Trim28 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim28
Entrez Gene ID	116698
Full Name	tripartite motif-containing 28
Synonyms	Kap1,Krip1,Tif1b
General protein information	Preferred Names tripartite motif-containing 28 Names transcription intermediary factor 1-beta E3 SUMO-protein ligase TRIM28 KRAB-associated protein 1 RING-type E3 ubiquitin transferase TIF1-beta TIF1-beta nuclear corepressor KAP-1 tripartite motif protein 28 tripartite motif-containing protein 28
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q12
Summary	mouse homolog is a transcriptional repressor; interacts with the Kruppel-associated box A (KRAB-A) domain of C2H2 zinc finger proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053916.1	NP_446368.1	transcription intermediary factor 1-beta
XM_006228108.3	XP_006228170.1	transcription intermediary factor 1-beta isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TRIM28	XP_533567.4
Xenopus tropicalis (tropical clawed frog)	LOC100489687	XP_004916264.1
	TRIM28	XP_001098702.2
Mus musculus (house mouse)	Trim28	NP_035718.2
Rattus norvegicus (Norway rat)	Trim28	NP_446368.1
Bos taurus (cattle)	TRIM28	NP_001193738.1
Homo sapiens (human)	TRIM28	NP_005753.1

Gene Ontology
Bibliography

Related articles in PubMed

ATRX binds to atypical chromatin domains at the 3' exons of zinc finger genes to preserve H3K9me3 enrichment.
Valle-Garc?a D, Qadeer ZA, McHugh DS, Ghiraldini FG, Chowdhury AH, Hasson D, Dyer MA, Recillas-Targa F, Bernstein E
Epigenetics11(6)398-414(2016 06)

A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype.
Serra RW, Fang M, Park SM, Hutchinson L, Green MR
eLife3e02313(2014 Mar)

Novel activity of KRAB domain that functions to reinforce nuclear localization of KRAB-containing zinc finger proteins by interacting with KAP1.
Wang W, Cai J, Wu Y, Hu L, Chen Z, Hu J, Chen Z, Li W, Guo M, Huang Z
Cellular and molecular life sciences : CMLS70(20)3947-58(2013 Oct)

The KRAB zinc finger protein RSL1 regulates sex- and tissue-specific promoter methylation and dynamic hormone-responsive chromatin configuration.
Krebs CJ, Schultz DC, Robins DM
Molecular and cellular biology32(18)3732-42(2012 Sep)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Trim28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11047	NM_053916.1 Latest version!	Rattus norvegicus tripartite motif-containing 28 (Trim28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$489.30 $699.00
ORa22270	XM_006228108.3 Latest version!	Rattus norvegicus tripartite motif-containing 28 (Trim28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim28 ( NM_053916.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446368.1 Rattus norvegicus tripartite motif-containing 28 (Trim28), mRNA.

