FAP cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	FAP
Entrez Gene ID	508882
Full Name	fibroblast activation protein alpha
Synonyms	SIMP,ZIP
General protein information	Preferred Names fibroblast activation protein alpha Names prolyl endopeptidase FAP FAPalpha Z-Pro-prolinal insensitive peptidase dipeptidyl peptidase FAP fibroblast activation protein, alpha subunit gelatine degradation protease FAP integral membrane serine protease post-proline cleaving enzyme seprase serine integral membrane protease surface-expressed protease
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010801968.2	XP_010800270.1	prolyl endopeptidase FAP isoform X1
NM_001098001.1	NP_001091470.1	prolyl endopeptidase FAP
XM_010801969.2	XP_010800271.1	prolyl endopeptidase FAP isoform X2
XM_010801968.3	XP_010800270.1	prolyl endopeptidase FAP isoform X1
XM_010801969.3	XP_010800271.1	prolyl endopeptidase FAP isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Fap	NP_032012.1
Gallus gallus (chicken)	FAP	NP_001180568.1
Pan troglodytes (chimpanzee)	FAP	XP_515860.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011584	XP_309924.4
Xenopus tropicalis (tropical clawed frog)	fap	NP_001107145.1
Drosophila melanogaster (fruit fly)	ome	NP_001097606.1
	FAP	XP_001096858.1
Bos taurus (cattle)	FAP	NP_001091470.1
Rattus norvegicus (Norway rat)	Fap	NP_620205.1
Magnaporthe oryzae (rice blast fungus)	MGG_05989	XP_003711833.1
Homo sapiens (human)	FAP	NP_004451.2
Canis lupus familiaris (dog)	FAP	XP_005640309.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Fibroblast activation protein alpha is expressed by chondrocytes following a pro-inflammatory stimulus and is elevated in osteoarthritis.
Milner JM, Kevorkian L, Young DA, Jones D, Wait R, Donell ST, Barksby E, Patterson AM, Middleton J, Cravatt BF, Clark IM, Rowan AD, Cawston TE
Arthritis research & therapy8(1)R23(2006)

Purification, identification and characterisation of seprase from bovine serum.
Collins PJ, McMahon G, O'Brien P, O'Connor B
The international journal of biochemistry & cell biology36(11)2320-33(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We clearly show an association between FAPalpha and chondrocytes in the context of cartilage degradation. (Fibroblast activation protein alpha)
Title: Fibroblast activation protein alpha is expressed by chondrocytes following a pro-inflammatory stimulus and is elevated in osteoarthritis.

Soluble form of a seprase activity is detected in bovine serum
Title: Purification, identification and characterisation of seprase from bovine serum.

The following FAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa47279	XM_010801969.3 Latest version!	Bos taurus fibroblast activation protein alpha (FAP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa02333	NM_001098001.1 Latest version!	Bos taurus fibroblast activation protein alpha (FAP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa47278	XM_010801968.3 Latest version!	Bos taurus fibroblast activation protein alpha (FAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa47278	XM_010801968.2	Bos taurus fibroblast activation protein alpha (FAP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa47279	XM_010801969.2	Bos taurus fibroblast activation protein alpha (FAP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FAP ( XM_010801969.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800271.1 Bos taurus fibroblast activation protein alpha (FAP), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa47279
Clone ID Related Accession (Same CDS sequence)	XM_010801969.2 , XM_010801969.3
Accession Version	XM_010801969.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	prolyl endopeptidase FAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAT GGTTAAAAAT TGTATTTGGA GTTGCCACCT CTGCCGTGCT TGCTTTACTG 
GTGATGTGTA TTGTCTTACG TCCTTCAAGA GTTCACAACT CTGAAGAGAG TACAACACGA 
GCACTCACAC TGAAGGATAT TTTAAATGGG ACATTTTCTT ATAAAACATT TTTTCCAAAT 
TGGATTTCAG GACAAGAATA TCTTCATCAA TCTACAGATA ATAATGTAGT ATTTTATAAT 
ATTGAAACAG GAGAATCATA TACCATTTTG AGTAATACCA CCATGAAAAG TGTGAATGCT 
TCAAATTATG GTTTATCACC TGATCGGCAA TTTGCATACC TAGAAAGTGA TTATTCAAAG 
CTTTGGAGAT ACTCTTACAC AGCAACATAT CACATCTACG ACCTTACAAA CGGGGAGTTT 
ATAAGAAGAA ATGAGCTTCC TCGTCCAATT CAGTATTTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATC TATCTGAAAC AAAGACCAGA AGACCCACCT 
TTTCAAATAA CATATAATGG AAAAGAAAAT AAAATATTTA ATGGAATCCC AGACTGGGTT 
TACGAAGAGG AAATGCTTGC TACAAAATAT GCTCTCTGGT GGTCTCCTAA TGGAAAATTT 
TTGGCGTATG CAGAATTTAA TGACACAGAG ATACCAGTTA TTGCCTATTC CTATTATGGT 
GATGAACAAT ATCCTAGAAC AATAAATATT CCATACCCAA AGGCTGGAGC TAAGAATCCT 
GTTGTTCGGA TCTTTATCAT TGATGCCACT TACCCTGAGC ATATAGGTCC CAGAGAAGTG 
CCAGTACCAG CAATGATAGC CTCAAGTGAT TATTATTTCA GTTGGCTCAC ATGGGTTACT 
GATGATCGAA TATGTTTGCA GTGGCTAAAA AGAATCCAAA ATGTTTCAGT GTTGTCTACA 
TGTGATTTCA GGGAAGACTG GCAGACATGG AACTGTCCAA AGACCCAGGA GCATATAGAA 
GAAAGCAGAA CTGGATGGGC TGGTGGATTC TTTGTTTCAA CACCAGTGTT CAGCCATGAT 
ACCATTTCAT ACTACAAAAT ATTTAGTGAC AAGGATGGCT ACAAACATAT TCACTATATC 
AGAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATAAATATA 
TTCAGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG CTACCCTGGA 
AGAAGAAATA TCTATAGAAT TAGCATTGGA AGCCATTCTC CAAGCAAGAA GTGCATTACT 
TGCCATCTAA GGAAAAAAAG GTGCCAATAT TACACAGCAA GTTTCAGCGA CTATGCCAAG 
TACTACGCAC TCGTCTGCTA TGGCCCAGGC CTCCCCATTT CCACCCTTCA TGATGGCCGC 
ACCGACCAAG AAATTAAAAT CCTGGAAGAC AACAAGGAAT TGGAAAATGC TTTGAAAAAT 
ATTCAGCTGC CCAAAGAAGA AATTAAGAAA CTTAAAGTGG ATGATATTAC ATTGTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAAA TCAAAGAAGT ATCCCTTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGTCA GAGTGTAAGG TCTATATTCG CTGTTAGTTG GATATCTTAT 
CTTGCAAGTA AGGAAGGGAT AGTCATTGCC TTGGTGGATG GTCGAGGAAC AGCTTTCCAA 
GGTGACAAAC TCCTGTATGC AGTGTATCGA AAGCTGGGTG TTTATGAAGT TGAGGACCAG 
ATCACAGCTG TCAGTCCTAT GGAGGTTATG TTTCATCGCT GGCCCTTGCT TCAGGAACTG 
GTCTTTTCAA ATGTGGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800271.1
CDS	414..2294
Translation	MKTWLKIVFGVATSAVLALLVMCIVLRPSRVHNSEESTTRALTLKDILNGTFSYKTFFPNWISGQEYLHQSTDNNVVFYNIETGESYTILSNTTMKSVNASNYGLSPDRQFAYLESDYSKLWRYSYTATYHIYDLTNGEFIRRNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPEDPPFQITYNGKENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTEIPVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDATYPEHIGPREVPVPAMIASSDYYFSWLTWVTDDRICLQWLKRIQNVSVLSTCDFREDWQTWNCPKTQEHIEESRTGWAGGFFVSTPVFSHDTISYYKIFSDKDGYKHIHYIRDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGSHSPSKKCITCHLRKKRCQYYTASFSDYAKYYALVCYGPGLPISTLHDGRTDQEIKILEDNKELENALKNIQLPKEEIKKLKVDDITLWYKMILPPQFDKSKKYPLLIQVYGGPCSQSVRSIFAVSWISYLASKEGIVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVSPMEVMFHRWPLLQELVFSNVG

Target ORF information:

RefSeq Version	XM_010801969.2
Organism	Bos taurus(cattle)
Definition	Bos taurus fibroblast activation protein alpha (FAP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801969.2

ORF Insert Sequence:

ATGAAGACAT GGTTAAAAAT TGTATTTGGA GTTGCCACCT CTGCCGTGCT TGCTTTACTG 
GTGATGTGTA TTGTCTTACG TCCTTCAAGA GTTCACAACT CTGAAGAGAG TACAACACGA 
GCACTCACAC TGAAGGATAT TTTAAATGGG ACATTTTCTT ATAAAACATT TTTTCCAAAT 
TGGATTTCAG GACAAGAATA TCTTCATCAA TCTACAGATA ATAATGTAGT ATTTTATAAT 
ATTGAAACAG GAGAATCATA TACCATTTTG AGTAATACCA CCATGAAAAG TGTGAATGCT 
TCAAATTATG GTTTATCACC TGATCGGCAA TTTGCATACC TAGAAAGTGA TTATTCAAAG 
CTTTGGAGAT ACTCTTACAC AGCAACATAT CACATCTACG ACCTTACAAA CGGGGAGTTT 
ATAAGAAGAA ATGAGCTTCC TCGTCCAATT CAGTATTTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATC TATCTGAAAC AAAGACCAGA AGACCCACCT 
TTTCAAATAA CATATAATGG AAAAGAAAAT AAAATATTTA ATGGAATCCC AGACTGGGTT 
TACGAAGAGG AAATGCTTGC TACAAAATAT GCTCTCTGGT GGTCTCCTAA TGGAAAATTT 
TTGGCGTATG CAGAATTTAA TGACACAGAG ATACCAGTTA TTGCCTATTC CTATTATGGT 
GATGAACAAT ATCCTAGAAC AATAAATATT CCATACCCAA AGGCTGGAGC TAAGAATCCT 
GTTGTTCGGA TCTTTATCAT TGATGCCACT TACCCTGAGC ATATAGGTCC CAGAGAAGTG 
CCAGTACCAG CAATGATAGC CTCAAGTGAT TATTATTTCA GTTGGCTCAC ATGGGTTACT 
GATGATCGAA TATGTTTGCA GTGGCTAAAA AGAATCCAAA ATGTTTCAGT GTTGTCTACA 
TGTGATTTCA GGGAAGACTG GCAGACATGG AACTGTCCAA AGACCCAGGA GCATATAGAA 
GAAAGCAGAA CTGGATGGGC TGGTGGATTC TTTGTTTCAA CACCAGTGTT CAGCCATGAT 
ACCATTTCAT ACTACAAAAT ATTTAGTGAC AAGGATGGCT ACAAACATAT TCACTATATC 
AGAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATAAATATA 
TTCAGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG CTACCCTGGA 
AGAAGAAATA TCTATAGAAT TAGCATTGGA AGCCATTCTC CAAGCAAGAA GTGCATTACT 
TGCCATCTAA GGAAAAAAAG GTGCCAATAT TACACAGCAA GTTTCAGCGA CTATGCCAAG 
TACTACGCAC TCGTCTGCTA TGGCCCAGGC CTCCCCATTT CCACCCTTCA TGATGGCCGC 
ACCGACCAAG AAATTAAAAT CCTGGAAGAC AACAAGGAAT TGGAAAATGC TTTGAAAAAT 
ATTCAGCTGC CCAAAGAAGA AATTAAGAAA CTTAAAGTGG ATGATATTAC ATTGTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAAA TCAAAGAAGT ATCCCTTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGTCA GAGTGTAAGG TCTATATTCG CTGTTAGTTG GATATCTTAT 
CTTGCAAGTA AGGAAGGGAT AGTCATTGCC TTGGTGGATG GTCGAGGAAC AGCTTTCCAA 
GGTGACAAAC TCCTGTATGC AGTGTATCGA AAGCTGGGTG TTTATGAAGT TGAGGACCAG 
ATCACAGCTG TCAGTCCTAT GGAGGTTATG TTTCATCGCT GGCCCTTGCT TCAGGAACTG 
GTCTTTTCAA ATGTGGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAP ( XM_010801969.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800271.1 Bos taurus fibroblast activation protein alpha (FAP), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa47279
Clone ID Related Accession (Same CDS sequence)	XM_010801969.2 , XM_010801969.3
Accession Version	XM_010801969.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	prolyl endopeptidase FAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801969.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAT GGTTAAAAAT TGTATTTGGA GTTGCCACCT CTGCCGTGCT TGCTTTACTG 
GTGATGTGTA TTGTCTTACG TCCTTCAAGA GTTCACAACT CTGAAGAGAG TACAACACGA 
GCACTCACAC TGAAGGATAT TTTAAATGGG ACATTTTCTT ATAAAACATT TTTTCCAAAT 
TGGATTTCAG GACAAGAATA TCTTCATCAA TCTACAGATA ATAATGTAGT ATTTTATAAT 
ATTGAAACAG GAGAATCATA TACCATTTTG AGTAATACCA CCATGAAAAG TGTGAATGCT 
TCAAATTATG GTTTATCACC TGATCGGCAA TTTGCATACC TAGAAAGTGA TTATTCAAAG 
CTTTGGAGAT ACTCTTACAC AGCAACATAT CACATCTACG ACCTTACAAA CGGGGAGTTT 
ATAAGAAGAA ATGAGCTTCC TCGTCCAATT CAGTATTTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATC TATCTGAAAC AAAGACCAGA AGACCCACCT 
TTTCAAATAA CATATAATGG AAAAGAAAAT AAAATATTTA ATGGAATCCC AGACTGGGTT 
TACGAAGAGG AAATGCTTGC TACAAAATAT GCTCTCTGGT GGTCTCCTAA TGGAAAATTT 
TTGGCGTATG CAGAATTTAA TGACACAGAG ATACCAGTTA TTGCCTATTC CTATTATGGT 
GATGAACAAT ATCCTAGAAC AATAAATATT CCATACCCAA AGGCTGGAGC TAAGAATCCT 
GTTGTTCGGA TCTTTATCAT TGATGCCACT TACCCTGAGC ATATAGGTCC CAGAGAAGTG 
CCAGTACCAG CAATGATAGC CTCAAGTGAT TATTATTTCA GTTGGCTCAC ATGGGTTACT 
GATGATCGAA TATGTTTGCA GTGGCTAAAA AGAATCCAAA ATGTTTCAGT GTTGTCTACA 
TGTGATTTCA GGGAAGACTG GCAGACATGG AACTGTCCAA AGACCCAGGA GCATATAGAA 
GAAAGCAGAA CTGGATGGGC TGGTGGATTC TTTGTTTCAA CACCAGTGTT CAGCCATGAT 
ACCATTTCAT ACTACAAAAT ATTTAGTGAC AAGGATGGCT ACAAACATAT TCACTATATC 
AGAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATAAATATA 
TTCAGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG CTACCCTGGA 
AGAAGAAATA TCTATAGAAT TAGCATTGGA AGCCATTCTC CAAGCAAGAA GTGCATTACT 
TGCCATCTAA GGAAAAAAAG GTGCCAATAT TACACAGCAA GTTTCAGCGA CTATGCCAAG 
TACTACGCAC TCGTCTGCTA TGGCCCAGGC CTCCCCATTT CCACCCTTCA TGATGGCCGC 
ACCGACCAAG AAATTAAAAT CCTGGAAGAC AACAAGGAAT TGGAAAATGC TTTGAAAAAT 
ATTCAGCTGC CCAAAGAAGA AATTAAGAAA CTTAAAGTGG ATGATATTAC ATTGTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAAA TCAAAGAAGT ATCCCTTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGTCA GAGTGTAAGG TCTATATTCG CTGTTAGTTG GATATCTTAT 
CTTGCAAGTA AGGAAGGGAT AGTCATTGCC TTGGTGGATG GTCGAGGAAC AGCTTTCCAA 
GGTGACAAAC TCCTGTATGC AGTGTATCGA AAGCTGGGTG TTTATGAAGT TGAGGACCAG 
ATCACAGCTG TCAGTCCTAT GGAGGTTATG TTTCATCGCT GGCCCTTGCT TCAGGAACTG 
GTCTTTTCAA ATGTGGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800271.1
CDS	382..2262
Translation	MKTWLKIVFGVATSAVLALLVMCIVLRPSRVHNSEESTTRALTLKDILNGTFSYKTFFPNWISGQEYLHQSTDNNVVFYNIETGESYTILSNTTMKSVNASNYGLSPDRQFAYLESDYSKLWRYSYTATYHIYDLTNGEFIRRNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPEDPPFQITYNGKENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTEIPVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDATYPEHIGPREVPVPAMIASSDYYFSWLTWVTDDRICLQWLKRIQNVSVLSTCDFREDWQTWNCPKTQEHIEESRTGWAGGFFVSTPVFSHDTISYYKIFSDKDGYKHIHYIRDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGSHSPSKKCITCHLRKKRCQYYTASFSDYAKYYALVCYGPGLPISTLHDGRTDQEIKILEDNKELENALKNIQLPKEEIKKLKVDDITLWYKMILPPQFDKSKKYPLLIQVYGGPCSQSVRSIFAVSWISYLASKEGIVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVSPMEVMFHRWPLLQELVFSNVG

Target ORF information:

RefSeq Version	XM_010801969.3
Organism	Bos taurus(cattle)
Definition	Bos taurus fibroblast activation protein alpha (FAP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801969.3

ORF Insert Sequence:

ATGAAGACAT GGTTAAAAAT TGTATTTGGA GTTGCCACCT CTGCCGTGCT TGCTTTACTG 
GTGATGTGTA TTGTCTTACG TCCTTCAAGA GTTCACAACT CTGAAGAGAG TACAACACGA 
GCACTCACAC TGAAGGATAT TTTAAATGGG ACATTTTCTT ATAAAACATT TTTTCCAAAT 
TGGATTTCAG GACAAGAATA TCTTCATCAA TCTACAGATA ATAATGTAGT ATTTTATAAT 
ATTGAAACAG GAGAATCATA TACCATTTTG AGTAATACCA CCATGAAAAG TGTGAATGCT 
TCAAATTATG GTTTATCACC TGATCGGCAA TTTGCATACC TAGAAAGTGA TTATTCAAAG 
CTTTGGAGAT ACTCTTACAC AGCAACATAT CACATCTACG ACCTTACAAA CGGGGAGTTT 
ATAAGAAGAA ATGAGCTTCC TCGTCCAATT CAGTATTTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATC TATCTGAAAC AAAGACCAGA AGACCCACCT 
TTTCAAATAA CATATAATGG AAAAGAAAAT AAAATATTTA ATGGAATCCC AGACTGGGTT 
TACGAAGAGG AAATGCTTGC TACAAAATAT GCTCTCTGGT GGTCTCCTAA TGGAAAATTT 
TTGGCGTATG CAGAATTTAA TGACACAGAG ATACCAGTTA TTGCCTATTC CTATTATGGT 
GATGAACAAT ATCCTAGAAC AATAAATATT CCATACCCAA AGGCTGGAGC TAAGAATCCT 
GTTGTTCGGA TCTTTATCAT TGATGCCACT TACCCTGAGC ATATAGGTCC CAGAGAAGTG 
CCAGTACCAG CAATGATAGC CTCAAGTGAT TATTATTTCA GTTGGCTCAC ATGGGTTACT 
GATGATCGAA TATGTTTGCA GTGGCTAAAA AGAATCCAAA ATGTTTCAGT GTTGTCTACA 
TGTGATTTCA GGGAAGACTG GCAGACATGG AACTGTCCAA AGACCCAGGA GCATATAGAA 
GAAAGCAGAA CTGGATGGGC TGGTGGATTC TTTGTTTCAA CACCAGTGTT CAGCCATGAT 
ACCATTTCAT ACTACAAAAT ATTTAGTGAC AAGGATGGCT ACAAACATAT TCACTATATC 
AGAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATAAATATA 
TTCAGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG CTACCCTGGA 
AGAAGAAATA TCTATAGAAT TAGCATTGGA AGCCATTCTC CAAGCAAGAA GTGCATTACT 
TGCCATCTAA GGAAAAAAAG GTGCCAATAT TACACAGCAA GTTTCAGCGA CTATGCCAAG 
TACTACGCAC TCGTCTGCTA TGGCCCAGGC CTCCCCATTT CCACCCTTCA TGATGGCCGC 
ACCGACCAAG AAATTAAAAT CCTGGAAGAC AACAAGGAAT TGGAAAATGC TTTGAAAAAT 
ATTCAGCTGC CCAAAGAAGA AATTAAGAAA CTTAAAGTGG ATGATATTAC ATTGTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAAA TCAAAGAAGT ATCCCTTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGTCA GAGTGTAAGG TCTATATTCG CTGTTAGTTG GATATCTTAT 
CTTGCAAGTA AGGAAGGGAT AGTCATTGCC TTGGTGGATG GTCGAGGAAC AGCTTTCCAA 
GGTGACAAAC TCCTGTATGC AGTGTATCGA AAGCTGGGTG TTTATGAAGT TGAGGACCAG 
ATCACAGCTG TCAGTCCTAT GGAGGTTATG TTTCATCGCT GGCCCTTGCT TCAGGAACTG 
GTCTTTTCAA ATGTGGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAP ( NM_001098001.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001091470.1 Bos taurus fibroblast activation protein alpha (FAP), mRNA.

Price & Availability↑

CloneID	OBa02333
Clone ID Related Accession (Same CDS sequence)	NM_001098001.1
Accession Version	NM_001098001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	prolyl endopeptidase FAP
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC140497.1. On May 24, 2007 this sequence version replaced XM_585727.3. ##Evidence-Data-START## Transcript exon combination :: BC140497.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145436 [ECO:0000348] ##Evidence-Data-END##

ATGAAGACAT GGTTAAAAAT TGTATTTGGA GTTGCCACCT CTGCCGTGCT TGCTTTACTG 
GTGATGTGTA TTGTCTTACG TCCTTCAAGA GTTCACAACT CTGAAGAGAG TACAACACGA 
GCACTCACAC TGAAGGATAT TTTAAATGGG ACATTTTCTT ATAAAACATT TTTTCCAAAT 
TGGATTTCAG GACAAGAATA TCTTCATCAA TCTACAGATA ATAATGTAGT ATTTTATAAT 
ATTGAAACAG GAGAATCATA TACCATTTTG AGTAATACCA CCATGAAAAG TGTGAATGCT 
TCAAATTATG GTTTATCACC TGATCGGCAA TTTGCATACC TAGAAAGTGA TTATTCAAAG 
CTTTGGAGAT ACTCTTACAC AGCAACATAT CACATCTACG ACCTTACAAA CGGGGAGTTT 
ATAAGAAGAA ATGAGCTTCC TCGTCCAATT CAGTATTTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATC TATCTGAAAC AAAGACCAGA AGACCCACCT 
TTTCAAATAA CATATAATGG AAAAGAAAAT AAAATATTTA ATGGAATCCC AGACTGGGTT 
TACGAAGAGG AAATGCTTGC TACAAAATAT GCTCTCTGGT GGTCTCCTAA TGGAAAATTT 
TTGGCGTATG CAGAATTTAA TGACACAGAG ATACCAGTTA TTGCCTATTC CTATTATGGT 
GATGAACAAT ATCCTAGAAC AATAAATATT CCATACCCAA AGGCTGGAGC TAAGAATCCT 
GTTGTTCGGA TCTTTATCAT TGATGCCACT TACCCTGAGC ATATAGGTCC CAGAGAAGTG 
CCAGTACCAG CAATGATAGC CTCAAGTGAT TATTATTTCA GTTGGCTCAC ATGGGTTACT 
GATGATCGAA TATGTTTGCA GTGGCTAAAA AGAATCCAAA ATGTTTCAGT GTTGTCTACA 
TGTGATTTCA GGGAAGACTG GCAGACATGG AACTGTCCAA AGACCCAGGA GCATATAGAA 
GAAAGCAGAA CTGGATGGGC TGGTGGATTC TTTGTTTCAA CACCAGTGTT CAGCCATGAT 
ACCATTTCAT ACTACAAAAT ATTTAGTGAC AAGGATGGCT ACAAACATAT TCACTATATC 
AGAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATAAATATA 
TTCAGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG CTACCCTGGA 
AGAAGAAATA TCTATAGAAT TAGCATTGGA AGCCATTCTC CAAGCAAGAA GTGCATTACT 
TGCCATCTAA GGAAAAAAAG GTGCCAATAT TACACAGCAA GTTTCAGCGA CTATGCCAAG 
TACTACGCAC TCGTCTGCTA TGGCCCAGGC CTCCCCATTT CCACCCTTCA TGATGGCCGC 
ACCGACCAAG AAATTAAAAT CCTGGAAGAC AACAAGGAAT TGGAAAATGC TTTGAAAAAT 
ATTCAGCTGC CCAAAGAAGA AATTAAGAAA CTTAAAGTGG ATGATATTAC ATTGTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAAA TCAAAGAAGT ATCCCTTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGTCA GAGTGTAAGG TCTATATTCG CTGTTAGTTG GATATCTTAT 
CTTGCAAGTA AGGAAGGGAT AGTCATTGCC TTGGTGGATG GTCGAGGAAC AGCTTTCCAA 
GGTGACAAAC TCCTGTATGC AGTGTATCGA AAGCTGGGTG TTTATGAAGT TGAGGACCAG 
ATCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAAAAAG AATAGCCATA 
TGGGGCTGGT CCTATGGAGG TTATGTTTCA TCGCTGGCCC TTGCTTCAGG AACTGGTCTT 
TTCAAATGTG GGATAGCGGT GGCTCCGGTC TCCAGCTGGG AATATTACGC ATCTATCTAC 
ACAGAGCGAT TCATGGGCCT CCCAACAAAG GATGATAATC TCAAGCACTA TAAAAATTCA 
ACTGTGATGG CAAGAGCAGA ATATTTCAGA AATGTAGACT ATCTTCTCAT CCACGGAACA 
GCAGATGATA ATGTGCACTT TCAAAACTCA GCGCAGATTG CTAAAGCTCT GGTTAATGCA 
CAAGTGGATT TCCAGGCAAT GTGGTACTCG GACCAGAACC ATGGCCTATC CGGCCTATCT 
ACGAAGCACT TATACACTCA CATGACCCAC TTCCTAAAGC AGTGTTTTTC TTTGTCAGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091470.1
CDS	387..2669
Translation	MKTWLKIVFGVATSAVLALLVMCIVLRPSRVHNSEESTTRALTLKDILNGTFSYKTFFPNWISGQEYLHQSTDNNVVFYNIETGESYTILSNTTMKSVNASNYGLSPDRQFAYLESDYSKLWRYSYTATYHIYDLTNGEFIRRNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPEDPPFQITYNGKENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTEIPVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDATYPEHIGPREVPVPAMIASSDYYFSWLTWVTDDRICLQWLKRIQNVSVLSTCDFREDWQTWNCPKTQEHIEESRTGWAGGFFVSTPVFSHDTISYYKIFSDKDGYKHIHYIRDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGSHSPSKKCITCHLRKKRCQYYTASFSDYAKYYALVCYGPGLPISTLHDGRTDQEIKILEDNKELENALKNIQLPKEEIKKLKVDDITLWYKMILPPQFDKSKKYPLLIQVYGGPCSQSVRSIFAVSWISYLASKEGIVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYTERFMGLPTKDDNLKHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGLSGLSTKHLYTHMTHFLKQCFSLSD

Target ORF information:

RefSeq Version	NM_001098001.1
Organism	Bos taurus(cattle)
Definition	Bos taurus fibroblast activation protein alpha (FAP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098001.1

ORF Insert Sequence:

ATGAAGACAT GGTTAAAAAT TGTATTTGGA GTTGCCACCT CTGCCGTGCT TGCTTTACTG 
GTGATGTGTA TTGTCTTACG TCCTTCAAGA GTTCACAACT CTGAAGAGAG TACAACACGA 
GCACTCACAC TGAAGGATAT TTTAAATGGG ACATTTTCTT ATAAAACATT TTTTCCAAAT 
TGGATTTCAG GACAAGAATA TCTTCATCAA TCTACAGATA ATAATGTAGT ATTTTATAAT 
ATTGAAACAG GAGAATCATA TACCATTTTG AGTAATACCA CCATGAAAAG TGTGAATGCT 
TCAAATTATG GTTTATCACC TGATCGGCAA TTTGCATACC TAGAAAGTGA TTATTCAAAG 
CTTTGGAGAT ACTCTTACAC AGCAACATAT CACATCTACG ACCTTACAAA CGGGGAGTTT 
ATAAGAAGAA ATGAGCTTCC TCGTCCAATT CAGTATTTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATC TATCTGAAAC AAAGACCAGA AGACCCACCT 
TTTCAAATAA CATATAATGG AAAAGAAAAT AAAATATTTA ATGGAATCCC AGACTGGGTT 
TACGAAGAGG AAATGCTTGC TACAAAATAT GCTCTCTGGT GGTCTCCTAA TGGAAAATTT 
TTGGCGTATG CAGAATTTAA TGACACAGAG ATACCAGTTA TTGCCTATTC CTATTATGGT 
GATGAACAAT ATCCTAGAAC AATAAATATT CCATACCCAA AGGCTGGAGC TAAGAATCCT 
GTTGTTCGGA TCTTTATCAT TGATGCCACT TACCCTGAGC ATATAGGTCC CAGAGAAGTG 
CCAGTACCAG CAATGATAGC CTCAAGTGAT TATTATTTCA GTTGGCTCAC ATGGGTTACT 
GATGATCGAA TATGTTTGCA GTGGCTAAAA AGAATCCAAA ATGTTTCAGT GTTGTCTACA 
TGTGATTTCA GGGAAGACTG GCAGACATGG AACTGTCCAA AGACCCAGGA GCATATAGAA 
GAAAGCAGAA CTGGATGGGC TGGTGGATTC TTTGTTTCAA CACCAGTGTT CAGCCATGAT 
ACCATTTCAT ACTACAAAAT ATTTAGTGAC AAGGATGGCT ACAAACATAT TCACTATATC 
AGAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATAAATATA 
TTCAGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG CTACCCTGGA 
AGAAGAAATA TCTATAGAAT TAGCATTGGA AGCCATTCTC CAAGCAAGAA GTGCATTACT 
TGCCATCTAA GGAAAAAAAG GTGCCAATAT TACACAGCAA GTTTCAGCGA CTATGCCAAG 
TACTACGCAC TCGTCTGCTA TGGCCCAGGC CTCCCCATTT CCACCCTTCA TGATGGCCGC 
ACCGACCAAG AAATTAAAAT CCTGGAAGAC AACAAGGAAT TGGAAAATGC TTTGAAAAAT 
ATTCAGCTGC CCAAAGAAGA AATTAAGAAA CTTAAAGTGG ATGATATTAC ATTGTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAAA TCAAAGAAGT ATCCCTTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGTCA GAGTGTAAGG TCTATATTCG CTGTTAGTTG GATATCTTAT 
CTTGCAAGTA AGGAAGGGAT AGTCATTGCC TTGGTGGATG GTCGAGGAAC AGCTTTCCAA 
GGTGACAAAC TCCTGTATGC AGTGTATCGA AAGCTGGGTG TTTATGAAGT TGAGGACCAG 
ATCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAAAAAG AATAGCCATA 
TGGGGCTGGT CCTATGGAGG TTATGTTTCA TCGCTGGCCC TTGCTTCAGG AACTGGTCTT 
TTCAAATGTG GGATAGCGGT GGCTCCGGTC TCCAGCTGGG AATATTACGC ATCTATCTAC 
ACAGAGCGAT TCATGGGCCT CCCAACAAAG GATGATAATC TCAAGCACTA TAAAAATTCA 
ACTGTGATGG CAAGAGCAGA ATATTTCAGA AATGTAGACT ATCTTCTCAT CCACGGAACA 
GCAGATGATA ATGTGCACTT TCAAAACTCA GCGCAGATTG CTAAAGCTCT GGTTAATGCA 
CAAGTGGATT TCCAGGCAAT GTGGTACTCG GACCAGAACC ATGGCCTATC CGGCCTATCT 
ACGAAGCACT TATACACTCA CATGACCCAC TTCCTAAAGC AGTGTTTTTC TTTGTCAGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAP ( XM_010801968.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800270.1 Bos taurus fibroblast activation protein alpha (FAP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa47278
Clone ID Related Accession (Same CDS sequence)	XM_010801968.2 , XM_010801968.3
Accession Version	XM_010801968.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	prolyl endopeptidase FAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAT GGTTAAAAAT TGTATTTGGA GTTGCCACCT CTGCCGTGCT TGCTTTACTG 
GTGATGTGTA TTGTCTTACG TCCTTCAAGA GTTCACAACT CTGAAGAGAG TACAACACGA 
GCACTCACAC TGAAGGATAT TTTAAATGGG ACATTTTCTT ATAAAACATT TTTTCCAAAT 
TGGATTTCAG GACAAGAATA TCTTCATCAA TCTACAGATA ATAATGTAGT ATTTTATAAT 
ATTGAAACAG GAGAATCATA TACCATTTTG AGTAATACCA CCATGAAAAG TGTGAATGCT 
TCAAATTATG GTTTATCACC TGATCGGCAA TTTGCATACC TAGAAAGTGA TTATTCAAAG 
CTTTGGAGAT ACTCTTACAC AGCAACATAT CACATCTACG ACCTTACAAA CGGGGAGTTT 
ATAAGAAGAA ATGAGCTTCC TCGTCCAATT CAGTATTTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATC TATCTGAAAC AAAGACCAGA AGACCCACCT 
TTTCAAATAA CATATAATGG AAAAGAAAAT AAAATATTTA ATGGAATCCC AGACTGGGTT 
TACGAAGAGG AAATGCTTGC TACAAAATAT GCTCTCTGGT GGTCTCCTAA TGGAAAATTT 
TTGGCGTATG CAGAATTTAA TGACACAGAG ATACCAGTTA TTGCCTATTC CTATTATGGT 
GATGAACAAT ATCCTAGAAC AATAAATATT CCATACCCAA AGGCTGGAGC TAAGAATCCT 
GTTGTTCGGA TCTTTATCAT TGATGCCACT TACCCTGAGC ATATAGGTCC CAGAGAAGTG 
CCAGTACCAG CAATGATAGC CTCAAGTGAT TATTATTTCA GTTGGCTCAC ATGGGTTACT 
GATGATCGAA TATGTTTGCA GTGGCTAAAA AGAATCCAAA ATGTTTCAGT GTTGTCTACA 
TGTGATTTCA GGGAAGACTG GCAGACATGG AACTGTCCAA AGACCCAGGA GCATATAGAA 
GAAAGCAGAA CTGGATGGGC TGGTGGATTC TTTGTTTCAA CACCAGTGTT CAGCCATGAT 
ACCATTTCAT ACTACAAAAT ATTTAGTGAC AAGGATGGCT ACAAACATAT TCACTATATC 
AGAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATAAATATA 
TTCAGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG CTACCCTGGA 
AGAAGAAATA TCTATAGAAT TAGCATTGGA AGCCATTCTC CAAGCAAGAA GTGCATTACT 
TGCCATCTAA GGAAAAAAAG GTGCCAATAT TACACAGCAA GTTTCAGCGA CTATGCCAAG 
TACTACGCAC TCGTCTGCTA TGGCCCAGGC CTCCCCATTT CCACCCTTCA TGATGGCCGC 
ACCGACCAAG AAATTAAAAT CCTGGAAGAC AACAAGGAAT TGGAAAATGC TTTGAAAAAT 
ATTCAGCTGC CCAAAGAAGA AATTAAGAAA CTTAAAGTGG ATGATATTAC ATTGTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAAA TCAAAGAAGT ATCCCTTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGTCA GAGTGTAAGG TCTATATTCG CTGTTAGTTG GATATCTTAT 
CTTGCAAGTA AGGAAGGGAT AGTCATTGCC TTGGTGGATG GTCGAGGAAC AGCTTTCCAA 
GGTGACAAAC TCCTGTATGC AGTGTATCGA AAGCTGGGTG TTTATGAAGT TGAGGACCAG 
ATCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAAAAAG AATAGCCATA 
TGGGGCTGGT CCTATGGAGG TTATGTTTCA TCGCTGGCCC TTGCTTCAGG AACTGGTCTT 
TTCAAATGTG GGATAGCGGT GGCTCCGGTC TCCAGCTGGG AATATTACGG TGCTCCTGTT 
TCCACAATCT TCTCCTGCAT CTCTTCTCCA GGCTTGTTGA GACAACTAGC AGAGCAAATG 
ACTAGGGTGG AGCATCACAG AAAGGAAAGC AAGACCTTTC CTCAGCAGCT CCCCCTGGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800270.1
CDS	414..2519
Translation	MKTWLKIVFGVATSAVLALLVMCIVLRPSRVHNSEESTTRALTLKDILNGTFSYKTFFPNWISGQEYLHQSTDNNVVFYNIETGESYTILSNTTMKSVNASNYGLSPDRQFAYLESDYSKLWRYSYTATYHIYDLTNGEFIRRNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPEDPPFQITYNGKENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTEIPVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDATYPEHIGPREVPVPAMIASSDYYFSWLTWVTDDRICLQWLKRIQNVSVLSTCDFREDWQTWNCPKTQEHIEESRTGWAGGFFVSTPVFSHDTISYYKIFSDKDGYKHIHYIRDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGSHSPSKKCITCHLRKKRCQYYTASFSDYAKYYALVCYGPGLPISTLHDGRTDQEIKILEDNKELENALKNIQLPKEEIKKLKVDDITLWYKMILPPQFDKSKKYPLLIQVYGGPCSQSVRSIFAVSWISYLASKEGIVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYGAPVSTIFSCISSPGLLRQLAEQMTRVEHHRKESKTFPQQLPLGD

Target ORF information:

RefSeq Version	XM_010801968.2
Organism	Bos taurus(cattle)
Definition	Bos taurus fibroblast activation protein alpha (FAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801968.2

ORF Insert Sequence:

ATGAAGACAT GGTTAAAAAT TGTATTTGGA GTTGCCACCT CTGCCGTGCT TGCTTTACTG 
GTGATGTGTA TTGTCTTACG TCCTTCAAGA GTTCACAACT CTGAAGAGAG TACAACACGA 
GCACTCACAC TGAAGGATAT TTTAAATGGG ACATTTTCTT ATAAAACATT TTTTCCAAAT 
TGGATTTCAG GACAAGAATA TCTTCATCAA TCTACAGATA ATAATGTAGT ATTTTATAAT 
ATTGAAACAG GAGAATCATA TACCATTTTG AGTAATACCA CCATGAAAAG TGTGAATGCT 
TCAAATTATG GTTTATCACC TGATCGGCAA TTTGCATACC TAGAAAGTGA TTATTCAAAG 
CTTTGGAGAT ACTCTTACAC AGCAACATAT CACATCTACG ACCTTACAAA CGGGGAGTTT 
ATAAGAAGAA ATGAGCTTCC TCGTCCAATT CAGTATTTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATC TATCTGAAAC AAAGACCAGA AGACCCACCT 
TTTCAAATAA CATATAATGG AAAAGAAAAT AAAATATTTA ATGGAATCCC AGACTGGGTT 
TACGAAGAGG AAATGCTTGC TACAAAATAT GCTCTCTGGT GGTCTCCTAA TGGAAAATTT 
TTGGCGTATG CAGAATTTAA TGACACAGAG ATACCAGTTA TTGCCTATTC CTATTATGGT 
GATGAACAAT ATCCTAGAAC AATAAATATT CCATACCCAA AGGCTGGAGC TAAGAATCCT 
GTTGTTCGGA TCTTTATCAT TGATGCCACT TACCCTGAGC ATATAGGTCC CAGAGAAGTG 
CCAGTACCAG CAATGATAGC CTCAAGTGAT TATTATTTCA GTTGGCTCAC ATGGGTTACT 
GATGATCGAA TATGTTTGCA GTGGCTAAAA AGAATCCAAA ATGTTTCAGT GTTGTCTACA 
TGTGATTTCA GGGAAGACTG GCAGACATGG AACTGTCCAA AGACCCAGGA GCATATAGAA 
GAAAGCAGAA CTGGATGGGC TGGTGGATTC TTTGTTTCAA CACCAGTGTT CAGCCATGAT 
ACCATTTCAT ACTACAAAAT ATTTAGTGAC AAGGATGGCT ACAAACATAT TCACTATATC 
AGAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATAAATATA 
TTCAGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG CTACCCTGGA 
AGAAGAAATA TCTATAGAAT TAGCATTGGA AGCCATTCTC CAAGCAAGAA GTGCATTACT 
TGCCATCTAA GGAAAAAAAG GTGCCAATAT TACACAGCAA GTTTCAGCGA CTATGCCAAG 
TACTACGCAC TCGTCTGCTA TGGCCCAGGC CTCCCCATTT CCACCCTTCA TGATGGCCGC 
ACCGACCAAG AAATTAAAAT CCTGGAAGAC AACAAGGAAT TGGAAAATGC TTTGAAAAAT 
ATTCAGCTGC CCAAAGAAGA AATTAAGAAA CTTAAAGTGG ATGATATTAC ATTGTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAAA TCAAAGAAGT ATCCCTTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGTCA GAGTGTAAGG TCTATATTCG CTGTTAGTTG GATATCTTAT 
CTTGCAAGTA AGGAAGGGAT AGTCATTGCC TTGGTGGATG GTCGAGGAAC AGCTTTCCAA 
GGTGACAAAC TCCTGTATGC AGTGTATCGA AAGCTGGGTG TTTATGAAGT TGAGGACCAG 
ATCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAAAAAG AATAGCCATA 
TGGGGCTGGT CCTATGGAGG TTATGTTTCA TCGCTGGCCC TTGCTTCAGG AACTGGTCTT 
TTCAAATGTG GGATAGCGGT GGCTCCGGTC TCCAGCTGGG AATATTACGG TGCTCCTGTT 
TCCACAATCT TCTCCTGCAT CTCTTCTCCA GGCTTGTTGA GACAACTAGC AGAGCAAATG 
ACTAGGGTGG AGCATCACAG AAAGGAAAGC AAGACCTTTC CTCAGCAGCT CCCCCTGGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAP ( XM_010801968.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800270.1 Bos taurus fibroblast activation protein alpha (FAP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa47278
Clone ID Related Accession (Same CDS sequence)	XM_010801968.2 , XM_010801968.3
Accession Version	XM_010801968.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	prolyl endopeptidase FAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801968.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAT GGTTAAAAAT TGTATTTGGA GTTGCCACCT CTGCCGTGCT TGCTTTACTG 
GTGATGTGTA TTGTCTTACG TCCTTCAAGA GTTCACAACT CTGAAGAGAG TACAACACGA 
GCACTCACAC TGAAGGATAT TTTAAATGGG ACATTTTCTT ATAAAACATT TTTTCCAAAT 
TGGATTTCAG GACAAGAATA TCTTCATCAA TCTACAGATA ATAATGTAGT ATTTTATAAT 
ATTGAAACAG GAGAATCATA TACCATTTTG AGTAATACCA CCATGAAAAG TGTGAATGCT 
TCAAATTATG GTTTATCACC TGATCGGCAA TTTGCATACC TAGAAAGTGA TTATTCAAAG 
CTTTGGAGAT ACTCTTACAC AGCAACATAT CACATCTACG ACCTTACAAA CGGGGAGTTT 
ATAAGAAGAA ATGAGCTTCC TCGTCCAATT CAGTATTTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATC TATCTGAAAC AAAGACCAGA AGACCCACCT 
TTTCAAATAA CATATAATGG AAAAGAAAAT AAAATATTTA ATGGAATCCC AGACTGGGTT 
TACGAAGAGG AAATGCTTGC TACAAAATAT GCTCTCTGGT GGTCTCCTAA TGGAAAATTT 
TTGGCGTATG CAGAATTTAA TGACACAGAG ATACCAGTTA TTGCCTATTC CTATTATGGT 
GATGAACAAT ATCCTAGAAC AATAAATATT CCATACCCAA AGGCTGGAGC TAAGAATCCT 
GTTGTTCGGA TCTTTATCAT TGATGCCACT TACCCTGAGC ATATAGGTCC CAGAGAAGTG 
CCAGTACCAG CAATGATAGC CTCAAGTGAT TATTATTTCA GTTGGCTCAC ATGGGTTACT 
GATGATCGAA TATGTTTGCA GTGGCTAAAA AGAATCCAAA ATGTTTCAGT GTTGTCTACA 
TGTGATTTCA GGGAAGACTG GCAGACATGG AACTGTCCAA AGACCCAGGA GCATATAGAA 
GAAAGCAGAA CTGGATGGGC TGGTGGATTC TTTGTTTCAA CACCAGTGTT CAGCCATGAT 
ACCATTTCAT ACTACAAAAT ATTTAGTGAC AAGGATGGCT ACAAACATAT TCACTATATC 
AGAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATAAATATA 
TTCAGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG CTACCCTGGA 
AGAAGAAATA TCTATAGAAT TAGCATTGGA AGCCATTCTC CAAGCAAGAA GTGCATTACT 
TGCCATCTAA GGAAAAAAAG GTGCCAATAT TACACAGCAA GTTTCAGCGA CTATGCCAAG 
TACTACGCAC TCGTCTGCTA TGGCCCAGGC CTCCCCATTT CCACCCTTCA TGATGGCCGC 
ACCGACCAAG AAATTAAAAT CCTGGAAGAC AACAAGGAAT TGGAAAATGC TTTGAAAAAT 
ATTCAGCTGC CCAAAGAAGA AATTAAGAAA CTTAAAGTGG ATGATATTAC ATTGTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAAA TCAAAGAAGT ATCCCTTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGTCA GAGTGTAAGG TCTATATTCG CTGTTAGTTG GATATCTTAT 
CTTGCAAGTA AGGAAGGGAT AGTCATTGCC TTGGTGGATG GTCGAGGAAC AGCTTTCCAA 
GGTGACAAAC TCCTGTATGC AGTGTATCGA AAGCTGGGTG TTTATGAAGT TGAGGACCAG 
ATCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAAAAAG AATAGCCATA 
TGGGGCTGGT CCTATGGAGG TTATGTTTCA TCGCTGGCCC TTGCTTCAGG AACTGGTCTT 
TTCAAATGTG GGATAGCGGT GGCTCCGGTC TCCAGCTGGG AATATTACGG TGCTCCTGTT 
TCCACAATCT TCTCCTGCAT CTCTTCTCCA GGCTTGTTGA GACAACTAGC AGAGCAAATG 
ACTAGGGTGG AGCATCACAG AAAGGAAAGC AAGACCTTTC CTCAGCAGCT CCCCCTGGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800270.1
CDS	382..2487
Translation	MKTWLKIVFGVATSAVLALLVMCIVLRPSRVHNSEESTTRALTLKDILNGTFSYKTFFPNWISGQEYLHQSTDNNVVFYNIETGESYTILSNTTMKSVNASNYGLSPDRQFAYLESDYSKLWRYSYTATYHIYDLTNGEFIRRNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPEDPPFQITYNGKENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTEIPVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDATYPEHIGPREVPVPAMIASSDYYFSWLTWVTDDRICLQWLKRIQNVSVLSTCDFREDWQTWNCPKTQEHIEESRTGWAGGFFVSTPVFSHDTISYYKIFSDKDGYKHIHYIRDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGSHSPSKKCITCHLRKKRCQYYTASFSDYAKYYALVCYGPGLPISTLHDGRTDQEIKILEDNKELENALKNIQLPKEEIKKLKVDDITLWYKMILPPQFDKSKKYPLLIQVYGGPCSQSVRSIFAVSWISYLASKEGIVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYGAPVSTIFSCISSPGLLRQLAEQMTRVEHHRKESKTFPQQLPLGD

Target ORF information:

RefSeq Version	XM_010801968.3
Organism	Bos taurus(cattle)
Definition	Bos taurus fibroblast activation protein alpha (FAP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801968.3

ORF Insert Sequence:

ATGAAGACAT GGTTAAAAAT TGTATTTGGA GTTGCCACCT CTGCCGTGCT TGCTTTACTG 
GTGATGTGTA TTGTCTTACG TCCTTCAAGA GTTCACAACT CTGAAGAGAG TACAACACGA 
GCACTCACAC TGAAGGATAT TTTAAATGGG ACATTTTCTT ATAAAACATT TTTTCCAAAT 
TGGATTTCAG GACAAGAATA TCTTCATCAA TCTACAGATA ATAATGTAGT ATTTTATAAT 
ATTGAAACAG GAGAATCATA TACCATTTTG AGTAATACCA CCATGAAAAG TGTGAATGCT 
TCAAATTATG GTTTATCACC TGATCGGCAA TTTGCATACC TAGAAAGTGA TTATTCAAAG 
CTTTGGAGAT ACTCTTACAC AGCAACATAT CACATCTACG ACCTTACAAA CGGGGAGTTT 
ATAAGAAGAA ATGAGCTTCC TCGTCCAATT CAGTATTTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTCTATCA AAACAATATC TATCTGAAAC AAAGACCAGA AGACCCACCT 
TTTCAAATAA CATATAATGG AAAAGAAAAT AAAATATTTA ATGGAATCCC AGACTGGGTT 
TACGAAGAGG AAATGCTTGC TACAAAATAT GCTCTCTGGT GGTCTCCTAA TGGAAAATTT 
TTGGCGTATG CAGAATTTAA TGACACAGAG ATACCAGTTA TTGCCTATTC CTATTATGGT 
GATGAACAAT ATCCTAGAAC AATAAATATT CCATACCCAA AGGCTGGAGC TAAGAATCCT 
GTTGTTCGGA TCTTTATCAT TGATGCCACT TACCCTGAGC ATATAGGTCC CAGAGAAGTG 
CCAGTACCAG CAATGATAGC CTCAAGTGAT TATTATTTCA GTTGGCTCAC ATGGGTTACT 
GATGATCGAA TATGTTTGCA GTGGCTAAAA AGAATCCAAA ATGTTTCAGT GTTGTCTACA 
TGTGATTTCA GGGAAGACTG GCAGACATGG AACTGTCCAA AGACCCAGGA GCATATAGAA 
GAAAGCAGAA CTGGATGGGC TGGTGGATTC TTTGTTTCAA CACCAGTGTT CAGCCATGAT 
ACCATTTCAT ACTACAAAAT ATTTAGTGAC AAGGATGGCT ACAAACATAT TCACTATATC 
AGAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATAAATATA 
TTCAGAGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG CTACCCTGGA 
AGAAGAAATA TCTATAGAAT TAGCATTGGA AGCCATTCTC CAAGCAAGAA GTGCATTACT 
TGCCATCTAA GGAAAAAAAG GTGCCAATAT TACACAGCAA GTTTCAGCGA CTATGCCAAG 
TACTACGCAC TCGTCTGCTA TGGCCCAGGC CTCCCCATTT CCACCCTTCA TGATGGCCGC 
ACCGACCAAG AAATTAAAAT CCTGGAAGAC AACAAGGAAT TGGAAAATGC TTTGAAAAAT 
ATTCAGCTGC CCAAAGAAGA AATTAAGAAA CTTAAAGTGG ATGATATTAC ATTGTGGTAC 
AAGATGATTC TTCCTCCTCA GTTTGACAAA TCAAAGAAGT ATCCCTTGCT AATTCAAGTG 
TATGGTGGTC CCTGCAGTCA GAGTGTAAGG TCTATATTCG CTGTTAGTTG GATATCTTAT 
CTTGCAAGTA AGGAAGGGAT AGTCATTGCC TTGGTGGATG GTCGAGGAAC AGCTTTCCAA 
GGTGACAAAC TCCTGTATGC AGTGTATCGA AAGCTGGGTG TTTATGAAGT TGAGGACCAG 
ATCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAAAAAG AATAGCCATA 
TGGGGCTGGT CCTATGGAGG TTATGTTTCA TCGCTGGCCC TTGCTTCAGG AACTGGTCTT 
TTCAAATGTG GGATAGCGGT GGCTCCGGTC TCCAGCTGGG AATATTACGG TGCTCCTGTT 
TCCACAATCT TCTCCTGCAT CTCTTCTCCA GGCTTGTTGA GACAACTAGC AGAGCAAATG 
ACTAGGGTGG AGCATCACAG AAAGGAAAGC AAGACCTTTC CTCAGCAGCT CCCCCTGGGA 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Fibroblast activation protein alpha is expressed by chondrocytes following a pro-inflammatory stimulus and is elevated in osteoarthritis.
Arthritis research & therapy8(1)R23(2006)
Milner JM,Kevorkian L,Young DA,Jones D,Wait R,Donell ST,Barksby E,Patterson AM,Middleton J,Cravatt BF,Clark IM,Rowan AD,Cawston TE

Purification, identification and characterisation of seprase from bovine serum.
The international journal of biochemistry & cell biology36(11)2320-33(2004 Nov)
Collins PJ,McMahon G,O'Brien P,O'Connor B

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FAP cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム