EDEM3 cDNA ORF clone, Bos taurus(cattle)

The following EDEM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDEM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa36323 NM_001205853.1
Latest version!
Bos taurus ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa49997 XM_010813557.2
Latest version!
Bos taurus ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa216238 NM_001205853.3
Latest version!
Bos taurus ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa93056 XM_015475272.1
Latest version!
Bos taurus ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa93057 XM_015475273.1
Latest version!
Bos taurus ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
OBa49997 XM_010813557.1 Bos taurus ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa216238 NM_001205853.2 Bos taurus ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa36323
    Clone ID Related Accession (Same CDS sequence) NM_001205853.1
    Accession Version NM_001205853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03066126.1, AAFC03066125.1 and AAFC03024421.1. On Apr 20, 2011 this sequence version replaced XM_597266.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCAT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTGAGG AGTGGAGCTA AACCCCCACT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCG
    ATTGACGCAG AAGATGGGTT GAGGTTTATG CAGGAGATGA TTGAATTGTC GAGTCAGCAA
    CAAAAAGAAC AGCAGCTCCC TCCACGAGCT GTACAAATTG TTTCCCACCC GTTTTTTGGC
    CGGGTAGTAT TAACTGCTGG CCCAGCACAG TTTGGGCCAG ACCTGTCGAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAACGGTT GTTCAGAACT TACTAACCCT
    GAAGCAGTGA TGGGAAAAAT TGCTTTGATA CAAAGAGGGC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TACAGAATGC TGGAGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGCG ATACTGCCCC TCTGTTCCAG ATGGCGGGCG ACGGGAAGGA CACAGATGAC
    ATCACGATCC CCATGCTGTT TCTATTCAGC AAAGAAGGAG GTATCATACT GGATGCCATC
    CGGGAGTATG AGGAGGTAGA AGTGCTCCTT TCTGACAAAG CAAAAGATCG AGATCCTGAG
    ATGGAAAATG AAGAACAACC ATCCTCTGAA AATGACTCTC AGAGTGCTGA ACAGATTGCA
    TCAAGGTCTC AGGAGATTGA TCTGGTTGAT CAAGAGTCTC CTGAGGAAAG TTCTGTGAAC
    TCTCACCCAG AAGCTTTATC TCCAGTAGAT ACAGACAGTG CTGCAAGCGT TTCTTCTTCT
    GAACAGGATT CTAACCCCAT GGAAAACCTC GAGACTACAA GTCTTGATGG GGAATGTACA
    GATTTAGATA ACCAACTTCA AGAACAGCCA GAAACTGAGG AGGATTCCAA TCCTAATGTT
    AGCTGGGGCA AAAAGGCCCA GCCTATAGAC TCCATATTAG CAGACTGGAA TGAAGACATA
    GAAGCATTTG AAATGATGGA TAAGGATGAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192782.1
    CDS1..2796
    Translation

    Target ORF information:

