SPG7 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPG7
Entrez Gene ID	515269
Full Name	SPG7, paraplegin matrix AAA peptidase subunit
General protein information	Preferred Names SPG7, paraplegin matrix AAA peptidase subunit Names paraplegin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001102022.1	NP_001095492.1	paraplegin
XM_015475740.2	XP_015331226.2	paraplegin isoform X1
XM_015475740.1	XP_015331226.1	paraplegin isoform X1
XM_024978210.1	XP_024833978.1	paraplegin isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPG7	XP_001140249.3
	SPG7	XP_002802648.1
Rattus norvegicus (Norway rat)	Spg7	NP_852053.1
Danio rerio (zebrafish)	spg7	XP_001923118.1
Gallus gallus (chicken)	SPG7	NP_001012545.1
Drosophila melanogaster (fruit fly)	CG2658	NP_570017.1
Caenorhabditis elegans (roundworm)	ppgn-1	NP_500191.3
Mus musculus (house mouse)	Spg7	NP_694816.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000560	XP_310523.5
Homo sapiens (human)	SPG7	NP_003110.1
Canis lupus familiaris (dog)	SPG7	XP_546777.3
Bos taurus (cattle)	SPG7	NP_001095492.1
Xenopus tropicalis (tropical clawed frog)	spg7	XP_002933705.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPG7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39968	NM_001102022.1 Latest version!	Bos taurus SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa224986	XM_015475740.2 Latest version!	Bos taurus SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa224987	XM_024978210.1 Latest version!	Bos taurus SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa93363	XM_015475740.1 Latest version!	Bos taurus spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPG7 ( NM_001102022.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095492.1 Bos taurus SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), mRNA.

Price & Availability↑

CloneID	OBa39968
Clone ID Related Accession (Same CDS sequence)	NM_001102022.1
Accession Version	NM_001102022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-31
Organism	Bos taurus(cattle)
Product	paraplegin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151632.1. On Aug 21, 2007 this sequence version replaced XM_593253.3. ##Evidence-Data-START## Transcript exon combination :: BC151632.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCCG CGTGGCTGCT GCTGCGCGCT CTCCGCCCGG GTCCGGGACC GGGTCCGGGG 
AGGCTGCGGT GCCCCTACTC GGGCTGGAGC CCAGGCTGCA CTTCCGGAGC CAAGTGGAGG 
TGGCCGAATG ACGTCCACAG GCCTCCGATG GGCCCCATCG GGCCTGGAGG CCGAGCCCTG 
CAGAGTTTCC AGTTGCGACT GCTAACCCCT ACCTTTGAAG GAATCAGCAG ATTGTTGGTG 
AAACAGCACA TTGTACAGAA TCCAGTTGGA CTCTGGAAAC TTCTAGGAGG CACTTACTAT 
TTTAACACTT CGAGGAGAAA GCAGAAGACC AAGGACAATG ATAAAGCCAA GGGGAGGAGC 
CCTGAGGATG ACGAAGAGGA GCGCAGGCGC AGGGAGCGCG AGGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCCTCTTCGT CATCGCAGTG ATCATGAGTC TGCTCAATGC GCTGGGCTCC 
AGTGGAGGCA ACATCTCCTG GAATGACTTC GTGCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGGGTGC AAGTGGTGCC CGAGAGCGAC GTGGTGGAGG TGTACCTGCA CCCCGGTGCC 
GTGGTGTTTG GGCGGCCTCG GCTGGCACTG ATGTACCGAA TGCAGGTGGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCTGAG GATGAGCTGA ATATCGAAGG CAAGGACAGG 
ATTCCAGTTT CCTACAAGCG GACGGGATTC TTTGGAAATG CCCTCTACGC CCTGGGGATG 
ACGGCCGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCTGGGAT GACAGGACGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTG AAAATGGCTC GTTTCACGAT TGTGGATGGC 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAT GTGGCAGGAA TGCATGAAGC CAAACTGGAG 
GTCAAGGAGT TCGTGGATTA TCTGAAGAGC CCGGAGCGCT TCCTTCAGCT TGGCGCCAAG 
GTCCCGAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGCG GGAAGACGCT GCTGGCCAAG 
GCAGTGGCCA CGGAGGCCCA GGTGCCCTTC CTGGCAATGG CTGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGTGC GGCCCGTGTG CGGAGCCTCT TCAAGGAAGC CCGGGCCCGG 
GCCCCCTGCA TCGTGTATAT CGACGAGATC GACGCCGTGG GCAAGAAGCG TTCCACCGCC 
GTGTCCGGCT TCTCCAACAC AGAGGAGGAG CAGACGCTCA ACCAGCTGCT GGTGGAGATG 
GACGGAATGG GTACCACCGA CCACGTCATC GTCCTGGCGG CCACCAACCG AGCTGACATT 
TTGGACAACG CTCTGCTAAG GCCGGGCCGA CTGGACAGGC ACGTTTTCAT CGACCTTCCC 
ACGCTGCAGG AAAGGAAGGA GATCTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACGCGG 
GCCAGCAGCT TTTACTCCCA GCGGCTGGCG GAGCTGACAC CTGGCTTCAG TGGCGCTGAC 
ATTGCCAACA TCTGTAACGA GGCCGCGTTG CACGCGGCGC GGGAGGGGCA CACGGCTGTG 
CACACATCCA ACCTGGACTA CGCCGTGGAG CGTGTCGTGG CCGGGACTGC CAAGAAGAGC 
AAAGTCCTTT CCAAGGAAGA GCAGAAGGTG GTTGCGTTCC ATGAGTCGGG CCACGCCCTG 
GTGGGCTGGC TGCTGGAGCA CACGGAGGCT GTGATGAAGG TGTCCATCAC TCCTCGGACC 
AATGCAGCCC TGGGCTTCGC TCAGATGCTC CCGAGAGACC AGCACCTCTT CACCAGGGAG 
CAGCTATTCG AGCGCATGTG CATGGCCCTG GGCGGCCGTG CATCCGAGTC CATCTCTTTC 
AACAGGGTCA CTTCCGGGGC ACAGGACGAC TTGCGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCACCCAGC ATCGGGCCTG TCTCCTTCCC CGAGGCCCAG 
GAGGGCGTCA CGGGCATCGG ACGGCGTCCA TTCAGCCAGG GCCTCCAGCA GATGATAGAC 
CACGAAGCGA GGCTGCTGGT GGCCACTGCC TATAGGCACA CGGAACAGGT GCTTCGGGAC 
AACCTGGACA AGCTCCACGC GCTGGCGAGT GCCCTGCTGG AAAAGGAAGT GATCAACTAC 
GAGGACATCG AGGCCCTCAT CGGCCCTCCG CCCCACGGGC CCAAGAAAAA GATCGCGCCG 
CAGAAGTGGA GCGATGCCCA GAGGGAGAAG CAGGACTCGG GGGAGGAGGA GGGAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095492.1
CDS	22..2361
Translation	MAAAWLLLRALRPGPGPGPGRLRCPYSGWSPGCTSGAKWRWPNDVHRPPMGPIGPGGRALQSFQLRLLTPTFEGISRLLVKQHIVQNPVGLWKLLGGTYYFNTSRRKQKTKDNDKAKGRSPEDDEEERRRREREDQMYRERLRTLFVIAVIMSLLNALGSSGGNISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEGKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVKEFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTAVSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLAATNRADILDNALLRPGRLDRHVFIDLPTLQERKEIFEQHLKSLKLTRASSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTAVHTSNLDYAVERVVAGTAKKSKVLSKEEQKVVAFHESGHALVGWLLEHTEAVMKVSITPRTNAALGFAQMLPRDQHLFTREQLFERMCMALGGRASESISFNRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGVTGIGRRPFSQGLQQMIDHEARLLVATAYRHTEQVLRDNLDKLHALASALLEKEVINYEDIEALIGPPPHGPKKKIAPQKWSDAQREKQDSGEEEGA

