TRIM28 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIM28
Entrez Gene ID	519422
Full Name	tripartite motif containing 28
General protein information	Preferred Names tripartite motif containing 28 Names transcription intermediary factor 1-beta
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024978252.1	XP_024834020.1	transcription intermediary factor 1-beta isoform X2
XM_024978253.1	XP_024834021.1	transcription intermediary factor 1-beta isoform X3
XM_024978251.1	XP_024834019.1	transcription intermediary factor 1-beta isoform X1
XM_024978254.1	XP_024834022.1	transcription intermediary factor 1-beta isoform X4
NM_001206809.1	NP_001193738.1	transcription intermediary factor 1-beta

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TRIM28	XP_533567.4
Xenopus tropicalis (tropical clawed frog)	LOC100489687	XP_004916264.1
	TRIM28	XP_001098702.2
Mus musculus (house mouse)	Trim28	NP_035718.2
Rattus norvegicus (Norway rat)	Trim28	NP_446368.1
Bos taurus (cattle)	TRIM28	NP_001193738.1
Homo sapiens (human)	TRIM28	NP_005753.1

Gene Ontology
Bibliography

Related articles in PubMed

In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures.
Rahmoun M, Lavery R, Laurent-Chaballier S, Bellora N, Philip GK, Rossitto M, Symon A, Pailhoux E, Cammas F, Chung J, Bagheri-Fam S, Murphy M, Bardwell V, Zarkower D, Boizet-Bonhoure B, Clair P, Harley VR, Poulat F
Nucleic acids research45(12)7191-7211(2017 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nuclear factors that bind to genomic regions with "Sertoli Cell Signature" could functionally interact with SOX9; TRIM28 is a new SOX9 partner in fetal testes.
Title: In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures.

The following TRIM28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIM28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa225014	XM_024978251.1 Latest version!	Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa225015	XM_024978252.1 Latest version!	Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa225016	XM_024978253.1 Latest version!	Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa225017	XM_024978254.1 Latest version!	Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa01875	NM_001206809.1 Latest version!	Bos taurus tripartite motif containing 28 (TRIM28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIM28 ( XM_024978251.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834019.1 Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa225014
Clone ID Related Accession (Same CDS sequence)	XM_024978251.1
Accession Version	XM_024978251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription intermediary factor 1-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTT CGGCTGCGGC GGCCTCGGCG GCGGCGGCGG CCGCCTCCGG CAGCCCCGGC 
CCGAGTGAGG GCTCGGCGGG CGCCGAGAAG CGCGCCGCCG CCTCCTCGGC CGCGGGCTCC 
GCATCGGGCT CGGCGGCGGC ATCCGCCTCG GCGTCGTCAC CCGCGGGGGG CGGCGGCGAG 
GCGCTGGAAC TGTTGGAGCA CTGCGGCGTG TGTCGGGAGC GCCTGCGGCC GGAGAGGGAG 
CCGCGCCTGC TACCTTGCCT GCACTCGGCC TGCAGCGCCT GCCTCGGGCC CCCGGCGCCT 
GCAGCCGCCA ACAGCTCGGG GGATGGAGGT GCAGCGGGCG ACGGTTCTGT GGTGGACTGT 
CCAGTATGTA AGCAGCAATG TTTCTCCAAA GATATCGTGG AGAATTATTT CATGCGAGAC 
TGTGGCGGTA AGGCCGCCAC GGATTCCCAG GATGCTAATC AGTGCTGCAC TAGCTGTGAG 
GATAATGCCC CAGCCACCAG TTATTGTGTG GAGTGCTCTG AGCCGCTCTG TGAGACGTGT 
GTGGAGGCGC ACCAGCGGGT GAAGTACACC AAGGACCACA CCGTGCGCTC CACTGAGAAG 
AGAGAAGAGG TCTCCAGGCG TGGCTCAGGA GGAAAAGGGC CGGCCAAGTC GAGGGACGGT 
GAGCGCACTG TGTATTGCAA TGTGCACAAG CACGAGCCCC TTGTGCTGTT CTGCGAGAGC 
TGCGACACCC TCACCTGCCG GGATTGCCAG CTCAACGCCC ACAAAGACCA CCAGTACCAG 
TTCCTGGAGG ATGCAGTGAG AAACCAGCGC AAGCTCCTGG CCTCATTGGT GAAGCGCCTC 
GGGGACAAGC ATGCAACACT GCAGAAGAAC ACCAAGGAGG TTCGCAGCTC GATCCGCCAG 
GTGTCCGACG TACAGAAGCG CGTGCAAGTG GATGTTAAGA TGGCCATCCT GCAGATAATG 
AAGGAACTGA ACAAGCGGGG CCGTGTGCTG GTCAACGACG CCCAGAAGGT AACAGAGGGG 
CAGCAGGAGC GCCTGGAACG GCAGCACTGG ACCATGACCA AGATCCAGAA GCACCAGGAA 
CACATCTTAC GCTTTGCCTC TTGGGCTCTG GAGAGTGATA ACAACACGGC TCTGCTGCTC 
TCCAAGAAGC TGATCTACTT CCAGCTGCAC CGGGCCCTTA AGATGATCGT GGACCCCGTG 
GAGCCCCACG GCGAGATGAA ATTTCAGTGG GACCTCAACG CCTGGACCAA GAGTGCAGAG 
GCCTTTGGCA AGATTGTTGC AGAGCGTCCT GGAACCAACT CCACGGGCCC AGCACCAATG 
GCCCCTCCTC GGGCTCCGGG GCCTTTGGGC AAGCAGGGCT CTGGCAGCAG CCAGCCTATG 
GAGGTGCAGG AAGGCTACGG CTTCGGATCA GATGACCCTT ACTCGAGTGC TGAGCCCCAT 
GTGTCAGGGG TGAAGCGACC CCGCTCAGGT GACGGCGAGG TGAGTGGCCT CATGCGCAAG 
GTGCCTCGGG TGAGCCTCGA ACGCCTGGAC TTGGATCTCA CGGCTGACAG CCAGCCACCA 
GTCTTTAAGG TCTTCCCAGG CAACACCACT GAGGATTACA ACCTCATCGT CATCGAGCGA 
GGTGCTGCCG CCGCTGCTGC TGGCCAGCCT GGGACTGCGC CCTCGGGGGT CCCTGGGGCC 
CCTCCCCTGC CTGGAATGGC CATTGTCAAG GAGGAAGAAA CAGAGGCTGC CATTGGAGCT 
CCACCAGCTG CCACCGAGGG CCCGGAGACC AAACCTGTGT TGATGGCCCT GGGGGAGGGC 
CCTGGTGCGG AGGGTCCCCG CCTGGCTTCA CCCAGTGGCA GCACCAGCTC AGGTCTGGAG 
GTGGTGGCTC CAGAGGGCAC CTCAGTCCCA GCTGGTGGCC CAGGTTCCCT GGATGACAGT 
GCCACCATCT GCCGTGTCTG CCAGAAGCCA GGTGACCTCG TCATGTGCAA CCAGTGCGAA 
TTCTGCTTCC ACCTGGACTG CCACCTGCCT GCCCTGCAGG ATGTGCCAGG GGAGGAGTGG 
AGCTGCTCTC TTTGCCACGT GCTGCCTGAC CTGAAGGAGG AGGATGGCAG CCTGAACCTG 
GATGGGGGGG ATAGTACCGG AGTGGTGGCC AAGCTCTCAC CAGCCAACCA GCAGAAGTGT 
GAGCGCGTCC TGCTGGCCCT CTTTTGCCAC GAGCCCTGCC GGCCTCTGCA CCAGCTGGCT 
ACTGACTCCA CCTTCTCCCC AGTGAGTTCT GGGGCTTGGG AGGGTGGGAG GTCCAGGGGC 
TCTTCGGCCT CACCTTCAGC CTGTGGCCTC TGCCTGCAGG ATCAGCCTGG TGACACCCTG 
GACTTGACCC TTATCCGAGC CCGCCTCCAG GAGAAGCTGT CACCCCCCTA CAGCTCCCCC 
CAGGAGTTTG CCCAGGATGT GGGCCGCATG TTCAAGCAGT TCAACAAGCT GACGGAGGAC 
AAGGCCGACG TGCAGTCCAT CATTGGCCTA CAGCGCTTCT TTGAGACCCG CATGAATGAG 
GCCTTCGGGG ACACCAAGTT CTCCGCTGTG CTGGTGGAGC CCCCGCCGCT GAGCCTGCCT 
GGTGCTGGCC TGAGTGCCCA GGACCTCTCT ACTGGCCCTG GTGAGGGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834019.1
CDS	346..2979
Translation	MAASAAAASAAAAAASGSPGPSEGSAGAEKRAAASSAAGSASGSAAASASASSPAGGGGEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPPAPAAANSSGDGGAAGDGSVVDCPVCKQQCFSKDIVENYFMRDCGGKAATDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTEKREEVSRRGSGGKGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLGKQGSGSSQPMEVQEGYGFGSDDPYSSAEPHVSGVKRPRSGDGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGNTTEDYNLIVIERGAAAAAAGQPGTAPSGVPGAPPLPGMAIVKEEETEAAIGAPPAATEGPETKPVLMALGEGPGAEGPRLASPSGSTSSGLEVVAPEGTSVPAGGPGSLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLNLDGGDSTGVVAKLSPANQQKCERVLLALFCHEPCRPLHQLATDSTFSPVSSGAWEGGRSRGSSASPSACGLCLQDQPGDTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPLSLPGAGLSAQDLSTGPGEGP

Target ORF information:

RefSeq Version	XM_024978251.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978251.1

ORF Insert Sequence:

ATGGCGGCTT CGGCTGCGGC GGCCTCGGCG GCGGCGGCGG CCGCCTCCGG CAGCCCCGGC 
CCGAGTGAGG GCTCGGCGGG CGCCGAGAAG CGCGCCGCCG CCTCCTCGGC CGCGGGCTCC 
GCATCGGGCT CGGCGGCGGC ATCCGCCTCG GCGTCGTCAC CCGCGGGGGG CGGCGGCGAG 
GCGCTGGAAC TGTTGGAGCA CTGCGGCGTG TGTCGGGAGC GCCTGCGGCC GGAGAGGGAG 
CCGCGCCTGC TACCTTGCCT GCACTCGGCC TGCAGCGCCT GCCTCGGGCC CCCGGCGCCT 
GCAGCCGCCA ACAGCTCGGG GGATGGAGGT GCAGCGGGCG ACGGTTCTGT GGTGGACTGT 
CCAGTATGTA AGCAGCAATG TTTCTCCAAA GATATCGTGG AGAATTATTT CATGCGAGAC 
TGTGGCGGTA AGGCCGCCAC GGATTCCCAG GATGCTAATC AGTGCTGCAC TAGCTGTGAG 
GATAATGCCC CAGCCACCAG TTATTGTGTG GAGTGCTCTG AGCCGCTCTG TGAGACGTGT 
GTGGAGGCGC ACCAGCGGGT GAAGTACACC AAGGACCACA CCGTGCGCTC CACTGAGAAG 
AGAGAAGAGG TCTCCAGGCG TGGCTCAGGA GGAAAAGGGC CGGCCAAGTC GAGGGACGGT 
GAGCGCACTG TGTATTGCAA TGTGCACAAG CACGAGCCCC TTGTGCTGTT CTGCGAGAGC 
TGCGACACCC TCACCTGCCG GGATTGCCAG CTCAACGCCC ACAAAGACCA CCAGTACCAG 
TTCCTGGAGG ATGCAGTGAG AAACCAGCGC AAGCTCCTGG CCTCATTGGT GAAGCGCCTC 
GGGGACAAGC ATGCAACACT GCAGAAGAAC ACCAAGGAGG TTCGCAGCTC GATCCGCCAG 
GTGTCCGACG TACAGAAGCG CGTGCAAGTG GATGTTAAGA TGGCCATCCT GCAGATAATG 
AAGGAACTGA ACAAGCGGGG CCGTGTGCTG GTCAACGACG CCCAGAAGGT AACAGAGGGG 
CAGCAGGAGC GCCTGGAACG GCAGCACTGG ACCATGACCA AGATCCAGAA GCACCAGGAA 
CACATCTTAC GCTTTGCCTC TTGGGCTCTG GAGAGTGATA ACAACACGGC TCTGCTGCTC 
TCCAAGAAGC TGATCTACTT CCAGCTGCAC CGGGCCCTTA AGATGATCGT GGACCCCGTG 
GAGCCCCACG GCGAGATGAA ATTTCAGTGG GACCTCAACG CCTGGACCAA GAGTGCAGAG 
GCCTTTGGCA AGATTGTTGC AGAGCGTCCT GGAACCAACT CCACGGGCCC AGCACCAATG 
GCCCCTCCTC GGGCTCCGGG GCCTTTGGGC AAGCAGGGCT CTGGCAGCAG CCAGCCTATG 
GAGGTGCAGG AAGGCTACGG CTTCGGATCA GATGACCCTT ACTCGAGTGC TGAGCCCCAT 
GTGTCAGGGG TGAAGCGACC CCGCTCAGGT GACGGCGAGG TGAGTGGCCT CATGCGCAAG 
GTGCCTCGGG TGAGCCTCGA ACGCCTGGAC TTGGATCTCA CGGCTGACAG CCAGCCACCA 
GTCTTTAAGG TCTTCCCAGG CAACACCACT GAGGATTACA ACCTCATCGT CATCGAGCGA 
GGTGCTGCCG CCGCTGCTGC TGGCCAGCCT GGGACTGCGC CCTCGGGGGT CCCTGGGGCC 
CCTCCCCTGC CTGGAATGGC CATTGTCAAG GAGGAAGAAA CAGAGGCTGC CATTGGAGCT 
CCACCAGCTG CCACCGAGGG CCCGGAGACC AAACCTGTGT TGATGGCCCT GGGGGAGGGC 
CCTGGTGCGG AGGGTCCCCG CCTGGCTTCA CCCAGTGGCA GCACCAGCTC AGGTCTGGAG 
GTGGTGGCTC CAGAGGGCAC CTCAGTCCCA GCTGGTGGCC CAGGTTCCCT GGATGACAGT 
GCCACCATCT GCCGTGTCTG CCAGAAGCCA GGTGACCTCG TCATGTGCAA CCAGTGCGAA 
TTCTGCTTCC ACCTGGACTG CCACCTGCCT GCCCTGCAGG ATGTGCCAGG GGAGGAGTGG 
AGCTGCTCTC TTTGCCACGT GCTGCCTGAC CTGAAGGAGG AGGATGGCAG CCTGAACCTG 
GATGGGGGGG ATAGTACCGG AGTGGTGGCC AAGCTCTCAC CAGCCAACCA GCAGAAGTGT 
GAGCGCGTCC TGCTGGCCCT CTTTTGCCAC GAGCCCTGCC GGCCTCTGCA CCAGCTGGCT 
ACTGACTCCA CCTTCTCCCC AGTGAGTTCT GGGGCTTGGG AGGGTGGGAG GTCCAGGGGC 
TCTTCGGCCT CACCTTCAGC CTGTGGCCTC TGCCTGCAGG ATCAGCCTGG TGACACCCTG 
GACTTGACCC TTATCCGAGC CCGCCTCCAG GAGAAGCTGT CACCCCCCTA CAGCTCCCCC 
CAGGAGTTTG CCCAGGATGT GGGCCGCATG TTCAAGCAGT TCAACAAGCT GACGGAGGAC 
AAGGCCGACG TGCAGTCCAT CATTGGCCTA CAGCGCTTCT TTGAGACCCG CATGAATGAG 
GCCTTCGGGG ACACCAAGTT CTCCGCTGTG CTGGTGGAGC CCCCGCCGCT GAGCCTGCCT 
GGTGCTGGCC TGAGTGCCCA GGACCTCTCT ACTGGCCCTG GTGAGGGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM28 ( XM_024978252.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834020.1 Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa225015
Clone ID Related Accession (Same CDS sequence)	XM_024978252.1
Accession Version	XM_024978252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription intermediary factor 1-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTT CGGCTGCGGC GGCCTCGGCG GCGGCGGCGG CCGCCTCCGG CAGCCCCGGC 
CCGAGTGAGG GCTCGGCGGG CGCCGAGAAG CGCGCCGCCG CCTCCTCGGC CGCGGGCTCC 
GCATCGGGCT CGGCGGCGGC ATCCGCCTCG GCGTCGTCAC CCGCGGGGGG CGGCGGCGAG 
GCGCTGGAAC TGTTGGAGCA CTGCGGCGTG TGTCGGGAGC GCCTGCGGCC GGAGAGGGAG 
CCGCGCCTGC TACCTTGCCT GCACTCGGCC TGCAGCGCCT GCCTCGGGCC CCCGGCGCCT 
GCAGCCGCCA ACAGCTCGGG GGATGGAGGT GCAGCGGGCG ACGGTTCTGT GGTGGACTGT 
CCAGTATGTA AGCAGCAATG TTTCTCCAAA GATATCGTGG AGAATTATTT CATGCGAGAC 
TGTGGCGGTA AGGCCGCCAC GGATTCCCAG GATGCTAATC AGTGCTGCAC TAGCTGTGAG 
GATAATGCCC CAGCCACCAG TTATTGTGTG GAGTGCTCTG AGCCGCTCTG TGAGACGTGT 
GTGGAGGCGC ACCAGCGGGT GAAGTACACC AAGGACCACA CCGTGCGCTC CACTGGGCCG 
GCCAAGTCGA GGGACGGTGA GCGCACTGTG TATTGCAATG TGCACAAGCA CGAGCCCCTT 
GTGCTGTTCT GCGAGAGCTG CGACACCCTC ACCTGCCGGG ATTGCCAGCT CAACGCCCAC 
AAAGACCACC AGTACCAGTT CCTGGAGGAT GCAGTGAGAA ACCAGCGCAA GCTCCTGGCC 
TCATTGGTGA AGCGCCTCGG GGACAAGCAT GCAACACTGC AGAAGAACAC CAAGGAGGTT 
CGCAGCTCGA TCCGCCAGGT GTCCGACGTA CAGAAGCGCG TGCAAGTGGA TGTTAAGATG 
GCCATCCTGC AGATAATGAA GGAACTGAAC AAGCGGGGCC GTGTGCTGGT CAACGACGCC 
CAGAAGGTAA CAGAGGGGCA GCAGGAGCGC CTGGAACGGC AGCACTGGAC CATGACCAAG 
ATCCAGAAGC ACCAGGAACA CATCTTACGC TTTGCCTCTT GGGCTCTGGA GAGTGATAAC 
AACACGGCTC TGCTGCTCTC CAAGAAGCTG ATCTACTTCC AGCTGCACCG GGCCCTTAAG 
ATGATCGTGG ACCCCGTGGA GCCCCACGGC GAGATGAAAT TTCAGTGGGA CCTCAACGCC 
TGGACCAAGA GTGCAGAGGC CTTTGGCAAG ATTGTTGCAG AGCGTCCTGG AACCAACTCC 
ACGGGCCCAG CACCAATGGC CCCTCCTCGG GCTCCGGGGC CTTTGGGCAA GCAGGGCTCT 
GGCAGCAGCC AGCCTATGGA GGTGCAGGAA GGCTACGGCT TCGGATCAGA TGACCCTTAC 
TCGAGTGCTG AGCCCCATGT GTCAGGGGTG AAGCGACCCC GCTCAGGTGA CGGCGAGGTG 
AGTGGCCTCA TGCGCAAGGT GCCTCGGGTG AGCCTCGAAC GCCTGGACTT GGATCTCACG 
GCTGACAGCC AGCCACCAGT CTTTAAGGTC TTCCCAGGCA ACACCACTGA GGATTACAAC 
CTCATCGTCA TCGAGCGAGG TGCTGCCGCC GCTGCTGCTG GCCAGCCTGG GACTGCGCCC 
TCGGGGGTCC CTGGGGCCCC TCCCCTGCCT GGAATGGCCA TTGTCAAGGA GGAAGAAACA 
GAGGCTGCCA TTGGAGCTCC ACCAGCTGCC ACCGAGGGCC CGGAGACCAA ACCTGTGTTG 
ATGGCCCTGG GGGAGGGCCC TGGTGCGGAG GGTCCCCGCC TGGCTTCACC CAGTGGCAGC 
ACCAGCTCAG GTCTGGAGGT GGTGGCTCCA GAGGGCACCT CAGTCCCAGC TGGTGGCCCA 
GGTTCCCTGG ATGACAGTGC CACCATCTGC CGTGTCTGCC AGAAGCCAGG TGACCTCGTC 
ATGTGCAACC AGTGCGAATT CTGCTTCCAC CTGGACTGCC ACCTGCCTGC CCTGCAGGAT 
GTGCCAGGGG AGGAGTGGAG CTGCTCTCTT TGCCACGTGC TGCCTGACCT GAAGGAGGAG 
GATGGCAGCC TGAACCTGGA TGGGGGGGAT AGTACCGGAG TGGTGGCCAA GCTCTCACCA 
GCCAACCAGC AGAAGTGTGA GCGCGTCCTG CTGGCCCTCT TTTGCCACGA GCCCTGCCGG 
CCTCTGCACC AGCTGGCTAC TGACTCCACC TTCTCCCCAG TGAGTTCTGG GGCTTGGGAG 
GGTGGGAGGT CCAGGGGCTC TTCGGCCTCA CCTTCAGCCT GTGGCCTCTG CCTGCAGGAT 
CAGCCTGGTG ACACCCTGGA CTTGACCCTT ATCCGAGCCC GCCTCCAGGA GAAGCTGTCA 
CCCCCCTACA GCTCCCCCCA GGAGTTTGCC CAGGATGTGG GCCGCATGTT CAAGCAGTTC 
AACAAGCTGA CGGAGGACAA GGCCGACGTG CAGTCCATCA TTGGCCTACA GCGCTTCTTT 
GAGACCCGCA TGAATGAGGC CTTCGGGGAC ACCAAGTTCT CCGCTGTGCT GGTGGAGCCC 
CCGCCGCTGA GCCTGCCTGG TGCTGGCCTG AGTGCCCAGG ACCTCTCTAC TGGCCCTGGT 
GAGGGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834020.1
CDS	346..2937
Translation	MAASAAAASAAAAAASGSPGPSEGSAGAEKRAAASSAAGSASGSAAASASASSPAGGGGEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPPAPAAANSSGDGGAAGDGSVVDCPVCKQQCFSKDIVENYFMRDCGGKAATDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLGKQGSGSSQPMEVQEGYGFGSDDPYSSAEPHVSGVKRPRSGDGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGNTTEDYNLIVIERGAAAAAAGQPGTAPSGVPGAPPLPGMAIVKEEETEAAIGAPPAATEGPETKPVLMALGEGPGAEGPRLASPSGSTSSGLEVVAPEGTSVPAGGPGSLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLNLDGGDSTGVVAKLSPANQQKCERVLLALFCHEPCRPLHQLATDSTFSPVSSGAWEGGRSRGSSASPSACGLCLQDQPGDTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPLSLPGAGLSAQDLSTGPGEGP

Target ORF information:

RefSeq Version	XM_024978252.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978252.1

ORF Insert Sequence:

ATGGCGGCTT CGGCTGCGGC GGCCTCGGCG GCGGCGGCGG CCGCCTCCGG CAGCCCCGGC 
CCGAGTGAGG GCTCGGCGGG CGCCGAGAAG CGCGCCGCCG CCTCCTCGGC CGCGGGCTCC 
GCATCGGGCT CGGCGGCGGC ATCCGCCTCG GCGTCGTCAC CCGCGGGGGG CGGCGGCGAG 
GCGCTGGAAC TGTTGGAGCA CTGCGGCGTG TGTCGGGAGC GCCTGCGGCC GGAGAGGGAG 
CCGCGCCTGC TACCTTGCCT GCACTCGGCC TGCAGCGCCT GCCTCGGGCC CCCGGCGCCT 
GCAGCCGCCA ACAGCTCGGG GGATGGAGGT GCAGCGGGCG ACGGTTCTGT GGTGGACTGT 
CCAGTATGTA AGCAGCAATG TTTCTCCAAA GATATCGTGG AGAATTATTT CATGCGAGAC 
TGTGGCGGTA AGGCCGCCAC GGATTCCCAG GATGCTAATC AGTGCTGCAC TAGCTGTGAG 
GATAATGCCC CAGCCACCAG TTATTGTGTG GAGTGCTCTG AGCCGCTCTG TGAGACGTGT 
GTGGAGGCGC ACCAGCGGGT GAAGTACACC AAGGACCACA CCGTGCGCTC CACTGGGCCG 
GCCAAGTCGA GGGACGGTGA GCGCACTGTG TATTGCAATG TGCACAAGCA CGAGCCCCTT 
GTGCTGTTCT GCGAGAGCTG CGACACCCTC ACCTGCCGGG ATTGCCAGCT CAACGCCCAC 
AAAGACCACC AGTACCAGTT CCTGGAGGAT GCAGTGAGAA ACCAGCGCAA GCTCCTGGCC 
TCATTGGTGA AGCGCCTCGG GGACAAGCAT GCAACACTGC AGAAGAACAC CAAGGAGGTT 
CGCAGCTCGA TCCGCCAGGT GTCCGACGTA CAGAAGCGCG TGCAAGTGGA TGTTAAGATG 
GCCATCCTGC AGATAATGAA GGAACTGAAC AAGCGGGGCC GTGTGCTGGT CAACGACGCC 
CAGAAGGTAA CAGAGGGGCA GCAGGAGCGC CTGGAACGGC AGCACTGGAC CATGACCAAG 
ATCCAGAAGC ACCAGGAACA CATCTTACGC TTTGCCTCTT GGGCTCTGGA GAGTGATAAC 
AACACGGCTC TGCTGCTCTC CAAGAAGCTG ATCTACTTCC AGCTGCACCG GGCCCTTAAG 
ATGATCGTGG ACCCCGTGGA GCCCCACGGC GAGATGAAAT TTCAGTGGGA CCTCAACGCC 
TGGACCAAGA GTGCAGAGGC CTTTGGCAAG ATTGTTGCAG AGCGTCCTGG AACCAACTCC 
ACGGGCCCAG CACCAATGGC CCCTCCTCGG GCTCCGGGGC CTTTGGGCAA GCAGGGCTCT 
GGCAGCAGCC AGCCTATGGA GGTGCAGGAA GGCTACGGCT TCGGATCAGA TGACCCTTAC 
TCGAGTGCTG AGCCCCATGT GTCAGGGGTG AAGCGACCCC GCTCAGGTGA CGGCGAGGTG 
AGTGGCCTCA TGCGCAAGGT GCCTCGGGTG AGCCTCGAAC GCCTGGACTT GGATCTCACG 
GCTGACAGCC AGCCACCAGT CTTTAAGGTC TTCCCAGGCA ACACCACTGA GGATTACAAC 
CTCATCGTCA TCGAGCGAGG TGCTGCCGCC GCTGCTGCTG GCCAGCCTGG GACTGCGCCC 
TCGGGGGTCC CTGGGGCCCC TCCCCTGCCT GGAATGGCCA TTGTCAAGGA GGAAGAAACA 
GAGGCTGCCA TTGGAGCTCC ACCAGCTGCC ACCGAGGGCC CGGAGACCAA ACCTGTGTTG 
ATGGCCCTGG GGGAGGGCCC TGGTGCGGAG GGTCCCCGCC TGGCTTCACC CAGTGGCAGC 
ACCAGCTCAG GTCTGGAGGT GGTGGCTCCA GAGGGCACCT CAGTCCCAGC TGGTGGCCCA 
GGTTCCCTGG ATGACAGTGC CACCATCTGC CGTGTCTGCC AGAAGCCAGG TGACCTCGTC 
ATGTGCAACC AGTGCGAATT CTGCTTCCAC CTGGACTGCC ACCTGCCTGC CCTGCAGGAT 
GTGCCAGGGG AGGAGTGGAG CTGCTCTCTT TGCCACGTGC TGCCTGACCT GAAGGAGGAG 
GATGGCAGCC TGAACCTGGA TGGGGGGGAT AGTACCGGAG TGGTGGCCAA GCTCTCACCA 
GCCAACCAGC AGAAGTGTGA GCGCGTCCTG CTGGCCCTCT TTTGCCACGA GCCCTGCCGG 
CCTCTGCACC AGCTGGCTAC TGACTCCACC TTCTCCCCAG TGAGTTCTGG GGCTTGGGAG 
GGTGGGAGGT CCAGGGGCTC TTCGGCCTCA CCTTCAGCCT GTGGCCTCTG CCTGCAGGAT 
CAGCCTGGTG ACACCCTGGA CTTGACCCTT ATCCGAGCCC GCCTCCAGGA GAAGCTGTCA 
CCCCCCTACA GCTCCCCCCA GGAGTTTGCC CAGGATGTGG GCCGCATGTT CAAGCAGTTC 
AACAAGCTGA CGGAGGACAA GGCCGACGTG CAGTCCATCA TTGGCCTACA GCGCTTCTTT 
GAGACCCGCA TGAATGAGGC CTTCGGGGAC ACCAAGTTCT CCGCTGTGCT GGTGGAGCCC 
CCGCCGCTGA GCCTGCCTGG TGCTGGCCTG AGTGCCCAGG ACCTCTCTAC TGGCCCTGGT 
GAGGGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM28 ( XM_024978253.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834021.1 Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa225016
Clone ID Related Accession (Same CDS sequence)	XM_024978253.1
Accession Version	XM_024978253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription intermediary factor 1-beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTT CGGCTGCGGC GGCCTCGGCG GCGGCGGCGG CCGCCTCCGG CAGCCCCGGC 
CCGAGTGAGG GCTCGGCGGG CGCCGAGAAG CGCGCCGCCG CCTCCTCGGC CGCGGGCTCC 
GCATCGGGCT CGGCGGCGGC ATCCGCCTCG GCGTCGTCAC CCGCGGGGGG CGGCGGCGAG 
GCGCTGGAAC TGTTGGAGCA CTGCGGCGTG TGTCGGGAGC GCCTGCGGCC GGAGAGGGAG 
CCGCGCCTGC TACCTTGCCT GCACTCGGCC TGCAGCGCCT GCCTCGGGCC CCCGGCGCCT 
GCAGCCGCCA ACAGCTCGGG GGATGGAGGT GCAGCGGGCG ACGGTTCTGT GGTGGACTGT 
CCAGTATGTA AGCAGCAATG TTTCTCCAAA GATATCGTGG AGAATTATTT CATGCGAGAC 
TGTGGCGGTA AGGCCGCCAC GGATTCCCAG GATGCTAATC AGTGCTGCAC TAGCTGTGAG 
GATAATGCCC CAGCCACCAG TTATTGTGTG GAGTGCTCTG AGCCGCTCTG TGAGACGTGT 
GTGGAGGCGC ACCAGCGGGT GAAGTACACC AAGGACCACA CCGTGCGCTC CACTGAGAAG 
AGAGAAGAGG TCTCCAGGCG TGGCTCAGGA GGAAAAGGGC CGGCCAAGTC GAGGGACGGT 
GAGCGCACTG TGTATTGCAA TGTGCACAAG CACGAGCCCC TTGTGCTGTT CTGCGAGAGC 
TGCGACACCC TCACCTGCCG GGATTGCCAG CTCAACGCCC ACAAAGACCA CCAGTACCAG 
TTCCTGGAGG ATGCAGTGAG AAACCAGCGC AAGCTCCTGG CCTCATTGGT GAAGCGCCTC 
GGGGACAAGC ATGCAACACT GCAGAAGAAC ACCAAGGAGG TTCGCAGCTC GATCCGCCAG 
GTGTCCGACG TACAGAAGCG CGTGCAAGTG GATGTTAAGA TGGCCATCCT GCAGATAATG 
AAGGAACTGA ACAAGCGGGG CCGTGTGCTG GTCAACGACG CCCAGAAGGT AACAGAGGGG 
CAGCAGGAGC GCCTGGAACG GCAGCACTGG ACCATGACCA AGATCCAGAA GCACCAGGAA 
CACATCTTAC GCTTTGCCTC TTGGGCTCTG GAGAGTGATA ACAACACGGC TCTGCTGCTC 
TCCAAGAAGC TGATCTACTT CCAGCTGCAC CGGGCCCTTA AGATGATCGT GGACCCCGTG 
GAGCCCCACG GCGAGATGAA ATTTCAGTGG GACCTCAACG CCTGGACCAA GAGTGCAGAG 
GCCTTTGGCA AGATTGTTGC AGAGCGTCCT GGAACCAACT CCACGGGCCC AGCACCAATG 
GCCCCTCCTC GGGCTCCGGG GCCTTTGGGC AAGCAGGGCT CTGGCAGCAG CCAGCCTATG 
GAGGTGCAGG AAGGCTACGG CTTCGGATCA GATGACCCTT ACTCGAGTGC TGAGCCCCAT 
GTGTCAGGGG TGAAGCGACC CCGCTCAGGT GACGGCGAGG TGAGTGGCCT CATGCGCAAG 
GTGCCTCGGG TGAGCCTCGA ACGCCTGGAC TTGGATCTCA CGGCTGACAG CCAGCCACCA 
GTCTTTAAGG TCTTCCCAGG CAACACCACT GAGGATTACA ACCTCATCGT CATCGAGCGA 
GGTGCTGCCG CCGCTGCTGC TGGCCAGCCT GGGACTGCGC CCTCGGGGGT CCCTGGGGCC 
CCTCCCCTGC CTGGAATGGC CATTGTCAAG GAGGAAGAAA CAGAGGCTGC CATTGGAGCT 
CCACCAGCTG CCACCGAGGG CCCGGAGACC AAACCTGTGT TGATGGCCCT GGGGGAGGGC 
CCTGGTGCGG AGGGTCCCCG CCTGGCTTCA CCCAGTGGCA GCACCAGCTC AGGTCTGGAG 
GTGGTGGCTC CAGAGGGCAC CTCAGTCCCA GCTGGTGGCC CAGGTTCCCT GGATGACAGT 
GCCACCATCT GCCGTGTCTG CCAGAAGCCA GGTGACCTCG TCATGTGCAA CCAGTGCGAA 
TTCTGCTTCC ACCTGGACTG CCACCTGCCT GCCCTGCAGG ATGTGCCAGG GGAGGAGTGG 
AGCTGCTCTC TTTGCCACGT GCTGCCTGAC CTGAAGGAGG AGGATGGCAG CCTGAACCTG 
GATGGGGGGG ATAGTACCGG AGTGGTGGCC AAGCTCTCAC CAGCCAACCA GCAGAAGTGT 
GAGCGCGTCC TGCTGGCCCT CTTTTGCCAC GAGCCCTGCC GGCCTCTGCA CCAGCTGGCT 
ACTGACTCCA CCTTCTCCCC AGATCAGCCT GGTGACACCC TGGACTTGAC CCTTATCCGA 
GCCCGCCTCC AGGAGAAGCT GTCACCCCCC TACAGCTCCC CCCAGGAGTT TGCCCAGGAT 
GTGGGCCGCA TGTTCAAGCA GTTCAACAAG CTGACGGAGG ACAAGGCCGA CGTGCAGTCC 
ATCATTGGCC TACAGCGCTT CTTTGAGACC CGCATGAATG AGGCCTTCGG GGACACCAAG 
TTCTCCGCTG TGCTGGTGGA GCCCCCGCCG CTGAGCCTGC CTGGTGCTGG CCTGAGTGCC 
CAGGACCTCT CTACTGGCCC TGGTGAGGGC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834021.1
CDS	346..2901
Translation	MAASAAAASAAAAAASGSPGPSEGSAGAEKRAAASSAAGSASGSAAASASASSPAGGGGEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPPAPAAANSSGDGGAAGDGSVVDCPVCKQQCFSKDIVENYFMRDCGGKAATDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTEKREEVSRRGSGGKGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLGKQGSGSSQPMEVQEGYGFGSDDPYSSAEPHVSGVKRPRSGDGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGNTTEDYNLIVIERGAAAAAAGQPGTAPSGVPGAPPLPGMAIVKEEETEAAIGAPPAATEGPETKPVLMALGEGPGAEGPRLASPSGSTSSGLEVVAPEGTSVPAGGPGSLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLNLDGGDSTGVVAKLSPANQQKCERVLLALFCHEPCRPLHQLATDSTFSPDQPGDTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPLSLPGAGLSAQDLSTGPGEGP

Target ORF information:

RefSeq Version	XM_024978253.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978253.1

ORF Insert Sequence:

ATGGCGGCTT CGGCTGCGGC GGCCTCGGCG GCGGCGGCGG CCGCCTCCGG CAGCCCCGGC 
CCGAGTGAGG GCTCGGCGGG CGCCGAGAAG CGCGCCGCCG CCTCCTCGGC CGCGGGCTCC 
GCATCGGGCT CGGCGGCGGC ATCCGCCTCG GCGTCGTCAC CCGCGGGGGG CGGCGGCGAG 
GCGCTGGAAC TGTTGGAGCA CTGCGGCGTG TGTCGGGAGC GCCTGCGGCC GGAGAGGGAG 
CCGCGCCTGC TACCTTGCCT GCACTCGGCC TGCAGCGCCT GCCTCGGGCC CCCGGCGCCT 
GCAGCCGCCA ACAGCTCGGG GGATGGAGGT GCAGCGGGCG ACGGTTCTGT GGTGGACTGT 
CCAGTATGTA AGCAGCAATG TTTCTCCAAA GATATCGTGG AGAATTATTT CATGCGAGAC 
TGTGGCGGTA AGGCCGCCAC GGATTCCCAG GATGCTAATC AGTGCTGCAC TAGCTGTGAG 
GATAATGCCC CAGCCACCAG TTATTGTGTG GAGTGCTCTG AGCCGCTCTG TGAGACGTGT 
GTGGAGGCGC ACCAGCGGGT GAAGTACACC AAGGACCACA CCGTGCGCTC CACTGAGAAG 
AGAGAAGAGG TCTCCAGGCG TGGCTCAGGA GGAAAAGGGC CGGCCAAGTC GAGGGACGGT 
GAGCGCACTG TGTATTGCAA TGTGCACAAG CACGAGCCCC TTGTGCTGTT CTGCGAGAGC 
TGCGACACCC TCACCTGCCG GGATTGCCAG CTCAACGCCC ACAAAGACCA CCAGTACCAG 
TTCCTGGAGG ATGCAGTGAG AAACCAGCGC AAGCTCCTGG CCTCATTGGT GAAGCGCCTC 
GGGGACAAGC ATGCAACACT GCAGAAGAAC ACCAAGGAGG TTCGCAGCTC GATCCGCCAG 
GTGTCCGACG TACAGAAGCG CGTGCAAGTG GATGTTAAGA TGGCCATCCT GCAGATAATG 
AAGGAACTGA ACAAGCGGGG CCGTGTGCTG GTCAACGACG CCCAGAAGGT AACAGAGGGG 
CAGCAGGAGC GCCTGGAACG GCAGCACTGG ACCATGACCA AGATCCAGAA GCACCAGGAA 
CACATCTTAC GCTTTGCCTC TTGGGCTCTG GAGAGTGATA ACAACACGGC TCTGCTGCTC 
TCCAAGAAGC TGATCTACTT CCAGCTGCAC CGGGCCCTTA AGATGATCGT GGACCCCGTG 
GAGCCCCACG GCGAGATGAA ATTTCAGTGG GACCTCAACG CCTGGACCAA GAGTGCAGAG 
GCCTTTGGCA AGATTGTTGC AGAGCGTCCT GGAACCAACT CCACGGGCCC AGCACCAATG 
GCCCCTCCTC GGGCTCCGGG GCCTTTGGGC AAGCAGGGCT CTGGCAGCAG CCAGCCTATG 
GAGGTGCAGG AAGGCTACGG CTTCGGATCA GATGACCCTT ACTCGAGTGC TGAGCCCCAT 
GTGTCAGGGG TGAAGCGACC CCGCTCAGGT GACGGCGAGG TGAGTGGCCT CATGCGCAAG 
GTGCCTCGGG TGAGCCTCGA ACGCCTGGAC TTGGATCTCA CGGCTGACAG CCAGCCACCA 
GTCTTTAAGG TCTTCCCAGG CAACACCACT GAGGATTACA ACCTCATCGT CATCGAGCGA 
GGTGCTGCCG CCGCTGCTGC TGGCCAGCCT GGGACTGCGC CCTCGGGGGT CCCTGGGGCC 
CCTCCCCTGC CTGGAATGGC CATTGTCAAG GAGGAAGAAA CAGAGGCTGC CATTGGAGCT 
CCACCAGCTG CCACCGAGGG CCCGGAGACC AAACCTGTGT TGATGGCCCT GGGGGAGGGC 
CCTGGTGCGG AGGGTCCCCG CCTGGCTTCA CCCAGTGGCA GCACCAGCTC AGGTCTGGAG 
GTGGTGGCTC CAGAGGGCAC CTCAGTCCCA GCTGGTGGCC CAGGTTCCCT GGATGACAGT 
GCCACCATCT GCCGTGTCTG CCAGAAGCCA GGTGACCTCG TCATGTGCAA CCAGTGCGAA 
TTCTGCTTCC ACCTGGACTG CCACCTGCCT GCCCTGCAGG ATGTGCCAGG GGAGGAGTGG 
AGCTGCTCTC TTTGCCACGT GCTGCCTGAC CTGAAGGAGG AGGATGGCAG CCTGAACCTG 
GATGGGGGGG ATAGTACCGG AGTGGTGGCC AAGCTCTCAC CAGCCAACCA GCAGAAGTGT 
GAGCGCGTCC TGCTGGCCCT CTTTTGCCAC GAGCCCTGCC GGCCTCTGCA CCAGCTGGCT 
ACTGACTCCA CCTTCTCCCC AGATCAGCCT GGTGACACCC TGGACTTGAC CCTTATCCGA 
GCCCGCCTCC AGGAGAAGCT GTCACCCCCC TACAGCTCCC CCCAGGAGTT TGCCCAGGAT 
GTGGGCCGCA TGTTCAAGCA GTTCAACAAG CTGACGGAGG ACAAGGCCGA CGTGCAGTCC 
ATCATTGGCC TACAGCGCTT CTTTGAGACC CGCATGAATG AGGCCTTCGG GGACACCAAG 
TTCTCCGCTG TGCTGGTGGA GCCCCCGCCG CTGAGCCTGC CTGGTGCTGG CCTGAGTGCC 
CAGGACCTCT CTACTGGCCC TGGTGAGGGC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM28 ( XM_024978254.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834022.1 Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa225017
Clone ID Related Accession (Same CDS sequence)	XM_024978254.1
Accession Version	XM_024978254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcription intermediary factor 1-beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTT CGGCTGCGGC GGCCTCGGCG GCGGCGGCGG CCGCCTCCGG CAGCCCCGGC 
CCGAGTGAGG GCTCGGCGGG CGCCGAGAAG CGCGCCGCCG CCTCCTCGGC CGCGGGCTCC 
GCATCGGGCT CGGCGGCGGC ATCCGCCTCG GCGTCGTCAC CCGCGGGGGG CGGCGGCGAG 
GCGCTGGAAC TGTTGGAGCA CTGCGGCGTG TGTCGGGAGC GCCTGCGGCC GGAGAGGGAG 
CCGCGCCTGC TACCTTGCCT GCACTCGGCC TGCAGCGCCT GCCTCGGGCC CCCGGCGCCT 
GCAGCCGCCA ACAGCTCGGG GGATGGAGGT GCAGCGGGCG ACGGTTCTGT GGTGGACTGT 
CCAGTATGTA AGCAGCAATG TTTCTCCAAA GATATCGTGG AGAATTATTT CATGCGAGAC 
TGTGGCGGTA AGGCCGCCAC GGATTCCCAG GATGCTAATC AGTGCTGCAC TAGCTGTGAG 
GATAATGCCC CAGCCACCAG TTATTGTGTG GAGTGCTCTG AGCCGCTCTG TGAGACGTGT 
GTGGAGGCGC ACCAGCGGGT GAAGTACACC AAGGACCACA CCGTGCGCTC CACTGGGCCG 
GCCAAGTCGA GGGACGGTGA GCGCACTGTG TATTGCAATG TGCACAAGCA CGAGCCCCTT 
GTGCTGTTCT GCGAGAGCTG CGACACCCTC ACCTGCCGGG ATTGCCAGCT CAACGCCCAC 
AAAGACCACC AGTACCAGTT CCTGGAGGAT GCAGTGAGAA ACCAGCGCAA GCTCCTGGCC 
TCATTGGTGA AGCGCCTCGG GGACAAGCAT GCAACACTGC AGAAGAACAC CAAGGAGGTT 
CGCAGCTCGA TCCGCCAGGT GTCCGACGTA CAGAAGCGCG TGCAAGTGGA TGTTAAGATG 
GCCATCCTGC AGATAATGAA GGAACTGAAC AAGCGGGGCC GTGTGCTGGT CAACGACGCC 
CAGAAGGTAA CAGAGGGGCA GCAGGAGCGC CTGGAACGGC AGCACTGGAC CATGACCAAG 
ATCCAGAAGC ACCAGGAACA CATCTTACGC TTTGCCTCTT GGGCTCTGGA GAGTGATAAC 
AACACGGCTC TGCTGCTCTC CAAGAAGCTG ATCTACTTCC AGCTGCACCG GGCCCTTAAG 
ATGATCGTGG ACCCCGTGGA GCCCCACGGC GAGATGAAAT TTCAGTGGGA CCTCAACGCC 
TGGACCAAGA GTGCAGAGGC CTTTGGCAAG ATTGTTGCAG AGCGTCCTGG AACCAACTCC 
ACGGGCCCAG CACCAATGGC CCCTCCTCGG GCTCCGGGGC CTTTGGGCAA GCAGGGCTCT 
GGCAGCAGCC AGCCTATGGA GGTGCAGGAA GGCTACGGCT TCGGATCAGA TGACCCTTAC 
TCGAGTGCTG AGCCCCATGT GTCAGGGGTG AAGCGACCCC GCTCAGGTGA CGGCGAGGTG 
AGTGGCCTCA TGCGCAAGGT GCCTCGGGTG AGCCTCGAAC GCCTGGACTT GGATCTCACG 
GCTGACAGCC AGCCACCAGT CTTTAAGGTC TTCCCAGGCA ACACCACTGA GGATTACAAC 
CTCATCGTCA TCGAGCGAGG TGCTGCCGCC GCTGCTGCTG GCCAGCCTGG GACTGCGCCC 
TCGGGGGTCC CTGGGGCCCC TCCCCTGCCT GGAATGGCCA TTGTCAAGGA GGAAGAAACA 
GAGGCTGCCA TTGGAGCTCC ACCAGCTGCC ACCGAGGGCC CGGAGACCAA ACCTGTGTTG 
ATGGCCCTGG GGGAGGGCCC TGGTGCGGAG GGTCCCCGCC TGGCTTCACC CAGTGGCAGC 
ACCAGCTCAG GTCTGGAGGT GGTGGCTCCA GAGGGCACCT CAGTCCCAGC TGGTGGCCCA 
GGTTCCCTGG ATGACAGTGC CACCATCTGC CGTGTCTGCC AGAAGCCAGG TGACCTCGTC 
ATGTGCAACC AGTGCGAATT CTGCTTCCAC CTGGACTGCC ACCTGCCTGC CCTGCAGGAT 
GTGCCAGGGG AGGAGTGGAG CTGCTCTCTT TGCCACGTGC TGCCTGACCT GAAGGAGGAG 
GATGGCAGCC TGAACCTGGA TGGGGGGGAT AGTACCGGAG TGGTGGCCAA GCTCTCACCA 
GCCAACCAGC AGAAGTGTGA GCGCGTCCTG CTGGCCCTCT TTTGCCACGA GCCCTGCCGG 
CCTCTGCACC AGCTGGCTAC TGACTCCACC TTCTCCCCAG ATCAGCCTGG TGACACCCTG 
GACTTGACCC TTATCCGAGC CCGCCTCCAG GAGAAGCTGT CACCCCCCTA CAGCTCCCCC 
CAGGAGTTTG CCCAGGATGT GGGCCGCATG TTCAAGCAGT TCAACAAGCT GACGGAGGAC 
AAGGCCGACG TGCAGTCCAT CATTGGCCTA CAGCGCTTCT TTGAGACCCG CATGAATGAG 
GCCTTCGGGG ACACCAAGTT CTCCGCTGTG CTGGTGGAGC CCCCGCCGCT GAGCCTGCCT 
GGTGCTGGCC TGAGTGCCCA GGACCTCTCT ACTGGCCCTG GTGAGGGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834022.1
CDS	346..2859
Translation	MAASAAAASAAAAAASGSPGPSEGSAGAEKRAAASSAAGSASGSAAASASASSPAGGGGEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPPAPAAANSSGDGGAAGDGSVVDCPVCKQQCFSKDIVENYFMRDCGGKAATDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLGKQGSGSSQPMEVQEGYGFGSDDPYSSAEPHVSGVKRPRSGDGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGNTTEDYNLIVIERGAAAAAAGQPGTAPSGVPGAPPLPGMAIVKEEETEAAIGAPPAATEGPETKPVLMALGEGPGAEGPRLASPSGSTSSGLEVVAPEGTSVPAGGPGSLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLNLDGGDSTGVVAKLSPANQQKCERVLLALFCHEPCRPLHQLATDSTFSPDQPGDTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPLSLPGAGLSAQDLSTGPGEGP

Target ORF information:

RefSeq Version	XM_024978254.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tripartite motif containing 28 (TRIM28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978254.1

ORF Insert Sequence:

ATGGCGGCTT CGGCTGCGGC GGCCTCGGCG GCGGCGGCGG CCGCCTCCGG CAGCCCCGGC 
CCGAGTGAGG GCTCGGCGGG CGCCGAGAAG CGCGCCGCCG CCTCCTCGGC CGCGGGCTCC 
GCATCGGGCT CGGCGGCGGC ATCCGCCTCG GCGTCGTCAC CCGCGGGGGG CGGCGGCGAG 
GCGCTGGAAC TGTTGGAGCA CTGCGGCGTG TGTCGGGAGC GCCTGCGGCC GGAGAGGGAG 
CCGCGCCTGC TACCTTGCCT GCACTCGGCC TGCAGCGCCT GCCTCGGGCC CCCGGCGCCT 
GCAGCCGCCA ACAGCTCGGG GGATGGAGGT GCAGCGGGCG ACGGTTCTGT GGTGGACTGT 
CCAGTATGTA AGCAGCAATG TTTCTCCAAA GATATCGTGG AGAATTATTT CATGCGAGAC 
TGTGGCGGTA AGGCCGCCAC GGATTCCCAG GATGCTAATC AGTGCTGCAC TAGCTGTGAG 
GATAATGCCC CAGCCACCAG TTATTGTGTG GAGTGCTCTG AGCCGCTCTG TGAGACGTGT 
GTGGAGGCGC ACCAGCGGGT GAAGTACACC AAGGACCACA CCGTGCGCTC CACTGGGCCG 
GCCAAGTCGA GGGACGGTGA GCGCACTGTG TATTGCAATG TGCACAAGCA CGAGCCCCTT 
GTGCTGTTCT GCGAGAGCTG CGACACCCTC ACCTGCCGGG ATTGCCAGCT CAACGCCCAC 
AAAGACCACC AGTACCAGTT CCTGGAGGAT GCAGTGAGAA ACCAGCGCAA GCTCCTGGCC 
TCATTGGTGA AGCGCCTCGG GGACAAGCAT GCAACACTGC AGAAGAACAC CAAGGAGGTT 
CGCAGCTCGA TCCGCCAGGT GTCCGACGTA CAGAAGCGCG TGCAAGTGGA TGTTAAGATG 
GCCATCCTGC AGATAATGAA GGAACTGAAC AAGCGGGGCC GTGTGCTGGT CAACGACGCC 
CAGAAGGTAA CAGAGGGGCA GCAGGAGCGC CTGGAACGGC AGCACTGGAC CATGACCAAG 
ATCCAGAAGC ACCAGGAACA CATCTTACGC TTTGCCTCTT GGGCTCTGGA GAGTGATAAC 
AACACGGCTC TGCTGCTCTC CAAGAAGCTG ATCTACTTCC AGCTGCACCG GGCCCTTAAG 
ATGATCGTGG ACCCCGTGGA GCCCCACGGC GAGATGAAAT TTCAGTGGGA CCTCAACGCC 
TGGACCAAGA GTGCAGAGGC CTTTGGCAAG ATTGTTGCAG AGCGTCCTGG AACCAACTCC 
ACGGGCCCAG CACCAATGGC CCCTCCTCGG GCTCCGGGGC CTTTGGGCAA GCAGGGCTCT 
GGCAGCAGCC AGCCTATGGA GGTGCAGGAA GGCTACGGCT TCGGATCAGA TGACCCTTAC 
TCGAGTGCTG AGCCCCATGT GTCAGGGGTG AAGCGACCCC GCTCAGGTGA CGGCGAGGTG 
AGTGGCCTCA TGCGCAAGGT GCCTCGGGTG AGCCTCGAAC GCCTGGACTT GGATCTCACG 
GCTGACAGCC AGCCACCAGT CTTTAAGGTC TTCCCAGGCA ACACCACTGA GGATTACAAC 
CTCATCGTCA TCGAGCGAGG TGCTGCCGCC GCTGCTGCTG GCCAGCCTGG GACTGCGCCC 
TCGGGGGTCC CTGGGGCCCC TCCCCTGCCT GGAATGGCCA TTGTCAAGGA GGAAGAAACA 
GAGGCTGCCA TTGGAGCTCC ACCAGCTGCC ACCGAGGGCC CGGAGACCAA ACCTGTGTTG 
ATGGCCCTGG GGGAGGGCCC TGGTGCGGAG GGTCCCCGCC TGGCTTCACC CAGTGGCAGC 
ACCAGCTCAG GTCTGGAGGT GGTGGCTCCA GAGGGCACCT CAGTCCCAGC TGGTGGCCCA 
GGTTCCCTGG ATGACAGTGC CACCATCTGC CGTGTCTGCC AGAAGCCAGG TGACCTCGTC 
ATGTGCAACC AGTGCGAATT CTGCTTCCAC CTGGACTGCC ACCTGCCTGC CCTGCAGGAT 
GTGCCAGGGG AGGAGTGGAG CTGCTCTCTT TGCCACGTGC TGCCTGACCT GAAGGAGGAG 
GATGGCAGCC TGAACCTGGA TGGGGGGGAT AGTACCGGAG TGGTGGCCAA GCTCTCACCA 
GCCAACCAGC AGAAGTGTGA GCGCGTCCTG CTGGCCCTCT TTTGCCACGA GCCCTGCCGG 
CCTCTGCACC AGCTGGCTAC TGACTCCACC TTCTCCCCAG ATCAGCCTGG TGACACCCTG 
GACTTGACCC TTATCCGAGC CCGCCTCCAG GAGAAGCTGT CACCCCCCTA CAGCTCCCCC 
CAGGAGTTTG CCCAGGATGT GGGCCGCATG TTCAAGCAGT TCAACAAGCT GACGGAGGAC 
AAGGCCGACG TGCAGTCCAT CATTGGCCTA CAGCGCTTCT TTGAGACCCG CATGAATGAG 
GCCTTCGGGG ACACCAAGTT CTCCGCTGTG CTGGTGGAGC CCCCGCCGCT GAGCCTGCCT 
GGTGCTGGCC TGAGTGCCCA GGACCTCTCT ACTGGCCCTG GTGAGGGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM28 ( NM_001206809.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193738.1 Bos taurus tripartite motif containing 28 (TRIM28), mRNA.

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CloneID	OBa01875
Clone ID Related Accession (Same CDS sequence)	NM_001206809.1
Accession Version	NM_001206809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Bos taurus(cattle)
Product	transcription intermediary factor 1-beta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC148899.1 and DV818297.1. On or before May 11, 2011 this sequence version replaced XM_002702029.1, XM_002695515.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145491, SAMN03145494 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTT CGGCTGCGGC GGCCGCCTCC TCGGCCGCGG GCTCCGCATC GGGCTCGGCG 
GCGGCATCCG CCTCGGCGTC GTCACCCGCG GGGGGCGGCG GCGAGGCGCT GGAACTGTTG 
GAGCACTGCG GCGTGTGTCG GGAGCGCCTG CGGCCGGAGA GGGAGCCGCG CCTGCTACCT 
TGCCTGCACT CGGCCTGCAG CGCCTGCCTC GGGCCCCCGG CGCCTGCAGC CGCCAACAGC 
TCGGGGGATG GAGGTGCAGC GGGCGACGGT TCTGTGGTGG ACTGTCCAGT ATGTAAGCAG 
CAATGTTTCT CCAAAGATAT CGTGGAGAAT TATTTCAAGC GAGACTGTGG CGGTAAGGCC 
GCCACGGATT CCCAGGATGC TAATCAGTGC TGCACTAGCT GTGAGGATAA TGCCCCAGCC 
ACCAGTTATT GTGTGGAGTG CTCTGAGCCG CTCTGTGAGA CGTGTGTGGA GGCGCACCAG 
CGGGTGAAGT ACACCAAGGA CCACACCGTG CGCTCCACTG GGCCGGCCAA GTCGAGGGAC 
GGTGAGCGCA CTGTGTATTG CAATGTGCAC AAGCACGAGC CCCTTGTGCT GTTCTGCGAG 
AGCTGCGACA CCCTCACCTG CCGGGATTGC CAGCTCAACG CCCACAAAGA CCACCAGTAC 
CAGTTCCTGG AGGATGCAGT GAGAAACCAG CGCAAGCTCC TGGCCTCATT GGTGAAGCGC 
CTCGGGGACA AGCATGCAAC ACTGCAGAAG AACACCAAGG AGGTTCGCAG CTCGATCCGC 
CAGGTGTCCG ACGTACAGAA GCGCGTGCAA GTGGATGTTA AGATGGCCAT CCTGCAGATA 
ATGAAGGAAC TGAACAAGCG GGGCCGTGTG CTGGTCAACG ACGCCCAGAA GGTAACAGAG 
GGGCAGCAGG AGCGCCTGGA ACGGCAGCAC TGGACCATGA CCAAGATCCA GAAGCACCAG 
GAACACATCT TACGCTTTGC CTCTTGGGCT CTGGAGAGTG ATAACAACAC GGCTCTGCTG 
CTCTCCAAGA AGCTGATCTA CTTCCAGCTG CACCGGGCCC TTAAGATGAT CGTGGACCCC 
GTGGAGCCCC ACGGCGAGAT GAAATTTCAG TGGGACCTCA ACGCCTGGAC CAAGAGTGCA 
GAGGCCTTTG GCAAGATTGT TGCAGAGCGT CCTGGAACCA ACTCCACGGG CCCAGCACCA 
ATGGCCCCTC CTCGGGCTCC GGGGCCTTTG GGCAAGCAGG GCTCTGGCAG CAGCCAGCCT 
ATGGAGGTGC AGGAAGGCTA CGGCTTCGGA TCAGATGACC CTTACTCGAG TGCTGAGCCC 
CATGTGTCAG GGGTGAAGCG ACCCCGCTCA GGTGACGGCG AGGTGAGTGG CCTCATGCGC 
AAGGTGCCTC GGGTGAGCCT CGAACGCCTG GACTTGGATC TCACGGCTGA CAGCCAGCCA 
CCAGTCTTTA AGGTCTTCCC AGGCAACACC ACTGAGGATT ACAACCTCAT CGTCATCGAG 
CGAGGTGCTG CCGCCGCTGC TGCTGGCCAG CCTGGGACTG CGCCCTCGGG GGTCCCTGGG 
GCCCCTCCCC TGCCTGGAAT GGCCATTGTC AAGGAGGAAG AAACAGAGGC TGCCATTGGA 
GCTCCACCAG CTGCCACCGA GGGCCCGGAG ACCAAACCTG TGTTGATGGC CCTGGGGGAG 
GGCCCTGGTG CGGAGGGTCC CCGCCTGGCT TCACCCAGTG GCAGCACCAG CTCAGGTCTG 
GAGGTGGTGG CTCCAGAGGG CACCTCAGTC CCAGCTGGTG GCCCAGGTTC CCTGGATGAC 
AGTGCCACCA TCTGCCGTGT CTGCCAGAAG CCAGGTGACC TCGTCATGTG CAACCAGTGC 
GAATTCTGCT TCCACCTGGA CTGCCACCTG CCTGCCCTGC AGGATGTGCC AGGGGAGGAG 
TGGAGCTGCT CTCTTTGCCA CGTGCTGCCT GACCTGAAGG AGGAGGATGG CAGCCTGAAC 
CTGGATGGGG GGGATAGTAC CGGAGTGGTG GCCAAGCTCT CACCAGCCAA CCAGCAGAAG 
TGTGAGCGCG TCCTGCTGGC CCTCTTTTGC CACGAGCCCT GCCGGCCTCT GCACCAGCTG 
GCTACTGACT CCACCTTCTC CCCAGATCAG CCTGGTGACA CCCTGGACTT GACCCTTATC 
CGAGCCCGCC TCCAGGAGAA GCTGTCACCC CCCTACAGCT CCCCCCAGGA GTTTGCCCAG 
GATGTGGGCC GCATGTTCAA GCAGTTCAAC AAGCTGACGG AGGACAAGGC CGACGTGCAG 
TCCATCATTG GCCTACAGCG CTTCTTTGAG ACCCGCATGA ATGAGGCCTT CGGGGACACC 
AAGTTCTCCG CTGTGCTGGT GGAGCCCCCG CCGCTGAGCC TGCCTGGTGC TGGCCTGAGT 
GCCCAGGACC TCTCTACTGG CCCTGGTGAG GGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193738.1
CDS	351..2789
Translation	MAASAAAAASSAAGSASGSAAASASASSPAGGGGEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPPAPAAANSSGDGGAAGDGSVVDCPVCKQQCFSKDIVENYFKRDCGGKAATDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLGKQGSGSSQPMEVQEGYGFGSDDPYSSAEPHVSGVKRPRSGDGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGNTTEDYNLIVIERGAAAAAAGQPGTAPSGVPGAPPLPGMAIVKEEETEAAIGAPPAATEGPETKPVLMALGEGPGAEGPRLASPSGSTSSGLEVVAPEGTSVPAGGPGSLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLNLDGGDSTGVVAKLSPANQQKCERVLLALFCHEPCRPLHQLATDSTFSPDQPGDTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPLSLPGAGLSAQDLSTGPGEGP

Target ORF information:

RefSeq Version	NM_001206809.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tripartite motif containing 28 (TRIM28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206809.1

ORF Insert Sequence:

ATGGCGGCTT CGGCTGCGGC GGCCGCCTCC TCGGCCGCGG GCTCCGCATC GGGCTCGGCG 
GCGGCATCCG CCTCGGCGTC GTCACCCGCG GGGGGCGGCG GCGAGGCGCT GGAACTGTTG 
GAGCACTGCG GCGTGTGTCG GGAGCGCCTG CGGCCGGAGA GGGAGCCGCG CCTGCTACCT 
TGCCTGCACT CGGCCTGCAG CGCCTGCCTC GGGCCCCCGG CGCCTGCAGC CGCCAACAGC 
TCGGGGGATG GAGGTGCAGC GGGCGACGGT TCTGTGGTGG ACTGTCCAGT ATGTAAGCAG 
CAATGTTTCT CCAAAGATAT CGTGGAGAAT TATTTCAAGC GAGACTGTGG CGGTAAGGCC 
GCCACGGATT CCCAGGATGC TAATCAGTGC TGCACTAGCT GTGAGGATAA TGCCCCAGCC 
ACCAGTTATT GTGTGGAGTG CTCTGAGCCG CTCTGTGAGA CGTGTGTGGA GGCGCACCAG 
CGGGTGAAGT ACACCAAGGA CCACACCGTG CGCTCCACTG GGCCGGCCAA GTCGAGGGAC 
GGTGAGCGCA CTGTGTATTG CAATGTGCAC AAGCACGAGC CCCTTGTGCT GTTCTGCGAG 
AGCTGCGACA CCCTCACCTG CCGGGATTGC CAGCTCAACG CCCACAAAGA CCACCAGTAC 
CAGTTCCTGG AGGATGCAGT GAGAAACCAG CGCAAGCTCC TGGCCTCATT GGTGAAGCGC 
CTCGGGGACA AGCATGCAAC ACTGCAGAAG AACACCAAGG AGGTTCGCAG CTCGATCCGC 
CAGGTGTCCG ACGTACAGAA GCGCGTGCAA GTGGATGTTA AGATGGCCAT CCTGCAGATA 
ATGAAGGAAC TGAACAAGCG GGGCCGTGTG CTGGTCAACG ACGCCCAGAA GGTAACAGAG 
GGGCAGCAGG AGCGCCTGGA ACGGCAGCAC TGGACCATGA CCAAGATCCA GAAGCACCAG 
GAACACATCT TACGCTTTGC CTCTTGGGCT CTGGAGAGTG ATAACAACAC GGCTCTGCTG 
CTCTCCAAGA AGCTGATCTA CTTCCAGCTG CACCGGGCCC TTAAGATGAT CGTGGACCCC 
GTGGAGCCCC ACGGCGAGAT GAAATTTCAG TGGGACCTCA ACGCCTGGAC CAAGAGTGCA 
GAGGCCTTTG GCAAGATTGT TGCAGAGCGT CCTGGAACCA ACTCCACGGG CCCAGCACCA 
ATGGCCCCTC CTCGGGCTCC GGGGCCTTTG GGCAAGCAGG GCTCTGGCAG CAGCCAGCCT 
ATGGAGGTGC AGGAAGGCTA CGGCTTCGGA TCAGATGACC CTTACTCGAG TGCTGAGCCC 
CATGTGTCAG GGGTGAAGCG ACCCCGCTCA GGTGACGGCG AGGTGAGTGG CCTCATGCGC 
AAGGTGCCTC GGGTGAGCCT CGAACGCCTG GACTTGGATC TCACGGCTGA CAGCCAGCCA 
CCAGTCTTTA AGGTCTTCCC AGGCAACACC ACTGAGGATT ACAACCTCAT CGTCATCGAG 
CGAGGTGCTG CCGCCGCTGC TGCTGGCCAG CCTGGGACTG CGCCCTCGGG GGTCCCTGGG 
GCCCCTCCCC TGCCTGGAAT GGCCATTGTC AAGGAGGAAG AAACAGAGGC TGCCATTGGA 
GCTCCACCAG CTGCCACCGA GGGCCCGGAG ACCAAACCTG TGTTGATGGC CCTGGGGGAG 
GGCCCTGGTG CGGAGGGTCC CCGCCTGGCT TCACCCAGTG GCAGCACCAG CTCAGGTCTG 
GAGGTGGTGG CTCCAGAGGG CACCTCAGTC CCAGCTGGTG GCCCAGGTTC CCTGGATGAC 
AGTGCCACCA TCTGCCGTGT CTGCCAGAAG CCAGGTGACC TCGTCATGTG CAACCAGTGC 
GAATTCTGCT TCCACCTGGA CTGCCACCTG CCTGCCCTGC AGGATGTGCC AGGGGAGGAG 
TGGAGCTGCT CTCTTTGCCA CGTGCTGCCT GACCTGAAGG AGGAGGATGG CAGCCTGAAC 
CTGGATGGGG GGGATAGTAC CGGAGTGGTG GCCAAGCTCT CACCAGCCAA CCAGCAGAAG 
TGTGAGCGCG TCCTGCTGGC CCTCTTTTGC CACGAGCCCT GCCGGCCTCT GCACCAGCTG 
GCTACTGACT CCACCTTCTC CCCAGATCAG CCTGGTGACA CCCTGGACTT GACCCTTATC 
CGAGCCCGCC TCCAGGAGAA GCTGTCACCC CCCTACAGCT CCCCCCAGGA GTTTGCCCAG 
GATGTGGGCC GCATGTTCAA GCAGTTCAAC AAGCTGACGG AGGACAAGGC CGACGTGCAG 
TCCATCATTG GCCTACAGCG CTTCTTTGAG ACCCGCATGA ATGAGGCCTT CGGGGACACC 
AAGTTCTCCG CTGTGCTGGT GGAGCCCCCG CCGCTGAGCC TGCCTGGTGC TGGCCTGAGT 
GCCCAGGACC TCTCTACTGG CCCTGGTGAG GGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures.
Nucleic acids research45(12)7191-7211(2017 Jul)
Rahmoun M,Lavery R,Laurent-Chaballier S,Bellora N,Philip GK,Rossitto M,Symon A,Pailhoux E,Cammas F,Chung J,Bagheri-Fam S,Murphy M,Bardwell V,Zarkower D,Boizet-Bonhoure B,Clair P,Harley VR,Poulat F

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TRIM28 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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