    RefSeq Version NM_001205853.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCAT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTGAGG AGTGGAGCTA AACCCCCACT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCG
    ATTGACGCAG AAGATGGGTT GAGGTTTATG CAGGAGATGA TTGAATTGTC GAGTCAGCAA
    CAAAAAGAAC AGCAGCTCCC TCCACGAGCT GTACAAATTG TTTCCCACCC GTTTTTTGGC
    CGGGTAGTAT TAACTGCTGG CCCAGCACAG TTTGGGCCAG ACCTGTCGAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAACGGTT GTTCAGAACT TACTAACCCT
    GAAGCAGTGA TGGGAAAAAT TGCTTTGATA CAAAGAGGGC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TACAGAATGC TGGAGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGCG ATACTGCCCC TCTGTTCCAG ATGGCGGGCG ACGGGAAGGA CACAGATGAC
    ATCACGATCC CCATGCTGTT TCTATTCAGC AAAGAAGGAG GTATCATACT GGATGCCATC
    CGGGAGTATG AGGAGGTAGA AGTGCTCCTT TCTGACAAAG CAAAAGATCG AGATCCTGAG
    ATGGAAAATG AAGAACAACC ATCCTCTGAA AATGACTCTC AGAGTGCTGA ACAGATTGCA
    TCAAGGTCTC AGGAGATTGA TCTGGTTGAT CAAGAGTCTC CTGAGGAAAG TTCTGTGAAC
    TCTCACCCAG AAGCTTTATC TCCAGTAGAT ACAGACAGTG CTGCAAGCGT TTCTTCTTCT
    GAACAGGATT CTAACCCCAT GGAAAACCTC GAGACTACAA GTCTTGATGG GGAATGTACA
    GATTTAGATA ACCAACTTCA AGAACAGCCA GAAACTGAGG AGGATTCCAA TCCTAATGTT
    AGCTGGGGCA AAAAGGCCCA GCCTATAGAC TCCATATTAG CAGACTGGAA TGAAGACATA
    GAAGCATTTG AAATGATGGA TAAGGATGAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49997
    Clone ID Related Accession (Same CDS sequence) XM_010813557.2 , XM_010813557.1
    Accession Version XM_010813557.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTG AGGAGTGGAG CTAAACCCCC ACTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAGCAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCGATTGACG CAGAAGATGG GTTGAGGTTT ATGCAGGAGA TGATTGAATT GTCGAGTCAG
    CAACAAAAAG AACAGCAGCT CCCTCCACGA GCTGTACAAA TTGTTTCCCA CCCGTTTTTT
    GGCCGGGTAG TATTAACTGC TGGCCCAGCA CAGTTTGGGC CAGACCTGTC GAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAACG GTTGTTCAGA ACTTACTAAC
    CCTGAAGCAG TGATGGGAAA AATTGCTTTG ATACAAAGAG GGCAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATACAGAA TGCTGGAGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GCGATACTGC CCCTCTGTTC CAGATGGCGG GCGACGGGAA GGACACAGAT
    GACATCACGA TCCCCATGCT GTTTCTATTC AGCAAAGAAG GAGGTATCAT ACTGGATGCC
    ATCCGGGAGT ATGAGGAGGT AGAAGTGCTC CTTTCTGACA AAGCAAAAGA TCGAGATCCT
    GAGATGGAAA ATGAAGAACA ACCATCCTCT GAAAATGACT CTCAGAGTGC TGAACAGATT
    GCATCAAGGT CTCAGGAGAT TGATCTGGTT GATCAAGAGT CTCCTGAGGA AAGTTCTGTG
    AACTCTCACC CAGAAGCTTT ATCTCCAGTA GATACAGACA GTGCTGCAAG CGTTTCTTCT
    TCTGAACAGG ATTCTAACCC CATGGAAAAC CTCGAGACTA CAAGTCTTGA TGGGGAATGT
    ACAGATTTAG ATAACCAACT TCAAGAACAG CCAGAAACTG AGGAGGATTC CAATCCTAAT
    GTTAGCTGGG GCAAAAAGGC CCAGCCTATA GACTCCATAT TAGCAGACTG GAATGAAGAC
    ATAGAAGCAT TTGAAATGAT GGATAAGGAT GAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811859.1
    CDS262..3060
    Translation

    Target ORF information:

    RefSeq Version XM_010813557.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813557.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTG AGGAGTGGAG CTAAACCCCC ACTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAGCAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCGATTGACG CAGAAGATGG GTTGAGGTTT ATGCAGGAGA TGATTGAATT GTCGAGTCAG
    CAACAAAAAG AACAGCAGCT CCCTCCACGA GCTGTACAAA TTGTTTCCCA CCCGTTTTTT
    GGCCGGGTAG TATTAACTGC TGGCCCAGCA CAGTTTGGGC CAGACCTGTC GAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAACG GTTGTTCAGA ACTTACTAAC
    CCTGAAGCAG TGATGGGAAA AATTGCTTTG ATACAAAGAG GGCAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATACAGAA TGCTGGAGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GCGATACTGC CCCTCTGTTC CAGATGGCGG GCGACGGGAA GGACACAGAT
    GACATCACGA TCCCCATGCT GTTTCTATTC AGCAAAGAAG GAGGTATCAT ACTGGATGCC
    ATCCGGGAGT ATGAGGAGGT AGAAGTGCTC CTTTCTGACA AAGCAAAAGA TCGAGATCCT
    GAGATGGAAA ATGAAGAACA ACCATCCTCT GAAAATGACT CTCAGAGTGC TGAACAGATT
    GCATCAAGGT CTCAGGAGAT TGATCTGGTT GATCAAGAGT CTCCTGAGGA AAGTTCTGTG
    AACTCTCACC CAGAAGCTTT ATCTCCAGTA GATACAGACA GTGCTGCAAG CGTTTCTTCT
    TCTGAACAGG ATTCTAACCC CATGGAAAAC CTCGAGACTA CAAGTCTTGA TGGGGAATGT
    ACAGATTTAG ATAACCAACT TCAAGAACAG CCAGAAACTG AGGAGGATTC CAATCCTAAT
    GTTAGCTGGG GCAAAAAGGC CCAGCCTATA GACTCCATAT TAGCAGACTG GAATGAAGAC
    ATAGAAGCAT TTGAAATGAT GGATAAGGAT GAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49997
    Clone ID Related Accession (Same CDS sequence) XM_010813557.2 , XM_010813557.1
    Accession Version XM_010813557.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTG AGGAGTGGAG CTAAACCCCC ACTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAGCAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCGATTGACG CAGAAGATGG GTTGAGGTTT ATGCAGGAGA TGATTGAATT GTCGAGTCAG
    CAACAAAAAG AACAGCAGCT CCCTCCACGA GCTGTACAAA TTGTTTCCCA CCCGTTTTTT
    GGCCGGGTAG TATTAACTGC TGGCCCAGCA CAGTTTGGGC CAGACCTGTC GAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAACG GTTGTTCAGA ACTTACTAAC
    CCTGAAGCAG TGATGGGAAA AATTGCTTTG ATACAAAGAG GGCAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATACAGAA TGCTGGAGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GCGATACTGC CCCTCTGTTC CAGATGGCGG GCGACGGGAA GGACACAGAT
    GACATCACGA TCCCCATGCT GTTTCTATTC AGCAAAGAAG GAGGTATCAT ACTGGATGCC
    ATCCGGGAGT ATGAGGAGGT AGAAGTGCTC CTTTCTGACA AAGCAAAAGA TCGAGATCCT
    GAGATGGAAA ATGAAGAACA ACCATCCTCT GAAAATGACT CTCAGAGTGC TGAACAGATT
    GCATCAAGGT CTCAGGAGAT TGATCTGGTT GATCAAGAGT CTCCTGAGGA AAGTTCTGTG
    AACTCTCACC CAGAAGCTTT ATCTCCAGTA GATACAGACA GTGCTGCAAG CGTTTCTTCT
    TCTGAACAGG ATTCTAACCC CATGGAAAAC CTCGAGACTA CAAGTCTTGA TGGGGAATGT
    ACAGATTTAG ATAACCAACT TCAAGAACAG CCAGAAACTG AGGAGGATTC CAATCCTAAT
    GTTAGCTGGG GCAAAAAGGC CCAGCCTATA GACTCCATAT TAGCAGACTG GAATGAAGAC
    ATAGAAGCAT TTGAAATGAT GGATAAGGAT GAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811859.1
    CDS94..2892
    Translation

    Target ORF information:

    RefSeq Version XM_010813557.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTG AGGAGTGGAG CTAAACCCCC ACTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAGCAT TTAGAAATCT TGAAGAAGAT GGGGGTGAGT
    TTGATTCACC TCAAAGATGG GAGAGTCCAG TTGGTTCAAC ATGCAATCCA AGCTGCTAGT
    TCGATTGACG CAGAAGATGG GTTGAGGTTT ATGCAGGAGA TGATTGAATT GTCGAGTCAG
    CAACAAAAAG AACAGCAGCT CCCTCCACGA GCTGTACAAA TTGTTTCCCA CCCGTTTTTT
    GGCCGGGTAG TATTAACTGC TGGCCCAGCA CAGTTTGGGC CAGACCTGTC GAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAACG GTTGTTCAGA ACTTACTAAC
    CCTGAAGCAG TGATGGGAAA AATTGCTTTG ATACAAAGAG GGCAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATACAGAA TGCTGGAGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GCGATACTGC CCCTCTGTTC CAGATGGCGG GCGACGGGAA GGACACAGAT
    GACATCACGA TCCCCATGCT GTTTCTATTC AGCAAAGAAG GAGGTATCAT ACTGGATGCC
    ATCCGGGAGT ATGAGGAGGT AGAAGTGCTC CTTTCTGACA AAGCAAAAGA TCGAGATCCT
    GAGATGGAAA ATGAAGAACA ACCATCCTCT GAAAATGACT CTCAGAGTGC TGAACAGATT
    GCATCAAGGT CTCAGGAGAT TGATCTGGTT GATCAAGAGT CTCCTGAGGA AAGTTCTGTG
    AACTCTCACC CAGAAGCTTT ATCTCCAGTA GATACAGACA GTGCTGCAAG CGTTTCTTCT
    TCTGAACAGG ATTCTAACCC CATGGAAAAC CTCGAGACTA CAAGTCTTGA TGGGGAATGT
    ACAGATTTAG ATAACCAACT TCAAGAACAG CCAGAAACTG AGGAGGATTC CAATCCTAAT
    GTTAGCTGGG GCAAAAAGGC CCAGCCTATA GACTCCATAT TAGCAGACTG GAATGAAGAC
    ATAGAAGCAT TTGAAATGAT GGATAAGGAT GAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216238
    Clone ID Related Accession (Same CDS sequence) NM_001205853.2 , NM_001205853.3
    Accession Version NM_001205853.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02043595.1. On Jun 30, 2017 this sequence version replaced NM_001205853.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTGAGG AGTGGAGCTA AACCCCCACT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCG
    ATTGACGCAG AAGATGGGTT GAGGTTTATG CAGGAGATGA TTGAATTGTC GAGTCAGCAA
    CAAAAAGAAC AGCAGCTCCC TCCACGAGCT GTACAAATTG TTTCCCACCC GTTTTTTGGC
    CGGGTAGTAT TAACTGCTGG CCCAGCACAG TTTGGGCCAG ACCTGTCGAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAACGGTT GTTCAGAACT TACTAACCCT
    GAAGCAGTGA TGGGAAAAAT TGCTTTGATA CAAAGAGGGC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TACAGAATGC TGGAGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGCG ATACTGCCCC TCTGTTCCAG ATGGCGGGCG ACGGGAAGGA CACAGATGAC
    ATCACGATCC CCATGCTGTT TCTATTCAGC AAAGAAGGAG GTATCATACT GGATGCCATC
    CGGGAGTATG AGGAGGTAGA AGTGCTCCTT TCTGACAAAG CAAAAGATCG AGATCCTGAG
    ATGGAAAATG AAGAACAACC ATCCTCTGAA AATGACTCTC AGAGTGCTGA ACAGATTGCA
    TCAAGGTCTC AGGAGATTGA TCTGGTTGAT CAAGAGTCTC CTGAGGAAAG TTCTGTGAAC
    TCTCACCCAG AAGCTTTATC TCCAGTAGAT ACAGACAGTG CTGCAAGCGT TTCTTCTTCT
    GAACAGGATT CTAACCCCAT GGAAAACCTC GAGACTACAA GTCTTGATGG GGAATGTACA
    GATTTAGATA ACCAACTTCA AGAACAGCCA GAAACTGAGG AGGATTCCAA TCCTAATGTT
    AGCTGGGGCA AAAAGGCCCA GCCTATAGAC TCCATATTAG CAGACTGGAA TGAAGACATA
    GAAGCATTTG AAATGATGGA TAAGGATGAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192782.2
    CDS1..2796
    Translation