Target ORF information:

RefSeq Version	NM_001102022.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SPG7 matrix AAA peptidase subunit, paraplegin (SPG7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102022.1

ORF Insert Sequence:

ATGGCGGCCG CGTGGCTGCT GCTGCGCGCT CTCCGCCCGG GTCCGGGACC GGGTCCGGGG 
AGGCTGCGGT GCCCCTACTC GGGCTGGAGC CCAGGCTGCA CTTCCGGAGC CAAGTGGAGG 
TGGCCGAATG ACGTCCACAG GCCTCCGATG GGCCCCATCG GGCCTGGAGG CCGAGCCCTG 
CAGAGTTTCC AGTTGCGACT GCTAACCCCT ACCTTTGAAG GAATCAGCAG ATTGTTGGTG 
AAACAGCACA TTGTACAGAA TCCAGTTGGA CTCTGGAAAC TTCTAGGAGG CACTTACTAT 
TTTAACACTT CGAGGAGAAA GCAGAAGACC AAGGACAATG ATAAAGCCAA GGGGAGGAGC 
CCTGAGGATG ACGAAGAGGA GCGCAGGCGC AGGGAGCGCG AGGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCCTCTTCGT CATCGCAGTG ATCATGAGTC TGCTCAATGC GCTGGGCTCC 
AGTGGAGGCA ACATCTCCTG GAATGACTTC GTGCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGGGTGC AAGTGGTGCC CGAGAGCGAC GTGGTGGAGG TGTACCTGCA CCCCGGTGCC 
GTGGTGTTTG GGCGGCCTCG GCTGGCACTG ATGTACCGAA TGCAGGTGGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCTGAG GATGAGCTGA ATATCGAAGG CAAGGACAGG 
ATTCCAGTTT CCTACAAGCG GACGGGATTC TTTGGAAATG CCCTCTACGC CCTGGGGATG 
ACGGCCGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCTGGGAT GACAGGACGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTG AAAATGGCTC GTTTCACGAT TGTGGATGGC 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAT GTGGCAGGAA TGCATGAAGC CAAACTGGAG 
GTCAAGGAGT TCGTGGATTA TCTGAAGAGC CCGGAGCGCT TCCTTCAGCT TGGCGCCAAG 
GTCCCGAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGCG GGAAGACGCT GCTGGCCAAG 
GCAGTGGCCA CGGAGGCCCA GGTGCCCTTC CTGGCAATGG CTGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGTGC GGCCCGTGTG CGGAGCCTCT TCAAGGAAGC CCGGGCCCGG 
GCCCCCTGCA TCGTGTATAT CGACGAGATC GACGCCGTGG GCAAGAAGCG TTCCACCGCC 
GTGTCCGGCT TCTCCAACAC AGAGGAGGAG CAGACGCTCA ACCAGCTGCT GGTGGAGATG 
GACGGAATGG GTACCACCGA CCACGTCATC GTCCTGGCGG CCACCAACCG AGCTGACATT 
TTGGACAACG CTCTGCTAAG GCCGGGCCGA CTGGACAGGC ACGTTTTCAT CGACCTTCCC 
ACGCTGCAGG AAAGGAAGGA GATCTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACGCGG 
GCCAGCAGCT TTTACTCCCA GCGGCTGGCG GAGCTGACAC CTGGCTTCAG TGGCGCTGAC 
ATTGCCAACA TCTGTAACGA GGCCGCGTTG CACGCGGCGC GGGAGGGGCA CACGGCTGTG 
CACACATCCA ACCTGGACTA CGCCGTGGAG CGTGTCGTGG CCGGGACTGC CAAGAAGAGC 
AAAGTCCTTT CCAAGGAAGA GCAGAAGGTG GTTGCGTTCC ATGAGTCGGG CCACGCCCTG 
GTGGGCTGGC TGCTGGAGCA CACGGAGGCT GTGATGAAGG TGTCCATCAC TCCTCGGACC 
AATGCAGCCC TGGGCTTCGC TCAGATGCTC CCGAGAGACC AGCACCTCTT CACCAGGGAG 
CAGCTATTCG AGCGCATGTG CATGGCCCTG GGCGGCCGTG CATCCGAGTC CATCTCTTTC 
AACAGGGTCA CTTCCGGGGC ACAGGACGAC TTGCGGAAGG TCACCCGCAT CGCCTACTCC 
ATGGTGAAGC AGTTTGGGAT GGCACCCAGC ATCGGGCCTG TCTCCTTCCC CGAGGCCCAG 
GAGGGCGTCA CGGGCATCGG ACGGCGTCCA TTCAGCCAGG GCCTCCAGCA GATGATAGAC 
CACGAAGCGA GGCTGCTGGT GGCCACTGCC TATAGGCACA CGGAACAGGT GCTTCGGGAC 
AACCTGGACA AGCTCCACGC GCTGGCGAGT GCCCTGCTGG AAAAGGAAGT GATCAACTAC 
GAGGACATCG AGGCCCTCAT CGGCCCTCCG CCCCACGGGC CCAAGAAAAA GATCGCGCCG 
CAGAAGTGGA GCGATGCCCA GAGGGAGAAG CAGGACTCGG GGGAGGAGGA GGGAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG7 ( XM_015475740.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015331226.2 Bos taurus SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa224986
Clone ID Related Accession (Same CDS sequence)	XM_015475740.2
Accession Version	XM_015475740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	paraplegin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CGTGGCTGCT GCTGCGCGCT CTCCGCCCGG GTCCGGGACC GGGTCCGGGG 
AGGCTGCGGT GCCCCTACTC GGGCTGGAGC CCAGGCTGCA CTTCCGGAGC CAAGTGGAGG 
