APPL2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	APPL2
Entrez Gene ID	515605
Full Name	adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2
General protein information	Preferred Names adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 Names DCC-interacting protein 13-beta adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 amyloid beta (A4) precursor-like protein 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001046206.1	NP_001039671.1	DCC-interacting protein 13-beta
XM_010805164.2	XP_010803466.1	DCC-interacting protein 13-beta isoform X1
XM_010805165.2	XP_010803467.1	DCC-interacting protein 13-beta isoform X2
XM_010805165.1	XP_010803467.1	DCC-interacting protein 13-beta isoform X2
XM_010805164.3	XP_010803466.1	DCC-interacting protein 13-beta isoform X1
XM_024991717.1	XP_024847485.1	DCC-interacting protein 13-beta isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	APPL2	NP_060641.2
Danio rerio (zebrafish)	appl2	NP_001121547.1
Rattus norvegicus (Norway rat)	Appl2	NP_001102211.1
Gallus gallus (chicken)	LOC100857286	XP_003640457.1
	LOC701612	XP_002798807.1
Bos taurus (cattle)	APPL2	NP_001039671.1
Xenopus tropicalis (tropical clawed frog)	appl2	NP_001106389.1
Mus musculus (house mouse)	Appl2	NP_660255.1
Gallus gallus (chicken)	APPL2	XP_416311.2
Pan troglodytes (chimpanzee)	APPL2	XP_001160746.1
Canis lupus familiaris (dog)	APPL2	XP_538418.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following APPL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APPL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40781	NM_001046206.1 Latest version!	Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa49347	XM_010805164.3 Latest version!	Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa49348	XM_010805165.2 Latest version!	Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa234361	XM_024991717.1 Latest version!	Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OBa49348	XM_010805165.1	Bos taurus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa49347	XM_010805164.2	Bos taurus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APPL2 ( NM_001046206.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001039671.1 Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), mRNA.

Price & Availability↑

CloneID	OBa40781
Clone ID Related Accession (Same CDS sequence)	NM_001046206.1
Accession Version	NM_001046206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	DCC-interacting protein 13-beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC114672.1. On or before Sep 7, 2006 this sequence version replaced XM_879709.1, XM_593650.2, XM_879765.1. ##Evidence-Data-START## Transcript exon combination :: BC114672.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GTGTGTTTGA GGAAGATGCC GGCACTCTCA CAGATTATAC CAACCAGCTG 
CTTCAAGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAAA TGTGCCTGGC TACTCAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAATTTTG CTCTTGGCAA AGGCGATGAA 
GAGGTCATTT CTACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TGTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CAATTATACA ATTTCGAGAA 
AAGGATCTTA CAGAAGTAAG CACTTTAAAG GATCTTTTCG GACTTGCCAG CAGCGAGCAT 
GACCTCTCAA TGGCAAAATA TAGCAGGCTT CCTAAAAAAA AGGAGAATGA AAAGGTGAAG 
GCTGAAATCG TGAAGGAGGT GGCCGCAGCC CGGCGAAAAC AGCACCTTTC CTCCCTTCAG 
TACTACTGTG CCCTCAATGC GCTGCAGTAC AGAAAGCGCG TGGCCATGAT GGAGCCCATG 
CTGGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCACAG 
AGTATGGACG GCTTCTTATC CTCCGTCGCA GACATGGTTC AAAGCATCCA GGTGGAACTA 
GAAGTGGAAG CAGAGAAGAT GAGGGCATCC CAGCAAGAAT TACTTTCTCT CGATGAAGCT 
GTTTACACCC CAGACTTCGA TGTGGCTGCA CCCCAGGTCA ACAGGAACCT CATCCAGAAG 
GCCGGTTACC TTAATCTTAG AAATAAAACA GGGCTGGTCT CAACCACCTG GGAGAGACTC 
TACTTCTTCA CCCAAGGCGG GAATCTCATG TGCCAGCCTA GGGGCGCCGT GGCTGGGGGC 
CTGATCCAGG ACCTGGACAA CTGCTCGGTG ATGGCGGTGG ACTGTGAGGA CCGACGATAC 
TGCTTCCAGA TCACCACTCC CAATGGAAAA GCGGGAATAA TCCTCCAGGC AGAAAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACT 
GACAACCCAG AGGCAGTCGC CATCAAGTTG AATCAGACGG CTCTCCAAGC AGTGACTCCA 
ATTACAAGCT TTGCAAAAAA ACAAGAAAGT CCATGCCCCA GCCAGAACGT GAAAAGTTCA 
GAGATGGAAA ATGACAAGAT AGTTCCGAAA GCAACAGTCA CTATACCCGA AGCTGAACAA 
CTGATTGCAC CTGGAACACC TATTCAGTTT GACATTGTAC TTCCTGCTAC AGAATTCCTT 
GATCAGAACA GAGGCGGCAG GCGTACCAAC CCCTTTGGTG AAACTGAGGA TGCGACATTT 
CCAGAAGCAG AAGATTCTCT TTTGCAGCAG ATGTTTATAG TTCGGTTTTT GGGATCAATG 
GCAGTCAAAA CAGACAACAC TACGGAAGTG ATTTACGAAG CAATGAGACA AGTATTGGCT 
GCTCGGGCTA TTCATAACAT CTTCCGGATG ACAGAATCCC ATCTGATGGT CACCAGTCAG 
ACTCTGAGGT TGATAGATCC CCAGACTCAA GTAACAAGGG CCAATTTTGA ACTCACCAGT 
GTCACCCAGT TTGCTGCTCA TCAAGAAAAC AAGAGACTGG TTGGCTTTGT GGTCCGCTTG 
CCTGAATCCA CAGGCGGAGA GTCTCTGAGC ACATATGTTT TTGAAAGCAA CTCAGAAGGC 
GAAAAGATAT GTTATGCTAT TAATTTGGGA AAAGAAATCA TTGAGGTTCA GAAGGATCCA 
GAAGCACTGG CTCAATTAAT GCTGTCCGTA CCACTAACCA ATGATGGAAA ATATGTACTA 
TTAAACGATC AACCAGATGA CAATGATGGA AGTCCAAGTG AAAATAGAGG CGCAGAATCT 
GAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039671.1
CDS	210..2198
Translation	MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNVLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASSEHDLSMAKYSRLPKKKENEKVKAEIVKEVAAARRKQHLSSLQYYCALNALQYRKRVAMMEPMLGFAHGQINFFKKGAEMFSQSMDGFLSSVADMVQSIQVELEVEAEKMRASQQELLSLDEAVYTPDFDVAAPQVNRNLIQKAGYLNLRNKTGLVSTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKAGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFAKKQESPCPSQNVKSSEMENDKIVPKATVTIPEAEQLIAPGTPIQFDIVLPATEFLDQNRGGRRTNPFGETEDATFPEAEDSLLQQMFIVRFLGSMAVKTDNTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQTLRLIDPQTQVTRANFELTSVTQFAAHQENKRLVGFVVRLPESTGGESLSTYVFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSVPLTNDGKYVLLNDQPDDNDGSPSENRGAESEA

Target ORF information:

RefSeq Version	NM_001046206.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001046206.1

ORF Insert Sequence:

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GTGTGTTTGA GGAAGATGCC GGCACTCTCA CAGATTATAC CAACCAGCTG 
CTTCAAGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAAA TGTGCCTGGC TACTCAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAATTTTG CTCTTGGCAA AGGCGATGAA 
GAGGTCATTT CTACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TGTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CAATTATACA ATTTCGAGAA 
AAGGATCTTA CAGAAGTAAG CACTTTAAAG GATCTTTTCG GACTTGCCAG CAGCGAGCAT 
GACCTCTCAA TGGCAAAATA TAGCAGGCTT CCTAAAAAAA AGGAGAATGA AAAGGTGAAG 
GCTGAAATCG TGAAGGAGGT GGCCGCAGCC CGGCGAAAAC AGCACCTTTC CTCCCTTCAG 
TACTACTGTG CCCTCAATGC GCTGCAGTAC AGAAAGCGCG TGGCCATGAT GGAGCCCATG 
CTGGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCACAG 
AGTATGGACG GCTTCTTATC CTCCGTCGCA GACATGGTTC AAAGCATCCA GGTGGAACTA 
GAAGTGGAAG CAGAGAAGAT GAGGGCATCC CAGCAAGAAT TACTTTCTCT CGATGAAGCT 
GTTTACACCC CAGACTTCGA TGTGGCTGCA CCCCAGGTCA ACAGGAACCT CATCCAGAAG 
GCCGGTTACC TTAATCTTAG AAATAAAACA GGGCTGGTCT CAACCACCTG GGAGAGACTC 
TACTTCTTCA CCCAAGGCGG GAATCTCATG TGCCAGCCTA GGGGCGCCGT GGCTGGGGGC 
CTGATCCAGG ACCTGGACAA CTGCTCGGTG ATGGCGGTGG ACTGTGAGGA CCGACGATAC 
TGCTTCCAGA TCACCACTCC CAATGGAAAA GCGGGAATAA TCCTCCAGGC AGAAAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACT 
GACAACCCAG AGGCAGTCGC CATCAAGTTG AATCAGACGG CTCTCCAAGC AGTGACTCCA 
ATTACAAGCT TTGCAAAAAA ACAAGAAAGT CCATGCCCCA GCCAGAACGT GAAAAGTTCA 
GAGATGGAAA ATGACAAGAT AGTTCCGAAA GCAACAGTCA CTATACCCGA AGCTGAACAA 
CTGATTGCAC CTGGAACACC TATTCAGTTT GACATTGTAC TTCCTGCTAC AGAATTCCTT 
GATCAGAACA GAGGCGGCAG GCGTACCAAC CCCTTTGGTG AAACTGAGGA TGCGACATTT 
CCAGAAGCAG AAGATTCTCT TTTGCAGCAG ATGTTTATAG TTCGGTTTTT GGGATCAATG 
GCAGTCAAAA CAGACAACAC TACGGAAGTG ATTTACGAAG CAATGAGACA AGTATTGGCT 
GCTCGGGCTA TTCATAACAT CTTCCGGATG ACAGAATCCC ATCTGATGGT CACCAGTCAG 
ACTCTGAGGT TGATAGATCC CCAGACTCAA GTAACAAGGG CCAATTTTGA ACTCACCAGT 
GTCACCCAGT TTGCTGCTCA TCAAGAAAAC AAGAGACTGG TTGGCTTTGT GGTCCGCTTG 
CCTGAATCCA CAGGCGGAGA GTCTCTGAGC ACATATGTTT TTGAAAGCAA CTCAGAAGGC 
GAAAAGATAT GTTATGCTAT TAATTTGGGA AAAGAAATCA TTGAGGTTCA GAAGGATCCA 
GAAGCACTGG CTCAATTAAT GCTGTCCGTA CCACTAACCA ATGATGGAAA ATATGTACTA 
TTAAACGATC AACCAGATGA CAATGATGGA AGTCCAAGTG AAAATAGAGG CGCAGAATCT 
GAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_010805164.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803466.1 Bos taurus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa49347
Clone ID Related Accession (Same CDS sequence)	XM_010805164.2 , XM_010805164.3
Accession Version	XM_010805164.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DCC-interacting protein 13-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805164.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGAATGAA 
ATGTGCCTGG CTACTCAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAATTTT 
GCTCTTGGCA AAGGCGATGA AGAGGTCATT TCTACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATGTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCAATTATAC AATTTCGAGA AAAGGATCTT ACAGAAGTAA GCACTTTAAA GGATCTTTTC 
GGACTTGCCA GCAGCGAGCA TGACCTCTCA ATGGCAAAAT ATAGCAGGCT TCCTAAAAAA 
AAGGAGAATG AAAAGGTGAA GGCTGAAATC GTGAAGGAGG TGGCCGCAGC CCGGCGAAAA 
CAGCACCTTT CCTCCCTTCA GTACTACTGT GCCCTCAATG CGCTGCAGTA CAGAAAGCGC 
GTGGCCATGA TGGAGCCCAT GCTGGGCTTT GCCCATGGAC AGATTAACTT TTTTAAGAAG 
GGAGCAGAGA TGTTTTCACA GAGTATGGAC GGCTTCTTAT CCTCCGTCGC AGACATGGTT 
CAAAGCATCC AGGTGGAACT AGAAGTGGAA GCAGAGAAGA TGAGGGCATC CCAGCAAGAA 
TTACTTTCTC TCGATGAAGC TGTTTACACC CCAGACTTCG ATGTGGCTGC ACCCCAGGTC 
AACAGGAACC TCATCCAGAA GGCCGGTTAC CTTAATCTTA GAAATAAAAC AGGGCTGGTC 
TCAACCACCT GGGAGAGACT CTACTTCTTC ACCCAAGGCG GGAATCTCAT GTGCCAGCCT 
AGGGGCGCCG TGGCTGGGGG CCTGATCCAG GACCTGGACA ACTGCTCGGT GATGGCGGTG 
GACTGTGAGG ACCGACGATA CTGCTTCCAG ATCACCACTC CCAATGGAAA AGCGGGAATA 
ATCCTCCAGG CAGAAAGCAG AAAGGAAAAT GAAGAGTGGA TATGTGCAAT AAACAACATC 
TCCAGACAGA TCTACCTGAC TGACAACCCA GAGGCAGTCG CCATCAAGTT GAATCAGACG 
GCTCTCCAAG CAGTGACTCC AATTACAAGC TTTGCAAAAA AACAAGAAAG TCCATGCCCC 
AGCCAGAACG TGAAAAGTTC AGAGATGGAA AATGACAAGA TAGTTCCGAA AGCAACAGTC 
ACTATACCCG AAGCTGAACA ACTGATTGCA CCTGGAACAC CTATTCAGTT TGACATTGTA 
CTTCCTGCTA CAGAATTCCT TGATCAGAAC AGAGGCGGCA GGCGTACCAA CCCCTTTGGT 
GAAACTGAGG ATGCGACATT TCCAGAAGCA GAAGATTCTC TTTTGCAGCA GATGTTTATA 
GTTCGGTTTT TGGGATCAAT GGCAGTCAAA ACAGACAACA CTACGGAAGT GATTTACGAA 
GCAATGAGAC AAGTATTGGC TGCTCGGGCT ATTCATAACA TCTTCCGGAT GACAGAATCC 
CATCTGATGG TCACCAGTCA GACTCTGAGG TTGATAGATC CCCAGACTCA AGTAACAAGG 
GCCAATTTTG AACTCACCAG TGTCACCCAG TTTGCTGCTC ATCAAGAAAA CAAGAGACTG 
GTTGGCTTTG TGGTCCGCTT GCCTGAATCC ACAGGCGGAG AGTCTCTGAG CACATATGTT 
TTTGAAAGCA ACTCAGAAGG CGAAAAGATA TGTTATGCTA TTAATTTGGG AAAAGAAATC 
ATTGAGGTTC AGAAGGATCC AGAAGCACTG GCTCAATTAA TGCTGTCCGT ACCACTAACC 
AATGATGGAA AATATGTACT ATTAAACGAT CAACCAGATG ACAATGATGG AAGTCCAAGT 
GAAAATAGAG GCGCAGAATC TGAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803466.1
CDS	159..2048
Translation	MPAVDKLLLEEALQDSPQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNVLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASSEHDLSMAKYSRLPKKKENEKVKAEIVKEVAAARRKQHLSSLQYYCALNALQYRKRVAMMEPMLGFAHGQINFFKKGAEMFSQSMDGFLSSVADMVQSIQVELEVEAEKMRASQQELLSLDEAVYTPDFDVAAPQVNRNLIQKAGYLNLRNKTGLVSTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKAGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFAKKQESPCPSQNVKSSEMENDKIVPKATVTIPEAEQLIAPGTPIQFDIVLPATEFLDQNRGGRRTNPFGETEDATFPEAEDSLLQQMFIVRFLGSMAVKTDNTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQTLRLIDPQTQVTRANFELTSVTQFAAHQENKRLVGFVVRLPESTGGESLSTYVFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSVPLTNDGKYVLLNDQPDDNDGSPSENRGAESEA

