Spg7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spg7
Entrez Gene ID	31253
Full Name	CG2658 gene product from transcript CG2658-RC
Synonyms	Dm100G10.aj,Dmel\CG2658,EG:100G10.7,anon-3Ba
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3B2-3B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_130661.2	NP_570017.1	paraplegin, isoform A
NM_206616.2	NP_996339.2	paraplegin, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPG7	XP_001140249.3
	SPG7	XP_002802648.1
Rattus norvegicus (Norway rat)	Spg7	NP_852053.1
Danio rerio (zebrafish)	spg7	XP_001923118.1
Gallus gallus (chicken)	SPG7	NP_001012545.1
Drosophila melanogaster (fruit fly)	CG2658	NP_570017.1
Caenorhabditis elegans (roundworm)	ppgn-1	NP_500191.3
Mus musculus (house mouse)	Spg7	NP_694816.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000560	XP_310523.5
Homo sapiens (human)	SPG7	NP_003110.1
Canis lupus familiaris (dog)	SPG7	XP_546777.3
Bos taurus (cattle)	SPG7	NP_001095492.1
Xenopus tropicalis (tropical clawed frog)	spg7	XP_002933705.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spg7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spg7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00632	NM_206616.2 Latest version!	Drosophila melanogaster paraplegin, transcript variant C (Spg7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00632	NM_130661.2 Latest version!	Drosophila melanogaster paraplegin, transcript variant A (Spg7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spg7 ( NM_130661.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_570017.1 Drosophila melanogaster paraplegin, transcript variant A (Spg7), mRNA.

Price & Availability↑

CloneID	OFa00632
Clone ID Related Accession (Same CDS sequence)	NM_130661.2 , NM_206616.2
Accession Version	NM_130661.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paraplegin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_130661.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGAAGG GCCTGCGGCA TGTGCGGAAT GCGCTGCAGC GCGGTGGATC TCAGCTGATC 
CAGCTCCGCA GTGCCACAGG ACAACCCCCA AGGATTGGGC GGGAACTGTC GCTCCTTCAG 
CTCAGCCGGC GGCAGGTGAT GCAGCTGCAG GCGGAGTACA AGGCAATTGT GGGCCTAATA 
GCGCGCTCCC TGCAGCTGAC TCCCAAGGAG GTTAGACTGA TGCACCTGCG CAGCCTGAGC 
AGCACCGCAA AACCTCCTAC CGCTTCGGAT GGCAAGACGG AAAAGCATGA ACAGCCCGAG 