TGGCCGAATG ACGTCCACAG GCCTCCGATG GGCCCCATCG GGCCTGGAGG CCGAGCCCTG 
CAGAGTTTCC AGTTGCGACT GCTAACCCCT ACCTTTGAAG GAATCAGCAG ATTGTTGGTG 
AAACAGCACA TTGTACAGAA TCCAGTTGGA CTCTGGAAAC TTCTAGGAGG CACTTACTAT 
TTTAACACTT CGAGGAGAAA GCAGAAGACC AAGGACAATG ATAAAGCCAA GGGGAGGAGC 
CCTGAGGATG ACGAAGAGGA GCGCAGGCGC AGGGAGCGCG AGGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCCTCTTCGT CATCGCAGTG ATCATGAGTC TGCTCAATGC GCTGGGCTCC 
AGTGGAGGCA ACATCTCCTG GAATGACTTC GTGCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGGGTGC AAGTGGTGCC CGAGAGCGAC GTGGTGGAGG TGTACCTGCA CCCCGGTGCC 
GTGGTGTTTG GGCGGCCTCG GCTGGCACTG ATGTACCGAA TGCAGGTGGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCTGAG GATGAGCTGA ATATCGAAGG CAAGGACAGG 
ATTCCAGTTT CCTACAAGCG GACGGGATTC TTTGGAAATG CCCTCTACGC CCTGGGGATG 
ACGGCCGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCTGGGAT GACAGGACGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTG AAAATGGCTC GTTTCACGAT TGTGGATGGC 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAT GTGGCAGGAA TGCATGAAGC CAAACTGGAG 
GTCAAGGAGT TCGTGGATTA TCTGAAGAGC CCGGAGCGCT TCCTTCAGCT TGGCGCCAAG 
GTCCCGAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGCG GGAAGACGCT GCTGGCCAAG 
GCAGTGGCCA CGGAGGCCCA GGTGCCCTTC CTGGCAATGG CTGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGTGC GGCCCGTGTG CGGAGCCTCT TCAAGGAAGC CCGGGCCCGG 
GCCCCCTGCA TCGTGTATAT CGACGAGATC GACGCCGTGG GCAAGAAGCG TTCCACCGCC 
GTGTCCGGCT TCTCCAACAC AGAGGAGGAG CAGACGCTCA ACCAGCTGCT GGTGGAGATG 
GACGGAATGG GTACCACCGA CCACGTCATC GTCCTGGCGG CCACCAACCG AGCTGACATT 
TTGGACAACG CTCTGCTAAG GCCGGGCCGA CTGGACAGGC ACGTTTTCAT CGACCTTCCC 
ACGCTGCAGG AAAGGAAGGA GATCTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACGCGG 
GCCAGCAGCT TTTACTCCCA GCGGCTGGCG GAGCTGACAC CTGGCTTCAG TGGCGCTGAC 
ATTGCCAACA TCTGTAACGA GGCCGCGTTG CACGCGGCGC GGGAGGGGCA CACGGCTGTG 
CACACATCCA ACCTGGACTA CGCCGTGGAG CGTGTCGTGG CCGGGACTGC CAAGAAGAGC 
AAAGTCCTTT CCAAGGAAGA GCAGAAGGTG GTTGCGTTCC ATGAGTCGGG CCACGCCCTG 
GTGGGCTGGC TGCTGGAGCA CACGGAGGCT GTGATGAAGC CTGGGTCCTG CCGGCTAACA 
GACCACCTCA CTGTGCAGGT GTCCATCACT CCTCGGACCA ATGCAGCCCT GGGCTTCGCT 
CAGATGCTCC CGAGAGACCA GCACCTCTTC ACCAGGGAGC AGCTATTCGA GCGCATGTGC 
ATGGCCCTGG GCGGCCGTGC ATCCGAGTCC ATCTCTTTCA ACAGGGTCAC TTCCGGGGCA 
CAGGACGACT TGCGGAAGGT CACCCGCATC GCCTACTCCA TGGTGAAGCA GTTTGGGATG 
GCACCCAGCA TCGGGCCTGT CTCCTTCCCC GAGGCCCAGG AGGGCGTCAC GGGCATCGGA 
CGGCGTCCAT TCAGCCAGGG CCTCCAGCAG ATGATAGACC ACGAAGCGAG GCTGCTGGTG 
GCCACTGCCT ATAGGCACAC GGAACAGGTG CTTCGGGACA ACCTGGACAA GCTCCACGCG 
CTGGCGAGTG CCCTGCTGGA AAAGGAAGTG ATCAACTACG AGGACATCGA GGCCCTCATC 
GGCCCTCCGC CCCACGGGCC CAAGAAAAAG ATCGCGCCGC AGAAGTGGAG CGATGCCCAG 
AGGGAGAAGC AGGACTCGGG GGAGGAGGAG GGAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015331226.2
CDS	36..2414
Translation	MAAAWLLLRALRPGPGPGPGRLRCPYSGWSPGCTSGAKWRWPNDVHRPPMGPIGPGGRALQSFQLRLLTPTFEGISRLLVKQHIVQNPVGLWKLLGGTYYFNTSRRKQKTKDNDKAKGRSPEDDEEERRRREREDQMYRERLRTLFVIAVIMSLLNALGSSGGNISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEGKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVKEFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTAVSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLAATNRADILDNALLRPGRLDRHVFIDLPTLQERKEIFEQHLKSLKLTRASSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTAVHTSNLDYAVERVVAGTAKKSKVLSKEEQKVVAFHESGHALVGWLLEHTEAVMKPGSCRLTDHLTVQVSITPRTNAALGFAQMLPRDQHLFTREQLFERMCMALGGRASESISFNRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGVTGIGRRPFSQGLQQMIDHEARLLVATAYRHTEQVLRDNLDKLHALASALLEKEVINYEDIEALIGPPPHGPKKKIAPQKWSDAQREKQDSGEEEGA