Target ORF information:

RefSeq Version	XM_010805164.2
Organism	Bos taurus(cattle)
Definition	Bos taurus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805164.2

ORF Insert Sequence:

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGAATGAA 
ATGTGCCTGG CTACTCAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAATTTT 
GCTCTTGGCA AAGGCGATGA AGAGGTCATT TCTACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATGTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCAATTATAC AATTTCGAGA AAAGGATCTT ACAGAAGTAA GCACTTTAAA GGATCTTTTC 
GGACTTGCCA GCAGCGAGCA TGACCTCTCA ATGGCAAAAT ATAGCAGGCT TCCTAAAAAA 
AAGGAGAATG AAAAGGTGAA GGCTGAAATC GTGAAGGAGG TGGCCGCAGC CCGGCGAAAA 
CAGCACCTTT CCTCCCTTCA GTACTACTGT GCCCTCAATG CGCTGCAGTA CAGAAAGCGC 
GTGGCCATGA TGGAGCCCAT GCTGGGCTTT GCCCATGGAC AGATTAACTT TTTTAAGAAG 
GGAGCAGAGA TGTTTTCACA GAGTATGGAC GGCTTCTTAT CCTCCGTCGC AGACATGGTT 
CAAAGCATCC AGGTGGAACT AGAAGTGGAA GCAGAGAAGA TGAGGGCATC CCAGCAAGAA 
TTACTTTCTC TCGATGAAGC TGTTTACACC CCAGACTTCG ATGTGGCTGC ACCCCAGGTC 
AACAGGAACC TCATCCAGAA GGCCGGTTAC CTTAATCTTA GAAATAAAAC AGGGCTGGTC 
TCAACCACCT GGGAGAGACT CTACTTCTTC ACCCAAGGCG GGAATCTCAT GTGCCAGCCT 
AGGGGCGCCG TGGCTGGGGG CCTGATCCAG GACCTGGACA ACTGCTCGGT GATGGCGGTG 
GACTGTGAGG ACCGACGATA CTGCTTCCAG ATCACCACTC CCAATGGAAA AGCGGGAATA 
ATCCTCCAGG CAGAAAGCAG AAAGGAAAAT GAAGAGTGGA TATGTGCAAT AAACAACATC 
TCCAGACAGA TCTACCTGAC TGACAACCCA GAGGCAGTCG CCATCAAGTT GAATCAGACG 
GCTCTCCAAG CAGTGACTCC AATTACAAGC TTTGCAAAAA AACAAGAAAG TCCATGCCCC 
AGCCAGAACG TGAAAAGTTC AGAGATGGAA AATGACAAGA TAGTTCCGAA AGCAACAGTC 
ACTATACCCG AAGCTGAACA ACTGATTGCA CCTGGAACAC CTATTCAGTT TGACATTGTA 
CTTCCTGCTA CAGAATTCCT TGATCAGAAC AGAGGCGGCA GGCGTACCAA CCCCTTTGGT 
GAAACTGAGG ATGCGACATT TCCAGAAGCA GAAGATTCTC TTTTGCAGCA GATGTTTATA 
GTTCGGTTTT TGGGATCAAT GGCAGTCAAA ACAGACAACA CTACGGAAGT GATTTACGAA 
GCAATGAGAC AAGTATTGGC TGCTCGGGCT ATTCATAACA TCTTCCGGAT GACAGAATCC 
CATCTGATGG TCACCAGTCA GACTCTGAGG TTGATAGATC CCCAGACTCA AGTAACAAGG 
GCCAATTTTG AACTCACCAG TGTCACCCAG TTTGCTGCTC ATCAAGAAAA CAAGAGACTG 
GTTGGCTTTG TGGTCCGCTT GCCTGAATCC ACAGGCGGAG AGTCTCTGAG CACATATGTT 
TTTGAAAGCA ACTCAGAAGG CGAAAAGATA TGTTATGCTA TTAATTTGGG AAAAGAAATC 
ATTGAGGTTC AGAAGGATCC AGAAGCACTG GCTCAATTAA TGCTGTCCGT ACCACTAACC 
AATGATGGAA AATATGTACT ATTAAACGAT CAACCAGATG ACAATGATGG AAGTCCAAGT 
GAAAATAGAG GCGCAGAATC TGAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_010805164.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803466.1 Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa49347
Clone ID Related Accession (Same CDS sequence)	XM_010805164.2 , XM_010805164.3
Accession Version	XM_010805164.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DCC-interacting protein 13-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805164.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGAATGAA 
ATGTGCCTGG CTACTCAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAATTTT 
GCTCTTGGCA AAGGCGATGA AGAGGTCATT TCTACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATGTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCAATTATAC AATTTCGAGA AAAGGATCTT ACAGAAGTAA GCACTTTAAA GGATCTTTTC 
GGACTTGCCA GCAGCGAGCA TGACCTCTCA ATGGCAAAAT ATAGCAGGCT TCCTAAAAAA 
AAGGAGAATG AAAAGGTGAA GGCTGAAATC GTGAAGGAGG TGGCCGCAGC CCGGCGAAAA 
CAGCACCTTT CCTCCCTTCA GTACTACTGT GCCCTCAATG CGCTGCAGTA CAGAAAGCGC 
GTGGCCATGA TGGAGCCCAT GCTGGGCTTT GCCCATGGAC AGATTAACTT TTTTAAGAAG 
GGAGCAGAGA TGTTTTCACA GAGTATGGAC GGCTTCTTAT CCTCCGTCGC AGACATGGTT 
CAAAGCATCC AGGTGGAACT AGAAGTGGAA GCAGAGAAGA TGAGGGCATC CCAGCAAGAA 
TTACTTTCTC TCGATGAAGC TGTTTACACC CCAGACTTCG ATGTGGCTGC ACCCCAGGTC 
AACAGGAACC TCATCCAGAA GGCCGGTTAC CTTAATCTTA GAAATAAAAC AGGGCTGGTC 
TCAACCACCT GGGAGAGACT CTACTTCTTC ACCCAAGGCG GGAATCTCAT GTGCCAGCCT 
AGGGGCGCCG TGGCTGGGGG CCTGATCCAG GACCTGGACA ACTGCTCGGT GATGGCGGTG 
GACTGTGAGG ACCGACGATA CTGCTTCCAG ATCACCACTC CCAATGGAAA AGCGGGAATA 
ATCCTCCAGG CAGAAAGCAG AAAGGAAAAT GAAGAGTGGA TATGTGCAAT AAACAACATC 
TCCAGACAGA TCTACCTGAC TGACAACCCA GAGGCAGTCG CCATCAAGTT GAATCAGACG 
GCTCTCCAAG CAGTGACTCC AATTACAAGC TTTGCAAAAA AACAAGAAAG TCCATGCCCC 
AGCCAGAACG TGAAAAGTTC AGAGATGGAA AATGACAAGA TAGTTCCGAA AGCAACAGTC 
ACTATACCCG AAGCTGAACA ACTGATTGCA CCTGGAACAC CTATTCAGTT TGACATTGTA 
CTTCCTGCTA CAGAATTCCT TGATCAGAAC AGAGGCGGCA GGCGTACCAA CCCCTTTGGT 
GAAACTGAGG ATGCGACATT TCCAGAAGCA GAAGATTCTC TTTTGCAGCA GATGTTTATA 
GTTCGGTTTT TGGGATCAAT GGCAGTCAAA ACAGACAACA CTACGGAAGT GATTTACGAA 
GCAATGAGAC AAGTATTGGC TGCTCGGGCT ATTCATAACA TCTTCCGGAT GACAGAATCC 
CATCTGATGG TCACCAGTCA GACTCTGAGG TTGATAGATC CCCAGACTCA AGTAACAAGG 
GCCAATTTTG AACTCACCAG TGTCACCCAG TTTGCTGCTC ATCAAGAAAA CAAGAGACTG 
GTTGGCTTTG TGGTCCGCTT GCCTGAATCC ACAGGCGGAG AGTCTCTGAG CACATATGTT 
TTTGAAAGCA ACTCAGAAGG CGAAAAGATA TGTTATGCTA TTAATTTGGG AAAAGAAATC 
ATTGAGGTTC AGAAGGATCC AGAAGCACTG GCTCAATTAA TGCTGTCCGT ACCACTAACC 
AATGATGGAA AATATGTACT ATTAAACGAT CAACCAGATG ACAATGATGG AAGTCCAAGT 
GAAAATAGAG GCGCAGAATC TGAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803466.1
CDS	187..2076
Translation	MPAVDKLLLEEALQDSPQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNVLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASSEHDLSMAKYSRLPKKKENEKVKAEIVKEVAAARRKQHLSSLQYYCALNALQYRKRVAMMEPMLGFAHGQINFFKKGAEMFSQSMDGFLSSVADMVQSIQVELEVEAEKMRASQQELLSLDEAVYTPDFDVAAPQVNRNLIQKAGYLNLRNKTGLVSTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKAGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFAKKQESPCPSQNVKSSEMENDKIVPKATVTIPEAEQLIAPGTPIQFDIVLPATEFLDQNRGGRRTNPFGETEDATFPEAEDSLLQQMFIVRFLGSMAVKTDNTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQTLRLIDPQTQVTRANFELTSVTQFAAHQENKRLVGFVVRLPESTGGESLSTYVFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSVPLTNDGKYVLLNDQPDDNDGSPSENRGAESEA

Target ORF information:

RefSeq Version	XM_010805164.3
Organism	Bos taurus(cattle)
Definition	Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805164.3

ORF Insert Sequence:

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGAATGAA 
ATGTGCCTGG CTACTCAACA GCTTTCTAAG CAACTGCTGG CATATGAAAA ACAGAATTTT 
GCTCTTGGCA AAGGCGATGA AGAGGTCATT TCTACACTCC ACTATTTTTC CAAAGTGGTG 
GATGAGCTTA ATGTTCTCCA TACAGAGCTG GCTAAACAGT TGGCAGACAC AATGGTTCTA 
CCAATTATAC AATTTCGAGA AAAGGATCTT ACAGAAGTAA GCACTTTAAA GGATCTTTTC 
GGACTTGCCA GCAGCGAGCA TGACCTCTCA ATGGCAAAAT ATAGCAGGCT TCCTAAAAAA 
AAGGAGAATG AAAAGGTGAA GGCTGAAATC GTGAAGGAGG TGGCCGCAGC CCGGCGAAAA 
CAGCACCTTT CCTCCCTTCA GTACTACTGT GCCCTCAATG CGCTGCAGTA CAGAAAGCGC 
GTGGCCATGA TGGAGCCCAT GCTGGGCTTT GCCCATGGAC AGATTAACTT TTTTAAGAAG 
GGAGCAGAGA TGTTTTCACA GAGTATGGAC GGCTTCTTAT CCTCCGTCGC AGACATGGTT 
CAAAGCATCC AGGTGGAACT AGAAGTGGAA GCAGAGAAGA TGAGGGCATC CCAGCAAGAA 
TTACTTTCTC TCGATGAAGC TGTTTACACC CCAGACTTCG ATGTGGCTGC ACCCCAGGTC 
AACAGGAACC TCATCCAGAA GGCCGGTTAC CTTAATCTTA GAAATAAAAC AGGGCTGGTC 
TCAACCACCT GGGAGAGACT CTACTTCTTC ACCCAAGGCG GGAATCTCAT GTGCCAGCCT 
AGGGGCGCCG TGGCTGGGGG CCTGATCCAG GACCTGGACA ACTGCTCGGT GATGGCGGTG 
GACTGTGAGG ACCGACGATA CTGCTTCCAG ATCACCACTC CCAATGGAAA AGCGGGAATA 
ATCCTCCAGG CAGAAAGCAG AAAGGAAAAT GAAGAGTGGA TATGTGCAAT AAACAACATC 
TCCAGACAGA TCTACCTGAC TGACAACCCA GAGGCAGTCG CCATCAAGTT GAATCAGACG 
GCTCTCCAAG CAGTGACTCC AATTACAAGC TTTGCAAAAA AACAAGAAAG TCCATGCCCC 
AGCCAGAACG TGAAAAGTTC AGAGATGGAA AATGACAAGA TAGTTCCGAA AGCAACAGTC 
ACTATACCCG AAGCTGAACA ACTGATTGCA CCTGGAACAC CTATTCAGTT TGACATTGTA 
CTTCCTGCTA CAGAATTCCT TGATCAGAAC AGAGGCGGCA GGCGTACCAA CCCCTTTGGT 
GAAACTGAGG ATGCGACATT TCCAGAAGCA GAAGATTCTC TTTTGCAGCA GATGTTTATA 
GTTCGGTTTT TGGGATCAAT GGCAGTCAAA ACAGACAACA CTACGGAAGT GATTTACGAA 
GCAATGAGAC AAGTATTGGC TGCTCGGGCT ATTCATAACA TCTTCCGGAT GACAGAATCC 
CATCTGATGG TCACCAGTCA GACTCTGAGG TTGATAGATC CCCAGACTCA AGTAACAAGG 
GCCAATTTTG AACTCACCAG TGTCACCCAG TTTGCTGCTC ATCAAGAAAA CAAGAGACTG 
GTTGGCTTTG TGGTCCGCTT GCCTGAATCC ACAGGCGGAG AGTCTCTGAG CACATATGTT 
TTTGAAAGCA ACTCAGAAGG CGAAAAGATA TGTTATGCTA TTAATTTGGG AAAAGAAATC 
ATTGAGGTTC AGAAGGATCC AGAAGCACTG GCTCAATTAA TGCTGTCCGT ACCACTAACC 
AATGATGGAA AATATGTACT ATTAAACGAT CAACCAGATG ACAATGATGG AAGTCCAAGT 
GAAAATAGAG GCGCAGAATC TGAAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_010805165.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803467.1 Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa49348
Clone ID Related Accession (Same CDS sequence)	XM_010805165.2 , XM_010805165.1
Accession Version	XM_010805165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DCC-interacting protein 13-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GTGTGTTTGA GGAAGATGCC GGCACTCTCA CAGATTATAC CAACCAGCTG 
CTTCAAGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAAA TGTGCCTGGC TACTCAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAATTTTG CTCTTGGCAA AGGCGATGAA 
GAGGTCATTT CTACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TGTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CAATTATACA ATTTCGAGAA 
AAGGATCTTA CAGAAGTAAG CACTTTAAAG GATCTTTTCG GACTTGCCAG CAGCGAGCAT 
GACCTCTCAA TGGCAAAATA TAGCAGGCTT CCTAAAAAAA AGGAGAATGA AAAGGTGAAG 
GCTGAAATCG TGAAGGAGGT GGCCGCAGCC CGGCGAAAAC AGCACCTTTC CTCCCTTCAG 
TACTACTGTG CCCTCAATGC GCTGCAGTAC AGAAAGCGCG TGGCCATGAT GGAGCCCATG 
CTGGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCACAG 
AGTATGGACG GCTTCTTATC CTCCGTCGCA GACATGGTTC AAAGCATCCA GGTGGAACTA 
GAAGTGGAAG CAGAGAAGAT GAGGGCATCC CAGCAAGAAT TACTTTCTCT CGATGAAGCT 
GTTTACACCC CAGACTTCGA TGTGGCTGCA CCCCAGGTCA ACAGGAACCT CATCCAGAAG 
GCCGGTTACC TTAATCTTAG AAATAAAACA GGGCTGGTCT CAACCACCTG GGAGAGACTC 
TACTTCTTCA CCCAAGGCGG GAATCTCATG TGCCAGCCTA GGGGCGCCGT GGCTGGGGGC 
CTGATCCAGG ACCTGGACAA CTGCTCGGTG ATGGCGGTGG ACTGTGAGGA CCGACGATAC 
TGCTTCCAGA TCACCACTCC CAATGGAAAA GCGGGAATAA TCCTCCAGGC AGAAAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACT 
GACAACCCAG AGGCAGTCGC CATCAAGTTG AATCAGACGG CTCTCCAAGC AGTGACTCCA 
ATTACAAGCT TTGCAAAAAA ACAAGAAAGT CCATGCCCCA GCCAGAACGT GAAAAGTTCA 
GAGATGGAAA ATGACAAGAT AGTTCCGAAA GCAACAGTCA CTATACCCGA AGCTGAACAA 
CTGATTGCAC CTGGAACACC TATTCAGTTT GACATTGTAC TTCCTGCTAC AGAATTCCTT 
GATCAGAACA GAGGCGGCAG GCGTACCAAC CCCTTTGGTG AAACTGAGGA TGCGACATTT 
CCAGAAGCAG AAGATTCTCT TTTGCAGCAG ATGTTTATAG TTCGGTTTTT GGGATCAATG 
GCAGTCAAAA CAGACAACAC TACGGAAGTG ATTTACGAAG CAATGAGACA AGTATTGGCT 
GCTCGGGCTA TTCATAACAT CTTCCGGATG ACAGAATCCC ATCTGATGGT CACCAGTCAG 
ACTCTGAGGT TGATAGATCC CCAGACTCAA GTAACAAGGG CCAATTTTGA ACTCACCAGT 
GTCACCCAGT TTGCTGCTCA TCAAGAAAAC AAGAGACTGG TTGGCTTTGT GGTCCGCTTG 
CCTGAATCCA CAGGCGGAGA GTCTCTGAGC ACATATGTTT TTGAAAGCAA CTCAGAAGGC 
GAAAAGATAT GTTATGCTAT TAATTTGGGA AAAGAAATCA TTGAGGTTCA GAAGGCACAC 
CACCTGGTTT CAGAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803467.1
CDS	219..2099
Translation	MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNVLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASSEHDLSMAKYSRLPKKKENEKVKAEIVKEVAAARRKQHLSSLQYYCALNALQYRKRVAMMEPMLGFAHGQINFFKKGAEMFSQSMDGFLSSVADMVQSIQVELEVEAEKMRASQQELLSLDEAVYTPDFDVAAPQVNRNLIQKAGYLNLRNKTGLVSTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKAGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFAKKQESPCPSQNVKSSEMENDKIVPKATVTIPEAEQLIAPGTPIQFDIVLPATEFLDQNRGGRRTNPFGETEDATFPEAEDSLLQQMFIVRFLGSMAVKTDNTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQTLRLIDPQTQVTRANFELTSVTQFAAHQENKRLVGFVVRLPESTGGESLSTYVFESNSEGEKICYAINLGKEIIEVQKAHHLVSEC

Target ORF information:

RefSeq Version	XM_010805165.2
Organism	Bos taurus(cattle)
Definition	Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805165.2

ORF Insert Sequence:

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GTGTGTTTGA GGAAGATGCC GGCACTCTCA CAGATTATAC CAACCAGCTG 
CTTCAAGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAAA TGTGCCTGGC TACTCAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAATTTTG CTCTTGGCAA AGGCGATGAA 
GAGGTCATTT CTACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TGTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CAATTATACA ATTTCGAGAA 
AAGGATCTTA CAGAAGTAAG CACTTTAAAG GATCTTTTCG GACTTGCCAG CAGCGAGCAT 
GACCTCTCAA TGGCAAAATA TAGCAGGCTT CCTAAAAAAA AGGAGAATGA AAAGGTGAAG 
GCTGAAATCG TGAAGGAGGT GGCCGCAGCC CGGCGAAAAC AGCACCTTTC CTCCCTTCAG 
TACTACTGTG CCCTCAATGC GCTGCAGTAC AGAAAGCGCG TGGCCATGAT GGAGCCCATG 
CTGGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCACAG 
AGTATGGACG GCTTCTTATC CTCCGTCGCA GACATGGTTC AAAGCATCCA GGTGGAACTA 
GAAGTGGAAG CAGAGAAGAT GAGGGCATCC CAGCAAGAAT TACTTTCTCT CGATGAAGCT 
GTTTACACCC CAGACTTCGA TGTGGCTGCA CCCCAGGTCA ACAGGAACCT CATCCAGAAG 
GCCGGTTACC TTAATCTTAG AAATAAAACA GGGCTGGTCT CAACCACCTG GGAGAGACTC 
TACTTCTTCA CCCAAGGCGG GAATCTCATG TGCCAGCCTA GGGGCGCCGT GGCTGGGGGC 
CTGATCCAGG ACCTGGACAA CTGCTCGGTG ATGGCGGTGG ACTGTGAGGA CCGACGATAC 
TGCTTCCAGA TCACCACTCC CAATGGAAAA GCGGGAATAA TCCTCCAGGC AGAAAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACT 
GACAACCCAG AGGCAGTCGC CATCAAGTTG AATCAGACGG CTCTCCAAGC AGTGACTCCA 
ATTACAAGCT TTGCAAAAAA ACAAGAAAGT CCATGCCCCA GCCAGAACGT GAAAAGTTCA 
GAGATGGAAA ATGACAAGAT AGTTCCGAAA GCAACAGTCA CTATACCCGA AGCTGAACAA 
CTGATTGCAC CTGGAACACC TATTCAGTTT GACATTGTAC TTCCTGCTAC AGAATTCCTT 
GATCAGAACA GAGGCGGCAG GCGTACCAAC CCCTTTGGTG AAACTGAGGA TGCGACATTT 
CCAGAAGCAG AAGATTCTCT TTTGCAGCAG ATGTTTATAG TTCGGTTTTT GGGATCAATG 
GCAGTCAAAA CAGACAACAC TACGGAAGTG ATTTACGAAG CAATGAGACA AGTATTGGCT 
GCTCGGGCTA TTCATAACAT CTTCCGGATG ACAGAATCCC ATCTGATGGT CACCAGTCAG 
ACTCTGAGGT TGATAGATCC CCAGACTCAA GTAACAAGGG CCAATTTTGA ACTCACCAGT 
GTCACCCAGT TTGCTGCTCA TCAAGAAAAC AAGAGACTGG TTGGCTTTGT GGTCCGCTTG 
CCTGAATCCA CAGGCGGAGA GTCTCTGAGC ACATATGTTT TTGAAAGCAA CTCAGAAGGC 
GAAAAGATAT GTTATGCTAT TAATTTGGGA AAAGAAATCA TTGAGGTTCA GAAGGCACAC 
CACCTGGTTT CAGAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_010805165.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803467.1 Bos taurus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa49348
Clone ID Related Accession (Same CDS sequence)	XM_010805165.2 , XM_010805165.1
Accession Version	XM_010805165.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DCC-interacting protein 13-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GTGTGTTTGA GGAAGATGCC GGCACTCTCA CAGATTATAC CAACCAGCTG 
CTTCAAGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAAA TGTGCCTGGC TACTCAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAATTTTG CTCTTGGCAA AGGCGATGAA 
GAGGTCATTT CTACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TGTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CAATTATACA ATTTCGAGAA 
AAGGATCTTA CAGAAGTAAG CACTTTAAAG GATCTTTTCG GACTTGCCAG CAGCGAGCAT 
GACCTCTCAA TGGCAAAATA TAGCAGGCTT CCTAAAAAAA AGGAGAATGA AAAGGTGAAG 
GCTGAAATCG TGAAGGAGGT GGCCGCAGCC CGGCGAAAAC AGCACCTTTC CTCCCTTCAG 
TACTACTGTG CCCTCAATGC GCTGCAGTAC AGAAAGCGCG TGGCCATGAT GGAGCCCATG 
CTGGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCACAG 
AGTATGGACG GCTTCTTATC CTCCGTCGCA GACATGGTTC AAAGCATCCA GGTGGAACTA 
GAAGTGGAAG CAGAGAAGAT GAGGGCATCC CAGCAAGAAT TACTTTCTCT CGATGAAGCT 
GTTTACACCC CAGACTTCGA TGTGGCTGCA CCCCAGGTCA ACAGGAACCT CATCCAGAAG 
GCCGGTTACC TTAATCTTAG AAATAAAACA GGGCTGGTCT CAACCACCTG GGAGAGACTC 
TACTTCTTCA CCCAAGGCGG GAATCTCATG TGCCAGCCTA GGGGCGCCGT GGCTGGGGGC 
CTGATCCAGG ACCTGGACAA CTGCTCGGTG ATGGCGGTGG ACTGTGAGGA CCGACGATAC 
TGCTTCCAGA TCACCACTCC CAATGGAAAA GCGGGAATAA TCCTCCAGGC AGAAAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACT 
GACAACCCAG AGGCAGTCGC CATCAAGTTG AATCAGACGG CTCTCCAAGC AGTGACTCCA 
ATTACAAGCT TTGCAAAAAA ACAAGAAAGT CCATGCCCCA GCCAGAACGT GAAAAGTTCA 
GAGATGGAAA ATGACAAGAT AGTTCCGAAA GCAACAGTCA CTATACCCGA AGCTGAACAA 
CTGATTGCAC CTGGAACACC TATTCAGTTT GACATTGTAC TTCCTGCTAC AGAATTCCTT 
GATCAGAACA GAGGCGGCAG GCGTACCAAC CCCTTTGGTG AAACTGAGGA TGCGACATTT 
CCAGAAGCAG AAGATTCTCT TTTGCAGCAG ATGTTTATAG TTCGGTTTTT GGGATCAATG 
GCAGTCAAAA CAGACAACAC TACGGAAGTG ATTTACGAAG CAATGAGACA AGTATTGGCT 
GCTCGGGCTA TTCATAACAT CTTCCGGATG ACAGAATCCC ATCTGATGGT CACCAGTCAG 
ACTCTGAGGT TGATAGATCC CCAGACTCAA GTAACAAGGG CCAATTTTGA ACTCACCAGT 
GTCACCCAGT TTGCTGCTCA TCAAGAAAAC AAGAGACTGG TTGGCTTTGT GGTCCGCTTG 
CCTGAATCCA CAGGCGGAGA GTCTCTGAGC ACATATGTTT TTGAAAGCAA CTCAGAAGGC 
GAAAAGATAT GTTATGCTAT TAATTTGGGA AAAGAAATCA TTGAGGTTCA GAAGGCACAC 
CACCTGGTTT CAGAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803467.1
CDS	224..2104
Translation	MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNVLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASSEHDLSMAKYSRLPKKKENEKVKAEIVKEVAAARRKQHLSSLQYYCALNALQYRKRVAMMEPMLGFAHGQINFFKKGAEMFSQSMDGFLSSVADMVQSIQVELEVEAEKMRASQQELLSLDEAVYTPDFDVAAPQVNRNLIQKAGYLNLRNKTGLVSTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKAGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFAKKQESPCPSQNVKSSEMENDKIVPKATVTIPEAEQLIAPGTPIQFDIVLPATEFLDQNRGGRRTNPFGETEDATFPEAEDSLLQQMFIVRFLGSMAVKTDNTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQTLRLIDPQTQVTRANFELTSVTQFAAHQENKRLVGFVVRLPESTGGESLSTYVFESNSEGEKICYAINLGKEIIEVQKAHHLVSEC

Target ORF information:

RefSeq Version	XM_010805165.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805165.1

ORF Insert Sequence:

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GTGTGTTTGA GGAAGATGCC GGCACTCTCA CAGATTATAC CAACCAGCTG 
CTTCAAGCAA TGCAGCGCGT CTATGGAGCC CAGAATGAAA TGTGCCTGGC TACTCAACAG 
CTTTCTAAGC AACTGCTGGC ATATGAAAAA CAGAATTTTG CTCTTGGCAA AGGCGATGAA 
GAGGTCATTT CTACACTCCA CTATTTTTCC AAAGTGGTGG ATGAGCTTAA TGTTCTCCAT 
ACAGAGCTGG CTAAACAGTT GGCAGACACA ATGGTTCTAC CAATTATACA ATTTCGAGAA 
AAGGATCTTA CAGAAGTAAG CACTTTAAAG GATCTTTTCG GACTTGCCAG CAGCGAGCAT 
GACCTCTCAA TGGCAAAATA TAGCAGGCTT CCTAAAAAAA AGGAGAATGA AAAGGTGAAG 
GCTGAAATCG TGAAGGAGGT GGCCGCAGCC CGGCGAAAAC AGCACCTTTC CTCCCTTCAG 
TACTACTGTG CCCTCAATGC GCTGCAGTAC AGAAAGCGCG TGGCCATGAT GGAGCCCATG 
CTGGGCTTTG CCCATGGACA GATTAACTTT TTTAAGAAGG GAGCAGAGAT GTTTTCACAG 
AGTATGGACG GCTTCTTATC CTCCGTCGCA GACATGGTTC AAAGCATCCA GGTGGAACTA 
GAAGTGGAAG CAGAGAAGAT GAGGGCATCC CAGCAAGAAT TACTTTCTCT CGATGAAGCT 
GTTTACACCC CAGACTTCGA TGTGGCTGCA CCCCAGGTCA ACAGGAACCT CATCCAGAAG 
GCCGGTTACC TTAATCTTAG AAATAAAACA GGGCTGGTCT CAACCACCTG GGAGAGACTC 
TACTTCTTCA CCCAAGGCGG GAATCTCATG TGCCAGCCTA GGGGCGCCGT GGCTGGGGGC 
CTGATCCAGG ACCTGGACAA CTGCTCGGTG ATGGCGGTGG ACTGTGAGGA CCGACGATAC 
TGCTTCCAGA TCACCACTCC CAATGGAAAA GCGGGAATAA TCCTCCAGGC AGAAAGCAGA 
AAGGAAAATG AAGAGTGGAT ATGTGCAATA AACAACATCT CCAGACAGAT CTACCTGACT 
GACAACCCAG AGGCAGTCGC CATCAAGTTG AATCAGACGG CTCTCCAAGC AGTGACTCCA 
ATTACAAGCT TTGCAAAAAA ACAAGAAAGT CCATGCCCCA GCCAGAACGT GAAAAGTTCA 
GAGATGGAAA ATGACAAGAT AGTTCCGAAA GCAACAGTCA CTATACCCGA AGCTGAACAA 
CTGATTGCAC CTGGAACACC TATTCAGTTT GACATTGTAC TTCCTGCTAC AGAATTCCTT 
GATCAGAACA GAGGCGGCAG GCGTACCAAC CCCTTTGGTG AAACTGAGGA TGCGACATTT 
CCAGAAGCAG AAGATTCTCT TTTGCAGCAG ATGTTTATAG TTCGGTTTTT GGGATCAATG 
GCAGTCAAAA CAGACAACAC TACGGAAGTG ATTTACGAAG CAATGAGACA AGTATTGGCT 
GCTCGGGCTA TTCATAACAT CTTCCGGATG ACAGAATCCC ATCTGATGGT CACCAGTCAG 
ACTCTGAGGT TGATAGATCC CCAGACTCAA GTAACAAGGG CCAATTTTGA ACTCACCAGT 
GTCACCCAGT TTGCTGCTCA TCAAGAAAAC AAGAGACTGG TTGGCTTTGT GGTCCGCTTG 
CCTGAATCCA CAGGCGGAGA GTCTCTGAGC ACATATGTTT TTGAAAGCAA CTCAGAAGGC 
GAAAAGATAT GTTATGCTAT TAATTTGGGA AAAGAAATCA TTGAGGTTCA GAAGGCACAC 
CACCTGGTTT CAGAATGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL2 ( XM_024991717.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847485.1 Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X3, mRNA.

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CloneID	OBa234361
Clone ID Related Accession (Same CDS sequence)	XM_024991717.1
Accession Version	XM_024991717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DCC-interacting protein 13-beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GTGTGTTTGA GGAAGATGCC GGCACTCTCA CAGATTATAC CAACCAGCTG 
CTTCAAGCAA TGCAGCGCGT CTATGGAGCC CAGCTTAATG TTCTCCATAC AGAGCTGGCT 
AAACAGTTGG CAGACACAAT GGTTCTACCA ATTATACAAT TTCGAGAAAA GGATCTTACA 
GAAGTAAGCA CTTTAAAGGA TCTTTTCGGA CTTGCCAGCA GCGAGCATGA CCTCTCAATG 
GCAAAATATA GCAGGCTTCC TAAAAAAAAG GAGAATGAAA AGGTGAAGGC TGAAATCGTG 
AAGGAGGTGG CCGCAGCCCG GCGAAAACAG CACCTTTCCT CCCTTCAGTA CTACTGTGCC 
CTCAATGCGC TGCAGTACAG AAAGCGCGTG GCCATGATGG AGCCCATGCT GGGCTTTGCC 
CATGGACAGA TTAACTTTTT TAAGAAGGGA GCAGAGATGT TTTCACAGAG TATGGACGGC 
TTCTTATCCT CCGTCGCAGA CATGGTTCAA AGCATCCAGG TGGAACTAGA AGTGGAAGCA 
GAGAAGATGA GGGCATCCCA GCAAGAATTA CTTTCTCTCG ATGAAGCTGT TTACACCCCA 
GACTTCGATG TGGCTGCACC CCAGGTCAAC AGGAACCTCA TCCAGAAGGC CGGTTACCTT 
AATCTTAGAA ATAAAACAGG GCTGGTCTCA ACCACCTGGG AGAGACTCTA CTTCTTCACC 
CAAGGCGGGA ATCTCATGTG CCAGCCTAGG GGCGCCGTGG CTGGGGGCCT GATCCAGGAC 
CTGGACAACT GCTCGGTGAT GGCGGTGGAC TGTGAGGACC GACGATACTG CTTCCAGATC 
ACCACTCCCA ATGGAAAAGC GGGAATAATC CTCCAGGCAG AAAGCAGAAA GGAAAATGAA 
GAGTGGATAT GTGCAATAAA CAACATCTCC AGACAGATCT ACCTGACTGA CAACCCAGAG 
GCAGTCGCCA TCAAGTTGAA TCAGACGGCT CTCCAAGCAG TGACTCCAAT TACAAGCTTT 
GCAAAAAAAC AAGAAAGTCC ATGCCCCAGC CAGAACGTGA AAAGTTCAGA GATGGAAAAT 
GACAAGATAG TTCCGAAAGC AACAGTCACT ATACCCGAAG CTGAACAACT GATTGCACCT 
GGAACACCTA TTCAGTTTGA CATTGTACTT CCTGCTACAG AATTCCTTGA TCAGAACAGA 
GGCGGCAGGC GTACCAACCC CTTTGGTGAA ACTGAGGATG CGACATTTCC AGAAGCAGAA 
GATTCTCTTT TGCAGCAGAT GTTTATAGTT CGGTTTTTGG GATCAATGGC AGTCAAAACA 
GACAACACTA CGGAAGTGAT TTACGAAGCA ATGAGACAAG TATTGGCTGC TCGGGCTATT 
CATAACATCT TCCGGATGAC AGAATCCCAT CTGATGGTCA CCAGTCAGAC TCTGAGGTTG 
ATAGATCCCC AGACTCAAGT AACAAGGGCC AATTTTGAAC TCACCAGTGT CACCCAGTTT 
GCTGCTCATC AAGAAAACAA GAGACTGGTT GGCTTTGTGG TCCGCTTGCC TGAATCCACA 
GGCGGAGAGT CTCTGAGCAC ATATGTTTTT GAAAGCAACT CAGAAGGCGA AAAGATATGT 
TATGCTATTA ATTTGGGAAA AGAAATCATT GAGGTTCAGA AGGATCCAGA AGCACTGGCT 
CAATTAATGC TGTCCGTACC ACTAACCAAT GATGGAAAAT ATGTACTATT AAACGATCAA 
CCAGATGACA ATGATGGAAG TCCAAGTGAA AATAGAGGCG CAGAATCTGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847485.1
CDS	94..1950
Translation	MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQLNVLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASSEHDLSMAKYSRLPKKKENEKVKAEIVKEVAAARRKQHLSSLQYYCALNALQYRKRVAMMEPMLGFAHGQINFFKKGAEMFSQSMDGFLSSVADMVQSIQVELEVEAEKMRASQQELLSLDEAVYTPDFDVAAPQVNRNLIQKAGYLNLRNKTGLVSTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKAGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFAKKQESPCPSQNVKSSEMENDKIVPKATVTIPEAEQLIAPGTPIQFDIVLPATEFLDQNRGGRRTNPFGETEDATFPEAEDSLLQQMFIVRFLGSMAVKTDNTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQTLRLIDPQTQVTRANFELTSVTQFAAHQENKRLVGFVVRLPESTGGESLSTYVFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSVPLTNDGKYVLLNDQPDDNDGSPSENRGAESEA

Target ORF information:

RefSeq Version	XM_024991717.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991717.1

ORF Insert Sequence:

ATGCCCGCTG TGGACAAGCT CTTGCTAGAG GAGGCGTTGC AGGACAGCCC CCAGACTCGC 
TCTTTACTGA GTGTGTTTGA GGAAGATGCC GGCACTCTCA CAGATTATAC CAACCAGCTG 
CTTCAAGCAA TGCAGCGCGT CTATGGAGCC CAGCTTAATG TTCTCCATAC AGAGCTGGCT 
AAACAGTTGG CAGACACAAT GGTTCTACCA ATTATACAAT TTCGAGAAAA GGATCTTACA 
GAAGTAAGCA CTTTAAAGGA TCTTTTCGGA CTTGCCAGCA GCGAGCATGA CCTCTCAATG 
GCAAAATATA GCAGGCTTCC TAAAAAAAAG GAGAATGAAA AGGTGAAGGC TGAAATCGTG 
AAGGAGGTGG CCGCAGCCCG GCGAAAACAG CACCTTTCCT CCCTTCAGTA CTACTGTGCC 
CTCAATGCGC TGCAGTACAG AAAGCGCGTG GCCATGATGG AGCCCATGCT GGGCTTTGCC 
CATGGACAGA TTAACTTTTT TAAGAAGGGA GCAGAGATGT TTTCACAGAG TATGGACGGC 
TTCTTATCCT CCGTCGCAGA CATGGTTCAA AGCATCCAGG TGGAACTAGA AGTGGAAGCA 
GAGAAGATGA GGGCATCCCA GCAAGAATTA CTTTCTCTCG ATGAAGCTGT TTACACCCCA 
GACTTCGATG TGGCTGCACC CCAGGTCAAC AGGAACCTCA TCCAGAAGGC CGGTTACCTT 
AATCTTAGAA ATAAAACAGG GCTGGTCTCA ACCACCTGGG AGAGACTCTA CTTCTTCACC 
CAAGGCGGGA ATCTCATGTG CCAGCCTAGG GGCGCCGTGG CTGGGGGCCT GATCCAGGAC 
CTGGACAACT GCTCGGTGAT GGCGGTGGAC TGTGAGGACC GACGATACTG CTTCCAGATC 
ACCACTCCCA ATGGAAAAGC GGGAATAATC CTCCAGGCAG AAAGCAGAAA GGAAAATGAA 
GAGTGGATAT GTGCAATAAA CAACATCTCC AGACAGATCT ACCTGACTGA CAACCCAGAG 
GCAGTCGCCA TCAAGTTGAA TCAGACGGCT CTCCAAGCAG TGACTCCAAT TACAAGCTTT 
GCAAAAAAAC AAGAAAGTCC ATGCCCCAGC CAGAACGTGA AAAGTTCAGA GATGGAAAAT 
GACAAGATAG TTCCGAAAGC AACAGTCACT ATACCCGAAG CTGAACAACT GATTGCACCT 
GGAACACCTA TTCAGTTTGA CATTGTACTT CCTGCTACAG AATTCCTTGA TCAGAACAGA 
GGCGGCAGGC GTACCAACCC CTTTGGTGAA ACTGAGGATG CGACATTTCC AGAAGCAGAA 
GATTCTCTTT TGCAGCAGAT GTTTATAGTT CGGTTTTTGG GATCAATGGC AGTCAAAACA 
GACAACACTA CGGAAGTGAT TTACGAAGCA ATGAGACAAG TATTGGCTGC TCGGGCTATT 
CATAACATCT TCCGGATGAC AGAATCCCAT CTGATGGTCA CCAGTCAGAC TCTGAGGTTG 
ATAGATCCCC AGACTCAAGT AACAAGGGCC AATTTTGAAC TCACCAGTGT CACCCAGTTT 
GCTGCTCATC AAGAAAACAA GAGACTGGTT GGCTTTGTGG TCCGCTTGCC TGAATCCACA 
GGCGGAGAGT CTCTGAGCAC ATATGTTTTT GAAAGCAACT CAGAAGGCGA AAAGATATGT 
TATGCTATTA ATTTGGGAAA AGAAATCATT GAGGTTCAGA AGGATCCAGA AGCACTGGCT 
CAATTAATGC TGTCCGTACC ACTAACCAAT GATGGAAAAT ATGTACTATT AAACGATCAA 
CCAGATGACA ATGATGGAAG TCCAAGTGAA AATAGAGGCG CAGAATCTGA AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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APPL2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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