Price & Availability↑

CloneID	ORa11047
Clone ID Related Accession (Same CDS sequence)	NM_053916.1
Accession Version	NM_053916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	transcription intermediary factor 1-beta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC166843.1. On or before Oct 23, 2008 this sequence version replaced XM_344861.3, XM_001062234.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC166843.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCCT CGGCGGCGGC GGCGACTGCG GCGGCCTCGG CCGCGACGGC CGCGTCAGCG 
GCCTCTGGTA GCCCAGGGTC GGGCGAAGGC TCGGCGGGCG GTGAGAAGCG CCCCGCTGCC 
TCTTCAGCCG CGGCGGCCTC GGCCTCCGCG TCGTCCCCTG CGGGGGGCGG TGGCGAGGCG 
CAGGAGCTTT TGGAGCACTG CGGCGTGTGT CGCGAGCGCT TGCGGCCTGA GCGGGATCCT 
CGGCTGCTGC CCTGTCTGCA TTCGGCCTGT AGTGCCTGCC TTGGCCCCGC TACACCCGCC 
GCAGCTAATA ATTCGGGGGA TGGCGGCTCG GCGGGCGACG GCGCTATGGT GGATTGTCCA 
GTGTGCAAAC AACAGTGCTA CTCCAAAGAC ATCGTGGAGA ATTACTTTAT GCGTGATAGT 
GGCAGTAAGG CCTCTTCTGA TTCCCAGGAT GCTAACCAGT GCTGCACTAG CTGTGAAGAT 
AACGCCCCAG CCACTAGCTA TTGTGTGGAG TGCTCTGAAC CACTGTGTGA GACCTGTGTG 
GAGGCTCACC AGCGGGTGAA ATACACCAAG GATCACACTG TGCGCTCCAC GGGTCCTGCT 
AAGACTCGAG ATGGAGAACG AACAGTCTAT TGTAATGTGC ACAAGCATGA ACCCCTTGTG 
CTGTTTTGTG AGAGCTGTGA CACGCTCACT TGCCGGGACT GCCAACTCAA TGCTCACAAG 
GACCACCAGT ACCAATTCTT GGAAGATGCA GTAAGGAACC AACGTAAACT CTTGGCTTCA 
CTGGTGAAAC GTCTTGGGGA CAAACATGCC ACACTGCAGA AAAACACCAA GGAGGTTCGA 
AGCTCGATCC GCCAGGTGTC TGATGTGCAG AAGCGAGTGC AGGTTGATGT GAAGATGGCC 
ATTCTGCAGA TCATGAAGGA GCTGAATAAA CGGGGACGAG TTCTGGTCAA TGATGCCCAG 
AAGGTGACTG AGGGTCAGCA GGAACGTCTG GAGCGTCAGC ACTGGACCAT GACCAAAATC 
CAGAAGCACC AGGAACACAT TTTGCGCTTT GCCTCTTGGG CTTTGGAGAG TGATAACAAT 
ACAGCCCTCT TGCTCTCTAA GAAGCTGATT TATTTCCAGC TGCATCGGGC CCTCAAAATG 
ATTGTGGATC CTGTGGAGCC TCATGGTGAG ATGAAGTTTC AGTGGGATCT CAATGCCTGG 
ACCAAGAGTG CTGAAGCCTT TGGTAAGATT GTGGCTGAGC GTCCTGGTAC AAACTCCACG 
GGTCCTGGGC CCATGGCTCC TCCAAGAGCC CCAGGCCCCC TAAGCAAGCA AGGTTCTGGT 
AGTAGCCAGC CCATGGAAGT ACAAGAGGGC TATGGCTTTG GGACAGATGA TCCCTACTCA 
AGTGCAGAGC CACATGTATC AGGCATGAAG CGGTCCCGCT CTGGTGAGGG AGAAGTAAGT 
GGCCTCATGA GGAAGGTGCC ACGTGTGAGC CTTGAACGCC TGGATCTGGA TCTCACCTCT 
GATAGCCAGC CACCAGTCTT CAAGGTCTTT CCTGGAAGCA CCACTGAGGA CTACAATCTA 
ATTGTTATTG AGCGTGGTGC TGCTGCAGCA GCCGCTGGCC AGGCTGGGAC TGTGCCACCA 
GGAGCCCCTG GTGCCCCACC CCTGCCTGGC ATGGCCATTG TCAAGGAAGA AGAGACAGAA 
GCTGCTATTG GAGCCCCTCC TGCTGCCCCT GAGGGGCCTG AAACCAAGCC TGTGTTGATG 
GCTCTGACTG AAGGCCCTGG GGCTGAGGGA CCTCGTCTAG CTTCACCTAG TGGCAGTACC 
AGCTCAGGCT TGGAGGTGGT AGCTCCTGAG GTTACCTCAG CCCCAGTAAG TGGGCCAGGT 
ATCCTGGATG ACAGTGCCAC TATCTGCCGT GTATGCCAGA AGCCAGGTGA CCTGGTCATG 
TGCAACCAGT GCGAGTTTTG CTTCCACCTG GATTGTCACC TCCCTTCCCT GCAGGATGTT 
CCAGGGGAGG AATGGAGTTG CTCACTCTGC CACGTGCTCC CTGATCTAAA GGAGGAAGAT 
GGAAGCCTTA GCTTGGATGG AGCAGATAGC ACTGGTGTGG TAGCTAAACT CTCACCAGCC 
AACCAGCGGA AATGTGAGCG TGTTCTGCTG GCCCTTTTCT GCCATGAACC GTGCCGCCCC 
TTGCATCAGC TGGCTACCGA CTCTACATTC TCCATGGAGC AGCCTGGTGG TACCCTAGAC 
CTGACCTTGA TTCGTGCTCG CCTCCAAGAG AAGCTGTCAC CTCCTTATAG CTCCCCCCAG 
GAGTTTGCCC AGGATGTGGG CCGCATGTTC AAACAGTTCA ATAAGCTGAC TGAGGACAAG 
GCAGATGTTC AGTCCATCAT CGGCCTCCAG CGCTTCTTTG AGACACGAAT GAATGATGCC 
TTTGGTGACA CCAAGTTTTC TGCTGTGCTG GTAGAACCAC CACCACTGAA CCTTCCCAGT 
GCTGGCCTAA GTTCTCAGGA GCTGTCTGGT CCTGGTGATG GCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446368.1
CDS	284..2791
Translation	MAASAAAATAAASAATAASAASGSPGSGEGSAGGEKRPAASSAAAASASASSPAGGGGEAQELLEHCGVCRERLRPERDPRLLPCLHSACSACLGPATPAAANNSGDGGSAGDGAMVDCPVCKQQCYSKDIVENYFMRDSGSKASSDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKTRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPGPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGTDDPYSSAEPHVSGMKRSRSGEGEVSGLMRKVPRVSLERLDLDLTSDSQPPVFKVFPGSTTEDYNLIVIERGAAAAAAGQAGTVPPGAPGAPPLPGMAIVKEEETEAAIGAPPAAPEGPETKPVLMALTEGPGAEGPRLASPSGSTSSGLEVVAPEVTSAPVSGPGILDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPSLQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSMEQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNDAFGDTKFSAVLVEPPPLNLPSAGLSSQELSGPGDGP

Target ORF information:

RefSeq Version	NM_053916.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripartite motif-containing 28 (Trim28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053916.1

ORF Insert Sequence:

ATGGCGGCCT CGGCGGCGGC GGCGACTGCG GCGGCCTCGG CCGCGACGGC CGCGTCAGCG 
GCCTCTGGTA GCCCAGGGTC GGGCGAAGGC TCGGCGGGCG GTGAGAAGCG CCCCGCTGCC 
TCTTCAGCCG CGGCGGCCTC GGCCTCCGCG TCGTCCCCTG CGGGGGGCGG TGGCGAGGCG 
CAGGAGCTTT TGGAGCACTG CGGCGTGTGT CGCGAGCGCT TGCGGCCTGA GCGGGATCCT 
CGGCTGCTGC CCTGTCTGCA TTCGGCCTGT AGTGCCTGCC TTGGCCCCGC TACACCCGCC 
GCAGCTAATA ATTCGGGGGA TGGCGGCTCG GCGGGCGACG GCGCTATGGT GGATTGTCCA 
GTGTGCAAAC AACAGTGCTA CTCCAAAGAC ATCGTGGAGA ATTACTTTAT GCGTGATAGT 
GGCAGTAAGG CCTCTTCTGA TTCCCAGGAT GCTAACCAGT GCTGCACTAG CTGTGAAGAT 
AACGCCCCAG CCACTAGCTA TTGTGTGGAG TGCTCTGAAC CACTGTGTGA GACCTGTGTG 
GAGGCTCACC AGCGGGTGAA ATACACCAAG GATCACACTG TGCGCTCCAC GGGTCCTGCT 
AAGACTCGAG ATGGAGAACG AACAGTCTAT TGTAATGTGC ACAAGCATGA ACCCCTTGTG 
CTGTTTTGTG AGAGCTGTGA CACGCTCACT TGCCGGGACT GCCAACTCAA TGCTCACAAG 
GACCACCAGT ACCAATTCTT GGAAGATGCA GTAAGGAACC AACGTAAACT CTTGGCTTCA 
CTGGTGAAAC GTCTTGGGGA CAAACATGCC ACACTGCAGA AAAACACCAA GGAGGTTCGA 
AGCTCGATCC GCCAGGTGTC TGATGTGCAG AAGCGAGTGC AGGTTGATGT GAAGATGGCC 
ATTCTGCAGA TCATGAAGGA GCTGAATAAA CGGGGACGAG TTCTGGTCAA TGATGCCCAG 
AAGGTGACTG AGGGTCAGCA GGAACGTCTG GAGCGTCAGC ACTGGACCAT GACCAAAATC 
CAGAAGCACC AGGAACACAT TTTGCGCTTT GCCTCTTGGG CTTTGGAGAG TGATAACAAT 
ACAGCCCTCT TGCTCTCTAA GAAGCTGATT TATTTCCAGC TGCATCGGGC CCTCAAAATG 
ATTGTGGATC CTGTGGAGCC TCATGGTGAG ATGAAGTTTC AGTGGGATCT CAATGCCTGG 
ACCAAGAGTG CTGAAGCCTT TGGTAAGATT GTGGCTGAGC GTCCTGGTAC AAACTCCACG 
GGTCCTGGGC CCATGGCTCC TCCAAGAGCC CCAGGCCCCC TAAGCAAGCA AGGTTCTGGT 
AGTAGCCAGC CCATGGAAGT ACAAGAGGGC TATGGCTTTG GGACAGATGA TCCCTACTCA 
AGTGCAGAGC CACATGTATC AGGCATGAAG CGGTCCCGCT CTGGTGAGGG AGAAGTAAGT 
GGCCTCATGA GGAAGGTGCC ACGTGTGAGC CTTGAACGCC TGGATCTGGA TCTCACCTCT 
GATAGCCAGC CACCAGTCTT CAAGGTCTTT CCTGGAAGCA CCACTGAGGA CTACAATCTA 
ATTGTTATTG AGCGTGGTGC TGCTGCAGCA GCCGCTGGCC AGGCTGGGAC TGTGCCACCA 
GGAGCCCCTG GTGCCCCACC CCTGCCTGGC ATGGCCATTG TCAAGGAAGA AGAGACAGAA 
GCTGCTATTG GAGCCCCTCC TGCTGCCCCT GAGGGGCCTG AAACCAAGCC TGTGTTGATG 
GCTCTGACTG AAGGCCCTGG GGCTGAGGGA CCTCGTCTAG CTTCACCTAG TGGCAGTACC 
AGCTCAGGCT TGGAGGTGGT AGCTCCTGAG GTTACCTCAG CCCCAGTAAG TGGGCCAGGT 
ATCCTGGATG ACAGTGCCAC TATCTGCCGT GTATGCCAGA AGCCAGGTGA CCTGGTCATG 
TGCAACCAGT GCGAGTTTTG CTTCCACCTG GATTGTCACC TCCCTTCCCT GCAGGATGTT 
CCAGGGGAGG AATGGAGTTG CTCACTCTGC CACGTGCTCC CTGATCTAAA GGAGGAAGAT 
GGAAGCCTTA GCTTGGATGG AGCAGATAGC ACTGGTGTGG TAGCTAAACT CTCACCAGCC 
AACCAGCGGA AATGTGAGCG TGTTCTGCTG GCCCTTTTCT GCCATGAACC GTGCCGCCCC 
TTGCATCAGC TGGCTACCGA CTCTACATTC TCCATGGAGC AGCCTGGTGG TACCCTAGAC 
CTGACCTTGA TTCGTGCTCG CCTCCAAGAG AAGCTGTCAC CTCCTTATAG CTCCCCCCAG 
GAGTTTGCCC AGGATGTGGG CCGCATGTTC AAACAGTTCA ATAAGCTGAC TGAGGACAAG 
GCAGATGTTC AGTCCATCAT CGGCCTCCAG CGCTTCTTTG AGACACGAAT GAATGATGCC 
TTTGGTGACA CCAAGTTTTC TGCTGTGCTG GTAGAACCAC CACCACTGAA CCTTCCCAGT 
GCTGGCCTAA GTTCTCAGGA GCTGTCTGGT CCTGGTGATG GCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim28 ( XM_006228108.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228170.1 Rattus norvegicus tripartite motif-containing 28 (Trim28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22270
Clone ID Related Accession (Same CDS sequence)	XM_006228108.3
Accession Version	XM_006228108.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription intermediary factor 1-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228108.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CGGCGGCGGC GGCGACTGCG GCGGCCTCGG CCGCGACGGC CGCGTCAGCG 
GCCTCTGGTA GCCCAGGGTC GGGCGAAGGC TCGGCGGGCG GTGAGAAGCG CCCCGCTGCC 
TCTTCAGCCG CGGCGGCCTC GGCCTCCGCG TCGTCCCCTG CGGGGGGCGG TGGCGAGGCG 
CAGGAGCTTT TGGAGCACTG CGGCGTGTGT CGCGAGCGCT TGCGGCCTGA GCGGGATCCT 
CGGCTGCTGC CCTGTCTGCA TTCGGCCTGT AGTGCCTGCC TTGGCCCCGC TACACCCGCC 
GCAGCTAATA ATTCGGGGGA TGGCGGCTCG GCGGGCGACG GCGCTATGGT GGATTGTCCA 
GTGTGCAAAC AACAGTGCTA CTCCAAAGAC ATCGTGGAGA ATTACTTTAT GCGTGATAGT 
GGCAGTAAGG CCTCTTCTGA TTCCCAGGAT GCTAACCAGT GCTGCACTAG CTGTGAAGAT 
AACGCCCCAG CCACTAGCTA TTGTGTGGAG TGCTCTGAAC CACTGTGTGA GACCTGTGTG 
GAGGCTCACC AGCGGGTGAA ATACACCAAG GATCACACTG TGCGCTCCAC GGGTCCTGCT 
AAGACTCGAG ATGGAGAACG AACAGTCTAT TGTAATGTGC ACAAGCATGA ACCCCTTGTG 
CTGTTTTGTG AGAGCTGTGA CACGCTCACT TGCCGGGACT GCCAACTCAA TGCTCACAAG 
GACCACCAGT ACCAATTCTT GGAAGATGCA GTAAGGAACC AACGTAAACT CTTGGCTTCA 
CTGGTGAAAC GTCTTGGGGA CAAACATGCC ACACTGCAGA AAAACACCAA GGAGGTTCGA 
AGCTCGATCC GCCAGGTGTC TGATGTGCAG AAGCGAGTGC AGGTTGATGT GAAGATGGCC 
ATTCTGCAGA TCATGAAGGA GCTGAATAAA CGGGGACGAG TTCTGGTCAA TGATGCCCAG 
AAGGTGACTG AGGGTCAGCA GGAACGTCTG GAGCGTCAGC ACTGGACCAT GACCAAAATC 
CAGAAGCACC AGGAACACAT TTTGCGCTTT GCCTCTTGGG CTTTGGAGAG TGATAACAAT 
ACAGCCCTCT TGCTCTCTAA GAAGCTGATT TATTTCCAGC TGCATCGGGC CCTCAAAATG 
ATTGTGGATC CTGTGGAGCC TCATGGTGAG ATGAAGTTTC AGTGGGATCT CAATGCCTGG 
ACCAAGAGTG CTGAAGCCTT TGGTAAGATT GTGGCTGAGC GTCCTGGTAC AAACTCCACG 
GGTCCTGGGC CCATGGCTCC TCCAAGAGCC CCAGGCCCCC TAAGCAAGCA AGGTTCTGGT 
AGTAGCCAGC CCATGGAAGT ACAAGAGGGC TATGGCTTTG GGACAGATGA TCCCTACTCA 
AGTGCAGAGC CACATGTATC AGGCATGAAG CGGTCCCGCT CTGGTGAGGG AGAAGTAAGT 
GGCCTCATGA GGAAGGTGCC ACGTGTGAGC CTTGAACGCC TGGATCTGGA TCTCACCTCT 
GATAGCCAGC CACCAGTCTT CAAGGTCTTT CCTGGAAGCA CCACTGAGGA CTACAATCTA 
ATTGTTATTG AGCGTGGTGC TGCTGCAGCA GCCGCTGGCC AGGCTGGGAC TGTGCCACCA 
GGAGCCCCTG GTGCCCCACC CCTGCCTGGC ATGGCCATTG TCAAGGAAGA AGAGACAGAA 
GCTGCTATTG GAGCCCCTCC TGCTGCCCCT GAGGGGCCTG AAACCAAGCC TGTGTTGATG 
GCTCTGACTG AAGGCCCTGG GGCTGAGGGA CCTCGTCTAG CTTCACCTAG TGGCAGTACC 
AGCTCAGGCT TGGAGGTGGT AGCTCCTGAG GTTACCTCAG CCCCAGTAAG TGGGCCAGGT 
ATCCTGGATG ACAGTGCCAC TATCTGCCGT GTATGCCAGA AGCCAGGTGA CCTGGTCATG 
TGCAACCAGT GCGAGTTTTG CTTCCACCTG GATTGTCACC TCCCTTCCCT GCAGGATGTT 
CCAGGGGAGG AATGGAGTTG CTCACTCTGC CACGTGCTCC CTGATCTAAA GGAGGAAGAT 
GGAAGCCTTA GCTTGGATGG AGCAGATAGC ACTGGTGTGG TAGCTAAACT CTCACCAGCC 
AACCAGCGGA AATGTGAGCG TGTTCTGCTG GCCCTTTTCT GCCATGAACC GTGCCGCCCC 
TTGCATCAGC TGGCTACCGA CTCTACATTC TCCATGGTGA GTAGTCCCAG AATAGGAGAG 
GTTGCAGGTG TCAAGTTGGT AGCCCCACTC AATGTCTGTT GTCTCTCCCT GCAGGAGCAG 
CCTGGTGGTA CCCTAGACCT GACCTTGATT CGTGCTCGCC TCCAAGAGAA GCTGTCACCT 
CCTTATAGCT CCCCCCAGGA GTTTGCCCAG GATGTGGGCC GCATGTTCAA ACAGTTCAAT 
AAGCTGACTG AGGACAAGGC AGATGTTCAG TCCATCATCG GCCTCCAGCG CTTCTTTGAG 
ACACGAATGA ATGATGCCTT TGGTGACACC AAGTTTTCTG CTGTGCTGGT AGAACCACCA 
CCACTGAACC TTCCCAGTGC TGGCCTAAGT TCTCAGGAGC TGTCTGGTCC TGGTGATGGC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228170.1
CDS	280..2865
Translation	MAASAAAATAAASAATAASAASGSPGSGEGSAGGEKRPAASSAAAASASASSPAGGGGEAQELLEHCGVCRERLRPERDPRLLPCLHSACSACLGPATPAAANNSGDGGSAGDGAMVDCPVCKQQCYSKDIVENYFMRDSGSKASSDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKTRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPGPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGTDDPYSSAEPHVSGMKRSRSGEGEVSGLMRKVPRVSLERLDLDLTSDSQPPVFKVFPGSTTEDYNLIVIERGAAAAAAGQAGTVPPGAPGAPPLPGMAIVKEEETEAAIGAPPAAPEGPETKPVLMALTEGPGAEGPRLASPSGSTSSGLEVVAPEVTSAPVSGPGILDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPSLQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSMVSSPRIGEVAGVKLVAPLNVCCLSLQEQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNDAFGDTKFSAVLVEPPPLNLPSAGLSSQELSGPGDGP

Target ORF information:

RefSeq Version	XM_006228108.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripartite motif-containing 28 (Trim28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228108.3

ORF Insert Sequence:

ATGGCGGCCT CGGCGGCGGC GGCGACTGCG GCGGCCTCGG CCGCGACGGC CGCGTCAGCG 
GCCTCTGGTA GCCCAGGGTC GGGCGAAGGC TCGGCGGGCG GTGAGAAGCG CCCCGCTGCC 
TCTTCAGCCG CGGCGGCCTC GGCCTCCGCG TCGTCCCCTG CGGGGGGCGG TGGCGAGGCG 
CAGGAGCTTT TGGAGCACTG CGGCGTGTGT CGCGAGCGCT TGCGGCCTGA GCGGGATCCT 
CGGCTGCTGC CCTGTCTGCA TTCGGCCTGT AGTGCCTGCC TTGGCCCCGC TACACCCGCC 
GCAGCTAATA ATTCGGGGGA TGGCGGCTCG GCGGGCGACG GCGCTATGGT GGATTGTCCA 
GTGTGCAAAC AACAGTGCTA CTCCAAAGAC ATCGTGGAGA ATTACTTTAT GCGTGATAGT 
GGCAGTAAGG CCTCTTCTGA TTCCCAGGAT GCTAACCAGT GCTGCACTAG CTGTGAAGAT 
AACGCCCCAG CCACTAGCTA TTGTGTGGAG TGCTCTGAAC CACTGTGTGA GACCTGTGTG 
GAGGCTCACC AGCGGGTGAA ATACACCAAG GATCACACTG TGCGCTCCAC GGGTCCTGCT 
AAGACTCGAG ATGGAGAACG AACAGTCTAT TGTAATGTGC ACAAGCATGA ACCCCTTGTG 
CTGTTTTGTG AGAGCTGTGA CACGCTCACT TGCCGGGACT GCCAACTCAA TGCTCACAAG 
GACCACCAGT ACCAATTCTT GGAAGATGCA GTAAGGAACC AACGTAAACT CTTGGCTTCA 
CTGGTGAAAC GTCTTGGGGA CAAACATGCC ACACTGCAGA AAAACACCAA GGAGGTTCGA 
AGCTCGATCC GCCAGGTGTC TGATGTGCAG AAGCGAGTGC AGGTTGATGT GAAGATGGCC 
ATTCTGCAGA TCATGAAGGA GCTGAATAAA CGGGGACGAG TTCTGGTCAA TGATGCCCAG 
AAGGTGACTG AGGGTCAGCA GGAACGTCTG GAGCGTCAGC ACTGGACCAT GACCAAAATC 
CAGAAGCACC AGGAACACAT TTTGCGCTTT GCCTCTTGGG CTTTGGAGAG TGATAACAAT 
ACAGCCCTCT TGCTCTCTAA GAAGCTGATT TATTTCCAGC TGCATCGGGC CCTCAAAATG 
ATTGTGGATC CTGTGGAGCC TCATGGTGAG ATGAAGTTTC AGTGGGATCT CAATGCCTGG 
ACCAAGAGTG CTGAAGCCTT TGGTAAGATT GTGGCTGAGC GTCCTGGTAC AAACTCCACG 
GGTCCTGGGC CCATGGCTCC TCCAAGAGCC CCAGGCCCCC TAAGCAAGCA AGGTTCTGGT 
AGTAGCCAGC CCATGGAAGT ACAAGAGGGC TATGGCTTTG GGACAGATGA TCCCTACTCA 
AGTGCAGAGC CACATGTATC AGGCATGAAG CGGTCCCGCT CTGGTGAGGG AGAAGTAAGT 
GGCCTCATGA GGAAGGTGCC ACGTGTGAGC CTTGAACGCC TGGATCTGGA TCTCACCTCT 
GATAGCCAGC CACCAGTCTT CAAGGTCTTT CCTGGAAGCA CCACTGAGGA CTACAATCTA 
ATTGTTATTG AGCGTGGTGC TGCTGCAGCA GCCGCTGGCC AGGCTGGGAC TGTGCCACCA 
GGAGCCCCTG GTGCCCCACC CCTGCCTGGC ATGGCCATTG TCAAGGAAGA AGAGACAGAA 
GCTGCTATTG GAGCCCCTCC TGCTGCCCCT GAGGGGCCTG AAACCAAGCC TGTGTTGATG 
GCTCTGACTG AAGGCCCTGG GGCTGAGGGA CCTCGTCTAG CTTCACCTAG TGGCAGTACC 
AGCTCAGGCT TGGAGGTGGT AGCTCCTGAG GTTACCTCAG CCCCAGTAAG TGGGCCAGGT 
ATCCTGGATG ACAGTGCCAC TATCTGCCGT GTATGCCAGA AGCCAGGTGA CCTGGTCATG 
TGCAACCAGT GCGAGTTTTG CTTCCACCTG GATTGTCACC TCCCTTCCCT GCAGGATGTT 
CCAGGGGAGG AATGGAGTTG CTCACTCTGC CACGTGCTCC CTGATCTAAA GGAGGAAGAT 
GGAAGCCTTA GCTTGGATGG AGCAGATAGC ACTGGTGTGG TAGCTAAACT CTCACCAGCC 
AACCAGCGGA AATGTGAGCG TGTTCTGCTG GCCCTTTTCT GCCATGAACC GTGCCGCCCC 
TTGCATCAGC TGGCTACCGA CTCTACATTC TCCATGGTGA GTAGTCCCAG AATAGGAGAG 
GTTGCAGGTG TCAAGTTGGT AGCCCCACTC AATGTCTGTT GTCTCTCCCT GCAGGAGCAG 
CCTGGTGGTA CCCTAGACCT GACCTTGATT CGTGCTCGCC TCCAAGAGAA GCTGTCACCT 
CCTTATAGCT CCCCCCAGGA GTTTGCCCAG GATGTGGGCC GCATGTTCAA ACAGTTCAAT 
AAGCTGACTG AGGACAAGGC AGATGTTCAG TCCATCATCG GCCTCCAGCG CTTCTTTGAG 
ACACGAATGA ATGATGCCTT TGGTGACACC AAGTTTTCTG CTGTGCTGGT AGAACCACCA 
CCACTGAACC TTCCCAGTGC TGGCCTAAGT TCTCAGGAGC TGTCTGGTCC TGGTGATGGC 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ATRX binds to atypical chromatin domains at the 3' exons of zinc finger genes to preserve H3K9me3 enrichment.
Epigenetics11(6)398-414(2016 06)
Valle-Garc?a D,Qadeer ZA,McHugh DS,Ghiraldini FG,Chowdhury AH,Hasson D,Dyer MA,Recillas-Targa F,Bernstein E

A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype.
eLife3e02313(2014 Mar)
Serra RW,Fang M,Park SM,Hutchinson L,Green MR

Novel activity of KRAB domain that functions to reinforce nuclear localization of KRAB-containing zinc finger proteins by interacting with KAP1.
Cellular and molecular life sciences : CMLS70(20)3947-58(2013 Oct)
Wang W,Cai J,Wu Y,Hu L,Chen Z,Hu J,Chen Z,Li W,Guo M,Huang Z

The KRAB zinc finger protein RSL1 regulates sex- and tissue-specific promoter methylation and dynamic hormone-responsive chromatin configuration.
Molecular and cellular biology32(18)3732-42(2012 Sep)
Krebs CJ,Schultz DC,Robins DM

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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Trim28 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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