Target ORF information:

RefSeq Version	XM_015475740.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475740.2

ORF Insert Sequence:

ATGGCGGCCG CGTGGCTGCT GCTGCGCGCT CTCCGCCCGG GTCCGGGACC GGGTCCGGGG 
AGGCTGCGGT GCCCCTACTC GGGCTGGAGC CCAGGCTGCA CTTCCGGAGC CAAGTGGAGG 
TGGCCGAATG ACGTCCACAG GCCTCCGATG GGCCCCATCG GGCCTGGAGG CCGAGCCCTG 
CAGAGTTTCC AGTTGCGACT GCTAACCCCT ACCTTTGAAG GAATCAGCAG ATTGTTGGTG 
AAACAGCACA TTGTACAGAA TCCAGTTGGA CTCTGGAAAC TTCTAGGAGG CACTTACTAT 
TTTAACACTT CGAGGAGAAA GCAGAAGACC AAGGACAATG ATAAAGCCAA GGGGAGGAGC 
CCTGAGGATG ACGAAGAGGA GCGCAGGCGC AGGGAGCGCG AGGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCCTCTTCGT CATCGCAGTG ATCATGAGTC TGCTCAATGC GCTGGGCTCC 
AGTGGAGGCA ACATCTCCTG GAATGACTTC GTGCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGGGTGC AAGTGGTGCC CGAGAGCGAC GTGGTGGAGG TGTACCTGCA CCCCGGTGCC 
GTGGTGTTTG GGCGGCCTCG GCTGGCACTG ATGTACCGAA TGCAGGTGGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCTGAG GATGAGCTGA ATATCGAAGG CAAGGACAGG 
ATTCCAGTTT CCTACAAGCG GACGGGATTC TTTGGAAATG CCCTCTACGC CCTGGGGATG 
ACGGCCGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCTGGGAT GACAGGACGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTG AAAATGGCTC GTTTCACGAT TGTGGATGGC 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAT GTGGCAGGAA TGCATGAAGC CAAACTGGAG 
GTCAAGGAGT TCGTGGATTA TCTGAAGAGC CCGGAGCGCT TCCTTCAGCT TGGCGCCAAG 
GTCCCGAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGCG GGAAGACGCT GCTGGCCAAG 
GCAGTGGCCA CGGAGGCCCA GGTGCCCTTC CTGGCAATGG CTGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGTGC GGCCCGTGTG CGGAGCCTCT TCAAGGAAGC CCGGGCCCGG 
GCCCCCTGCA TCGTGTATAT CGACGAGATC GACGCCGTGG GCAAGAAGCG TTCCACCGCC 
GTGTCCGGCT TCTCCAACAC AGAGGAGGAG CAGACGCTCA ACCAGCTGCT GGTGGAGATG 
GACGGAATGG GTACCACCGA CCACGTCATC GTCCTGGCGG CCACCAACCG AGCTGACATT 
TTGGACAACG CTCTGCTAAG GCCGGGCCGA CTGGACAGGC ACGTTTTCAT CGACCTTCCC 
ACGCTGCAGG AAAGGAAGGA GATCTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACGCGG 
GCCAGCAGCT TTTACTCCCA GCGGCTGGCG GAGCTGACAC CTGGCTTCAG TGGCGCTGAC 
ATTGCCAACA TCTGTAACGA GGCCGCGTTG CACGCGGCGC GGGAGGGGCA CACGGCTGTG 
CACACATCCA ACCTGGACTA CGCCGTGGAG CGTGTCGTGG CCGGGACTGC CAAGAAGAGC 
AAAGTCCTTT CCAAGGAAGA GCAGAAGGTG GTTGCGTTCC ATGAGTCGGG CCACGCCCTG 
GTGGGCTGGC TGCTGGAGCA CACGGAGGCT GTGATGAAGC CTGGGTCCTG CCGGCTAACA 
GACCACCTCA CTGTGCAGGT GTCCATCACT CCTCGGACCA ATGCAGCCCT GGGCTTCGCT 
CAGATGCTCC CGAGAGACCA GCACCTCTTC ACCAGGGAGC AGCTATTCGA GCGCATGTGC 
ATGGCCCTGG GCGGCCGTGC ATCCGAGTCC ATCTCTTTCA ACAGGGTCAC TTCCGGGGCA 
CAGGACGACT TGCGGAAGGT CACCCGCATC GCCTACTCCA TGGTGAAGCA GTTTGGGATG 
GCACCCAGCA TCGGGCCTGT CTCCTTCCCC GAGGCCCAGG AGGGCGTCAC GGGCATCGGA 
CGGCGTCCAT TCAGCCAGGG CCTCCAGCAG ATGATAGACC ACGAAGCGAG GCTGCTGGTG 
GCCACTGCCT ATAGGCACAC GGAACAGGTG CTTCGGGACA ACCTGGACAA GCTCCACGCG 
CTGGCGAGTG CCCTGCTGGA AAAGGAAGTG ATCAACTACG AGGACATCGA GGCCCTCATC 
GGCCCTCCGC CCCACGGGCC CAAGAAAAAG ATCGCGCCGC AGAAGTGGAG CGATGCCCAG 
AGGGAGAAGC AGGACTCGGG GGAGGAGGAG GGAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG7 ( XM_024978210.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833978.1 Bos taurus SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X3, mRNA.

