EPHB4 cDNA ORF clone, Bos taurus(cattle)

The following EPHB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPHB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238987 NM_001206268.3
Latest version!
Bos taurus EPH receptor B4 (EPHB4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa230266 XM_010819549.3
Latest version!
Bos taurus EPH receptor B4 (EPHB4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa216163 NM_001206268.2
Latest version!
Bos taurus EPH receptor B4 (EPHB4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa37585 NM_001206268.1
Latest version!
Bos taurus EPH receptor B4 (EPHB4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa49407 XM_010819549.2
Latest version!
Bos taurus EPH receptor B4 (EPHB4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238987
    Clone ID Related Accession (Same CDS sequence) NM_001206268.3
    Accession Version NM_001206268.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product ephrin type-B receptor 4 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000025.1. On May 29, 2018 this sequence version replaced NM_001206268.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCTCC GGGCTCTGCT CTGCTGGGCT TCGCTTGCAG CCGCCCTAGA AGAGACCCTA 
    CTGAACACGA AATTGGAAAC TGCCGATCTG AAGTGGGTGA CATTCCCTCA GGCGGACGGG
    CAGTGGGAGG AGCTGAGCGG CCTGGACGAG GAGCAGCACA GCGTCCGGAC CTACGAGGTG
    TGTGACATGC AGCGGGCCCC AGGCCTGGCC CACTGGCTGC GCACCGGCTG GGTCCCCCGC
    CGCGGGGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTGGAGTG CCTCTCCCTG
    CCGAGGGCCG GCCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTTCGA GAGCGACGCA
    GACACGGCCA CGGCCCACAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
    GTGGCTGCTG AGCACCTCAC CCGGAAGCGC CCAGGGGCTG AGGCCACGGG GAAGGTGAAT
    GTCAAGACGC TGCGTCTGGG CCCGCTCACC AAGGCCGGCT TCTACCTGGC CTTCCAGGAC
    CAGGGTGCCT GCATGGCCCT GCTGTCCCTG CACCTCTTCT ATAAGAAGTG CGCCCAGCAG
    ACTGTGAACC TCACCTACTT CCCGGAGACT GTGCCTCGGG AGCTGGTGGT GCCCGTGGCC
    GGCAGCTGCG TGGCCGATGC CATGCCTGCC CCCGGCCCCA GCCCCAGCCT CTACTGCCGG
    GAAGACGGCC AGTGGGCCGA GCAGCCGGTC ACGGGCTGCA GCTGCAATGC AGGGTTCGAG
    GCCGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCTCAGG GCACCTTCAA GCCCCTGTCC
    GGGGAGGGGT CCTGCCAGCC GTGCCCAGCC AACAGCCACT CTAACGCCAT TGGCTCGTCC
    ATCTGCCAGT GTCGCATCGG GTACTTCCGG GCCAGCACAG ACCCCCGAAG TGCGCCCTGT
    ACCACACCCC CGTCAGCCCC GAGAAGCGTG GTTCCCCGCC TGAATGGCTC CGCCCTGCGC
    TTGGAGTGGA GTGCGCCCCT CGAGTCAGGC GGCCGGGAGG ACCTCACTTA CGCGCTCCGC
    TGCCGGGAGT GCCGGCCCGG TGGGTCCTGC ACGCCCTGCG GGGGAGACCT GACCTTCGAC
    CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGCGATTC GCGGTCTGCG TCCTGACGTC
    ACCTATACGT TTGAGGTCAC CGCCTTGAAT GGGGTGTCCT CCTTGGCCAG TGGGCCGGTC
    CCGTTTGAGG CTGTGAATGT CACCACGGAC CGCGAGGTGC CACCCCCCGT GTCTGACATC
    CGGGTGACTC GGTCATCACC CAGCAGCTTG AGCCTGGCAT GGGCCGTTCC CCGGGCGCCC
    AGCGGGGCAG TGCTGGACTA CGAGGTCAAG TACCACGAGA AGGGCGCTGA GGGCCCCAGC
    AGCGTGCGGT TCCTGAAGAC GTCAGAGAAC CGGGCAGAGC TGCGGGGACT GAAGCGGGGA
    GCCAGCTACC TGGTCCAGGT GCGGGCACGC TCCGAGGCCG GCTACGGGCC CTTTGGCCAG
    GAGCACCACA GCCAGACCCA GCTGGACGAG AATGAGGCCT GGAGGGAGCA GCTGGCCCTG
    ATTGCAGGCA CCGCGGCCGT GGGTGTGGTG CTAGTGTTCG TGGTCATCGT CATCGCAGTT
    CTCTGCCTCA GGAAGCAGAG CAACGGGAGA GAAGCTGAGT ACTCAGACAA ACACGCCCAG
    TATCTCATTG GGCACGGTAC TAAGGTCTAC ATTGACCCCT TCACTTACGA AGACCCTAAT
    GAGGCTGTCA GGGAGTTTGC AAAAGAGATC GATGTCTCCT ATGTCAAGAT TGAAGAGGTG
    ATTGGTGCAG GCGAGTTTGG TGAGGTGTGC CGGGGGCGCC TCAAGGCCCC TGGGAAGAAG
    GAGAGCTGCG TAGCCATCAA GACCCTGAAG GGCGGCTACA CGGAGCGGCA GCGGCGGGAG
    TTCCTGAGTG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG
    GGCGTGGTCA CCAACAGCGT GCCCGTCATG ATCCTCACCG AGTTCATGGA GAACGGTGCC
    CTGGACTCCT TCCTGCGGCT GAACGACGGC CAGTTCACCG TGATCCAGCT CGTGGGCATG
    CTACGGGGCA TCGCCTCGGG CATGCGCTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC
    CTGGCTGCCC GCAACATCCT GGTCAACAGC AACCTCGTCT GCAAGGTCTC AGACTTTGGC
    CTCTCCCGAT TCCTGGAGGA GAACTCCTCC GACCCCACCT ACACGAGCTC TCTGGGAGGA
    AAGATTCCCA TCCGCTGGAC AGCCCCCGAG GCCATTGCCT TCCGGAAGTT CACATCTGCC
    AGTGATGCCT GGAGCTACGG AATTGTCATG TGGGAGGTCA TGTCATTTGG GGAGCGGCCA
    TACTGGGATA TGAGCAATCA GGATGTGATC AACGCCATTG AACAGGACTA CCGGTTGCCC
    CCGCCCCCAG ACTGCCCCAC CTCCCTGCAT CAGCTCATGC TGGACTGTTG GCAGAAAGAC
    CGGAATGCGC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TCGACAAGAT GATCCGGAAC
    CCTGCCAGCC TCAAAATCGT GGCCCGGGAG AACGGCGGGG CCTCGCACCC CCTCCTGGAT
    CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCCGTGGGGG AGTGGCTTCG AGCCATTAAG
    ATGGGAAGAT ACGAAGAAAG CTTTGCAGCT GCTGGGTTTG GCTCCTTTGA GCTGGTCAGC
    CAGATCTCCA CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA
    ATCCTGGCCA GTGTCCAGCA CATGAAATCC CAGGCCAAGC CTGGAGCCCC AGGCGGGTCG
    GGAGCACCGG CCCCCCAGTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193197.3
    CDS1..2964
    Translation

    Target ORF information:

    RefSeq Version NM_001206268.3
    Organism Bos taurus(cattle)