    Target ORF information:

    RefSeq Version NM_001205853.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205853.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTGAGG AGTGGAGCTA AACCCCCACT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCG
    ATTGACGCAG AAGATGGGTT GAGGTTTATG CAGGAGATGA TTGAATTGTC GAGTCAGCAA
    CAAAAAGAAC AGCAGCTCCC TCCACGAGCT GTACAAATTG TTTCCCACCC GTTTTTTGGC
    CGGGTAGTAT TAACTGCTGG CCCAGCACAG TTTGGGCCAG ACCTGTCGAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAACGGTT GTTCAGAACT TACTAACCCT
    GAAGCAGTGA TGGGAAAAAT TGCTTTGATA CAAAGAGGGC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TACAGAATGC TGGAGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGCG ATACTGCCCC TCTGTTCCAG ATGGCGGGCG ACGGGAAGGA CACAGATGAC
    ATCACGATCC CCATGCTGTT TCTATTCAGC AAAGAAGGAG GTATCATACT GGATGCCATC
    CGGGAGTATG AGGAGGTAGA AGTGCTCCTT TCTGACAAAG CAAAAGATCG AGATCCTGAG
    ATGGAAAATG AAGAACAACC ATCCTCTGAA AATGACTCTC AGAGTGCTGA ACAGATTGCA
    TCAAGGTCTC AGGAGATTGA TCTGGTTGAT CAAGAGTCTC CTGAGGAAAG TTCTGTGAAC
    TCTCACCCAG AAGCTTTATC TCCAGTAGAT ACAGACAGTG CTGCAAGCGT TTCTTCTTCT
    GAACAGGATT CTAACCCCAT GGAAAACCTC GAGACTACAA GTCTTGATGG GGAATGTACA
    GATTTAGATA ACCAACTTCA AGAACAGCCA GAAACTGAGG AGGATTCCAA TCCTAATGTT
    AGCTGGGGCA AAAAGGCCCA GCCTATAGAC TCCATATTAG CAGACTGGAA TGAAGACATA
    GAAGCATTTG AAATGATGGA TAAGGATGAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216238
    Clone ID Related Accession (Same CDS sequence) NM_001205853.2 , NM_001205853.3
    Accession Version NM_001205853.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-19
    Organism Bos taurus(cattle)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000016.1. On Dec 20, 2018 this sequence version replaced NM_001205853.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571258.9346.1, SRR5571260.46024.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTGAGG AGTGGAGCTA AACCCCCACT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCG
    ATTGACGCAG AAGATGGGTT GAGGTTTATG CAGGAGATGA TTGAATTGTC GAGTCAGCAA
    CAAAAAGAAC AGCAGCTCCC TCCACGAGCT GTACAAATTG TTTCCCACCC GTTTTTTGGC
    CGGGTAGTAT TAACTGCTGG CCCAGCACAG TTTGGGCCAG ACCTGTCGAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAACGGTT GTTCAGAACT TACTAACCCT
    GAAGCAGTGA TGGGAAAAAT TGCTTTGATA CAAAGAGGGC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TACAGAATGC TGGAGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGCG ATACTGCCCC TCTGTTCCAG ATGGCGGGCG ACGGGAAGGA CACAGATGAC
    ATCACGATCC CCATGCTGTT TCTATTCAGC AAAGAAGGAG GTATCATACT GGATGCCATC
    CGGGAGTATG AGGAGGTAGA AGTGCTCCTT TCTGACAAAG CAAAAGATCG AGATCCTGAG
    ATGGAAAATG AAGAACAACC ATCCTCTGAA AATGACTCTC AGAGTGCTGA ACAGATTGCA
    TCAAGGTCTC AGGAGATTGA TCTGGTTGAT CAAGAGTCTC CTGAGGAAAG TTCTGTGAAC
    TCTCACCCAG AAGCTTTATC TCCAGTAGAT ACAGACAGTG CTGCAAGCGT TTCTTCTTCT
    GAACAGGATT CTAACCCCAT GGAAAACCTC GAGACTACAA GTCTTGATGG GGAATGTACA
    GATTTAGATA ACCAACTTCA AGAACAGCCA GAAACTGAGG AGGATTCCAA TCCTAATGTT
    AGCTGGGGCA AAAAGGCCCA GCCTATAGAC TCCATATTAG CAGACTGGAA TGAAGACATA
    GAAGCATTTG AAATGATGGA TAAGGATGAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192782.2
    CDS1..2796
    Translation

    Target ORF information:

    RefSeq Version NM_001205853.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ER degradation enhancing alpha-mannosidase like protein 3 (EDEM3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205853.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTGAGG AGTGGAGCTA AACCCCCACT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCTTGA AGAAGATGGG GGTGAGTTTG
    ATTCACCTCA AAGATGGGAG AGTCCAGTTG GTTCAACATG CAATCCAAGC TGCTAGTTCG
    ATTGACGCAG AAGATGGGTT GAGGTTTATG CAGGAGATGA TTGAATTGTC GAGTCAGCAA
    CAAAAAGAAC AGCAGCTCCC TCCACGAGCT GTACAAATTG TTTCCCACCC GTTTTTTGGC
    CGGGTAGTAT TAACTGCTGG CCCAGCACAG TTTGGGCCAG ACCTGTCGAA ACATAAAGAG
    ACAAGAGGAT TTGTTGCAAG CAGTAAACCA TACAACGGTT GTTCAGAACT TACTAACCCT
    GAAGCAGTGA TGGGAAAAAT TGCTTTGATA CAAAGAGGGC AGTGCATGTT TGCAGAAAAG
    GCACGCAACA TACAGAATGC TGGAGCCATT GGTGGCATTG TTATTGATGA CAATGAGGGG
    AGCAGCAGCG ATACTGCCCC TCTGTTCCAG ATGGCGGGCG ACGGGAAGGA CACAGATGAC
    ATCACGATCC CCATGCTGTT TCTATTCAGC AAAGAAGGAG GTATCATACT GGATGCCATC
    CGGGAGTATG AGGAGGTAGA AGTGCTCCTT TCTGACAAAG CAAAAGATCG AGATCCTGAG
    ATGGAAAATG AAGAACAACC ATCCTCTGAA AATGACTCTC AGAGTGCTGA ACAGATTGCA
    TCAAGGTCTC AGGAGATTGA TCTGGTTGAT CAAGAGTCTC CTGAGGAAAG TTCTGTGAAC
    TCTCACCCAG AAGCTTTATC TCCAGTAGAT ACAGACAGTG CTGCAAGCGT TTCTTCTTCT
    GAACAGGATT CTAACCCCAT GGAAAACCTC GAGACTACAA GTCTTGATGG GGAATGTACA
    GATTTAGATA ACCAACTTCA AGAACAGCCA GAAACTGAGG AGGATTCCAA TCCTAATGTT
    AGCTGGGGCA AAAAGGCCCA GCCTATAGAC TCCATATTAG CAGACTGGAA TGAAGACATA
    GAAGCATTTG AAATGATGGA TAAGGATGAG CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93056
    Clone ID Related Accession (Same CDS sequence) XM_015475272.1
    Accession Version XM_015475272.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTG AGGAGTGGAG CTAAACCCCC ACTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAGCAT TTAGAAATCT TGAAGAAGAT GGGGGCTGCT
    AGTTCGATTG ACGCAGAAGA TGGGTTGAGG TTTATGCAGG AGATGATTGA ATTGTCGAGT
    CAGCAACAAA AAGAACAGCA GCTCCCTCCA CGAGCTGTAC AAATTGTTTC CCACCCGTTT
    TTTGGCCGGG TAGTATTAAC TGCTGGCCCA GCACAGTTTG GGCCAGACCT GTCGAAACAT
    AAAGAGACAA GAGGATTTGT TGCAAGCAGT AAACCATACA ACGGTTGTTC AGAACTTACT
    AACCCTGAAG CAGTGATGGG AAAAATTGCT TTGATACAAA GAGGGCAGTG CATGTTTGCA
    GAAAAGGCAC GCAACATACA GAATGCTGGA GCCATTGGTG GCATTGTTAT TGATGACAAT
    GAGGGGAGCA GCAGCGATAC TGCCCCTCTG TTCCAGATGG CGGGCGACGG GAAGGACACA
    GATGACATCA CGATCCCCAT GCTGTTTCTA TTCAGCAAAG AAGGAGGTAT CATACTGGAT
    GCCATCCGGG AGTATGAGGA GGTAGAAGTG CTCCTTTCTG ACAAAGCAAA AGATCGAGAT
    CCTGAGATGG AAAATGAAGA ACAACCATCC TCTGAAAATG ACTCTCAGAG TGCTGAACAG
    ATTGCATCAA GGTCTCAGGA GATTGATCTG GTTGATCAAG AGTCTCCTGA GGAAAGTTCT
    GTGAACTCTC ACCCAGAAGC TTTATCTCCA GTAGATACAG ACAGTGCTGC AAGCGTTTCT
    TCTTCTGAAC AGGATTCTAA CCCCATGGAA AACCTCGAGA CTACAAGTCT TGATGGGGAA
    TGTACAGATT TAGATAACCA ACTTCAAGAA CAGCCAGAAA CTGAGGAGGA TTCCAATCCT
    AATGTTAGCT GGGGCAAAAA GGCCCAGCCT ATAGACTCCA TATTAGCAGA CTGGAATGAA
    GACATAGAAG CATTTGAAAT GATGGATAAG GATGAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330758.1
    CDS94..2835
    Translation