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CloneID	OBa224987
Clone ID Related Accession (Same CDS sequence)	XM_024978210.1
Accession Version	XM_024978210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	paraplegin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGAG AGCGGCTGCG CACCCTCTTC GTCATCGCAG TGATCATGAG TCTGCTCAAT 
GCGCTGGGCT CCAGTGGAGG CAACATCTCC TGGAATGACT TCGTGCACGA GATGCTGGCC 
AAGGGCGAGG TGCAGCGGGT GCAAGTGGTG CCCGAGAGCG ACGTGGTGGA GGTGTACCTG 
CACCCCGGTG CCGTGGTGTT TGGGCGGCCT CGGCTGGCAC TGATGTACCG AATGCAGGTG 
GCAAACATCG ACAAATTTGA AGAGAAGCTT CGAGCAGCTG AGGATGAGCT GAATATCGAA 
GGCAAGGACA GGATTCCAGT TTCCTACAAG CGGACGGGAT TCTTTGGAAA TGCCCTCTAC 
GCCCTGGGGA TGACGGCCGT GGGCCTGGCC ATCCTGTGGT ATGTTTTCCG TCTGGCTGGG 
ATGACAGGAC GGGAAGGCGG ATTCAGTGCT TTTAATCAGC TGAAAATGGC TCGTTTCACG 
ATTGTGGATG GCAAGATGGG GAAAGGAGTC AGCTTCAAAG ATGTGGCAGG AATGCATGAA 
GCCAAACTGG AGGTCAAGGA GTTCGTGGAT TATCTGAAGA GCCCGGAGCG CTTCCTTCAG 
CTTGGCGCCA AGGTCCCGAA GGGCGCACTG CTGCTCGGCC CCCCCGGCTG CGGGAAGACG 
CTGCTGGCCA AGGCAGTGGC CACGGAGGCC CAGGTGCCCT TCCTGGCAAT GGCTGGCCCA 
GAGTTCGTGG AGGTCATTGG AGGCCTCGGT GCGGCCCGTG TGCGGAGCCT CTTCAAGGAA 
GCCCGGGCCC GGGCCCCCTG CATCGTGTAT ATCGACGAGA TCGACGCCGT GGGCAAGAAG 
CGTTCCACCG CCGTGTCCGG CTTCTCCAAC ACAGAGGAGG AGCAGACGCT CAACCAGCTG 
CTGGTGGAGA TGGACGGAAT GGGTACCACC GACCACGTCA TCGTCCTGGC GGCCACCAAC 
CGAGCTGACA TTTTGGACAA CGCTCTGCTA AGGCCGGGCC GACTGGACAG GCACGTTTTC 
ATCGACCTTC CCACGCTGCA GGAAAGGAAG GAGATCTTTG AGCAGCACCT GAAGAGCCTG 
AAGCTGACGC GGGCCAGCAG CTTTTACTCC CAGCGGCTGG CGGAGCTGAC ACCTGGCTTC 
AGTGGCGCTG ACATTGCCAA CATCTGTAAC GAGGCCGCGT TGCACGCGGC GCGGGAGGGG 
CACACGGCTG TGCACACATC CAACCTGGAC TACGCCGTGG AGCGTGTCGT GGCCGGGACT 
GCCAAGAAGA GCAAAGTCCT TTCCAAGGAA GAGCAGAAGG TGGTTGCGTT CCATGAGTCG 
GGCCACGCCC TGGTGGGCTG GCTGCTGGAG CACACGGAGG CTGTGATGAA GCCTGGGTCC 
TGCCGGCTAA CAGACCACCT CACTGTGCAG GTGTCCATCA CTCCTCGGAC CAATGCAGCC 
CTGGGCTTCG CTCAGATGCT CCCGAGAGAC CAGCACCTCT TCACCAGGGA GCAGCTATTC 
GAGCGCATGT GCATGGCCCT GGGCGGCCGT GCATCCGAGT CCATCTCTTT CAACAGGGTC 
ACTTCCGGGG CACAGGACGA CTTGCGGAAG GTCACCCGCA TCGCCTACTC CATGGTGAAG 
CAGTTTGGGA TGGCACCCAG CATCGGGCCT GTCTCCTTCC CCGAGGCCCA GGAGGGCGTC 
ACGGGCATCG GACGGCGTCC ATTCAGCCAG GGCCTCCAGC AGATGATAGA CCACGAAGCG 
AGGCTGCTGG TGGCCACTGC CTATAGGCAC ACGGAACAGG TGCTTCGGGA CAACCTGGAC 
AAGCTCCACG CGCTGGCGAG TGCCCTGCTG GAAAAGGAAG TGATCAACTA CGAGGACATC 
GAGGCCCTCA TCGGCCCTCC GCCCCACGGG CCCAAGAAAA AGATCGCGCC GCAGAAGTGG 
AGCGATGCCC AGAGGGAGAA GCAGGACTCG GGGGAGGAGG AGGGAGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833978.1
CDS	263..2233
Translation	MYRERLRTLFVIAVIMSLLNALGSSGGNISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEGKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVKEFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTAVSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLAATNRADILDNALLRPGRLDRHVFIDLPTLQERKEIFEQHLKSLKLTRASSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTAVHTSNLDYAVERVVAGTAKKSKVLSKEEQKVVAFHESGHALVGWLLEHTEAVMKPGSCRLTDHLTVQVSITPRTNAALGFAQMLPRDQHLFTREQLFERMCMALGGRASESISFNRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGVTGIGRRPFSQGLQQMIDHEARLLVATAYRHTEQVLRDNLDKLHALASALLEKEVINYEDIEALIGPPPHGPKKKIAPQKWSDAQREKQDSGEEEGA

Target ORF information:

RefSeq Version	XM_024978210.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978210.1

ORF Insert Sequence:

ATGTACCGAG AGCGGCTGCG CACCCTCTTC GTCATCGCAG TGATCATGAG TCTGCTCAAT 
GCGCTGGGCT CCAGTGGAGG CAACATCTCC TGGAATGACT TCGTGCACGA GATGCTGGCC 
AAGGGCGAGG TGCAGCGGGT GCAAGTGGTG CCCGAGAGCG ACGTGGTGGA GGTGTACCTG 
CACCCCGGTG CCGTGGTGTT TGGGCGGCCT CGGCTGGCAC TGATGTACCG AATGCAGGTG 
GCAAACATCG ACAAATTTGA AGAGAAGCTT CGAGCAGCTG AGGATGAGCT GAATATCGAA 
GGCAAGGACA GGATTCCAGT TTCCTACAAG CGGACGGGAT TCTTTGGAAA TGCCCTCTAC 
GCCCTGGGGA TGACGGCCGT GGGCCTGGCC ATCCTGTGGT ATGTTTTCCG TCTGGCTGGG 
ATGACAGGAC GGGAAGGCGG ATTCAGTGCT TTTAATCAGC TGAAAATGGC TCGTTTCACG 
ATTGTGGATG GCAAGATGGG GAAAGGAGTC AGCTTCAAAG ATGTGGCAGG AATGCATGAA 
GCCAAACTGG AGGTCAAGGA GTTCGTGGAT TATCTGAAGA GCCCGGAGCG CTTCCTTCAG 
CTTGGCGCCA AGGTCCCGAA GGGCGCACTG CTGCTCGGCC CCCCCGGCTG CGGGAAGACG 
CTGCTGGCCA AGGCAGTGGC CACGGAGGCC CAGGTGCCCT TCCTGGCAAT GGCTGGCCCA 
GAGTTCGTGG AGGTCATTGG AGGCCTCGGT GCGGCCCGTG TGCGGAGCCT CTTCAAGGAA 
GCCCGGGCCC GGGCCCCCTG CATCGTGTAT ATCGACGAGA TCGACGCCGT GGGCAAGAAG 
CGTTCCACCG CCGTGTCCGG CTTCTCCAAC ACAGAGGAGG AGCAGACGCT CAACCAGCTG 
CTGGTGGAGA TGGACGGAAT GGGTACCACC GACCACGTCA TCGTCCTGGC GGCCACCAAC 
CGAGCTGACA TTTTGGACAA CGCTCTGCTA AGGCCGGGCC GACTGGACAG GCACGTTTTC 
ATCGACCTTC CCACGCTGCA GGAAAGGAAG GAGATCTTTG AGCAGCACCT GAAGAGCCTG 
AAGCTGACGC GGGCCAGCAG CTTTTACTCC CAGCGGCTGG CGGAGCTGAC ACCTGGCTTC 
AGTGGCGCTG ACATTGCCAA CATCTGTAAC GAGGCCGCGT TGCACGCGGC GCGGGAGGGG 
CACACGGCTG TGCACACATC CAACCTGGAC TACGCCGTGG AGCGTGTCGT GGCCGGGACT 
GCCAAGAAGA GCAAAGTCCT TTCCAAGGAA GAGCAGAAGG TGGTTGCGTT CCATGAGTCG 
GGCCACGCCC TGGTGGGCTG GCTGCTGGAG CACACGGAGG CTGTGATGAA GCCTGGGTCC 
TGCCGGCTAA CAGACCACCT CACTGTGCAG GTGTCCATCA CTCCTCGGAC CAATGCAGCC 
CTGGGCTTCG CTCAGATGCT CCCGAGAGAC CAGCACCTCT TCACCAGGGA GCAGCTATTC 
GAGCGCATGT GCATGGCCCT GGGCGGCCGT GCATCCGAGT CCATCTCTTT CAACAGGGTC 
ACTTCCGGGG CACAGGACGA CTTGCGGAAG GTCACCCGCA TCGCCTACTC CATGGTGAAG 
CAGTTTGGGA TGGCACCCAG CATCGGGCCT GTCTCCTTCC CCGAGGCCCA GGAGGGCGTC 
ACGGGCATCG GACGGCGTCC ATTCAGCCAG GGCCTCCAGC AGATGATAGA CCACGAAGCG 
AGGCTGCTGG TGGCCACTGC CTATAGGCAC ACGGAACAGG TGCTTCGGGA CAACCTGGAC 
AAGCTCCACG CGCTGGCGAG TGCCCTGCTG GAAAAGGAAG TGATCAACTA CGAGGACATC 
GAGGCCCTCA TCGGCCCTCC GCCCCACGGG CCCAAGAAAA AGATCGCGCC GCAGAAGTGG 
AGCGATGCCC AGAGGGAGAA GCAGGACTCG GGGGAGGAGG AGGGAGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG7 ( XM_015475740.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015331226.1 Bos taurus spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa93363
Clone ID Related Accession (Same CDS sequence)	XM_015475740.1
Accession Version	XM_015475740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	paraplegin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG CGTGGCTGCT GCTGCGCGCT CTCCGCCCGG GTCCGGGACC GGGTCCGGGG 
AGGCTGCGGT GCCCCTACTC GGGCTGGAGC CCAGGCTGCA CTTCCGGAGC CAAGTGGAGG 
TGGCCGAATG ACGTCCACAG GCCTCCGATG GGCCCCATCG GGCCTGGAGG CCGAGCCCTG 
CAGAGTTTCC AGTTGCGACT GCTAACCCCT ACCTTTGAAG GAATCAGCAG ATTGTTGGTG 
AAACAGCACA TTGTACAGAA TCCAGTTGGA CTCTGGAAAC TTCTAGGAGG CACTTACTAT 
TTTAACACTT CGAGGAGAAA GCAGAAGACC AAGGACAATG ATAAAGCCAA GGGGAGGAGC 
CCTGAGGATG ACGAAGAGGA GCGCAGGCGC AGGGAGCGCG AGGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCCTCTTCGT CATCGCAGTG ATCATGAGTC TGCTCAATGC GCTGGGCTCC 
AGTGGAGGCA ACATCTCCTG GAATGACTTC GTGCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGGGTGC AAGTGGTGCC CGAGAGCGAC GTGGTGGAGG TGTACCTGCA CCCCGGTGCC 
GTGGTGTTTG GGCGGCCTCG GCTGGCACTG ATGTACCGAA TGCAGGTGGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCTGAG GATGAGCTGA ATATCGAAGG CAAGGACAGG 
ATTCCAGTTT CCTACAAGCG GACGGGATTC TTTGGAAATG CCCTCTACGC CCTGGGGATG 
ACGGCCGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCTGGGAT GACAGGACGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTG AAAATGGCTC GTTTCACGAT TGTGGATGGC 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAT GTGGCAGGAA TGCATGAAGC CAAACTGGAG 
GTCAAGGAGT TCGTGGATTA TCTGAAGAGC CCGGAGCGCT TCCTTCAGCT TGGCGCCAAG 
GTCCCGAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGCG GGAAGACGCT GCTGGCCAAG 
GCAGTGGCCA CGGAGGCCCA GGTGCCCTTC CTGGCAATGG CTGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGTGC GGCCCGTGTG CGGAGCCTCT TCAAGGAAGC CCGGGCCCGG 
GCCCCCTGCA TCGTGTATAT CGACGAGATC GACGCCGTGG GCAAGAAGCG TTCCACCGCC 
GTGTCCGGCT TCTCCAACAC AGAGGAGGAG CAGACGCTCA ACCAGCTGCT GGTGGAGATG 
GACGGAATGG GTACCACCGA CCACGTCATC GTCCTGGCGG CCACCAACCG AGCTGACATT 
TTGGACAACG CTCTGCTAAG GCCGGGCCGA CTGGACAGGC ACGTTTTCAT CGACCTTCCC 
ACGCTGCAGG AAAGGAAGGA GATCTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACGCGG 
GCCAGCAGCT TTTACTCCCA GCGGCTGGCG GAGCTGACAC CTGGCTTCAG TGGCGCTGAC 
ATTGCCAACA TCTGTAACGA GGCCGCGTTG CACGCGGCGC GGGAGGGGCA CACGGCTGTG 
CACACATCCA ACCTGGACTA CGCCGTGGAG CGTGTCGTGG CCGGGACTGC CAAGAAGAGC 
AAAGTCCTTT CCAAGGAAGA GCAGAAGGTG GTTGCGTTCC ATGAGTCGGG CCACGCCCTG 
GTGGGCTGGC TGCTGGAGCA CACGGAGGCT GTGATGAAGC CTGGGTCCTG CCAGCTAACA 
GACCACCTCA CTGTGCAGGT GTCCATCACT CCTCGGACCA ATGCAGCCCT GGGCTTCGCT 
CAGATGCTCC CGAGAGACCA GCACCTCTTC ACCAGGGAGC AGCTATTCGA GCGCATGTGC 
ATGGCCCTGG GCGGCCGTGC ATCCGAGTCC ATCTCTTTCA ACAGGGTCAC TTCCGGGGCA 
CAGGACGACT TGCGGAAGGT CACCCGCATC GCCTACTCCA TGGTGAAGCA GTTTGGGATG 
GCACCCAGCA TCGGGCCTGT CTCCTTCCCC GAGGCCCAGG AGGGCGTCAC GGGCATCGGA 
CGGCGTCCAT TCAGCCAGGG CCTCCAGCAG ATGATAGACC ACGAAGCGAG GCTGCTGGTG 
GCCACTGCCT ATAGGCACAC GGAACAGGTG CTTCGGGACA ACCTGGACAA GCTCCACGCG 
CTGGCGAGTG CCCTGCTGGA AAAGGAAGTG ATCAACTACG AGGACATCGA GGCCCTCATC 
GGCCCTCCGC CCCACGGGCC CAAGAAAAAG ATCGCGCCGC AGAAGTGGAG CGATGCCCAG 
AGGGAGAAGC AGGACTCGGG GGAGGAGGAG GGAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015331226.1
CDS	37..2415
Translation	MAAAWLLLRALRPGPGPGPGRLRCPYSGWSPGCTSGAKWRWPNDVHRPPMGPIGPGGRALQSFQLRLLTPTFEGISRLLVKQHIVQNPVGLWKLLGGTYYFNTSRRKQKTKDNDKAKGRSPEDDEEERRRREREDQMYRERLRTLFVIAVIMSLLNALGSSGGNISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEGKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVKEFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTAVSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLAATNRADILDNALLRPGRLDRHVFIDLPTLQERKEIFEQHLKSLKLTRASSFYSQRLAELTPGFSGADIANICNEAALHAAREGHTAVHTSNLDYAVERVVAGTAKKSKVLSKEEQKVVAFHESGHALVGWLLEHTEAVMKPGSCQLTDHLTVQVSITPRTNAALGFAQMLPRDQHLFTREQLFERMCMALGGRASESISFNRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEGVTGIGRRPFSQGLQQMIDHEARLLVATAYRHTEQVLRDNLDKLHALASALLEKEVINYEDIEALIGPPPHGPKKKIAPQKWSDAQREKQDSGEEEGA