    Definition Bos taurus EPH receptor B4 (EPHB4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206268.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCTCC GGGCTCTGCT CTGCTGGGCT TCGCTTGCAG CCGCCCTAGA AGAGACCCTA 
    CTGAACACGA AATTGGAAAC TGCCGATCTG AAGTGGGTGA CATTCCCTCA GGCGGACGGG
    CAGTGGGAGG AGCTGAGCGG CCTGGACGAG GAGCAGCACA GCGTCCGGAC CTACGAGGTG
    TGTGACATGC AGCGGGCCCC AGGCCTGGCC CACTGGCTGC GCACCGGCTG GGTCCCCCGC
    CGCGGGGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTGGAGTG CCTCTCCCTG
    CCGAGGGCCG GCCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTTCGA GAGCGACGCA
    GACACGGCCA CGGCCCACAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
    GTGGCTGCTG AGCACCTCAC CCGGAAGCGC CCAGGGGCTG AGGCCACGGG GAAGGTGAAT
    GTCAAGACGC TGCGTCTGGG CCCGCTCACC AAGGCCGGCT TCTACCTGGC CTTCCAGGAC
    CAGGGTGCCT GCATGGCCCT GCTGTCCCTG CACCTCTTCT ATAAGAAGTG CGCCCAGCAG
    ACTGTGAACC TCACCTACTT CCCGGAGACT GTGCCTCGGG AGCTGGTGGT GCCCGTGGCC
    GGCAGCTGCG TGGCCGATGC CATGCCTGCC CCCGGCCCCA GCCCCAGCCT CTACTGCCGG
    GAAGACGGCC AGTGGGCCGA GCAGCCGGTC ACGGGCTGCA GCTGCAATGC AGGGTTCGAG
    GCCGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCTCAGG GCACCTTCAA GCCCCTGTCC
    GGGGAGGGGT CCTGCCAGCC GTGCCCAGCC AACAGCCACT CTAACGCCAT TGGCTCGTCC
    ATCTGCCAGT GTCGCATCGG GTACTTCCGG GCCAGCACAG ACCCCCGAAG TGCGCCCTGT
    ACCACACCCC CGTCAGCCCC GAGAAGCGTG GTTCCCCGCC TGAATGGCTC CGCCCTGCGC
    TTGGAGTGGA GTGCGCCCCT CGAGTCAGGC GGCCGGGAGG ACCTCACTTA CGCGCTCCGC
    TGCCGGGAGT GCCGGCCCGG TGGGTCCTGC ACGCCCTGCG GGGGAGACCT GACCTTCGAC
    CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGCGATTC GCGGTCTGCG TCCTGACGTC
    ACCTATACGT TTGAGGTCAC CGCCTTGAAT GGGGTGTCCT CCTTGGCCAG TGGGCCGGTC
    CCGTTTGAGG CTGTGAATGT CACCACGGAC CGCGAGGTGC CACCCCCCGT GTCTGACATC
    CGGGTGACTC GGTCATCACC CAGCAGCTTG AGCCTGGCAT GGGCCGTTCC CCGGGCGCCC
    AGCGGGGCAG TGCTGGACTA CGAGGTCAAG TACCACGAGA AGGGCGCTGA GGGCCCCAGC
    AGCGTGCGGT TCCTGAAGAC GTCAGAGAAC CGGGCAGAGC TGCGGGGACT GAAGCGGGGA
    GCCAGCTACC TGGTCCAGGT GCGGGCACGC TCCGAGGCCG GCTACGGGCC CTTTGGCCAG
    GAGCACCACA GCCAGACCCA GCTGGACGAG AATGAGGCCT GGAGGGAGCA GCTGGCCCTG
    ATTGCAGGCA CCGCGGCCGT GGGTGTGGTG CTAGTGTTCG TGGTCATCGT CATCGCAGTT
    CTCTGCCTCA GGAAGCAGAG CAACGGGAGA GAAGCTGAGT ACTCAGACAA ACACGCCCAG
    TATCTCATTG GGCACGGTAC TAAGGTCTAC ATTGACCCCT TCACTTACGA AGACCCTAAT
    GAGGCTGTCA GGGAGTTTGC AAAAGAGATC GATGTCTCCT ATGTCAAGAT TGAAGAGGTG
    ATTGGTGCAG GCGAGTTTGG TGAGGTGTGC CGGGGGCGCC TCAAGGCCCC TGGGAAGAAG
    GAGAGCTGCG TAGCCATCAA GACCCTGAAG GGCGGCTACA CGGAGCGGCA GCGGCGGGAG
    TTCCTGAGTG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG
    GGCGTGGTCA CCAACAGCGT GCCCGTCATG ATCCTCACCG AGTTCATGGA GAACGGTGCC
    CTGGACTCCT TCCTGCGGCT GAACGACGGC CAGTTCACCG TGATCCAGCT CGTGGGCATG
    CTACGGGGCA TCGCCTCGGG CATGCGCTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC
    CTGGCTGCCC GCAACATCCT GGTCAACAGC AACCTCGTCT GCAAGGTCTC AGACTTTGGC
    CTCTCCCGAT TCCTGGAGGA GAACTCCTCC GACCCCACCT ACACGAGCTC TCTGGGAGGA
    AAGATTCCCA TCCGCTGGAC AGCCCCCGAG GCCATTGCCT TCCGGAAGTT CACATCTGCC
    AGTGATGCCT GGAGCTACGG AATTGTCATG TGGGAGGTCA TGTCATTTGG GGAGCGGCCA
    TACTGGGATA TGAGCAATCA GGATGTGATC AACGCCATTG AACAGGACTA CCGGTTGCCC
    CCGCCCCCAG ACTGCCCCAC CTCCCTGCAT CAGCTCATGC TGGACTGTTG GCAGAAAGAC
    CGGAATGCGC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TCGACAAGAT GATCCGGAAC
    CCTGCCAGCC TCAAAATCGT GGCCCGGGAG AACGGCGGGG CCTCGCACCC CCTCCTGGAT
    CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCCGTGGGGG AGTGGCTTCG AGCCATTAAG
    ATGGGAAGAT ACGAAGAAAG CTTTGCAGCT GCTGGGTTTG GCTCCTTTGA GCTGGTCAGC
    CAGATCTCCA CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA
    ATCCTGGCCA GTGTCCAGCA CATGAAATCC CAGGCCAAGC CTGGAGCCCC AGGCGGGTCG
    GGAGCACCGG CCCCCCAGTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230266
    Clone ID Related Accession (Same CDS sequence) XM_010819549.3
    Accession Version XM_010819549.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ephrin type-B receptor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819549.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCTCC GGGCTCTGCT CTGCTGGGCT TCGCTTGCAG CCGCCCTAGA AGAGACCCTA 
    CTGAACACGA AATTGGAAAC TGCCGATCTG AAGTGGGTGA CATTCCCTCA GGCGGACGGG
    CAGTGGGAGG AGCTGAGCGG CCTGGACGAG GAGCAGCACA GCGTCCGGAC CTACGAGGTG
    TGTGACATGC AGCGGGCCCC AGGCCTGGCC CACTGGCTGC GCACCGGCTG GGTCCCCCGC
    CGCGGGGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTGGAGTG CCTCTCCCTG
    CCGAGGGCCG GCCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTTCGA GAGCGACGCA
    GACACGGCCA CGGCCCACAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
    GTGGCTGCTG AGCACCTCAC CCGGAAGCGC CCAGGGGCTG AGGCCACGGG GAAGGTGAAT
    GTCAAGACGC TGCGTCTGGG CCCGCTCACC AAGGCCGGCT TCTACCTGGC CTTCCAGGAC
    CAGGGTGCCT GCATGGCCCT GCTGTCCCTG CACCTCTTCT ATAAGAAGTG CGCCCAGCAG
    ACTGTGAACC TCACCTACTT CCCGGAGACT GTGCCTCGGG AGCTGGTGGT GCCCGTGGCC
    GGCAGCTGCG TGGCCGATGC CATGCCTGCC CCCGGCCCCA GCCCCAGCCT CTACTGCCGG
    GAAGACGGCC AGTGGGCCGA GCAGCCGGTC ACGGGCTGCA GCTGCAATGC AGGGTTCGAG
    GCCGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCTCAGG GCACCTTCAA GCCCCTGTCC
