC TCTTTGAGCT ACTGGTAGCA TGTGTAGCCA AGCCCACAGA AACCATCTCC
    CGAGTAGGCT GCTCCTGTAT TAGGTACGTC CTCGTGACAG CGGGCCCCGT GTTCACGGAG
    GAGATGTGGA GGCTGGCCTG CTGTGCCCTG CAGGACGCCT TCTCGGCCAC ACTCAAGCCG
    GTGAAGGACC TGCTGGGCTG CTTCCACAGT GGCACCGAGA GCTTCAGCGG CGAAGGCTGC
    CAGGTGAGGG TGGCCGCCCC GTCCTCCTCC CCGAGTGCCG AGGCCGAGTA CTGGCGCATC
    CGAGCCATGG CTCAGCAGGT ATTTATGTTG GATACCCAGT GCTCACCGAA GACTCCAAAC
    AACTTCGATC ATGCTCAGTC CTGTCAGCTC ATTATTGAAC TGCCTCCTGA TGAGAAGCCA
    AATGGGCATA CCAAGAAAAG TGTTTCTTTC AGGGAAATTG TGGTGAGTTT ACTATCCCAT
    CAAGTATTAC TCCAGAACTT ATATGATATC TTATTAGAAG AGTTTGTCAA AGGCCCCTCT
    CCTGGGGAGG AGAAGATCCA AGGGCCAGAA ACCAAGCTGG CTGGCTTCCT CAGATACATC
    TCCATGCAGA ACCTGGCCGT CATCTTTGAC CTTCTGCTGG ACTCCTACAG GACGGCCAGA
    GAGTTTGACA CCAGCCCAGG GCTGAAGTGC CTGCTGAAGA AAGTGTCTGG CATAGGGGGC
    GCTGCCAACC TCTACCGCCA GTCTGCCATG AGTTTCAACA TTTATTTCCA CGCACTGGTC
    TGTGCTGTTC TTAGCAACCA AGAAACCATC ACTGCTGACC AAGTGAAGAA GGTCCTTTTT
    GAGGACGATG AGAGGAGCAC AGATTCGTCC CAGCAGTGTT CATCAGAAGA TGAAGACATT
    TTTGAGGAGA CTGCCCAGGT GAGCCCCCCG AGAGGCAAAG AAAAGAGGCA GTGGAGGGCT
    CGGATGCCCT CGCTCAGCGT CCAGCCCGTC AGCAACGCGG ACTGGGTGTG GCTGGTCAAG
    AGGCTTCACA AGCTGTGCAT GGAGCTGTGT AACAACTACA TCCAGATGCA CCTGGACCTG
    GAAAACAGTA TGGAGGAGGC CCCAATCTTC AAGAGTGACC CTTTCTTCAT CCTTCCCTCC
    TTCCAGTCGG AGTCCTCCAC CCCTTCCACC GGGGGCTTCT CAGGGAAAGA CACGCCTTCC
    GAGGATGACC GCAGTCAGAC CCGGGATCAT GCGGGCGAGC CTCTGAGCCT GAGGGGGACC
    AGCGGGGAGC TGCTGCTGCT GCCCCCGAGC CCCAAGGTGG AGAAGAAGGA TCCCAGCCGC
    AAGAAGGAGT GGTGGGAGAA CGCGGGCAAC AAAATCTATA CCATGGCAGC CGACAAGACC
    ATCTCAAAGC TGATGACCGA GTACAAGAGG AGGAAACAGC AGCACAACCT GCCTGTCTTC
    CCCAAAGAGA TCAAGGTGGA GAAGAAGGGA GAGGTGCTGG GCGCCAGGGG CCAAGGCTCC
    CCACTGCTCC AGCGACCCCA GCACCTGATG GACCAGGGGC AGATGCGGCA CTCTTTCAGC
    GCCGGCCCTG AGCTGCTGCG CCAGGAGAAG AGGCCCCGCT CCGGCTCCAC CGGCAGCTCC
    CGGAGTGTCT CCGCGAGGGA CGCAGAAGCC CAGATCCAGG CATGGACCAA CATGGTGCTA
    ACAGTCCTCA ATCAGATTCA GATCCTCCCA GACCAGACCT TCACAGCCCT CCAGCCAGCA
    GTGTTTCCGT GTATCAGCCA GCTCACCTGT CACGTGACTG ACATCAGAGT TCGCCAGGCT
    GTGAGAGAAT GGCTCGGCAG AGTGGGCCGT GTCTATGACA TCATTATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22720
    Clone ID Related Accession (Same CDS sequence) XM_015464837.1 , XM_002690274.4 , XM_002690274.5
    Accession Version XM_002690274.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690274.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    GTGTGCATTG AAACATACAT ATGTAGCTGT CACCAGCGTA GTATAAACAC TGCTGTGCGG
    GCAACTCTCA GTCAAATGCT GAGTGACTTG ACTTTACAGT TACGACAAAG GCAGGAGAAC
    ACGATAATTG AAAACCCGGA TGTCCCCCAG GAATTCAGGA GTCAAGGGTC AACAGTAGAG
    TCCCTCTGTG ATGACCTTGT CTCCGTGCTC ACTGTTCTGT GTGAGAAGCT CCAAGCTGCC
    ATCAATGACA GCCAGCAGCT GCAGCTCCTG TACCTGGAGT GCATCCTGTC CGTGCTCAGC
    AGCTCCTCCT CCTCCATGCA GCTGCACCGA GGCTTCACAG ACCTCATCTG GAAGAACCTT
    TGCCCCGCTC TCATCGTGAT CCTGGGGAAT CCGATTCATG ATAAAACCAT CACCTCTGCA
    CACAGCAGCA GCACGAGTAC CAGCATCGAG TCAGACTCTG TGTCTCCTGG GGTTTCCGAC
    CACGGCCGGG GTTCGGGCTG CTCCTCCACA GCGCCAGCCC TGAGTGGGCC AGTGGCCCGG
    ACCATCTACT ACATCGCGGC TGAGCTGGTG CGGCTGGTGG GGTCGGTGGA CTCCATGAAG
    CCCGTGTTGC AGTCCCTCTA CCACCGTGTG CTGCTCTACC CCCCACCCCA GCACCGTGTG
    GAAGCCATCA AGATCATGAA AGAGCTACTT GGGAGCCCAA AGCGTCTCTG TGATTTGGCA
    GGACCCAGCT CCACTGAACC TGACTCGAGA AAAAGATCAA TTTCAAAAAG AAAGTCTCAT
    CTGGATCTTC TCAAACTCAT CATGGACGGC ATGACCGAGG CGTGTATCAA GGGCGGCATC
    GAGGCCTGCT ACGCCGCCGT GTCCTGCGTC TGCACCTTGC TGGGAGCCTT GGAGGAGCTC
    AGCCAGGGCA GGGGCTTGAG CGAAAGTCAA GTCCAGCTGC TGCTGCTGCG CCTGGAGGAG
    CTGAAGGACG GGGCCGAGTC CAGCCGCGAC TCCATGGAGA TCAATGAGGC CGACTTCCGC
    TGGCAGCGGC GAGTCCTGTC CTCGGAACAC ACACCCTGGG AGTTGGGGAA TGAGCGGAGC
    CTTGACATCA GCATCAGCGT TACCACGGAC ACGGGCCAGA CCACCTTGGA GGGAGAGTTG
    GGTCAGACCA CACCCGAGGA CCACTCGGAA AACCCCAAGA ATGGCCTCAA GTCACCAGCC
    GTCCAGGAGG GCAAGGAGAC TCTGAGTAAA GTGTCAGAAC CAGAAGCTGT GGACCAGCCA
    GACGTGGTTC AAAGGAGCCA CACGGTTGCC TACCCCGATA TAACCAACTT CCTGTCCGTC
    GACTGCAGGA TGAGGTCCTA CGGATCCAGA TACAGTGAGA GCAACTTCAG CGTGGACGAC
    CAAGACCTCT CTCGGACAGA ATTCGACTCC TGTGACCAGT ACTCCATGGC GGCTGAAAAG
    GACTCCGGGA GATCGGACGT GTCGGACATT GGATCCGACA ACTGTTCTTT AGCTGATGAG
    GAGCAGACCC CCCGGGACTG CCTGGGCCAC AGGTCCCTGC GGACTGCAGC TCTGTCTCTC
    AAGCTGCTGA AGAACCAGGA GGCTGACCAG CACAGCGCCC GGCTGTTCGT GCAGTCGCTG
    GAGGGTCTCC TGCCGCGCCT CCTGGCCCTG CCCAGTGTGG AAGAGGTGGA CTCAGCCCTC
    CAGAACTTTG TCTCTACCTT CTGCTCAGGT ATGATGCATT CACCAGGGTT TGATGGGAAC
    AGCAGCCTGG GCCTGCAGAT GCTGATGAAC GCGGACAGCC TGTACTCGGC GGCGCACTGC
    GCGCTGCTGC TCAACCTGAA GCTCTCCCAC GGTGACTACT ACCGCAAGCG GCCCACCCTA
    GCACCCAGCA CCATGAAAGA GTTCATGAAG CAGGTCCAGA CCAGCGGGGT GCTGATGGTC
    TTCTCCCAGG CCTGGATTGA GGAACTTTAC CACCAGGTTC TTGACAGAAA CATGCTCGGA
    GAAGCCGGCT ACTGGGGCAG CCCTGAAGAC AACAGCCTCC CCCTCATCAC CATGCTGACG
    GATATCGATG GCTTAGAGAG CAGTGCAATT GGAGGGCAGC TGATGGCCTC AGCTTCGATG
    GAGTCTCCTT TCACCCAGGG CAGGAGAATT GATGACTCCA CGGTGGCAGG TGTGGCATTT
    GCTCGCTATA TTCTGATTGG CTGCTGGAAG AACTTGATCG ACACTTTATC AACCCCCCTG
    ACGGGCCGAA TGGCCGGCAG CTCCAAAGGG CTGGCCTTCA TCTTGGGAGC TGAAGGCATC
    AAAGAACAGA ACCAAAAAGA ACGCGATGCT ATCTGCATGA GCCTCGACGG GCTACGGAAA
    GCCGCGCGTC TGAGCTGCGC TCTAGGAGTT GCAGCCAACT GTGCTTCGGC CCTCGCCCAG
    ATGGCAGCTG CCTCCTGTGT CCAGGAGGAG AAAGAGGAAA AGGAAACCCA GGAACCCAGT
    GATGCCATAG CACAAGTGAA ACTAAAAGTG GAGCAGAAGC TGGAGCAGAT CGGGAAGCTG
    CAGGGGGTGT GGCTGCACAC AGCCCACGTC CTGTGCATGG ACGCCATCCT CAGTGTCGGC
    CTGGAGATGG GCAGCCACAA CCCCGACTGC TGGCCACACG TGTTCAGGGT GTGCGAGTAC
    GTGGGCACCT TGGAACACAC CCACTTCAGC GATGGCACCT CTCAGCCCCC GCTGACCATC
    AGCCAGCCCC AGAAGGCCCC CAGAGGCGGA CTCCTTGGGG ACCTGGAGAG TGAGGGCTCA
    GCCCAGGAGC AGAGCCTGGA GCAGGAGAGC TCCCTGAGCG CGGCCCCCGT CATCCAACCG
    CTCTCCATCC AGGAGCTCGT CCGAGAAGGC AGCCGAGGCC GGGCCTCTGA CTTCCGTGGG
    GGCAGCCTCA TGACAGGCAG CAGTGCCGCC AAGGTGGTGC TCACGCTCTC CACGCAGGCT
    GACAGGCTCT TTGAAGATGC TACAGATAAA CTGAACCTCA TGGCTCTGGG AGGTTTTCTT
    TACCAGCTGA AGAAAGCATC GCAGTCCCAG CTTTTCCATT CTGTTACGGA AACAGTTGAT
    TACTCGCTGG CAATGCCAGG AGAAGTGAAG TCTACCCAAG ACCGCAAAAG TGCCCTCCAC
    CTGTTCCGCC TCGGGGACGC CATGCTAAGG ATCGTGAGGA GCAAATCGAG GCCCCTGCTC
    CACGTGATGC GCTGCTGGAG CCTCGTGGCC CCACACCTGG TAGAGGCTGC CTGCCATAAG
    GAAAGACACG TGTCTCAGAA GGCGGTTTCC TTCATCCATG ACATACTGAC GGAGGTTCTC
    ACGGACTGGA ATGAGCCACC TCATTTCCAC TTTAACGAAG CTCTCTTCCG ACCGTTCGAA
    CGTATCATGC AGCTGGAGTT GTGTGACGAG GACGTCCAAG ACCAGGTTGT CACGTCCATC
    GGGGAGCTGG TTGAAGTGTG CTCTGCCCAG ATCCAGTCAG GCTGGAGGCC CCTGTTCAGC
    GCCCTGGAAA CGGTGCACAG CGGGAACAAG TCCGAGGTGA AGGAGTACCT GGTGGGCGAC
    TATTCCATGG GAAAAGGCCA GGCTCCAGTG TTTGATGTAT TTGAAGCTTT TCTCAATACC
    GACAACATCC AGGTCTTTGC TAATGCAGCC ACTAGTTACA TCATGTGCCT TATGAAGTTT
    GTCAAAGGAC TGGGGGAGGT GGACTGTAAG GAGATTGGAG ACTGTGTCCC AGGACCCGGA
    GCCGCGTCCC CAGACCTGTG CCTCCCCGCC CTGGACTACC TCCGGCGCTG TTCTCAGTTG
    TTGGCCAAAA TCTACAAAAT GCCCTTGAAG CCAATATTCC TTAGTGGGCG ACTTGCTAGC
    TTGCCCCGAA GACTTCAGGA GCAGTCAGCG AGCAGTGAGG ATGGAATTGA GTCAGTCTTG
    TCTGACTTTG ATGACGACAC TGGTCTGATC GAAGTTTGGA TCATCCTCTT GGAGCAGCTC
    ACAGCAGCTG TGTCCAACTG TCCACGTCAG CACCAACCCC CCACTCTGGA TTTGCTCTTT
    GAGCTGTTGA GAGAGGTTAC CAAAACACCT GGACCAGGGT TTGGCATCTA TGCAGTTGTT
    CACCTTCTCC TTCCTGTGAT GTCTCTTTGG CTTCGCCGTA GCCATAAAGA CCATTCTTAC
    TGGGACATTG CCTCGGCTAA CTTCAAGCAT GCCATTGGTC TGTCCTGTGA GCTGGTGGTG
    GAGCACATTC AGAGCTTTAT CCACTCAGAC ATCAGGTATG AGAGCATGAT CAACACCATG
    CTGAAGGACC TCTTTGAGCT ACTGGTAGCA TGTGTAGCCA AGCCCACAGA AACCATCTCC
    CGAGTAGGCT GCTCCTGTAT TAGGTACGTC CTCGTGACAG CGGGCCCCGT GTTCACGGAG
    GAGATGTGGA GGCTGGCCTG CTGTGCCCTG CAGGACGCCT TCTCGGCCAC ACTCAAGCCG
    GTGAAGGACC TGCTGGGCTG CTTCCACAGT GGCACCGAGA GCTTCAGCGG CGAAGGCTGC
    CAGGTGAGGG TGGCCGCCCC GTCCTCCTCC CCGAGTGCCG AGGCCGAGTA CTGGCGCATC
    CGAGCCATGG CTCAGCAGGT ATTTATGTTG GATACCCAGT GCTCACCGAA GACTCCAAAC
    AACTTCGATC ATGCTCAGTC CTGTCAGCTC ATTATTGAAC TGCCTCCTGA TGAGAAGCCA
    AATGGGCATA CCAAGAAAAG TGTTTCTTTC AGGGAAATTG TGGTGAGTTT ACTATCCCAT
    CAAGTATTAC TCCAGAACTT ATATGATATC TTATTAGAAG AGTTTGTCAA AGGCCCCTCT
    CCTGGGGAGG AGAAGATCCA AGGGCCAGAA ACCAAGCTGG CTGGCTTCCT CAGATACATC
    TCCATGCAGA ACCTGGCCGT CATCTTTGAC CTTCTGCTGG ACTCCTACAG GACGGCCAGA
    GAGTTTGACA CCAGCCCAGG GCTGAAGTGC CTGCTGAAGA AAGTGTCTGG CATAGGGGGC
    GCTGCCAACC TCTACCGCCA GTCTGCCATG AGTTTCAACA TTTATTTCCA CGCACTGGTC
    TGTGCTGTTC TTAGCAACCA AGAAACCATC ACTGCTGACC AAGTGAAGAA GGTCCTTTTT
    GAGGACGATG AGAGGAGCAC AGATTCGTCC CAGCAGTGTT CATCAGAAGA TGAAGACATT
    TTTGAGGAGA CTGCCCAGGT GAGCCCCCCG AGAGGCAAAG AAAAGAGGCA GTGGAGGGCT
    CGGATGCCCT CGCTCAGCGT CCAGCCCGTC AGCAACGCGG ACTGGGTGTG GCTGGTCAAG
    AGGCTTCACA AGCTGTGCAT GGAGCTGTGT AACAACTACA TCCAGATGCA CCTGGACCTG
    GAAAACAGTA TGGAGGAGGC CCCAATCTTC AAGAGTGACC CTTTCTTCAT CCTTCCCTCC
    TTCCAGTCGG AGTCCTCCAC CCCTTCCACC GGGGGCTTCT CAGGGAAAGA CACGCCTTCC
    GAGGATGACC GCAGTCAGAC CCGGGATCAT GCGGGCGAGC CTCTGAGCCT GAGGGGGACC
    AGCGGGGAGC TGCTGCTGCT GCCCCCGAGC CCCAAGGTGG AGAAGAAGGA TCCCAGCCGC
    AAGAAGGAGT GGTGGGAGAA CGCGGGCAAC AAAATCTATA CCATGGCAGC CGACAAGACC
    ATCTCAAAGC TGATGACCGA GTACAAGAGG AGGAAACAGC AGCACAACCT GCCTGTCTTC
    CCCAAAGAGA TCAAGGTGGA GAAGAAGGGA GAGGTGCTGG GCGCCAGGGG CCAAGGCTCC
    CCACTGCTCC AGCGACCCCA GCACCTGATG GACCAGGGGC AGATGCGGCA CTCTTTCAGC
    GCCGGCCCTG AGCTGCTGCG CCAGGAGAAG AGGCCCCGCT CCGGCTCCAC CGGCAGCTCC
    CGGAGTGTCT CCGCGAGGGA CGCAGAAGCC CAGATCCAGG CATGGACCAA CATGGTGCTA
    ACAGTCCTCA ATCAGATTCA GATCCTCCCA GACCAGACCT TCACAGCCCT CCAGCCAGCA
    GTGTTTCCGT GTATCAGCCA GCTCACCTGT CACGTGACTG ACATCAGAGT TCGCCAGGCT
    GTGAGAGAAT GGCTCGGCAG AGTGGGCCGT GTCTATGACA TCATTATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002690320.1
    CDS1..6531
    Translation

    Target ORF information:

    RefSeq Version XM_002690274.5
    Organism Bos taurus(cattle)
    Definition Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002690274.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    GTGTGCATTG AAACATACAT ATGTAGCTGT CACCAGCGTA GTATAAACAC TGCTGTGCGG
    GCAACTCTCA GTCAAATGCT GAGTGACTTG ACTTTACAGT TACGACAAAG GCAGGAGAAC
    ACGATAATTG AAAACCCGGA TGTCCCCCAG GAATTCAGGA GTCAAGGGTC AACAGTAGAG
    TCCCTCTGTG ATGACCTTGT CTCCGTGCTC ACTGTTCTGT GTGAGAAGCT CCAAGCTGCC
    ATCAATGACA GCCAGCAGCT GCAGCTCCTG TACCTGGAGT GCATCCTGTC CGTGCTCAGC
    AGCTCCTCCT CCTCCATGCA GCTGCACCGA GGCTTCACAG ACCTCATCTG GAAGAACCTT
    TGCCCCGCTC TCATCGTGAT CCTGGGGAAT CCGATTCATG ATAAAACCAT CACCTCTGCA
    CACAGCAGCA GCACGAGTAC CAGCATCGAG TCAGACTCTG TGTCTCCTGG GGTTTCCGAC
    CACGGCCGGG GTTCGGGCTG CTCCTCCACA GCGCCAGCCC TGAGTGGGCC AGTGGCCCGG
    ACCATCTACT ACATCGCGGC TGAGCTGGTG CGGCTGGTGG GGTCGGTGGA CTCCATGAAG
    CCCGTGTTGC AGTCCCTCTA CCACCGTGTG CTGCTCTACC CCCCACCCCA GCACCGTGTG
    GAAGCCATCA AGATCATGAA AGAGCTACTT GGGAGCCCAA AGCGTCTCTG TGATTTGGCA
    GGACCCAGCT CCACTGAACC TGACTCGAGA AAAAGATCAA TTTCAAAAAG AAAGTCTCAT
    CTGGATCTTC TCAAACTCAT CATGGACGGC ATGACCGAGG CGTGTATCAA GGGCGGCATC
    GAGGCCTGCT ACGCCGCCGT GTCCTGCGTC TGCACCTTGC TGGGAGCCTT GGAGGAGCTC
    AGCCAGGGCA GGGGCTTGAG CGAAAGTCAA GTCCAGCTGC TGCTGCTGCG CCTGGAGGAG
    CTGAAGGACG GGGCCGAGTC CAGCCGCGAC TCCATGGAGA TCAATGAGGC CGACTTCCGC
    TGGCAGCGGC GAGTCCTGTC CTCGGAACAC ACACCCTGGG AGTTGGGGAA TGAGCGGAGC
    CTTGACATCA GCATCAGCGT TACCACGGAC ACGGGCCAGA CCACCTTGGA GGGAGAGTTG
    GGTCAGACCA CACCCGAGGA CCACTCGGAA AACCCCAAGA ATGGCCTCAA GTCACCAGCC
    GTCCAGGAGG GCAAGGAGAC TCTGAGTAAA GTGTCAGAAC CAGAAGCTGT GGACCAGCCA
    GACGTGGTTC AAAGGAGCCA CACGGTTGCC TACCCCGATA TAACCAACTT CCTGTCCGTC
    GACTGCAGGA TGAGGTCCTA CGGATCCAGA TACAGTGAGA GCAACTTCAG CGTGGACGAC
    CAAGACCTCT CTCGGACAGA ATTCGACTCC TGTGACCAGT ACTCCATGGC GGCTGAAAAG
    GACTCCGGGA GATCGGACGT GTCGGACATT GGATCCGACA ACTGTTCTTT AGCTGATGAG
    GAGCAGACCC CCCGGGACTG CCTGGGCCAC AGGTCCCTGC GGACTGCAGC TCTGTCTCTC
    AAGCTGCTGA AGAACCAGGA GGCTGACCAG CACAGCGCCC GGCTGTTCGT GCAGTCGCTG
    GAGGGTCTCC TGCCGCGCCT CCTGGCCCTG CCCAGTGTGG AAGAGGTGGA CTCAGCCCTC
    CAGAACTTTG TCTCTACCTT CTGCTCAGGT ATGATGCATT CACCAGGGTT TGATGGGAAC
    AGCAGCCTGG GCCTGCAGAT GCTGATGAAC GCGGACAGCC TGTACTCGGC GGCGCACTGC
    GCGCTGCTGC TCAACCTGAA GCTCTCCCAC GGTGACTACT ACCGCAAGCG GCCCACCCTA
    GCACCCAGCA CCATGAAAGA GTTCATGAAG CAGGTCCAGA CCAGCGGGGT GCTGATGGTC
    TTCTCCCAGG CCTGGATTGA GGAACTTTAC CACCAGGTTC TTGACAGAAA CATGCTCGGA
    GAAGCCGGCT ACTGGGGCAG CCCTGAAGAC AACAGCCTCC CCCTCATCAC CATGCTGACG
    GATATCGATG GCTTAGAGAG CAGTGCAATT GGAGGGCAGC TGATGGCCTC AGCTTCGATG
    GAGTCTCCTT TCACCCAGGG CAGGAGAATT GATGACTCCA CGGTGGCAGG TGTGGCATTT
    GCTCGCTATA TTCTGATTGG CTGCTGGAAG AACTTGATCG ACACTTTATC AACCCCCCTG
    ACGGGCCGAA TGGCCGGCAG CTCCAAAGGG CTGGCCTTCA TCTTGGGAGC TGAAGGCATC
    AAAGAACAGA ACCAAAAAGA ACGCGATGCT ATCTGCATGA GCCTCGACGG GCTACGGAAA
    GCCGCGCGTC TGAGCTGCGC TCTAGGAGTT GCAGCCAACT GTGCTTCGGC CCTCGCCCAG
    ATGGCAGCTG CCTCCTGTGT CCAGGAGGAG AAAGAGGAAA AGGAAACCCA GGAACCCAGT
    GATGCCATAG CACAAGTGAA ACTAAAAGTG GAGCAGAAGC TGGAGCAGAT CGGGAAGCTG
    CAGGGGGTGT GGCTGCACAC AGCCCACGTC CTGTGCATGG ACGCCATCCT CAGTGTCGGC
    CTGGAGATGG GCAGCCACAA CCCCGACTGC TGGCCACACG TGTTCAGGGT GTGCGAGTAC
    GTGGGCACCT TGGAACACAC CCACTTCAGC GATGGCACCT CTCAGCCCCC GCTGACCATC
    AGCCAGCCCC AGAAGGCCCC CAGAGGCGGA CTCCTTGGGG ACCTGGAGAG TGAGGGCTCA
    GCCCAGGAGC AGAGCCTGGA GCAGGAGAGC TCCCTGAGCG CGGCCCCCGT CATCCAACCG
    CTCTCCATCC AGGAGCTCGT CCGAGAAGGC AGCCGAGGCC GGGCCTCTGA CTTCCGTGGG
    GGCAGCCTCA TGACAGGCAG CAGTGCCGCC AAGGTGGTGC TCACGCTCTC CACGCAGGCT
    GACAGGCTCT TTGAAGATGC TACAGATAAA CTGAACCTCA TGGCTCTGGG AGGTTTTCTT
    TACCAGCTGA AGAAAGCATC GCAGTCCCAG CTTTTCCATT CTGTTACGGA AACAGTTGAT
    TACTCGCTGG CAATGCCAGG AGAAGTGAAG TCTACCCAAG ACCGCAAAAG TGCCCTCCAC
    CTGTTCCGCC TCGGGGACGC CATGCTAAGG ATCGTGAGGA GCAAATCGAG GCCCCTGCTC
    CACGTGATGC GCTGCTGGAG CCTCGTGGCC CCACACCTGG TAGAGGCTGC CTGCCATAAG
    GAAAGACACG TGTCTCAGAA GGCGGTTTCC TTCATCCATG ACATACTGAC GGAGGTTCTC
    ACGGACTGGA ATGAGCCACC TCATTTCCAC TTTAACGAAG CTCTCTTCCG ACCGTTCGAA
    CGTATCATGC AGCTGGAGTT GTGTGACGAG GACGTCCAAG ACCAGGTTGT CACGTCCATC
    GGGGAGCTGG TTGAAGTGTG CTCTGCCCAG ATCCAGTCAG GCTGGAGGCC CCTGTTCAGC
    GCCCTGGAAA CGGTGCACAG CGGGAACAAG TCCGAGGTGA AGGAGTACCT GGTGGGCGAC
    TATTCCATGG GAAAAGGCCA GGCTCCAGTG TTTGATGTAT TTGAAGCTTT TCTCAATACC
    GACAACATCC AGGTCTTTGC TAATGCAGCC ACTAGTTACA TCATGTGCCT TATGAAGTTT
    GTCAAAGGAC TGGGGGAGGT GGACTGTAAG GAGATTGGAG ACTGTGTCCC AGGACCCGGA
    GCCGCGTCCC CAGACCTGTG CCTCCCCGCC CTGGACTACC TCCGGCGCTG TTCTCAGTTG
    TTGGCCAAAA TCTACAAAAT GCCCTTGAAG CCAATATTCC TTAGTGGGCG ACTTGCTAGC
    TTGCCCCGAA GACTTCAGGA GCAGTCAGCG AGCAGTGAGG ATGGAATTGA GTCAGTCTTG
    TCTGACTTTG ATGACGACAC TGGTCTGATC GAAGTTTGGA TCATCCTCTT GGAGCAGCTC
    ACAGCAGCTG TGTCCAACTG TCCACGTCAG CACCAACCCC CCACTCTGGA TTTGCTCTTT
    GAGCTGTTGA GAGAGGTTAC CAAAACACCT GGACCAGGGT TTGGCATCTA TGCAGTTGTT
    CACCTTCTCC TTCCTGTGAT GTCTCTTTGG CTTCGCCGTA GCCATAAAGA CCATTCTTAC
    TGGGACATTG CCTCGGCTAA CTTCAAGCAT GCCATTGGTC TGTCCTGTGA GCTGGTGGTG
    GAGCACATTC AGAGCTTTAT CCACTCAGAC ATCAGGTATG AGAGCATGAT CAACACCATG
    CTGAAGGACC TCTTTGAGCT ACTGGTAGCA TGTGTAGCCA AGCCCACAGA AACCATCTCC
    CGAGTAGGCT GCTCCTGTAT TAGGTACGTC CTCGTGACAG CGGGCCCCGT GTTCACGGAG
    GAGATGTGGA GGCTGGCCTG CTGTGCCCTG CAGGACGCCT TCTCGGCCAC ACTCAAGCCG
    GTGAAGGACC TGCTGGGCTG CTTCCACAGT GGCACCGAGA GCTTCAGCGG CGAAGGCTGC
    CAGGTGAGGG TGGCCGCCCC GTCCTCCTCC CCGAGTGCCG AGGCCGAGTA CTGGCGCATC
    CGAGCCATGG CTCAGCAGGT ATTTATGTTG GATACCCAGT GCTCACCGAA GACTCCAAAC
