RAF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAF1
Entrez Gene ID	521196
Full Name	Raf-1 proto-oncogene, serine/threonine kinase
Synonyms	C-RAF
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024982727.1	XP_024838495.1	RAF proto-oncogene serine/threonine-protein kinase isoform X2
XM_024982726.1	XP_024838494.1	RAF proto-oncogene serine/threonine-protein kinase isoform X1
XM_024982729.1	XP_024838497.1	RAF proto-oncogene serine/threonine-protein kinase isoform X2
XM_024982728.1	XP_024838496.1	RAF proto-oncogene serine/threonine-protein kinase isoform X2
NM_001102505.1	NP_001095975.1	RAF proto-oncogene serine/threonine-protein kinase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Angiotensin II type 2 receptor-dependent increases in nitric oxide synthase expression in the pulmonary endothelium is mediated via a G alpha i3/Ras/Raf/MAPK pathway.
Li J, Zhao X, Li X, Lerea KM, Olson SC
American journal of physiology. Cell physiology292(6)C2185-96(2007 Jun)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)

Mapping HSA 3 loci in cattle: additional support for the ancestral synteny of HSA 3 and 21.
Threadgill DS, Womack JE
Genomics11(4)1143-8(1991 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data suggest that G alpha(i3), Shc, Grb2, Ras, and Raf-1 link Src to activation of MAPK and to the AT(2)-dependent increase in eNOS expression in PAECs
Title: Angiotensin II type 2 receptor-dependent increases in nitric oxide synthase expression in the pulmonary endothelium is mediated via a G alpha i3/Ras/Raf/MAPK pathway.

The following RAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44192	NM_001102505.1 Latest version!	Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa44192	XM_024982726.1 Latest version!	Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa50369	XM_024982729.1 Latest version!	Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa50369	XM_024982728.1 Latest version!	Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa50369	XM_024982727.1 Latest version!	Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAF1 ( NM_001102505.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095975.1 Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), mRNA.

Price & Availability↑

CloneID	OBa44192
Clone ID Related Accession (Same CDS sequence)	XM_024982726.1 , NM_001102505.1
Accession Version	NM_001102505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	RAF proto-oncogene serine/threonine-protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151319.1. On Aug 29, 2007 this sequence version replaced XR_027356.1. ##Evidence-Data-START## Transcript exon combination :: BC151319.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145418 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCACA TACAGGGCGC CTGGAAGACG ATCAGCAACG GTTTCGGATT CAAAGACACG 
GTGTTTGACG GCACCAGCTG CATCTCCCCT ACAATAGTTC AGCAATTTGG CTATCAGCGT 
CGGGCGTCAG ATGATGGCAA ACTTACGGAC CCTTCTAAGA CAAGCAACAC TATCCGTGTG 
TTTTTGCCAA ACAAGCAAAG AACAGTGGTC AATGTGCGGA ATGGAATGAG CTTGCATGAC 
TGCCTCATGA AAGCTCTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTCCACG AACACAAGGG TAAAAAAGCA CGGTTAGATT GGAATACTGA TGCTGCCTCG 
TTGATCGGAG AGGAACTTCA AGTGGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC 
TTTGCTCGGA AAACCTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA GTTCTTGCTA 
AATGGATTTA GATGTCAGAC TTGTGGCTAC AAGTTTCACG AGCATTGTAG CACCAAAGTA 
CCCACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TATTGTTCCC AAATTCCACT 
GTTGGTGATG GCGGGGTCCC AGCGCTGCCT TCTTTGACGA TGCGTCGGAT GCGAGAGTCT 
GTTTCCCGGA TACCTCCTGG TTCCCAGCAC AGATATTCCA CACCCCATGC TTTCACATTC 
AGCGCCTCCA GCCCCTCCTC TGAAGGCTCC CTCTCCCAGA GGCAGAGGTC GACGTCCACG 
CCCAACGTGC ACATGGTCAG CGCCACCCTG CCTGTGGACA GCAGGATGAT CGAGAATAAC 
AGCCTGAATG CTTCTCCCAG GGCGTGGTCC AGACGATTTT GTGTGAGGGG AAGAGATGCA 
ATTCGAAGTC ACAGTGAATC AGGCTCCCCT TCAGCCTTGT CCAGCAGTCC CAACAATTTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGCGCCTG CACAGAGAGA GCGGGCGCCA 
GGCTCCAGCA CCCAGGAGAA GAACAAAATT AGGCCTCGTG GACAGAGAGA TTCGAGCTAT 
TACTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGATC AGGCTCCTTT 
GGGACTGTTT ATAAGGGCAA GTGGCATGGA GACGTTGCAG TAAAGATTCT GAAGGTCGTT 
GACCCCACCC CAGAGCAGTT CCAGGCCTTC AGGAATGAGG TGGCTGTCCT CCGCAAAACC 
CGGCACGTGA ACATCCTGCT CTTCATGGGG TACATGACGA AGGACAACCT GGCGATAGTG 
ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ACGTCCAGGA GACCAAGTTC 
CAGATGTTCC AGCTGATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAATAATA TATTTCTCCA TGAAGGCCTG 
ACGGTGAAAA TCGGTGATTT TGGATTGGCC ACGGTGAAGT CACGCTGGAG TGGTTCTCAG 
CAGGTCGAGC AGCCCACCGG CTCCATCCTG TGGATGGCCC CAGAGGTGAT CCGGATGCAG 
GATAATAACC CGTTCAGCTT CCAGTCCGAC GTCTACTCCT ACGGCATCGT GCTGTATGAG 
CTCATGACGG GGGAGCTCCC TTACTCCCAC ATCAACAACC GCGACCAGAT CATCTTCATG 
GTGGGCCGCG GGTATGCCTC CCCAGACCTC AGCAAGCTAT ACAAGAACTG CCCCAAAGCC 
ATGAAGAGGC TGGTAGCTGA CTGCGTGAAG AAAGTGAAGG AAGAAAGGCC TCTTTTTCCC 
CAGATCCTGT CTTCCATCGA GCTGCTCCAG CATTCTCTGC CCAAGATCAA CCGGAGTGCC 
TCGGAGCCCT CCCTGCACCG GGCGGCCCAC ACCGAGGACA TCAACGCCTG CACGCTGACC 
ACGTCCCCTC GGCTGCCTGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095975.1
CDS	363..2369
Translation	MEHIQGAWKTISNGFGFKDTVFDGTSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTVGDGGVPALPSLTMRRMRESVSRIPPGSQHRYSTPHAFTFSASSPSSEGSLSQRQRSTSTPNVHMVSATLPVDSRMIENNSLNASPRAWSRRFCVRGRDAIRSHSESGSPSALSSSPNNLSPTGWSQPKTPAPAQRERAPGSSTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSILWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF

Target ORF information:

RefSeq Version	NM_001102505.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102505.1

ORF Insert Sequence:

ATGGAGCACA TACAGGGCGC CTGGAAGACG ATCAGCAACG GTTTCGGATT CAAAGACACG 
GTGTTTGACG GCACCAGCTG CATCTCCCCT ACAATAGTTC AGCAATTTGG CTATCAGCGT 
CGGGCGTCAG ATGATGGCAA ACTTACGGAC CCTTCTAAGA CAAGCAACAC TATCCGTGTG 
TTTTTGCCAA ACAAGCAAAG AACAGTGGTC AATGTGCGGA ATGGAATGAG CTTGCATGAC 
TGCCTCATGA AAGCTCTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTCCACG AACACAAGGG TAAAAAAGCA CGGTTAGATT GGAATACTGA TGCTGCCTCG 
TTGATCGGAG AGGAACTTCA AGTGGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC 
TTTGCTCGGA AAACCTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA GTTCTTGCTA 
AATGGATTTA GATGTCAGAC TTGTGGCTAC AAGTTTCACG AGCATTGTAG CACCAAAGTA 
CCCACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TATTGTTCCC AAATTCCACT 
GTTGGTGATG GCGGGGTCCC AGCGCTGCCT TCTTTGACGA TGCGTCGGAT GCGAGAGTCT 
GTTTCCCGGA TACCTCCTGG TTCCCAGCAC AGATATTCCA CACCCCATGC TTTCACATTC 
AGCGCCTCCA GCCCCTCCTC TGAAGGCTCC CTCTCCCAGA GGCAGAGGTC GACGTCCACG 
CCCAACGTGC ACATGGTCAG CGCCACCCTG CCTGTGGACA GCAGGATGAT CGAGAATAAC 
AGCCTGAATG CTTCTCCCAG GGCGTGGTCC AGACGATTTT GTGTGAGGGG AAGAGATGCA 
ATTCGAAGTC ACAGTGAATC AGGCTCCCCT TCAGCCTTGT CCAGCAGTCC CAACAATTTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGCGCCTG CACAGAGAGA GCGGGCGCCA 
GGCTCCAGCA CCCAGGAGAA GAACAAAATT AGGCCTCGTG GACAGAGAGA TTCGAGCTAT 
TACTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGATC AGGCTCCTTT 
GGGACTGTTT ATAAGGGCAA GTGGCATGGA GACGTTGCAG TAAAGATTCT GAAGGTCGTT 
GACCCCACCC CAGAGCAGTT CCAGGCCTTC AGGAATGAGG TGGCTGTCCT CCGCAAAACC 
CGGCACGTGA ACATCCTGCT CTTCATGGGG TACATGACGA AGGACAACCT GGCGATAGTG 
ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ACGTCCAGGA GACCAAGTTC 
CAGATGTTCC AGCTGATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAATAATA TATTTCTCCA TGAAGGCCTG 
ACGGTGAAAA TCGGTGATTT TGGATTGGCC ACGGTGAAGT CACGCTGGAG TGGTTCTCAG 
CAGGTCGAGC AGCCCACCGG CTCCATCCTG TGGATGGCCC CAGAGGTGAT CCGGATGCAG 
GATAATAACC CGTTCAGCTT CCAGTCCGAC GTCTACTCCT ACGGCATCGT GCTGTATGAG 
CTCATGACGG GGGAGCTCCC TTACTCCCAC ATCAACAACC GCGACCAGAT CATCTTCATG 
GTGGGCCGCG GGTATGCCTC CCCAGACCTC AGCAAGCTAT ACAAGAACTG CCCCAAAGCC 
ATGAAGAGGC TGGTAGCTGA CTGCGTGAAG AAAGTGAAGG AAGAAAGGCC TCTTTTTCCC 
CAGATCCTGT CTTCCATCGA GCTGCTCCAG CATTCTCTGC CCAAGATCAA CCGGAGTGCC 
TCGGAGCCCT CCCTGCACCG GGCGGCCCAC ACCGAGGACA TCAACGCCTG CACGCTGACC 
ACGTCCCCTC GGCTGCCTGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAF1 ( XM_024982726.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838494.1 Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa44192
Clone ID Related Accession (Same CDS sequence)	XM_024982726.1 , NM_001102505.1
Accession Version	XM_024982726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA TACAGGGCGC CTGGAAGACG ATCAGCAACG GTTTCGGATT CAAAGACACG 
GTGTTTGACG GCACCAGCTG CATCTCCCCT ACAATAGTTC AGCAATTTGG CTATCAGCGT 
CGGGCGTCAG ATGATGGCAA ACTTACGGAC CCTTCTAAGA CAAGCAACAC TATCCGTGTG 
TTTTTGCCAA ACAAGCAAAG AACAGTGGTC AATGTGCGGA ATGGAATGAG CTTGCATGAC 
TGCCTCATGA AAGCTCTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTCCACG AACACAAGGG TAAAAAAGCA CGGTTAGATT GGAATACTGA TGCTGCCTCG 
TTGATCGGAG AGGAACTTCA AGTGGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC 
TTTGCTCGGA AAACCTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA GTTCTTGCTA 
AATGGATTTA GATGTCAGAC TTGTGGCTAC AAGTTTCACG AGCATTGTAG CACCAAAGTA 
CCCACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TATTGTTCCC AAATTCCACT 
GTTGGTGATG GCGGGGTCCC AGCGCTGCCT TCTTTGACGA TGCGTCGGAT GCGAGAGTCT 
GTTTCCCGGA TACCTCCTGG TTCCCAGCAC AGATATTCCA CACCCCATGC TTTCACATTC 
AGCGCCTCCA GCCCCTCCTC TGAAGGCTCC CTCTCCCAGA GGCAGAGGTC GACGTCCACG 
CCCAACGTGC ACATGGTCAG CGCCACCCTG CCTGTGGACA GCAGGATGAT CGAGAATAAC 
AGCCTGAATG CTTCTCCCAG GGCGTGGTCC AGACGATTTT GTGTGAGGGG AAGAGATGCA 
ATTCGAAGTC ACAGTGAATC AGGCTCCCCT TCAGCCTTGT CCAGCAGTCC CAACAATTTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGCGCCTG CACAGAGAGA GCGGGCGCCA 
GGCTCCAGCA CCCAGGAGAA GAACAAAATT AGGCCTCGTG GACAGAGAGA TTCGAGCTAT 
TACTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGATC AGGCTCCTTT 
GGGACTGTTT ATAAGGGCAA GTGGCATGGA GACGTTGCAG TAAAGATTCT GAAGGTCGTT 
GACCCCACCC CAGAGCAGTT CCAGGCCTTC AGGAATGAGG TGGCTGTCCT CCGCAAAACC 
CGGCACGTGA ACATCCTGCT CTTCATGGGG TACATGACGA AGGACAACCT GGCGATAGTG 
ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ACGTCCAGGA GACCAAGTTC 
CAGATGTTCC AGCTGATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAATAATA TATTTCTCCA TGAAGGCCTG 
ACGGTGAAAA TCGGTGATTT TGGATTGGCC ACGGTGAAGT CACGCTGGAG TGGTTCTCAG 
CAGGTCGAGC AGCCCACCGG CTCCATCCTG TGGATGGCCC CAGAGGTGAT CCGGATGCAG 
GATAATAACC CGTTCAGCTT CCAGTCCGAC GTCTACTCCT ACGGCATCGT GCTGTATGAG 
CTCATGACGG GGGAGCTCCC TTACTCCCAC ATCAACAACC GCGACCAGAT CATCTTCATG 
GTGGGCCGCG GGTATGCCTC CCCAGACCTC AGCAAGCTAT ACAAGAACTG CCCCAAAGCC 
ATGAAGAGGC TGGTAGCTGA CTGCGTGAAG AAAGTGAAGG AAGAAAGGCC TCTTTTTCCC 
CAGATCCTGT CTTCCATCGA GCTGCTCCAG CATTCTCTGC CCAAGATCAA CCGGAGTGCC 
TCGGAGCCCT CCCTGCACCG GGCGGCCCAC ACCGAGGACA TCAACGCCTG CACGCTGACC 
ACGTCCCCTC GGCTGCCTGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838494.1
CDS	526..2532
Translation	MEHIQGAWKTISNGFGFKDTVFDGTSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTVGDGGVPALPSLTMRRMRESVSRIPPGSQHRYSTPHAFTFSASSPSSEGSLSQRQRSTSTPNVHMVSATLPVDSRMIENNSLNASPRAWSRRFCVRGRDAIRSHSESGSPSALSSSPNNLSPTGWSQPKTPAPAQRERAPGSSTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSILWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF

Target ORF information:

RefSeq Version	XM_024982726.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982726.1

ORF Insert Sequence:

ATGGAGCACA TACAGGGCGC CTGGAAGACG ATCAGCAACG GTTTCGGATT CAAAGACACG 
GTGTTTGACG GCACCAGCTG CATCTCCCCT ACAATAGTTC AGCAATTTGG CTATCAGCGT 
CGGGCGTCAG ATGATGGCAA ACTTACGGAC CCTTCTAAGA CAAGCAACAC TATCCGTGTG 
TTTTTGCCAA ACAAGCAAAG AACAGTGGTC AATGTGCGGA ATGGAATGAG CTTGCATGAC 
TGCCTCATGA AAGCTCTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTCCACG AACACAAGGG TAAAAAAGCA CGGTTAGATT GGAATACTGA TGCTGCCTCG 
TTGATCGGAG AGGAACTTCA AGTGGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC 
TTTGCTCGGA AAACCTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA GTTCTTGCTA 
AATGGATTTA GATGTCAGAC TTGTGGCTAC AAGTTTCACG AGCATTGTAG CACCAAAGTA 
CCCACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TATTGTTCCC AAATTCCACT 
GTTGGTGATG GCGGGGTCCC AGCGCTGCCT TCTTTGACGA TGCGTCGGAT GCGAGAGTCT 
GTTTCCCGGA TACCTCCTGG TTCCCAGCAC AGATATTCCA CACCCCATGC TTTCACATTC 
AGCGCCTCCA GCCCCTCCTC TGAAGGCTCC CTCTCCCAGA GGCAGAGGTC GACGTCCACG 
CCCAACGTGC ACATGGTCAG CGCCACCCTG CCTGTGGACA GCAGGATGAT CGAGAATAAC 
AGCCTGAATG CTTCTCCCAG GGCGTGGTCC AGACGATTTT GTGTGAGGGG AAGAGATGCA 
ATTCGAAGTC ACAGTGAATC AGGCTCCCCT TCAGCCTTGT CCAGCAGTCC CAACAATTTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGCGCCTG CACAGAGAGA GCGGGCGCCA 
GGCTCCAGCA CCCAGGAGAA GAACAAAATT AGGCCTCGTG GACAGAGAGA TTCGAGCTAT 
TACTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGATC AGGCTCCTTT 
GGGACTGTTT ATAAGGGCAA GTGGCATGGA GACGTTGCAG TAAAGATTCT GAAGGTCGTT 
GACCCCACCC CAGAGCAGTT CCAGGCCTTC AGGAATGAGG TGGCTGTCCT CCGCAAAACC 
CGGCACGTGA ACATCCTGCT CTTCATGGGG TACATGACGA AGGACAACCT GGCGATAGTG 
ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ACGTCCAGGA GACCAAGTTC 
CAGATGTTCC AGCTGATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAATAATA TATTTCTCCA TGAAGGCCTG 
ACGGTGAAAA TCGGTGATTT TGGATTGGCC ACGGTGAAGT CACGCTGGAG TGGTTCTCAG 
CAGGTCGAGC AGCCCACCGG CTCCATCCTG TGGATGGCCC CAGAGGTGAT CCGGATGCAG 
GATAATAACC CGTTCAGCTT CCAGTCCGAC GTCTACTCCT ACGGCATCGT GCTGTATGAG 
CTCATGACGG GGGAGCTCCC TTACTCCCAC ATCAACAACC GCGACCAGAT CATCTTCATG 
GTGGGCCGCG GGTATGCCTC CCCAGACCTC AGCAAGCTAT ACAAGAACTG CCCCAAAGCC 
ATGAAGAGGC TGGTAGCTGA CTGCGTGAAG AAAGTGAAGG AAGAAAGGCC TCTTTTTCCC 
CAGATCCTGT CTTCCATCGA GCTGCTCCAG CATTCTCTGC CCAAGATCAA CCGGAGTGCC 
TCGGAGCCCT CCCTGCACCG GGCGGCCCAC ACCGAGGACA TCAACGCCTG CACGCTGACC 
ACGTCCCCTC GGCTGCCTGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAF1 ( XM_024982727.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838495.1 Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa50369
Clone ID Related Accession (Same CDS sequence)	XM_024982727.1 , XM_024982729.1 , XM_024982728.1
Accession Version	XM_024982727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA TACAGGGCGC CTGGAAGACG ATCAGCAACG GTTTCGGATT CAAAGACACG 
GTGTTTGACG GCACCAGCTG CATCTCCCCT ACAATAGTTC AGCAATTTGG CTATCAGCGT 
CGGGCGTCAG ATGATGGCAA ACTTACGGAC CCTTCTAAGA CAAGCAACAC TATCCGTGTG 
TTTTTGCCAA ACAAGCAAAG AACAGTGGTC AATGTGCGGA ATGGAATGAG CTTGCATGAC 
TGCCTCATGA AAGCTCTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTCCACG AACACAAGGG TAAAAAAGCA CGGTTAGATT GGAATACTGA TGCTGCCTCG 
TTGATCGGAG AGGAACTTCA AGTGGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC 
TTTGCTCGGA AAACCTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA GTTCTTGCTA 
AATGGATTTA GATGTCAGAC TTGTGGCTAC AAGTTTCACG AGCATTGTAG CACCAAAGTA 
CCCACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TATTGTTCCC AAATTCCACT 
GTTGGTGATG GCGGGGTCCC AGCGCTGCCT TCTTTGACGA TGCGTCGGAT GCGAGAGTCT 
GTTTCCCGGA TACCTCCTGG TTCCCAGCAC AGATATTCCA CACCCCATGC TTTCACATTC 
AGCGCCTCCA GCCCCTCCTC TGAAGGCTCC CTCTCCCAGA GGCAGAGGTC GACGTCCACG 
CCCAACGTGC ACATGGTCAG CGCCACCCTG CCTGTGGACA GCAGGATGAT CGAGGATGCA 
ATTCGAAGTC ACAGTGAATC AGGCTCCCCT TCAGCCTTGT CCAGCAGTCC CAACAATTTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGCGCCTG CACAGAGAGA GCGGGCGCCA 
GGCTCCAGCA CCCAGGAGAA GAACAAAATT AGGCCTCGTG GACAGAGAGA TTCGAGCTAT 
TACTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGATC AGGCTCCTTT 
GGGACTGTTT ATAAGGGCAA GTGGCATGGA GACGTTGCAG TAAAGATTCT GAAGGTCGTT 
GACCCCACCC CAGAGCAGTT CCAGGCCTTC AGGAATGAGG TGGCTGTCCT CCGCAAAACC 
CGGCACGTGA ACATCCTGCT CTTCATGGGG TACATGACGA AGGACAACCT GGCGATAGTG 
ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ACGTCCAGGA GACCAAGTTC 
CAGATGTTCC AGCTGATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAATAATA TATTTCTCCA TGAAGGCCTG 
ACGGTGAAAA TCGGTGATTT TGGATTGGCC ACGGTGAAGT CACGCTGGAG TGGTTCTCAG 
CAGGTCGAGC AGCCCACCGG CTCCATCCTG TGGATGGCCC CAGAGGTGAT CCGGATGCAG 
GATAATAACC CGTTCAGCTT CCAGTCCGAC GTCTACTCCT ACGGCATCGT GCTGTATGAG 
CTCATGACGG GGGAGCTCCC TTACTCCCAC ATCAACAACC GCGACCAGAT CATCTTCATG 
GTGGGCCGCG GGTATGCCTC CCCAGACCTC AGCAAGCTAT ACAAGAACTG CCCCAAAGCC 
ATGAAGAGGC TGGTAGCTGA CTGCGTGAAG AAAGTGAAGG AAGAAAGGCC TCTTTTTCCC 
CAGATCCTGT CTTCCATCGA GCTGCTCCAG CATTCTCTGC CCAAGATCAA CCGGAGTGCC 
TCGGAGCCCT CCCTGCACCG GGCGGCCCAC ACCGAGGACA TCAACGCCTG CACGCTGACC 
ACGTCCCCTC GGCTGCCTGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838495.1
CDS	368..2314
Translation	MEHIQGAWKTISNGFGFKDTVFDGTSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTVGDGGVPALPSLTMRRMRESVSRIPPGSQHRYSTPHAFTFSASSPSSEGSLSQRQRSTSTPNVHMVSATLPVDSRMIEDAIRSHSESGSPSALSSSPNNLSPTGWSQPKTPAPAQRERAPGSSTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSILWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF

Target ORF information:

RefSeq Version	XM_024982727.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982727.1

ORF Insert Sequence:

ATGGAGCACA TACAGGGCGC CTGGAAGACG ATCAGCAACG GTTTCGGATT CAAAGACACG 
GTGTTTGACG GCACCAGCTG CATCTCCCCT ACAATAGTTC AGCAATTTGG CTATCAGCGT 
CGGGCGTCAG ATGATGGCAA ACTTACGGAC CCTTCTAAGA CAAGCAACAC TATCCGTGTG 
TTTTTGCCAA ACAAGCAAAG AACAGTGGTC AATGTGCGGA ATGGAATGAG CTTGCATGAC 
TGCCTCATGA AAGCTCTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTCCACG AACACAAGGG TAAAAAAGCA CGGTTAGATT GGAATACTGA TGCTGCCTCG 
TTGATCGGAG AGGAACTTCA AGTGGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC 
TTTGCTCGGA AAACCTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA GTTCTTGCTA 
AATGGATTTA GATGTCAGAC TTGTGGCTAC AAGTTTCACG AGCATTGTAG CACCAAAGTA 
CCCACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TATTGTTCCC AAATTCCACT 
GTTGGTGATG GCGGGGTCCC AGCGCTGCCT TCTTTGACGA TGCGTCGGAT GCGAGAGTCT 
GTTTCCCGGA TACCTCCTGG TTCCCAGCAC AGATATTCCA CACCCCATGC TTTCACATTC 
AGCGCCTCCA GCCCCTCCTC TGAAGGCTCC CTCTCCCAGA GGCAGAGGTC GACGTCCACG 
CCCAACGTGC ACATGGTCAG CGCCACCCTG CCTGTGGACA GCAGGATGAT CGAGGATGCA 
ATTCGAAGTC ACAGTGAATC AGGCTCCCCT TCAGCCTTGT CCAGCAGTCC CAACAATTTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGCGCCTG CACAGAGAGA GCGGGCGCCA 
GGCTCCAGCA CCCAGGAGAA GAACAAAATT AGGCCTCGTG GACAGAGAGA TTCGAGCTAT 
TACTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGATC AGGCTCCTTT 
GGGACTGTTT ATAAGGGCAA GTGGCATGGA GACGTTGCAG TAAAGATTCT GAAGGTCGTT 
GACCCCACCC CAGAGCAGTT CCAGGCCTTC AGGAATGAGG TGGCTGTCCT CCGCAAAACC 
CGGCACGTGA ACATCCTGCT CTTCATGGGG TACATGACGA AGGACAACCT GGCGATAGTG 
ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ACGTCCAGGA GACCAAGTTC 
CAGATGTTCC AGCTGATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAATAATA TATTTCTCCA TGAAGGCCTG 
ACGGTGAAAA TCGGTGATTT TGGATTGGCC ACGGTGAAGT CACGCTGGAG TGGTTCTCAG 
CAGGTCGAGC AGCCCACCGG CTCCATCCTG TGGATGGCCC CAGAGGTGAT CCGGATGCAG 
GATAATAACC CGTTCAGCTT CCAGTCCGAC GTCTACTCCT ACGGCATCGT GCTGTATGAG 
CTCATGACGG GGGAGCTCCC TTACTCCCAC ATCAACAACC GCGACCAGAT CATCTTCATG 
GTGGGCCGCG GGTATGCCTC CCCAGACCTC AGCAAGCTAT ACAAGAACTG CCCCAAAGCC 
ATGAAGAGGC TGGTAGCTGA CTGCGTGAAG AAAGTGAAGG AAGAAAGGCC TCTTTTTCCC 
CAGATCCTGT CTTCCATCGA GCTGCTCCAG CATTCTCTGC CCAAGATCAA CCGGAGTGCC 
TCGGAGCCCT CCCTGCACCG GGCGGCCCAC ACCGAGGACA TCAACGCCTG CACGCTGACC 
ACGTCCCCTC GGCTGCCTGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAF1 ( XM_024982729.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838497.1 Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa50369
Clone ID Related Accession (Same CDS sequence)	XM_024982727.1 , XM_024982729.1 , XM_024982728.1
Accession Version	XM_024982729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA TACAGGGCGC CTGGAAGACG ATCAGCAACG GTTTCGGATT CAAAGACACG 
GTGTTTGACG GCACCAGCTG CATCTCCCCT ACAATAGTTC AGCAATTTGG CTATCAGCGT 
CGGGCGTCAG ATGATGGCAA ACTTACGGAC CCTTCTAAGA CAAGCAACAC TATCCGTGTG 
TTTTTGCCAA ACAAGCAAAG AACAGTGGTC AATGTGCGGA ATGGAATGAG CTTGCATGAC 
TGCCTCATGA AAGCTCTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTCCACG AACACAAGGG TAAAAAAGCA CGGTTAGATT GGAATACTGA TGCTGCCTCG 
TTGATCGGAG AGGAACTTCA AGTGGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC 
TTTGCTCGGA AAACCTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA GTTCTTGCTA 
AATGGATTTA GATGTCAGAC TTGTGGCTAC AAGTTTCACG AGCATTGTAG CACCAAAGTA 
CCCACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TATTGTTCCC AAATTCCACT 
GTTGGTGATG GCGGGGTCCC AGCGCTGCCT TCTTTGACGA TGCGTCGGAT GCGAGAGTCT 
GTTTCCCGGA TACCTCCTGG TTCCCAGCAC AGATATTCCA CACCCCATGC TTTCACATTC 
AGCGCCTCCA GCCCCTCCTC TGAAGGCTCC CTCTCCCAGA GGCAGAGGTC GACGTCCACG 
CCCAACGTGC ACATGGTCAG CGCCACCCTG CCTGTGGACA GCAGGATGAT CGAGGATGCA 
ATTCGAAGTC ACAGTGAATC AGGCTCCCCT TCAGCCTTGT CCAGCAGTCC CAACAATTTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGCGCCTG CACAGAGAGA GCGGGCGCCA 
GGCTCCAGCA CCCAGGAGAA GAACAAAATT AGGCCTCGTG GACAGAGAGA TTCGAGCTAT 
TACTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGATC AGGCTCCTTT 
GGGACTGTTT ATAAGGGCAA GTGGCATGGA GACGTTGCAG TAAAGATTCT GAAGGTCGTT 
GACCCCACCC CAGAGCAGTT CCAGGCCTTC AGGAATGAGG TGGCTGTCCT CCGCAAAACC 
CGGCACGTGA ACATCCTGCT CTTCATGGGG TACATGACGA AGGACAACCT GGCGATAGTG 
ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ACGTCCAGGA GACCAAGTTC 
CAGATGTTCC AGCTGATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAATAATA TATTTCTCCA TGAAGGCCTG 
ACGGTGAAAA TCGGTGATTT TGGATTGGCC ACGGTGAAGT CACGCTGGAG TGGTTCTCAG 
CAGGTCGAGC AGCCCACCGG CTCCATCCTG TGGATGGCCC CAGAGGTGAT CCGGATGCAG 
GATAATAACC CGTTCAGCTT CCAGTCCGAC GTCTACTCCT ACGGCATCGT GCTGTATGAG 
CTCATGACGG GGGAGCTCCC TTACTCCCAC ATCAACAACC GCGACCAGAT CATCTTCATG 
GTGGGCCGCG GGTATGCCTC CCCAGACCTC AGCAAGCTAT ACAAGAACTG CCCCAAAGCC 
ATGAAGAGGC TGGTAGCTGA CTGCGTGAAG AAAGTGAAGG AAGAAAGGCC TCTTTTTCCC 
CAGATCCTGT CTTCCATCGA GCTGCTCCAG CATTCTCTGC CCAAGATCAA CCGGAGTGCC 
TCGGAGCCCT CCCTGCACCG GGCGGCCCAC ACCGAGGACA TCAACGCCTG CACGCTGACC 
ACGTCCCCTC GGCTGCCTGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838497.1
CDS	163..2109
Translation	MEHIQGAWKTISNGFGFKDTVFDGTSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTVGDGGVPALPSLTMRRMRESVSRIPPGSQHRYSTPHAFTFSASSPSSEGSLSQRQRSTSTPNVHMVSATLPVDSRMIEDAIRSHSESGSPSALSSSPNNLSPTGWSQPKTPAPAQRERAPGSSTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSILWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF

Target ORF information:

RefSeq Version	XM_024982729.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982729.1

ORF Insert Sequence:

ATGGAGCACA TACAGGGCGC CTGGAAGACG ATCAGCAACG GTTTCGGATT CAAAGACACG 
GTGTTTGACG GCACCAGCTG CATCTCCCCT ACAATAGTTC AGCAATTTGG CTATCAGCGT 
CGGGCGTCAG ATGATGGCAA ACTTACGGAC CCTTCTAAGA CAAGCAACAC TATCCGTGTG 
TTTTTGCCAA ACAAGCAAAG AACAGTGGTC AATGTGCGGA ATGGAATGAG CTTGCATGAC 
TGCCTCATGA AAGCTCTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTCCACG AACACAAGGG TAAAAAAGCA CGGTTAGATT GGAATACTGA TGCTGCCTCG 
TTGATCGGAG AGGAACTTCA AGTGGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC 
TTTGCTCGGA AAACCTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA GTTCTTGCTA 
AATGGATTTA GATGTCAGAC TTGTGGCTAC AAGTTTCACG AGCATTGTAG CACCAAAGTA 
CCCACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TATTGTTCCC AAATTCCACT 
GTTGGTGATG GCGGGGTCCC AGCGCTGCCT TCTTTGACGA TGCGTCGGAT GCGAGAGTCT 
GTTTCCCGGA TACCTCCTGG TTCCCAGCAC AGATATTCCA CACCCCATGC TTTCACATTC 
AGCGCCTCCA GCCCCTCCTC TGAAGGCTCC CTCTCCCAGA GGCAGAGGTC GACGTCCACG 
CCCAACGTGC ACATGGTCAG CGCCACCCTG CCTGTGGACA GCAGGATGAT CGAGGATGCA 
ATTCGAAGTC ACAGTGAATC AGGCTCCCCT TCAGCCTTGT CCAGCAGTCC CAACAATTTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGCGCCTG CACAGAGAGA GCGGGCGCCA 
GGCTCCAGCA CCCAGGAGAA GAACAAAATT AGGCCTCGTG GACAGAGAGA TTCGAGCTAT 
TACTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGATC AGGCTCCTTT 
GGGACTGTTT ATAAGGGCAA GTGGCATGGA GACGTTGCAG TAAAGATTCT GAAGGTCGTT 
GACCCCACCC CAGAGCAGTT CCAGGCCTTC AGGAATGAGG TGGCTGTCCT CCGCAAAACC 
CGGCACGTGA ACATCCTGCT CTTCATGGGG TACATGACGA AGGACAACCT GGCGATAGTG 
ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ACGTCCAGGA GACCAAGTTC 
CAGATGTTCC AGCTGATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAATAATA TATTTCTCCA TGAAGGCCTG 
ACGGTGAAAA TCGGTGATTT TGGATTGGCC ACGGTGAAGT CACGCTGGAG TGGTTCTCAG 
CAGGTCGAGC AGCCCACCGG CTCCATCCTG TGGATGGCCC CAGAGGTGAT CCGGATGCAG 
GATAATAACC CGTTCAGCTT CCAGTCCGAC GTCTACTCCT ACGGCATCGT GCTGTATGAG 
CTCATGACGG GGGAGCTCCC TTACTCCCAC ATCAACAACC GCGACCAGAT CATCTTCATG 
GTGGGCCGCG GGTATGCCTC CCCAGACCTC AGCAAGCTAT ACAAGAACTG CCCCAAAGCC 
ATGAAGAGGC TGGTAGCTGA CTGCGTGAAG AAAGTGAAGG AAGAAAGGCC TCTTTTTCCC 
CAGATCCTGT CTTCCATCGA GCTGCTCCAG CATTCTCTGC CCAAGATCAA CCGGAGTGCC 
TCGGAGCCCT CCCTGCACCG GGCGGCCCAC ACCGAGGACA TCAACGCCTG CACGCTGACC 
ACGTCCCCTC GGCTGCCTGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAF1 ( XM_024982728.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838496.1 Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa50369
Clone ID Related Accession (Same CDS sequence)	XM_024982727.1 , XM_024982729.1 , XM_024982728.1
Accession Version	XM_024982728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA TACAGGGCGC CTGGAAGACG ATCAGCAACG GTTTCGGATT CAAAGACACG 
GTGTTTGACG GCACCAGCTG CATCTCCCCT ACAATAGTTC AGCAATTTGG CTATCAGCGT 
CGGGCGTCAG ATGATGGCAA ACTTACGGAC CCTTCTAAGA CAAGCAACAC TATCCGTGTG 
TTTTTGCCAA ACAAGCAAAG AACAGTGGTC AATGTGCGGA ATGGAATGAG CTTGCATGAC 
TGCCTCATGA AAGCTCTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTCCACG AACACAAGGG TAAAAAAGCA CGGTTAGATT GGAATACTGA TGCTGCCTCG 
TTGATCGGAG AGGAACTTCA AGTGGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC 
TTTGCTCGGA AAACCTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA GTTCTTGCTA 
AATGGATTTA GATGTCAGAC TTGTGGCTAC AAGTTTCACG AGCATTGTAG CACCAAAGTA 
CCCACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TATTGTTCCC AAATTCCACT 
GTTGGTGATG GCGGGGTCCC AGCGCTGCCT TCTTTGACGA TGCGTCGGAT GCGAGAGTCT 
GTTTCCCGGA TACCTCCTGG TTCCCAGCAC AGATATTCCA CACCCCATGC TTTCACATTC 
AGCGCCTCCA GCCCCTCCTC TGAAGGCTCC CTCTCCCAGA GGCAGAGGTC GACGTCCACG 
CCCAACGTGC ACATGGTCAG CGCCACCCTG CCTGTGGACA GCAGGATGAT CGAGGATGCA 
ATTCGAAGTC ACAGTGAATC AGGCTCCCCT TCAGCCTTGT CCAGCAGTCC CAACAATTTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGCGCCTG CACAGAGAGA GCGGGCGCCA 
GGCTCCAGCA CCCAGGAGAA GAACAAAATT AGGCCTCGTG GACAGAGAGA TTCGAGCTAT 
TACTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGATC AGGCTCCTTT 
GGGACTGTTT ATAAGGGCAA GTGGCATGGA GACGTTGCAG TAAAGATTCT GAAGGTCGTT 
GACCCCACCC CAGAGCAGTT CCAGGCCTTC AGGAATGAGG TGGCTGTCCT CCGCAAAACC 
CGGCACGTGA ACATCCTGCT CTTCATGGGG TACATGACGA AGGACAACCT GGCGATAGTG 
ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ACGTCCAGGA GACCAAGTTC 
CAGATGTTCC AGCTGATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAATAATA TATTTCTCCA TGAAGGCCTG 
ACGGTGAAAA TCGGTGATTT TGGATTGGCC ACGGTGAAGT CACGCTGGAG TGGTTCTCAG 
CAGGTCGAGC AGCCCACCGG CTCCATCCTG TGGATGGCCC CAGAGGTGAT CCGGATGCAG 
GATAATAACC CGTTCAGCTT CCAGTCCGAC GTCTACTCCT ACGGCATCGT GCTGTATGAG 
CTCATGACGG GGGAGCTCCC TTACTCCCAC ATCAACAACC GCGACCAGAT CATCTTCATG 
GTGGGCCGCG GGTATGCCTC CCCAGACCTC AGCAAGCTAT ACAAGAACTG CCCCAAAGCC 
ATGAAGAGGC TGGTAGCTGA CTGCGTGAAG AAAGTGAAGG AAGAAAGGCC TCTTTTTCCC 
CAGATCCTGT CTTCCATCGA GCTGCTCCAG CATTCTCTGC CCAAGATCAA CCGGAGTGCC 
TCGGAGCCCT CCCTGCACCG GGCGGCCCAC ACCGAGGACA TCAACGCCTG CACGCTGACC 
ACGTCCCCTC GGCTGCCTGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838496.1
CDS	484..2430
Translation	MEHIQGAWKTISNGFGFKDTVFDGTSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTVGDGGVPALPSLTMRRMRESVSRIPPGSQHRYSTPHAFTFSASSPSSEGSLSQRQRSTSTPNVHMVSATLPVDSRMIEDAIRSHSESGSPSALSSSPNNLSPTGWSQPKTPAPAQRERAPGSSTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSILWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF

Target ORF information:

RefSeq Version	XM_024982728.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Raf-1 proto-oncogene, serine/threonine kinase (RAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982728.1

ORF Insert Sequence:

ATGGAGCACA TACAGGGCGC CTGGAAGACG ATCAGCAACG GTTTCGGATT CAAAGACACG 
GTGTTTGACG GCACCAGCTG CATCTCCCCT ACAATAGTTC AGCAATTTGG CTATCAGCGT 
CGGGCGTCAG ATGATGGCAA ACTTACGGAC CCTTCTAAGA CAAGCAACAC TATCCGTGTG 
TTTTTGCCAA ACAAGCAAAG AACAGTGGTC AATGTGCGGA ATGGAATGAG CTTGCATGAC 
TGCCTCATGA AAGCTCTCAA GGTGAGGGGC CTGCAACCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTCCACG AACACAAGGG TAAAAAAGCA CGGTTAGATT GGAATACTGA TGCTGCCTCG 
TTGATCGGAG AGGAACTTCA AGTGGATTTC CTGGATCATG TTCCCCTCAC AACACACAAC 
TTTGCTCGGA AAACCTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA GTTCTTGCTA 
AATGGATTTA GATGTCAGAC TTGTGGCTAC AAGTTTCACG AGCATTGTAG CACCAAAGTA 
CCCACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TATTGTTCCC AAATTCCACT 
GTTGGTGATG GCGGGGTCCC AGCGCTGCCT TCTTTGACGA TGCGTCGGAT GCGAGAGTCT 
GTTTCCCGGA TACCTCCTGG TTCCCAGCAC AGATATTCCA CACCCCATGC TTTCACATTC 
AGCGCCTCCA GCCCCTCCTC TGAAGGCTCC CTCTCCCAGA GGCAGAGGTC GACGTCCACG 
CCCAACGTGC ACATGGTCAG CGCCACCCTG CCTGTGGACA GCAGGATGAT CGAGGATGCA 
ATTCGAAGTC ACAGTGAATC AGGCTCCCCT TCAGCCTTGT CCAGCAGTCC CAACAATTTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGCGCCTG CACAGAGAGA GCGGGCGCCA 
GGCTCCAGCA CCCAGGAGAA GAACAAAATT AGGCCTCGTG GACAGAGAGA TTCGAGCTAT 
TACTGGGAAA TAGAAGCCAG TGAAGTGATG CTGTCCACTC GGATTGGATC AGGCTCCTTT 
GGGACTGTTT ATAAGGGCAA GTGGCATGGA GACGTTGCAG TAAAGATTCT GAAGGTCGTT 
GACCCCACCC CAGAGCAGTT CCAGGCCTTC AGGAATGAGG TGGCTGTCCT CCGCAAAACC 
CGGCACGTGA ACATCCTGCT CTTCATGGGG TACATGACGA AGGACAACCT GGCGATAGTG 
ACCCAGTGGT GCGAGGGCAG CAGCCTCTAC AAACACCTGC ACGTCCAGGA GACCAAGTTC 
CAGATGTTCC AGCTGATTGA CATTGCCCGG CAGACGGCTC AGGGAATGGA CTATTTGCAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAATAATA TATTTCTCCA TGAAGGCCTG 
ACGGTGAAAA TCGGTGATTT TGGATTGGCC ACGGTGAAGT CACGCTGGAG TGGTTCTCAG 
CAGGTCGAGC AGCCCACCGG CTCCATCCTG TGGATGGCCC CAGAGGTGAT CCGGATGCAG 
GATAATAACC CGTTCAGCTT CCAGTCCGAC GTCTACTCCT ACGGCATCGT GCTGTATGAG 
CTCATGACGG GGGAGCTCCC TTACTCCCAC ATCAACAACC GCGACCAGAT CATCTTCATG 
GTGGGCCGCG GGTATGCCTC CCCAGACCTC AGCAAGCTAT ACAAGAACTG CCCCAAAGCC 
ATGAAGAGGC TGGTAGCTGA CTGCGTGAAG AAAGTGAAGG AAGAAAGGCC TCTTTTTCCC 
CAGATCCTGT CTTCCATCGA GCTGCTCCAG CATTCTCTGC CCAAGATCAA CCGGAGTGCC 
TCGGAGCCCT CCCTGCACCG GGCGGCCCAC ACCGAGGACA TCAACGCCTG CACGCTGACC 
ACGTCCCCTC GGCTGCCTGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Angiotensin II type 2 receptor-dependent increases in nitric oxide synthase expression in the pulmonary endothelium is mediated via a G alpha i3/Ras/Raf/MAPK pathway.
American journal of physiology. Cell physiology292(6)C2185-96(2007 Jun)
Li J,Zhao X,Li X,Lerea KM,Olson SC

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)
Sonstegard TS,Capuco AV,White J,Van Tassell CP,Connor EE,Cho J,Sultana R,Shade L,Wray JE,Wells KD,Quackenbush J

Mapping HSA 3 loci in cattle: additional support for the ancestral synteny of HSA 3 and 21.
Genomics11(4)1143-8(1991 Dec)
Threadgill DS,Womack JE

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RAF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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