    GGGGAGGGGT CCTGCCAGCC GTGCCCAGCC AACAGCCACT CTAACGCCAT TGGCTCGTCC
    ATCTGCCAGT GTCGCATCGG GTACTTCCGG GCCAGCACAG ACCCCCGAAG TGCGCCCTGT
    ACCACACCCC CGTCAGCCCC GAGAAGCGTG GTTCCCCGCC TGAATGGCTC CGCCCTGCGC
    TTGGAGTGGA GTGCGCCCCT CGAGTCAGGC GGCCGGGAGG ACCTCACTTA CGCGCTCCGC
    TGCCGGGAGT GCCGGCCCGG TGGGTCCTGC ACGCCCTGCG GGGGAGACCT GACCTTCGAC
    CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGCGATTC GCGGTCTGCG TCCTGACGTC
    ACCTATACGT TTGAGGTCAC CGCCTTGAAT GGGGTGTCCT CCTTGGCCAG TGGGCCGGTC
    CCGTTTGAGG CTGTGAATGT CACCACGGAC CGCGAGGTGC CACCCCCCGT GTCTGACATC
    CGGGTGACTC GGTCATCACC CAGCAGCTTG AGCCTGGCAT GGGCCGTTCC CCGGGCGCCC
    AGCGGGGCAG TGCTGGACTA CGAGGTCAAG TACCACGAGA AGCCCCTCTC TCCTCCGGAA
    CAGGGCGCTG AGGGCCCCAG CAGCGTGCGG TTCCTGAAGA CGTCAGAGAA CCGGGCAGAG
    CTGCGGGGAC TGAAGCGGGG AGCCAGCTAC CTGGTCCAGG TGCGGGCACG CTCCGAGGCC
    GGCTACGGGC CCTTTGGCCA GGAGCACCAC AGCCAGACCC AGCTGGACGA GAATGAGGCC
    TGGAGGGAGC AGCTGGCCCT GATTGCAGGC ACCGCGGCCG TGGGTGTGGT GCTAGTGTTC
    GTGGTCATCG TCATCGCAGT TCTCTGCCTC AGGAAGCAGA GCAACGGGAG AGAAGCTGAG
    TACTCAGACA AACACGCCCA GTATCTCATT GGGCACGGTA CTAAGGTCTA CATTGACCCC
    TTCACTTACG AAGACCCTAA TGAGGCTGTC AGGGAGTTTG CAAAAGAGAT CGATGTCTCC
    TATGTCAAGA TTGAAGAGGT GATTGGTGCA GGCGAGTTTG GTGAGGTGTG CCGGGGGCGC
    CTCAAGGCCC CTGGGAAGAA GGAGAGCTGC GTAGCCATCA AGACCCTGAA GGGCGGCTAC
    ACGGAGCGGC AGCGGCGGGA GTTCCTGAGT GAGGCCTCCA TCATGGGCCA GTTCGAGCAC
    CCCAATATCA TCCGCCTGGA GGGCGTGGTC ACCAACAGCG TGCCCGTCAT GATCCTCACC
    GAGTTCATGG AGAACGGTGC CCTGGACTCC TTCCTGCGGC TGAACGACGG CCAGTTCACC
    GTGATCCAGC TCGTGGGCAT GCTACGGGGC ATCGCCTCGG GCATGCGCTA CCTTGCCGAG
    ATGAGCTACG TCCACCGAGA CCTGGCTGCC CGCAACATCC TGGTCAACAG CAACCTCGTC
    TGCAAGGTCT CAGACTTTGG CCTCTCCCGA TTCCTGGAGG AGAACTCCTC CGACCCCACC
    TACACGAGCT CTCTGGGAGG AAAGATTCCC ATCCGCTGGA CAGCCCCCGA GGCCATTGCC
    TTCCGGAAGT TCACATCTGC CAGTGATGCC TGGAGCTACG GAATTGTCAT GTGGGAGGTC
    ATGTCATTTG GGGAGCGGCC ATACTGGGAT ATGAGCAATC AGGATGTGAT CAACGCCATT
    GAACAGGACT ACCGGTTGCC CCCGCCCCCA GACTGCCCCA CCTCCCTGCA TCAGCTCATG
    CTGGACTGTT GGCAGAAAGA CCGGAATGCG CGGCCCCGCT TCCCCCAGGT GGTCAGCGCC
    CTCGACAAGA TGATCCGGAA CCCTGCCAGC CTCAAAATCG TGGCCCGGGA GAACGGCGGG
    GCCTCGCACC CCCTCCTGGA TCAGCGGCAG CCTCACTACT CAGCTTTTGG CTCCGTGGGG
    GAGTGGCTTC GAGCCATTAA GATGGGAAGA TACGAAGAAA GCTTTGCAGC TGCTGGGTTT
    GGCTCCTTTG AGCTGGTCAG CCAGATCTCC ACTGAGGACC TGCTCCGAAT CGGAGTCACT
    CTGGCGGGAC ACCAGAAGAA AATCCTGGCC AGTGTCCAGC ACATGAAATC CCAGGCCAAG
    CCTGGAGCCC CAGGCGGGTC GGGAGCACCG GCCCCCCAGT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817851.2
    CDS383..3367
    Translation

    Target ORF information:

    RefSeq Version XM_010819549.3
    Organism Bos taurus(cattle)
    Definition Bos taurus EPH receptor B4 (EPHB4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819549.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCTCC GGGCTCTGCT CTGCTGGGCT TCGCTTGCAG CCGCCCTAGA AGAGACCCTA 
    CTGAACACGA AATTGGAAAC TGCCGATCTG AAGTGGGTGA CATTCCCTCA GGCGGACGGG
    CAGTGGGAGG AGCTGAGCGG CCTGGACGAG GAGCAGCACA GCGTCCGGAC CTACGAGGTG
    TGTGACATGC AGCGGGCCCC AGGCCTGGCC CACTGGCTGC GCACCGGCTG GGTCCCCCGC
    CGCGGGGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTGGAGTG CCTCTCCCTG
    CCGAGGGCCG GCCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTTCGA GAGCGACGCA
    GACACGGCCA CGGCCCACAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
    GTGGCTGCTG AGCACCTCAC CCGGAAGCGC CCAGGGGCTG AGGCCACGGG GAAGGTGAAT
    GTCAAGACGC TGCGTCTGGG CCCGCTCACC AAGGCCGGCT TCTACCTGGC CTTCCAGGAC
    CAGGGTGCCT GCATGGCCCT GCTGTCCCTG CACCTCTTCT ATAAGAAGTG CGCCCAGCAG
    ACTGTGAACC TCACCTACTT CCCGGAGACT GTGCCTCGGG AGCTGGTGGT GCCCGTGGCC
    GGCAGCTGCG TGGCCGATGC CATGCCTGCC CCCGGCCCCA GCCCCAGCCT CTACTGCCGG
    GAAGACGGCC AGTGGGCCGA GCAGCCGGTC ACGGGCTGCA GCTGCAATGC AGGGTTCGAG
    GCCGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCTCAGG GCACCTTCAA GCCCCTGTCC
    GGGGAGGGGT CCTGCCAGCC GTGCCCAGCC AACAGCCACT CTAACGCCAT TGGCTCGTCC
    ATCTGCCAGT GTCGCATCGG GTACTTCCGG GCCAGCACAG ACCCCCGAAG TGCGCCCTGT
    ACCACACCCC CGTCAGCCCC GAGAAGCGTG GTTCCCCGCC TGAATGGCTC CGCCCTGCGC
    TTGGAGTGGA GTGCGCCCCT CGAGTCAGGC GGCCGGGAGG ACCTCACTTA CGCGCTCCGC
    TGCCGGGAGT GCCGGCCCGG TGGGTCCTGC ACGCCCTGCG GGGGAGACCT GACCTTCGAC
    CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGCGATTC GCGGTCTGCG TCCTGACGTC
    ACCTATACGT TTGAGGTCAC CGCCTTGAAT GGGGTGTCCT CCTTGGCCAG TGGGCCGGTC
    CCGTTTGAGG CTGTGAATGT CACCACGGAC CGCGAGGTGC CACCCCCCGT GTCTGACATC
    CGGGTGACTC GGTCATCACC CAGCAGCTTG AGCCTGGCAT GGGCCGTTCC CCGGGCGCCC
    AGCGGGGCAG TGCTGGACTA CGAGGTCAAG TACCACGAGA AGCCCCTCTC TCCTCCGGAA
    CAGGGCGCTG AGGGCCCCAG CAGCGTGCGG TTCCTGAAGA CGTCAGAGAA CCGGGCAGAG
    CTGCGGGGAC TGAAGCGGGG AGCCAGCTAC CTGGTCCAGG TGCGGGCACG CTCCGAGGCC
    GGCTACGGGC CCTTTGGCCA GGAGCACCAC AGCCAGACCC AGCTGGACGA GAATGAGGCC
    TGGAGGGAGC AGCTGGCCCT GATTGCAGGC ACCGCGGCCG TGGGTGTGGT GCTAGTGTTC
    GTGGTCATCG TCATCGCAGT TCTCTGCCTC AGGAAGCAGA GCAACGGGAG AGAAGCTGAG
    TACTCAGACA AACACGCCCA GTATCTCATT GGGCACGGTA CTAAGGTCTA CATTGACCCC
    TTCACTTACG AAGACCCTAA TGAGGCTGTC AGGGAGTTTG CAAAAGAGAT CGATGTCTCC
    TATGTCAAGA TTGAAGAGGT GATTGGTGCA GGCGAGTTTG GTGAGGTGTG CCGGGGGCGC
    CTCAAGGCCC CTGGGAAGAA GGAGAGCTGC GTAGCCATCA AGACCCTGAA GGGCGGCTAC
    ACGGAGCGGC AGCGGCGGGA GTTCCTGAGT GAGGCCTCCA TCATGGGCCA GTTCGAGCAC
    CCCAATATCA TCCGCCTGGA GGGCGTGGTC ACCAACAGCG TGCCCGTCAT GATCCTCACC
    GAGTTCATGG AGAACGGTGC CCTGGACTCC TTCCTGCGGC TGAACGACGG CCAGTTCACC
    GTGATCCAGC TCGTGGGCAT GCTACGGGGC ATCGCCTCGG GCATGCGCTA CCTTGCCGAG
    ATGAGCTACG TCCACCGAGA CCTGGCTGCC CGCAACATCC TGGTCAACAG CAACCTCGTC
    TGCAAGGTCT CAGACTTTGG CCTCTCCCGA TTCCTGGAGG AGAACTCCTC CGACCCCACC
    TACACGAGCT CTCTGGGAGG AAAGATTCCC ATCCGCTGGA CAGCCCCCGA GGCCATTGCC
    TTCCGGAAGT TCACATCTGC CAGTGATGCC TGGAGCTACG GAATTGTCAT GTGGGAGGTC
    ATGTCATTTG GGGAGCGGCC ATACTGGGAT ATGAGCAATC AGGATGTGAT CAACGCCATT
    GAACAGGACT ACCGGTTGCC CCCGCCCCCA GACTGCCCCA CCTCCCTGCA TCAGCTCATG
    CTGGACTGTT GGCAGAAAGA CCGGAATGCG CGGCCCCGCT TCCCCCAGGT GGTCAGCGCC
    CTCGACAAGA TGATCCGGAA CCCTGCCAGC CTCAAAATCG TGGCCCGGGA GAACGGCGGG
    GCCTCGCACC CCCTCCTGGA TCAGCGGCAG CCTCACTACT CAGCTTTTGG CTCCGTGGGG
    GAGTGGCTTC GAGCCATTAA GATGGGAAGA TACGAAGAAA GCTTTGCAGC TGCTGGGTTT
    GGCTCCTTTG AGCTGGTCAG CCAGATCTCC ACTGAGGACC TGCTCCGAAT CGGAGTCACT
    CTGGCGGGAC ACCAGAAGAA AATCCTGGCC AGTGTCCAGC ACATGAAATC CCAGGCCAAG
    CCTGGAGCCC CAGGCGGGTC GGGAGCACCG GCCCCCCAGT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216163
    Clone ID Related Accession (Same CDS sequence) NM_001206268.2
    Accession Version NM_001206268.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product ephrin type-B receptor 4 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02058276.1. On Jun 30, 2017 this sequence version replaced NM_001206268.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822089 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCTCC GGGCTCTGCT CTGCTGGGCT TCGCTTGCAG CCGCCCTAGA AGAGACCCTA 
    CTGAACACGA AATTGGAAAC TGCCGATCTG AAGTGGGTGA CATTCCCTCA GGCGGACGGG
    CAGTGGGAGG AGCTGAGCGG CCTGGACGAG GAGCAGCACA GCGTCCGGAC CTACGAGGTG
    TGTGACATGC AGCGGGCCCC AGGCCTGGCC CACTGGCTGC GCACCGGCTG GGTCCCCCGC
    CGCGGGGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTGGAGTG CCTCTCCCTG
    CCGAGGGCCG GCCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTTCGA GAGCGACGCA
    GACACGGCCA CGGCCCACAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
    GTGGCTGCTG AGCACCTCAC CCGGAAGCGC CCAGGGGCTG AGGCCACGGG GAAGGTGAAT
    GTCAAGACGC TGCGTCTGGG CCCGCTCACC AAGGCCGGCT TCTACCTGGC CTTCCAGGAC
    CAGGGCGCCT GCATGGCCCT GCTGTCCCTG CACCTCTTCT ATAAGAAGTG CGCCCAGCAG
    ACTGTGAACC TCACCTACTT CCCGGAGACT GTGCCTCGGG AGCTGGTGGT GCCCGTGGCC
    GGCAGCTGCG TGGCCGATGC CATGCCTGCC CCCGGCCCCA GCCCCAGCCT CTACTGCCGG
    GAAGACGGCC AGTGGGCCGA GCAGCCGGTC ACGGGCTGCA GCTGCAATGC GGGGTTCGAG
    GCCGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCTCAGG GCACCTTCAA GCCCCTGTCC
    GGGGAGGGGT CCTGCCAGCC GTGCCCAGCC AACAGCCACT CTAACGCCAT TGGCTCGTCC
    ATCTGCCAGT GTCGCATCGG GTACTTCCGG GCCAGCACAG ACCCCCGAAG TGCGCCCTGT
    ACCACACCCC CGTCAGCCCC GAGAAGCGTG GTTCCCCGCC TGAATGGCTC CGCCCTGCGC
    TTGGAGTGGA GTGCGCCCCT CGAGTCAGGC GGCCGGGAGG ACCTCACTTA CGCGCTCCGC
    TGCCGGGAGT GCCGGCCCGG TGGGTCCTGC ACGCCCTGCG GGGGAGACCT GACCTTCGAC
    CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGCGATTC GCGGTCTGCG TCCTGACGTC
    ACCTATACGT TTGAGGTCAC CGCCTTGAAT GGGGTGTCCT CCTTGGCCAG TGGGCCGGTC
    CCGTTTGAGG CTGTGAATGT CACCACGGAC CGCGAGGTGC CACCCCCCGT GTCTGACATC
    CGGGTGACTC GGTCATCACC CAGCAGCTTG AGCCTGGCAT GGGCCGTTCC CCGGGCGCCC
    AGCGGGGCAG TGCTGGACTA CGAGGTCAAG TACCACGAGA AGGGCGCTGA GGGCCCCAGC
    AGCGTGCGGT TCCTGAAGAC GTCAGAGAAC CGGGCAGAGC TGCGGGGACT GAAGCGGGGA
    GCCAGCTACC TGGTCCAGGT GCGGGCACGC TCCGAGGCCG GCTACGGGCC CTTTGGCCAG
    GAGCACCACA GCCAGACCCA GCTGGACGAG AATGAGGCCT GGAGGGAGCA GCTGGCCCTG
    ATTGCAGGCA CCGCGGTCGT GGGTGTGGTG CTAGTGTTCG TGGTCATCGT CATCGCAGTT
    CTCTGCCTCA GGAAGCAGAG CAACGGGAGA GAAGCTGAGT ACTCAGACAA ACACGCCCAG
    TATCTCATTG GGCACGGTAC TAAGGTCTAC ATTGACCCCT TCACTTACGA AGACCCTAAT
    GAGGCTGTCA GGGAGTTTGC AAAAGAGATC GATGTCTCCT ATGTCAAGAT TGAAGAGGTG
    ATTGGTGCAG GCGAGTTTGG TGAGGTGTGC CGGGGGCGCC TCAAGGCCCC TGGGAAGAAG
    GAGAGCTGCG TAGCCATCAA GACCCTGAAG GGCGGCTACA CGGAGCGGCA GCGGCGGGAG
    TTCCTGAGTG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG
    GGCGTGGTCA CCAACAGCGT GCCCGTCATG ATCCTCACCG AGTTCATGGA GAACGGTGCC
    CTGGACTCCT TCCTGCGGCT GAACGACGGC CAGTTCACCG TGATCCAGCT CGTGGGCATG
    CTACGGGGCA TCGCCTCGGG CATGCGCTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC
    CTGGCTGCCC GCAACATCCT GGTCAACAGC AACCTCGTCT GCAAGGTCTC AGACTTTGGC
    CTCTCCCGAT TCCTGGAGGA GAACTCCTCC GACCCCACCT ACACGAGCTC TCTGGGAGGA
    AAGATTCCCA TCCGCTGGAC AGCCCCCGAG GCCATTGCCT TCCGGAAGTT CACATCTGCC
    AGTGATGCCT GGAGCTACGG AATTGTCATG TGGGAGGTCA TGTCATTTGG GGAGCGGCCA
    TACTGGGATA TGAGCAATCA GGATGTGATC AACGCCATTG AACAGGACTA CCGGTTGCCC
    CCGCCCCCAG ACTGCCCCAC CTCCCTGCAT CAGCTCATGC TGGACTGTTG GCAGAAAGAC
    CGGAATGCGC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TCGACAAGAT GATCCGGAAC
    CCTGCCAGCC TCAAAATCGT GGCCCGGGAG AACGGCGGGG CCTCGCACCC CCTCCTGGAT
    CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCCGTGGGGG AGTGGCTTCG AGCCATTAAG
    ATGGGAAGAT ACGAAGAAAG CTTTGCAGCT GCTGGGTTTG GCTCCTTTGA GCTGGTCAGC
    CAGATCTCCA CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA
    ATCCTGGCCA GTGTCCAGCA CATGAAATCC CAGGCCAAGC CTGGAGCCCC AGGCGGGTCG
    GGAGCACCGG CCCCCCAGTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193197.2
    CDS1..2964
    Translation

    Target ORF information:

    RefSeq Version NM_001206268.2
    Organism Bos taurus(cattle)
    Definition Bos taurus EPH receptor B4 (EPHB4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206268.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCTCC GGGCTCTGCT CTGCTGGGCT TCGCTTGCAG CCGCCCTAGA AGAGACCCTA 
    CTGAACACGA AATTGGAAAC TGCCGATCTG AAGTGGGTGA CATTCCCTCA GGCGGACGGG
    CAGTGGGAGG AGCTGAGCGG CCTGGACGAG GAGCAGCACA GCGTCCGGAC CTACGAGGTG
    TGTGACATGC AGCGGGCCCC AGGCCTGGCC CACTGGCTGC GCACCGGCTG GGTCCCCCGC
    CGCGGGGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTGGAGTG CCTCTCCCTG
    CCGAGGGCCG GCCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTTCGA GAGCGACGCA
    GACACGGCCA CGGCCCACAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
    GTGGCTGCTG AGCACCTCAC CCGGAAGCGC CCAGGGGCTG AGGCCACGGG GAAGGTGAAT
    GTCAAGACGC TGCGTCTGGG CCCGCTCACC AAGGCCGGCT TCTACCTGGC CTTCCAGGAC
    CAGGGCGCCT GCATGGCCCT GCTGTCCCTG CACCTCTTCT ATAAGAAGTG CGCCCAGCAG
    ACTGTGAACC TCACCTACTT CCCGGAGACT GTGCCTCGGG AGCTGGTGGT GCCCGTGGCC
    GGCAGCTGCG TGGCCGATGC CATGCCTGCC CCCGGCCCCA GCCCCAGCCT CTACTGCCGG
    GAAGACGGCC AGTGGGCCGA GCAGCCGGTC ACGGGCTGCA GCTGCAATGC GGGGTTCGAG
    GCCGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCTCAGG GCACCTTCAA GCCCCTGTCC
    GGGGAGGGGT CCTGCCAGCC GTGCCCAGCC AACAGCCACT CTAACGCCAT TGGCTCGTCC
    ATCTGCCAGT GTCGCATCGG GTACTTCCGG GCCAGCACAG ACCCCCGAAG TGCGCCCTGT
    ACCACACCCC CGTCAGCCCC GAGAAGCGTG GTTCCCCGCC TGAATGGCTC CGCCCTGCGC
    TTGGAGTGGA GTGCGCCCCT CGAGTCAGGC GGCCGGGAGG ACCTCACTTA CGCGCTCCGC
    TGCCGGGAGT GCCGGCCCGG TGGGTCCTGC ACGCCCTGCG GGGGAGACCT GACCTTCGAC
    CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGCGATTC GCGGTCTGCG TCCTGACGTC
    ACCTATACGT TTGAGGTCAC CGCCTTGAAT GGGGTGTCCT CCTTGGCCAG TGGGCCGGTC
    CCGTTTGAGG CTGTGAATGT CACCACGGAC CGCGAGGTGC CACCCCCCGT GTCTGACATC
    CGGGTGACTC GGTCATCACC CAGCAGCTTG AGCCTGGCAT GGGCCGTTCC CCGGGCGCCC
    AGCGGGGCAG TGCTGGACTA CGAGGTCAAG TACCACGAGA AGGGCGCTGA GGGCCCCAGC
    AGCGTGCGGT TCCTGAAGAC GTCAGAGAAC CGGGCAGAGC TGCGGGGACT GAAGCGGGGA
    GCCAGCTACC TGGTCCAGGT GCGGGCACGC TCCGAGGCCG GCTACGGGCC CTTTGGCCAG
    GAGCACCACA GCCAGACCCA GCTGGACGAG AATGAGGCCT GGAGGGAGCA GCTGGCCCTG
    ATTGCAGGCA CCGCGGTCGT GGGTGTGGTG CTAGTGTTCG TGGTCATCGT CATCGCAGTT
    CTCTGCCTCA GGAAGCAGAG CAACGGGAGA GAAGCTGAGT ACTCAGACAA ACACGCCCAG
    TATCTCATTG GGCACGGTAC TAAGGTCTAC ATTGACCCCT TCACTTACGA AGACCCTAAT
    GAGGCTGTCA GGGAGTTTGC AAAAGAGATC GATGTCTCCT ATGTCAAGAT TGAAGAGGTG
    ATTGGTGCAG GCGAGTTTGG TGAGGTGTGC CGGGGGCGCC TCAAGGCCCC TGGGAAGAAG
    GAGAGCTGCG TAGCCATCAA GACCCTGAAG GGCGGCTACA CGGAGCGGCA GCGGCGGGAG
    TTCCTGAGTG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG
    GGCGTGGTCA CCAACAGCGT GCCCGTCATG ATCCTCACCG AGTTCATGGA GAACGGTGCC
    CTGGACTCCT TCCTGCGGCT GAACGACGGC CAGTTCACCG TGATCCAGCT CGTGGGCATG
    CTACGGGGCA TCGCCTCGGG CATGCGCTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC
    CTGGCTGCCC GCAACATCCT GGTCAACAGC AACCTCGTCT GCAAGGTCTC AGACTTTGGC
    CTCTCCCGAT TCCTGGAGGA GAACTCCTCC GACCCCACCT ACACGAGCTC TCTGGGAGGA
    AAGATTCCCA TCCGCTGGAC AGCCCCCGAG GCCATTGCCT TCCGGAAGTT CACATCTGCC
    AGTGATGCCT GGAGCTACGG AATTGTCATG TGGGAGGTCA TGTCATTTGG GGAGCGGCCA
    TACTGGGATA TGAGCAATCA GGATGTGATC AACGCCATTG AACAGGACTA CCGGTTGCCC
    CCGCCCCCAG ACTGCCCCAC CTCCCTGCAT CAGCTCATGC TGGACTGTTG GCAGAAAGAC
    CGGAATGCGC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TCGACAAGAT GATCCGGAAC
    CCTGCCAGCC TCAAAATCGT GGCCCGGGAG AACGGCGGGG CCTCGCACCC CCTCCTGGAT
    CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCCGTGGGGG AGTGGCTTCG AGCCATTAAG
    ATGGGAAGAT ACGAAGAAAG CTTTGCAGCT GCTGGGTTTG GCTCCTTTGA GCTGGTCAGC
    CAGATCTCCA CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA
    ATCCTGGCCA GTGTCCAGCA CATGAAATCC CAGGCCAAGC CTGGAGCCCC AGGCGGGTCG
    GGAGCACCGG CCCCCCAGTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37585
    Clone ID Related Accession (Same CDS sequence) NM_001206268.1
    Accession Version NM_001206268.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product ephrin type-B receptor 4 precursor
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03048038.1. On Apr 20, 2011 this sequence version replaced XM_869400.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN01093727 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCTCC GGGCTCTGCT CTGCTGGGCT TCGCTTGCAG CCGCCCTAGA AGAGACCCTA 
    CTGAACACGA AATTGGAAAC TGCCGATCTG AAGTGGGTGA CATTCCCTCA GGCGGACGGG
    CAGTGGGAGG AGCTGAGCGG CCTGGACGAG GAGCAGCACA GCGTCCGGAC CTACGAGGTG
    TGTGACATGC AGCGGGCCCC AGGCCTGGCC CACTGGCTGC GCACCGGCTG GGTCCCCCGC
    CGCGGGGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTGGAGTG CCTCTCCCTG
    CCGAGGGCCG GCCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTTCGA GAGCGACGCA
    GACACGGCCA CGGCCCACAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
    GTGGCTGCTG AGCACCTCAC CCGGAAGCGC CCAGGGGCTG AGGCCACGGG GAAGGTGAAT
    GTCAAGACGC TGCGTCTGGG CCCGCTCACC AAGGCCGGCT TCTACCTGGC CTTCCAGGAC
    CAGGGTGCCT GCATGGCCCT GCTGTCCCTG CACCTCTTCT ATAAGAAGTG CGCCCAGCAG
    ACTGTGAACC TCACCTACTT CCCGGAGACT GTGCCTCGGG AGCTGGTGGT GCCCGTGGCC
    GGCAGCTGCG TGGCCGATGC CATGCCTGCC CCCGGCCCCA GCCCCAGCCT CTACTGCCGG
    GAAGACGGCC AGTGGGCCGA GCAGCCGGTC ACGGGCTGCA GCTGCAATGC GGGGTTCGAG
    GCCGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCTCAGG GCACCTTCAA GCCCCTGTCC
    GGGGAGGGGT CCTGCCAGCC GTGCCCAGCC AACAGCCACT CTAACGCCAT TGGCTCGTCC
    ATCTGCCAGT GTCGCATCGG GTACTTCCGG GCCAGCACAG ACCCCCGAAG TGCGCCCTGT
    ACCACACCCC CGTCAGCCCC GAGAAGCGTG GTTCCCCGCC TGAATGGCTC CGCCCTGCGC
    TTGGAGTGGA GTGCGCCCCT CGAGTCAGGC GGCCGGGAGG ACCTCACTTA CGCGCTCCGC
    TGCCGGGAGT GCCGGCCCGG TGGGTCCTGC ACGCCCTGCG GGGGAGACCT GACCTTCGAC
    CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGCGATTC GCGGTCTGCG TCCTGACGTC
    ACCTATACGT TTGAGGTCAC CGCCTTGAAT GGGGTGTCCT CCTTGGCCAG TGGGCCGGTC
    CCGTTTGAGG CTGTGAATGT CACCACGGAC CGCGAGGTGC CACCCCCCGT GTCTGACATC
    CGGGTGACTC GGTCATCACC CAGCAGCTTG AGCCTGGCAT GGGCCGTTCC CCGGGCGCCC
    AGCGGGGCAG TGCTGGACTA CGAGGTCAAG TACCACGAGA AGGGCGCTGA GGGCCCCAGC
    AGCGTGCGGT TCCTGAAGAC GTCAGAGAAC CGGGCAGAGC TGCGGGGACT GAAGCGGGGA
    GCCAGCTACC TGGTCCAGGT GCGGGCACGC TCCGAGGCCG GCTACGGGCC CTTTGGCCAG
    GAGCACCACA GCCAGACCCA GCTGGACGAG AATGAGGCCT GGAGGGAGCA GCTGGCCCTG
    ATTGCAGGCA CCGCGGCCGT GGGTGTGGTG CTAGTGCTCG TGGTCATCGT CATCGCAGTT
    CTCTGCCTCA GGAAGCAGAG CAACGGGAGA GAAGCTGAGT ACTCAGACAA ACACGCCCAG
    TATCTCATTG GGCACGGTAC TAAGGTCTAC ATTGACCCCT TCACTTACGA AGACCCTAAT
    GAGGCTGTCA GGGAGTTTGC AAAAGAGATC GATGTCTCCT ATGTCAAGAT TGAAGAGGTG
    ATTGGTGCAG GCGAGTTTGG TGAGGTGTGC CGGGGGCGCC TCAAGGCCCC TGGGAAGAAG
    GAGAGCTGCG TAGCCATCAA GACCCTGAAG GGCGGCTACA CGGAGCGGCA GCGGCGGGAG
    TTCCTGAGTG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG
    GGCGTGGTCA CCAACAGCGT GCCCGTCATG ATCCTCACCG AGTTCATGGA GAACGGTGCC
    CTGGACTCCT TCCTGCGGCT GAACGACGGC CAGTTCACCG TGATCCAGCT CGTGGGCATG
    CTACGGGGCA TCGCCTCGGG CATGCGCTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC
    CTGGCTGCCC GCAACATCCT GGTCAACAGC AACCTCGTCT GCAAGGTCTC AGACTTTGGC
    CTCTCCCGAT TCCTGGAGGA GAACTCCTCC GACCCCACCT ACACGAGCTC TCTGGGAGGA
    AAGATTCCCA TCCGCTGGAC AGCCCCCGAG GCCATTGCCT TCCGGAAGTT CACATCTGCC
    AGTGATGCCT GGAGCTACGG AATTGTCATG TGGGAGGTCA TGTCATTTGG GGAGCGGCCA
    TACTGGGATA TGAGCAATCA GGATGTGATC AACGCCATTG AACAGGACTA CCGGTTGCCC
    CCGCCCCCAG ACTGCCCCAC CTCCCTGCAT CAGCTCATGC TGGACTGTTG GCAGAAAGAC
    CGGAATGCGC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TCGACAAGAT GATCCGGAAC
    CCTGCCAGCC TCAAAATCGT GGCCCGGGAG AACGGCGGGG CCTCGCACCC CCTCCTGGAT
    CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCCGTGGGGG AGTGGCTTCG AGCCATTAAG
    ATGGGAAGAT ACGAAGAAAG CTTTGCAGCT GCTGGGTTTG GCTCCTTTGA GCTGGTCAGC
    CAGATCTCCA CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA
    ATCCTGGCCA GTGTCCAGCA CATGAAATCC CAGGCCAAGC CTGGAGCCCC AGGCGGGTCG
    GGAGCACCGG CCCCCCAGTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193197.1
    CDS1..2964
    Translation

    Target ORF information:

    RefSeq Version NM_001206268.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus EPH receptor B4 (EPHB4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206268.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCTCC GGGCTCTGCT CTGCTGGGCT TCGCTTGCAG CCGCCCTAGA AGAGACCCTA 
    CTGAACACGA AATTGGAAAC TGCCGATCTG AAGTGGGTGA CATTCCCTCA GGCGGACGGG
    CAGTGGGAGG AGCTGAGCGG CCTGGACGAG GAGCAGCACA GCGTCCGGAC CTACGAGGTG
    TGTGACATGC AGCGGGCCCC AGGCCTGGCC CACTGGCTGC GCACCGGCTG GGTCCCCCGC
    CGCGGGGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTGGAGTG CCTCTCCCTG
    CCGAGGGCCG GCCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTTCGA GAGCGACGCA
    GACACGGCCA CGGCCCACAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
    GTGGCTGCTG AGCACCTCAC CCGGAAGCGC CCAGGGGCTG AGGCCACGGG GAAGGTGAAT
    GTCAAGACGC TGCGTCTGGG CCCGCTCACC AAGGCCGGCT TCTACCTGGC CTTCCAGGAC
    CAGGGTGCCT GCATGGCCCT GCTGTCCCTG CACCTCTTCT ATAAGAAGTG CGCCCAGCAG
    ACTGTGAACC TCACCTACTT CCCGGAGACT GTGCCTCGGG AGCTGGTGGT GCCCGTGGCC
    GGCAGCTGCG TGGCCGATGC CATGCCTGCC CCCGGCCCCA GCCCCAGCCT CTACTGCCGG
    GAAGACGGCC AGTGGGCCGA GCAGCCGGTC ACGGGCTGCA GCTGCAATGC GGGGTTCGAG
    GCCGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCTCAGG GCACCTTCAA GCCCCTGTCC
    GGGGAGGGGT CCTGCCAGCC GTGCCCAGCC AACAGCCACT CTAACGCCAT TGGCTCGTCC
    ATCTGCCAGT GTCGCATCGG GTACTTCCGG GCCAGCACAG ACCCCCGAAG TGCGCCCTGT
    ACCACACCCC CGTCAGCCCC GAGAAGCGTG GTTCCCCGCC TGAATGGCTC CGCCCTGCGC
    TTGGAGTGGA GTGCGCCCCT CGAGTCAGGC GGCCGGGAGG ACCTCACTTA CGCGCTCCGC
    TGCCGGGAGT GCCGGCCCGG TGGGTCCTGC ACGCCCTGCG GGGGAGACCT GACCTTCGAC
    CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGCGATTC GCGGTCTGCG TCCTGACGTC
    ACCTATACGT TTGAGGTCAC CGCCTTGAAT GGGGTGTCCT CCTTGGCCAG TGGGCCGGTC
    CCGTTTGAGG CTGTGAATGT CACCACGGAC CGCGAGGTGC CACCCCCCGT GTCTGACATC
    CGGGTGACTC GGTCATCACC CAGCAGCTTG AGCCTGGCAT GGGCCGTTCC CCGGGCGCCC
    AGCGGGGCAG TGCTGGACTA CGAGGTCAAG TACCACGAGA AGGGCGCTGA GGGCCCCAGC
    AGCGTGCGGT TCCTGAAGAC GTCAGAGAAC CGGGCAGAGC TGCGGGGACT GAAGCGGGGA
    GCCAGCTACC TGGTCCAGGT GCGGGCACGC TCCGAGGCCG GCTACGGGCC CTTTGGCCAG
    GAGCACCACA GCCAGACCCA GCTGGACGAG AATGAGGCCT GGAGGGAGCA GCTGGCCCTG
    ATTGCAGGCA CCGCGGCCGT GGGTGTGGTG CTAGTGCTCG TGGTCATCGT CATCGCAGTT
    CTCTGCCTCA GGAAGCAGAG CAACGGGAGA GAAGCTGAGT ACTCAGACAA ACACGCCCAG
    TATCTCATTG GGCACGGTAC TAAGGTCTAC ATTGACCCCT TCACTTACGA AGACCCTAAT
    GAGGCTGTCA GGGAGTTTGC AAAAGAGATC GATGTCTCCT ATGTCAAGAT TGAAGAGGTG
    ATTGGTGCAG GCGAGTTTGG TGAGGTGTGC CGGGGGCGCC TCAAGGCCCC TGGGAAGAAG
    GAGAGCTGCG TAGCCATCAA GACCCTGAAG GGCGGCTACA CGGAGCGGCA GCGGCGGGAG
    TTCCTGAGTG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG
    GGCGTGGTCA CCAACAGCGT GCCCGTCATG ATCCTCACCG AGTTCATGGA GAACGGTGCC
    CTGGACTCCT TCCTGCGGCT GAACGACGGC CAGTTCACCG TGATCCAGCT CGTGGGCATG
    CTACGGGGCA TCGCCTCGGG CATGCGCTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC
    CTGGCTGCCC GCAACATCCT GGTCAACAGC AACCTCGTCT GCAAGGTCTC AGACTTTGGC
    CTCTCCCGAT TCCTGGAGGA GAACTCCTCC GACCCCACCT ACACGAGCTC TCTGGGAGGA
    AAGATTCCCA TCCGCTGGAC AGCCCCCGAG GCCATTGCCT TCCGGAAGTT CACATCTGCC
    AGTGATGCCT GGAGCTACGG AATTGTCATG TGGGAGGTCA TGTCATTTGG GGAGCGGCCA
    TACTGGGATA TGAGCAATCA GGATGTGATC AACGCCATTG AACAGGACTA CCGGTTGCCC
    CCGCCCCCAG ACTGCCCCAC CTCCCTGCAT CAGCTCATGC TGGACTGTTG GCAGAAAGAC
    CGGAATGCGC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TCGACAAGAT GATCCGGAAC
    CCTGCCAGCC TCAAAATCGT GGCCCGGGAG AACGGCGGGG CCTCGCACCC CCTCCTGGAT
    CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCCGTGGGGG AGTGGCTTCG AGCCATTAAG
    ATGGGAAGAT ACGAAGAAAG CTTTGCAGCT GCTGGGTTTG GCTCCTTTGA GCTGGTCAGC
    CAGATCTCCA CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA
    ATCCTGGCCA GTGTCCAGCA CATGAAATCC CAGGCCAAGC CTGGAGCCCC AGGCGGGTCG
    GGAGCACCGG CCCCCCAGTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49407
    Clone ID Related Accession (Same CDS sequence) XM_010819549.2
    Accession Version XM_010819549.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ephrin type-B receptor 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCTCC GGGCTCTGCT CTGCTGGGCT TCGCTTGCAG CCGCCCTAGA AGAGACCCTA 
    CTGAACACGA AATTGGAAAC TGCCGATCTG AAGTGGGTGA CATTCCCTCA GGCGGACGGG
    CAGTGGGAGG AGCTGAGCGG CCTGGACGAG GAGCAGCACA GCGTCCGGAC CTACGAGGTG
    TGTGACATGC AGCGGGCCCC AGGCCTGGCC CACTGGCTGC GCACCGGCTG GGTCCCCCGC
    CGCGGGGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTGGAGTG CCTCTCCCTG
    CCGAGGGCCG GCCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTTCGA GAGCGACGCA
    GACACGGCCA CGGCCCACAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
    GTGGCTGCTG AGCACCTCAC CCGGAAGCGC CCAGGGGCTG AGGCCACGGG GAAGGTGAAT
    GTCAAGACGC TGCGTCTGGG CCCGCTCACC AAGGCCGGCT TCTACCTGGC CTTCCAGGAC
    CAGGGCGCCT GCATGGCCCT GCTGTCCCTG CACCTCTTCT ATAAGAAGTG CGCCCAGCAG
    ACTGTGAACC TCACCTACTT CCCGGAGACT GTGCCTCGGG AGCTGGTGGT GCCCGTGGCC
    GGCAGCTGCG TGGCCGATGC CATGCCTGCC CCCGGCCCCA GCCCCAGCCT CTACTGCCGG
    GAAGACGGCC AGTGGGCCGA GCAGCCGGTC ACGGGCTGCA GCTGCAATGC GGGGTTCGAG
    GCCGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCTCAGG GCACCTTCAA GCCCCTGTCC
    GGGGAGGGGT CCTGCCAGCC GTGCCCAGCC AACAGCCACT CTAACGCCAT TGGCTCGTCC
    ATCTGCCAGT GTCGCATCGG GTACTTCCGG GCCAGCACAG ACCCCCGAAG TGCGCCCTGT
    ACCACACCCC CGTCAGCCCC GAGAAGCGTG GTTCCCCGCC TGAATGGCTC CGCCCTGCGC
    TTGGAGTGGA GTGCGCCCCT CGAGTCAGGC GGCCGGGAGG ACCTCACTTA CGCGCTCCGC
    TGCCGGGAGT GCCGGCCCGG TGGGTCCTGC ACGCCCTGCG GGGGAGACCT GACCTTCGAC
    CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGCGATTC GCGGTCTGCG TCCTGACGTC
    ACCTATACGT TTGAGGTCAC CGCCTTGAAT GGGGTGTCCT CCTTGGCCAG TGGGCCGGTC
    CCGTTTGAGG CTGTGAATGT CACCACGGAC CGCGAGGTGC CACCCCCCGT GTCTGACATC
    CGGGTGACTC GGTCATCACC CAGCAGCTTG AGCCTGGCAT GGGCCGTTCC CCGGGCGCCC
    AGCGGGGCAG TGCTGGACTA CGAGGTCAAG TACCACGAGA AGCCCCTCTC TCCTCCGGAA
    CAGGGCGCTG AGGGCCCCAG CAGCGTGCGG TTCCTGAAGA CGTCAGAGAA CCGGGCAGAG
    CTGCGGGGAC TGAAGCGGGG AGCCAGCTAC CTGGTCCAGG TGCGGGCACG CTCCGAGGCC
    GGCTACGGGC CCTTTGGCCA GGAGCACCAC AGCCAGACCC AGCTGGACGA GAATGAGGCC
    TGGAGGGAGC AGCTGGCCCT GATTGCAGGC ACCGCGGTCG TGGGTGTGGT GCTAGTGTTC
    GTGGTCATCG TCATCGCAGT TCTCTGCCTC AGGAAGCAGA GCAACGGGAG AGAAGCTGAG
    TACTCAGACA AACACGCCCA GTATCTCATT GGGCACGGTA CTAAGGTCTA CATTGACCCC
    TTCACTTACG AAGACCCTAA TGAGGCTGTC AGGGAGTTTG CAAAAGAGAT CGATGTCTCC
    TATGTCAAGA TTGAAGAGGT GATTGGTGCA GGCGAGTTTG GTGAGGTGTG CCGGGGGCGC
    CTCAAGGCCC CTGGGAAGAA GGAGAGCTGC GTAGCCATCA AGACCCTGAA GGGCGGCTAC
    ACGGAGCGGC AGCGGCGGGA GTTCCTGAGT GAGGCCTCCA TCATGGGCCA GTTCGAGCAC
    CCCAATATCA TCCGCCTGGA GGGCGTGGTC ACCAACAGCG TGCCCGTCAT GATCCTCACC
    GAGTTCATGG AGAACGGTGC CCTGGACTCC TTCCTGCGGC TGAACGACGG CCAGTTCACC
    GTGATCCAGC TCGTGGGCAT GCTACGGGGC ATCGCCTCGG GCATGCGCTA CCTTGCCGAG
    ATGAGCTACG TCCACCGAGA CCTGGCTGCC CGCAACATCC TGGTCAACAG CAACCTCGTC
    TGCAAGGTCT CAGACTTTGG CCTCTCCCGA TTCCTGGAGG AGAACTCCTC CGACCCCACC
    TACACGAGCT CTCTGGGAGG AAAGATTCCC ATCCGCTGGA CAGCCCCCGA GGCCATTGCC
    TTCCGGAAGT TCACATCTGC CAGTGATGCC TGGAGCTACG GAATTGTCAT GTGGGAGGTC
    ATGTCATTTG GGGAGCGGCC ATACTGGGAT ATGAGCAATC AGGATGTGAT CAACGCCATT
    GAACAGGACT ACCGGTTGCC CCCGCCCCCA GACTGCCCCA CCTCCCTGCA TCAGCTCATG
    CTGGACTGTT GGCAGAAAGA CCGGAATGCG CGGCCCCGCT TCCCCCAGGT GGTCAGCGCC
    CTCGACAAGA TGATCCGGAA CCCTGCCAGC CTCAAAATCG TGGCCCGGGA GAACGGCGGG
    GCCTCGCACC CCCTCCTGGA TCAGCGGCAG CCTCACTACT CAGCTTTTGG CTCCGTGGGG
    GAGTGGCTTC GAGCCATTAA GATGGGAAGA TACGAAGAAA GCTTTGCAGC TGCTGGGTTT
    GGCTCCTTTG AGCTGGTCAG CCAGATCTCC ACTGAGGACC TGCTCCGAAT CGGAGTCACT
    CTGGCGGGAC ACCAGAAGAA AATCCTGGCC AGTGTCCAGC ACATGAAATC CCAGGCCAAG
    CCTGGAGCCC CAGGCGGGTC GGGAGCACCG GCCCCCCAGT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817851.1
    CDS381..3365
    Translation

    Target ORF information:

    RefSeq Version XM_010819549.2
    Organism Bos taurus(cattle)
    Definition Bos taurus EPH receptor B4 (EPHB4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819549.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGCTCC GGGCTCTGCT CTGCTGGGCT TCGCTTGCAG CCGCCCTAGA AGAGACCCTA 
    CTGAACACGA AATTGGAAAC TGCCGATCTG AAGTGGGTGA CATTCCCTCA GGCGGACGGG
    CAGTGGGAGG AGCTGAGCGG CCTGGACGAG GAGCAGCACA GCGTCCGGAC CTACGAGGTG
    TGTGACATGC AGCGGGCCCC AGGCCTGGCC CACTGGCTGC GCACCGGCTG GGTCCCCCGC
    CGCGGGGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTGGAGTG CCTCTCCCTG
    CCGAGGGCCG GCCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTTCGA GAGCGACGCA
    GACACGGCCA CGGCCCACAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG
    GTGGCTGCTG AGCACCTCAC CCGGAAGCGC CCAGGGGCTG AGGCCACGGG GAAGGTGAAT
    GTCAAGACGC TGCGTCTGGG CCCGCTCACC AAGGCCGGCT TCTACCTGGC CTTCCAGGAC
    CAGGGCGCCT GCATGGCCCT GCTGTCCCTG CACCTCTTCT ATAAGAAGTG CGCCCAGCAG
    ACTGTGAACC TCACCTACTT CCCGGAGACT GTGCCTCGGG AGCTGGTGGT GCCCGTGGCC
    GGCAGCTGCG TGGCCGATGC CATGCCTGCC CCCGGCCCCA GCCCCAGCCT CTACTGCCGG
    GAAGACGGCC AGTGGGCCGA GCAGCCGGTC ACGGGCTGCA GCTGCAATGC GGGGTTCGAG
    GCCGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCTCAGG GCACCTTCAA GCCCCTGTCC
    GGGGAGGGGT CCTGCCAGCC GTGCCCAGCC AACAGCCACT CTAACGCCAT TGGCTCGTCC
    ATCTGCCAGT GTCGCATCGG GTACTTCCGG GCCAGCACAG ACCCCCGAAG TGCGCCCTGT
    ACCACACCCC CGTCAGCCCC GAGAAGCGTG GTTCCCCGCC TGAATGGCTC CGCCCTGCGC
    TTGGAGTGGA GTGCGCCCCT CGAGTCAGGC GGCCGGGAGG ACCTCACTTA CGCGCTCCGC
    TGCCGGGAGT GCCGGCCCGG TGGGTCCTGC ACGCCCTGCG GGGGAGACCT GACCTTCGAC
    CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGCGATTC GCGGTCTGCG TCCTGACGTC
    ACCTATACGT TTGAGGTCAC CGCCTTGAAT GGGGTGTCCT CCTTGGCCAG TGGGCCGGTC
    CCGTTTGAGG CTGTGAATGT CACCACGGAC CGCGAGGTGC CACCCCCCGT GTCTGACATC
    CGGGTGACTC GGTCATCACC CAGCAGCTTG AGCCTGGCAT GGGCCGTTCC CCGGGCGCCC
    AGCGGGGCAG TGCTGGACTA CGAGGTCAAG TACCACGAGA AGCCCCTCTC TCCTCCGGAA
    CAGGGCGCTG AGGGCCCCAG CAGCGTGCGG TTCCTGAAGA CGTCAGAGAA CCGGGCAGAG
    CTGCGGGGAC TGAAGCGGGG AGCCAGCTAC CTGGTCCAGG TGCGGGCACG CTCCGAGGCC
    GGCTACGGGC CCTTTGGCCA GGAGCACCAC AGCCAGACCC AGCTGGACGA GAATGAGGCC
    TGGAGGGAGC AGCTGGCCCT GATTGCAGGC ACCGCGGTCG TGGGTGTGGT GCTAGTGTTC
    GTGGTCATCG TCATCGCAGT TCTCTGCCTC AGGAAGCAGA GCAACGGGAG AGAAGCTGAG
    TACTCAGACA AACACGCCCA GTATCTCATT GGGCACGGTA CTAAGGTCTA CATTGACCCC
    TTCACTTACG AAGACCCTAA TGAGGCTGTC AGGGAGTTTG CAAAAGAGAT CGATGTCTCC
    TATGTCAAGA TTGAAGAGGT GATTGGTGCA GGCGAGTTTG GTGAGGTGTG CCGGGGGCGC
    CTCAAGGCCC CTGGGAAGAA GGAGAGCTGC GTAGCCATCA AGACCCTGAA GGGCGGCTAC
    ACGGAGCGGC AGCGGCGGGA GTTCCTGAGT GAGGCCTCCA TCATGGGCCA GTTCGAGCAC
    CCCAATATCA TCCGCCTGGA GGGCGTGGTC ACCAACAGCG TGCCCGTCAT GATCCTCACC
    GAGTTCATGG AGAACGGTGC CCTGGACTCC TTCCTGCGGC TGAACGACGG CCAGTTCACC
    GTGATCCAGC TCGTGGGCAT GCTACGGGGC ATCGCCTCGG GCATGCGCTA CCTTGCCGAG
    ATGAGCTACG TCCACCGAGA CCTGGCTGCC CGCAACATCC TGGTCAACAG CAACCTCGTC
    TGCAAGGTCT CAGACTTTGG CCTCTCCCGA TTCCTGGAGG AGAACTCCTC CGACCCCACC
    TACACGAGCT CTCTGGGAGG AAAGATTCCC ATCCGCTGGA CAGCCCCCGA GGCCATTGCC
    TTCCGGAAGT TCACATCTGC CAGTGATGCC TGGAGCTACG GAATTGTCAT GTGGGAGGTC
    ATGTCATTTG GGGAGCGGCC ATACTGGGAT ATGAGCAATC AGGATGTGAT CAACGCCATT
    GAACAGGACT ACCGGTTGCC CCCGCCCCCA GACTGCCCCA CCTCCCTGCA TCAGCTCATG
    CTGGACTGTT GGCAGAAAGA CCGGAATGCG CGGCCCCGCT TCCCCCAGGT GGTCAGCGCC
    CTCGACAAGA TGATCCGGAA CCCTGCCAGC CTCAAAATCG TGGCCCGGGA GAACGGCGGG
    GCCTCGCACC CCCTCCTGGA TCAGCGGCAG CCTCACTACT CAGCTTTTGG CTCCGTGGGG
    GAGTGGCTTC GAGCCATTAA GATGGGAAGA TACGAAGAAA GCTTTGCAGC TGCTGGGTTT
    GGCTCCTTTG AGCTGGTCAG CCAGATCTCC ACTGAGGACC TGCTCCGAAT CGGAGTCACT
    CTGGCGGGAC ACCAGAAGAA AATCCTGGCC AGTGTCCAGC ACATGAAATC CCAGGCCAAG
    CCTGGAGCCC CAGGCGGGTC GGGAGCACCG GCCCCCCAGT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.