ARFGEF3 cDNA ORF clone, Danio rerio(Zebrafish)

The following ARFGEF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARFGEF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa43857 XM_009307060.3
Latest version!
Danio rerio ARFGEF family member 3 (arfgef3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa43858 XM_009307061.3
Latest version!
Danio rerio ARFGEF family member 3 (arfgef3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
ODa43858 XM_009307061.2 Danio rerio ARFGEF family member 3 (arfgef3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa43857 XM_009307060.2 Danio rerio ARFGEF family member 3 (arfgef3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa43857
    Clone ID Related Accession (Same CDS sequence) XM_009307060.3 , XM_009307060.2
    Accession Version XM_009307060.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307060.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAGGAGA TTCTGCGGAG GCTTCAGAAG GAGGCGTCGG GCAGTAAATA CAGGGCGATC 
    CGGGACTCCT GCGGTGTGGC GTGCGAAACT CTTCAGGCTC AGAATGGATC AGTTAAAATA
    TCACCATCTA AACTCAGAGA GAAGTGTTTA TTCCCTCTTC AGTTGGCGCT GGAGTCTAAA
    AACACCAAAC TGGCACAGAC GGCCCTCACT GGGATGCAGA AGATCCTGTG TGAGGATTGT
    TTTCTCGCGG TGGAAGCAGA AGCTCCAGAG AAACAACTGT TGGGTCAAAT CCTGGAAGCT
    GTCAGAGTGA CGCCGAGCCT CCATGAAGAC CTGCAGGTGG AGGTTATGAA GGTCCTACTT
    TGCGTCACCT ACTCCTCGAC CTTCGAGATC ACTGGCCGCA ACATCTTGAA AATTGCTGAG
    GTATGTATAG AGACGTATAT ATCCAGTTGT CATCAGCGCA GTATCAACAC GGCGGTCAGA
    GCCACTCTCA GCCAGATTCT TGGAGACCTC ACTCTACAGC TACGCCATAG ACAAGAAAAC
    ACAGATGTTG ATGGTGAAGA ACAGTGTATT CCTGTGCACC AGCGCAAAGA TGTCTCTCCC
    AGTACTGAGG CGCTGTATGA AGATGTTGTC ATGGTCCTCA CTGTTTTCTG TGACAAACTG
    GAGTCCGTCT CAAATGAAAA CCAGCTGCTG CAGCTGTTGT ACCTGGAGTC TATTTTGTCC
    ATGCTCAGCA GCTGTCCTCC CACAATGCAC CTGCAGCGAA ACTTCACTGA CCTAGTCTGG
    AAGCAGTTAT GTCCCACCCT GGTGGTGATA ATGGGAAACC CAGCGAGTGA TAAGAGCATC
    ACCTCTGCTC ATGGCGCTCA GTGTCAGGAC TCAGTATCAG CCCCGGGGTC AGCGGTTCTG
    GATCAGGGCA GGGGTTCGGG ATGCTCCTCT AGTGCTCCGC TTATGATTGG GCCGGTGGTC
    CGGACCATCT GCTATGTGGC TGCAGAGTTG GTGCGTCTGG TAGGCTGTGT CGAGTCCATG
    AAACCTGTTT TGCAGTCTCT GTACCACCGT ATTCTGCTCT ACCCGCCACC CCAACACAGA
    GTGGAGGCCA TTAAGATTAT GAAAGAGATT GTGGGAAGCC CCCAGCGCTT GTTTGACCTC
    GCCGGTCCAT GCGTGATTGA ACACGAGACG AGAAAGAGGT CTTTCTCCAA AAGGAAATCA
    CATCTCGATC TTCTCAAACT CGTAATGGAT GGCATCACAG AAGCGTGTAT GAAGGGTGGG
    ATTGAGGCCT GTTACTCCTC CGTCTCCTGC GCCGGGGCCC TATTAGGTGC CTTGGATGAG
    CTCAGTCAGG GGCGTGGCCT GCAGCAGGAC CAGACCCGCA TGCTGCTGCG CCGATTGGAG
    GAGCTCAAGG AAGGGGCGGA GTCAATGCGT GAGTCTGTGG AAGTCAACGA AGCGGACTTC
    CGCTGGCAGC GGCGAGTGCT CTCATCCGAA AACGCTGCAT GGGAGGCGAG CATTACCGAA
    CGCAGCCCTG ACATTAGCAT TAGCGTCACC ACCGACACAG GCCAGACCAC TTTGGAAGGG
    GATGTAGGGC AGACTACGCT TGAAGGGGAT ATGGGGAGGA CCACTCCTGA AGATTGCGGG
    GACGAACCGC GACTACCTTC GCCTACCTGT GGATCTCGTC AGGAGTGTGT CGCACCTGAA
    ATCTCATCCT CTACGGCTCC TCCTGACGTA GTGCAACGCA GCCATGGCCT AGCCTATCCT
    GACATCACCA ACTTCTTATC GGTGGAGTCC CGGACGCGTT CGCATGGCTC CCGATACAGC
    GAGAGTAACT TCAGTGCCGA CGAGCAGGAG CTGTCCAGAA CAGAGTTTGA CTCCTGTGAT
    CAGTACTCCA TGGCTGCAGA AAAGGACTCC GGACGGTCTG ATGTATCCGA TATGGGCTCT
    GATAACTGCT CATTGGCTGA TGAGGAGCAG ACGCCGCGGG ACTGTCCCGG CCACCGTACA
    CTGCGGACTG CAGCTTTGTC TTTAAAGCTG CTGAAGAACC AGGAGGCGGA CCAACAGAGC
    GCAAGGCTCT TTGTGCAGTC TCTAGCCACA CTTCTGCCCC GTCTGCTGGG ACTGCACAGC
    ACCACAGATG TAGATGCGAC CCTGCAGAAC TTCTCCTCCA CCTTCTGCTC GGGACTACAA
    GCAGGTGGGG TTCATTCTCC AGGTTTCGAG GGCAATGAAA ACCTGAACTG TCAGGCTCTA
    ATGAACGCAG ACGGATTGTA CCTGGTCTCC TATTATGCTC TCCTGCTCAA CCTCAAGCTG
    TGCTGCTGTG ACTATTACCA CAGAAAGCCC ACGTCTGCTC TGGTGTCTCT GAAGGAGTTT
    GTGCGTCAGA TTCAGAGCAG TGGGGTTCTG GTGGTCTTGT CTCAGGCCTG GATAGAGGAG
    CTTTATAACC AGGTTTTGGA GAGAAACCTG CTGGGAGAAG CGGGATACTG GGGCTCGGCT
    GAGGAGCAGT CTTTACCCCT CATCACCATG CTCACTGATA TTGATGGACT GGGCAGCAGC
    GCTATTGGTG GTCAGCTGAT CAGAAAAGCC TCATCACAGT CACCCTTCAC CTGTGACAAA
    AACGGCAATG ACAGCCTGGT GGCAGGTGTG GTTTTTGCGC GTTACATCCT GACCGGCTGC
    TGGAAAAACC TGATCGACAC ACTGTCCACG CCGCTCACAG GGCGCATGGC TGGAAGCTCT
    CGCAGTCTGG CTTTCATTCT GGGGGCAGAG GGCGTTAAAG AGCAGACGCA GAGGGAAAGA
    GACACCATCT GCCTCAGTCT GGACGGTCTG CGCAAGGCTG CAGCGCTCAG CTGCGCTCTG
    GGGGTGGCAG CCAACTGTGC TTCAGCACTG GCGCAGATGG CAGCGGCTTC ATGTGTTCAG
    GAGGACAAAG AGGAGAAAGA ATTAGGAGAC ACTGGAGATG CCATTACTCA AGTGAAGCAG
    CGTGTGGAAC AGAAGCTGGA GCAGATGAGC CGTTCTCAGG GCGTGAGGCT TCACACTGCT
    CATGTCCTCT GCATGGACGC CATTCTCAAC GTGGGCCTGG AAATGGGCAG CCATAACCAC
    GACTGCTGGC CTCACGTCTT CAGGGTGATC GAGTATGTGA GTACACTGGA GCACACTCAC
    TTCAGTGACG GCGGCTCTCA GCCTCCTCTG GCCATCACGC AGGCGCAGCA GGCTGTAGCC
    GTGGATCTGG ATCTGGGTCT GGATCTGAGC GCAGAGGTCA GTCCGGAGCT GGAGCTGAGC
    CTCAGTCAGC CTGTCATCCA GCCGCTCTCC ATCCAGCAGC TCCTGAAGGA CAGCGCTCGC
    GGGAAAGCCT TGGATCTGCG GGGCGGGAGT CTGCTCACGG GAACCAGCGC AGCCAAAGCC
    GTCTGCACAC TTTCCACACA AGCTGACAGG CTGTTTGAGG ACGCCGTCAG TAAACTCAAC
    CTCGTGAGTC TTGTGGGATT CCTGCATCAG CTCAGAAAAG CTTCACAGTC TCAGCTGTTT
    GATTCCGTCA CAGAAACTGG AGACTATTCA CTGGCCATGC CAGGAGAGGC AAAGTCGACC
    CAGGACAAGC GCAGCGCCCT GCACCTCTTC CGGCTGGGAG AAGCCATGCT GCGCATCGTG
    AAGAACAAGA GCAGGCCGCT GCTGCACATG ATGAGAGCCT GGAGCGTTGT AGCACCACAC
    CTGGTGGAGG CGGCCTGTCA CAAGGAGCGC CACGTGTCCC AGAAGGCCGT GTCCTTCATC
    CATGATGTCC TGATGGAGGT TTTGAGCAGC TGGCCGGAGC TGCCGCATTT TCACTTCAAT
    GAGGCTCTTT TCAGACCGTT TGAGCACATC ATGCAGCTGG AGCTCTGCGA TGAAGACGTC
    CAGGATCAGG TGATCACTTC CATCGGAGAG CTGGTGGAGA TGTGTTCAGC GCAGATTCAG
    TCTGGATGGA GGCCGCTCTT CAGCGCTCTC AGAACCGTGC ATGGAAACAA GACGGATGTC
    AAGGATTATC TGATAGGAGA ATATTCTATG GGGAAGTCTC AGGCTCCAGT GTTTGATGTG
    TTTGAAGCCT TCATTAACAC AGACAACATT CAGGTGTTTG CTAACGCCGC CACCGACTAC
    ATCATGTGCC TTATGAAGTT TGTCAAAGGT TTAGGTGAAG TGGACTATAA GGAGATTGGC
    GATTGTGTTC ATGTCAGTGG ATACAGCTCT ACAGATCTGT GTCTGCCTGC GCTCGATTAC
    CTGAGGAAGT GCTCACAGCT GCTTGCCAAG ATCTATAAGA TGCCATCAAA GCCTGTGTTT
    CTGGGTGCGC GGCTGGCGAG TCTCCCAATG AGAGTGCAGG AGAGATCCGT GAGCAGCGAA
    GACGGCTTGG ACTGTGTGCT AGCCGAGTTT GATGACGATA CAGGTCTGAT TCAGGTGTGG
    ATCCTGCTGT TGGAGCAGCT GACAGCCGCT GTGTCCAACT GTCCCCGACA GCATCAGCCG
    CCGACCCTCG AGCTGCTGTT TGAGCTTCTG CGGGAGGTCA CCAAAATACC AGGGCCCGGT
    TTCGCCATCT TCTCTGTCAT CCAGCTGCTT CTTCCTGTGA TGTCACTGTG GCTCCAGCGT
    AGTCATGGCG ATCACTCATA CTGGGACATC GCAGCCGCAA ACTTCAAACA CGCCATCGGA
    CTGTCCTGTG AGCTGGTGGT GGAGCACATC CACAGCTTCA TACATTCAGA TATTGGCTAT
    GAGAGTCTGA TTAACCTGAT GCTGAAAGAT CTCTTCAAAC TGCTGGTTTC CTGTGTGGCA
    GAACCGGCTG AAACCATTTC CAGAGTCGGC TGCTCCTGTA TCAGGTATGT TCTGGTAACC
    GCCGGGCCTG TTTTCACTGA GGAGATGTGG CGTTTAGCAT GCTGTGCCCT GCAGGACGCT
    TTCTCAGCCA CTTTGGAGCC TGTCAAGAAC TTGCTGGCCT GTTTCCGGAG CGGTTCAGAC
    AGTTTCACTG GAGATGCGTG TGAAGTGAAA GTGGCAGCCC CGTCACACTC TCCCTCAGCA
    GAAGCAGAGA ACATGAGGAT TCGGGCCATG GCTCAACAGG TGTTCATGCT GGATACGCAG
    TGCTCTCCTA AAACTCCCAG CAGCAAGGAT GGGTTTGAGC ATGCTCAGTC CTGCGTGCTG
    ATCATTGAGC TTCCAGCTGA CCAGCACTCC AACGGCCAAG CTCAGAAGAG AAAAATCGGG
    ATCCCGTTCA GGACTCTGGT TGTGAGTTTA CTGTCCCATC AGGTGCTCCT GCAGAATCTG
    TATGACATCC TGTTAGAGGA GTTTGTCAAA GGTTCTGAGG GCAAAATCAC GGCTGTGTCG
    GAGCCAAAAC CTGCAGGATT TCTCCGCTAC ATCTCCATGC AGAACCTGGC CATCATCTTT
    GACCTCCTGC TGGACTCGTA TCGGACTGCC CGGGAATTTG ACACCCGTCC TGGCCTGAAG
    TACCTGCTCA TGAAAGTGTC CGGAGTGTGT GGAGCTGCTA ACTTATACCG CCAGTCTGCC
    ATGAGCTTCA ATATCTACTT TCAGGCTCTG CTTTGTGCAA TGCTGACAAA CCAGGAGAAC
    ATGAGCGTGG AGCAGGTGAA GAAGATCCTG AGTGAGGAAG AGGAGGGCGG ATCAGACTCC
    TCTCAGCAGT GCTCCTCCGA GGACGAGGAT ATATTTGAAG AAATGGCTCA AGTGAGTCCT
    CCACGTGCCC GAGAGAAACG TCAGTGGCAC ACGCGTATCC CATCTCTGAG CACGCAGCCC
    GCCAGCAGTG CCGACTGGGC CTGGCTGGTA AAACGTCTGC ACAAACTCTG CATGGACTTG
    TGCAACAACT ACATCCAGAT GCACCTGGAC CTGGAGAGTA CTTTGGGAGA GCAACCCACC
    CATCGTGCTG ACCCACTCTT TTTCTTGCCC CTTTTTAATT CTGAGACTTC CACGCCCTCA
    ACTGGTGGCC TGTCTGGCCA CGGGACTCCT TCAGAAGACA GTTTCCGCCT TAACCTGGAT
    GAGACGCCCT CAGAAGACGT TCATAGTCCC GGCGGGTCAA ACGCAAGCTT TCCCCAGCGG
    CGGGACGGGG GCCGCAAGAA GGAATGGTGG GAGAGTGCTG GAAACAAGCT GTACACCATA
    GCCACCGACA AGACCATTAC CAAACTGATG ATCGAGTACA AGAAACGCAA GCAGCAGCAG
    CAGCACAATC ACACCATGTT TGCCAAAGAT GTGAAGGCAG GAGAAAAGAG GGGCGAGCCT
    GTGACCCTAC GAGGCCCGGA CTCGCCCGGA CCGCAGAGGC CCCAACAGCT GGTTGAACAG
    GGGCCCATGA GGCATTCCTT TAGTGCGGGA CCCGAGCTGC TGCGGCAGGA GAAAAGACCA
    CGATCGGGAT CCACAGCCAG TTCACACAAC ATCTCTCTCA GAGATTCTGA GGCGCAGATA
    CAGGCCTGGA CAAACATGGT CCTGACCATC CTGAATCAGA TCCAGCTTCT GCCGGACCCC
    ACGTTCATGG CTCTGCAGCC GGCGGTGTTT CCCTGCATCA GTCAGCTGAC CTGTCATGTG
    ACCGAGCTGC GCGTGCGGCA GGCGGTGCGT GAGTGGCTGG GCCGAGTGGG ACGCGTTTAT
    GACATCATCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305335.1
    CDS330..6884
    Translation

    Target ORF information:

    RefSeq Version XM_009307060.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ARFGEF family member 3 (arfgef3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307060.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAGGAGA TTCTGCGGAG GCTTCAGAAG GAGGCGTCGG GCAGTAAATA CAGGGCGATC 
    CGGGACTCCT GCGGTGTGGC GTGCGAAACT CTTCAGGCTC AGAATGGATC AGTTAAAATA
    TCACCATCTA AACTCAGAGA GAAGTGTTTA TTCCCTCTTC AGTTGGCGCT GGAGTCTAAA
    AACACCAAAC TGGCACAGAC GGCCCTCACT GGGATGCAGA AGATCCTGTG TGAGGATTGT
    TTTCTCGCGG TGGAAGCAGA AGCTCCAGAG AAACAACTGT TGGGTCAAAT CCTGGAAGCT
    GTCAGAGTGA CGCCGAGCCT CCATGAAGAC CTGCAGGTGG AGGTTATGAA GGTCCTACTT
    TGCGTCACCT ACTCCTCGAC CTTCGAGATC ACTGGCCGCA ACATCTTGAA AATTGCTGAG
    GTATGTATAG AGACGTATAT ATCCAGTTGT CATCAGCGCA GTATCAACAC GGCGGTCAGA
    GCCACTCTCA GCCAGATTCT TGGAGACCTC ACTCTACAGC TACGCCATAG ACAAGAAAAC
    ACAGATGTTG ATGGTGAAGA ACAGTGTATT CCTGTGCACC AGCGCAAAGA TGTCTCTCCC
    AGTACTGAGG CGCTGTATGA AGATGTTGTC ATGGTCCTCA CTGTTTTCTG TGACAAACTG
    GAGTCCGTCT CAAATGAAAA CCAGCTGCTG CAGCTGTTGT ACCTGGAGTC TATTTTGTCC
    ATGCTCAGCA GCTGTCCTCC CACAATGCAC CTGCAGCGAA ACTTCACTGA CCTAGTCTGG
    AAGCAGTTAT GTCCCACCCT GGTGGTGATA ATGGGAAACC CAGCGAGTGA TAAGAGCATC
    ACCTCTGCTC ATGGCGCTCA GTGTCAGGAC TCAGTATCAG CCCCGGGGTC AGCGGTTCTG
    GATCAGGGCA GGGGTTCGGG ATGCTCCTCT AGTGCTCCGC TTATGATTGG GCCGGTGGTC
    CGGACCATCT GCTATGTGGC TGCAGAGTTG GTGCGTCTGG TAGGCTGTGT CGAGTCCATG
    AAACCTGTTT TGCAGTCTCT GTACCACCGT ATTCTGCTCT ACCCGCCACC CCAACACAGA
    GTGGAGGCCA TTAAGATTAT GAAAGAGATT GTGGGAAGCC CCCAGCGCTT GTTTGACCTC
    GCCGGTCCAT GCGTGATTGA ACACGAGACG AGAAAGAGGT CTTTCTCCAA AAGGAAATCA
    CATCTCGATC TTCTCAAACT CGTAATGGAT GGCATCACAG AAGCGTGTAT GAAGGGTGGG
    ATTGAGGCCT GTTACTCCTC CGTCTCCTGC GCCGGGGCCC TATTAGGTGC CTTGGATGAG
    CTCAGTCAGG GGCGTGGCCT GCAGCAGGAC CAGACCCGCA TGCTGCTGCG CCGATTGGAG
    GAGCTCAAGG AAGGGGCGGA GTCAATGCGT GAGTCTGTGG AAGTCAACGA AGCGGACTTC
    CGCTGGCAGC GGCGAGTGCT CTCATCCGAA AACGCTGCAT GGGAGGCGAG CATTACCGAA
    CGCAGCCCTG ACATTAGCAT TAGCGTCACC ACCGACACAG GCCAGACCAC TTTGGAAGGG
    GATGTAGGGC AGACTACGCT TGAAGGGGAT ATGGGGAGGA CCACTCCTGA AGATTGCGGG
    GACGAACCGC GACTACCTTC GCCTACCTGT GGATCTCGTC AGGAGTGTGT CGCACCTGAA
    ATCTCATCCT CTACGGCTCC TCCTGACGTA GTGCAACGCA GCCATGGCCT AGCCTATCCT
    GACATCACCA ACTTCTTATC GGTGGAGTCC CGGACGCGTT CGCATGGCTC CCGATACAGC
    GAGAGTAACT TCAGTGCCGA CGAGCAGGAG CTGTCCAGAA CAGAGTTTGA CTCCTGTGAT
    CAGTACTCCA TGGCTGCAGA AAAGGACTCC GGACGGTCTG ATGTATCCGA TATGGGCTCT
    GATAACTGCT CATTGGCTGA TGAGGAGCAG ACGCCGCGGG ACTGTCCCGG CCACCGTACA
    CTGCGGACTG CAGCTTTGTC TTTAAAGCTG CTGAAGAACC AGGAGGCGGA CCAACAGAGC
    GCAAGGCTCT TTGTGCAGTC TCTAGCCACA CTTCTGCCCC GTCTGCTGGG ACTGCACAGC
    ACCACAGATG TAGATGCGAC CCTGCAGAAC TTCTCCTCCA CCTTCTGCTC GGGACTACAA
    GCAGGTGGGG TTCATTCTCC AGGTTTCGAG GGCAATGAAA ACCTGAACTG TCAGGCTCTA
    ATGAACGCAG ACGGATTGTA CCTGGTCTCC TATTATGCTC TCCTGCTCAA CCTCAAGCTG
    TGCTGCTGTG ACTATTACCA CAGAAAGCCC ACGTCTGCTC TGGTGTCTCT GAAGGAGTTT
    GTGCGTCAGA TTCAGAGCAG TGGGGTTCTG GTGGTCTTGT CTCAGGCCTG GATAGAGGAG
    CTTTATAACC AGGTTTTGGA GAGAAACCTG CTGGGAGAAG CGGGATACTG GGGCTCGGCT
    GAGGAGCAGT CTTTACCCCT CATCACCATG CTCACTGATA TTGATGGACT GGGCAGCAGC
    GCTATTGGTG GTCAGCTGAT CAGAAAAGCC TCATCACAGT CACCCTTCAC CTGTGACAAA
    AACGGCAATG ACAGCCTGGT GGCAGGTGTG GTTTTTGCGC GTTACATCCT GACCGGCTGC
    TGGAAAAACC TGATCGACAC ACTGTCCACG CCGCTCACAG GGCGCATGGC TGGAAGCTCT
    CGCAGTCTGG CTTTCATTCT GGGGGCAGAG GGCGTTAAAG AGCAGACGCA GAGGGAAAGA
    GACACCATCT GCCTCAGTCT GGACGGTCTG CGCAAGGCTG CAGCGCTCAG CTGCGCTCTG
    GGGGTGGCAG CCAACTGTGC TTCAGCACTG GCGCAGATGG CAGCGGCTTC ATGTGTTCAG
    GAGGACAAAG AGGAGAAAGA ATTAGGAGAC ACTGGAGATG CCATTACTCA AGTGAAGCAG
    CGTGTGGAAC AGAAGCTGGA GCAGATGAGC CGTTCTCAGG GCGTGAGGCT TCACACTGCT
    CATGTCCTCT GCATGGACGC CATTCTCAAC GTGGGCCTGG AAATGGGCAG CCATAACCAC
    GACTGCTGGC CTCACGTCTT CAGGGTGATC GAGTATGTGA GTACACTGGA GCACACTCAC
    TTCAGTGACG GCGGCTCTCA GCCTCCTCTG GCCATCACGC AGGCGCAGCA GGCTGTAGCC
    GTGGATCTGG ATCTGGGTCT GGATCTGAGC GCAGAGGTCA GTCCGGAGCT GGAGCTGAGC
    CTCAGTCAGC CTGTCATCCA GCCGCTCTCC ATCCAGCAGC TCCTGAAGGA CAGCGCTCGC
    GGGAAAGCCT TGGATCTGCG GGGCGGGAGT CTGCTCACGG GAACCAGCGC AGCCAAAGCC
    GTCTGCACAC TTTCCACACA AGCTGACAGG CTGTTTGAGG ACGCCGTCAG TAAACTCAAC
    CTCGTGAGTC TTGTGGGATT CCTGCATCAG CTCAGAAAAG CTTCACAGTC TCAGCTGTTT
    GATTCCGTCA CAGAAACTGG AGACTATTCA CTGGCCATGC CAGGAGAGGC AAAGTCGACC
    CAGGACAAGC GCAGCGCCCT GCACCTCTTC CGGCTGGGAG AAGCCATGCT GCGCATCGTG
    AAGAACAAGA GCAGGCCGCT GCTGCACATG ATGAGAGCCT GGAGCGTTGT AGCACCACAC
    CTGGTGGAGG CGGCCTGTCA CAAGGAGCGC CACGTGTCCC AGAAGGCCGT GTCCTTCATC
    CATGATGTCC TGATGGAGGT TTTGAGCAGC TGGCCGGAGC TGCCGCATTT TCACTTCAAT
    GAGGCTCTTT TCAGACCGTT TGAGCACATC ATGCAGCTGG AGCTCTGCGA TGAAGACGTC
    CAGGATCAGG TGATCACTTC CATCGGAGAG CTGGTGGAGA TGTGTTCAGC GCAGATTCAG
    TCTGGATGGA GGCCGCTCTT CAGCGCTCTC AGAACCGTGC ATGGAAACAA GACGGATGTC
    AAGGATTATC TGATAGGAGA ATATTCTATG GGGAAGTCTC AGGCTCCAGT GTTTGATGTG
    TTTGAAGCCT TCATTAACAC AGACAACATT CAGGTGTTTG CTAACGCCGC CACCGACTAC
    ATCATGTGCC TTATGAAGTT TGTCAAAGGT TTAGGTGAAG TGGACTATAA GGAGATTGGC
    GATTGTGTTC ATGTCAGTGG ATACAGCTCT ACAGATCTGT GTCTGCCTGC GCTCGATTAC
    CTGAGGAAGT GCTCACAGCT GCTTGCCAAG ATCTATAAGA TGCCATCAAA GCCTGTGTTT
    CTGGGTGCGC GGCTGGCGAG TCTCCCAATG AGAGTGCAGG AGAGATCCGT GAGCAGCGAA
    GACGGCTTGG ACTGTGTGCT AGCCGAGTTT GATGACGATA CAGGTCTGAT TCAGGTGTGG
    ATCCTGCTGT TGGAGCAGCT GACAGCCGCT GTGTCCAACT GTCCCCGACA GCATCAGCCG
    CCGACCCTCG AGCTGCTGTT TGAGCTTCTG CGGGAGGTCA CCAAAATACC AGGGCCCGGT
    TTCGCCATCT TCTCTGTCAT CCAGCTGCTT CTTCCTGTGA TGTCACTGTG GCTCCAGCGT
    AGTCATGGCG ATCACTCATA CTGGGACATC GCAGCCGCAA ACTTCAAACA CGCCATCGGA
    CTGTCCTGTG AGCTGGTGGT GGAGCACATC CACAGCTTCA TACATTCAGA TATTGGCTAT
    GAGAGTCTGA TTAACCTGAT GCTGAAAGAT CTCTTCAAAC TGCTGGTTTC CTGTGTGGCA
    GAACCGGCTG AAACCATTTC CAGAGTCGGC TGCTCCTGTA TCAGGTATGT TCTGGTAACC
    GCCGGGCCTG TTTTCACTGA GGAGATGTGG CGTTTAGCAT GCTGTGCCCT GCAGGACGCT
    TTCTCAGCCA CTTTGGAGCC TGTCAAGAAC TTGCTGGCCT GTTTCCGGAG CGGTTCAGAC
    AGTTTCACTG GAGATGCGTG TGAAGTGAAA GTGGCAGCCC CGTCACACTC TCCCTCAGCA
    GAAGCAGAGA ACATGAGGAT TCGGGCCATG GCTCAACAGG TGTTCATGCT GGATACGCAG
    TGCTCTCCTA AAACTCCCAG CAGCAAGGAT GGGTTTGAGC ATGCTCAGTC CTGCGTGCTG
    ATCATTGAGC TTCCAGCTGA CCAGCACTCC AACGGCCAAG CTCAGAAGAG AAAAATCGGG
    ATCCCGTTCA GGACTCTGGT TGTGAGTTTA CTGTCCCATC AGGTGCTCCT GCAGAATCTG
    TATGACATCC TGTTAGAGGA GTTTGTCAAA GGTTCTGAGG GCAAAATCAC GGCTGTGTCG
    GAGCCAAAAC CTGCAGGATT TCTCCGCTAC ATCTCCATGC AGAACCTGGC CATCATCTTT
    GACCTCCTGC TGGACTCGTA TCGGACTGCC CGGGAATTTG ACACCCGTCC TGGCCTGAAG
    TACCTGCTCA TGAAAGTGTC CGGAGTGTGT GGAGCTGCTA ACTTATACCG CCAGTCTGCC
    ATGAGCTTCA ATATCTACTT TCAGGCTCTG CTTTGTGCAA TGCTGACAAA CCAGGAGAAC
    ATGAGCGTGG AGCAGGTGAA GAAGATCCTG AGTGAGGAAG AGGAGGGCGG ATCAGACTCC
    TCTCAGCAGT GCTCCTCCGA GGACGAGGAT ATATTTGAAG AAATGGCTCA AGTGAGTCCT
    CCACGTGCCC GAGAGAAACG TCAGTGGCAC ACGCGTATCC CATCTCTGAG CACGCAGCCC
    GCCAGCAGTG CCGACTGGGC CTGGCTGGTA AAACGTCTGC ACAAACTCTG CATGGACTTG
    TGCAACAACT ACATCCAGAT GCACCTGGAC CTGGAGAGTA CTTTGGGAGA GCAACCCACC
    CATCGTGCTG ACCCACTCTT TTTCTTGCCC CTTTTTAATT CTGAGACTTC CACGCCCTCA
    ACTGGTGGCC TGTCTGGCCA CGGGACTCCT TCAGAAGACA GTTTCCGCCT TAACCTGGAT
    GAGACGCCCT CAGAAGACGT TCATAGTCCC GGCGGGTCAA ACGCAAGCTT TCCCCAGCGG
    CGGGACGGGG GCCGCAAGAA GGAATGGTGG GAGAGTGCTG GAAACAAGCT GTACACCATA
    GCCACCGACA AGACCATTAC CAAACTGATG ATCGAGTACA AGAAACGCAA GCAGCAGCAG
    CAGCACAATC ACACCATGTT TGCCAAAGAT GTGAAGGCAG GAGAAAAGAG GGGCGAGCCT
    GTGACCCTAC GAGGCCCGGA CTCGCCCGGA CCGCAGAGGC CCCAACAGCT GGTTGAACAG
    GGGCCCATGA GGCATTCCTT TAGTGCGGGA CCCGAGCTGC TGCGGCAGGA GAAAAGACCA
    CGATCGGGAT CCACAGCCAG TTCACACAAC ATCTCTCTCA GAGATTCTGA GGCGCAGATA
    CAGGCCTGGA CAAACATGGT CCTGACCATC CTGAATCAGA TCCAGCTTCT GCCGGACCCC
    ACGTTCATGG CTCTGCAGCC GGCGGTGTTT CCCTGCATCA GTCAGCTGAC CTGTCATGTG
    ACCGAGCTGC GCGTGCGGCA GGCGGTGCGT GAGTGGCTGG GCCGAGTGGG ACGCGTTTAT
    GACATCATCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43857
    Clone ID Related Accession (Same CDS sequence) XM_009307060.3 , XM_009307060.2
    Accession Version XM_009307060.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307060.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAGGAGA TTCTGCGGAG GCTTCAGAAG GAGGCGTCGG GCAGTAAATA CAGGGCGATC 
    CGGGACTCCT GCGGTGTGGC GTGCGAAACT CTTCAGGCTC AGAATGGATC AGTTAAAATA
    TCACCATCTA AACTCAGAGA GAAGTGTTTA TTCCCTCTTC AGTTGGCGCT GGAGTCTAAA
    AACACCAAAC TGGCACAGAC GGCCCTCACT GGGATGCAGA AGATCCTGTG TGAGGATTGT
    TTTCTCGCGG TGGAAGCAGA AGCTCCAGAG AAACAACTGT TGGGTCAAAT CCTGGAAGCT
    GTCAGAGTGA CGCCGAGCCT CCATGAAGAC CTGCAGGTGG AGGTTATGAA GGTCCTACTT
    TGCGTCACCT ACTCCTCGAC CTTCGAGATC ACTGGCCGCA ACATCTTGAA AATTGCTGAG
    GTATGTATAG AGACGTATAT ATCCAGTTGT CATCAGCGCA GTATCAACAC GGCGGTCAGA
    GCCACTCTCA GCCAGATTCT TGGAGACCTC ACTCTACAGC TACGCCATAG ACAAGAAAAC
    ACAGATGTTG ATGGTGAAGA ACAGTGTATT CCTGTGCACC AGCGCAAAGA TGTCTCTCCC
    AGTACTGAGG CGCTGTATGA AGATGTTGTC ATGGTCCTCA CTGTTTTCTG TGACAAACTG
    GAGTCCGTCT CAAATGAAAA CCAGCTGCTG CAGCTGTTGT ACCTGGAGTC TATTTTGTCC
    ATGCTCAGCA GCTGTCCTCC CACAATGCAC CTGCAGCGAA ACTTCACTGA CCTAGTCTGG
    AAGCAGTTAT GTCCCACCCT GGTGGTGATA ATGGGAAACC CAGCGAGTGA TAAGAGCATC
    ACCTCTGCTC ATGGCGCTCA GTGTCAGGAC TCAGTATCAG CCCCGGGGTC AGCGGTTCTG
    GATCAGGGCA GGGGTTCGGG ATGCTCCTCT AGTGCTCCGC TTATGATTGG GCCGGTGGTC
    CGGACCATCT GCTATGTGGC TGCAGAGTTG GTGCGTCTGG TAGGCTGTGT CGAGTCCATG
    AAACCTGTTT TGCAGTCTCT GTACCACCGT ATTCTGCTCT ACCCGCCACC CCAACACAGA
    GTGGAGGCCA TTAAGATTAT GAAAGAGATT GTGGGAAGCC CCCAGCGCTT GTTTGACCTC
    GCCGGTCCAT GCGTGATTGA ACACGAGACG AGAAAGAGGT CTTTCTCCAA AAGGAAATCA
    CATCTCGATC TTCTCAAACT CGTAATGGAT GGCATCACAG AAGCGTGTAT GAAGGGTGGG
    ATTGAGGCCT GTTACTCCTC CGTCTCCTGC GCCGGGGCCC TATTAGGTGC CTTGGATGAG
    CTCAGTCAGG GGCGTGGCCT GCAGCAGGAC CAGACCCGCA TGCTGCTGCG CCGATTGGAG
    GAGCTCAAGG AAGGGGCGGA GTCAATGCGT GAGTCTGTGG AAGTCAACGA AGCGGACTTC
    CGCTGGCAGC GGCGAGTGCT CTCATCCGAA AACGCTGCAT GGGAGGCGAG CATTACCGAA
    CGCAGCCCTG ACATTAGCAT TAGCGTCACC ACCGACACAG GCCAGACCAC TTTGGAAGGG
    GATGTAGGGC AGACTACGCT TGAAGGGGAT ATGGGGAGGA CCACTCCTGA AGATTGCGGG
    GACGAACCGC GACTACCTTC GCCTACCTGT GGATCTCGTC AGGAGTGTGT CGCACCTGAA
    ATCTCATCCT CTACGGCTCC TCCTGACGTA GTGCAACGCA GCCATGGCCT AGCCTATCCT
    GACATCACCA ACTTCTTATC GGTGGAGTCC CGGACGCGTT CGCATGGCTC CCGATACAGC
    GAGAGTAACT TCAGTGCCGA CGAGCAGGAG CTGTCCAGAA CAGAGTTTGA CTCCTGTGAT
    CAGTACTCCA TGGCTGCAGA AAAGGACTCC GGACGGTCTG ATGTATCCGA TATGGGCTCT
    GATAACTGCT CATTGGCTGA TGAGGAGCAG ACGCCGCGGG ACTGTCCCGG CCACCGTACA
    CTGCGGACTG CAGCTTTGTC TTTAAAGCTG CTGAAGAACC AGGAGGCGGA CCAACAGAGC
    GCAAGGCTCT TTGTGCAGTC TCTAGCCACA CTTCTGCCCC GTCTGCTGGG ACTGCACAGC
    ACCACAGATG TAGATGCGAC CCTGCAGAAC TTCTCCTCCA CCTTCTGCTC GGGACTACAA
    GCAGGTGGGG TTCATTCTCC AGGTTTCGAG GGCAATGAAA ACCTGAACTG TCAGGCTCTA
    ATGAACGCAG ACGGATTGTA CCTGGTCTCC TATTATGCTC TCCTGCTCAA CCTCAAGCTG
    TGCTGCTGTG ACTATTACCA CAGAAAGCCC ACGTCTGCTC TGGTGTCTCT GAAGGAGTTT
    GTGCGTCAGA TTCAGAGCAG TGGGGTTCTG GTGGTCTTGT CTCAGGCCTG GATAGAGGAG
    CTTTATAACC AGGTTTTGGA GAGAAACCTG CTGGGAGAAG CGGGATACTG GGGCTCGGCT
    GAGGAGCAGT CTTTACCCCT CATCACCATG CTCACTGATA TTGATGGACT GGGCAGCAGC
    GCTATTGGTG GTCAGCTGAT CAGAAAAGCC TCATCACAGT CACCCTTCAC CTGTGACAAA
    AACGGCAATG ACAGCCTGGT GGCAGGTGTG GTTTTTGCGC GTTACATCCT GACCGGCTGC
    TGGAAAAACC TGATCGACAC ACTGTCCACG CCGCTCACAG GGCGCATGGC TGGAAGCTCT
    CGCAGTCTGG CTTTCATTCT GGGGGCAGAG GGCGTTAAAG AGCAGACGCA GAGGGAAAGA
    GACACCATCT GCCTCAGTCT GGACGGTCTG CGCAAGGCTG CAGCGCTCAG CTGCGCTCTG
    GGGGTGGCAG CCAACTGTGC TTCAGCACTG GCGCAGATGG CAGCGGCTTC ATGTGTTCAG
    GAGGACAAAG AGGAGAAAGA ATTAGGAGAC ACTGGAGATG CCATTACTCA AGTGAAGCAG
    CGTGTGGAAC AGAAGCTGGA GCAGATGAGC CGTTCTCAGG GCGTGAGGCT TCACACTGCT
    CATGTCCTCT GCATGGACGC CATTCTCAAC GTGGGCCTGG AAATGGGCAG CCATAACCAC
    GACTGCTGGC CTCACGTCTT CAGGGTGATC GAGTATGTGA GTACACTGGA GCACACTCAC
    TTCAGTGACG GCGGCTCTCA GCCTCCTCTG GCCATCACGC AGGCGCAGCA GGCTGTAGCC
    GTGGATCTGG ATCTGGGTCT GGATCTGAGC GCAGAGGTCA GTCCGGAGCT GGAGCTGAGC
    CTCAGTCAGC CTGTCATCCA GCCGCTCTCC ATCCAGCAGC TCCTGAAGGA CAGCGCTCGC
    GGGAAAGCCT TGGATCTGCG GGGCGGGAGT CTGCTCACGG GAACCAGCGC AGCCAAAGCC
    GTCTGCACAC TTTCCACACA AGCTGACAGG CTGTTTGAGG ACGCCGTCAG TAAACTCAAC
    CTCGTGAGTC TTGTGGGATT CCTGCATCAG CTCAGAAAAG CTTCACAGTC TCAGCTGTTT
    GATTCCGTCA CAGAAACTGG AGACTATTCA CTGGCCATGC CAGGAGAGGC AAAGTCGACC
    CAGGACAAGC GCAGCGCCCT GCACCTCTTC CGGCTGGGAG AAGCCATGCT GCGCATCGTG
    AAGAACAAGA GCAGGCCGCT GCTGCACATG ATGAGAGCCT GGAGCGTTGT AGCACCACAC
    CTGGTGGAGG CGGCCTGTCA CAAGGAGCGC CACGTGTCCC AGAAGGCCGT GTCCTTCATC
    CATGATGTCC TGATGGAGGT TTTGAGCAGC TGGCCGGAGC TGCCGCATTT TCACTTCAAT
    GAGGCTCTTT TCAGACCGTT TGAGCACATC ATGCAGCTGG AGCTCTGCGA TGAAGACGTC
    CAGGATCAGG TGATCACTTC CATCGGAGAG CTGGTGGAGA TGTGTTCAGC GCAGATTCAG
    TCTGGATGGA GGCCGCTCTT CAGCGCTCTC AGAACCGTGC ATGGAAACAA GACGGATGTC
    AAGGATTATC TGATAGGAGA ATATTCTATG GGGAAGTCTC AGGCTCCAGT GTTTGATGTG
    TTTGAAGCCT TCATTAACAC AGACAACATT CAGGTGTTTG CTAACGCCGC CACCGACTAC
    ATCATGTGCC TTATGAAGTT TGTCAAAGGT TTAGGTGAAG TGGACTATAA GGAGATTGGC
    GATTGTGTTC ATGTCAGTGG ATACAGCTCT ACAGATCTGT GTCTGCCTGC GCTCGATTAC
    CTGAGGAAGT GCTCACAGCT GCTTGCCAAG ATCTATAAGA TGCCATCAAA GCCTGTGTTT
    CTGGGTGCGC GGCTGGCGAG TCTCCCAATG AGAGTGCAGG AGAGATCCGT GAGCAGCGAA
    GACGGCTTGG ACTGTGTGCT AGCCGAGTTT GATGACGATA CAGGTCTGAT TCAGGTGTGG
    ATCCTGCTGT TGGAGCAGCT GACAGCCGCT GTGTCCAACT GTCCCCGACA GCATCAGCCG
    CCGACCCTCG AGCTGCTGTT TGAGCTTCTG CGGGAGGTCA CCAAAATACC AGGGCCCGGT
    TTCGCCATCT TCTCTGTCAT CCAGCTGCTT CTTCCTGTGA TGTCACTGTG GCTCCAGCGT
    AGTCATGGCG ATCACTCATA CTGGGACATC GCAGCCGCAA ACTTCAAACA CGCCATCGGA
    CTGTCCTGTG AGCTGGTGGT GGAGCACATC CACAGCTTCA TACATTCAGA TATTGGCTAT
    GAGAGTCTGA TTAACCTGAT GCTGAAAGAT CTCTTCAAAC TGCTGGTTTC CTGTGTGGCA
    GAACCGGCTG AAACCATTTC CAGAGTCGGC TGCTCCTGTA TCAGGTATGT TCTGGTAACC
    GCCGGGCCTG TTTTCACTGA GGAGATGTGG CGTTTAGCAT GCTGTGCCCT GCAGGACGCT
    TTCTCAGCCA CTTTGGAGCC TGTCAAGAAC TTGCTGGCCT GTTTCCGGAG CGGTTCAGAC
    AGTTTCACTG GAGATGCGTG TGAAGTGAAA GTGGCAGCCC CGTCACACTC TCCCTCAGCA
    GAAGCAGAGA ACATGAGGAT TCGGGCCATG GCTCAACAGG TGTTCATGCT GGATACGCAG
    TGCTCTCCTA AAACTCCCAG CAGCAAGGAT GGGTTTGAGC ATGCTCAGTC CTGCGTGCTG
    ATCATTGAGC TTCCAGCTGA CCAGCACTCC AACGGCCAAG CTCAGAAGAG AAAAATCGGG
    ATCCCGTTCA GGACTCTGGT TGTGAGTTTA CTGTCCCATC AGGTGCTCCT GCAGAATCTG
    TATGACATCC TGTTAGAGGA GTTTGTCAAA GGTTCTGAGG GCAAAATCAC GGCTGTGTCG
    GAGCCAAAAC CTGCAGGATT TCTCCGCTAC ATCTCCATGC AGAACCTGGC CATCATCTTT
    GACCTCCTGC TGGACTCGTA TCGGACTGCC CGGGAATTTG ACACCCGTCC TGGCCTGAAG
    TACCTGCTCA TGAAAGTGTC CGGAGTGTGT GGAGCTGCTA ACTTATACCG CCAGTCTGCC
    ATGAGCTTCA ATATCTACTT TCAGGCTCTG CTTTGTGCAA TGCTGACAAA CCAGGAGAAC
    ATGAGCGTGG AGCAGGTGAA GAAGATCCTG AGTGAGGAAG AGGAGGGCGG ATCAGACTCC
    TCTCAGCAGT GCTCCTCCGA GGACGAGGAT ATATTTGAAG AAATGGCTCA AGTGAGTCCT
    CCACGTGCCC GAGAGAAACG TCAGTGGCAC ACGCGTATCC CATCTCTGAG CACGCAGCCC
    GCCAGCAGTG CCGACTGGGC CTGGCTGGTA AAACGTCTGC ACAAACTCTG CATGGACTTG
    TGCAACAACT ACATCCAGAT GCACCTGGAC CTGGAGAGTA CTTTGGGAGA GCAACCCACC
    CATCGTGCTG ACCCACTCTT TTTCTTGCCC CTTTTTAATT CTGAGACTTC CACGCCCTCA
    ACTGGTGGCC TGTCTGGCCA CGGGACTCCT TCAGAAGACA GTTTCCGCCT TAACCTGGAT
    GAGACGCCCT CAGAAGACGT TCATAGTCCC GGCGGGTCAA ACGCAAGCTT TCCCCAGCGG
    CGGGACGGGG GCCGCAAGAA GGAATGGTGG GAGAGTGCTG GAAACAAGCT GTACACCATA
    GCCACCGACA AGACCATTAC CAAACTGATG ATCGAGTACA AGAAACGCAA GCAGCAGCAG
    CAGCACAATC ACACCATGTT TGCCAAAGAT GTGAAGGCAG GAGAAAAGAG GGGCGAGCCT
    GTGACCCTAC GAGGCCCGGA CTCGCCCGGA CCGCAGAGGC CCCAACAGCT GGTTGAACAG
    GGGCCCATGA GGCATTCCTT TAGTGCGGGA CCCGAGCTGC TGCGGCAGGA GAAAAGACCA
    CGATCGGGAT CCACAGCCAG TTCACACAAC ATCTCTCTCA GAGATTCTGA GGCGCAGATA
    CAGGCCTGGA CAAACATGGT CCTGACCATC CTGAATCAGA TCCAGCTTCT GCCGGACCCC
    ACGTTCATGG CTCTGCAGCC GGCGGTGTTT CCCTGCATCA GTCAGCTGAC CTGTCATGTG
    ACCGAGCTGC GCGTGCGGCA GGCGGTGCGT GAGTGGCTGG GCCGAGTGGG ACGCGTTTAT
    GACATCATCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305335.1
    CDS281..6835
    Translation

    Target ORF information:

    RefSeq Version XM_009307060.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ARFGEF family member 3 (arfgef3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307060.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAGGAGA TTCTGCGGAG GCTTCAGAAG GAGGCGTCGG GCAGTAAATA CAGGGCGATC 
    CGGGACTCCT GCGGTGTGGC GTGCGAAACT CTTCAGGCTC AGAATGGATC AGTTAAAATA
    TCACCATCTA AACTCAGAGA GAAGTGTTTA TTCCCTCTTC AGTTGGCGCT GGAGTCTAAA
    AACACCAAAC TGGCACAGAC GGCCCTCACT GGGATGCAGA AGATCCTGTG TGAGGATTGT
    TTTCTCGCGG TGGAAGCAGA AGCTCCAGAG AAACAACTGT TGGGTCAAAT CCTGGAAGCT
    GTCAGAGTGA CGCCGAGCCT CCATGAAGAC CTGCAGGTGG AGGTTATGAA GGTCCTACTT
    TGCGTCACCT ACTCCTCGAC CTTCGAGATC ACTGGCCGCA ACATCTTGAA AATTGCTGAG
    GTATGTATAG AGACGTATAT ATCCAGTTGT CATCAGCGCA GTATCAACAC GGCGGTCAGA
    GCCACTCTCA GCCAGATTCT TGGAGACCTC ACTCTACAGC TACGCCATAG ACAAGAAAAC
    ACAGATGTTG ATGGTGAAGA ACAGTGTATT CCTGTGCACC AGCGCAAAGA TGTCTCTCCC
    AGTACTGAGG CGCTGTATGA AGATGTTGTC ATGGTCCTCA CTGTTTTCTG TGACAAACTG
    GAGTCCGTCT CAAATGAAAA CCAGCTGCTG CAGCTGTTGT ACCTGGAGTC TATTTTGTCC
    ATGCTCAGCA GCTGTCCTCC CACAATGCAC CTGCAGCGAA ACTTCACTGA CCTAGTCTGG
    AAGCAGTTAT GTCCCACCCT GGTGGTGATA ATGGGAAACC CAGCGAGTGA TAAGAGCATC
    ACCTCTGCTC ATGGCGCTCA GTGTCAGGAC TCAGTATCAG CCCCGGGGTC AGCGGTTCTG
    GATCAGGGCA GGGGTTCGGG ATGCTCCTCT AGTGCTCCGC TTATGATTGG GCCGGTGGTC
    CGGACCATCT GCTATGTGGC TGCAGAGTTG GTGCGTCTGG TAGGCTGTGT CGAGTCCATG
    AAACCTGTTT TGCAGTCTCT GTACCACCGT ATTCTGCTCT ACCCGCCACC CCAACACAGA
    GTGGAGGCCA TTAAGATTAT GAAAGAGATT GTGGGAAGCC CCCAGCGCTT GTTTGACCTC
    GCCGGTCCAT GCGTGATTGA ACACGAGACG AGAAAGAGGT CTTTCTCCAA AAGGAAATCA
    CATCTCGATC TTCTCAAACT CGTAATGGAT GGCATCACAG AAGCGTGTAT GAAGGGTGGG
    ATTGAGGCCT GTTACTCCTC CGTCTCCTGC GCCGGGGCCC TATTAGGTGC CTTGGATGAG
    CTCAGTCAGG GGCGTGGCCT GCAGCAGGAC CAGACCCGCA TGCTGCTGCG CCGATTGGAG
    GAGCTCAAGG AAGGGGCGGA GTCAATGCGT GAGTCTGTGG AAGTCAACGA AGCGGACTTC
    CGCTGGCAGC GGCGAGTGCT CTCATCCGAA AACGCTGCAT GGGAGGCGAG CATTACCGAA
    CGCAGCCCTG ACATTAGCAT TAGCGTCACC ACCGACACAG GCCAGACCAC TTTGGAAGGG
    GATGTAGGGC AGACTACGCT TGAAGGGGAT ATGGGGAGGA CCACTCCTGA AGATTGCGGG
    GACGAACCGC GACTACCTTC GCCTACCTGT GGATCTCGTC AGGAGTGTGT CGCACCTGAA
    ATCTCATCCT CTACGGCTCC TCCTGACGTA GTGCAACGCA GCCATGGCCT AGCCTATCCT
    GACATCACCA ACTTCTTATC GGTGGAGTCC CGGACGCGTT CGCATGGCTC CCGATACAGC
    GAGAGTAACT TCAGTGCCGA CGAGCAGGAG CTGTCCAGAA CAGAGTTTGA CTCCTGTGAT
    CAGTACTCCA TGGCTGCAGA AAAGGACTCC GGACGGTCTG ATGTATCCGA TATGGGCTCT
    GATAACTGCT CATTGGCTGA TGAGGAGCAG ACGCCGCGGG ACTGTCCCGG CCACCGTACA
    CTGCGGACTG CAGCTTTGTC TTTAAAGCTG CTGAAGAACC AGGAGGCGGA CCAACAGAGC
    GCAAGGCTCT TTGTGCAGTC TCTAGCCACA CTTCTGCCCC GTCTGCTGGG ACTGCACAGC
    ACCACAGATG TAGATGCGAC CCTGCAGAAC TTCTCCTCCA CCTTCTGCTC GGGACTACAA
    GCAGGTGGGG TTCATTCTCC AGGTTTCGAG GGCAATGAAA ACCTGAACTG TCAGGCTCTA
    ATGAACGCAG ACGGATTGTA CCTGGTCTCC TATTATGCTC TCCTGCTCAA CCTCAAGCTG
    TGCTGCTGTG ACTATTACCA CAGAAAGCCC ACGTCTGCTC TGGTGTCTCT GAAGGAGTTT
    GTGCGTCAGA TTCAGAGCAG TGGGGTTCTG GTGGTCTTGT CTCAGGCCTG GATAGAGGAG
    CTTTATAACC AGGTTTTGGA GAGAAACCTG CTGGGAGAAG CGGGATACTG GGGCTCGGCT
    GAGGAGCAGT CTTTACCCCT CATCACCATG CTCACTGATA TTGATGGACT GGGCAGCAGC
    GCTATTGGTG GTCAGCTGAT CAGAAAAGCC TCATCACAGT CACCCTTCAC CTGTGACAAA
    AACGGCAATG ACAGCCTGGT GGCAGGTGTG GTTTTTGCGC GTTACATCCT GACCGGCTGC
    TGGAAAAACC TGATCGACAC ACTGTCCACG CCGCTCACAG GGCGCATGGC TGGAAGCTCT
    CGCAGTCTGG CTTTCATTCT GGGGGCAGAG GGCGTTAAAG AGCAGACGCA GAGGGAAAGA
    GACACCATCT GCCTCAGTCT GGACGGTCTG CGCAAGGCTG CAGCGCTCAG CTGCGCTCTG
    GGGGTGGCAG CCAACTGTGC TTCAGCACTG GCGCAGATGG CAGCGGCTTC ATGTGTTCAG
    GAGGACAAAG AGGAGAAAGA ATTAGGAGAC ACTGGAGATG CCATTACTCA AGTGAAGCAG
    CGTGTGGAAC AGAAGCTGGA GCAGATGAGC CGTTCTCAGG GCGTGAGGCT TCACACTGCT
    CATGTCCTCT GCATGGACGC CATTCTCAAC GTGGGCCTGG AAATGGGCAG CCATAACCAC
    GACTGCTGGC CTCACGTCTT CAGGGTGATC GAGTATGTGA GTACACTGGA GCACACTCAC
    TTCAGTGACG GCGGCTCTCA GCCTCCTCTG GCCATCACGC AGGCGCAGCA GGCTGTAGCC
    GTGGATCTGG ATCTGGGTCT GGATCTGAGC GCAGAGGTCA GTCCGGAGCT GGAGCTGAGC
    CTCAGTCAGC CTGTCATCCA GCCGCTCTCC ATCCAGCAGC TCCTGAAGGA CAGCGCTCGC
    GGGAAAGCCT TGGATCTGCG GGGCGGGAGT CTGCTCACGG GAACCAGCGC AGCCAAAGCC
    GTCTGCACAC TTTCCACACA AGCTGACAGG CTGTTTGAGG ACGCCGTCAG TAAACTCAAC
    CTCGTGAGTC TTGTGGGATT CCTGCATCAG CTCAGAAAAG CTTCACAGTC TCAGCTGTTT
    GATTCCGTCA CAGAAACTGG AGACTATTCA CTGGCCATGC CAGGAGAGGC AAAGTCGACC
    CAGGACAAGC GCAGCGCCCT GCACCTCTTC CGGCTGGGAG AAGCCATGCT GCGCATCGTG
    AAGAACAAGA GCAGGCCGCT GCTGCACATG ATGAGAGCCT GGAGCGTTGT AGCACCACAC
    CTGGTGGAGG CGGCCTGTCA CAAGGAGCGC CACGTGTCCC AGAAGGCCGT GTCCTTCATC
    CATGATGTCC TGATGGAGGT TTTGAGCAGC TGGCCGGAGC TGCCGCATTT TCACTTCAAT
    GAGGCTCTTT TCAGACCGTT TGAGCACATC ATGCAGCTGG AGCTCTGCGA TGAAGACGTC
    CAGGATCAGG TGATCACTTC CATCGGAGAG CTGGTGGAGA TGTGTTCAGC GCAGATTCAG
    TCTGGATGGA GGCCGCTCTT CAGCGCTCTC AGAACCGTGC ATGGAAACAA GACGGATGTC
    AAGGATTATC TGATAGGAGA ATATTCTATG GGGAAGTCTC AGGCTCCAGT GTTTGATGTG
    TTTGAAGCCT TCATTAACAC AGACAACATT CAGGTGTTTG CTAACGCCGC CACCGACTAC
    ATCATGTGCC TTATGAAGTT TGTCAAAGGT TTAGGTGAAG TGGACTATAA GGAGATTGGC
    GATTGTGTTC ATGTCAGTGG ATACAGCTCT ACAGATCTGT GTCTGCCTGC GCTCGATTAC
    CTGAGGAAGT GCTCACAGCT GCTTGCCAAG ATCTATAAGA TGCCATCAAA GCCTGTGTTT
    CTGGGTGCGC GGCTGGCGAG TCTCCCAATG AGAGTGCAGG AGAGATCCGT GAGCAGCGAA
    GACGGCTTGG ACTGTGTGCT AGCCGAGTTT GATGACGATA CAGGTCTGAT TCAGGTGTGG
    ATCCTGCTGT TGGAGCAGCT GACAGCCGCT GTGTCCAACT GTCCCCGACA GCATCAGCCG
    CCGACCCTCG AGCTGCTGTT TGAGCTTCTG CGGGAGGTCA CCAAAATACC AGGGCCCGGT
    TTCGCCATCT TCTCTGTCAT CCAGCTGCTT CTTCCTGTGA TGTCACTGTG GCTCCAGCGT
    AGTCATGGCG ATCACTCATA CTGGGACATC GCAGCCGCAA ACTTCAAACA CGCCATCGGA
    CTGTCCTGTG AGCTGGTGGT GGAGCACATC CACAGCTTCA TACATTCAGA TATTGGCTAT
    GAGAGTCTGA TTAACCTGAT GCTGAAAGAT CTCTTCAAAC TGCTGGTTTC CTGTGTGGCA
    GAACCGGCTG AAACCATTTC CAGAGTCGGC TGCTCCTGTA TCAGGTATGT TCTGGTAACC
    GCCGGGCCTG TTTTCACTGA GGAGATGTGG CGTTTAGCAT GCTGTGCCCT GCAGGACGCT
    TTCTCAGCCA CTTTGGAGCC TGTCAAGAAC TTGCTGGCCT GTTTCCGGAG CGGTTCAGAC
    AGTTTCACTG GAGATGCGTG TGAAGTGAAA GTGGCAGCCC CGTCACACTC TCCCTCAGCA
    GAAGCAGAGA ACATGAGGAT TCGGGCCATG GCTCAACAGG TGTTCATGCT GGATACGCAG
    TGCTCTCCTA AAACTCCCAG CAGCAAGGAT GGGTTTGAGC ATGCTCAGTC CTGCGTGCTG
    ATCATTGAGC TTCCAGCTGA CCAGCACTCC AACGGCCAAG CTCAGAAGAG AAAAATCGGG
    ATCCCGTTCA GGACTCTGGT TGTGAGTTTA CTGTCCCATC AGGTGCTCCT GCAGAATCTG
    TATGACATCC TGTTAGAGGA GTTTGTCAAA GGTTCTGAGG GCAAAATCAC GGCTGTGTCG
    GAGCCAAAAC CTGCAGGATT TCTCCGCTAC ATCTCCATGC AGAACCTGGC CATCATCTTT
    GACCTCCTGC TGGACTCGTA TCGGACTGCC CGGGAATTTG ACACCCGTCC TGGCCTGAAG
    TACCTGCTCA TGAAAGTGTC CGGAGTGTGT GGAGCTGCTA ACTTATACCG CCAGTCTGCC
    ATGAGCTTCA ATATCTACTT TCAGGCTCTG CTTTGTGCAA TGCTGACAAA CCAGGAGAAC
    ATGAGCGTGG AGCAGGTGAA GAAGATCCTG AGTGAGGAAG AGGAGGGCGG ATCAGACTCC
    TCTCAGCAGT GCTCCTCCGA GGACGAGGAT ATATTTGAAG AAATGGCTCA AGTGAGTCCT
    CCACGTGCCC GAGAGAAACG TCAGTGGCAC ACGCGTATCC CATCTCTGAG CACGCAGCCC
    GCCAGCAGTG CCGACTGGGC CTGGCTGGTA AAACGTCTGC ACAAACTCTG CATGGACTTG
    TGCAACAACT ACATCCAGAT GCACCTGGAC CTGGAGAGTA CTTTGGGAGA GCAACCCACC
    CATCGTGCTG ACCCACTCTT TTTCTTGCCC CTTTTTAATT CTGAGACTTC CACGCCCTCA
    ACTGGTGGCC TGTCTGGCCA CGGGACTCCT TCAGAAGACA GTTTCCGCCT TAACCTGGAT
    GAGACGCCCT CAGAAGACGT TCATAGTCCC GGCGGGTCAA ACGCAAGCTT TCCCCAGCGG
    CGGGACGGGG GCCGCAAGAA GGAATGGTGG GAGAGTGCTG GAAACAAGCT GTACACCATA
    GCCACCGACA AGACCATTAC CAAACTGATG ATCGAGTACA AGAAACGCAA GCAGCAGCAG
    CAGCACAATC ACACCATGTT TGCCAAAGAT GTGAAGGCAG GAGAAAAGAG GGGCGAGCCT
    GTGACCCTAC GAGGCCCGGA CTCGCCCGGA CCGCAGAGGC CCCAACAGCT GGTTGAACAG
    GGGCCCATGA GGCATTCCTT TAGTGCGGGA CCCGAGCTGC TGCGGCAGGA GAAAAGACCA
    CGATCGGGAT CCACAGCCAG TTCACACAAC ATCTCTCTCA GAGATTCTGA GGCGCAGATA
    CAGGCCTGGA CAAACATGGT CCTGACCATC CTGAATCAGA TCCAGCTTCT GCCGGACCCC
    ACGTTCATGG CTCTGCAGCC GGCGGTGTTT CCCTGCATCA GTCAGCTGAC CTGTCATGTG
    ACCGAGCTGC GCGTGCGGCA GGCGGTGCGT GAGTGGCTGG GCCGAGTGGG ACGCGTTTAT
    GACATCATCC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43858
    Clone ID Related Accession (Same CDS sequence) XM_009307061.3 , XM_009307061.2
    Accession Version XM_009307061.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307061.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAGGAGA TTCTGCGGAG GCTTCAGAAG GAGGCGTCGG GCAGTAAATA CAGGGCGATC 
    CGGGACTCCT GCGGTGTGGC GTGCGAAACT CTTCAGGCTC AGAATGGATC AGTTAAAATA
    TCACCATCTA AACTCAGAGA GAAGTGTTTA TTCCCTCTTC AGTTGGCGCT GGAGTCTAAA
    AACACCAAAC TGGCACAGAC GGCCCTCACT GGGATGCAGA AGATCCTGTG TGAGGATTGT
    TTTCTCGCGG TGGAAGCAGA AGCTCCAGAG AAACAACTGT TGGGTCAAAT CCTGGAAGCT
    GTCAGAGTGA CGCCGAGCCT CCATGAAGAC CTGCAGGTGG AGGTTATGAA GGTCCTACTT
    TGCGTCACCT ACTCCTCGAC CTTCGAGATC ACTGGCCGCA ACATCTTGAA AATTGCTGAG
    GTATGTATAG AGACGTATAT ATCCAGTTGT CATCAGCGCA GTATCAACAC GGCGGTCAGA
    GCCACTCTCA GCCAGATTCT TGGAGACCTC ACTCTACAGC TACGCCATAG ACAAGAAAAC
    ACAGATGTTG ATGGTGAAGA ACAGTGTATT CCTGTGCACC AGCGCAAAGA TGTCTCTCCC
    AGTACTGAGG CGCTGTATGA AGATGTTGTC ATGGTCCTCA CTGTTTTCTG TGACAAACTG
    GAGTCCGTCT CAAATGAAAA CCAGCTGCTG CAGCTGTTGT ACCTGGAGTC TATTTTGTCC
    ATGCTCAGCA GCTGTCCTCC CACAATGCAC CTGCAGCGAA ACTTCACTGA CCTAGTCTGG
    AAGCAGTTAT GTCCCACCCT GGTGGTGATA ATGGGAAACC CAGCGAGTGA TAAGAGCATC
    ACCTCTGCTC ATGGCGCTCA GTGTCAGGAC TCAGTATCAG CCCCGGGGTC AGCGGTTCTG
    GATCAGGGCA GGGGTTCGGG ATGCTCCTCT AGTGCTCCGC TTATGATTGG GCCGGTGGTC
    CGGACCATCT GCTATGTGGC TGCAGAGTTG GTGCGTCTGG TAGGCTGTGT CGAGTCCATG
    AAACCTGTTT TGCAGTCTCT GTACCACCGT ATTCTGCTCT ACCCGCCACC CCAACACAGA
    GTGGAGGCCA TTAAGATTAT GAAAGAGATT GTGGGAAGCC CCCAGCGCTT GTTTGACCTC
    GCCGGTCCAT GCGTGATTGA ACACGAGACG AGAAAGAGGT CTTTCTCCAA AAGGAAATCA
    CATCTCGATC TTCTCAAACT CGTAATGGAT GGCATCACAG AAGCGTGTAT GAAGGGTGGG
    ATTGAGGCCT GTTACTCCTC CGTCTCCTGC GCCGGGGCCC TATTAGGTGC CTTGGATGAG
    CTCAGTCAGG GGCGTGGCCT GCAGCAGGAC CAGACCCGCA TGCTGCTGCG CCGATTGGAG
    GAGCTCAAGG AAGGGGCGGA GTCAATGCGT GAGTCTGTGG AAGTCAACGA AGCGGACTTC
    CGCTGGCAGC GGCGAGTGCT CTCATCCGAA AACGCTGCAT GGGAGGCGAG CATTACCGAA
    CGCAGCCCTG ACATTAGCAT TAGCGTCACC ACCGACACAG GCCAGACCAC TTTGGAAGGG
    GATGTAGGGC AGACTACGCT TGAAGGGGAT ATGGGGAGGA CCACTCCTGA AGATTGCGGG
    GACGAACCGC GACTACCTTC GCCTACCTGT GGATCTCGTC AGGAGTGTGT CGCACCTGAA
    ATCTCATCCT CTACGGCTCC TCCTGACGTA GTGCAACGCA GCCATGGCCT AGCCTATCCT
    GACATCACCA ACTTCTTATC GGTGGAGTCC CGGACGCGTT CGCATGGCTC CCGATACAGC
    GAGAGTAACT TCAGTGCCGA CGAGCAGGAG CTGTCCAGAA CAGAGTTTGA CTCCTGTGAT
    CAGTACTCCA TGGCTGCAGA AAAGGACTCC GGACGGTCTG ATGTATCCGA TATGGGCTCT
    GATAACTGCT CATTGGCTGA TGAGGAGCAG ACGCCGCGGG ACTGTCCCGG CCACCGTACA
    CTGCGGACTG CAGCTTTGTC TTTAAAGCTG CTGAAGAACC AGGAGGCGGA CCAACAGAGC
    GCAAGGCTCT TTGTGCAGTC TCTAGCCACA CTTCTGCCCC GTCTGCTGGG ACTGCACAGC
    ACCACAGATG TAGATGCGAC CCTGCAGAAC TTCTCCTCCA CCTTCTGCTC GGGACTACAA
    GCAGGTGGGG TTCATTCTCC AGGTTTCGAG GGCAATGAAA ACCTGAACTG TCAGGCTCTA
    ATGAACGCAG ACGGATTGTA CCTGGTCTCC TATTATGCTC TCCTGCTCAA CCTCAAGCTG
    TGCTGCTGTG ACTATTACCA CAGAAAGCCC ACGTCTGCTC TGGTGTCTCT GAAGGAGTTT
    GTGCGTCAGA TTCAGAGCAG TGGGGTTCTG GTGGTCTTGT CTCAGGCCTG GATAGAGGAG
    CTTTATAACC AGGTTTTGGA GAGAAACCTG CTGGGAGAAG CGGGATACTG GGGCTCGGCT
    GAGGAGCAGT CTTTACCCCT CATCACCATG CTCACTGATA TTGATGGACT GGGCAGCAGC
    GCTATTGGTG GTCAGCTGAT CAGAAAAGCC TCATCACAGT CACCCTTCAC CTGTGACAAA
    AACGGCAATG ACAGCCTGGT GGCAGGTGTG GTTTTTGCGC GTTACATCCT GACCGGCTGC
    TGGAAAAACC TGATCGACAC ACTGTCCACG CCGCTCACAG GGCGCATGGC TGGAAGCTCT
    CGCAGTCTGG CTTTCATTCT GGGGGCAGAG GGCGTTAAAG AGCAGACGCA GAGGGAAAGA
    GACACCATCT GCCTCAGTCT GGACGGTCTG CGCAAGGCTG CAGCGCTCAG CTGCGCTCTG
    GGGGTGGCAG CCAACTGTGC TTCAGCACTG GCGCAGATGG CAGCGGCTTC ATGTGTTCAG
    GAGGACAAAG AGGAGAAAGA ATTAGGAGAC ACTGGAGATG CCATTACTCA AGTGAAGCAG
    CGTGTGGAAC AGAAGCTGGA GCAGATGAGC CGTTCTCAGG GCGTGAGGCT TCACACTGCT
    CATGTCCTCT GCATGGACGC CATTCTCAAC GTGGGCCTGG AAATGGGCAG CCATAACCAC
    GACTGCTGGC CTCACGTCTT CAGGGTGATC GAGTATGTGA GTACACTGGA GCACACTCAC
    TTCAGTGACG GCGGCTCTCA GCCTCCTCTG GCCATCACGC AGGCGCAGCA GGCTGTAGCC
    GTGGATCTGG ATCTGGGTCT GGATCTGAGC GCAGAGGTCA GTCCGGAGCT GGAGCTGAGC
    CTCAGTCAGC CTGTCATCCA GCCGCTCTCC ATCCAGCAGC TCCTGAAGGA CAGCGCTCGC
    GGGAAAGCCT TGGATCTGCG GGGCGGGAGT CTGCTCACGG GAACCAGCGC AGCCAAAGCC
    GTCTGCACAC TTTCCACACA AGCTGACAGG CTGTTTGAGG ACGCCGTCAG TAAACTCAAC
    CTCGTGAGTC TTGTGGGATT CCTGCATCAG CTCAGAAAAG CTTCACAGTC TCAGCTGTTT
    GATTCCGTCA CAGAAACTGG AGACTATTCA CTGGCCATGC CAGGAGAGGC AAAGTCGACC
    CAGGACAAGC GCAGCGCCCT GCACCTCTTC CGGCTGGGAG AAGCCATGCT GCGCATCGTG
    AAGAACAAGA GCAGGCCGCT GCTGCACATG ATGAGAGCCT GGAGCGTTGT AGCACCACAC
    CTGGTGGAGG CGGCCTGTCA CAAGGAGCGC CACGTGTCCC AGAAGGCCGT GTCCTTCATC
    CATGATGTCC TGATGGAGGT TTTGAGCAGC TGGCCGGAGC TGCCGCATTT TCACTTCAAT
    GAGGCTCTTT TCAGACCGTT TGAGCACATC ATGCAGCTGG AGCTCTGCGA TGAAGACGTC
    CAGGATCAGG TGATCACTTC CATCGGAGAG CTGGTGGAGA TGTGTTCAGC GCAGATTCAG
    TCTGGATGGA GGCCGCTCTT CAGCGCTCTC AGAACCGTGC ATGGAAACAA GACGGATGTC
    AAGGATTATC TGATAGGAGA ATATTCTATG GGGAAGTCTC AGGCTCCAGT GTTTGATGTG
    TTTGAAGCCT TCATTAACAC AGACAACATT CAGGTGTTTG CTAACGCCGC CACCGACTAC
    ATCATGTGCC TTATGAAGTT TGTCAAAGGT TTAGGTGAAG TGGACTATAA GGAGATTGGC
    GATTGTGTTC ATGTCAGTGG ATACAGCTCT ACAGATCTGT GTCTGCCTGC GCTCGATTAC
    CTGAGGAAGT GCTCACAGCT GCTTGCCAAG ATCTATAAGA TGCCATCAAA GCCTGTGTTT
    CTGGGTGCGC GGCTGGCGAG TCTCCCAATG AGAGTGCAGG AGAGATCCGT GAGCAGCGAA
    GACGGCTTGG ACTGTGTGCT AGCCGAGTTT GATGACGATA CAGGTCTGAT TCAGGTGTGG
    ATCCTGCTGT TGGAGCAGCT GACAGCCGCT GTGTCCAACT GTCCCCGACA GCATCAGCCG
    CCGACCCTCG AGCTGCTGTT TGAGCTTCTG CGGGAGGTCA CCAAAATACC AGGGCCCGGT
    TTCGCCATCT TCTCTGTCAT CCAGCTGCTT CTTCCTGTGA TGTCACTGTG GCTCCAGCGT
    AGTCATGGCG ATCACTCATA CTGGGACATC GCAGCCGCAA ACTTCAAACA CGCCATCGGA
    CTGTCCTGTG AGCTGGTGGT GGAGCACATC CACAGCTTCA TACATTCAGA TATTGGCTAT
    GAGAGTCTGA TTAACCTGAT GCTGAAAGAT CTCTTCAAAC TGCTGGTTTC CTGTGTGGCA
    GAACCGGCTG AAACCATTTC CAGAGTCGGC TGCTCCTGTA TCAGGTATGT TCTGGTAACC
    GCCGGGCCTG TTTTCACTGA GGAGATGTGG CGTTTAGCAT GCTGTGCCCT GCAGGACGCT
    TTCTCAGCCA CTTTGGAGCC TGTCAAGAAC TTGCTGGCCT GTTTCCGGAG CGGTTCAGAC
    AGTTTCACTG GAGATGCGTG TGAAGTGAAA GTGGCAGCCC CGTCACACTC TCCCTCAGCA
    GAAGCAGAGA ACATGAGGAT TCGGGCCATG GCTCAACAGG TGTTCATGCT GGATACGCAG
    TGCTCTCCTA AAACTCCCAG CAGCAAGGAT GGGTTTGAGC ATGCTCAGTC CTGCGTGCTG
    ATCATTGAGC TTCCAGCTGA CCAGCACTCC AACGGCCAAG CTCAGAAGAG GATCCCGTTC
    AGGACTCTGG TTGTGAGTTT ACTGTCCCAT CAGGTGCTCC TGCAGAATCT GTATGACATC
    CTGTTAGAGG AGTTTGTCAA AGGTTCTGAG GGCAAAATCA CGGCTGTGTC GGAGCCAAAA
    CCTGCAGGAT TTCTCCGCTA CATCTCCATG CAGAACCTGG CCATCATCTT TGACCTCCTG
    CTGGACTCGT ATCGGACTGC CCGGGAATTT GACACCCGTC CTGGCCTGAA GTACCTGCTC
    ATGAAAGTGT CCGGAGTGTG TGGAGCTGCT AACTTATACC GCCAGTCTGC CATGAGCTTC
    AATATCTACT TTCAGGCTCT GCTTTGTGCA ATGCTGACAA ACCAGGAGAA CATGAGCGTG
    GAGCAGGTGA AGAAGATCCT GAGTGAGGAA GAGGAGGGCG GATCAGACTC CTCTCAGCAG
    TGCTCCTCCG AGGACGAGGA TATATTTGAA GAAATGGCTC AAGTGAGTCC TCCACGTGCC
    CGAGAGAAAC GTCAGTGGCA CACGCGTATC CCATCTCTGA GCACGCAGCC CGCCAGCAGT
    GCCGACTGGG CCTGGCTGGT AAAACGTCTG CACAAACTCT GCATGGACTT GTGCAACAAC
    TACATCCAGA TGCACCTGGA CCTGGAGAGT ACTTTGGGAG AGCAACCCAC CCATCGTGCT
    GACCCACTCT TTTTCTTGCC CCTTTTTAAT TCTGAGACTT CCACGCCCTC AACTGGTGGC
    CTGTCTGGCC ACGGGACTCC TTCAGAAGAC AGTTTCCGCC TTAACCTGGA TGAGACGCCC
    TCAGAAGACG TTCATAGTCC CGGCGGGTCA AACGCAAGCT TTCCCCAGCG GCGGGACGGG
    GGCCGCAAGA AGGAATGGTG GGAGAGTGCT GGAAACAAGC TGTACACCAT AGCCACCGAC
    AAGACCATTA CCAAACTGAT GATCGAGTAC AAGAAACGCA AGCAGCAGCA GCAGCACAAT
    CACACCATGT TTGCCAAAGA TGTGAAGGCA GGAGAAAAGA GGGGCGAGCC TGTGACCCTA
    CGAGGCCCGG ACTCGCCCGG ACCGCAGAGG CCCCAACAGC TGGTTGAACA GGGGCCCATG
    AGGCATTCCT TTAGTGCGGG ACCCGAGCTG CTGCGGCAGG AGAAAAGACC ACGATCGGGA
    TCCACAGCCA GTTCACACAA CATCTCTCTC AGAGATTCTG AGGCGCAGAT ACAGGCCTGG
    ACAAACATGG TCCTGACCAT CCTGAATCAG ATCCAGCTTC TGCCGGACCC CACGTTCATG
    GCTCTGCAGC CGGCGGTGTT TCCCTGCATC AGTCAGCTGA CCTGTCATGT GACCGAGCTG
    CGCGTGCGGC AGGCGGTGCG TGAGTGGCTG GGCCGAGTGG GACGCGTTTA TGACATCATC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305336.1
    CDS329..6874
    Translation

    Target ORF information:

    RefSeq Version XM_009307061.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ARFGEF family member 3 (arfgef3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307061.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAGGAGA TTCTGCGGAG GCTTCAGAAG GAGGCGTCGG GCAGTAAATA CAGGGCGATC 
    CGGGACTCCT GCGGTGTGGC GTGCGAAACT CTTCAGGCTC AGAATGGATC AGTTAAAATA
    TCACCATCTA AACTCAGAGA GAAGTGTTTA TTCCCTCTTC AGTTGGCGCT GGAGTCTAAA
    AACACCAAAC TGGCACAGAC GGCCCTCACT GGGATGCAGA AGATCCTGTG TGAGGATTGT
    TTTCTCGCGG TGGAAGCAGA AGCTCCAGAG AAACAACTGT TGGGTCAAAT CCTGGAAGCT
    GTCAGAGTGA CGCCGAGCCT CCATGAAGAC CTGCAGGTGG AGGTTATGAA GGTCCTACTT
    TGCGTCACCT ACTCCTCGAC CTTCGAGATC ACTGGCCGCA ACATCTTGAA AATTGCTGAG
    GTATGTATAG AGACGTATAT ATCCAGTTGT CATCAGCGCA GTATCAACAC GGCGGTCAGA
    GCCACTCTCA GCCAGATTCT TGGAGACCTC ACTCTACAGC TACGCCATAG ACAAGAAAAC
    ACAGATGTTG ATGGTGAAGA ACAGTGTATT CCTGTGCACC AGCGCAAAGA TGTCTCTCCC
    AGTACTGAGG CGCTGTATGA AGATGTTGTC ATGGTCCTCA CTGTTTTCTG TGACAAACTG
    GAGTCCGTCT CAAATGAAAA CCAGCTGCTG CAGCTGTTGT ACCTGGAGTC TATTTTGTCC
    ATGCTCAGCA GCTGTCCTCC CACAATGCAC CTGCAGCGAA ACTTCACTGA CCTAGTCTGG
    AAGCAGTTAT GTCCCACCCT GGTGGTGATA ATGGGAAACC CAGCGAGTGA TAAGAGCATC
    ACCTCTGCTC ATGGCGCTCA GTGTCAGGAC TCAGTATCAG CCCCGGGGTC AGCGGTTCTG
    GATCAGGGCA GGGGTTCGGG ATGCTCCTCT AGTGCTCCGC TTATGATTGG GCCGGTGGTC
    CGGACCATCT GCTATGTGGC TGCAGAGTTG GTGCGTCTGG TAGGCTGTGT CGAGTCCATG
    AAACCTGTTT TGCAGTCTCT GTACCACCGT ATTCTGCTCT ACCCGCCACC CCAACACAGA
    GTGGAGGCCA TTAAGATTAT GAAAGAGATT GTGGGAAGCC CCCAGCGCTT GTTTGACCTC
    GCCGGTCCAT GCGTGATTGA ACACGAGACG AGAAAGAGGT CTTTCTCCAA AAGGAAATCA
    CATCTCGATC TTCTCAAACT CGTAATGGAT GGCATCACAG AAGCGTGTAT GAAGGGTGGG
    ATTGAGGCCT GTTACTCCTC CGTCTCCTGC GCCGGGGCCC TATTAGGTGC CTTGGATGAG
    CTCAGTCAGG GGCGTGGCCT GCAGCAGGAC CAGACCCGCA TGCTGCTGCG CCGATTGGAG
    GAGCTCAAGG AAGGGGCGGA GTCAATGCGT GAGTCTGTGG AAGTCAACGA AGCGGACTTC
    CGCTGGCAGC GGCGAGTGCT CTCATCCGAA AACGCTGCAT GGGAGGCGAG CATTACCGAA
    CGCAGCCCTG ACATTAGCAT TAGCGTCACC ACCGACACAG GCCAGACCAC TTTGGAAGGG
    GATGTAGGGC AGACTACGCT TGAAGGGGAT ATGGGGAGGA CCACTCCTGA AGATTGCGGG
    GACGAACCGC GACTACCTTC GCCTACCTGT GGATCTCGTC AGGAGTGTGT CGCACCTGAA
    ATCTCATCCT CTACGGCTCC TCCTGACGTA GTGCAACGCA GCCATGGCCT AGCCTATCCT
    GACATCACCA ACTTCTTATC GGTGGAGTCC CGGACGCGTT CGCATGGCTC CCGATACAGC
    GAGAGTAACT TCAGTGCCGA CGAGCAGGAG CTGTCCAGAA CAGAGTTTGA CTCCTGTGAT
    CAGTACTCCA TGGCTGCAGA AAAGGACTCC GGACGGTCTG ATGTATCCGA TATGGGCTCT
    GATAACTGCT CATTGGCTGA TGAGGAGCAG ACGCCGCGGG ACTGTCCCGG CCACCGTACA
    CTGCGGACTG CAGCTTTGTC TTTAAAGCTG CTGAAGAACC AGGAGGCGGA CCAACAGAGC
    GCAAGGCTCT TTGTGCAGTC TCTAGCCACA CTTCTGCCCC GTCTGCTGGG ACTGCACAGC
    ACCACAGATG TAGATGCGAC CCTGCAGAAC TTCTCCTCCA CCTTCTGCTC GGGACTACAA
    GCAGGTGGGG TTCATTCTCC AGGTTTCGAG GGCAATGAAA ACCTGAACTG TCAGGCTCTA
    ATGAACGCAG ACGGATTGTA CCTGGTCTCC TATTATGCTC TCCTGCTCAA CCTCAAGCTG
    TGCTGCTGTG ACTATTACCA CAGAAAGCCC ACGTCTGCTC TGGTGTCTCT GAAGGAGTTT
    GTGCGTCAGA TTCAGAGCAG TGGGGTTCTG GTGGTCTTGT CTCAGGCCTG GATAGAGGAG
    CTTTATAACC AGGTTTTGGA GAGAAACCTG CTGGGAGAAG CGGGATACTG GGGCTCGGCT
    GAGGAGCAGT CTTTACCCCT CATCACCATG CTCACTGATA TTGATGGACT GGGCAGCAGC
    GCTATTGGTG GTCAGCTGAT CAGAAAAGCC TCATCACAGT CACCCTTCAC CTGTGACAAA
    AACGGCAATG ACAGCCTGGT GGCAGGTGTG GTTTTTGCGC GTTACATCCT GACCGGCTGC
    TGGAAAAACC TGATCGACAC ACTGTCCACG CCGCTCACAG GGCGCATGGC TGGAAGCTCT
    CGCAGTCTGG CTTTCATTCT GGGGGCAGAG GGCGTTAAAG AGCAGACGCA GAGGGAAAGA
    GACACCATCT GCCTCAGTCT GGACGGTCTG CGCAAGGCTG CAGCGCTCAG CTGCGCTCTG
    GGGGTGGCAG CCAACTGTGC TTCAGCACTG GCGCAGATGG CAGCGGCTTC ATGTGTTCAG
    GAGGACAAAG AGGAGAAAGA ATTAGGAGAC ACTGGAGATG CCATTACTCA AGTGAAGCAG
    CGTGTGGAAC AGAAGCTGGA GCAGATGAGC CGTTCTCAGG GCGTGAGGCT TCACACTGCT
    CATGTCCTCT GCATGGACGC CATTCTCAAC GTGGGCCTGG AAATGGGCAG CCATAACCAC
    GACTGCTGGC CTCACGTCTT CAGGGTGATC GAGTATGTGA GTACACTGGA GCACACTCAC
    TTCAGTGACG GCGGCTCTCA GCCTCCTCTG GCCATCACGC AGGCGCAGCA GGCTGTAGCC
    GTGGATCTGG ATCTGGGTCT GGATCTGAGC GCAGAGGTCA GTCCGGAGCT GGAGCTGAGC
    CTCAGTCAGC CTGTCATCCA GCCGCTCTCC ATCCAGCAGC TCCTGAAGGA CAGCGCTCGC
    GGGAAAGCCT TGGATCTGCG GGGCGGGAGT CTGCTCACGG GAACCAGCGC AGCCAAAGCC
    GTCTGCACAC TTTCCACACA AGCTGACAGG CTGTTTGAGG ACGCCGTCAG TAAACTCAAC
    CTCGTGAGTC TTGTGGGATT CCTGCATCAG CTCAGAAAAG CTTCACAGTC TCAGCTGTTT
    GATTCCGTCA CAGAAACTGG AGACTATTCA CTGGCCATGC CAGGAGAGGC AAAGTCGACC
    CAGGACAAGC GCAGCGCCCT GCACCTCTTC CGGCTGGGAG AAGCCATGCT GCGCATCGTG
    AAGAACAAGA GCAGGCCGCT GCTGCACATG ATGAGAGCCT GGAGCGTTGT AGCACCACAC
    CTGGTGGAGG CGGCCTGTCA CAAGGAGCGC CACGTGTCCC AGAAGGCCGT GTCCTTCATC
    CATGATGTCC TGATGGAGGT TTTGAGCAGC TGGCCGGAGC TGCCGCATTT TCACTTCAAT
    GAGGCTCTTT TCAGACCGTT TGAGCACATC ATGCAGCTGG AGCTCTGCGA TGAAGACGTC
    CAGGATCAGG TGATCACTTC CATCGGAGAG CTGGTGGAGA TGTGTTCAGC GCAGATTCAG
    TCTGGATGGA GGCCGCTCTT CAGCGCTCTC AGAACCGTGC ATGGAAACAA GACGGATGTC
    AAGGATTATC TGATAGGAGA ATATTCTATG GGGAAGTCTC AGGCTCCAGT GTTTGATGTG
    TTTGAAGCCT TCATTAACAC AGACAACATT CAGGTGTTTG CTAACGCCGC CACCGACTAC
    ATCATGTGCC TTATGAAGTT TGTCAAAGGT TTAGGTGAAG TGGACTATAA GGAGATTGGC
    GATTGTGTTC ATGTCAGTGG ATACAGCTCT ACAGATCTGT GTCTGCCTGC GCTCGATTAC
    CTGAGGAAGT GCTCACAGCT GCTTGCCAAG ATCTATAAGA TGCCATCAAA GCCTGTGTTT
    CTGGGTGCGC GGCTGGCGAG TCTCCCAATG AGAGTGCAGG AGAGATCCGT GAGCAGCGAA
    GACGGCTTGG ACTGTGTGCT AGCCGAGTTT GATGACGATA CAGGTCTGAT TCAGGTGTGG
    ATCCTGCTGT TGGAGCAGCT GACAGCCGCT GTGTCCAACT GTCCCCGACA GCATCAGCCG
    CCGACCCTCG AGCTGCTGTT TGAGCTTCTG CGGGAGGTCA CCAAAATACC AGGGCCCGGT
    TTCGCCATCT TCTCTGTCAT CCAGCTGCTT CTTCCTGTGA TGTCACTGTG GCTCCAGCGT
    AGTCATGGCG ATCACTCATA CTGGGACATC GCAGCCGCAA ACTTCAAACA CGCCATCGGA
    CTGTCCTGTG AGCTGGTGGT GGAGCACATC CACAGCTTCA TACATTCAGA TATTGGCTAT
    GAGAGTCTGA TTAACCTGAT GCTGAAAGAT CTCTTCAAAC TGCTGGTTTC CTGTGTGGCA
    GAACCGGCTG AAACCATTTC CAGAGTCGGC TGCTCCTGTA TCAGGTATGT TCTGGTAACC
    GCCGGGCCTG TTTTCACTGA GGAGATGTGG CGTTTAGCAT GCTGTGCCCT GCAGGACGCT
    TTCTCAGCCA CTTTGGAGCC TGTCAAGAAC TTGCTGGCCT GTTTCCGGAG CGGTTCAGAC
    AGTTTCACTG GAGATGCGTG TGAAGTGAAA GTGGCAGCCC CGTCACACTC TCCCTCAGCA
    GAAGCAGAGA ACATGAGGAT TCGGGCCATG GCTCAACAGG TGTTCATGCT GGATACGCAG
    TGCTCTCCTA AAACTCCCAG CAGCAAGGAT GGGTTTGAGC ATGCTCAGTC CTGCGTGCTG
    ATCATTGAGC TTCCAGCTGA CCAGCACTCC AACGGCCAAG CTCAGAAGAG GATCCCGTTC
    AGGACTCTGG TTGTGAGTTT ACTGTCCCAT CAGGTGCTCC TGCAGAATCT GTATGACATC
    CTGTTAGAGG AGTTTGTCAA AGGTTCTGAG GGCAAAATCA CGGCTGTGTC GGAGCCAAAA
    CCTGCAGGAT TTCTCCGCTA CATCTCCATG CAGAACCTGG CCATCATCTT TGACCTCCTG
    CTGGACTCGT ATCGGACTGC CCGGGAATTT GACACCCGTC CTGGCCTGAA GTACCTGCTC
    ATGAAAGTGT CCGGAGTGTG TGGAGCTGCT AACTTATACC GCCAGTCTGC CATGAGCTTC
    AATATCTACT TTCAGGCTCT GCTTTGTGCA ATGCTGACAA ACCAGGAGAA CATGAGCGTG
    GAGCAGGTGA AGAAGATCCT GAGTGAGGAA GAGGAGGGCG GATCAGACTC CTCTCAGCAG
    TGCTCCTCCG AGGACGAGGA TATATTTGAA GAAATGGCTC AAGTGAGTCC TCCACGTGCC
    CGAGAGAAAC GTCAGTGGCA CACGCGTATC CCATCTCTGA GCACGCAGCC CGCCAGCAGT
    GCCGACTGGG CCTGGCTGGT AAAACGTCTG CACAAACTCT GCATGGACTT GTGCAACAAC
    TACATCCAGA TGCACCTGGA CCTGGAGAGT ACTTTGGGAG AGCAACCCAC CCATCGTGCT
    GACCCACTCT TTTTCTTGCC CCTTTTTAAT TCTGAGACTT CCACGCCCTC AACTGGTGGC
    CTGTCTGGCC ACGGGACTCC TTCAGAAGAC AGTTTCCGCC TTAACCTGGA TGAGACGCCC
    TCAGAAGACG TTCATAGTCC CGGCGGGTCA AACGCAAGCT TTCCCCAGCG GCGGGACGGG
    GGCCGCAAGA AGGAATGGTG GGAGAGTGCT GGAAACAAGC TGTACACCAT AGCCACCGAC
    AAGACCATTA CCAAACTGAT GATCGAGTAC AAGAAACGCA AGCAGCAGCA GCAGCACAAT
    CACACCATGT TTGCCAAAGA TGTGAAGGCA GGAGAAAAGA GGGGCGAGCC TGTGACCCTA
    CGAGGCCCGG ACTCGCCCGG ACCGCAGAGG CCCCAACAGC TGGTTGAACA GGGGCCCATG
    AGGCATTCCT TTAGTGCGGG ACCCGAGCTG CTGCGGCAGG AGAAAAGACC ACGATCGGGA
    TCCACAGCCA GTTCACACAA CATCTCTCTC AGAGATTCTG AGGCGCAGAT ACAGGCCTGG
    ACAAACATGG TCCTGACCAT CCTGAATCAG ATCCAGCTTC TGCCGGACCC CACGTTCATG
    GCTCTGCAGC CGGCGGTGTT TCCCTGCATC AGTCAGCTGA CCTGTCATGT GACCGAGCTG
    CGCGTGCGGC AGGCGGTGCG TGAGTGGCTG GGCCGAGTGG GACGCGTTTA TGACATCATC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43858
    Clone ID Related Accession (Same CDS sequence) XM_009307061.3 , XM_009307061.2
    Accession Version XM_009307061.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product brefeldin A-inhibited guanine nucleotide-exchange protein 3 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307061.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAGGAGA TTCTGCGGAG GCTTCAGAAG GAGGCGTCGG GCAGTAAATA CAGGGCGATC 
    CGGGACTCCT GCGGTGTGGC GTGCGAAACT CTTCAGGCTC AGAATGGATC AGTTAAAATA
    TCACCATCTA AACTCAGAGA GAAGTGTTTA TTCCCTCTTC AGTTGGCGCT GGAGTCTAAA
    AACACCAAAC TGGCACAGAC GGCCCTCACT GGGATGCAGA AGATCCTGTG TGAGGATTGT
    TTTCTCGCGG TGGAAGCAGA AGCTCCAGAG AAACAACTGT TGGGTCAAAT CCTGGAAGCT
    GTCAGAGTGA CGCCGAGCCT CCATGAAGAC CTGCAGGTGG AGGTTATGAA GGTCCTACTT
    TGCGTCACCT ACTCCTCGAC CTTCGAGATC ACTGGCCGCA ACATCTTGAA AATTGCTGAG
    GTATGTATAG AGACGTATAT ATCCAGTTGT CATCAGCGCA GTATCAACAC GGCGGTCAGA
    GCCACTCTCA GCCAGATTCT TGGAGACCTC ACTCTACAGC TACGCCATAG ACAAGAAAAC
    ACAGATGTTG ATGGTGAAGA ACAGTGTATT CCTGTGCACC AGCGCAAAGA TGTCTCTCCC
    AGTACTGAGG CGCTGTATGA AGATGTTGTC ATGGTCCTCA CTGTTTTCTG TGACAAACTG
    GAGTCCGTCT CAAATGAAAA CCAGCTGCTG CAGCTGTTGT ACCTGGAGTC TATTTTGTCC
    ATGCTCAGCA GCTGTCCTCC CACAATGCAC CTGCAGCGAA ACTTCACTGA CCTAGTCTGG
    AAGCAGTTAT GTCCCACCCT GGTGGTGATA ATGGGAAACC CAGCGAGTGA TAAGAGCATC
    ACCTCTGCTC ATGGCGCTCA GTGTCAGGAC TCAGTATCAG CCCCGGGGTC AGCGGTTCTG
    GATCAGGGCA GGGGTTCGGG ATGCTCCTCT AGTGCTCCGC TTATGATTGG GCCGGTGGTC
    CGGACCATCT GCTATGTGGC TGCAGAGTTG GTGCGTCTGG TAGGCTGTGT CGAGTCCATG
    AAACCTGTTT TGCAGTCTCT GTACCACCGT ATTCTGCTCT ACCCGCCACC CCAACACAGA
    GTGGAGGCCA TTAAGATTAT GAAAGAGATT GTGGGAAGCC CCCAGCGCTT GTTTGACCTC
    GCCGGTCCAT GCGTGATTGA ACACGAGACG AGAAAGAGGT CTTTCTCCAA AAGGAAATCA
    CATCTCGATC TTCTCAAACT CGTAATGGAT GGCATCACAG AAGCGTGTAT GAAGGGTGGG
    ATTGAGGCCT GTTACTCCTC CGTCTCCTGC GCCGGGGCCC TATTAGGTGC CTTGGATGAG
    CTCAGTCAGG GGCGTGGCCT GCAGCAGGAC CAGACCCGCA TGCTGCTGCG CCGATTGGAG
    GAGCTCAAGG AAGGGGCGGA GTCAATGCGT GAGTCTGTGG AAGTCAACGA AGCGGACTTC
    CGCTGGCAGC GGCGAGTGCT CTCATCCGAA AACGCTGCAT GGGAGGCGAG CATTACCGAA
    CGCAGCCCTG ACATTAGCAT TAGCGTCACC ACCGACACAG GCCAGACCAC TTTGGAAGGG
    GATGTAGGGC AGACTACGCT TGAAGGGGAT ATGGGGAGGA CCACTCCTGA AGATTGCGGG
    GACGAACCGC GACTACCTTC GCCTACCTGT GGATCTCGTC AGGAGTGTGT CGCACCTGAA
    ATCTCATCCT CTACGGCTCC TCCTGACGTA GTGCAACGCA GCCATGGCCT AGCCTATCCT
    GACATCACCA ACTTCTTATC GGTGGAGTCC CGGACGCGTT CGCATGGCTC CCGATACAGC
    GAGAGTAACT TCAGTGCCGA CGAGCAGGAG CTGTCCAGAA CAGAGTTTGA CTCCTGTGAT
    CAGTACTCCA TGGCTGCAGA AAAGGACTCC GGACGGTCTG ATGTATCCGA TATGGGCTCT
    GATAACTGCT CATTGGCTGA TGAGGAGCAG ACGCCGCGGG ACTGTCCCGG CCACCGTACA
    CTGCGGACTG CAGCTTTGTC TTTAAAGCTG CTGAAGAACC AGGAGGCGGA CCAACAGAGC
    GCAAGGCTCT TTGTGCAGTC TCTAGCCACA CTTCTGCCCC GTCTGCTGGG ACTGCACAGC
    ACCACAGATG TAGATGCGAC CCTGCAGAAC TTCTCCTCCA CCTTCTGCTC GGGACTACAA
    GCAGGTGGGG TTCATTCTCC AGGTTTCGAG GGCAATGAAA ACCTGAACTG TCAGGCTCTA
    ATGAACGCAG ACGGATTGTA CCTGGTCTCC TATTATGCTC TCCTGCTCAA CCTCAAGCTG
    TGCTGCTGTG ACTATTACCA CAGAAAGCCC ACGTCTGCTC TGGTGTCTCT GAAGGAGTTT
    GTGCGTCAGA TTCAGAGCAG TGGGGTTCTG GTGGTCTTGT CTCAGGCCTG GATAGAGGAG
    CTTTATAACC AGGTTTTGGA GAGAAACCTG CTGGGAGAAG CGGGATACTG GGGCTCGGCT
    GAGGAGCAGT CTTTACCCCT CATCACCATG CTCACTGATA TTGATGGACT GGGCAGCAGC
    GCTATTGGTG GTCAGCTGAT CAGAAAAGCC TCATCACAGT CACCCTTCAC CTGTGACAAA
    AACGGCAATG ACAGCCTGGT GGCAGGTGTG GTTTTTGCGC GTTACATCCT GACCGGCTGC
    TGGAAAAACC TGATCGACAC ACTGTCCACG CCGCTCACAG GGCGCATGGC TGGAAGCTCT
    CGCAGTCTGG CTTTCATTCT GGGGGCAGAG GGCGTTAAAG AGCAGACGCA GAGGGAAAGA
    GACACCATCT GCCTCAGTCT GGACGGTCTG CGCAAGGCTG CAGCGCTCAG CTGCGCTCTG
    GGGGTGGCAG CCAACTGTGC TTCAGCACTG GCGCAGATGG CAGCGGCTTC ATGTGTTCAG
    GAGGACAAAG AGGAGAAAGA ATTAGGAGAC ACTGGAGATG CCATTACTCA AGTGAAGCAG
    CGTGTGGAAC AGAAGCTGGA GCAGATGAGC CGTTCTCAGG GCGTGAGGCT TCACACTGCT
    CATGTCCTCT GCATGGACGC CATTCTCAAC GTGGGCCTGG AAATGGGCAG CCATAACCAC
    GACTGCTGGC CTCACGTCTT CAGGGTGATC GAGTATGTGA GTACACTGGA GCACACTCAC
    TTCAGTGACG GCGGCTCTCA GCCTCCTCTG GCCATCACGC AGGCGCAGCA GGCTGTAGCC
    GTGGATCTGG ATCTGGGTCT GGATCTGAGC GCAGAGGTCA GTCCGGAGCT GGAGCTGAGC
    CTCAGTCAGC CTGTCATCCA GCCGCTCTCC ATCCAGCAGC TCCTGAAGGA CAGCGCTCGC
    GGGAAAGCCT TGGATCTGCG GGGCGGGAGT CTGCTCACGG GAACCAGCGC AGCCAAAGCC
    GTCTGCACAC TTTCCACACA AGCTGACAGG CTGTTTGAGG ACGCCGTCAG TAAACTCAAC
    CTCGTGAGTC TTGTGGGATT CCTGCATCAG CTCAGAAAAG CTTCACAGTC TCAGCTGTTT
    GATTCCGTCA CAGAAACTGG AGACTATTCA CTGGCCATGC CAGGAGAGGC AAAGTCGACC
    CAGGACAAGC GCAGCGCCCT GCACCTCTTC CGGCTGGGAG AAGCCATGCT GCGCATCGTG
    AAGAACAAGA GCAGGCCGCT GCTGCACATG ATGAGAGCCT GGAGCGTTGT AGCACCACAC
    CTGGTGGAGG CGGCCTGTCA CAAGGAGCGC CACGTGTCCC AGAAGGCCGT GTCCTTCATC
    CATGATGTCC TGATGGAGGT TTTGAGCAGC TGGCCGGAGC TGCCGCATTT TCACTTCAAT
    GAGGCTCTTT TCAGACCGTT TGAGCACATC ATGCAGCTGG AGCTCTGCGA TGAAGACGTC
    CAGGATCAGG TGATCACTTC CATCGGAGAG CTGGTGGAGA TGTGTTCAGC GCAGATTCAG
    TCTGGATGGA GGCCGCTCTT CAGCGCTCTC AGAACCGTGC ATGGAAACAA GACGGATGTC
    AAGGATTATC TGATAGGAGA ATATTCTATG GGGAAGTCTC AGGCTCCAGT GTTTGATGTG
    TTTGAAGCCT TCATTAACAC AGACAACATT CAGGTGTTTG CTAACGCCGC CACCGACTAC
    ATCATGTGCC TTATGAAGTT TGTCAAAGGT TTAGGTGAAG TGGACTATAA GGAGATTGGC
    GATTGTGTTC ATGTCAGTGG ATACAGCTCT ACAGATCTGT GTCTGCCTGC GCTCGATTAC
    CTGAGGAAGT GCTCACAGCT GCTTGCCAAG ATCTATAAGA TGCCATCAAA GCCTGTGTTT
    CTGGGTGCGC GGCTGGCGAG TCTCCCAATG AGAGTGCAGG AGAGATCCGT GAGCAGCGAA
    GACGGCTTGG ACTGTGTGCT AGCCGAGTTT GATGACGATA CAGGTCTGAT TCAGGTGTGG
    ATCCTGCTGT TGGAGCAGCT GACAGCCGCT GTGTCCAACT GTCCCCGACA GCATCAGCCG
    CCGACCCTCG AGCTGCTGTT TGAGCTTCTG CGGGAGGTCA CCAAAATACC AGGGCCCGGT
    TTCGCCATCT TCTCTGTCAT CCAGCTGCTT CTTCCTGTGA TGTCACTGTG GCTCCAGCGT
    AGTCATGGCG ATCACTCATA CTGGGACATC GCAGCCGCAA ACTTCAAACA CGCCATCGGA
    CTGTCCTGTG AGCTGGTGGT GGAGCACATC CACAGCTTCA TACATTCAGA TATTGGCTAT
    GAGAGTCTGA TTAACCTGAT GCTGAAAGAT CTCTTCAAAC TGCTGGTTTC CTGTGTGGCA
    GAACCGGCTG AAACCATTTC CAGAGTCGGC TGCTCCTGTA TCAGGTATGT TCTGGTAACC
    GCCGGGCCTG TTTTCACTGA GGAGATGTGG CGTTTAGCAT GCTGTGCCCT GCAGGACGCT
    TTCTCAGCCA CTTTGGAGCC TGTCAAGAAC TTGCTGGCCT GTTTCCGGAG CGGTTCAGAC
    AGTTTCACTG GAGATGCGTG TGAAGTGAAA GTGGCAGCCC CGTCACACTC TCCCTCAGCA
    GAAGCAGAGA ACATGAGGAT TCGGGCCATG GCTCAACAGG TGTTCATGCT GGATACGCAG
    TGCTCTCCTA AAACTCCCAG CAGCAAGGAT GGGTTTGAGC ATGCTCAGTC CTGCGTGCTG
    ATCATTGAGC TTCCAGCTGA CCAGCACTCC AACGGCCAAG CTCAGAAGAG GATCCCGTTC
    AGGACTCTGG TTGTGAGTTT ACTGTCCCAT CAGGTGCTCC TGCAGAATCT GTATGACATC
    CTGTTAGAGG AGTTTGTCAA AGGTTCTGAG GGCAAAATCA CGGCTGTGTC GGAGCCAAAA
    CCTGCAGGAT TTCTCCGCTA CATCTCCATG CAGAACCTGG CCATCATCTT TGACCTCCTG
    CTGGACTCGT ATCGGACTGC CCGGGAATTT GACACCCGTC CTGGCCTGAA GTACCTGCTC
    ATGAAAGTGT CCGGAGTGTG TGGAGCTGCT AACTTATACC GCCAGTCTGC CATGAGCTTC
    AATATCTACT TTCAGGCTCT GCTTTGTGCA ATGCTGACAA ACCAGGAGAA CATGAGCGTG
    GAGCAGGTGA AGAAGATCCT GAGTGAGGAA GAGGAGGGCG GATCAGACTC CTCTCAGCAG
    TGCTCCTCCG AGGACGAGGA TATATTTGAA GAAATGGCTC AAGTGAGTCC TCCACGTGCC
    CGAGAGAAAC GTCAGTGGCA CACGCGTATC CCATCTCTGA GCACGCAGCC CGCCAGCAGT
    GCCGACTGGG CCTGGCTGGT AAAACGTCTG CACAAACTCT GCATGGACTT GTGCAACAAC
    TACATCCAGA TGCACCTGGA CCTGGAGAGT ACTTTGGGAG AGCAACCCAC CCATCGTGCT
    GACCCACTCT TTTTCTTGCC CCTTTTTAAT TCTGAGACTT CCACGCCCTC AACTGGTGGC
    CTGTCTGGCC ACGGGACTCC TTCAGAAGAC AGTTTCCGCC TTAACCTGGA TGAGACGCCC
    TCAGAAGACG TTCATAGTCC CGGCGGGTCA AACGCAAGCT TTCCCCAGCG GCGGGACGGG
    GGCCGCAAGA AGGAATGGTG GGAGAGTGCT GGAAACAAGC TGTACACCAT AGCCACCGAC
    AAGACCATTA CCAAACTGAT GATCGAGTAC AAGAAACGCA AGCAGCAGCA GCAGCACAAT
    CACACCATGT TTGCCAAAGA TGTGAAGGCA GGAGAAAAGA GGGGCGAGCC TGTGACCCTA
    CGAGGCCCGG ACTCGCCCGG ACCGCAGAGG CCCCAACAGC TGGTTGAACA GGGGCCCATG
    AGGCATTCCT TTAGTGCGGG ACCCGAGCTG CTGCGGCAGG AGAAAAGACC ACGATCGGGA
    TCCACAGCCA GTTCACACAA CATCTCTCTC AGAGATTCTG AGGCGCAGAT ACAGGCCTGG
    ACAAACATGG TCCTGACCAT CCTGAATCAG ATCCAGCTTC TGCCGGACCC CACGTTCATG
    GCTCTGCAGC CGGCGGTGTT TCCCTGCATC AGTCAGCTGA CCTGTCATGT GACCGAGCTG
    CGCGTGCGGC AGGCGGTGCG TGAGTGGCTG GGCCGAGTGG GACGCGTTTA TGACATCATC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305336.1
    CDS281..6826
    Translation

    Target ORF information:

    RefSeq Version XM_009307061.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ARFGEF family member 3 (arfgef3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307061.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAGGAGA TTCTGCGGAG GCTTCAGAAG GAGGCGTCGG GCAGTAAATA CAGGGCGATC 
    CGGGACTCCT GCGGTGTGGC GTGCGAAACT CTTCAGGCTC AGAATGGATC AGTTAAAATA
    TCACCATCTA AACTCAGAGA GAAGTGTTTA TTCCCTCTTC AGTTGGCGCT GGAGTCTAAA
    AACACCAAAC TGGCACAGAC GGCCCTCACT GGGATGCAGA AGATCCTGTG TGAGGATTGT
    TTTCTCGCGG TGGAAGCAGA AGCTCCAGAG AAACAACTGT TGGGTCAAAT CCTGGAAGCT
    GTCAGAGTGA CGCCGAGCCT CCATGAAGAC CTGCAGGTGG AGGTTATGAA GGTCCTACTT
    TGCGTCACCT ACTCCTCGAC CTTCGAGATC ACTGGCCGCA ACATCTTGAA AATTGCTGAG
    GTATGTATAG AGACGTATAT ATCCAGTTGT CATCAGCGCA GTATCAACAC GGCGGTCAGA
    GCCACTCTCA GCCAGATTCT TGGAGACCTC ACTCTACAGC TACGCCATAG ACAAGAAAAC
    ACAGATGTTG ATGGTGAAGA ACAGTGTATT CCTGTGCACC AGCGCAAAGA TGTCTCTCCC
    AGTACTGAGG CGCTGTATGA AGATGTTGTC ATGGTCCTCA CTGTTTTCTG TGACAAACTG
    GAGTCCGTCT CAAATGAAAA CCAGCTGCTG CAGCTGTTGT ACCTGGAGTC TATTTTGTCC
    ATGCTCAGCA GCTGTCCTCC CACAATGCAC CTGCAGCGAA ACTTCACTGA CCTAGTCTGG
    AAGCAGTTAT GTCCCACCCT GGTGGTGATA ATGGGAAACC CAGCGAGTGA TAAGAGCATC
    ACCTCTGCTC ATGGCGCTCA GTGTCAGGAC TCAGTATCAG CCCCGGGGTC AGCGGTTCTG
    GATCAGGGCA GGGGTTCGGG ATGCTCCTCT AGTGCTCCGC TTATGATTGG GCCGGTGGTC
    CGGACCATCT GCTATGTGGC TGCAGAGTTG GTGCGTCTGG TAGGCTGTGT CGAGTCCATG
    AAACCTGTTT TGCAGTCTCT GTACCACCGT ATTCTGCTCT ACCCGCCACC CCAACACAGA
    GTGGAGGCCA TTAAGATTAT GAAAGAGATT GTGGGAAGCC CCCAGCGCTT GTTTGACCTC
    GCCGGTCCAT GCGTGATTGA ACACGAGACG AGAAAGAGGT CTTTCTCCAA AAGGAAATCA
    CATCTCGATC TTCTCAAACT CGTAATGGAT GGCATCACAG AAGCGTGTAT GAAGGGTGGG
    ATTGAGGCCT GTTACTCCTC CGTCTCCTGC GCCGGGGCCC TATTAGGTGC CTTGGATGAG
    CTCAGTCAGG GGCGTGGCCT GCAGCAGGAC CAGACCCGCA TGCTGCTGCG CCGATTGGAG
    GAGCTCAAGG AAGGGGCGGA GTCAATGCGT GAGTCTGTGG AAGTCAACGA AGCGGACTTC
    CGCTGGCAGC GGCGAGTGCT CTCATCCGAA AACGCTGCAT GGGAGGCGAG CATTACCGAA
    CGCAGCCCTG ACATTAGCAT TAGCGTCACC ACCGACACAG GCCAGACCAC TTTGGAAGGG
    GATGTAGGGC AGACTACGCT TGAAGGGGAT ATGGGGAGGA CCACTCCTGA AGATTGCGGG
    GACGAACCGC GACTACCTTC GCCTACCTGT GGATCTCGTC AGGAGTGTGT CGCACCTGAA
    ATCTCATCCT CTACGGCTCC TCCTGACGTA GTGCAACGCA GCCATGGCCT AGCCTATCCT
    GACATCACCA ACTTCTTATC GGTGGAGTCC CGGACGCGTT CGCATGGCTC CCGATACAGC
    GAGAGTAACT TCAGTGCCGA CGAGCAGGAG CTGTCCAGAA CAGAGTTTGA CTCCTGTGAT
    CAGTACTCCA TGGCTGCAGA AAAGGACTCC GGACGGTCTG ATGTATCCGA TATGGGCTCT
    GATAACTGCT CATTGGCTGA TGAGGAGCAG ACGCCGCGGG ACTGTCCCGG CCACCGTACA
    CTGCGGACTG CAGCTTTGTC TTTAAAGCTG CTGAAGAACC AGGAGGCGGA CCAACAGAGC
    GCAAGGCTCT TTGTGCAGTC TCTAGCCACA CTTCTGCCCC GTCTGCTGGG ACTGCACAGC
    ACCACAGATG TAGATGCGAC CCTGCAGAAC TTCTCCTCCA CCTTCTGCTC GGGACTACAA
    GCAGGTGGGG TTCATTCTCC AGGTTTCGAG GGCAATGAAA ACCTGAACTG TCAGGCTCTA
    ATGAACGCAG ACGGATTGTA CCTGGTCTCC TATTATGCTC TCCTGCTCAA CCTCAAGCTG
    TGCTGCTGTG ACTATTACCA CAGAAAGCCC ACGTCTGCTC TGGTGTCTCT GAAGGAGTTT
    GTGCGTCAGA TTCAGAGCAG TGGGGTTCTG GTGGTCTTGT CTCAGGCCTG GATAGAGGAG
    CTTTATAACC AGGTTTTGGA GAGAAACCTG CTGGGAGAAG CGGGATACTG GGGCTCGGCT
    GAGGAGCAGT CTTTACCCCT CATCACCATG CTCACTGATA TTGATGGACT GGGCAGCAGC
    GCTATTGGTG GTCAGCTGAT CAGAAAAGCC TCATCACAGT CACCCTTCAC CTGTGACAAA
    AACGGCAATG ACAGCCTGGT GGCAGGTGTG GTTTTTGCGC GTTACATCCT GACCGGCTGC
    TGGAAAAACC TGATCGACAC ACTGTCCACG CCGCTCACAG GGCGCATGGC TGGAAGCTCT
    CGCAGTCTGG CTTTCATTCT GGGGGCAGAG GGCGTTAAAG AGCAGACGCA GAGGGAAAGA
    GACACCATCT GCCTCAGTCT GGACGGTCTG CGCAAGGCTG CAGCGCTCAG CTGCGCTCTG
    GGGGTGGCAG CCAACTGTGC TTCAGCACTG GCGCAGATGG CAGCGGCTTC ATGTGTTCAG
    GAGGACAAAG AGGAGAAAGA ATTAGGAGAC ACTGGAGATG CCATTACTCA AGTGAAGCAG
    CGTGTGGAAC AGAAGCTGGA GCAGATGAGC CGTTCTCAGG GCGTGAGGCT TCACACTGCT
    CATGTCCTCT GCATGGACGC CATTCTCAAC GTGGGCCTGG AAATGGGCAG CCATAACCAC
    GACTGCTGGC CTCACGTCTT CAGGGTGATC GAGTATGTGA GTACACTGGA GCACACTCAC
    TTCAGTGACG GCGGCTCTCA GCCTCCTCTG GCCATCACGC AGGCGCAGCA GGCTGTAGCC
    GTGGATCTGG ATCTGGGTCT GGATCTGAGC GCAGAGGTCA GTCCGGAGCT GGAGCTGAGC
    CTCAGTCAGC CTGTCATCCA GCCGCTCTCC ATCCAGCAGC TCCTGAAGGA CAGCGCTCGC
    GGGAAAGCCT TGGATCTGCG GGGCGGGAGT CTGCTCACGG GAACCAGCGC AGCCAAAGCC
    GTCTGCACAC TTTCCACACA AGCTGACAGG CTGTTTGAGG ACGCCGTCAG TAAACTCAAC
    CTCGTGAGTC TTGTGGGATT CCTGCATCAG CTCAGAAAAG CTTCACAGTC TCAGCTGTTT
    GATTCCGTCA CAGAAACTGG AGACTATTCA CTGGCCATGC CAGGAGAGGC AAAGTCGACC
    CAGGACAAGC GCAGCGCCCT GCACCTCTTC CGGCTGGGAG AAGCCATGCT GCGCATCGTG
    AAGAACAAGA GCAGGCCGCT GCTGCACATG ATGAGAGCCT GGAGCGTTGT AGCACCACAC
    CTGGTGGAGG CGGCCTGTCA CAAGGAGCGC CACGTGTCCC AGAAGGCCGT GTCCTTCATC
    CATGATGTCC TGATGGAGGT TTTGAGCAGC TGGCCGGAGC TGCCGCATTT TCACTTCAAT
    GAGGCTCTTT TCAGACCGTT TGAGCACATC ATGCAGCTGG AGCTCTGCGA TGAAGACGTC
    CAGGATCAGG TGATCACTTC CATCGGAGAG CTGGTGGAGA TGTGTTCAGC GCAGATTCAG
    TCTGGATGGA GGCCGCTCTT CAGCGCTCTC AGAACCGTGC ATGGAAACAA GACGGATGTC
    AAGGATTATC TGATAGGAGA ATATTCTATG GGGAAGTCTC AGGCTCCAGT GTTTGATGTG
    TTTGAAGCCT TCATTAACAC AGACAACATT CAGGTGTTTG CTAACGCCGC CACCGACTAC
    ATCATGTGCC TTATGAAGTT TGTCAAAGGT TTAGGTGAAG TGGACTATAA GGAGATTGGC
    GATTGTGTTC ATGTCAGTGG ATACAGCTCT ACAGATCTGT GTCTGCCTGC GCTCGATTAC
    CTGAGGAAGT GCTCACAGCT GCTTGCCAAG ATCTATAAGA TGCCATCAAA GCCTGTGTTT
    CTGGGTGCGC GGCTGGCGAG TCTCCCAATG AGAGTGCAGG AGAGATCCGT GAGCAGCGAA
    GACGGCTTGG ACTGTGTGCT AGCCGAGTTT GATGACGATA CAGGTCTGAT TCAGGTGTGG
    ATCCTGCTGT TGGAGCAGCT GACAGCCGCT GTGTCCAACT GTCCCCGACA GCATCAGCCG
    CCGACCCTCG AGCTGCTGTT TGAGCTTCTG CGGGAGGTCA CCAAAATACC AGGGCCCGGT
    TTCGCCATCT TCTCTGTCAT CCAGCTGCTT CTTCCTGTGA TGTCACTGTG GCTCCAGCGT
    AGTCATGGCG ATCACTCATA CTGGGACATC GCAGCCGCAA ACTTCAAACA CGCCATCGGA
    CTGTCCTGTG AGCTGGTGGT GGAGCACATC CACAGCTTCA TACATTCAGA TATTGGCTAT
    GAGAGTCTGA TTAACCTGAT GCTGAAAGAT CTCTTCAAAC TGCTGGTTTC CTGTGTGGCA
    GAACCGGCTG AAACCATTTC CAGAGTCGGC TGCTCCTGTA TCAGGTATGT TCTGGTAACC
    GCCGGGCCTG TTTTCACTGA GGAGATGTGG CGTTTAGCAT GCTGTGCCCT GCAGGACGCT
    TTCTCAGCCA CTTTGGAGCC TGTCAAGAAC TTGCTGGCCT GTTTCCGGAG CGGTTCAGAC
    AGTTTCACTG GAGATGCGTG TGAAGTGAAA GTGGCAGCCC CGTCACACTC TCCCTCAGCA
    GAAGCAGAGA ACATGAGGAT TCGGGCCATG GCTCAACAGG TGTTCATGCT GGATACGCAG
    TGCTCTCCTA AAACTCCCAG CAGCAAGGAT GGGTTTGAGC ATGCTCAGTC CTGCGTGCTG
    ATCATTGAGC TTCCAGCTGA CCAGCACTCC AACGGCCAAG CTCAGAAGAG GATCCCGTTC
    AGGACTCTGG TTGTGAGTTT ACTGTCCCAT CAGGTGCTCC TGCAGAATCT GTATGACATC
    CTGTTAGAGG AGTTTGTCAA AGGTTCTGAG GGCAAAATCA CGGCTGTGTC GGAGCCAAAA
    CCTGCAGGAT TTCTCCGCTA CATCTCCATG CAGAACCTGG CCATCATCTT TGACCTCCTG
    CTGGACTCGT ATCGGACTGC CCGGGAATTT GACACCCGTC CTGGCCTGAA GTACCTGCTC
    ATGAAAGTGT CCGGAGTGTG TGGAGCTGCT AACTTATACC GCCAGTCTGC CATGAGCTTC
    AATATCTACT TTCAGGCTCT GCTTTGTGCA ATGCTGACAA ACCAGGAGAA CATGAGCGTG
    GAGCAGGTGA AGAAGATCCT GAGTGAGGAA GAGGAGGGCG GATCAGACTC CTCTCAGCAG
    TGCTCCTCCG AGGACGAGGA TATATTTGAA GAAATGGCTC AAGTGAGTCC TCCACGTGCC
    CGAGAGAAAC GTCAGTGGCA CACGCGTATC CCATCTCTGA GCACGCAGCC CGCCAGCAGT
    GCCGACTGGG CCTGGCTGGT AAAACGTCTG CACAAACTCT GCATGGACTT GTGCAACAAC
    TACATCCAGA TGCACCTGGA CCTGGAGAGT ACTTTGGGAG AGCAACCCAC CCATCGTGCT
    GACCCACTCT TTTTCTTGCC CCTTTTTAAT TCTGAGACTT CCACGCCCTC AACTGGTGGC
    CTGTCTGGCC ACGGGACTCC TTCAGAAGAC AGTTTCCGCC TTAACCTGGA TGAGACGCCC
    TCAGAAGACG TTCATAGTCC CGGCGGGTCA AACGCAAGCT TTCCCCAGCG GCGGGACGGG
    GGCCGCAAGA AGGAATGGTG GGAGAGTGCT GGAAACAAGC TGTACACCAT AGCCACCGAC
    AAGACCATTA CCAAACTGAT GATCGAGTAC AAGAAACGCA AGCAGCAGCA GCAGCACAAT
    CACACCATGT TTGCCAAAGA TGTGAAGGCA GGAGAAAAGA GGGGCGAGCC TGTGACCCTA
    CGAGGCCCGG ACTCGCCCGG ACCGCAGAGG CCCCAACAGC TGGTTGAACA GGGGCCCATG
    AGGCATTCCT TTAGTGCGGG ACCCGAGCTG CTGCGGCAGG AGAAAAGACC ACGATCGGGA
    TCCACAGCCA GTTCACACAA CATCTCTCTC AGAGATTCTG AGGCGCAGAT ACAGGCCTGG
    ACAAACATGG TCCTGACCAT CCTGAATCAG ATCCAGCTTC TGCCGGACCC CACGTTCATG
    GCTCTGCAGC CGGCGGTGTT TCCCTGCATC AGTCAGCTGA CCTGTCATGT GACCGAGCTG
    CGCGTGCGGC AGGCGGTGCG TGAGTGGCTG GGCCGAGTGG GACGCGTTTA TGACATCATC
    CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.