    AACTTCGATC ATGCTCAGTC CTGTCAGCTC ATTATTGAAC TGCCTCCTGA TGAGAAGCCA
    AATGGGCATA CCAAGAAAAG TGTTTCTTTC AGGGAAATTG TGGTGAGTTT ACTATCCCAT
    CAAGTATTAC TCCAGAACTT ATATGATATC TTATTAGAAG AGTTTGTCAA AGGCCCCTCT
    CCTGGGGAGG AGAAGATCCA AGGGCCAGAA ACCAAGCTGG CTGGCTTCCT CAGATACATC
    TCCATGCAGA ACCTGGCCGT CATCTTTGAC CTTCTGCTGG ACTCCTACAG GACGGCCAGA
    GAGTTTGACA CCAGCCCAGG GCTGAAGTGC CTGCTGAAGA AAGTGTCTGG CATAGGGGGC
    GCTGCCAACC TCTACCGCCA GTCTGCCATG AGTTTCAACA TTTATTTCCA CGCACTGGTC
    TGTGCTGTTC TTAGCAACCA AGAAACCATC ACTGCTGACC AAGTGAAGAA GGTCCTTTTT
    GAGGACGATG AGAGGAGCAC AGATTCGTCC CAGCAGTGTT CATCAGAAGA TGAAGACATT
    TTTGAGGAGA CTGCCCAGGT GAGCCCCCCG AGAGGCAAAG AAAAGAGGCA GTGGAGGGCT
    CGGATGCCCT CGCTCAGCGT CCAGCCCGTC AGCAACGCGG ACTGGGTGTG GCTGGTCAAG
    AGGCTTCACA AGCTGTGCAT GGAGCTGTGT AACAACTACA TCCAGATGCA CCTGGACCTG
    GAAAACAGTA TGGAGGAGGC CCCAATCTTC AAGAGTGACC CTTTCTTCAT CCTTCCCTCC
    TTCCAGTCGG AGTCCTCCAC CCCTTCCACC GGGGGCTTCT CAGGGAAAGA CACGCCTTCC
    GAGGATGACC GCAGTCAGAC CCGGGATCAT GCGGGCGAGC CTCTGAGCCT GAGGGGGACC
    AGCGGGGAGC TGCTGCTGCT GCCCCCGAGC CCCAAGGTGG AGAAGAAGGA TCCCAGCCGC
    AAGAAGGAGT GGTGGGAGAA CGCGGGCAAC AAAATCTATA CCATGGCAGC CGACAAGACC
    ATCTCAAAGC TGATGACCGA GTACAAGAGG AGGAAACAGC AGCACAACCT GCCTGTCTTC
    CCCAAAGAGA TCAAGGTGGA GAAGAAGGGA GAGGTGCTGG GCGCCAGGGG CCAAGGCTCC
    CCACTGCTCC AGCGACCCCA GCACCTGATG GACCAGGGGC AGATGCGGCA CTCTTTCAGC
    GCCGGCCCTG AGCTGCTGCG CCAGGAGAAG AGGCCCCGCT CCGGCTCCAC CGGCAGCTCC
    CGGAGTGTCT CCGCGAGGGA CGCAGAAGCC CAGATCCAGG CATGGACCAA CATGGTGCTA
    ACAGTCCTCA ATCAGATTCA GATCCTCCCA GACCAGACCT TCACAGCCCT CCAGCCAGCA
    GTGTTTCCGT GTATCAGCCA GCTCACCTGT CACGTGACTG ACATCAGAGT TCGCCAGGCT
    GTGAGAGAAT GGCTCGGCAG AGTGGGCCGT GTCTATGACA TCATTATGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22722
    Clone ID Related Accession (Same CDS sequence) XM_005210993.3 , XM_005210993.4 , XM_015464838.1
    Accession Version XM_005210993.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104223.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    ATAATTGAAA ACCCGGATGT CCCCCAGGAA TTCAGGAGTC AAGGGTCAAC AGTAGAGTCC
    CTCTGTGATG ACCTTGTCTC CGTGCTCACT GTTCTGTGTG AGAAGCTCCA AGCTGCCATC
    AATGACAGCC AGCAGCTGCA GCTCCTGTAC CTGGAGTGCA TCCTGTCCGT GCTCAGCAGC
    TCCTCCTCCT CCATGCAGCT GCACCGAGGC TTCACAGACC TCATCTGGAA GAACCTTTGC
    CCCGCTCTCA TCGTGATCCT GGGGAATCCG ATTCATGATA AAACCATCAC CTCTGCACAC
    AGCAGCAGCA CGAGTACCAG CATCGAGTCA GACTCTGTGT CTCCTGGGGT TTCCGACCAC
    GGCCGGGGTT CGGGCTGCTC CTCCACAGCG CCAGCCCTGA GTGGGCCAGT GGCCCGGACC
    ATCTACTACA TCGCGGCTGA GCTGGTGCGG CTGGTGGGGT CGGTGGACTC CATGAAGCCC
    GTGTTGCAGT CCCTCTACCA CCGTGTGCTG CTCTACCCCC CACCCCAGCA CCGTGTGGAA
    GCCATCAAGA TCATGAAAGA GCTACTTGGG AGCCCAAAGC GTCTCTGTGA TTTGGCAGGA
    CCCAGCTCCA CTGAACCTGA CTCGAGAAAA AGATCAATTT CAAAAAGAAA GTCTCATCTG
    GATCTTCTCA AACTCATCAT GGACGGCATG ACCGAGGCGT GTATCAAGGG CGGCATCGAG
    GCCTGCTACG CCGCCGTGTC CTGCGTCTGC ACCTTGCTGG GAGCCTTGGA GGAGCTCAGC
    CAGGGCAGGG GCTTGAGCGA AAGTCAAGTC CAGCTGCTGC TGCTGCGCCT GGAGGAGCTG
    AAGGACGGGG CCGAGTCCAG CCGCGACTCC ATGGAGATCA ATGAGGCCGA CTTCCGCTGG
    CAGCGGCGAG TCCTGTCCTC GGAACACACA CCCTGGGAGT TGGGGAATGA GCGGAGCCTT
    GACATCAGCA TCAGCGTTAC CACGGACACG GGCCAGACCA CCTTGGAGGG AGAGTTGGGT
    CAGACCACAC CCGAGGACCA CTCGGAAAAC CCCAAGAATG GCCTCAAGTC ACCAGCCGTC
    CAGGAGGGCA AGGAGACTCT GAGTAAAGTG TCAGAACCAG AAGCTGTGGA CCAGCCAGAC
    GTGGTTCAAA GGAGCCACAC GGTTGCCTAC CCCGATATAA CCAACTTCCT GTCCGTCGAC
    TGCAGGATGA GGTCCTACGG ATCCAGATAC AGTGAGAGCA ACTTCAGCGT GGACGACCAA
    GACCTCTCTC GGACAGAATT CGACTCCTGT GACCAGTACT CCATGGCGGC TGAAAAGGAC
    TCCGGGAGAT CGGACGTGTC GGACATTGGA TCCGACAACT GTTCTTTAGC TGATGAGGAG
    CAGACCCCCC GGGACTGCCT GGGCCACAGG TCCCTGCGGA CTGCAGCTCT GTCTCTCAAG
    CTGCTGAAGA ACCAGGAGGC TGACCAGCAC AGCGCCCGGC TGTTCGTGCA GTCGCTGGAG
    GGTCTCCTGC CGCGCCTCCT GGCCCTGCCC AGTGTGGAAG AGGTGGACTC AGCCCTCCAG
    AACTTTGTCT CTACCTTCTG CTCAGGTATG ATGCATTCAC CAGGGTTTGA TGGGAACAGC
    AGCCTGGGCC TGCAGATGCT GATGAACGCG GACAGCCTGT ACTCGGCGGC GCACTGCGCG
    CTGCTGCTCA ACCTGAAGCT CTCCCACGGT GACTACTACC GCAAGCGGCC CACCCTAGCA
    CCCAGCACCA TGAAAGAGTT CATGAAGCAG GTCCAGACCA GCGGGGTGCT GATGGTCTTC
    TCCCAGGCCT GGATTGAGGA ACTTTACCAC CAGGTTCTTG ACAGAAACAT GCTCGGAGAA
    GCCGGCTACT GGGGCAGCCC TGAAGACAAC AGCCTCCCCC TCATCACCAT GCTGACGGAT
    ATCGATGGCT TAGAGAGCAG TGCAATTGGA GGGCAGCTGA TGGCCTCAGC TTCGATGGAG
    TCTCCTTTCA CCCAGGGCAG GAGAATTGAT GACTCCACGG TGGCAGGTGT GGCATTTGCT
    CGCTATATTC TGATTGGCTG CTGGAAGAAC TTGATCGACA CTTTATCAAC CCCCCTGACG
    GGCCGAATGG CCGGCAGCTC CAAAGGGCTG GCCTTCATCT TGGGAGCTGA AGGCATCAAA
    GAACAGAACC AAAAAGAACG CGATGCTATC TGCATGAGCC TCGACGGGCT ACGGAAAGCC
    GCGCGTCTGA GCTGCGCTCT AGGAGTTGCA GCCAACTGTG CTTCGGCCCT CGCCCAGATG
    GCAGCTGCCT CCTGTGTCCA GGAGGAGAAA GAGGAAAAGG AAACCCAGGA ACCCAGTGAT
    GCCATAGCAC AAGTGAAACT AAAAGTGGAG CAGAAGCTGG AGCAGATCGG GAAGCTGCAG
    GGGGTGTGGC TGCACACAGC CCACGTCCTG TGCATGGACG CCATCCTCAG TGTCGGCCTG
    GAGATGGGCA GCCACAACCC CGACTGCTGG CCACACGTGT TCAGGGTGTG CGAGTACGTG
    GGCACCTTGG AACACACCCA CTTCAGCGAT GGCACCTCTC AGCCCCCGCT GACCATCAGC
    CAGCCCCAGA AGGCCCCCAG AGGCGGACTC CTTGGGGACC TGGAGAGTGA GGGCTCAGCC
    CAGGAGCAGA GCCTGGAGCA GGAGAGCTCC CTGAGCGCGG CCCCCGTCAT CCAACCGCTC
    TCCATCCAGG AGCTCGTCCG AGAAGGCAGC CGAGGCCGGG CCTCTGACTT CCGTGGGGGC
    AGCCTCATGA CAGGCAGCAG TGCCGCCAAG GTGGTGCTCA CGCTCTCCAC GCAGGCTGAC
    AGGCTCTTTG AAGATGCTAC AGATAAACTG AACCTCATGG CTCTGGGAGG TTTTCTTTAC
    CAGCTGAAGA AAGCATCGCA GTCCCAGCTT TTCCATTCTG TTACGGAAAC AGTTGATTAC
    TCGCTGGCAA TGCCAGGAGA AGTGAAGTCT ACCCAAGACC GCAAAAGTGC CCTCCACCTG
    TTCCGCCTCG GGGACGCCAT GCTAAGGATC GTGAGGAGCA AATCGAGGCC CCTGCTCCAC
    GTGATGCGCT GCTGGAGCCT CGTGGCCCCA CACCTGGTAG AGGCTGCCTG CCATAAGGAA
    AGACACGTGT CTCAGAAGGC GGTTTCCTTC ATCCATGACA TACTGACGGA GGTTCTCACG
    GACTGGAATG AGCCACCTCA TTTCCACTTT AACGAAGCTC TCTTCCGACC GTTCGAACGT
    ATCATGCAGC TGGAGTTGTG TGACGAGGAC GTCCAAGACC AGGTTGTCAC GTCCATCGGG
    GAGCTGGTTG AAGTGTGCTC TGCCCAGATC CAGTCAGGCT GGAGGCCCCT GTTCAGCGCC
    CTGGAAACGG TGCACAGCGG GAACAAGTCC GAGGTGAAGG AGTACCTGGT GGGCGACTAT
    TCCATGGGAA AAGGCCAGGC TCCAGTGTTT GATGTATTTG AAGCTTTTCT CAATACCGAC
    AACATCCAGG TCTTTGCTAA TGCAGCCACT AGTTACATCA TGTGCCTTAT GAAGTTTGTC
    AAAGGACTGG GGGAGGTGGA CTGTAAGGAG ATTGGAGACT GTGTCCCAGG ACCCGGAGCC
    GCGTCCCCAG ACCTGTGCCT CCCCGCCCTG GACTACCTCC GGCGCTGTTC TCAGTTGTTG
    GCCAAAATCT ACAAAATGCC CTTGAAGCCA ATATTCCTTA GTGGGCGACT TGCTAGCTTG
    CCCCGAAGAC TTCAGGAGCA GTCAGCGAGC AGTGAGGATG GAATTGAGTC AGTCTTGTCT
    GACTTTGATG ACGACACTGG TCTGATCGAA GTTTGGATCA TCCTCTTGGA GCAGCTCACA
    GCAGCTGTGT CCAACTGTCC ACGTCAGCAC CAACCCCCCA CTCTGGATTT GCTCTTTGAG
    CTGTTGAGAG AGGTTACCAA AACACCTGGA CCAGGGTTTG GCATCTATGC AGTTGTTCAC
    CTTCTCCTTC CTGTGATGTC TCTTTGGCTT CGCCGTAGCC ATAAAGACCA TTCTTACTGG
    GACATTGCCT CGGCTAACTT CAAGCATGCC ATTGGTCTGT CCTGTGAGCT GGTGGTGGAG
    CACATTCAGA GCTTTATCCA CTCAGACATC AGGTATGAGA GCATGATCAA CACCATGCTG
    AAGGACCTCT TTGAGCTACT GGTAGCATGT GTAGCCAAGC CCACAGAAAC CATCTCCCGA
    GTAGGCTGCT CCTGTATTAG GTACGTCCTC GTGACAGCGG GCCCCGTGTT CACGGAGGAG
    ATGTGGAGGC TGGCCTGCTG TGCCCTGCAG GACGCCTTCT CGGCCACACT CAAGCCGGTG
    AAGGACCTGC TGGGCTGCTT CCACAGTGGC ACCGAGAGCT TCAGCGGCGA AGGCTGCCAG
    GTGAGGGTGG CCGCCCCGTC CTCCTCCCCG AGTGCCGAGG CCGAGTACTG GCGCATCCGA
    GCCATGGCTC AGCAGGTATT TATGTTGGAT ACCCAGTGCT CACCGAAGAC TCCAAACAAC
    TTCGATCATG CTCAGTCCTG TCAGCTCATT ATTGAACTGC CTCCTGATGA GAAGCCAAAT
    GGGCATACCA AGAAAAGTGT TTCTTTCAGG GAAATTGTGG TGAGTTTACT ATCCCATCAA
    GTATTACTCC AGAACTTATA TGATATCTTA TTAGAAGAGT TTGTCAAAGG CCCCTCTCCT
    GGGGAGGAGA AGATCCAAGG GCCAGAAACC AAGCTGGCTG GCTTCCTCAG ATACATCTCC
    ATGCAGAACC TGGCCGTCAT CTTTGACCTT CTGCTGGACT CCTACAGGAC GGCCAGAGAG
    TTTGACACCA GCCCAGGGCT GAAGTGCCTG CTGAAGAAAG TGTCTGGCAT AGGGGGCGCT
    GCCAACCTCT ACCGCCAGTC TGCCATGAGT TTCAACATTT ATTTCCACGC ACTGGTCTGT
    GCTGTTCTTA GCAACCAAGA AACCATCACT GCTGACCAAG TGAAGAAGGT CCTTTTTGAG
    GACGATGAGA GGAGCACAGA TTCGTCCCAG CAGTGTTCAT CAGAAGATGA AGACATTTTT
    GAGGAGACTG CCCAGGTGAG CCCCCCGAGA GGCAAAGAAA AGAGGCAGTG GAGGGCTCGG
    ATGCCCTCGC TCAGCGTCCA GCCCGTCAGC AACGCGGACT GGGTGTGGCT GGTCAAGAGG
    CTTCACAAGC TGTGCATGGA GCTGTGTAAC AACTACATCC AGATGCACCT GGACCTGGAA
    AACAGTATGG AGGAGGCCCC AATCTTCAAG AGTGACCCTT TCTTCATCCT TCCCTCCTTC
    CAGTCGGAGT CCTCCACCCC TTCCACCGGG GGCTTCTCAG GGAAAGACAC GCCTTCCGAG
    GATGACCGCA GTCAGACCCG GGATCATGCG GGCGAGCCTC TGAGCCTGAG GGGGACCAGC
    GGGGAGCTGC TGCTGCTGCC CCCGAGCCCC AAGGTGGAGA AGAAGGATCC CAGCCGCAAG
    AAGGAGTGGT GGGAGAACGC GGGCAACAAA ATCTATACCA TGGCAGCCGA CAAGACCATC
    TCAAAGCTGA TGACCGAGTA CAAGAGGAGG AAACAGCAGC ACAACCTGCC TGTCTTCCCC
    AAAGAGATCA AGGTGGAGAA GAAGGGAGAG GTGCTGGGCG CCAGGGGCCA AGGCTCCCCA
    CTGCTCCAGC GACCCCAGCA CCTGATGGAC CAGGGGCAGA TGCGGCACTC TTTCAGCGCC
    GGCCCTGAGC TGCTGCGCCA GGAGAAGAGG CCCCGCTCCG GCTCCACCGG CAGCTCCCGG
    AGTGTCTCCG CGAGGGACGC AGAAGCCCAG ATCCAGGCAT GGACCAACAT GGTGCTAACA
    GTCCTCAATC AGATTCAGAT CCTCCCAGAC CAGACCTTCA CAGCCCTCCA GCCAGCAGTG
    TTTCCGTGTA TCAGCCAGCT CACCTGTCAC GTGACTGACA TCAGAGTTCG CCAGGCTGTG
    AGAGAATGGC TCGGCAGAGT GGGCCGTGTC TATGACATCA TTATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211050.1
    CDS181..6588
    Translation

    Target ORF information:

    RefSeq Version XM_005210993.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210993.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    ATAATTGAAA ACCCGGATGT CCCCCAGGAA TTCAGGAGTC AAGGGTCAAC AGTAGAGTCC
    CTCTGTGATG ACCTTGTCTC CGTGCTCACT GTTCTGTGTG AGAAGCTCCA AGCTGCCATC
    AATGACAGCC AGCAGCTGCA GCTCCTGTAC CTGGAGTGCA TCCTGTCCGT GCTCAGCAGC
    TCCTCCTCCT CCATGCAGCT GCACCGAGGC TTCACAGACC TCATCTGGAA GAACCTTTGC
    CCCGCTCTCA TCGTGATCCT GGGGAATCCG ATTCATGATA AAACCATCAC CTCTGCACAC
    AGCAGCAGCA CGAGTACCAG CATCGAGTCA GACTCTGTGT CTCCTGGGGT TTCCGACCAC
    GGCCGGGGTT CGGGCTGCTC CTCCACAGCG CCAGCCCTGA GTGGGCCAGT GGCCCGGACC
    ATCTACTACA TCGCGGCTGA GCTGGTGCGG CTGGTGGGGT CGGTGGACTC CATGAAGCCC
    GTGTTGCAGT CCCTCTACCA CCGTGTGCTG CTCTACCCCC CACCCCAGCA CCGTGTGGAA
    GCCATCAAGA TCATGAAAGA GCTACTTGGG AGCCCAAAGC GTCTCTGTGA TTTGGCAGGA
    CCCAGCTCCA CTGAACCTGA CTCGAGAAAA AGATCAATTT CAAAAAGAAA GTCTCATCTG
    GATCTTCTCA AACTCATCAT GGACGGCATG ACCGAGGCGT GTATCAAGGG CGGCATCGAG
    GCCTGCTACG CCGCCGTGTC CTGCGTCTGC ACCTTGCTGG GAGCCTTGGA GGAGCTCAGC
    CAGGGCAGGG GCTTGAGCGA AAGTCAAGTC CAGCTGCTGC TGCTGCGCCT GGAGGAGCTG
    AAGGACGGGG CCGAGTCCAG CCGCGACTCC ATGGAGATCA ATGAGGCCGA CTTCCGCTGG
    CAGCGGCGAG TCCTGTCCTC GGAACACACA CCCTGGGAGT TGGGGAATGA GCGGAGCCTT
    GACATCAGCA TCAGCGTTAC CACGGACACG GGCCAGACCA CCTTGGAGGG AGAGTTGGGT
    CAGACCACAC CCGAGGACCA CTCGGAAAAC CCCAAGAATG GCCTCAAGTC ACCAGCCGTC
    CAGGAGGGCA AGGAGACTCT GAGTAAAGTG TCAGAACCAG AAGCTGTGGA CCAGCCAGAC
    GTGGTTCAAA GGAGCCACAC GGTTGCCTAC CCCGATATAA CCAACTTCCT GTCCGTCGAC
    TGCAGGATGA GGTCCTACGG ATCCAGATAC AGTGAGAGCA ACTTCAGCGT GGACGACCAA
    GACCTCTCTC GGACAGAATT CGACTCCTGT GACCAGTACT CCATGGCGGC TGAAAAGGAC
    TCCGGGAGAT CGGACGTGTC GGACATTGGA TCCGACAACT GTTCTTTAGC TGATGAGGAG
    CAGACCCCCC GGGACTGCCT GGGCCACAGG TCCCTGCGGA CTGCAGCTCT GTCTCTCAAG
    CTGCTGAAGA ACCAGGAGGC TGACCAGCAC AGCGCCCGGC TGTTCGTGCA GTCGCTGGAG
    GGTCTCCTGC CGCGCCTCCT GGCCCTGCCC AGTGTGGAAG AGGTGGACTC AGCCCTCCAG
    AACTTTGTCT CTACCTTCTG CTCAGGTATG ATGCATTCAC CAGGGTTTGA TGGGAACAGC
    AGCCTGGGCC TGCAGATGCT GATGAACGCG GACAGCCTGT ACTCGGCGGC GCACTGCGCG
    CTGCTGCTCA ACCTGAAGCT CTCCCACGGT GACTACTACC GCAAGCGGCC CACCCTAGCA
    CCCAGCACCA TGAAAGAGTT CATGAAGCAG GTCCAGACCA GCGGGGTGCT GATGGTCTTC
    TCCCAGGCCT GGATTGAGGA ACTTTACCAC CAGGTTCTTG ACAGAAACAT GCTCGGAGAA
    GCCGGCTACT GGGGCAGCCC TGAAGACAAC AGCCTCCCCC TCATCACCAT GCTGACGGAT
    ATCGATGGCT TAGAGAGCAG TGCAATTGGA GGGCAGCTGA TGGCCTCAGC TTCGATGGAG
    TCTCCTTTCA CCCAGGGCAG GAGAATTGAT GACTCCACGG TGGCAGGTGT GGCATTTGCT
    CGCTATATTC TGATTGGCTG CTGGAAGAAC TTGATCGACA CTTTATCAAC CCCCCTGACG
    GGCCGAATGG CCGGCAGCTC CAAAGGGCTG GCCTTCATCT TGGGAGCTGA AGGCATCAAA
    GAACAGAACC AAAAAGAACG CGATGCTATC TGCATGAGCC TCGACGGGCT ACGGAAAGCC
    GCGCGTCTGA GCTGCGCTCT AGGAGTTGCA GCCAACTGTG CTTCGGCCCT CGCCCAGATG
    GCAGCTGCCT CCTGTGTCCA GGAGGAGAAA GAGGAAAAGG AAACCCAGGA ACCCAGTGAT
    GCCATAGCAC AAGTGAAACT AAAAGTGGAG CAGAAGCTGG AGCAGATCGG GAAGCTGCAG
    GGGGTGTGGC TGCACACAGC CCACGTCCTG TGCATGGACG CCATCCTCAG TGTCGGCCTG
    GAGATGGGCA GCCACAACCC CGACTGCTGG CCACACGTGT TCAGGGTGTG CGAGTACGTG
    GGCACCTTGG AACACACCCA CTTCAGCGAT GGCACCTCTC AGCCCCCGCT GACCATCAGC
    CAGCCCCAGA AGGCCCCCAG AGGCGGACTC CTTGGGGACC TGGAGAGTGA GGGCTCAGCC
    CAGGAGCAGA GCCTGGAGCA GGAGAGCTCC CTGAGCGCGG CCCCCGTCAT CCAACCGCTC
    TCCATCCAGG AGCTCGTCCG AGAAGGCAGC CGAGGCCGGG CCTCTGACTT CCGTGGGGGC
    AGCCTCATGA CAGGCAGCAG TGCCGCCAAG GTGGTGCTCA CGCTCTCCAC GCAGGCTGAC
    AGGCTCTTTG AAGATGCTAC AGATAAACTG AACCTCATGG CTCTGGGAGG TTTTCTTTAC
    CAGCTGAAGA AAGCATCGCA GTCCCAGCTT TTCCATTCTG TTACGGAAAC AGTTGATTAC
    TCGCTGGCAA TGCCAGGAGA AGTGAAGTCT ACCCAAGACC GCAAAAGTGC CCTCCACCTG
    TTCCGCCTCG GGGACGCCAT GCTAAGGATC GTGAGGAGCA AATCGAGGCC CCTGCTCCAC
    GTGATGCGCT GCTGGAGCCT CGTGGCCCCA CACCTGGTAG AGGCTGCCTG CCATAAGGAA
    AGACACGTGT CTCAGAAGGC GGTTTCCTTC ATCCATGACA TACTGACGGA GGTTCTCACG
    GACTGGAATG AGCCACCTCA TTTCCACTTT AACGAAGCTC TCTTCCGACC GTTCGAACGT
    ATCATGCAGC TGGAGTTGTG TGACGAGGAC GTCCAAGACC AGGTTGTCAC GTCCATCGGG
    GAGCTGGTTG AAGTGTGCTC TGCCCAGATC CAGTCAGGCT GGAGGCCCCT GTTCAGCGCC
    CTGGAAACGG TGCACAGCGG GAACAAGTCC GAGGTGAAGG AGTACCTGGT GGGCGACTAT
    TCCATGGGAA AAGGCCAGGC TCCAGTGTTT GATGTATTTG AAGCTTTTCT CAATACCGAC
    AACATCCAGG TCTTTGCTAA TGCAGCCACT AGTTACATCA TGTGCCTTAT GAAGTTTGTC
    AAAGGACTGG GGGAGGTGGA CTGTAAGGAG ATTGGAGACT GTGTCCCAGG ACCCGGAGCC
    GCGTCCCCAG ACCTGTGCCT CCCCGCCCTG GACTACCTCC GGCGCTGTTC TCAGTTGTTG
    GCCAAAATCT ACAAAATGCC CTTGAAGCCA ATATTCCTTA GTGGGCGACT TGCTAGCTTG
    CCCCGAAGAC TTCAGGAGCA GTCAGCGAGC AGTGAGGATG GAATTGAGTC AGTCTTGTCT
    GACTTTGATG ACGACACTGG TCTGATCGAA GTTTGGATCA TCCTCTTGGA GCAGCTCACA
    GCAGCTGTGT CCAACTGTCC ACGTCAGCAC CAACCCCCCA CTCTGGATTT GCTCTTTGAG
    CTGTTGAGAG AGGTTACCAA AACACCTGGA CCAGGGTTTG GCATCTATGC AGTTGTTCAC
    CTTCTCCTTC CTGTGATGTC TCTTTGGCTT CGCCGTAGCC ATAAAGACCA TTCTTACTGG
    GACATTGCCT CGGCTAACTT CAAGCATGCC ATTGGTCTGT CCTGTGAGCT GGTGGTGGAG
    CACATTCAGA GCTTTATCCA CTCAGACATC AGGTATGAGA GCATGATCAA CACCATGCTG
    AAGGACCTCT TTGAGCTACT GGTAGCATGT GTAGCCAAGC CCACAGAAAC CATCTCCCGA
    GTAGGCTGCT CCTGTATTAG GTACGTCCTC GTGACAGCGG GCCCCGTGTT CACGGAGGAG
    ATGTGGAGGC TGGCCTGCTG TGCCCTGCAG GACGCCTTCT CGGCCACACT CAAGCCGGTG
    AAGGACCTGC TGGGCTGCTT CCACAGTGGC ACCGAGAGCT TCAGCGGCGA AGGCTGCCAG
    GTGAGGGTGG CCGCCCCGTC CTCCTCCCCG AGTGCCGAGG CCGAGTACTG GCGCATCCGA
    GCCATGGCTC AGCAGGTATT TATGTTGGAT ACCCAGTGCT CACCGAAGAC TCCAAACAAC
    TTCGATCATG CTCAGTCCTG TCAGCTCATT ATTGAACTGC CTCCTGATGA GAAGCCAAAT
    GGGCATACCA AGAAAAGTGT TTCTTTCAGG GAAATTGTGG TGAGTTTACT ATCCCATCAA
    GTATTACTCC AGAACTTATA TGATATCTTA TTAGAAGAGT TTGTCAAAGG CCCCTCTCCT
    GGGGAGGAGA AGATCCAAGG GCCAGAAACC AAGCTGGCTG GCTTCCTCAG ATACATCTCC
    ATGCAGAACC TGGCCGTCAT CTTTGACCTT CTGCTGGACT CCTACAGGAC GGCCAGAGAG
    TTTGACACCA GCCCAGGGCT GAAGTGCCTG CTGAAGAAAG TGTCTGGCAT AGGGGGCGCT
    GCCAACCTCT ACCGCCAGTC TGCCATGAGT TTCAACATTT ATTTCCACGC ACTGGTCTGT
    GCTGTTCTTA GCAACCAAGA AACCATCACT GCTGACCAAG TGAAGAAGGT CCTTTTTGAG
    GACGATGAGA GGAGCACAGA TTCGTCCCAG CAGTGTTCAT CAGAAGATGA AGACATTTTT
    GAGGAGACTG CCCAGGTGAG CCCCCCGAGA GGCAAAGAAA AGAGGCAGTG GAGGGCTCGG
    ATGCCCTCGC TCAGCGTCCA GCCCGTCAGC AACGCGGACT GGGTGTGGCT GGTCAAGAGG
    CTTCACAAGC TGTGCATGGA GCTGTGTAAC AACTACATCC AGATGCACCT GGACCTGGAA
    AACAGTATGG AGGAGGCCCC AATCTTCAAG AGTGACCCTT TCTTCATCCT TCCCTCCTTC
    CAGTCGGAGT CCTCCACCCC TTCCACCGGG GGCTTCTCAG GGAAAGACAC GCCTTCCGAG
    GATGACCGCA GTCAGACCCG GGATCATGCG GGCGAGCCTC TGAGCCTGAG GGGGACCAGC
    GGGGAGCTGC TGCTGCTGCC CCCGAGCCCC AAGGTGGAGA AGAAGGATCC CAGCCGCAAG
    AAGGAGTGGT GGGAGAACGC GGGCAACAAA ATCTATACCA TGGCAGCCGA CAAGACCATC
    TCAAAGCTGA TGACCGAGTA CAAGAGGAGG AAACAGCAGC ACAACCTGCC TGTCTTCCCC
    AAAGAGATCA AGGTGGAGAA GAAGGGAGAG GTGCTGGGCG CCAGGGGCCA AGGCTCCCCA
    CTGCTCCAGC GACCCCAGCA CCTGATGGAC CAGGGGCAGA TGCGGCACTC TTTCAGCGCC
    GGCCCTGAGC TGCTGCGCCA GGAGAAGAGG CCCCGCTCCG GCTCCACCGG CAGCTCCCGG
    AGTGTCTCCG CGAGGGACGC AGAAGCCCAG ATCCAGGCAT GGACCAACAT GGTGCTAACA
    GTCCTCAATC AGATTCAGAT CCTCCCAGAC CAGACCTTCA CAGCCCTCCA GCCAGCAGTG
    TTTCCGTGTA TCAGCCAGCT CACCTGTCAC GTGACTGACA TCAGAGTTCG CCAGGCTGTG
    AGAGAATGGC TCGGCAGAGT GGGCCGTGTC TATGACATCA TTATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22722
    Clone ID Related Accession (Same CDS sequence) XM_005210993.3 , XM_005210993.4 , XM_015464838.1
    Accession Version XM_005210993.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210993.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    ATAATTGAAA ACCCGGATGT CCCCCAGGAA TTCAGGAGTC AAGGGTCAAC AGTAGAGTCC
    CTCTGTGATG ACCTTGTCTC CGTGCTCACT GTTCTGTGTG AGAAGCTCCA AGCTGCCATC
    AATGACAGCC AGCAGCTGCA GCTCCTGTAC CTGGAGTGCA TCCTGTCCGT GCTCAGCAGC
    TCCTCCTCCT CCATGCAGCT GCACCGAGGC TTCACAGACC TCATCTGGAA GAACCTTTGC
    CCCGCTCTCA TCGTGATCCT GGGGAATCCG ATTCATGATA AAACCATCAC CTCTGCACAC
    AGCAGCAGCA CGAGTACCAG CATCGAGTCA GACTCTGTGT CTCCTGGGGT TTCCGACCAC
    GGCCGGGGTT CGGGCTGCTC CTCCACAGCG CCAGCCCTGA GTGGGCCAGT GGCCCGGACC
    ATCTACTACA TCGCGGCTGA GCTGGTGCGG CTGGTGGGGT CGGTGGACTC CATGAAGCCC
    GTGTTGCAGT CCCTCTACCA CCGTGTGCTG CTCTACCCCC CACCCCAGCA CCGTGTGGAA
    GCCATCAAGA TCATGAAAGA GCTACTTGGG AGCCCAAAGC GTCTCTGTGA TTTGGCAGGA
    CCCAGCTCCA CTGAACCTGA CTCGAGAAAA AGATCAATTT CAAAAAGAAA GTCTCATCTG
    GATCTTCTCA AACTCATCAT GGACGGCATG ACCGAGGCGT GTATCAAGGG CGGCATCGAG
    GCCTGCTACG CCGCCGTGTC CTGCGTCTGC ACCTTGCTGG GAGCCTTGGA GGAGCTCAGC
    CAGGGCAGGG GCTTGAGCGA AAGTCAAGTC CAGCTGCTGC TGCTGCGCCT GGAGGAGCTG
    AAGGACGGGG CCGAGTCCAG CCGCGACTCC ATGGAGATCA ATGAGGCCGA CTTCCGCTGG
    CAGCGGCGAG TCCTGTCCTC GGAACACACA CCCTGGGAGT TGGGGAATGA GCGGAGCCTT
    GACATCAGCA TCAGCGTTAC CACGGACACG GGCCAGACCA CCTTGGAGGG AGAGTTGGGT
    CAGACCACAC CCGAGGACCA CTCGGAAAAC CCCAAGAATG GCCTCAAGTC ACCAGCCGTC
    CAGGAGGGCA AGGAGACTCT GAGTAAAGTG TCAGAACCAG AAGCTGTGGA CCAGCCAGAC
    GTGGTTCAAA GGAGCCACAC GGTTGCCTAC CCCGATATAA CCAACTTCCT GTCCGTCGAC
    TGCAGGATGA GGTCCTACGG ATCCAGATAC AGTGAGAGCA ACTTCAGCGT GGACGACCAA
    GACCTCTCTC GGACAGAATT CGACTCCTGT GACCAGTACT CCATGGCGGC TGAAAAGGAC
    TCCGGGAGAT CGGACGTGTC GGACATTGGA TCCGACAACT GTTCTTTAGC TGATGAGGAG
    CAGACCCCCC GGGACTGCCT GGGCCACAGG TCCCTGCGGA CTGCAGCTCT GTCTCTCAAG
    CTGCTGAAGA ACCAGGAGGC TGACCAGCAC AGCGCCCGGC TGTTCGTGCA GTCGCTGGAG
    GGTCTCCTGC CGCGCCTCCT GGCCCTGCCC AGTGTGGAAG AGGTGGACTC AGCCCTCCAG
    AACTTTGTCT CTACCTTCTG CTCAGGTATG ATGCATTCAC CAGGGTTTGA TGGGAACAGC
    AGCCTGGGCC TGCAGATGCT GATGAACGCG GACAGCCTGT ACTCGGCGGC GCACTGCGCG
    CTGCTGCTCA ACCTGAAGCT CTCCCACGGT GACTACTACC GCAAGCGGCC CACCCTAGCA
    CCCAGCACCA TGAAAGAGTT CATGAAGCAG GTCCAGACCA GCGGGGTGCT GATGGTCTTC
    TCCCAGGCCT GGATTGAGGA ACTTTACCAC CAGGTTCTTG ACAGAAACAT GCTCGGAGAA
    GCCGGCTACT GGGGCAGCCC TGAAGACAAC AGCCTCCCCC TCATCACCAT GCTGACGGAT
    ATCGATGGCT TAGAGAGCAG TGCAATTGGA GGGCAGCTGA TGGCCTCAGC TTCGATGGAG
    TCTCCTTTCA CCCAGGGCAG GAGAATTGAT GACTCCACGG TGGCAGGTGT GGCATTTGCT
    CGCTATATTC TGATTGGCTG CTGGAAGAAC TTGATCGACA CTTTATCAAC CCCCCTGACG
    GGCCGAATGG CCGGCAGCTC CAAAGGGCTG GCCTTCATCT TGGGAGCTGA AGGCATCAAA
    GAACAGAACC AAAAAGAACG CGATGCTATC TGCATGAGCC TCGACGGGCT ACGGAAAGCC
    GCGCGTCTGA GCTGCGCTCT AGGAGTTGCA GCCAACTGTG CTTCGGCCCT CGCCCAGATG
    GCAGCTGCCT CCTGTGTCCA GGAGGAGAAA GAGGAAAAGG AAACCCAGGA ACCCAGTGAT
    GCCATAGCAC AAGTGAAACT AAAAGTGGAG CAGAAGCTGG AGCAGATCGG GAAGCTGCAG
    GGGGTGTGGC TGCACACAGC CCACGTCCTG TGCATGGACG CCATCCTCAG TGTCGGCCTG
    GAGATGGGCA GCCACAACCC CGACTGCTGG CCACACGTGT TCAGGGTGTG CGAGTACGTG
    GGCACCTTGG AACACACCCA CTTCAGCGAT GGCACCTCTC AGCCCCCGCT GACCATCAGC
    CAGCCCCAGA AGGCCCCCAG AGGCGGACTC CTTGGGGACC TGGAGAGTGA GGGCTCAGCC
    CAGGAGCAGA GCCTGGAGCA GGAGAGCTCC CTGAGCGCGG CCCCCGTCAT CCAACCGCTC
    TCCATCCAGG AGCTCGTCCG AGAAGGCAGC CGAGGCCGGG CCTCTGACTT CCGTGGGGGC
    AGCCTCATGA CAGGCAGCAG TGCCGCCAAG GTGGTGCTCA CGCTCTCCAC GCAGGCTGAC
    AGGCTCTTTG AAGATGCTAC AGATAAACTG AACCTCATGG CTCTGGGAGG TTTTCTTTAC
    CAGCTGAAGA AAGCATCGCA GTCCCAGCTT TTCCATTCTG TTACGGAAAC AGTTGATTAC
    TCGCTGGCAA TGCCAGGAGA AGTGAAGTCT ACCCAAGACC GCAAAAGTGC CCTCCACCTG
    TTCCGCCTCG GGGACGCCAT GCTAAGGATC GTGAGGAGCA AATCGAGGCC CCTGCTCCAC
    GTGATGCGCT GCTGGAGCCT CGTGGCCCCA CACCTGGTAG AGGCTGCCTG CCATAAGGAA
    AGACACGTGT CTCAGAAGGC GGTTTCCTTC ATCCATGACA TACTGACGGA GGTTCTCACG
    GACTGGAATG AGCCACCTCA TTTCCACTTT AACGAAGCTC TCTTCCGACC GTTCGAACGT
    ATCATGCAGC TGGAGTTGTG TGACGAGGAC GTCCAAGACC AGGTTGTCAC GTCCATCGGG
    GAGCTGGTTG AAGTGTGCTC TGCCCAGATC CAGTCAGGCT GGAGGCCCCT GTTCAGCGCC
    CTGGAAACGG TGCACAGCGG GAACAAGTCC GAGGTGAAGG AGTACCTGGT GGGCGACTAT
    TCCATGGGAA AAGGCCAGGC TCCAGTGTTT GATGTATTTG AAGCTTTTCT CAATACCGAC
    AACATCCAGG TCTTTGCTAA TGCAGCCACT AGTTACATCA TGTGCCTTAT GAAGTTTGTC
    AAAGGACTGG GGGAGGTGGA CTGTAAGGAG ATTGGAGACT GTGTCCCAGG ACCCGGAGCC
    GCGTCCCCAG ACCTGTGCCT CCCCGCCCTG GACTACCTCC GGCGCTGTTC TCAGTTGTTG
    GCCAAAATCT ACAAAATGCC CTTGAAGCCA ATATTCCTTA GTGGGCGACT TGCTAGCTTG
    CCCCGAAGAC TTCAGGAGCA GTCAGCGAGC AGTGAGGATG GAATTGAGTC AGTCTTGTCT
    GACTTTGATG ACGACACTGG TCTGATCGAA GTTTGGATCA TCCTCTTGGA GCAGCTCACA
    GCAGCTGTGT CCAACTGTCC ACGTCAGCAC CAACCCCCCA CTCTGGATTT GCTCTTTGAG
    CTGTTGAGAG AGGTTACCAA AACACCTGGA CCAGGGTTTG GCATCTATGC AGTTGTTCAC
    CTTCTCCTTC CTGTGATGTC TCTTTGGCTT CGCCGTAGCC ATAAAGACCA TTCTTACTGG
    GACATTGCCT CGGCTAACTT CAAGCATGCC ATTGGTCTGT CCTGTGAGCT GGTGGTGGAG
    CACATTCAGA GCTTTATCCA CTCAGACATC AGGTATGAGA GCATGATCAA CACCATGCTG
    AAGGACCTCT TTGAGCTACT GGTAGCATGT GTAGCCAAGC CCACAGAAAC CATCTCCCGA
    GTAGGCTGCT CCTGTATTAG GTACGTCCTC GTGACAGCGG GCCCCGTGTT CACGGAGGAG
    ATGTGGAGGC TGGCCTGCTG TGCCCTGCAG GACGCCTTCT CGGCCACACT CAAGCCGGTG
    AAGGACCTGC TGGGCTGCTT CCACAGTGGC ACCGAGAGCT TCAGCGGCGA AGGCTGCCAG
    GTGAGGGTGG CCGCCCCGTC CTCCTCCCCG AGTGCCGAGG CCGAGTACTG GCGCATCCGA
    GCCATGGCTC AGCAGGTATT TATGTTGGAT ACCCAGTGCT CACCGAAGAC TCCAAACAAC
    TTCGATCATG CTCAGTCCTG TCAGCTCATT ATTGAACTGC CTCCTGATGA GAAGCCAAAT
    GGGCATACCA AGAAAAGTGT TTCTTTCAGG GAAATTGTGG TGAGTTTACT ATCCCATCAA
    GTATTACTCC AGAACTTATA TGATATCTTA TTAGAAGAGT TTGTCAAAGG CCCCTCTCCT
    GGGGAGGAGA AGATCCAAGG GCCAGAAACC AAGCTGGCTG GCTTCCTCAG ATACATCTCC
    ATGCAGAACC TGGCCGTCAT CTTTGACCTT CTGCTGGACT CCTACAGGAC GGCCAGAGAG
    TTTGACACCA GCCCAGGGCT GAAGTGCCTG CTGAAGAAAG TGTCTGGCAT AGGGGGCGCT
    GCCAACCTCT ACCGCCAGTC TGCCATGAGT TTCAACATTT ATTTCCACGC ACTGGTCTGT
    GCTGTTCTTA GCAACCAAGA AACCATCACT GCTGACCAAG TGAAGAAGGT CCTTTTTGAG
    GACGATGAGA GGAGCACAGA TTCGTCCCAG CAGTGTTCAT CAGAAGATGA AGACATTTTT
    GAGGAGACTG CCCAGGTGAG CCCCCCGAGA GGCAAAGAAA AGAGGCAGTG GAGGGCTCGG
    ATGCCCTCGC TCAGCGTCCA GCCCGTCAGC AACGCGGACT GGGTGTGGCT GGTCAAGAGG
    CTTCACAAGC TGTGCATGGA GCTGTGTAAC AACTACATCC AGATGCACCT GGACCTGGAA
    AACAGTATGG AGGAGGCCCC AATCTTCAAG AGTGACCCTT TCTTCATCCT TCCCTCCTTC
    CAGTCGGAGT CCTCCACCCC TTCCACCGGG GGCTTCTCAG GGAAAGACAC GCCTTCCGAG
    GATGACCGCA GTCAGACCCG GGATCATGCG GGCGAGCCTC TGAGCCTGAG GGGGACCAGC
    GGGGAGCTGC TGCTGCTGCC CCCGAGCCCC AAGGTGGAGA AGAAGGATCC CAGCCGCAAG
    AAGGAGTGGT GGGAGAACGC GGGCAACAAA ATCTATACCA TGGCAGCCGA CAAGACCATC
    TCAAAGCTGA TGACCGAGTA CAAGAGGAGG AAACAGCAGC ACAACCTGCC TGTCTTCCCC
    AAAGAGATCA AGGTGGAGAA GAAGGGAGAG GTGCTGGGCG CCAGGGGCCA AGGCTCCCCA
    CTGCTCCAGC GACCCCAGCA CCTGATGGAC CAGGGGCAGA TGCGGCACTC TTTCAGCGCC
    GGCCCTGAGC TGCTGCGCCA GGAGAAGAGG CCCCGCTCCG GCTCCACCGG CAGCTCCCGG
    AGTGTCTCCG CGAGGGACGC AGAAGCCCAG ATCCAGGCAT GGACCAACAT GGTGCTAACA
    GTCCTCAATC AGATTCAGAT CCTCCCAGAC CAGACCTTCA CAGCCCTCCA GCCAGCAGTG
    TTTCCGTGTA TCAGCCAGCT CACCTGTCAC GTGACTGACA TCAGAGTTCG CCAGGCTGTG
    AGAGAATGGC TCGGCAGAGT GGGCCGTGTC TATGACATCA TTATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211050.1
    CDS94..6501
    Translation

    Target ORF information:

    RefSeq Version XM_005210993.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210993.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    ATAATTGAAA ACCCGGATGT CCCCCAGGAA TTCAGGAGTC AAGGGTCAAC AGTAGAGTCC
    CTCTGTGATG ACCTTGTCTC CGTGCTCACT GTTCTGTGTG AGAAGCTCCA AGCTGCCATC
    AATGACAGCC AGCAGCTGCA GCTCCTGTAC CTGGAGTGCA TCCTGTCCGT GCTCAGCAGC
    TCCTCCTCCT CCATGCAGCT GCACCGAGGC TTCACAGACC TCATCTGGAA GAACCTTTGC
    CCCGCTCTCA TCGTGATCCT GGGGAATCCG ATTCATGATA AAACCATCAC CTCTGCACAC
    AGCAGCAGCA CGAGTACCAG CATCGAGTCA GACTCTGTGT CTCCTGGGGT TTCCGACCAC
    GGCCGGGGTT CGGGCTGCTC CTCCACAGCG CCAGCCCTGA GTGGGCCAGT GGCCCGGACC
    ATCTACTACA TCGCGGCTGA GCTGGTGCGG CTGGTGGGGT CGGTGGACTC CATGAAGCCC
    GTGTTGCAGT CCCTCTACCA CCGTGTGCTG CTCTACCCCC CACCCCAGCA CCGTGTGGAA
    GCCATCAAGA TCATGAAAGA GCTACTTGGG AGCCCAAAGC GTCTCTGTGA TTTGGCAGGA
    CCCAGCTCCA CTGAACCTGA CTCGAGAAAA AGATCAATTT CAAAAAGAAA GTCTCATCTG
    GATCTTCTCA AACTCATCAT GGACGGCATG ACCGAGGCGT GTATCAAGGG CGGCATCGAG
    GCCTGCTACG CCGCCGTGTC CTGCGTCTGC ACCTTGCTGG GAGCCTTGGA GGAGCTCAGC
    CAGGGCAGGG GCTTGAGCGA AAGTCAAGTC CAGCTGCTGC TGCTGCGCCT GGAGGAGCTG
    AAGGACGGGG CCGAGTCCAG CCGCGACTCC ATGGAGATCA ATGAGGCCGA CTTCCGCTGG
    CAGCGGCGAG TCCTGTCCTC GGAACACACA CCCTGGGAGT TGGGGAATGA GCGGAGCCTT
    GACATCAGCA TCAGCGTTAC CACGGACACG GGCCAGACCA CCTTGGAGGG AGAGTTGGGT
    CAGACCACAC CCGAGGACCA CTCGGAAAAC CCCAAGAATG GCCTCAAGTC ACCAGCCGTC
    CAGGAGGGCA AGGAGACTCT GAGTAAAGTG TCAGAACCAG AAGCTGTGGA CCAGCCAGAC
    GTGGTTCAAA GGAGCCACAC GGTTGCCTAC CCCGATATAA CCAACTTCCT GTCCGTCGAC
    TGCAGGATGA GGTCCTACGG ATCCAGATAC AGTGAGAGCA ACTTCAGCGT GGACGACCAA
    GACCTCTCTC GGACAGAATT CGACTCCTGT GACCAGTACT CCATGGCGGC TGAAAAGGAC
    TCCGGGAGAT CGGACGTGTC GGACATTGGA TCCGACAACT GTTCTTTAGC TGATGAGGAG
    CAGACCCCCC GGGACTGCCT GGGCCACAGG TCCCTGCGGA CTGCAGCTCT GTCTCTCAAG
    CTGCTGAAGA ACCAGGAGGC TGACCAGCAC AGCGCCCGGC TGTTCGTGCA GTCGCTGGAG
    GGTCTCCTGC CGCGCCTCCT GGCCCTGCCC AGTGTGGAAG AGGTGGACTC AGCCCTCCAG
    AACTTTGTCT CTACCTTCTG CTCAGGTATG ATGCATTCAC CAGGGTTTGA TGGGAACAGC
    AGCCTGGGCC TGCAGATGCT GATGAACGCG GACAGCCTGT ACTCGGCGGC GCACTGCGCG
    CTGCTGCTCA ACCTGAAGCT CTCCCACGGT GACTACTACC GCAAGCGGCC CACCCTAGCA
    CCCAGCACCA TGAAAGAGTT CATGAAGCAG GTCCAGACCA GCGGGGTGCT GATGGTCTTC
    TCCCAGGCCT GGATTGAGGA ACTTTACCAC CAGGTTCTTG ACAGAAACAT GCTCGGAGAA
    GCCGGCTACT GGGGCAGCCC TGAAGACAAC AGCCTCCCCC TCATCACCAT GCTGACGGAT
    ATCGATGGCT TAGAGAGCAG TGCAATTGGA GGGCAGCTGA TGGCCTCAGC TTCGATGGAG
    TCTCCTTTCA CCCAGGGCAG GAGAATTGAT GACTCCACGG TGGCAGGTGT GGCATTTGCT
    CGCTATATTC TGATTGGCTG CTGGAAGAAC TTGATCGACA CTTTATCAAC CCCCCTGACG
    GGCCGAATGG CCGGCAGCTC CAAAGGGCTG GCCTTCATCT TGGGAGCTGA AGGCATCAAA
    GAACAGAACC AAAAAGAACG CGATGCTATC TGCATGAGCC TCGACGGGCT ACGGAAAGCC
    GCGCGTCTGA GCTGCGCTCT AGGAGTTGCA GCCAACTGTG CTTCGGCCCT CGCCCAGATG
    GCAGCTGCCT CCTGTGTCCA GGAGGAGAAA GAGGAAAAGG AAACCCAGGA ACCCAGTGAT
    GCCATAGCAC AAGTGAAACT AAAAGTGGAG CAGAAGCTGG AGCAGATCGG GAAGCTGCAG
    GGGGTGTGGC TGCACACAGC CCACGTCCTG TGCATGGACG CCATCCTCAG TGTCGGCCTG
    GAGATGGGCA GCCACAACCC CGACTGCTGG CCACACGTGT TCAGGGTGTG CGAGTACGTG
    GGCACCTTGG AACACACCCA CTTCAGCGAT GGCACCTCTC AGCCCCCGCT GACCATCAGC
    CAGCCCCAGA AGGCCCCCAG AGGCGGACTC CTTGGGGACC TGGAGAGTGA GGGCTCAGCC
    CAGGAGCAGA GCCTGGAGCA GGAGAGCTCC CTGAGCGCGG CCCCCGTCAT CCAACCGCTC
    TCCATCCAGG AGCTCGTCCG AGAAGGCAGC CGAGGCCGGG CCTCTGACTT CCGTGGGGGC
    AGCCTCATGA CAGGCAGCAG TGCCGCCAAG GTGGTGCTCA CGCTCTCCAC GCAGGCTGAC
    AGGCTCTTTG AAGATGCTAC AGATAAACTG AACCTCATGG CTCTGGGAGG TTTTCTTTAC
    CAGCTGAAGA AAGCATCGCA GTCCCAGCTT TTCCATTCTG TTACGGAAAC AGTTGATTAC
    TCGCTGGCAA TGCCAGGAGA AGTGAAGTCT ACCCAAGACC GCAAAAGTGC CCTCCACCTG
    TTCCGCCTCG GGGACGCCAT GCTAAGGATC GTGAGGAGCA AATCGAGGCC CCTGCTCCAC
    GTGATGCGCT GCTGGAGCCT CGTGGCCCCA CACCTGGTAG AGGCTGCCTG CCATAAGGAA
    AGACACGTGT CTCAGAAGGC GGTTTCCTTC ATCCATGACA TACTGACGGA GGTTCTCACG
    GACTGGAATG AGCCACCTCA TTTCCACTTT AACGAAGCTC TCTTCCGACC GTTCGAACGT
    ATCATGCAGC TGGAGTTGTG TGACGAGGAC GTCCAAGACC AGGTTGTCAC GTCCATCGGG
    GAGCTGGTTG AAGTGTGCTC TGCCCAGATC CAGTCAGGCT GGAGGCCCCT GTTCAGCGCC
    CTGGAAACGG TGCACAGCGG GAACAAGTCC GAGGTGAAGG AGTACCTGGT GGGCGACTAT
    TCCATGGGAA AAGGCCAGGC TCCAGTGTTT GATGTATTTG AAGCTTTTCT CAATACCGAC
    AACATCCAGG TCTTTGCTAA TGCAGCCACT AGTTACATCA TGTGCCTTAT GAAGTTTGTC
    AAAGGACTGG GGGAGGTGGA CTGTAAGGAG ATTGGAGACT GTGTCCCAGG ACCCGGAGCC
    GCGTCCCCAG ACCTGTGCCT CCCCGCCCTG GACTACCTCC GGCGCTGTTC TCAGTTGTTG
    GCCAAAATCT ACAAAATGCC CTTGAAGCCA ATATTCCTTA GTGGGCGACT TGCTAGCTTG
    CCCCGAAGAC TTCAGGAGCA GTCAGCGAGC AGTGAGGATG GAATTGAGTC AGTCTTGTCT
    GACTTTGATG ACGACACTGG TCTGATCGAA GTTTGGATCA TCCTCTTGGA GCAGCTCACA
    GCAGCTGTGT CCAACTGTCC ACGTCAGCAC CAACCCCCCA CTCTGGATTT GCTCTTTGAG
    CTGTTGAGAG AGGTTACCAA AACACCTGGA CCAGGGTTTG GCATCTATGC AGTTGTTCAC
    CTTCTCCTTC CTGTGATGTC TCTTTGGCTT CGCCGTAGCC ATAAAGACCA TTCTTACTGG
    GACATTGCCT CGGCTAACTT CAAGCATGCC ATTGGTCTGT CCTGTGAGCT GGTGGTGGAG
    CACATTCAGA GCTTTATCCA CTCAGACATC AGGTATGAGA GCATGATCAA CACCATGCTG
    AAGGACCTCT TTGAGCTACT GGTAGCATGT GTAGCCAAGC CCACAGAAAC CATCTCCCGA
    GTAGGCTGCT CCTGTATTAG GTACGTCCTC GTGACAGCGG GCCCCGTGTT CACGGAGGAG
    ATGTGGAGGC TGGCCTGCTG TGCCCTGCAG GACGCCTTCT CGGCCACACT CAAGCCGGTG
    AAGGACCTGC TGGGCTGCTT CCACAGTGGC ACCGAGAGCT TCAGCGGCGA AGGCTGCCAG
    GTGAGGGTGG CCGCCCCGTC CTCCTCCCCG AGTGCCGAGG CCGAGTACTG GCGCATCCGA
    GCCATGGCTC AGCAGGTATT TATGTTGGAT ACCCAGTGCT CACCGAAGAC TCCAAACAAC
    TTCGATCATG CTCAGTCCTG TCAGCTCATT ATTGAACTGC CTCCTGATGA GAAGCCAAAT
    GGGCATACCA AGAAAAGTGT TTCTTTCAGG GAAATTGTGG TGAGTTTACT ATCCCATCAA
    GTATTACTCC AGAACTTATA TGATATCTTA TTAGAAGAGT TTGTCAAAGG CCCCTCTCCT
    GGGGAGGAGA AGATCCAAGG GCCAGAAACC AAGCTGGCTG GCTTCCTCAG ATACATCTCC
    ATGCAGAACC TGGCCGTCAT CTTTGACCTT CTGCTGGACT CCTACAGGAC GGCCAGAGAG
    TTTGACACCA GCCCAGGGCT GAAGTGCCTG CTGAAGAAAG TGTCTGGCAT AGGGGGCGCT
    GCCAACCTCT ACCGCCAGTC TGCCATGAGT TTCAACATTT ATTTCCACGC ACTGGTCTGT
    GCTGTTCTTA GCAACCAAGA AACCATCACT GCTGACCAAG TGAAGAAGGT CCTTTTTGAG
    GACGATGAGA GGAGCACAGA TTCGTCCCAG CAGTGTTCAT CAGAAGATGA AGACATTTTT
    GAGGAGACTG CCCAGGTGAG CCCCCCGAGA GGCAAAGAAA AGAGGCAGTG GAGGGCTCGG
    ATGCCCTCGC TCAGCGTCCA GCCCGTCAGC AACGCGGACT GGGTGTGGCT GGTCAAGAGG
    CTTCACAAGC TGTGCATGGA GCTGTGTAAC AACTACATCC AGATGCACCT GGACCTGGAA
    AACAGTATGG AGGAGGCCCC AATCTTCAAG AGTGACCCTT TCTTCATCCT TCCCTCCTTC
    CAGTCGGAGT CCTCCACCCC TTCCACCGGG GGCTTCTCAG GGAAAGACAC GCCTTCCGAG
    GATGACCGCA GTCAGACCCG GGATCATGCG GGCGAGCCTC TGAGCCTGAG GGGGACCAGC
    GGGGAGCTGC TGCTGCTGCC CCCGAGCCCC AAGGTGGAGA AGAAGGATCC CAGCCGCAAG
    AAGGAGTGGT GGGAGAACGC GGGCAACAAA ATCTATACCA TGGCAGCCGA CAAGACCATC
    TCAAAGCTGA TGACCGAGTA CAAGAGGAGG AAACAGCAGC ACAACCTGCC TGTCTTCCCC
    AAAGAGATCA AGGTGGAGAA GAAGGGAGAG GTGCTGGGCG CCAGGGGCCA AGGCTCCCCA
    CTGCTCCAGC GACCCCAGCA CCTGATGGAC CAGGGGCAGA TGCGGCACTC TTTCAGCGCC
    GGCCCTGAGC TGCTGCGCCA GGAGAAGAGG CCCCGCTCCG GCTCCACCGG CAGCTCCCGG
    AGTGTCTCCG CGAGGGACGC AGAAGCCCAG ATCCAGGCAT GGACCAACAT GGTGCTAACA
    GTCCTCAATC AGATTCAGAT CCTCCCAGAC CAGACCTTCA CAGCCCTCCA GCCAGCAGTG
    TTTCCGTGTA TCAGCCAGCT CACCTGTCAC GTGACTGACA TCAGAGTTCG CCAGGCTGTG
    AGAGAATGGC TCGGCAGAGT GGGCCGTGTC TATGACATCA TTATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22722
    Clone ID Related Accession (Same CDS sequence) XM_005210993.3 , XM_005210993.4 , XM_015464838.1
    Accession Version XM_015464838.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645711.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    ATAATTGAAA ACCCGGATGT CCCCCAGGAA TTCAGGAGTC AAGGGTCAAC AGTAGAGTCC
    CTCTGTGATG ACCTTGTCTC CGTGCTCACT GTTCTGTGTG AGAAGCTCCA AGCTGCCATC
    AATGACAGCC AGCAGCTGCA GCTCCTGTAC CTGGAGTGCA TCCTGTCCGT GCTCAGCAGC
    TCCTCCTCCT CCATGCAGCT GCACCGAGGC TTCACAGACC TCATCTGGAA GAACCTTTGC
    CCCGCTCTCA TCGTGATCCT GGGGAATCCG ATTCATGATA AAACCATCAC CTCTGCACAC
    AGCAGCAGCA CGAGTACCAG CATCGAGTCA GACTCTGTGT CTCCTGGGGT TTCCGACCAC
    GGCCGGGGTT CGGGCTGCTC CTCCACAGCG CCAGCCCTGA GTGGGCCAGT GGCCCGGACC
    ATCTACTACA TCGCGGCTGA GCTGGTGCGG CTGGTGGGGT CGGTGGACTC CATGAAGCCC
    GTGTTGCAGT CCCTCTACCA CCGTGTGCTG CTCTACCCCC CACCCCAGCA CCGTGTGGAA
    GCCATCAAGA TCATGAAAGA GCTACTTGGG AGCCCAAAGC GTCTCTGTGA TTTGGCAGGA
    CCCAGCTCCA CTGAACCTGA CTCGAGAAAA AGATCAATTT CAAAAAGAAA GTCTCATCTG
    GATCTTCTCA AACTCATCAT GGACGGCATG ACCGAGGCGT GTATCAAGGG CGGCATCGAG
    GCCTGCTACG CCGCCGTGTC CTGCGTCTGC ACCTTGCTGG GAGCCTTGGA GGAGCTCAGC
    CAGGGCAGGG GCTTGAGCGA AAGTCAAGTC CAGCTGCTGC TGCTGCGCCT GGAGGAGCTG
    AAGGACGGGG CCGAGTCCAG CCGCGACTCC ATGGAGATCA ATGAGGCCGA CTTCCGCTGG
    CAGCGGCGAG TCCTGTCCTC GGAACACACA CCCTGGGAGT TGGGGAATGA GCGGAGCCTT
    GACATCAGCA TCAGCGTTAC CACGGACACG GGCCAGACCA CCTTGGAGGG AGAGTTGGGT
    CAGACCACAC CCGAGGACCA CTCGGAAAAC CCCAAGAATG GCCTCAAGTC ACCAGCCGTC
    CAGGAGGGCA AGGAGACTCT GAGTAAAGTG TCAGAACCAG AAGCTGTGGA CCAGCCAGAC
    GTGGTTCAAA GGAGCCACAC GGTTGCCTAC CCCGATATAA CCAACTTCCT GTCCGTCGAC
    TGCAGGATGA GGTCCTACGG ATCCAGATAC AGTGAGAGCA ACTTCAGCGT GGACGACCAA
    GACCTCTCTC GGACAGAATT CGACTCCTGT GACCAGTACT CCATGGCGGC TGAAAAGGAC
    TCCGGGAGAT CGGACGTGTC GGACATTGGA TCCGACAACT GTTCTTTAGC TGATGAGGAG
    CAGACCCCCC GGGACTGCCT GGGCCACAGG TCCCTGCGGA CTGCAGCTCT GTCTCTCAAG
    CTGCTGAAGA ACCAGGAGGC TGACCAGCAC AGCGCCCGGC TGTTCGTGCA GTCGCTGGAG
    GGTCTCCTGC CGCGCCTCCT GGCCCTGCCC AGTGTGGAAG AGGTGGACTC AGCCCTCCAG
    AACTTTGTCT CTACCTTCTG CTCAGGTATG ATGCATTCAC CAGGGTTTGA TGGGAACAGC
    AGCCTGGGCC TGCAGATGCT GATGAACGCG GACAGCCTGT ACTCGGCGGC GCACTGCGCG
    CTGCTGCTCA ACCTGAAGCT CTCCCACGGT GACTACTACC GCAAGCGGCC CACCCTAGCA
    CCCAGCACCA TGAAAGAGTT CATGAAGCAG GTCCAGACCA GCGGGGTGCT GATGGTCTTC
    TCCCAGGCCT GGATTGAGGA ACTTTACCAC CAGGTTCTTG ACAGAAACAT GCTCGGAGAA
    GCCGGCTACT GGGGCAGCCC TGAAGACAAC AGCCTCCCCC TCATCACCAT GCTGACGGAT
    ATCGATGGCT TAGAGAGCAG TGCAATTGGA GGGCAGCTGA TGGCCTCAGC TTCGATGGAG
    TCTCCTTTCA CCCAGGGCAG GAGAATTGAT GACTCCACGG TGGCAGGTGT GGCATTTGCT
    CGCTATATTC TGATTGGCTG CTGGAAGAAC TTGATCGACA CTTTATCAAC CCCCCTGACG
    GGCCGAATGG CCGGCAGCTC CAAAGGGCTG GCCTTCATCT TGGGAGCTGA AGGCATCAAA
    GAACAGAACC AAAAAGAACG CGATGCTATC TGCATGAGCC TCGACGGGCT ACGGAAAGCC
    GCGCGTCTGA GCTGCGCTCT AGGAGTTGCA GCCAACTGTG CTTCGGCCCT CGCCCAGATG
    GCAGCTGCCT CCTGTGTCCA GGAGGAGAAA GAGGAAAAGG AAACCCAGGA ACCCAGTGAT
    GCCATAGCAC AAGTGAAACT AAAAGTGGAG CAGAAGCTGG AGCAGATCGG GAAGCTGCAG
    GGGGTGTGGC TGCACACAGC CCACGTCCTG TGCATGGACG CCATCCTCAG TGTCGGCCTG
    GAGATGGGCA GCCACAACCC CGACTGCTGG CCACACGTGT TCAGGGTGTG CGAGTACGTG
    GGCACCTTGG AACACACCCA CTTCAGCGAT GGCACCTCTC AGCCCCCGCT GACCATCAGC
    CAGCCCCAGA AGGCCCCCAG AGGCGGACTC CTTGGGGACC TGGAGAGTGA GGGCTCAGCC
    CAGGAGCAGA GCCTGGAGCA GGAGAGCTCC CTGAGCGCGG CCCCCGTCAT CCAACCGCTC
    TCCATCCAGG AGCTCGTCCG AGAAGGCAGC CGAGGCCGGG CCTCTGACTT CCGTGGGGGC
    AGCCTCATGA CAGGCAGCAG TGCCGCCAAG GTGGTGCTCA CGCTCTCCAC GCAGGCTGAC
    AGGCTCTTTG AAGATGCTAC AGATAAACTG AACCTCATGG CTCTGGGAGG TTTTCTTTAC
    CAGCTGAAGA AAGCATCGCA GTCCCAGCTT TTCCATTCTG TTACGGAAAC AGTTGATTAC
    TCGCTGGCAA TGCCAGGAGA AGTGAAGTCT ACCCAAGACC GCAAAAGTGC CCTCCACCTG
    TTCCGCCTCG GGGACGCCAT GCTAAGGATC GTGAGGAGCA AATCGAGGCC CCTGCTCCAC
    GTGATGCGCT GCTGGAGCCT CGTGGCCCCA CACCTGGTAG AGGCTGCCTG CCATAAGGAA
    AGACACGTGT CTCAGAAGGC GGTTTCCTTC ATCCATGACA TACTGACGGA GGTTCTCACG
    GACTGGAATG AGCCACCTCA TTTCCACTTT AACGAAGCTC TCTTCCGACC GTTCGAACGT
    ATCATGCAGC TGGAGTTGTG TGACGAGGAC GTCCAAGACC AGGTTGTCAC GTCCATCGGG
    GAGCTGGTTG AAGTGTGCTC TGCCCAGATC CAGTCAGGCT GGAGGCCCCT GTTCAGCGCC
    CTGGAAACGG TGCACAGCGG GAACAAGTCC GAGGTGAAGG AGTACCTGGT GGGCGACTAT
    TCCATGGGAA AAGGCCAGGC TCCAGTGTTT GATGTATTTG AAGCTTTTCT CAATACCGAC
    AACATCCAGG TCTTTGCTAA TGCAGCCACT AGTTACATCA TGTGCCTTAT GAAGTTTGTC
    AAAGGACTGG GGGAGGTGGA CTGTAAGGAG ATTGGAGACT GTGTCCCAGG ACCCGGAGCC
    GCGTCCCCAG ACCTGTGCCT CCCCGCCCTG GACTACCTCC GGCGCTGTTC TCAGTTGTTG
    GCCAAAATCT ACAAAATGCC CTTGAAGCCA ATATTCCTTA GTGGGCGACT TGCTAGCTTG
    CCCCGAAGAC TTCAGGAGCA GTCAGCGAGC AGTGAGGATG GAATTGAGTC AGTCTTGTCT
    GACTTTGATG ACGACACTGG TCTGATCGAA GTTTGGATCA TCCTCTTGGA GCAGCTCACA
    GCAGCTGTGT CCAACTGTCC ACGTCAGCAC CAACCCCCCA CTCTGGATTT GCTCTTTGAG
    CTGTTGAGAG AGGTTACCAA AACACCTGGA CCAGGGTTTG GCATCTATGC AGTTGTTCAC
    CTTCTCCTTC CTGTGATGTC TCTTTGGCTT CGCCGTAGCC ATAAAGACCA TTCTTACTGG
    GACATTGCCT CGGCTAACTT CAAGCATGCC ATTGGTCTGT CCTGTGAGCT GGTGGTGGAG
    CACATTCAGA GCTTTATCCA CTCAGACATC AGGTATGAGA GCATGATCAA CACCATGCTG
    AAGGACCTCT TTGAGCTACT GGTAGCATGT GTAGCCAAGC CCACAGAAAC CATCTCCCGA
    GTAGGCTGCT CCTGTATTAG GTACGTCCTC GTGACAGCGG GCCCCGTGTT CACGGAGGAG
    ATGTGGAGGC TGGCCTGCTG TGCCCTGCAG GACGCCTTCT CGGCCACACT CAAGCCGGTG
    AAGGACCTGC TGGGCTGCTT CCACAGTGGC ACCGAGAGCT TCAGCGGCGA AGGCTGCCAG
    GTGAGGGTGG CCGCCCCGTC CTCCTCCCCG AGTGCCGAGG CCGAGTACTG GCGCATCCGA
    GCCATGGCTC AGCAGGTATT TATGTTGGAT ACCCAGTGCT CACCGAAGAC TCCAAACAAC
    TTCGATCATG CTCAGTCCTG TCAGCTCATT ATTGAACTGC CTCCTGATGA GAAGCCAAAT
    GGGCATACCA AGAAAAGTGT TTCTTTCAGG GAAATTGTGG TGAGTTTACT ATCCCATCAA
    GTATTACTCC AGAACTTATA TGATATCTTA TTAGAAGAGT TTGTCAAAGG CCCCTCTCCT
    GGGGAGGAGA AGATCCAAGG GCCAGAAACC AAGCTGGCTG GCTTCCTCAG ATACATCTCC
    ATGCAGAACC TGGCCGTCAT CTTTGACCTT CTGCTGGACT CCTACAGGAC GGCCAGAGAG
    TTTGACACCA GCCCAGGGCT GAAGTGCCTG CTGAAGAAAG TGTCTGGCAT AGGGGGCGCT
    GCCAACCTCT ACCGCCAGTC TGCCATGAGT TTCAACATTT ATTTCCACGC ACTGGTCTGT
    GCTGTTCTTA GCAACCAAGA AACCATCACT GCTGACCAAG TGAAGAAGGT CCTTTTTGAG
    GACGATGAGA GGAGCACAGA TTCGTCCCAG CAGTGTTCAT CAGAAGATGA AGACATTTTT
    GAGGAGACTG CCCAGGTGAG CCCCCCGAGA GGCAAAGAAA AGAGGCAGTG GAGGGCTCGG
    ATGCCCTCGC TCAGCGTCCA GCCCGTCAGC AACGCGGACT GGGTGTGGCT GGTCAAGAGG
    CTTCACAAGC TGTGCATGGA GCTGTGTAAC AACTACATCC AGATGCACCT GGACCTGGAA
    AACAGTATGG AGGAGGCCCC AATCTTCAAG AGTGACCCTT TCTTCATCCT TCCCTCCTTC
    CAGTCGGAGT CCTCCACCCC TTCCACCGGG GGCTTCTCAG GGAAAGACAC GCCTTCCGAG
    GATGACCGCA GTCAGACCCG GGATCATGCG GGCGAGCCTC TGAGCCTGAG GGGGACCAGC
    GGGGAGCTGC TGCTGCTGCC CCCGAGCCCC AAGGTGGAGA AGAAGGATCC CAGCCGCAAG
    AAGGAGTGGT GGGAGAACGC GGGCAACAAA ATCTATACCA TGGCAGCCGA CAAGACCATC
    TCAAAGCTGA TGACCGAGTA CAAGAGGAGG AAACAGCAGC ACAACCTGCC TGTCTTCCCC
    AAAGAGATCA AGGTGGAGAA GAAGGGAGAG GTGCTGGGCG CCAGGGGCCA AGGCTCCCCA
    CTGCTCCAGC GACCCCAGCA CCTGATGGAC CAGGGGCAGA TGCGGCACTC TTTCAGCGCC
    GGCCCTGAGC TGCTGCGCCA GGAGAAGAGG CCCCGCTCCG GCTCCACCGG CAGCTCCCGG
    AGTGTCTCCG CGAGGGACGC AGAAGCCCAG ATCCAGGCAT GGACCAACAT GGTGCTAACA
    GTCCTCAATC AGATTCAGAT CCTCCCAGAC CAGACCTTCA CAGCCCTCCA GCCAGCAGTG
    TTTCCGTGTA TCAGCCAGCT CACCTGTCAC GTGACTGACA TCAGAGTTCG CCAGGCTGTG
    AGAGAATGGC TCGGCAGAGT GGGCCGTGTC TATGACATCA TTATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320324.1
    CDS181..6588
    Translation

    Target ORF information:

    RefSeq Version XM_015464838.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ARFGEF family member 3 (ARFGEF3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464838.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGAAGAGA TCCTAAGGAA GCTGCAGAAG GAGGCGTCCG GGAGCAAGTA CAAAGCCATC 
    AAAGAGAGCT GCACCTGGGC CCTGGAAACC CTCGATGGTG TGGATACCAT TGTCAAGATC
    CCCCCACACT TGCTGAGGGA GAAATGCCTG CTGCCCCTCC AGTTGGCTTT GGAATCCAAG
    AATGTGAAGC TGGCTCAACA TGCCTTGGCA GGGATACAGA AGCTCCTGGC GGAAGAGAGG
    TTTGTATCCA TGGAAACGGA CTCTGATGAG AAGCAGCTGC TCAATCAGAT CCTGAACGCT
    GTGAAAGTGA CGCCTTCCCT CCACGAAGAC CTGCAGGTGG AAGTGATGAA GGTTTTACTC
    TGCATAACCT ACACTCCAAC CTTTGACCTG AATGGGAGTG CAGTGCTGAA GATCGCGGAG
    ATAATTGAAA ACCCGGATGT CCCCCAGGAA TTCAGGAGTC AAGGGTCAAC AGTAGAGTCC
    CTCTGTGATG ACCTTGTCTC CGTGCTCACT GTTCTGTGTG AGAAGCTCCA AGCTGCCATC
    AATGACAGCC AGCAGCTGCA GCTCCTGTAC CTGGAGTGCA TCCTGTCCGT GCTCAGCAGC
    TCCTCCTCCT CCATGCAGCT GCACCGAGGC TTCACAGACC TCATCTGGAA GAACCTTTGC
    CCCGCTCTCA TCGTGATCCT GGGGAATCCG ATTCATGATA AAACCATCAC CTCTGCACAC
    AGCAGCAGCA CGAGTACCAG CATCGAGTCA GACTCTGTGT CTCCTGGGGT TTCCGACCAC
    GGCCGGGGTT CGGGCTGCTC CTCCACAGCG CCAGCCCTGA GTGGGCCAGT GGCCCGGACC
    ATCTACTACA TCGCGGCTGA GCTGGTGCGG CTGGTGGGGT CGGTGGACTC CATGAAGCCC
    GTGTTGCAGT CCCTCTACCA CCGTGTGCTG CTCTACCCCC CACCCCAGCA CCGTGTGGAA
    GCCATCAAGA TCATGAAAGA GCTACTTGGG AGCCCAAAGC GTCTCTGTGA TTTGGCAGGA
    CCCAGCTCCA CTGAACCTGA CTCGAGAAAA AGATCAATTT CAAAAAGAAA GTCTCATCTG
    GATCTTCTCA AACTCATCAT GGACGGCATG ACCGAGGCGT GTATCAAGGG CGGCATCGAG
    GCCTGCTACG CCGCCGTGTC CTGCGTCTGC ACCTTGCTGG GAGCCTTGGA GGAGCTCAGC
    CAGGGCAGGG GCTTGAGCGA AAGTCAAGTC CAGCTGCTGC TGCTGCGCCT GGAGGAGCTG
    AAGGACGGGG CCGAGTCCAG CCGCGACTCC ATGGAGATCA ATGAGGCCGA CTTCCGCTGG
    CAGCGGCGAG TCCTGTCCTC GGAACACACA CCCTGGGAGT TGGGGAATGA GCGGAGCCTT
    GACATCAGCA TCAGCGTTAC CACGGACACG GGCCAGACCA CCTTGGAGGG AGAGTTGGGT
    CAGACCACAC CCGAGGACCA CTCGGAAAAC CCCAAGAATG GCCTCAAGTC ACCAGCCGTC
    CAGGAGGGCA AGGAGACTCT GAGTAAAGTG TCAGAACCAG AAGCTGTGGA CCAGCCAGAC
    GTGGTTCAAA GGAGCCACAC GGTTGCCTAC CCCGATATAA CCAACTTCCT GTCCGTCGAC
    TGCAGGATGA GGTCCTACGG ATCCAGATAC AGTGAGAGCA ACTTCAGCGT GGACGACCAA
    GACCTCTCTC GGACAGAATT CGACTCCTGT GACCAGTACT CCATGGCGGC TGAAAAGGAC
    TCCGGGAGAT CGGACGTGTC GGACATTGGA TCCGACAACT GTTCTTTAGC TGATGAGGAG
    CAGACCCCCC GGGACTGCCT GGGCCACAGG TCCCTGCGGA CTGCAGCTCT GTCTCTCAAG
    CTGCTGAAGA ACCAGGAGGC TGACCAGCAC AGCGCCCGGC TGTTCGTGCA GTCGCTGGAG
    GGTCTCCTGC CGCGCCTCCT GGCCCTGCCC AGTGTGGAAG AGGTGGACTC AGCCCTCCAG
    AACTTTGTCT CTACCTTCTG CTCAGGTATG ATGCATTCAC CAGGGTTTGA TGGGAACAGC
    AGCCTGGGCC TGCAGATGCT GATGAACGCG GACAGCCTGT ACTCGGCGGC GCACTGCGCG
    CTGCTGCTCA ACCTGAAGCT CTCCCACGGT GACTACTACC GCAAGCGGCC CACCCTAGCA
    CCCAGCACCA TGAAAGAGTT CATGAAGCAG GTCCAGACCA GCGGGGTGCT GATGGTCTTC
    TCCCAGGCCT GGATTGAGGA ACTTTACCAC CAGGTTCTTG ACAGAAACAT GCTCGGAGAA
    GCCGGCTACT GGGGCAGCCC TGAAGACAAC AGCCTCCCCC TCATCACCAT GCTGACGGAT
    ATCGATGGCT TAGAGAGCAG TGCAATTGGA GGGCAGCTGA TGGCCTCAGC TTCGATGGAG
    TCTCCTTTCA CCCAGGGCAG GAGAATTGAT GACTCCACGG TGGCAGGTGT GGCATTTGCT
    CGCTATATTC TGATTGGCTG CTGGAAGAAC TTGATCGACA CTTTATCAAC CCCCCTGACG
    GGCCGAATGG CCGGCAGCTC CAAAGGGCTG GCCTTCATCT TGGGAGCTGA AGGCATCAAA
    GAACAGAACC AAAAAGAACG CGATGCTATC TGCATGAGCC TCGACGGGCT ACGGAAAGCC
    GCGCGTCTGA GCTGCGCTCT AGGAGTTGCA GCCAACTGTG CTTCGGCCCT CGCCCAGATG
    GCAGCTGCCT CCTGTGTCCA GGAGGAGAAA GAGGAAAAGG AAACCCAGGA ACCCAGTGAT
    GCCATAGCAC AAGTGAAACT AAAAGTGGAG CAGAAGCTGG AGCAGATCGG GAAGCTGCAG
    GGGGTGTGGC TGCACACAGC CCACGTCCTG TGCATGGACG CCATCCTCAG TGTCGGCCTG
    GAGATGGGCA GCCACAACCC CGACTGCTGG CCACACGTGT TCAGGGTGTG CGAGTACGTG
    GGCACCTTGG AACACACCCA CTTCAGCGAT GGCACCTCTC AGCCCCCGCT GACCATCAGC
    CAGCCCCAGA AGGCCCCCAG AGGCGGACTC CTTGGGGACC TGGAGAGTGA GGGCTCAGCC
    CAGGAGCAGA GCCTGGAGCA GGAGAGCTCC CTGAGCGCGG CCCCCGTCAT CCAACCGCTC
    TCCATCCAGG AGCTCGTCCG AGAAGGCAGC CGAGGCCGGG CCTCTGACTT CCGTGGGGGC
    AGCCTCATGA CAGGCAGCAG TGCCGCCAAG GTGGTGCTCA CGCTCTCCAC GCAGGCTGAC
    AGGCTCTTTG AAGATGCTAC AGATAAACTG AACCTCATGG CTCTGGGAGG TTTTCTTTAC
    CAGCTGAAGA AAGCATCGCA GTCCCAGCTT TTCCATTCTG TTACGGAAAC AGTTGATTAC
    TCGCTGGCAA TGCCAGGAGA AGTGAAGTCT ACCCAAGACC GCAAAAGTGC CCTCCACCTG
    TTCCGCCTCG GGGACGCCAT GCTAAGGATC GTGAGGAGCA AATCGAGGCC CCTGCTCCAC
    GTGATGCGCT GCTGGAGCCT CGTGGCCCCA CACCTGGTAG AGGCTGCCTG CCATAAGGAA
    AGACACGTGT CTCAGAAGGC GGTTTCCTTC ATCCATGACA TACTGACGGA GGTTCTCACG
    GACTGGAATG AGCCACCTCA TTTCCACTTT AACGAAGCTC TCTTCCGACC GTTCGAACGT
    ATCATGCAGC TGGAGTTGTG TGACGAGGAC GTCCAAGACC AGGTTGTCAC GTCCATCGGG
    GAGCTGGTTG AAGTGTGCTC TGCCCAGATC CAGTCAGGCT GGAGGCCCCT GTTCAGCGCC
    CTGGAAACGG TGCACAGCGG GAACAAGTCC GAGGTGAAGG AGTACCTGGT GGGCGACTAT
    TCCATGGGAA AAGGCCAGGC TCCAGTGTTT GATGTATTTG AAGCTTTTCT CAATACCGAC
    AACATCCAGG TCTTTGCTAA TGCAGCCACT AGTTACATCA TGTGCCTTAT GAAGTTTGTC
    AAAGGACTGG GGGAGGTGGA CTGTAAGGAG ATTGGAGACT GTGTCCCAGG ACCCGGAGCC
    GCGTCCCCAG ACCTGTGCCT CCCCGCCCTG GACTACCTCC GGCGCTGTTC TCAGTTGTTG
    GCCAAAATCT ACAAAATGCC CTTGAAGCCA ATATTCCTTA GTGGGCGACT TGCTAGCTTG
    CCCCGAAGAC TTCAGGAGCA GTCAGCGAGC AGTGAGGATG GAATTGAGTC AGTCTTGTCT
    GACTTTGATG ACGACACTGG TCTGATCGAA GTTTGGATCA TCCTCTTGGA GCAGCTCACA
    GCAGCTGTGT CCAACTGTCC ACGTCAGCAC CAACCCCCCA CTCTGGATTT GCTCTTTGAG
    CTGTTGAGAG AGGTTACCAA AACACCTGGA CCAGGGTTTG GCATCTATGC AGTTGTTCAC
    CTTCTCCTTC CTGTGATGTC TCTTTGGCTT CGCCGTAGCC ATAAAGACCA TTCTTACTGG
    GACATTGCCT CGGCTAACTT CAAGCATGCC ATTGGTCTGT CCTGTGAGCT GGTGGTGGAG
    CACATTCAGA GCTTTATCCA CTCAGACATC AGGTATGAGA GCATGATCAA CACCATGCTG
    AAGGACCTCT TTGAGCTACT GGTAGCATGT GTAGCCAAGC CCACAGAAAC CATCTCCCGA
    GTAGGCTGCT CCTGTATTAG GTACGTCCTC GTGACAGCGG GCCCCGTGTT CACGGAGGAG
    ATGTGGAGGC TGGCCTGCTG TGCCCTGCAG GACGCCTTCT CGGCCACACT CAAGCCGGTG
    AAGGACCTGC TGGGCTGCTT CCACAGTGGC ACCGAGAGCT TCAGCGGCGA AGGCTGCCAG
    GTGAGGGTGG CCGCCCCGTC CTCCTCCCCG AGTGCCGAGG CCGAGTACTG GCGCATCCGA
    GCCATGGCTC AGCAGGTATT TATGTTGGAT ACCCAGTGCT CACCGAAGAC TCCAAACAAC
    TTCGATCATG CTCAGTCCTG TCAGCTCATT ATTGAACTGC CTCCTGATGA GAAGCCAAAT
    GGGCATACCA AGAAAAGTGT TTCTTTCAGG GAAATTGTGG TGAGTTTACT ATCCCATCAA
    GTATTACTCC AGAACTTATA TGATATCTTA TTAGAAGAGT TTGTCAAAGG CCCCTCTCCT
    GGGGAGGAGA AGATCCAAGG GCCAGAAACC AAGCTGGCTG GCTTCCTCAG ATACATCTCC
    ATGCAGAACC TGGCCGTCAT CTTTGACCTT CTGCTGGACT CCTACAGGAC GGCCAGAGAG
    TTTGACACCA GCCCAGGGCT GAAGTGCCTG CTGAAGAAAG TGTCTGGCAT AGGGGGCGCT
    GCCAACCTCT ACCGCCAGTC TGCCATGAGT TTCAACATTT ATTTCCACGC ACTGGTCTGT
    GCTGTTCTTA GCAACCAAGA AACCATCACT GCTGACCAAG TGAAGAAGGT CCTTTTTGAG
    GACGATGAGA GGAGCACAGA TTCGTCCCAG CAGTGTTCAT CAGAAGATGA AGACATTTTT
    GAGGAGACTG CCCAGGTGAG CCCCCCGAGA GGCAAAGAAA AGAGGCAGTG GAGGGCTCGG
    ATGCCCTCGC TCAGCGTCCA GCCCGTCAGC AACGCGGACT GGGTGTGGCT GGTCAAGAGG
    CTTCACAAGC TGTGCATGGA GCTGTGTAAC AACTACATCC AGATGCACCT GGACCTGGAA
    AACAGTATGG AGGAGGCCCC AATCTTCAAG AGTGACCCTT TCTTCATCCT TCCCTCCTTC
    CAGTCGGAGT CCTCCACCCC TTCCACCGGG GGCTTCTCAG GGAAAGACAC GCCTTCCGAG
    GATGACCGCA GTCAGACCCG GGATCATGCG GGCGAGCCTC TGAGCCTGAG GGGGACCAGC
    GGGGAGCTGC TGCTGCTGCC CCCGAGCCCC AAGGTGGAGA AGAAGGATCC CAGCCGCAAG
    AAGGAGTGGT GGGAGAACGC GGGCAACAAA ATCTATACCA TGGCAGCCGA CAAGACCATC
    TCAAAGCTGA TGACCGAGTA CAAGAGGAGG AAACAGCAGC ACAACCTGCC TGTCTTCCCC
    AAAGAGATCA AGGTGGAGAA GAAGGGAGAG GTGCTGGGCG CCAGGGGCCA AGGCTCCCCA
    CTGCTCCAGC GACCCCAGCA CCTGATGGAC CAGGGGCAGA TGCGGCACTC TTTCAGCGCC
    GGCCCTGAGC TGCTGCGCCA GGAGAAGAGG CCCCGCTCCG GCTCCACCGG CAGCTCCCGG
    AGTGTCTCCG CGAGGGACGC AGAAGCCCAG ATCCAGGCAT GGACCAACAT GGTGCTAACA
    GTCCTCAATC AGATTCAGAT CCTCCCAGAC CAGACCTTCA CAGCCCTCCA GCCAGCAGTG
    TTTCCGTGTA TCAGCCAGCT CACCTGTCAC GTGACTGACA TCAGAGTTCG CCAGGCTGTG
    AGAGAATGGC TCGGCAGAGT GGGCCGTGTC TATGACATCA TTATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.