AAGGGCAGTG CCAAGGCTAA AGATGATTCG TCGAAGGAGA AAAAGTCAGC TGTCGCGGCG 
GAGGATGGCG ATAAGAGCAG CAGAGCGGCA GGAGAGGAGG CCGCCAGTAC TCCTACTTCT 
AGCAGTACCA TTGGTGAGCC CGGCGAAGAT CCCAACAAAA ACAGCAACGA GAATGACGAA 
AAGATGCGTT CTGTACTGAC TAAGGCCGTA CTGTGGCTCT TTACCATCTA CATGTTTGTG 
GCCTTCTTTT CCCTGCTAAT TACTCCGCGC TCCGAGAGAC CAGAGGGTTC CACGCGCTAT 
GTGTCGTGGA ACGAGTTCGT GCACCACATG CTGGCCGTGG GCGAAGTCAA GGAGCTGATC 
ATCAGACCCG ACATGGAAAT GGTGACCATC ATTCTGCACG AGGGAGCCGT CATCAAGGGT 
CGGAAGGTGT CTTCCACCAT CTTTCACATG GCGGTGGCCG ATGCCAACAA GTTTGAGGAG 
AAGCTGCGTG ATGTGGAGAA GCGTTTGGGC ATCAAGGATG GTGTGCCGGT GACCTACGAC 
CGGCAGACGG ACACCACCGG ACGTATTCTC ATGCTGCTTC TCGTCTGCGC TCTGCTTATG 
TCGATTGCCA CCCGCATGAA GAGCATCAAG AGCCCGCTCA GCATGGATTC ATTTAACCAA 
ATGGGCCGTG CCAAGTTCAC GCTAGTTGAT CCTTTCGATG GCGGTCGCGG TGTGCTTTTC 
CGGGACGTGG CCGGTCTCAG TGAGGCGAAA CAGGAGGTGA AGGAATTTGT AGATTACCTC 
AAGTCGCCGG AGAAGTACCA GCGGCTGGGC GCCAAGGTGC CGCGAGGAGC CCTACTCCTG 
GGTCCACCAG GATGCGGCAA GACGCTACTG GCCAAGGCGG TCGCCACCGA GGCCCAGGTG 
CCGTTTCTCA GCATGAACGG CTCCGAGTTC ATCGAGATGA TTGGCGGTCT GGGGGCCGCG 
CGAGTGCGTG ATCTGTTTAA GGAAGGCAAA AAACGGGCGC CATGCATCAT TTACATCGAT 
GAGATAGACG CCATCGGTCG GCAACGTTCG GGAACTGAAA GCATGGGACA GGGCAGCTCT 
GGCGAATCGG AGCAGACGCT TAATCAGCTG CTGGTCGAGA TGGACGGTAT GGCCACCAAG 
GAGGGCGTGC TGATGTTGGC CAGTACGAAT CGAGCTGATA TCCTAGACAA GGCGCTGCTG 
CGACCCGGTC GCTTTGATCG CCACATTCTC ATCGATTTGC CCACGCTGGC GGAGCGCAAG 
GAGATCTTTG AGAAGCACTT GTCATCGGTG AAACTGGAGT CGCCGCCCAC GACCTTCTCA 
CAACGCTTGG CCCGCCTGAC ACCGGGTTTT TCGGGTGCTG ACATCGCCAA CGTATGCAAT 
GAGGCCGCCC TGCATGCGGC GCGCAACACA CAGATGGAGG TCAGCAGCAA GAATCTTGAG 
TACGCCGTTG AGCGTCTAGT TGGCGGAACT GAGAAACGTT CGCATGCCCT ATCGCTGGCG 
GAGCGCAAGG TGATCGCTTA CCACGAATCG GGACACGCGC TGGTGGGCTG GATGTTGCCC 
AACTCGGACA TCCTGCTTAA GGTGACCATT GTGCCGCGCA CAAGTCTGGC GCTCGGCTTC 
GCCCAATATA CGCCCAGCGA ACAGCATTTG TACAGCAAGG AGGAACTGTT CGACAAGATG 
TGCATGGCGC TCGGCGGGCG CGCTGCCGAG AATCTAGTCT TTAACCGCAT AACGACGGGA 
GCTCAAAACG ATCTGGAAAA GGTGACGAAG ATCGCGTACT CGCAGATCAA GAAGTTCGGC 
ATGAACGACA CCCTCGGTCC CATCTACGTA CGCGACGCGG ATGAGACCGA GGGCGGCGGT 
GCGATGGGCA GCGGTGGCAA AAAGCCCTTC TCGCGTGCCA TGGAGAGCAT GATTGACAAC 
GAGGCGCGGC ATGTGGTGGC GAGTGCTTAC CAAACCACCG AGGGCATACT CACTACGCAT 
CGCGATAAGC TGGAGAAGCT GGCCGAAGCT CTGCTGGAGA AGGAAACGCT GGACTACGAC 
CAGGTGGTGC AGCTAATCGG ACCCCCGCCA TACGATCTGG GCAAGCGGCA GGTAGAGAGC 
GTGGAGTTTG AGCAGTCACT GAAGAACCTA AGCACCGACA CGGATGCCAC AAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570017.1
CDS	229..2688
Translation	MLKGLRHVRNALQRGGSQLIQLRSATGQPPRIGRELSLLQLSRRQVMQLQAEYKAIVGLIARSLQLTPKEVRLMHLRSLSSTAKPPTASDGKTEKHEQPEKGSAKAKDDSSKEKKSAVAAEDGDKSSRAAGEEAASTPTSSSTIGEPGEDPNKNSNENDEKMRSVLTKAVLWLFTIYMFVAFFSLLITPRSERPEGSTRYVSWNEFVHHMLAVGEVKELIIRPDMEMVTIILHEGAVIKGRKVSSTIFHMAVADANKFEEKLRDVEKRLGIKDGVPVTYDRQTDTTGRILMLLLVCALLMSIATRMKSIKSPLSMDSFNQMGRAKFTLVDPFDGGRGVLFRDVAGLSEAKQEVKEFVDYLKSPEKYQRLGAKVPRGALLLGPPGCGKTLLAKAVATEAQVPFLSMNGSEFIEMIGGLGAARVRDLFKEGKKRAPCIIYIDEIDAIGRQRSGTESMGQGSSGESEQTLNQLLVEMDGMATKEGVLMLASTNRADILDKALLRPGRFDRHILIDLPTLAERKEIFEKHLSSVKLESPPTTFSQRLARLTPGFSGADIANVCNEAALHAARNTQMEVSSKNLEYAVERLVGGTEKRSHALSLAERKVIAYHESGHALVGWMLPNSDILLKVTIVPRTSLALGFAQYTPSEQHLYSKEELFDKMCMALGGRAAENLVFNRITTGAQNDLEKVTKIAYSQIKKFGMNDTLGPIYVRDADETEGGGAMGSGGKKPFSRAMESMIDNEARHVVASAYQTTEGILTTHRDKLEKLAEALLEKETLDYDQVVQLIGPPPYDLGKRQVESVEFEQSLKNLSTDTDATKA

Target ORF information:

RefSeq Version	NM_130661.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paraplegin, transcript variant A (Spg7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130661.2

ORF Insert Sequence:

ATGTTGAAGG GCCTGCGGCA TGTGCGGAAT GCGCTGCAGC GCGGTGGATC TCAGCTGATC 
CAGCTCCGCA GTGCCACAGG ACAACCCCCA AGGATTGGGC GGGAACTGTC GCTCCTTCAG 
CTCAGCCGGC GGCAGGTGAT GCAGCTGCAG GCGGAGTACA AGGCAATTGT GGGCCTAATA 
GCGCGCTCCC TGCAGCTGAC TCCCAAGGAG GTTAGACTGA TGCACCTGCG CAGCCTGAGC 
AGCACCGCAA AACCTCCTAC CGCTTCGGAT GGCAAGACGG AAAAGCATGA ACAGCCCGAG 
AAGGGCAGTG CCAAGGCTAA AGATGATTCG TCGAAGGAGA AAAAGTCAGC TGTCGCGGCG 
GAGGATGGCG ATAAGAGCAG CAGAGCGGCA GGAGAGGAGG CCGCCAGTAC TCCTACTTCT 
AGCAGTACCA TTGGTGAGCC CGGCGAAGAT CCCAACAAAA ACAGCAACGA GAATGACGAA 
AAGATGCGTT CTGTACTGAC TAAGGCCGTA CTGTGGCTCT TTACCATCTA CATGTTTGTG 
GCCTTCTTTT CCCTGCTAAT TACTCCGCGC TCCGAGAGAC CAGAGGGTTC CACGCGCTAT 
GTGTCGTGGA ACGAGTTCGT GCACCACATG CTGGCCGTGG GCGAAGTCAA GGAGCTGATC 
ATCAGACCCG ACATGGAAAT GGTGACCATC ATTCTGCACG AGGGAGCCGT CATCAAGGGT 
CGGAAGGTGT CTTCCACCAT CTTTCACATG GCGGTGGCCG ATGCCAACAA GTTTGAGGAG 
AAGCTGCGTG ATGTGGAGAA GCGTTTGGGC ATCAAGGATG GTGTGCCGGT GACCTACGAC 
CGGCAGACGG ACACCACCGG ACGTATTCTC ATGCTGCTTC TCGTCTGCGC TCTGCTTATG 
TCGATTGCCA CCCGCATGAA GAGCATCAAG AGCCCGCTCA GCATGGATTC ATTTAACCAA 
ATGGGCCGTG CCAAGTTCAC GCTAGTTGAT CCTTTCGATG GCGGTCGCGG TGTGCTTTTC 
CGGGACGTGG CCGGTCTCAG TGAGGCGAAA CAGGAGGTGA AGGAATTTGT AGATTACCTC 
AAGTCGCCGG AGAAGTACCA GCGGCTGGGC GCCAAGGTGC CGCGAGGAGC CCTACTCCTG 
GGTCCACCAG GATGCGGCAA GACGCTACTG GCCAAGGCGG TCGCCACCGA GGCCCAGGTG 
CCGTTTCTCA GCATGAACGG CTCCGAGTTC ATCGAGATGA TTGGCGGTCT GGGGGCCGCG 
CGAGTGCGTG ATCTGTTTAA GGAAGGCAAA AAACGGGCGC CATGCATCAT TTACATCGAT 
GAGATAGACG CCATCGGTCG GCAACGTTCG GGAACTGAAA GCATGGGACA GGGCAGCTCT 
GGCGAATCGG AGCAGACGCT TAATCAGCTG CTGGTCGAGA TGGACGGTAT GGCCACCAAG 
GAGGGCGTGC TGATGTTGGC CAGTACGAAT CGAGCTGATA TCCTAGACAA GGCGCTGCTG 
CGACCCGGTC GCTTTGATCG CCACATTCTC ATCGATTTGC CCACGCTGGC GGAGCGCAAG 
GAGATCTTTG AGAAGCACTT GTCATCGGTG AAACTGGAGT CGCCGCCCAC GACCTTCTCA 
CAACGCTTGG CCCGCCTGAC ACCGGGTTTT TCGGGTGCTG ACATCGCCAA CGTATGCAAT 
GAGGCCGCCC TGCATGCGGC GCGCAACACA CAGATGGAGG TCAGCAGCAA GAATCTTGAG 
TACGCCGTTG AGCGTCTAGT TGGCGGAACT GAGAAACGTT CGCATGCCCT ATCGCTGGCG 
GAGCGCAAGG TGATCGCTTA CCACGAATCG GGACACGCGC TGGTGGGCTG GATGTTGCCC 
AACTCGGACA TCCTGCTTAA GGTGACCATT GTGCCGCGCA CAAGTCTGGC GCTCGGCTTC 
GCCCAATATA CGCCCAGCGA ACAGCATTTG TACAGCAAGG AGGAACTGTT CGACAAGATG 
TGCATGGCGC TCGGCGGGCG CGCTGCCGAG AATCTAGTCT TTAACCGCAT AACGACGGGA 
GCTCAAAACG ATCTGGAAAA GGTGACGAAG ATCGCGTACT CGCAGATCAA GAAGTTCGGC 
ATGAACGACA CCCTCGGTCC CATCTACGTA CGCGACGCGG ATGAGACCGA GGGCGGCGGT 
GCGATGGGCA GCGGTGGCAA AAAGCCCTTC TCGCGTGCCA TGGAGAGCAT GATTGACAAC 
GAGGCGCGGC ATGTGGTGGC GAGTGCTTAC CAAACCACCG AGGGCATACT CACTACGCAT 
CGCGATAAGC TGGAGAAGCT GGCCGAAGCT CTGCTGGAGA AGGAAACGCT GGACTACGAC 
CAGGTGGTGC AGCTAATCGG ACCCCCGCCA TACGATCTGG GCAAGCGGCA GGTAGAGAGC 
GTGGAGTTTG AGCAGTCACT GAAGAACCTA AGCACCGACA CGGATGCCAC AAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg7 ( NM_206616.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996339.2 Drosophila melanogaster paraplegin, transcript variant C (Spg7), mRNA.

Price & Availability↑

CloneID	OFa00632
Clone ID Related Accession (Same CDS sequence)	NM_130661.2 , NM_206616.2
Accession Version	NM_206616.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	paraplegin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206616.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGAAGG GCCTGCGGCA TGTGCGGAAT GCGCTGCAGC GCGGTGGATC TCAGCTGATC 
CAGCTCCGCA GTGCCACAGG ACAACCCCCA AGGATTGGGC GGGAACTGTC GCTCCTTCAG 
CTCAGCCGGC GGCAGGTGAT GCAGCTGCAG GCGGAGTACA AGGCAATTGT GGGCCTAATA 
GCGCGCTCCC TGCAGCTGAC TCCCAAGGAG GTTAGACTGA TGCACCTGCG CAGCCTGAGC 
AGCACCGCAA AACCTCCTAC CGCTTCGGAT GGCAAGACGG AAAAGCATGA ACAGCCCGAG 
AAGGGCAGTG CCAAGGCTAA AGATGATTCG TCGAAGGAGA AAAAGTCAGC TGTCGCGGCG 
GAGGATGGCG ATAAGAGCAG CAGAGCGGCA GGAGAGGAGG CCGCCAGTAC TCCTACTTCT 
AGCAGTACCA TTGGTGAGCC CGGCGAAGAT CCCAACAAAA ACAGCAACGA GAATGACGAA 
AAGATGCGTT CTGTACTGAC TAAGGCCGTA CTGTGGCTCT TTACCATCTA CATGTTTGTG 
GCCTTCTTTT CCCTGCTAAT TACTCCGCGC TCCGAGAGAC CAGAGGGTTC CACGCGCTAT 
GTGTCGTGGA ACGAGTTCGT GCACCACATG CTGGCCGTGG GCGAAGTCAA GGAGCTGATC 
ATCAGACCCG ACATGGAAAT GGTGACCATC ATTCTGCACG AGGGAGCCGT CATCAAGGGT 
CGGAAGGTGT CTTCCACCAT CTTTCACATG GCGGTGGCCG ATGCCAACAA GTTTGAGGAG 
AAGCTGCGTG ATGTGGAGAA GCGTTTGGGC ATCAAGGATG GTGTGCCGGT GACCTACGAC 
CGGCAGACGG ACACCACCGG ACGTATTCTC ATGCTGCTTC TCGTCTGCGC TCTGCTTATG 
TCGATTGCCA CCCGCATGAA GAGCATCAAG AGCCCGCTCA GCATGGATTC ATTTAACCAA 
ATGGGCCGTG CCAAGTTCAC GCTAGTTGAT CCTTTCGATG GCGGTCGCGG TGTGCTTTTC 
CGGGACGTGG CCGGTCTCAG TGAGGCGAAA CAGGAGGTGA AGGAATTTGT AGATTACCTC 
AAGTCGCCGG AGAAGTACCA GCGGCTGGGC GCCAAGGTGC CGCGAGGAGC CCTACTCCTG 
GGTCCACCAG GATGCGGCAA GACGCTACTG GCCAAGGCGG TCGCCACCGA GGCCCAGGTG 
CCGTTTCTCA GCATGAACGG CTCCGAGTTC ATCGAGATGA TTGGCGGTCT GGGGGCCGCG 
CGAGTGCGTG ATCTGTTTAA GGAAGGCAAA AAACGGGCGC CATGCATCAT TTACATCGAT 
GAGATAGACG CCATCGGTCG GCAACGTTCG GGAACTGAAA GCATGGGACA GGGCAGCTCT 
GGCGAATCGG AGCAGACGCT TAATCAGCTG CTGGTCGAGA TGGACGGTAT GGCCACCAAG 
GAGGGCGTGC TGATGTTGGC CAGTACGAAT CGAGCTGATA TCCTAGACAA GGCGCTGCTG 
CGACCCGGTC GCTTTGATCG CCACATTCTC ATCGATTTGC CCACGCTGGC GGAGCGCAAG 
GAGATCTTTG AGAAGCACTT GTCATCGGTG AAACTGGAGT CGCCGCCCAC GACCTTCTCA 
CAACGCTTGG CCCGCCTGAC ACCGGGTTTT TCGGGTGCTG ACATCGCCAA CGTATGCAAT 
GAGGCCGCCC TGCATGCGGC GCGCAACACA CAGATGGAGG TCAGCAGCAA GAATCTTGAG 
TACGCCGTTG AGCGTCTAGT TGGCGGAACT GAGAAACGTT CGCATGCCCT ATCGCTGGCG 
GAGCGCAAGG TGATCGCTTA CCACGAATCG GGACACGCGC TGGTGGGCTG GATGTTGCCC 
AACTCGGACA TCCTGCTTAA GGTGACCATT GTGCCGCGCA CAAGTCTGGC GCTCGGCTTC 
GCCCAATATA CGCCCAGCGA ACAGCATTTG TACAGCAAGG AGGAACTGTT CGACAAGATG 
TGCATGGCGC TCGGCGGGCG CGCTGCCGAG AATCTAGTCT TTAACCGCAT AACGACGGGA 
GCTCAAAACG ATCTGGAAAA GGTGACGAAG ATCGCGTACT CGCAGATCAA GAAGTTCGGC 
ATGAACGACA CCCTCGGTCC CATCTACGTA CGCGACGCGG ATGAGACCGA GGGCGGCGGT 
GCGATGGGCA GCGGTGGCAA AAAGCCCTTC TCGCGTGCCA TGGAGAGCAT GATTGACAAC 
GAGGCGCGGC ATGTGGTGGC GAGTGCTTAC CAAACCACCG AGGGCATACT CACTACGCAT 
CGCGATAAGC TGGAGAAGCT GGCCGAAGCT CTGCTGGAGA AGGAAACGCT GGACTACGAC 
CAGGTGGTGC AGCTAATCGG ACCCCCGCCA TACGATCTGG GCAAGCGGCA GGTAGAGAGC 
GTGGAGTTTG AGCAGTCACT GAAGAACCTA AGCACCGACA CGGATGCCAC AAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996339.2
CDS	229..2688
Translation	MLKGLRHVRNALQRGGSQLIQLRSATGQPPRIGRELSLLQLSRRQVMQLQAEYKAIVGLIARSLQLTPKEVRLMHLRSLSSTAKPPTASDGKTEKHEQPEKGSAKAKDDSSKEKKSAVAAEDGDKSSRAAGEEAASTPTSSSTIGEPGEDPNKNSNENDEKMRSVLTKAVLWLFTIYMFVAFFSLLITPRSERPEGSTRYVSWNEFVHHMLAVGEVKELIIRPDMEMVTIILHEGAVIKGRKVSSTIFHMAVADANKFEEKLRDVEKRLGIKDGVPVTYDRQTDTTGRILMLLLVCALLMSIATRMKSIKSPLSMDSFNQMGRAKFTLVDPFDGGRGVLFRDVAGLSEAKQEVKEFVDYLKSPEKYQRLGAKVPRGALLLGPPGCGKTLLAKAVATEAQVPFLSMNGSEFIEMIGGLGAARVRDLFKEGKKRAPCIIYIDEIDAIGRQRSGTESMGQGSSGESEQTLNQLLVEMDGMATKEGVLMLASTNRADILDKALLRPGRFDRHILIDLPTLAERKEIFEKHLSSVKLESPPTTFSQRLARLTPGFSGADIANVCNEAALHAARNTQMEVSSKNLEYAVERLVGGTEKRSHALSLAERKVIAYHESGHALVGWMLPNSDILLKVTIVPRTSLALGFAQYTPSEQHLYSKEELFDKMCMALGGRAAENLVFNRITTGAQNDLEKVTKIAYSQIKKFGMNDTLGPIYVRDADETEGGGAMGSGGKKPFSRAMESMIDNEARHVVASAYQTTEGILTTHRDKLEKLAEALLEKETLDYDQVVQLIGPPPYDLGKRQVESVEFEQSLKNLSTDTDATKA

Target ORF information:

RefSeq Version	NM_206616.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster paraplegin, transcript variant C (Spg7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206616.2

ORF Insert Sequence:

ATGTTGAAGG GCCTGCGGCA TGTGCGGAAT GCGCTGCAGC GCGGTGGATC TCAGCTGATC 
CAGCTCCGCA GTGCCACAGG ACAACCCCCA AGGATTGGGC GGGAACTGTC GCTCCTTCAG 
CTCAGCCGGC GGCAGGTGAT GCAGCTGCAG GCGGAGTACA AGGCAATTGT GGGCCTAATA 
GCGCGCTCCC TGCAGCTGAC TCCCAAGGAG GTTAGACTGA TGCACCTGCG CAGCCTGAGC 
AGCACCGCAA AACCTCCTAC CGCTTCGGAT GGCAAGACGG AAAAGCATGA ACAGCCCGAG 
AAGGGCAGTG CCAAGGCTAA AGATGATTCG TCGAAGGAGA AAAAGTCAGC TGTCGCGGCG 
GAGGATGGCG ATAAGAGCAG CAGAGCGGCA GGAGAGGAGG CCGCCAGTAC TCCTACTTCT 
AGCAGTACCA TTGGTGAGCC CGGCGAAGAT CCCAACAAAA ACAGCAACGA GAATGACGAA 
AAGATGCGTT CTGTACTGAC TAAGGCCGTA CTGTGGCTCT TTACCATCTA CATGTTTGTG 
GCCTTCTTTT CCCTGCTAAT TACTCCGCGC TCCGAGAGAC CAGAGGGTTC CACGCGCTAT 
GTGTCGTGGA ACGAGTTCGT GCACCACATG CTGGCCGTGG GCGAAGTCAA GGAGCTGATC 
ATCAGACCCG ACATGGAAAT GGTGACCATC ATTCTGCACG AGGGAGCCGT CATCAAGGGT 
CGGAAGGTGT CTTCCACCAT CTTTCACATG GCGGTGGCCG ATGCCAACAA GTTTGAGGAG 
AAGCTGCGTG ATGTGGAGAA GCGTTTGGGC ATCAAGGATG GTGTGCCGGT GACCTACGAC 
CGGCAGACGG ACACCACCGG ACGTATTCTC ATGCTGCTTC TCGTCTGCGC TCTGCTTATG 
TCGATTGCCA CCCGCATGAA GAGCATCAAG AGCCCGCTCA GCATGGATTC ATTTAACCAA 
ATGGGCCGTG CCAAGTTCAC GCTAGTTGAT CCTTTCGATG GCGGTCGCGG TGTGCTTTTC 
CGGGACGTGG CCGGTCTCAG TGAGGCGAAA CAGGAGGTGA AGGAATTTGT AGATTACCTC 
AAGTCGCCGG AGAAGTACCA GCGGCTGGGC GCCAAGGTGC CGCGAGGAGC CCTACTCCTG 
GGTCCACCAG GATGCGGCAA GACGCTACTG GCCAAGGCGG TCGCCACCGA GGCCCAGGTG 
CCGTTTCTCA GCATGAACGG CTCCGAGTTC ATCGAGATGA TTGGCGGTCT GGGGGCCGCG 
CGAGTGCGTG ATCTGTTTAA GGAAGGCAAA AAACGGGCGC CATGCATCAT TTACATCGAT 
GAGATAGACG CCATCGGTCG GCAACGTTCG GGAACTGAAA GCATGGGACA GGGCAGCTCT 
GGCGAATCGG AGCAGACGCT TAATCAGCTG CTGGTCGAGA TGGACGGTAT GGCCACCAAG 
GAGGGCGTGC TGATGTTGGC CAGTACGAAT CGAGCTGATA TCCTAGACAA GGCGCTGCTG 
CGACCCGGTC GCTTTGATCG CCACATTCTC ATCGATTTGC CCACGCTGGC GGAGCGCAAG 
GAGATCTTTG AGAAGCACTT GTCATCGGTG AAACTGGAGT CGCCGCCCAC GACCTTCTCA 
CAACGCTTGG CCCGCCTGAC ACCGGGTTTT TCGGGTGCTG ACATCGCCAA CGTATGCAAT 
GAGGCCGCCC TGCATGCGGC GCGCAACACA CAGATGGAGG TCAGCAGCAA GAATCTTGAG 
TACGCCGTTG AGCGTCTAGT TGGCGGAACT GAGAAACGTT CGCATGCCCT ATCGCTGGCG 
GAGCGCAAGG TGATCGCTTA CCACGAATCG GGACACGCGC TGGTGGGCTG GATGTTGCCC 
AACTCGGACA TCCTGCTTAA GGTGACCATT GTGCCGCGCA CAAGTCTGGC GCTCGGCTTC 
GCCCAATATA CGCCCAGCGA ACAGCATTTG TACAGCAAGG AGGAACTGTT CGACAAGATG 
TGCATGGCGC TCGGCGGGCG CGCTGCCGAG AATCTAGTCT TTAACCGCAT AACGACGGGA 
GCTCAAAACG ATCTGGAAAA GGTGACGAAG ATCGCGTACT CGCAGATCAA GAAGTTCGGC 
ATGAACGACA CCCTCGGTCC CATCTACGTA CGCGACGCGG ATGAGACCGA GGGCGGCGGT 
GCGATGGGCA GCGGTGGCAA AAAGCCCTTC TCGCGTGCCA TGGAGAGCAT GATTGACAAC 
GAGGCGCGGC ATGTGGTGGC GAGTGCTTAC CAAACCACCG AGGGCATACT CACTACGCAT 
CGCGATAAGC TGGAGAAGCT GGCCGAAGCT CTGCTGGAGA AGGAAACGCT GGACTACGAC 
CAGGTGGTGC AGCTAATCGG ACCCCCGCCA TACGATCTGG GCAAGCGGCA GGTAGAGAGC 
GTGGAGTTTG AGCAGTCACT GAAGAACCTA AGCACCGACA CGGATGCCAC AAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Spg7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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