    Target ORF information:

    RefSeq Version XM_015475272.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475272.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTCAGAGA GGAGAGTTTG AGGAGTGGAG CTAAACCCCC ACTGAGAGCC
    AGAGATTTCA TGGCCACTAA CCCTGAGCAT TTAGAAATCT TGAAGAAGAT GGGGGCTGCT
    AGTTCGATTG ACGCAGAAGA TGGGTTGAGG TTTATGCAGG AGATGATTGA ATTGTCGAGT
    CAGCAACAAA AAGAACAGCA GCTCCCTCCA CGAGCTGTAC AAATTGTTTC CCACCCGTTT
    TTTGGCCGGG TAGTATTAAC TGCTGGCCCA GCACAGTTTG GGCCAGACCT GTCGAAACAT
    AAAGAGACAA GAGGATTTGT TGCAAGCAGT AAACCATACA ACGGTTGTTC AGAACTTACT
    AACCCTGAAG CAGTGATGGG AAAAATTGCT TTGATACAAA GAGGGCAGTG CATGTTTGCA
    GAAAAGGCAC GCAACATACA GAATGCTGGA GCCATTGGTG GCATTGTTAT TGATGACAAT
    GAGGGGAGCA GCAGCGATAC TGCCCCTCTG TTCCAGATGG CGGGCGACGG GAAGGACACA
    GATGACATCA CGATCCCCAT GCTGTTTCTA TTCAGCAAAG AAGGAGGTAT CATACTGGAT
    GCCATCCGGG AGTATGAGGA GGTAGAAGTG CTCCTTTCTG ACAAAGCAAA AGATCGAGAT
    CCTGAGATGG AAAATGAAGA ACAACCATCC TCTGAAAATG ACTCTCAGAG TGCTGAACAG
    ATTGCATCAA GGTCTCAGGA GATTGATCTG GTTGATCAAG AGTCTCCTGA GGAAAGTTCT
    GTGAACTCTC ACCCAGAAGC TTTATCTCCA GTAGATACAG ACAGTGCTGC AAGCGTTTCT
    TCTTCTGAAC AGGATTCTAA CCCCATGGAA AACCTCGAGA CTACAAGTCT TGATGGGGAA
    TGTACAGATT TAGATAACCA ACTTCAAGAA CAGCCAGAAA CTGAGGAGGA TTCCAATCCT
    AATGTTAGCT GGGGCAAAAA GGCCCAGCCT ATAGACTCCA TATTAGCAGA CTGGAATGAA
    GACATAGAAG CATTTGAAAT GATGGATAAG GATGAGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93057
    Clone ID Related Accession (Same CDS sequence) XM_015475273.1
    Accession Version XM_015475273.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ER degradation-enhancing alpha-mannosidase-like protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTGAGG AGTGGAGCTA AACCCCCACT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCTTGA AGAAGATGGG GGCTGCTAGT
    TCGATTGACG CAGAAGATGG GTTGAGGTTT ATGCAGGAGA TGATTGAATT GTCGAGTCAG
    CAACAAAAAG AACAGCAGCT CCCTCCACGA GCTGTACAAA TTGTTTCCCA CCCGTTTTTT
    GGCCGGGTAG TATTAACTGC TGGCCCAGCA CAGTTTGGGC CAGACCTGTC GAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAACG GTTGTTCAGA ACTTACTAAC
    CCTGAAGCAG TGATGGGAAA AATTGCTTTG ATACAAAGAG GGCAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATACAGAA TGCTGGAGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GCGATACTGC CCCTCTGTTC CAGATGGCGG GCGACGGGAA GGACACAGAT
    GACATCACGA TCCCCATGCT GTTTCTATTC AGCAAAGAAG GAGGTATCAT ACTGGATGCC
    ATCCGGGAGT ATGAGGAGGT AGAAGTGCTC CTTTCTGACA AAGCAAAAGA TCGAGATCCT
    GAGATGGAAA ATGAAGAACA ACCATCCTCT GAAAATGACT CTCAGAGTGC TGAACAGATT
    GCATCAAGGT CTCAGGAGAT TGATCTGGTT GATCAAGAGT CTCCTGAGGA AAGTTCTGTG
    AACTCTCACC CAGAAGCTTT ATCTCCAGTA GATACAGACA GTGCTGCAAG CGTTTCTTCT
    TCTGAACAGG ATTCTAACCC CATGGAAAAC CTCGAGACTA CAAGTCTTGA TGGGGAATGT
    ACAGATTTAG ATAACCAACT TCAAGAACAG CCAGAAACTG AGGAGGATTC CAATCCTAAT
    GTTAGCTGGG GCAAAAAGGC CCAGCCTATA GACTCCATAT TAGCAGACTG GAATGAAGAC
    ATAGAAGCAT TTGAAATGAT GGATAAGGAT GAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330759.1
    CDS94..2832
    Translation

    Target ORF information:

    RefSeq Version XM_015475273.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ER degradation enhancer, mannosidase alpha-like 3 (EDEM3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475273.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGCGAAG CCGGCGGCGG CCGGGGCTCT GGGTCCTCGG TCCCCCAGCG AGCGCCATGG 
    AGCCTGGTGG CGGCGACGGC CGCGCTGTGC CTGGTGTCGG CCACGTCCGT GTGGACGGCG
    GGGGCCGCGC CCATGAGTCG CGAGGAGAAG CAGAAGCTTG GGAATCAAGT ACTGGAAATG
    TTTGATCATG CTTATGGTAA CTATATGGAA CATGCTTACC CAGCGGATGA ACTCATGCCT
    TTGACCTGCA GAGGTCGCGT TAGAGGCCAG GAGCCAAGTC GGGGTGATGT TGATGATGCC
    TTGGGAAAAT TTTCCCTGAC ACTGATTGAT TCTTTGGATA CTCTTGTGGT ATTAAATAAA
    ACTAAAGAAT TTGAAGATGC AGTGAGAAAA GTTTTAAGAG ATGTTAACTT AGATAATGAT
    GTAGTTGTAT CAGTCTTTGA AACAAACATC AGAGTTCTTG GGGGTCTTTT GGGTGGACAC
    TCACTGGCAA TCATGCTGAA AGAAAAAGGC GAGTACATGC AGTGGTACAA TGATGAGCTT
    CTCCAAATGG CAAAGCAATT GGGCTACAAA CTTTTACCAG CTTTCAATAC TACCAGTGGC
    CTTCCTTATC CAAGAATCAA TTTGAAGTTT GGTATCAGAA AACCAGAAGC TCGGACAGGT
    ACTGAGACTG ATACCTGTAC AGCCTGTGCA GGCACCTTGA TCCTTGAGTT TGCTGCTCTA
    AGTCGATTCA CAGGAGCGAC GATATTTGAG GAATATGCAA GAAAAGCTCT TGATTTTCTC
    TGGGAGAAAA GACAGAGAAG TAGTAATTTA GTGGGTGTGA CTATAAATAT TCATACTGGA
    GACTGGGTAC GAAAAGATAG TGGAGTTGGA GCAGGGATTG ATTCATATTA TGAGTACCTA
    TTGAAAGCCT ATGTCTTGCT TGGAGATGAC AGCTTTCTGG AAAGATTTAA TACACACTAT
    GATGCCATCA TGAGGTATAT AAGCCAGCCA CCTCTTCTAC TTGATGTGCA TATCCACAAG
    CCAATGCTGA ATGCTCGGAC TTGGATGGAT GCTTTGCTTG CCTTTTTTCC AGGTTTGCAG
    GTCTTAAAGG GAGATATTAG ACCTGCTATT GAAACTCATG AAATGTTATA TCAGGTGATT
    AAAAAACACA ATTTTCTACC AGAGGCATTT ACCACAGATT TCAGGGTACA TTGGGCTCAA
    CATCCGTTAA GGCCAGAATT TGCAGAAAGT ACCTACTTCT TGTATAAAGC TACAGGAGAT
    CCTTACTACC TTGAAGTAGG GAAAACACTT ATTGAAAATT TAAATAAATA TGCTAGAGTG
    CCTTGCGGAT TTGCTGCCAT GAAGGATGTT CGTACTGGAA GTCATGAGGA CAGAATGGAT
    TCCTTCTTCT TGGCGGAAAT GTTTAAATAT CTTTACCTGT TATTTGCGGA TAAAGAAGAC
    ATTATTTTTG ACATAGAAGA TTACATTTTT ACAACAGAAG CTCATCTGTT ACCTCTTTGG
    CTCTCTACTA CAAATCAAAG AGTCTCTAAG AAGAACACAA CTTCAGAATA TACAGAACTG
    GATGACAGTA ATTTTGACTG GACTTGTCCA AACACTCAGA TCCTCTTCCC TAATGATCCA
    CTATATGCTC AGAGCATCCG TGAACCCTTG AAAAATGTGG TGGACAAGAG CTGTCCTAGA
    GGCATCATCA GAGTAGAGGA GAGTTTGAGG AGTGGAGCTA AACCCCCACT GAGAGCCAGA
    GATTTCATGG CCACTAACCC TGAGCATTTA GAAATCTTGA AGAAGATGGG GGCTGCTAGT
    TCGATTGACG CAGAAGATGG GTTGAGGTTT ATGCAGGAGA TGATTGAATT GTCGAGTCAG
    CAACAAAAAG AACAGCAGCT CCCTCCACGA GCTGTACAAA TTGTTTCCCA CCCGTTTTTT
    GGCCGGGTAG TATTAACTGC TGGCCCAGCA CAGTTTGGGC CAGACCTGTC GAAACATAAA
    GAGACAAGAG GATTTGTTGC AAGCAGTAAA CCATACAACG GTTGTTCAGA ACTTACTAAC
    CCTGAAGCAG TGATGGGAAA AATTGCTTTG ATACAAAGAG GGCAGTGCAT GTTTGCAGAA
    AAGGCACGCA ACATACAGAA TGCTGGAGCC ATTGGTGGCA TTGTTATTGA TGACAATGAG
    GGGAGCAGCA GCGATACTGC CCCTCTGTTC CAGATGGCGG GCGACGGGAA GGACACAGAT
    GACATCACGA TCCCCATGCT GTTTCTATTC AGCAAAGAAG GAGGTATCAT ACTGGATGCC
    ATCCGGGAGT ATGAGGAGGT AGAAGTGCTC CTTTCTGACA AAGCAAAAGA TCGAGATCCT
    GAGATGGAAA ATGAAGAACA ACCATCCTCT GAAAATGACT CTCAGAGTGC TGAACAGATT
    GCATCAAGGT CTCAGGAGAT TGATCTGGTT GATCAAGAGT CTCCTGAGGA AAGTTCTGTG
    AACTCTCACC CAGAAGCTTT ATCTCCAGTA GATACAGACA GTGCTGCAAG CGTTTCTTCT
    TCTGAACAGG ATTCTAACCC CATGGAAAAC CTCGAGACTA CAAGTCTTGA TGGGGAATGT
    ACAGATTTAG ATAACCAACT TCAAGAACAG CCAGAAACTG AGGAGGATTC CAATCCTAAT
    GTTAGCTGGG GCAAAAAGGC CCAGCCTATA GACTCCATAT TAGCAGACTG GAATGAAGAC
    ATAGAAGCAT TTGAAATGAT GGATAAGGAT GAGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.