Target ORF information:

RefSeq Version	XM_015475740.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475740.1

ORF Insert Sequence:

ATGGCGGCCG CGTGGCTGCT GCTGCGCGCT CTCCGCCCGG GTCCGGGACC GGGTCCGGGG 
AGGCTGCGGT GCCCCTACTC GGGCTGGAGC CCAGGCTGCA CTTCCGGAGC CAAGTGGAGG 
TGGCCGAATG ACGTCCACAG GCCTCCGATG GGCCCCATCG GGCCTGGAGG CCGAGCCCTG 
CAGAGTTTCC AGTTGCGACT GCTAACCCCT ACCTTTGAAG GAATCAGCAG ATTGTTGGTG 
AAACAGCACA TTGTACAGAA TCCAGTTGGA CTCTGGAAAC TTCTAGGAGG CACTTACTAT 
TTTAACACTT CGAGGAGAAA GCAGAAGACC AAGGACAATG ATAAAGCCAA GGGGAGGAGC 
CCTGAGGATG ACGAAGAGGA GCGCAGGCGC AGGGAGCGCG AGGACCAGAT GTACCGAGAG 
CGGCTGCGCA CCCTCTTCGT CATCGCAGTG ATCATGAGTC TGCTCAATGC GCTGGGCTCC 
AGTGGAGGCA ACATCTCCTG GAATGACTTC GTGCACGAGA TGCTGGCCAA GGGCGAGGTG 
CAGCGGGTGC AAGTGGTGCC CGAGAGCGAC GTGGTGGAGG TGTACCTGCA CCCCGGTGCC 
GTGGTGTTTG GGCGGCCTCG GCTGGCACTG ATGTACCGAA TGCAGGTGGC AAACATCGAC 
AAATTTGAAG AGAAGCTTCG AGCAGCTGAG GATGAGCTGA ATATCGAAGG CAAGGACAGG 
ATTCCAGTTT CCTACAAGCG GACGGGATTC TTTGGAAATG CCCTCTACGC CCTGGGGATG 
ACGGCCGTGG GCCTGGCCAT CCTGTGGTAT GTTTTCCGTC TGGCTGGGAT GACAGGACGG 
GAAGGCGGAT TCAGTGCTTT TAATCAGCTG AAAATGGCTC GTTTCACGAT TGTGGATGGC 
AAGATGGGGA AAGGAGTCAG CTTCAAAGAT GTGGCAGGAA TGCATGAAGC CAAACTGGAG 
GTCAAGGAGT TCGTGGATTA TCTGAAGAGC CCGGAGCGCT TCCTTCAGCT TGGCGCCAAG 
GTCCCGAAGG GCGCACTGCT GCTCGGCCCC CCCGGCTGCG GGAAGACGCT GCTGGCCAAG 
GCAGTGGCCA CGGAGGCCCA GGTGCCCTTC CTGGCAATGG CTGGCCCAGA GTTCGTGGAG 
GTCATTGGAG GCCTCGGTGC GGCCCGTGTG CGGAGCCTCT TCAAGGAAGC CCGGGCCCGG 
GCCCCCTGCA TCGTGTATAT CGACGAGATC GACGCCGTGG GCAAGAAGCG TTCCACCGCC 
GTGTCCGGCT TCTCCAACAC AGAGGAGGAG CAGACGCTCA ACCAGCTGCT GGTGGAGATG 
GACGGAATGG GTACCACCGA CCACGTCATC GTCCTGGCGG CCACCAACCG AGCTGACATT 
TTGGACAACG CTCTGCTAAG GCCGGGCCGA CTGGACAGGC ACGTTTTCAT CGACCTTCCC 
ACGCTGCAGG AAAGGAAGGA GATCTTTGAG CAGCACCTGA AGAGCCTGAA GCTGACGCGG 
GCCAGCAGCT TTTACTCCCA GCGGCTGGCG GAGCTGACAC CTGGCTTCAG TGGCGCTGAC 
ATTGCCAACA TCTGTAACGA GGCCGCGTTG CACGCGGCGC GGGAGGGGCA CACGGCTGTG 
CACACATCCA ACCTGGACTA CGCCGTGGAG CGTGTCGTGG CCGGGACTGC CAAGAAGAGC 
AAAGTCCTTT CCAAGGAAGA GCAGAAGGTG GTTGCGTTCC ATGAGTCGGG CCACGCCCTG 
GTGGGCTGGC TGCTGGAGCA CACGGAGGCT GTGATGAAGC CTGGGTCCTG CCAGCTAACA 
GACCACCTCA CTGTGCAGGT GTCCATCACT CCTCGGACCA ATGCAGCCCT GGGCTTCGCT 
CAGATGCTCC CGAGAGACCA GCACCTCTTC ACCAGGGAGC AGCTATTCGA GCGCATGTGC 
ATGGCCCTGG GCGGCCGTGC ATCCGAGTCC ATCTCTTTCA ACAGGGTCAC TTCCGGGGCA 
CAGGACGACT TGCGGAAGGT CACCCGCATC GCCTACTCCA TGGTGAAGCA GTTTGGGATG 
GCACCCAGCA TCGGGCCTGT CTCCTTCCCC GAGGCCCAGG AGGGCGTCAC GGGCATCGGA 
CGGCGTCCAT TCAGCCAGGG CCTCCAGCAG ATGATAGACC ACGAAGCGAG GCTGCTGGTG 
GCCACTGCCT ATAGGCACAC GGAACAGGTG CTTCGGGACA ACCTGGACAA GCTCCACGCG 
CTGGCGAGTG CCCTGCTGGA AAAGGAAGTG ATCAACTACG AGGACATCGA GGCCCTCATC 
GGCCCTCCGC CCCACGGGCC CAAGAAAAAG ATCGCGCCGC AGAAGTGGAG CGATGCCCAG 
AGGGAGAAGC AGGACTCGGG GGAGGAGGAG GGAGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SPG7 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム