DENND4B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DENND4B
Entrez Gene ID	508196
Full Name	DENN domain containing 4B
General protein information	Preferred Names DENN domain containing 4B Names DENN domain-containing protein 4B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015462579.1	XP_015318065.1	DENN domain-containing protein 4B
XM_024989951.1	XP_024845719.1	DENN domain-containing protein 4B isoform X1
XM_002686018.4	XP_002686064.4	DENN domain-containing protein 4B isoform X4
XM_024989954.1	XP_024845722.1	DENN domain-containing protein 4B isoform X3
XM_024989952.1	XP_024845720.1	DENN domain-containing protein 4B isoform X2
XM_024989953.1	XP_024845721.1	DENN domain-containing protein 4B isoform X2
XM_002686018.3	XP_002686064.3	DENN domain-containing protein 4B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DENND4B	XP_002686064.2
Danio rerio (zebrafish)	LOC798528	XP_002665116.3
Homo sapiens (human)	DENND4B	NP_055671.2
Pan troglodytes (chimpanzee)	DENND4B	XP_513830.3
Mus musculus (house mouse)	Dennd4b	NP_958809.3
Rattus norvegicus (Norway rat)	Dennd4b	XP_342288.5
	LOC718490	XP_002808284.1
Canis lupus familiaris (dog)	DENND4B	XP_537262.4

Gene Ontology

The following DENND4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa233177	XM_024989953.1 Latest version!	Bos taurus DENN domain containing 4B (DENND4B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa233177	XM_024989952.1 Latest version!	Bos taurus DENN domain containing 4B (DENND4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa233178	XM_024989954.1 Latest version!	Bos taurus DENN domain containing 4B (DENND4B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa233179	XM_002686018.4 Latest version!	Bos taurus DENN domain containing 4B (DENND4B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa233176	XM_024989951.1 Latest version!	Bos taurus DENN domain containing 4B (DENND4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DENND4B ( XM_024989952.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845720.1 Bos taurus DENN domain containing 4B (DENND4B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa233177
Clone ID Related Accession (Same CDS sequence)	XM_024989952.1 , XM_024989953.1
Accession Version	XM_024989952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AGCGGCCCCC CCGGCTGGTG GATTACTTCG TGATAGCTGG ACTTGCAGGG 
AACGGAGCAC CCATCCCTGA GGAAACGTGG ATTCCTGAAC CCAGTGGACC CCTGCGCCCT 
CCCCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAGGGCCCT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATCCAGGCT TCCGCGGGGG GCCACCCCTT GGAACTCAGC 
GCTGGGCTCC TAGGTGGAAC TCAACCTGTC ATCTGCTACC GGAGGGGTCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGATA CGACACCCTA TAGCCACTCA GCCAACCTGG CCCCTCCAGG CCCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAGGGGG CAGGGCTGCA TGCCTTGGGC 
ATCACTGACC TCTGCCTGGT ACTGCCCAGC AAGGGCGAGG GCACTCCTCA TACTTACTGC 
CGATTGCCCC GCAACCTCAA CCCCGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCAGTGC CCGTCTTCTG CCTGCCCATG 
GGGGCCACTA TCGAGTGCTG GCCTGCCCAG ACCAAGTACC CCGTGCCCGT CTTCTCCACC 
TTTGTGCTCA CGGGTGCAGC TGGTGATAAG GTGTACGGTG CTGCCCTGCA GTTCTACGAG 
GCGTTCCCGA GGGCCAGGCT GTCGGAGCGG CAGGCTCGGG CCCTGGGCCT GCTGAGTGCT 
GTGGAGCGGG GCCGGGCACT GGGGGGCCGG GCTGTGCGCA GCCGCCGCGC CATCGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCGGCCTTC CGCGCCTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCCG GCCCCCACCG TCTGCCCCTG GAAGCGCACA TCTCCCACTT CATTCACAAT 
GTTCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GCCAGCCTGT ATCCTCACCC CTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA CCCTGGGCCC GGAGCTGGCG ATCACACTGC TGCTTGCTGT GCTCACGGAA 
CACAAGCTAC TAGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTCTG CGAGGCCCTT 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCGCTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGTGC CCCCGTGCCC TTCATCGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCACCTGT GGATGTCATC TGTGTGGATC TTGATACCAA CACGCTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACCCTGC CCCGCAGACC CTACAAGGTT 
CTGCTGGCCA CACTGACACA TCTGTACCAG CAGCTGGATC AGACGTATAC TGGCCCGGAG 
GAGGAGGCCT CCCTGGAATT CCTGCTGACG GACTATGAGG CAGTGTGTGG CCGCCGGGCC 
CGGCTAGAGC GCGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGCCT GCTCAAGGGC 
TACCGGGACT TCCTGCGCCC ACTCACCCAG GCCCCCTCTG AGGGGTCTCG CGATGTTGAC 
AACCTCTTCT TCCTGCAAGG CTTCCTCAAG TCCCGAGAAC GTTCCAGCCA CAAGCTGTAC 
TCCCAGCTGC TACACACACA GATGTTCTCC CAGTTCATCG AGGAATGCTC CTTTGGCTCT 
GCTCGGCATG CTGCCCTTGA ATTCTTTGAC TCTTGTGTCG ACAAGGTCCA CCCAGAGCAG 
GAGAAACCTG AGCCCGTGCC CCTGGTGGAG CTGGAGGAGC TGTCAGGCAG TGAGCTCACC 
GTGTTTATCA TGCCTCCCGA GGAGCCGCCA GCGCCAGAGG GCAGTGAATC CACTCCCCAG 
TACTGTTACG ATGGGTTCCC CGAGCTACGG GCTGAGCTGT TCGAGGCTCC TCAAGAACAA 
GCTGGGGCTC TGCCTGTGCC AGGCCCTTCC CGCAGCGCCC CCAGCAGTCC TGCCCCTCGC 
CGCACCAAAC AGGAGATGAA GGTGGCGCAG CGGATGGCAC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGCCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCCTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCGCTGC AGACGGCCTA CCAGGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCTGACGAGG TGTGTTACCG GGTGCTGATG 
CAGCTGTGCT CGCACTACGG GCAGCCCGTG CTGTCCGTGC GGGTCATGCT GGACATGCGG 
CGGGCGGGCA TCGTGCCCAA CACCATCACC TATGGCTACT ACAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CATCTGGCAC ACCGGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTG 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGCGAGAAC AGCGGCAGCG GCAGCAGAAG 
CGGGAGGAGG TGGAGACAGC AGCCCAAGAG GCAGGCAGCT CGAAGACAGA GCCCTATTTG 
GAGCGCCCCT CCCCTACCCG CCCCCTTCAG CGCCAGACTA CCTGGGCCGG GCGAAGCCTG 
CGGGACCCTG CCTCACCTAT GGGGCGCCTG GTGAAGAGTG GCAGCCTGGG GAGTGCCCGA 
GGGGCACAGC CCACCGTGGA GGCTGGTGTG GCCCACATGA TAGAGGCCTT GGGGGTCCTG 
GAACCCCGGG GATCACCCAT ACCCTGGCAT GATGGAAGCC TCTCAGACCT GAGCCTGACC 
GGGGAAGAGT CGGCACCTGG AGGCAGCCCA GGGGACTCAG GCTCAGCCCT GAGCACCCAG 
TCCACTGAAA CCCTGGAAGG GCCAAGTGGG CGGGTGCCTA AGGCTGGTGG GCGTCAAGAT 
GAGGCCAGCA CCCCCCGAAG AGGGCTGGGC GCCCGCCTCC AACAGCTACT CACTCCTTCC 
CGCCGCTCCC CCACCTCTCG TGCCCCCCCG TCTGAGCTGC CCCCTGACCT GCCTCCCTCA 
GCCCGTCGCA GCCCCATGGA CAGCCTTCTG CACCCCCGGG AGCGCCCTGG ATCCACTGCA 
TCCGAGAGCT CAGCCTCTCT GGGCAGTGAG TGGGACCTCT CAGAATCTTC TCTCAGCAGT 
GTGAGCCTTC GTCGTTCCTC AGAGCGCCTC AGTGACACCC CTGGATCCTT CCAGCCACCT 
TCCCTGGAAA TTCTGCTGTC TAGCTGCTCG CTGTGCCGCG CCTGTGACTC CCTGGTGTAC 
GATGAGGAGA TCATGGCTGG CTGGGCGCCT GACGACTCCA ACCTCAACAC CACCTGTCCG 
TTCTGCGCCT GCCCCTTTGT GCCCCTGCTC AGCGTCCAGA CCCTTGATTC CCGACCCAGC 
GCCCCCAGCC CCAAGCCTGC TCCTGCTGGT GCCAGCGGCA GCAGCAACGC TCCTGTCCCT 
GGGGGCCCAG GCCCTGTGCT CAGTGACCGC AGGCTTTGCC TAGCCCTGGA TGAGCCGCAG 
CTCTGCAACG GGCACATGGG GACTGCCTCC CGGCGTGTTG AGAGTGGGGC ATGGGCGTAT 
CTCAGCCCCC TCGTGCTGCG GAAGGAGCTG GAGTCACTGG TAGAGAACGA GGGCAGTGAG 
GTGCTGGCAT TGCCTGAGTT GCCTGCCGCT CACCCCATCA TCTTCTGGAA CCTTCTGTGG 
TATTTCCAGC GGCTGCGCCT GCCCAGTATT CTGCCATGCT TGGTGCTGGC CTCCCGTGAT 
GGGCCCCCGC CCCACCAGGC CCCAGCTCCT TGGATAATGC CTGATCCAGC ATCCGTGCAG 
GTGCGGCTGC TGTGGGATGT CCTGACCCCT GATCCCGATA GCTGCCCACC TCTCTATGTG 
CTCTGGAGGG TCCACAGCCA GATCCCACAG CGGGTGGTAT GGCCAGGTCC AGTACCTGCA 
TCCCTTAGCC TGGCGTTGCT GGAATCGGTG CTGCGCCACG TCGGTCTCAA CGAGGTGCAC 
AAGGCTATAG GGCTCCTGCT AGAAACTCTA GGGCCCCCTC CCACTGGTCT GCACCTACAA 
AGGGGCATCT ACCGTGAGAT CTTATTCCTG ACACTGGCTG CTCTGGGCAA GGACCACATG 
GATATAGTGG CCTTCGACAA GAAGTACAAA TCCGCCTTTA ACAAGCTGGC CAGCACCATG 
GGCAAGGAGG AGCTGAGGCA GCGGCGGGCA CAGATGCCTA CCCCAAAGGC CATTGATTGC 
CGGAAATGTT TCGGAGCGCC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845720.1
CDS	299..4771
Translation	MAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREEVETAAQEAGSSKTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSNAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASRDGPPPHQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASTMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_024989952.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4B (DENND4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989952.1

ORF Insert Sequence:

ATGGCGGAGG AGCGGCCCCC CCGGCTGGTG GATTACTTCG TGATAGCTGG ACTTGCAGGG 
AACGGAGCAC CCATCCCTGA GGAAACGTGG ATTCCTGAAC CCAGTGGACC CCTGCGCCCT 
CCCCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAGGGCCCT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATCCAGGCT TCCGCGGGGG GCCACCCCTT GGAACTCAGC 
GCTGGGCTCC TAGGTGGAAC TCAACCTGTC ATCTGCTACC GGAGGGGTCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGATA CGACACCCTA TAGCCACTCA GCCAACCTGG CCCCTCCAGG CCCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAGGGGG CAGGGCTGCA TGCCTTGGGC 
ATCACTGACC TCTGCCTGGT ACTGCCCAGC AAGGGCGAGG GCACTCCTCA TACTTACTGC 
CGATTGCCCC GCAACCTCAA CCCCGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCAGTGC CCGTCTTCTG CCTGCCCATG 
GGGGCCACTA TCGAGTGCTG GCCTGCCCAG ACCAAGTACC CCGTGCCCGT CTTCTCCACC 
TTTGTGCTCA CGGGTGCAGC TGGTGATAAG GTGTACGGTG CTGCCCTGCA GTTCTACGAG 
GCGTTCCCGA GGGCCAGGCT GTCGGAGCGG CAGGCTCGGG CCCTGGGCCT GCTGAGTGCT 
GTGGAGCGGG GCCGGGCACT GGGGGGCCGG GCTGTGCGCA GCCGCCGCGC CATCGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCGGCCTTC CGCGCCTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCCG GCCCCCACCG TCTGCCCCTG GAAGCGCACA TCTCCCACTT CATTCACAAT 
GTTCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GCCAGCCTGT ATCCTCACCC CTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA CCCTGGGCCC GGAGCTGGCG ATCACACTGC TGCTTGCTGT GCTCACGGAA 
CACAAGCTAC TAGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTCTG CGAGGCCCTT 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCGCTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGTGC CCCCGTGCCC TTCATCGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCACCTGT GGATGTCATC TGTGTGGATC TTGATACCAA CACGCTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACCCTGC CCCGCAGACC CTACAAGGTT 
CTGCTGGCCA CACTGACACA TCTGTACCAG CAGCTGGATC AGACGTATAC TGGCCCGGAG 
GAGGAGGCCT CCCTGGAATT CCTGCTGACG GACTATGAGG CAGTGTGTGG CCGCCGGGCC 
CGGCTAGAGC GCGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGCCT GCTCAAGGGC 
TACCGGGACT TCCTGCGCCC ACTCACCCAG GCCCCCTCTG AGGGGTCTCG CGATGTTGAC 
AACCTCTTCT TCCTGCAAGG CTTCCTCAAG TCCCGAGAAC GTTCCAGCCA CAAGCTGTAC 
TCCCAGCTGC TACACACACA GATGTTCTCC CAGTTCATCG AGGAATGCTC CTTTGGCTCT 
GCTCGGCATG CTGCCCTTGA ATTCTTTGAC TCTTGTGTCG ACAAGGTCCA CCCAGAGCAG 
GAGAAACCTG AGCCCGTGCC CCTGGTGGAG CTGGAGGAGC TGTCAGGCAG TGAGCTCACC 
GTGTTTATCA TGCCTCCCGA GGAGCCGCCA GCGCCAGAGG GCAGTGAATC CACTCCCCAG 
TACTGTTACG ATGGGTTCCC CGAGCTACGG GCTGAGCTGT TCGAGGCTCC TCAAGAACAA 
GCTGGGGCTC TGCCTGTGCC AGGCCCTTCC CGCAGCGCCC CCAGCAGTCC TGCCCCTCGC 
CGCACCAAAC AGGAGATGAA GGTGGCGCAG CGGATGGCAC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGCCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCCTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCGCTGC AGACGGCCTA CCAGGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCTGACGAGG TGTGTTACCG GGTGCTGATG 
CAGCTGTGCT CGCACTACGG GCAGCCCGTG CTGTCCGTGC GGGTCATGCT GGACATGCGG 
CGGGCGGGCA TCGTGCCCAA CACCATCACC TATGGCTACT ACAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CATCTGGCAC ACCGGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTG 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGCGAGAAC AGCGGCAGCG GCAGCAGAAG 
CGGGAGGAGG TGGAGACAGC AGCCCAAGAG GCAGGCAGCT CGAAGACAGA GCCCTATTTG 
GAGCGCCCCT CCCCTACCCG CCCCCTTCAG CGCCAGACTA CCTGGGCCGG GCGAAGCCTG 
CGGGACCCTG CCTCACCTAT GGGGCGCCTG GTGAAGAGTG GCAGCCTGGG GAGTGCCCGA 
GGGGCACAGC CCACCGTGGA GGCTGGTGTG GCCCACATGA TAGAGGCCTT GGGGGTCCTG 
GAACCCCGGG GATCACCCAT ACCCTGGCAT GATGGAAGCC TCTCAGACCT GAGCCTGACC 
GGGGAAGAGT CGGCACCTGG AGGCAGCCCA GGGGACTCAG GCTCAGCCCT GAGCACCCAG 
TCCACTGAAA CCCTGGAAGG GCCAAGTGGG CGGGTGCCTA AGGCTGGTGG GCGTCAAGAT 
GAGGCCAGCA CCCCCCGAAG AGGGCTGGGC GCCCGCCTCC AACAGCTACT CACTCCTTCC 
CGCCGCTCCC CCACCTCTCG TGCCCCCCCG TCTGAGCTGC CCCCTGACCT GCCTCCCTCA 
GCCCGTCGCA GCCCCATGGA CAGCCTTCTG CACCCCCGGG AGCGCCCTGG ATCCACTGCA 
TCCGAGAGCT CAGCCTCTCT GGGCAGTGAG TGGGACCTCT CAGAATCTTC TCTCAGCAGT 
GTGAGCCTTC GTCGTTCCTC AGAGCGCCTC AGTGACACCC CTGGATCCTT CCAGCCACCT 
TCCCTGGAAA TTCTGCTGTC TAGCTGCTCG CTGTGCCGCG CCTGTGACTC CCTGGTGTAC 
GATGAGGAGA TCATGGCTGG CTGGGCGCCT GACGACTCCA ACCTCAACAC CACCTGTCCG 
TTCTGCGCCT GCCCCTTTGT GCCCCTGCTC AGCGTCCAGA CCCTTGATTC CCGACCCAGC 
GCCCCCAGCC CCAAGCCTGC TCCTGCTGGT GCCAGCGGCA GCAGCAACGC TCCTGTCCCT 
GGGGGCCCAG GCCCTGTGCT CAGTGACCGC AGGCTTTGCC TAGCCCTGGA TGAGCCGCAG 
CTCTGCAACG GGCACATGGG GACTGCCTCC CGGCGTGTTG AGAGTGGGGC ATGGGCGTAT 
CTCAGCCCCC TCGTGCTGCG GAAGGAGCTG GAGTCACTGG TAGAGAACGA GGGCAGTGAG 
GTGCTGGCAT TGCCTGAGTT GCCTGCCGCT CACCCCATCA TCTTCTGGAA CCTTCTGTGG 
TATTTCCAGC GGCTGCGCCT GCCCAGTATT CTGCCATGCT TGGTGCTGGC CTCCCGTGAT 
GGGCCCCCGC CCCACCAGGC CCCAGCTCCT TGGATAATGC CTGATCCAGC ATCCGTGCAG 
GTGCGGCTGC TGTGGGATGT CCTGACCCCT GATCCCGATA GCTGCCCACC TCTCTATGTG 
CTCTGGAGGG TCCACAGCCA GATCCCACAG CGGGTGGTAT GGCCAGGTCC AGTACCTGCA 
TCCCTTAGCC TGGCGTTGCT GGAATCGGTG CTGCGCCACG TCGGTCTCAA CGAGGTGCAC 
AAGGCTATAG GGCTCCTGCT AGAAACTCTA GGGCCCCCTC CCACTGGTCT GCACCTACAA 
AGGGGCATCT ACCGTGAGAT CTTATTCCTG ACACTGGCTG CTCTGGGCAA GGACCACATG 
GATATAGTGG CCTTCGACAA GAAGTACAAA TCCGCCTTTA ACAAGCTGGC CAGCACCATG 
GGCAAGGAGG AGCTGAGGCA GCGGCGGGCA CAGATGCCTA CCCCAAAGGC CATTGATTGC 
CGGAAATGTT TCGGAGCGCC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_024989953.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845721.1 Bos taurus DENN domain containing 4B (DENND4B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa233177
Clone ID Related Accession (Same CDS sequence)	XM_024989952.1 , XM_024989953.1
Accession Version	XM_024989953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AGCGGCCCCC CCGGCTGGTG GATTACTTCG TGATAGCTGG ACTTGCAGGG 
AACGGAGCAC CCATCCCTGA GGAAACGTGG ATTCCTGAAC CCAGTGGACC CCTGCGCCCT 
CCCCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAGGGCCCT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATCCAGGCT TCCGCGGGGG GCCACCCCTT GGAACTCAGC 
GCTGGGCTCC TAGGTGGAAC TCAACCTGTC ATCTGCTACC GGAGGGGTCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGATA CGACACCCTA TAGCCACTCA GCCAACCTGG CCCCTCCAGG CCCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAGGGGG CAGGGCTGCA TGCCTTGGGC 
ATCACTGACC TCTGCCTGGT ACTGCCCAGC AAGGGCGAGG GCACTCCTCA TACTTACTGC 
CGATTGCCCC GCAACCTCAA CCCCGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCAGTGC CCGTCTTCTG CCTGCCCATG 
GGGGCCACTA TCGAGTGCTG GCCTGCCCAG ACCAAGTACC CCGTGCCCGT CTTCTCCACC 
TTTGTGCTCA CGGGTGCAGC TGGTGATAAG GTGTACGGTG CTGCCCTGCA GTTCTACGAG 
GCGTTCCCGA GGGCCAGGCT GTCGGAGCGG CAGGCTCGGG CCCTGGGCCT GCTGAGTGCT 
GTGGAGCGGG GCCGGGCACT GGGGGGCCGG GCTGTGCGCA GCCGCCGCGC CATCGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCGGCCTTC CGCGCCTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCCG GCCCCCACCG TCTGCCCCTG GAAGCGCACA TCTCCCACTT CATTCACAAT 
GTTCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GCCAGCCTGT ATCCTCACCC CTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA CCCTGGGCCC GGAGCTGGCG ATCACACTGC TGCTTGCTGT GCTCACGGAA 
CACAAGCTAC TAGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTCTG CGAGGCCCTT 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCGCTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGTGC CCCCGTGCCC TTCATCGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCACCTGT GGATGTCATC TGTGTGGATC TTGATACCAA CACGCTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACCCTGC CCCGCAGACC CTACAAGGTT 
CTGCTGGCCA CACTGACACA TCTGTACCAG CAGCTGGATC AGACGTATAC TGGCCCGGAG 
GAGGAGGCCT CCCTGGAATT CCTGCTGACG GACTATGAGG CAGTGTGTGG CCGCCGGGCC 
CGGCTAGAGC GCGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGCCT GCTCAAGGGC 
TACCGGGACT TCCTGCGCCC ACTCACCCAG GCCCCCTCTG AGGGGTCTCG CGATGTTGAC 
AACCTCTTCT TCCTGCAAGG CTTCCTCAAG TCCCGAGAAC GTTCCAGCCA CAAGCTGTAC 
TCCCAGCTGC TACACACACA GATGTTCTCC CAGTTCATCG AGGAATGCTC CTTTGGCTCT 
GCTCGGCATG CTGCCCTTGA ATTCTTTGAC TCTTGTGTCG ACAAGGTCCA CCCAGAGCAG 
GAGAAACCTG AGCCCGTGCC CCTGGTGGAG CTGGAGGAGC TGTCAGGCAG TGAGCTCACC 
GTGTTTATCA TGCCTCCCGA GGAGCCGCCA GCGCCAGAGG GCAGTGAATC CACTCCCCAG 
TACTGTTACG ATGGGTTCCC CGAGCTACGG GCTGAGCTGT TCGAGGCTCC TCAAGAACAA 
GCTGGGGCTC TGCCTGTGCC AGGCCCTTCC CGCAGCGCCC CCAGCAGTCC TGCCCCTCGC 
CGCACCAAAC AGGAGATGAA GGTGGCGCAG CGGATGGCAC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGCCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCCTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCGCTGC AGACGGCCTA CCAGGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCTGACGAGG TGTGTTACCG GGTGCTGATG 
CAGCTGTGCT CGCACTACGG GCAGCCCGTG CTGTCCGTGC GGGTCATGCT GGACATGCGG 
CGGGCGGGCA TCGTGCCCAA CACCATCACC TATGGCTACT ACAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CATCTGGCAC ACCGGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTG 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGCGAGAAC AGCGGCAGCG GCAGCAGAAG 
CGGGAGGAGG TGGAGACAGC AGCCCAAGAG GCAGGCAGCT CGAAGACAGA GCCCTATTTG 
GAGCGCCCCT CCCCTACCCG CCCCCTTCAG CGCCAGACTA CCTGGGCCGG GCGAAGCCTG 
CGGGACCCTG CCTCACCTAT GGGGCGCCTG GTGAAGAGTG GCAGCCTGGG GAGTGCCCGA 
GGGGCACAGC CCACCGTGGA GGCTGGTGTG GCCCACATGA TAGAGGCCTT GGGGGTCCTG 
GAACCCCGGG GATCACCCAT ACCCTGGCAT GATGGAAGCC TCTCAGACCT GAGCCTGACC 
GGGGAAGAGT CGGCACCTGG AGGCAGCCCA GGGGACTCAG GCTCAGCCCT GAGCACCCAG 
TCCACTGAAA CCCTGGAAGG GCCAAGTGGG CGGGTGCCTA AGGCTGGTGG GCGTCAAGAT 
GAGGCCAGCA CCCCCCGAAG AGGGCTGGGC GCCCGCCTCC AACAGCTACT CACTCCTTCC 
CGCCGCTCCC CCACCTCTCG TGCCCCCCCG TCTGAGCTGC CCCCTGACCT GCCTCCCTCA 
GCCCGTCGCA GCCCCATGGA CAGCCTTCTG CACCCCCGGG AGCGCCCTGG ATCCACTGCA 
TCCGAGAGCT CAGCCTCTCT GGGCAGTGAG TGGGACCTCT CAGAATCTTC TCTCAGCAGT 
GTGAGCCTTC GTCGTTCCTC AGAGCGCCTC AGTGACACCC CTGGATCCTT CCAGCCACCT 
TCCCTGGAAA TTCTGCTGTC TAGCTGCTCG CTGTGCCGCG CCTGTGACTC CCTGGTGTAC 
GATGAGGAGA TCATGGCTGG CTGGGCGCCT GACGACTCCA ACCTCAACAC CACCTGTCCG 
TTCTGCGCCT GCCCCTTTGT GCCCCTGCTC AGCGTCCAGA CCCTTGATTC CCGACCCAGC 
GCCCCCAGCC CCAAGCCTGC TCCTGCTGGT GCCAGCGGCA GCAGCAACGC TCCTGTCCCT 
GGGGGCCCAG GCCCTGTGCT CAGTGACCGC AGGCTTTGCC TAGCCCTGGA TGAGCCGCAG 
CTCTGCAACG GGCACATGGG GACTGCCTCC CGGCGTGTTG AGAGTGGGGC ATGGGCGTAT 
CTCAGCCCCC TCGTGCTGCG GAAGGAGCTG GAGTCACTGG TAGAGAACGA GGGCAGTGAG 
GTGCTGGCAT TGCCTGAGTT GCCTGCCGCT CACCCCATCA TCTTCTGGAA CCTTCTGTGG 
TATTTCCAGC GGCTGCGCCT GCCCAGTATT CTGCCATGCT TGGTGCTGGC CTCCCGTGAT 
GGGCCCCCGC CCCACCAGGC CCCAGCTCCT TGGATAATGC CTGATCCAGC ATCCGTGCAG 
GTGCGGCTGC TGTGGGATGT CCTGACCCCT GATCCCGATA GCTGCCCACC TCTCTATGTG 
CTCTGGAGGG TCCACAGCCA GATCCCACAG CGGGTGGTAT GGCCAGGTCC AGTACCTGCA 
TCCCTTAGCC TGGCGTTGCT GGAATCGGTG CTGCGCCACG TCGGTCTCAA CGAGGTGCAC 
AAGGCTATAG GGCTCCTGCT AGAAACTCTA GGGCCCCCTC CCACTGGTCT GCACCTACAA 
AGGGGCATCT ACCGTGAGAT CTTATTCCTG ACACTGGCTG CTCTGGGCAA GGACCACATG 
GATATAGTGG CCTTCGACAA GAAGTACAAA TCCGCCTTTA ACAAGCTGGC CAGCACCATG 
GGCAAGGAGG AGCTGAGGCA GCGGCGGGCA CAGATGCCTA CCCCAAAGGC CATTGATTGC 
CGGAAATGTT TCGGAGCGCC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845721.1
CDS	45..4517
Translation	MAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREEVETAAQEAGSSKTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSNAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASRDGPPPHQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASTMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_024989953.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4B (DENND4B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989953.1

ORF Insert Sequence:

ATGGCGGAGG AGCGGCCCCC CCGGCTGGTG GATTACTTCG TGATAGCTGG ACTTGCAGGG 
AACGGAGCAC CCATCCCTGA GGAAACGTGG ATTCCTGAAC CCAGTGGACC CCTGCGCCCT 
CCCCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAGGGCCCT GGGCGAGGAG 
GTGCCCCAGG GCTATACATG CATCCAGGCT TCCGCGGGGG GCCACCCCTT GGAACTCAGC 
GCTGGGCTCC TAGGTGGAAC TCAACCTGTC ATCTGCTACC GGAGGGGTCG TGACAAGCCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGATA CGACACCCTA TAGCCACTCA GCCAACCTGG CCCCTCCAGG CCCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAGGGGG CAGGGCTGCA TGCCTTGGGC 
ATCACTGACC TCTGCCTGGT ACTGCCCAGC AAGGGCGAGG GCACTCCTCA TACTTACTGC 
CGATTGCCCC GCAACCTCAA CCCCGGCATG TGGGGCCCAG CAGTGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACACTGGTG TATGAGGCAG AGCTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCAGTGC CCGTCTTCTG CCTGCCCATG 
GGGGCCACTA TCGAGTGCTG GCCTGCCCAG ACCAAGTACC CCGTGCCCGT CTTCTCCACC 
TTTGTGCTCA CGGGTGCAGC TGGTGATAAG GTGTACGGTG CTGCCCTGCA GTTCTACGAG 
GCGTTCCCGA GGGCCAGGCT GTCGGAGCGG CAGGCTCGGG CCCTGGGCCT GCTGAGTGCT 
GTGGAGCGGG GCCGGGCACT GGGGGGCCGG GCTGTGCGCA GCCGCCGCGC CATCGCTGTG 
CTGTCCCGCT GGCCTGCCTT CCCGGCCTTC CGCGCCTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCCG GCCCCCACCG TCTGCCCCTG GAAGCGCACA TCTCCCACTT CATTCACAAT 
GTTCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCTCC CTATGACAAC 
CTGCTCCTCT GCCAGCCTGT ATCCTCACCC CTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA CCCTGGGCCC GGAGCTGGCG ATCACACTGC TGCTTGCTGT GCTCACGGAA 
CACAAGCTAC TAGTCCACTC GCTGCGGCCA GATCTGCTCA CCAGCGTCTG CGAGGCCCTT 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCGCTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGTGC CCCCGTGCCC TTCATCGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCACCTGT GGATGTCATC TGTGTGGATC TTGATACCAA CACGCTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACCCTGC CCCGCAGACC CTACAAGGTT 
CTGCTGGCCA CACTGACACA TCTGTACCAG CAGCTGGATC AGACGTATAC TGGCCCGGAG 
GAGGAGGCCT CCCTGGAATT CCTGCTGACG GACTATGAGG CAGTGTGTGG CCGCCGGGCC 
CGGCTAGAGC GCGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGCCT GCTCAAGGGC 
TACCGGGACT TCCTGCGCCC ACTCACCCAG GCCCCCTCTG AGGGGTCTCG CGATGTTGAC 
AACCTCTTCT TCCTGCAAGG CTTCCTCAAG TCCCGAGAAC GTTCCAGCCA CAAGCTGTAC 
TCCCAGCTGC TACACACACA GATGTTCTCC CAGTTCATCG AGGAATGCTC CTTTGGCTCT 
GCTCGGCATG CTGCCCTTGA ATTCTTTGAC TCTTGTGTCG ACAAGGTCCA CCCAGAGCAG 
GAGAAACCTG AGCCCGTGCC CCTGGTGGAG CTGGAGGAGC TGTCAGGCAG TGAGCTCACC 
GTGTTTATCA TGCCTCCCGA GGAGCCGCCA GCGCCAGAGG GCAGTGAATC CACTCCCCAG 
TACTGTTACG ATGGGTTCCC CGAGCTACGG GCTGAGCTGT TCGAGGCTCC TCAAGAACAA 
GCTGGGGCTC TGCCTGTGCC AGGCCCTTCC CGCAGCGCCC CCAGCAGTCC TGCCCCTCGC 
CGCACCAAAC AGGAGATGAA GGTGGCGCAG CGGATGGCAC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT GCTGGGCCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCCTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCGCTGC AGACGGCCTA CCAGGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCTGACGAGG TGTGTTACCG GGTGCTGATG 
CAGCTGTGCT CGCACTACGG GCAGCCCGTG CTGTCCGTGC GGGTCATGCT GGACATGCGG 
CGGGCGGGCA TCGTGCCCAA CACCATCACC TATGGCTACT ACAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CATCTGGCAC ACCGGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTG 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGCGAGAAC AGCGGCAGCG GCAGCAGAAG 
CGGGAGGAGG TGGAGACAGC AGCCCAAGAG GCAGGCAGCT CGAAGACAGA GCCCTATTTG 
GAGCGCCCCT CCCCTACCCG CCCCCTTCAG CGCCAGACTA CCTGGGCCGG GCGAAGCCTG 
CGGGACCCTG CCTCACCTAT GGGGCGCCTG GTGAAGAGTG GCAGCCTGGG GAGTGCCCGA 
GGGGCACAGC CCACCGTGGA GGCTGGTGTG GCCCACATGA TAGAGGCCTT GGGGGTCCTG 
GAACCCCGGG GATCACCCAT ACCCTGGCAT GATGGAAGCC TCTCAGACCT GAGCCTGACC 
GGGGAAGAGT CGGCACCTGG AGGCAGCCCA GGGGACTCAG GCTCAGCCCT GAGCACCCAG 
TCCACTGAAA CCCTGGAAGG GCCAAGTGGG CGGGTGCCTA AGGCTGGTGG GCGTCAAGAT 
GAGGCCAGCA CCCCCCGAAG AGGGCTGGGC GCCCGCCTCC AACAGCTACT CACTCCTTCC 
CGCCGCTCCC CCACCTCTCG TGCCCCCCCG TCTGAGCTGC CCCCTGACCT GCCTCCCTCA 
GCCCGTCGCA GCCCCATGGA CAGCCTTCTG CACCCCCGGG AGCGCCCTGG ATCCACTGCA 
TCCGAGAGCT CAGCCTCTCT GGGCAGTGAG TGGGACCTCT CAGAATCTTC TCTCAGCAGT 
GTGAGCCTTC GTCGTTCCTC AGAGCGCCTC AGTGACACCC CTGGATCCTT CCAGCCACCT 
TCCCTGGAAA TTCTGCTGTC TAGCTGCTCG CTGTGCCGCG CCTGTGACTC CCTGGTGTAC 
GATGAGGAGA TCATGGCTGG CTGGGCGCCT GACGACTCCA ACCTCAACAC CACCTGTCCG 
TTCTGCGCCT GCCCCTTTGT GCCCCTGCTC AGCGTCCAGA CCCTTGATTC CCGACCCAGC 
GCCCCCAGCC CCAAGCCTGC TCCTGCTGGT GCCAGCGGCA GCAGCAACGC TCCTGTCCCT 
GGGGGCCCAG GCCCTGTGCT CAGTGACCGC AGGCTTTGCC TAGCCCTGGA TGAGCCGCAG 
CTCTGCAACG GGCACATGGG GACTGCCTCC CGGCGTGTTG AGAGTGGGGC ATGGGCGTAT 
CTCAGCCCCC TCGTGCTGCG GAAGGAGCTG GAGTCACTGG TAGAGAACGA GGGCAGTGAG 
GTGCTGGCAT TGCCTGAGTT GCCTGCCGCT CACCCCATCA TCTTCTGGAA CCTTCTGTGG 
TATTTCCAGC GGCTGCGCCT GCCCAGTATT CTGCCATGCT TGGTGCTGGC CTCCCGTGAT 
GGGCCCCCGC CCCACCAGGC CCCAGCTCCT TGGATAATGC CTGATCCAGC ATCCGTGCAG 
GTGCGGCTGC TGTGGGATGT CCTGACCCCT GATCCCGATA GCTGCCCACC TCTCTATGTG 
CTCTGGAGGG TCCACAGCCA GATCCCACAG CGGGTGGTAT GGCCAGGTCC AGTACCTGCA 
TCCCTTAGCC TGGCGTTGCT GGAATCGGTG CTGCGCCACG TCGGTCTCAA CGAGGTGCAC 
AAGGCTATAG GGCTCCTGCT AGAAACTCTA GGGCCCCCTC CCACTGGTCT GCACCTACAA 
AGGGGCATCT ACCGTGAGAT CTTATTCCTG ACACTGGCTG CTCTGGGCAA GGACCACATG 
GATATAGTGG CCTTCGACAA GAAGTACAAA TCCGCCTTTA ACAAGCTGGC CAGCACCATG 
GGCAAGGAGG AGCTGAGGCA GCGGCGGGCA CAGATGCCTA CCCCAAAGGC CATTGATTGC 
CGGAAATGTT TCGGAGCGCC TCTGGAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_024989954.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845722.1 Bos taurus DENN domain containing 4B (DENND4B), transcript variant X4, mRNA.

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CloneID	OBa233178
Clone ID Related Accession (Same CDS sequence)	XM_024989954.1
Accession Version	XM_024989954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DENN domain-containing protein 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACG 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC CCTGGGCGAG GAGGTGCCCC AGGGCTATAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG TCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTATAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCTTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCCGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG 
GCTGTCGGAG CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC 
ACTGGGGGGC CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC 
CTTCCCGGCC TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CCGGCCCCCA 
CCGTCTGCCC CTGGAAGCAG ACCCCGCATC CTGGTGCAGA TGTCTCCCTA TGACAACCTG 
CTCCTCTGCC AGCCTGTATC CTCACCCCTG CCCCTCAGTG GTGCCAGCTT CCTGCAGCTG 
CTGCAGACCC TGGGCCCGGA GCTGGCGATC ACACTGCTGC TTGCTGTGCT CACGGAACAC 
AAGCTACTAG TCCACTCGCT GCGGCCAGAT CTGCTCACCA GCGTCTGCGA GGCCCTTGTC 
TCTATGATCT TCCCGCTGCA CTGGCAGTGC CCCTACATTC CGCTGTGCCC ACTGGTGCTG 
GCAGATGTGC TGAGTGCCCC CGTGCCCTTC ATCGTGGGTA TCCACTCCAG CTACTTCGAT 
CTGCATGACC CACCTGTGGA TGTCATCTGT GTGGATCTTG ATACCAACAC GCTCTTCCAG 
ACTGAGGAAA AGAAGCCCCT CTCCCCTCGG ACCCTGCCCC GCAGACCCTA CAAGGTTCTG 
CTGGCCACAC TGACACATCT GTACCAGCAG CTGGATCAGA CGTATACTGG CCCGGAGGAG 
GAGGCCTCCC TGGAATTCCT GCTGACGGAC TATGAGGCAG TGTGTGGCCG CCGGGCCCGG 
CTAGAGCGCG AGGTCCAGGG AGCCTTCCTC CGCTTCATGG CCTGCCTGCT CAAGGGCTAC 
CGGGACTTCC TGCGCCCACT CACCCAGGCC CCCTCTGAGG GGTCTCGCGA TGTTGACAAC 
CTCTTCTTCC TGCAAGGCTT CCTCAAGTCC CGAGAACGTT CCAGCCACAA GCTGTACTCC 
CAGCTGCTAC ACACACAGAT GTTCTCCCAG TTCATCGAGG AATGCTCCTT TGGCTCTGCT 
CGGCATGCTG CCCTTGAATT CTTTGACTCT TGTGTCGACA AGGTCCACCC AGAGCAGGAG 
AAACCTGAGC CCGTGCCCCT GGTGGAGCTG GAGGAGCTGT CAGGCAGTGA GCTCACCGTG 
TTTATCATGC CTCCCGAGGA GCCGCCAGCG CCAGAGGGCA GTGAATCCAC TCCCCAGTAC 
TGTTACGATG GGTTCCCCGA GCTACGGGCT GAGCTGTTCG AGGCTCCTCA AGAACAAGCT 
GGGGCTCTGC CTGTGCCAGG CCCTTCCCGC AGCGCCCCCA GCAGTCCTGC CCCTCGCCGC 
ACCAAACAGG AGATGAAGGT GGCGCAGCGG ATGGCACAGA AGTCAGCAGC TGTGCCTGAG 
CTGTGGGCCC GGTGCCTGCT GGGCCACTGC TACGGGCTGT GGTTCCTGTG TCTGCCTGCC 
TACGTGCGCT CGGCGCCCTC CCGGGTGCGG GCGCTGCAGA CGGCCTACCA GGTGCTGCGC 
CAGATGGAGA GCCGCAAGGT GGTGCTGCCT GACGAGGTGT GTTACCGGGT GCTGATGCAG 
CTGTGCTCGC ACTACGGGCA GCCCGTGCTG TCCGTGCGGG TCATGCTGGA CATGCGGCGG 
GCGGGCATCG TGCCCAACAC CATCACCTAT GGCTACTACA ACAAGGCTGT GCTGGAAAGC 
AAGTGGCCAT CTGGCACACC GGGTGGGCGC CTGCGCTGGG CCAAGCTCCG GAACGTGGTC 
CTGGGGGCTG CTCAGTTCCG CCAGCCCTTG CGAGAACAGC GGCAGCGGCA GCAGAAGCGG 
GAGGAGGTGG AGACAGCAGC CCAAGAGGCA GGCAGCTCGA AGACAGAGCC CTATTTGGAG 
CGCCCCTCCC CTACCCGCCC CCTTCAGCGC CAGACTACCT GGGCCGGGCG AAGCCTGCGG 
GACCCTGCCT CACCTATGGG GCGCCTGGTG AAGAGTGGCA GCCTGGGGAG TGCCCGAGGG 
GCACAGCCCA CCGTGGAGGC TGGTGTGGCC CACATGATAG AGGCCTTGGG GGTCCTGGAA 
CCCCGGGGAT CACCCATACC CTGGCATGAT GGAAGCCTCT CAGACCTGAG CCTGACCGGG 
GAAGAGTCGG CACCTGGAGG CAGCCCAGGG GACTCAGGCT CAGCCCTGAG CACCCAGTCC 
ACTGAAACCC TGGAAGGGCC AAGTGGGCGG GTGCCTAAGG CTGGTGGGCG TCAAGATGAG 
GCCAGCACCC CCCGAAGAGG GCTGGGCGCC CGCCTCCAAC AGCTACTCAC TCCTTCCCGC 
CGCTCCCCCA CCTCTCGTGC CCCCCCGTCT GAGCTGCCCC CTGACCTGCC TCCCTCAGCC 
CGTCGCAGCC CCATGGACAG CCTTCTGCAC CCCCGGGAGC GCCCTGGATC CACTGCATCC 
GAGAGCTCAG CCTCTCTGGG CAGTGAGTGG GACCTCTCAG AATCTTCTCT CAGCAGTGTG 
AGCCTTCGTC GTTCCTCAGA GCGCCTCAGT GACACCCCTG GATCCTTCCA GCCACCTTCC 
CTGGAAATTC TGCTGTCTAG CTGCTCGCTG TGCCGCGCCT GTGACTCCCT GGTGTACGAT 
GAGGAGATCA TGGCTGGCTG GGCGCCTGAC GACTCCAACC TCAACACCAC CTGTCCGTTC 
TGCGCCTGCC CCTTTGTGCC CCTGCTCAGC GTCCAGACCC TTGATTCCCG ACCCAGCGCC 
CCCAGCCCCA AGCCTGCTCC TGCTGGTGCC AGCGGCAGCA GCAACGCTCC TGTCCCTGGG 
GGCCCAGGCC CTGTGCTCAG TGACCGCAGG CTTTGCCTAG CCCTGGATGA GCCGCAGCTC 
TGCAACGGGC ACATGGGGAC TGCCTCCCGG CGTGTTGAGA GTGGGGCATG GGCGTATCTC 
AGCCCCCTCG TGCTGCGGAA GGAGCTGGAG TCACTGGTAG AGAACGAGGG CAGTGAGGTG 
CTGGCATTGC CTGAGTTGCC TGCCGCTCAC CCCATCATCT TCTGGAACCT TCTGTGGTAT 
TTCCAGCGGC TGCGCCTGCC CAGTATTCTG CCATGCTTGG TGCTGGCCTC CCGTGATGGG 
CCCCCGCCCC ACCAGGCCCC AGCTCCTTGG ATAATGCCTG ATCCAGCATC CGTGCAGGTG 
CGGCTGCTGT GGGATGTCCT GACCCCTGAT CCCGATAGCT GCCCACCTCT CTATGTGCTC 
TGGAGGGTCC ACAGCCAGAT CCCACAGCGG GTGGTATGGC CAGGTCCAGT ACCTGCATCC 
CTTAGCCTGG CGTTGCTGGA ATCGGTGCTG CGCCACGTCG GTCTCAACGA GGTGCACAAG 
GCTATAGGGC TCCTGCTAGA AACTCTAGGG CCCCCTCCCA CTGGTCTGCA CCTACAAAGG 
GGCATCTACC GTGAGATCTT ATTCCTGACA CTGGCTGCTC TGGGCAAGGA CCACATGGAT 
ATAGTGGCCT TCGACAAGAA GTACAAATCC GCCTTTAACA AGCTGGCCAG CACCATGGGC 
AAGGAGGAGC TGAGGCAGCG GCGGGCACAG ATGCCTACCC CAAAGGCCAT TGATTGCCGG 
AAATGTTTCG GAGCGCCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845722.1
CDS	1010..5299
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAGVRCCPAVLRGVPEGQAVGAAGSGPGPAECCGAGPGTGGPGCAQPPRHRCAVPLACLPGLPRLPHLPLPLLRLRPPPSAPGSRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREEVETAAQEAGSSKTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSNAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASRDGPPPHQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASTMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_024989954.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4B (DENND4B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989954.1

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACG 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC CCTGGGCGAG GAGGTGCCCC AGGGCTATAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG TCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTATAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCTTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCCGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG 
GCTGTCGGAG CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC 
ACTGGGGGGC CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC 
CTTCCCGGCC TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CCGGCCCCCA 
CCGTCTGCCC CTGGAAGCAG ACCCCGCATC CTGGTGCAGA TGTCTCCCTA TGACAACCTG 
CTCCTCTGCC AGCCTGTATC CTCACCCCTG CCCCTCAGTG GTGCCAGCTT CCTGCAGCTG 
CTGCAGACCC TGGGCCCGGA GCTGGCGATC ACACTGCTGC TTGCTGTGCT CACGGAACAC 
AAGCTACTAG TCCACTCGCT GCGGCCAGAT CTGCTCACCA GCGTCTGCGA GGCCCTTGTC 
TCTATGATCT TCCCGCTGCA CTGGCAGTGC CCCTACATTC CGCTGTGCCC ACTGGTGCTG 
GCAGATGTGC TGAGTGCCCC CGTGCCCTTC ATCGTGGGTA TCCACTCCAG CTACTTCGAT 
CTGCATGACC CACCTGTGGA TGTCATCTGT GTGGATCTTG ATACCAACAC GCTCTTCCAG 
ACTGAGGAAA AGAAGCCCCT CTCCCCTCGG ACCCTGCCCC GCAGACCCTA CAAGGTTCTG 
CTGGCCACAC TGACACATCT GTACCAGCAG CTGGATCAGA CGTATACTGG CCCGGAGGAG 
GAGGCCTCCC TGGAATTCCT GCTGACGGAC TATGAGGCAG TGTGTGGCCG CCGGGCCCGG 
CTAGAGCGCG AGGTCCAGGG AGCCTTCCTC CGCTTCATGG CCTGCCTGCT CAAGGGCTAC 
CGGGACTTCC TGCGCCCACT CACCCAGGCC CCCTCTGAGG GGTCTCGCGA TGTTGACAAC 
CTCTTCTTCC TGCAAGGCTT CCTCAAGTCC CGAGAACGTT CCAGCCACAA GCTGTACTCC 
CAGCTGCTAC ACACACAGAT GTTCTCCCAG TTCATCGAGG AATGCTCCTT TGGCTCTGCT 
CGGCATGCTG CCCTTGAATT CTTTGACTCT TGTGTCGACA AGGTCCACCC AGAGCAGGAG 
AAACCTGAGC CCGTGCCCCT GGTGGAGCTG GAGGAGCTGT CAGGCAGTGA GCTCACCGTG 
TTTATCATGC CTCCCGAGGA GCCGCCAGCG CCAGAGGGCA GTGAATCCAC TCCCCAGTAC 
TGTTACGATG GGTTCCCCGA GCTACGGGCT GAGCTGTTCG AGGCTCCTCA AGAACAAGCT 
GGGGCTCTGC CTGTGCCAGG CCCTTCCCGC AGCGCCCCCA GCAGTCCTGC CCCTCGCCGC 
ACCAAACAGG AGATGAAGGT GGCGCAGCGG ATGGCACAGA AGTCAGCAGC TGTGCCTGAG 
CTGTGGGCCC GGTGCCTGCT GGGCCACTGC TACGGGCTGT GGTTCCTGTG TCTGCCTGCC 
TACGTGCGCT CGGCGCCCTC CCGGGTGCGG GCGCTGCAGA CGGCCTACCA GGTGCTGCGC 
CAGATGGAGA GCCGCAAGGT GGTGCTGCCT GACGAGGTGT GTTACCGGGT GCTGATGCAG 
CTGTGCTCGC ACTACGGGCA GCCCGTGCTG TCCGTGCGGG TCATGCTGGA CATGCGGCGG 
GCGGGCATCG TGCCCAACAC CATCACCTAT GGCTACTACA ACAAGGCTGT GCTGGAAAGC 
AAGTGGCCAT CTGGCACACC GGGTGGGCGC CTGCGCTGGG CCAAGCTCCG GAACGTGGTC 
CTGGGGGCTG CTCAGTTCCG CCAGCCCTTG CGAGAACAGC GGCAGCGGCA GCAGAAGCGG 
GAGGAGGTGG AGACAGCAGC CCAAGAGGCA GGCAGCTCGA AGACAGAGCC CTATTTGGAG 
CGCCCCTCCC CTACCCGCCC CCTTCAGCGC CAGACTACCT GGGCCGGGCG AAGCCTGCGG 
GACCCTGCCT CACCTATGGG GCGCCTGGTG AAGAGTGGCA GCCTGGGGAG TGCCCGAGGG 
GCACAGCCCA CCGTGGAGGC TGGTGTGGCC CACATGATAG AGGCCTTGGG GGTCCTGGAA 
CCCCGGGGAT CACCCATACC CTGGCATGAT GGAAGCCTCT CAGACCTGAG CCTGACCGGG 
GAAGAGTCGG CACCTGGAGG CAGCCCAGGG GACTCAGGCT CAGCCCTGAG CACCCAGTCC 
ACTGAAACCC TGGAAGGGCC AAGTGGGCGG GTGCCTAAGG CTGGTGGGCG TCAAGATGAG 
GCCAGCACCC CCCGAAGAGG GCTGGGCGCC CGCCTCCAAC AGCTACTCAC TCCTTCCCGC 
CGCTCCCCCA CCTCTCGTGC CCCCCCGTCT GAGCTGCCCC CTGACCTGCC TCCCTCAGCC 
CGTCGCAGCC CCATGGACAG CCTTCTGCAC CCCCGGGAGC GCCCTGGATC CACTGCATCC 
GAGAGCTCAG CCTCTCTGGG CAGTGAGTGG GACCTCTCAG AATCTTCTCT CAGCAGTGTG 
AGCCTTCGTC GTTCCTCAGA GCGCCTCAGT GACACCCCTG GATCCTTCCA GCCACCTTCC 
CTGGAAATTC TGCTGTCTAG CTGCTCGCTG TGCCGCGCCT GTGACTCCCT GGTGTACGAT 
GAGGAGATCA TGGCTGGCTG GGCGCCTGAC GACTCCAACC TCAACACCAC CTGTCCGTTC 
TGCGCCTGCC CCTTTGTGCC CCTGCTCAGC GTCCAGACCC TTGATTCCCG ACCCAGCGCC 
CCCAGCCCCA AGCCTGCTCC TGCTGGTGCC AGCGGCAGCA GCAACGCTCC TGTCCCTGGG 
GGCCCAGGCC CTGTGCTCAG TGACCGCAGG CTTTGCCTAG CCCTGGATGA GCCGCAGCTC 
TGCAACGGGC ACATGGGGAC TGCCTCCCGG CGTGTTGAGA GTGGGGCATG GGCGTATCTC 
AGCCCCCTCG TGCTGCGGAA GGAGCTGGAG TCACTGGTAG AGAACGAGGG CAGTGAGGTG 
CTGGCATTGC CTGAGTTGCC TGCCGCTCAC CCCATCATCT TCTGGAACCT TCTGTGGTAT 
TTCCAGCGGC TGCGCCTGCC CAGTATTCTG CCATGCTTGG TGCTGGCCTC CCGTGATGGG 
CCCCCGCCCC ACCAGGCCCC AGCTCCTTGG ATAATGCCTG ATCCAGCATC CGTGCAGGTG 
CGGCTGCTGT GGGATGTCCT GACCCCTGAT CCCGATAGCT GCCCACCTCT CTATGTGCTC 
TGGAGGGTCC ACAGCCAGAT CCCACAGCGG GTGGTATGGC CAGGTCCAGT ACCTGCATCC 
CTTAGCCTGG CGTTGCTGGA ATCGGTGCTG CGCCACGTCG GTCTCAACGA GGTGCACAAG 
GCTATAGGGC TCCTGCTAGA AACTCTAGGG CCCCCTCCCA CTGGTCTGCA CCTACAAAGG 
GGCATCTACC GTGAGATCTT ATTCCTGACA CTGGCTGCTC TGGGCAAGGA CCACATGGAT 
ATAGTGGCCT TCGACAAGAA GTACAAATCC GCCTTTAACA AGCTGGCCAG CACCATGGGC 
AAGGAGGAGC TGAGGCAGCG GCGGGCACAG ATGCCTACCC CAAAGGCCAT TGATTGCCGG 
AAATGTTTCG GAGCGCCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_002686018.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002686064.4 Bos taurus DENN domain containing 4B (DENND4B), transcript variant X5, mRNA.

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CloneID	OBa233179
Clone ID Related Accession (Same CDS sequence)	XM_002686018.4
Accession Version	XM_002686018.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DENN domain-containing protein 4B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002686018.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGG CATCACTGAC CTCTGCCTGG TACTGCCCAG CAAGGGCGAG GGCACTCCTC 
ATACTTACTG CCGATTGCCC CGCAACCTCA ACCCCGGCAT GTGGGGCCCA GCAGTGTACC 
TGTGCTACAA GGTGGGCCTG GCCAAGGCCA ACACACTGGT GTATGAGGCA GGTGTACGGT 
GCTGCCCTGC AGTTCTACGA GGCGTTCCCG AGGGCCAGGC TGTCGGAGCG GCAGGCTCGG 
GCCCTGGGCC TGCTGAGTGC TGTGGAGCGG GGCCGGGCAC TGGGGGGCCG GGCTGTGCGC 
AGCCGCCGCG CCATCGCTGT GCTGTCCCGC TGGCCTGCCT TCCCGGCCTT CCGCGCCTTC 
CTCACCTTCC TCTACCGCTA CTCCGTCTCC GGCCCCCACC GTCTGCCCCT GGAAGCGCAC 
ATCTCCCACT TCATTCACAA TGTTCCCTTC CCTTCCCCAC AGAGACCCCG CATCCTGGTG 
CAGATGTCTC CCTATGACAA CCTGCTCCTC TGCCAGCCTG TATCCTCACC CCTGCCCCTC 
AGTGGTGCCA GCTTCCTGCA GCTGCTGCAG ACCCTGGGCC CGGAGCTGGC GATCACACTG 
CTGCTTGCTG TGCTCACGGA ACACAAGCTA CTAGTCCACT CGCTGCGGCC AGATCTGCTC 
ACCAGCGTCT GCGAGGCCCT TGTCTCTATG ATCTTCCCGC TGCACTGGCA GTGCCCCTAC 
ATTCCGCTGT GCCCACTGGT GCTGGCAGAT GTGCTGAGTG CCCCCGTGCC CTTCATCGTG 
GGTATCCACT CCAGCTACTT CGATCTGCAT GACCCACCTG TGGATGTCAT CTGTGTGGAT 
CTTGATACCA ACACGCTCTT CCAGACTGAG GAAAAGAAGC CCCTCTCCCC TCGGACCCTG 
CCCCGCAGAC CCTACAAGGT TCTGCTGGCC ACACTGACAC ATCTGTACCA GCAGCTGGAT 
CAGACGTATA CTGGCCCGGA GGAGGAGGCC TCCCTGGAAT TCCTGCTGAC GGACTATGAG 
GCAGTGTGTG GCCGCCGGGC CCGGCTAGAG CGCGAGGTCC AGGGAGCCTT CCTCCGCTTC 
ATGGCCTGCC TGCTCAAGGG CTACCGGGAC TTCCTGCGCC CACTCACCCA GGCCCCCTCT 
GAGGGGTCTC GCGATGTTGA CAACCTCTTC TTCCTGCAAG GCTTCCTCAA GTCCCGAGAA 
CGTTCCAGCC ACAAGCTGTA CTCCCAGCTG CTACACACAC AGATGTTCTC CCAGTTCATC 
GAGGAATGCT CCTTTGGCTC TGCTCGGCAT GCTGCCCTTG AATTCTTTGA CTCTTGTGTC 
GACAAGGTCC ACCCAGAGCA GGAGAAACCT GAGCCCGTGC CCCTGGTGGA GCTGGAGGAG 
CTGTCAGGCA GTGAGCTCAC CGTGTTTATC ATGCCTCCCG AGGAGCCGCC AGCGCCAGAG 
GGCAGTGAAT CCACTCCCCA GTACTGTTAC GATGGGTTCC CCGAGCTACG GGCTGAGCTG 
TTCGAGGCTC CTCAAGAACA AGCTGGGGCT CTGCCTGTGC CAGGCCCTTC CCGCAGCGCC 
CCCAGCAGTC CTGCCCCTCG CCGCACCAAA CAGGAGATGA AGGTGGCGCA GCGGATGGCA 
CAGAAGTCAG CAGCTGTGCC TGAGCTGTGG GCCCGGTGCC TGCTGGGCCA CTGCTACGGG 
CTGTGGTTCC TGTGTCTGCC TGCCTACGTG CGCTCGGCGC CCTCCCGGGT GCGGGCGCTG 
CAGACGGCCT ACCAGGTGCT GCGCCAGATG GAGAGCCGCA AGGTGGTGCT GCCTGACGAG 
GTGTGTTACC GGGTGCTGAT GCAGCTGTGC TCGCACTACG GGCAGCCCGT GCTGTCCGTG 
CGGGTCATGC TGGACATGCG GCGGGCGGGC ATCGTGCCCA ACACCATCAC CTATGGCTAC 
TACAACAAGG CTGTGCTGGA AAGCAAGTGG CCATCTGGCA CACCGGGTGG GCGCCTGCGC 
TGGGCCAAGC TCCGGAACGT GGTCCTGGGG GCTGCTCAGT TCCGCCAGCC CTTGCGAGAA 
CAGCGGCAGC GGCAGCAGAA GCGGGAGGAG GTGGAGACAG CAGCCCAAGA GGCAGGCAGC 
TCGAAGACAG AGCCCTATTT GGAGCGCCCC TCCCCTACCC GCCCCCTTCA GCGCCAGACT 
ACCTGGGCCG GGCGAAGCCT GCGGGACCCT GCCTCACCTA TGGGGCGCCT GGTGAAGAGT 
GGCAGCCTGG GGAGTGCCCG AGGGGCACAG CCCACCGTGG AGGCTGGTGT GGCCCACATG 
ATAGAGGCCT TGGGGGTCCT GGAACCCCGG GGATCACCCA TACCCTGGCA TGATGGAAGC 
CTCTCAGACC TGAGCCTGAC CGGGGAAGAG TCGGCACCTG GAGGCAGCCC AGGGGACTCA 
GGCTCAGCCC TGAGCACCCA GTCCACTGAA ACCCTGGAAG GGCCAAGTGG GCGGGTGCCT 
AAGGCTGGTG GGCGTCAAGA TGAGGCCAGC ACCCCCCGAA GAGGGCTGGG CGCCCGCCTC 
CAACAGCTAC TCACTCCTTC CCGCCGCTCC CCCACCTCTC GTGCCCCCCC GTCTGAGCTG 
CCCCCTGACC TGCCTCCCTC AGCCCGTCGC AGCCCCATGG ACAGCCTTCT GCACCCCCGG 
GAGCGCCCTG GATCCACTGC ATCCGAGAGC TCAGCCTCTC TGGGCAGTGA GTGGGACCTC 
TCAGAATCTT CTCTCAGCAG TGTGAGCCTT CGTCGTTCCT CAGAGCGCCT CAGTGACACC 
CCTGGATCCT TCCAGCCACC TTCCCTGGAA ATTCTGCTGT CTAGCTGCTC GCTGTGCCGC 
GCCTGTGACT CCCTGGTGTA CGATGAGGAG ATCATGGCTG GCTGGGCGCC TGACGACTCC 
AACCTCAACA CCACCTGTCC GTTCTGCGCC TGCCCCTTTG TGCCCCTGCT CAGCGTCCAG 
ACCCTTGATT CCCGACCCAG CGCCCCCAGC CCCAAGCCTG CTCCTGCTGG TGCCAGCGGC 
AGCAGCAACG CTCCTGTCCC TGGGGGCCCA GGCCCTGTGC TCAGTGACCG CAGGCTTTGC 
CTAGCCCTGG ATGAGCCGCA GCTCTGCAAC GGGCACATGG GGACTGCCTC CCGGCGTGTT 
GAGAGTGGGG CATGGGCGTA TCTCAGCCCC CTCGTGCTGC GGAAGGAGCT GGAGTCACTG 
GTAGAGAACG AGGGCAGTGA GGTGCTGGCA TTGCCTGAGT TGCCTGCCGC TCACCCCATC 
ATCTTCTGGA ACCTTCTGTG GTATTTCCAG CGGCTGCGCC TGCCCAGTAT TCTGCCATGC 
TTGGTGCTGG CCTCCCGTGA TGGGCCCCCG CCCCACCAGG CCCCAGCTCC TTGGATAATG 
CCTGATCCAG CATCCGTGCA GGTGCGGCTG CTGTGGGATG TCCTGACCCC TGATCCCGAT 
AGCTGCCCAC CTCTCTATGT GCTCTGGAGG GTCCACAGCC AGATCCCACA GCGGGTGGTA 
TGGCCAGGTC CAGTACCTGC ATCCCTTAGC CTGGCGTTGC TGGAATCGGT GCTGCGCCAC 
GTCGGTCTCA ACGAGGTGCA CAAGGCTATA GGGCTCCTGC TAGAAACTCT AGGGCCCCCT 
CCCACTGGTC TGCACCTACA AAGGGGCATC TACCGTGAGA TCTTATTCCT GACACTGGCT 
GCTCTGGGCA AGGACCACAT GGATATAGTG GCCTTCGACA AGAAGTACAA ATCCGCCTTT 
AACAAGCTGG CCAGCACCAT GGGCAAGGAG GAGCTGAGGC AGCGGCGGGC ACAGATGCCT 
ACCCCAAAGG CCATTGATTG CCGGAAATGT TTCGGAGCGC CTCTGGAATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002686064.4
CDS	147..3980
Translation	MPWASLTSAWYCPARARALLILTADCPATSTPACGAQQCTCATRWAWPRPTHWCMRQVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREEVETAAQEAGSSKTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSNAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASRDGPPPHQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASTMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_002686018.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4B (DENND4B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002686018.4

ORF Insert Sequence:

ATGCCTTGGG CATCACTGAC CTCTGCCTGG TACTGCCCAG CAAGGGCGAG GGCACTCCTC 
ATACTTACTG CCGATTGCCC CGCAACCTCA ACCCCGGCAT GTGGGGCCCA GCAGTGTACC 
TGTGCTACAA GGTGGGCCTG GCCAAGGCCA ACACACTGGT GTATGAGGCA GGTGTACGGT 
GCTGCCCTGC AGTTCTACGA GGCGTTCCCG AGGGCCAGGC TGTCGGAGCG GCAGGCTCGG 
GCCCTGGGCC TGCTGAGTGC TGTGGAGCGG GGCCGGGCAC TGGGGGGCCG GGCTGTGCGC 
AGCCGCCGCG CCATCGCTGT GCTGTCCCGC TGGCCTGCCT TCCCGGCCTT CCGCGCCTTC 
CTCACCTTCC TCTACCGCTA CTCCGTCTCC GGCCCCCACC GTCTGCCCCT GGAAGCGCAC 
ATCTCCCACT TCATTCACAA TGTTCCCTTC CCTTCCCCAC AGAGACCCCG CATCCTGGTG 
CAGATGTCTC CCTATGACAA CCTGCTCCTC TGCCAGCCTG TATCCTCACC CCTGCCCCTC 
AGTGGTGCCA GCTTCCTGCA GCTGCTGCAG ACCCTGGGCC CGGAGCTGGC GATCACACTG 
CTGCTTGCTG TGCTCACGGA ACACAAGCTA CTAGTCCACT CGCTGCGGCC AGATCTGCTC 
ACCAGCGTCT GCGAGGCCCT TGTCTCTATG ATCTTCCCGC TGCACTGGCA GTGCCCCTAC 
ATTCCGCTGT GCCCACTGGT GCTGGCAGAT GTGCTGAGTG CCCCCGTGCC CTTCATCGTG 
GGTATCCACT CCAGCTACTT CGATCTGCAT GACCCACCTG TGGATGTCAT CTGTGTGGAT 
CTTGATACCA ACACGCTCTT CCAGACTGAG GAAAAGAAGC CCCTCTCCCC TCGGACCCTG 
CCCCGCAGAC CCTACAAGGT TCTGCTGGCC ACACTGACAC ATCTGTACCA GCAGCTGGAT 
CAGACGTATA CTGGCCCGGA GGAGGAGGCC TCCCTGGAAT TCCTGCTGAC GGACTATGAG 
GCAGTGTGTG GCCGCCGGGC CCGGCTAGAG CGCGAGGTCC AGGGAGCCTT CCTCCGCTTC 
ATGGCCTGCC TGCTCAAGGG CTACCGGGAC TTCCTGCGCC CACTCACCCA GGCCCCCTCT 
GAGGGGTCTC GCGATGTTGA CAACCTCTTC TTCCTGCAAG GCTTCCTCAA GTCCCGAGAA 
CGTTCCAGCC ACAAGCTGTA CTCCCAGCTG CTACACACAC AGATGTTCTC CCAGTTCATC 
GAGGAATGCT CCTTTGGCTC TGCTCGGCAT GCTGCCCTTG AATTCTTTGA CTCTTGTGTC 
GACAAGGTCC ACCCAGAGCA GGAGAAACCT GAGCCCGTGC CCCTGGTGGA GCTGGAGGAG 
CTGTCAGGCA GTGAGCTCAC CGTGTTTATC ATGCCTCCCG AGGAGCCGCC AGCGCCAGAG 
GGCAGTGAAT CCACTCCCCA GTACTGTTAC GATGGGTTCC CCGAGCTACG GGCTGAGCTG 
TTCGAGGCTC CTCAAGAACA AGCTGGGGCT CTGCCTGTGC CAGGCCCTTC CCGCAGCGCC 
CCCAGCAGTC CTGCCCCTCG CCGCACCAAA CAGGAGATGA AGGTGGCGCA GCGGATGGCA 
CAGAAGTCAG CAGCTGTGCC TGAGCTGTGG GCCCGGTGCC TGCTGGGCCA CTGCTACGGG 
CTGTGGTTCC TGTGTCTGCC TGCCTACGTG CGCTCGGCGC CCTCCCGGGT GCGGGCGCTG 
CAGACGGCCT ACCAGGTGCT GCGCCAGATG GAGAGCCGCA AGGTGGTGCT GCCTGACGAG 
GTGTGTTACC GGGTGCTGAT GCAGCTGTGC TCGCACTACG GGCAGCCCGT GCTGTCCGTG 
CGGGTCATGC TGGACATGCG GCGGGCGGGC ATCGTGCCCA ACACCATCAC CTATGGCTAC 
TACAACAAGG CTGTGCTGGA AAGCAAGTGG CCATCTGGCA CACCGGGTGG GCGCCTGCGC 
TGGGCCAAGC TCCGGAACGT GGTCCTGGGG GCTGCTCAGT TCCGCCAGCC CTTGCGAGAA 
CAGCGGCAGC GGCAGCAGAA GCGGGAGGAG GTGGAGACAG CAGCCCAAGA GGCAGGCAGC 
TCGAAGACAG AGCCCTATTT GGAGCGCCCC TCCCCTACCC GCCCCCTTCA GCGCCAGACT 
ACCTGGGCCG GGCGAAGCCT GCGGGACCCT GCCTCACCTA TGGGGCGCCT GGTGAAGAGT 
GGCAGCCTGG GGAGTGCCCG AGGGGCACAG CCCACCGTGG AGGCTGGTGT GGCCCACATG 
ATAGAGGCCT TGGGGGTCCT GGAACCCCGG GGATCACCCA TACCCTGGCA TGATGGAAGC 
CTCTCAGACC TGAGCCTGAC CGGGGAAGAG TCGGCACCTG GAGGCAGCCC AGGGGACTCA 
GGCTCAGCCC TGAGCACCCA GTCCACTGAA ACCCTGGAAG GGCCAAGTGG GCGGGTGCCT 
AAGGCTGGTG GGCGTCAAGA TGAGGCCAGC ACCCCCCGAA GAGGGCTGGG CGCCCGCCTC 
CAACAGCTAC TCACTCCTTC CCGCCGCTCC CCCACCTCTC GTGCCCCCCC GTCTGAGCTG 
CCCCCTGACC TGCCTCCCTC AGCCCGTCGC AGCCCCATGG ACAGCCTTCT GCACCCCCGG 
GAGCGCCCTG GATCCACTGC ATCCGAGAGC TCAGCCTCTC TGGGCAGTGA GTGGGACCTC 
TCAGAATCTT CTCTCAGCAG TGTGAGCCTT CGTCGTTCCT CAGAGCGCCT CAGTGACACC 
CCTGGATCCT TCCAGCCACC TTCCCTGGAA ATTCTGCTGT CTAGCTGCTC GCTGTGCCGC 
GCCTGTGACT CCCTGGTGTA CGATGAGGAG ATCATGGCTG GCTGGGCGCC TGACGACTCC 
AACCTCAACA CCACCTGTCC GTTCTGCGCC TGCCCCTTTG TGCCCCTGCT CAGCGTCCAG 
ACCCTTGATT CCCGACCCAG CGCCCCCAGC CCCAAGCCTG CTCCTGCTGG TGCCAGCGGC 
AGCAGCAACG CTCCTGTCCC TGGGGGCCCA GGCCCTGTGC TCAGTGACCG CAGGCTTTGC 
CTAGCCCTGG ATGAGCCGCA GCTCTGCAAC GGGCACATGG GGACTGCCTC CCGGCGTGTT 
GAGAGTGGGG CATGGGCGTA TCTCAGCCCC CTCGTGCTGC GGAAGGAGCT GGAGTCACTG 
GTAGAGAACG AGGGCAGTGA GGTGCTGGCA TTGCCTGAGT TGCCTGCCGC TCACCCCATC 
ATCTTCTGGA ACCTTCTGTG GTATTTCCAG CGGCTGCGCC TGCCCAGTAT TCTGCCATGC 
TTGGTGCTGG CCTCCCGTGA TGGGCCCCCG CCCCACCAGG CCCCAGCTCC TTGGATAATG 
CCTGATCCAG CATCCGTGCA GGTGCGGCTG CTGTGGGATG TCCTGACCCC TGATCCCGAT 
AGCTGCCCAC CTCTCTATGT GCTCTGGAGG GTCCACAGCC AGATCCCACA GCGGGTGGTA 
TGGCCAGGTC CAGTACCTGC ATCCCTTAGC CTGGCGTTGC TGGAATCGGT GCTGCGCCAC 
GTCGGTCTCA ACGAGGTGCA CAAGGCTATA GGGCTCCTGC TAGAAACTCT AGGGCCCCCT 
CCCACTGGTC TGCACCTACA AAGGGGCATC TACCGTGAGA TCTTATTCCT GACACTGGCT 
GCTCTGGGCA AGGACCACAT GGATATAGTG GCCTTCGACA AGAAGTACAA ATCCGCCTTT 
AACAAGCTGG CCAGCACCAT GGGCAAGGAG GAGCTGAGGC AGCGGCGGGC ACAGATGCCT 
ACCCCAAAGG CCATTGATTG CCGGAAATGT TTCGGAGCGC CTCTGGAATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_024989951.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845719.1 Bos taurus DENN domain containing 4B (DENND4B), transcript variant X1, mRNA.

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CloneID	OBa233176
Clone ID Related Accession (Same CDS sequence)	XM_024989951.1
Accession Version	XM_024989951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DENN domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACG 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC CCTGGGCGAG GAGGTGCCCC AGGGCTATAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG TCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTATAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCTTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCCGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCGGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CCGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACAC TGCTGCTTGC TGTGCTCACG GAACACAAGC TACTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACGCTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCG GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTG TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCCTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCAGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCAG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ACGATGGGTT CCCCGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCTGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCGC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCGCACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCATCTGG CACACCGGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGG AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGAAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCCCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CTGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGCAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC TGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CTAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCGTCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGTGTGAGCC TTCGTCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TACGATGAGG AGATCATGGC TGGCTGGGCG 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCAA CGCTCCTGTC CCTGGGGGCC CAGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCG CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCAT GCTTGGTGCT GGCCTCCCGT GATGGGCCCC CGCCCCACCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCCG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG CCAGATCCCA 
CAGCGGGTGG TATGGCCAGG TCCAGTACCT GCATCCCTTA GCCTGGCGTT GCTGGAATCG 
GTGCTGCGCC ACGTCGGTCT CAACGAGGTG CACAAGGCTA TAGGGCTCCT GCTAGAAACT 
CTAGGGCCCC CTCCCACTGG TCTGCACCTA CAAAGGGGCA TCTACCGTGA GATCTTATTC 
CTGACACTGG CTGCTCTGGG CAAGGACCAC ATGGATATAG TGGCCTTCGA CAAGAAGTAC 
AAATCCGCCT TTAACAAGCT GGCCAGCACC ATGGGCAAGG AGGAGCTGAG GCAGCGGCGG 
GCACAGATGC CTACCCCAAA GGCCATTGAT TGCCGGAAAT GTTTCGGAGC GCCTCTGGAA 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845719.1
CDS	1010..5515
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWIPEPSGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQTLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPVDVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTHLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLRPLTQAPSEGSRDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPVPLVELEELSGSELTVFIMPPEEPPAPEGSESTPQYCYDGFPELRAELFEAPQEQAGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALQTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLDMRRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLREQRQRQQKREEVETAAQEAGSSKTEPYLERPSPTRPLQRQTTWAGRSLRDPASPMGRLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPIPWHDGSLSDLSLTGEESAPGGSPGDSGSALSTQSTETLEGPSGRVPKAGGRQDEASTPRRGLGARLQQLLTPSRRSPTSRAPPSELPPDLPPSARRSPMDSLLHPRERPGSTASESSASLGSEWDLSESSLSSVSLRRSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKPAPAGASGSSNAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGTASRRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPAAHPIIFWNLLWYFQRLRLPSILPCLVLASRDGPPPHQAPAPWIMPDPASVQVRLLWDVLTPDPDSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAIGLLLETLGPPPTGLHLQRGIYREILFLTLAALGKDHMDIVAFDKKYKSAFNKLASTMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_024989951.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DENN domain containing 4B (DENND4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989951.1

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTG 
GTGGATTACT TCGTGATAGC TGGACTTGCA GGGAACGGAG CACCCATCCC TGAGGAAACG 
TGGATTCCTG AACCCAGTGG ACCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC CCTGGGCGAG GAGGTGCCCC AGGGCTATAC ATGCATCCAG 
GCTTCCGCGG GGGGCCACCC CTTGGAACTC AGCGCTGGGC TCCTAGGTGG AACTCAACCT 
GTCATCTGCT ACCGGAGGGG TCGTGACAAG CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ATACGACACC CTATAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGCCCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAGG GGGCAGGGCT GCATGCCTTG GGCATCACTG ACCTCTGCCT GGTACTGCCC 
AGCAAGGGCG AGGGCACTCC TCATACTTAC TGCCGATTGC CCCGCAACCT CAACCCCGGC 
ATGTGGGGCC CAGCAGTGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACACTG 
GTGTATGAGG CAGAGCTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCAG TGCCCGTCTT CTGCCTGCCC ATGGGGGCCA CTATCGAGTG CTGGCCTGCC 
CAGACCAAGT ACCCCGTGCC CGTCTTCTCC ACCTTTGTGC TCACGGGTGC AGCTGGTGAT 
AAGGTGTACG GTGCTGCCCT GCAGTTCTAC GAGGCGTTCC CGAGGGCCAG GCTGTCGGAG 
CGGCAGGCTC GGGCCCTGGG CCTGCTGAGT GCTGTGGAGC GGGGCCGGGC ACTGGGGGGC 
CGGGCTGTGC GCAGCCGCCG CGCCATCGCT GTGCTGTCCC GCTGGCCTGC CTTCCCGGCC 
TTCCGCGCCT TCCTCACCTT CCTCTACCGC TACTCCGTCT CCGGCCCCCA CCGTCTGCCC 
CTGGAAGCGC ACATCTCCCA CTTCATTCAC AATGTTCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC TCCCTATGAC AACCTGCTCC TCTGCCAGCC TGTATCCTCA 
CCCCTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGACCCTGGG CCCGGAGCTG 
GCGATCACAC TGCTGCTTGC TGTGCTCACG GAACACAAGC TACTAGTCCA CTCGCTGCGG 
CCAGATCTGC TCACCAGCGT CTGCGAGGCC CTTGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCGCT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG TGCCCCCGTG 
CCCTTCATCG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCACC TGTGGATGTC 
ATCTGTGTGG ATCTTGATAC CAACACGCTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACCC TGCCCCGCAG ACCCTACAAG GTTCTGCTGG CCACACTGAC ACATCTGTAC 
CAGCAGCTGG ATCAGACGTA TACTGGCCCG GAGGAGGAGG CCTCCCTGGA ATTCCTGCTG 
ACGGACTATG AGGCAGTGTG TGGCCGCCGG GCCCGGCTAG AGCGCGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG CCTGCTCAAG GGCTACCGGG ACTTCCTGCG CCCACTCACC 
CAGGCCCCCT CTGAGGGGTC TCGCGATGTT GACAACCTCT TCTTCCTGCA AGGCTTCCTC 
AAGTCCCGAG AACGTTCCAG CCACAAGCTG TACTCCCAGC TGCTACACAC ACAGATGTTC 
TCCCAGTTCA TCGAGGAATG CTCCTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TCGACAAGGT CCACCCAGAG CAGGAGAAAC CTGAGCCCGT GCCCCTGGTG 
GAGCTGGAGG AGCTGTCAGG CAGTGAGCTC ACCGTGTTTA TCATGCCTCC CGAGGAGCCG 
CCAGCGCCAG AGGGCAGTGA ATCCACTCCC CAGTACTGTT ACGATGGGTT CCCCGAGCTA 
CGGGCTGAGC TGTTCGAGGC TCCTCAAGAA CAAGCTGGGG CTCTGCCTGT GCCAGGCCCT 
TCCCGCAGCG CCCCCAGCAG TCCTGCCCCT CGCCGCACCA AACAGGAGAT GAAGGTGGCG 
CAGCGGATGG CACAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTGCTGGGC 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCCTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCGC TGCAGACGGC CTACCAGGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCTGACG AGGTGTGTTA CCGGGTGCTG ATGCAGCTGT GCTCGCACTA CGGGCAGCCC 
GTGCTGTCCG TGCGGGTCAT GCTGGACATG CGGCGGGCGG GCATCGTGCC CAACACCATC 
ACCTATGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCATCTGG CACACCGGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTGGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGCGAG AACAGCGGCA GCGGCAGCAG AAGCGGGAGG AGGTGGAGAC AGCAGCCCAA 
GAGGCAGGCA GCTCGAAGAC AGAGCCCTAT TTGGAGCGCC CCTCCCCTAC CCGCCCCCTT 
CAGCGCCAGA CTACCTGGGC CGGGCGAAGC CTGCGGGACC CTGCCTCACC TATGGGGCGC 
CTGGTGAAGA GTGGCAGCCT GGGGAGTGCC CGAGGGGCAC AGCCCACCGT GGAGGCTGGT 
GTGGCCCACA TGATAGAGGC CTTGGGGGTC CTGGAACCCC GGGGATCACC CATACCCTGG 
CATGATGGAA GCCTCTCAGA CCTGAGCCTG ACCGGGGAAG AGTCGGCACC TGGAGGCAGC 
CCAGGGGACT CAGGCTCAGC CCTGAGCACC CAGTCCACTG AAACCCTGGA AGGGCCAAGT 
GGGCGGGTGC CTAAGGCTGG TGGGCGTCAA GATGAGGCCA GCACCCCCCG AAGAGGGCTG 
GGCGCCCGCC TCCAACAGCT ACTCACTCCT TCCCGCCGCT CCCCCACCTC TCGTGCCCCC 
CCGTCTGAGC TGCCCCCTGA CCTGCCTCCC TCAGCCCGTC GCAGCCCCAT GGACAGCCTT 
CTGCACCCCC GGGAGCGCCC TGGATCCACT GCATCCGAGA GCTCAGCCTC TCTGGGCAGT 
GAGTGGGACC TCTCAGAATC TTCTCTCAGC AGTGTGAGCC TTCGTCGTTC CTCAGAGCGC 
CTCAGTGACA CCCCTGGATC CTTCCAGCCA CCTTCCCTGG AAATTCTGCT GTCTAGCTGC 
TCGCTGTGCC GCGCCTGTGA CTCCCTGGTG TACGATGAGG AGATCATGGC TGGCTGGGCG 
CCTGACGACT CCAACCTCAA CACCACCTGT CCGTTCTGCG CCTGCCCCTT TGTGCCCCTG 
CTCAGCGTCC AGACCCTTGA TTCCCGACCC AGCGCCCCCA GCCCCAAGCC TGCTCCTGCT 
GGTGCCAGCG GCAGCAGCAA CGCTCCTGTC CCTGGGGGCC CAGGCCCTGT GCTCAGTGAC 
CGCAGGCTTT GCCTAGCCCT GGATGAGCCG CAGCTCTGCA ACGGGCACAT GGGGACTGCC 
TCCCGGCGTG TTGAGAGTGG GGCATGGGCG TATCTCAGCC CCCTCGTGCT GCGGAAGGAG 
CTGGAGTCAC TGGTAGAGAA CGAGGGCAGT GAGGTGCTGG CATTGCCTGA GTTGCCTGCC 
GCTCACCCCA TCATCTTCTG GAACCTTCTG TGGTATTTCC AGCGGCTGCG CCTGCCCAGT 
ATTCTGCCAT GCTTGGTGCT GGCCTCCCGT GATGGGCCCC CGCCCCACCA GGCCCCAGCT 
CCTTGGATAA TGCCTGATCC AGCATCCGTG CAGGTGCGGC TGCTGTGGGA TGTCCTGACC 
CCTGATCCCG ATAGCTGCCC ACCTCTCTAT GTGCTCTGGA GGGTCCACAG CCAGATCCCA 
CAGCGGGTGG TATGGCCAGG TCCAGTACCT GCATCCCTTA GCCTGGCGTT GCTGGAATCG 
GTGCTGCGCC ACGTCGGTCT CAACGAGGTG CACAAGGCTA TAGGGCTCCT GCTAGAAACT 
CTAGGGCCCC CTCCCACTGG TCTGCACCTA CAAAGGGGCA TCTACCGTGA GATCTTATTC 
CTGACACTGG CTGCTCTGGG CAAGGACCAC ATGGATATAG TGGCCTTCGA CAAGAAGTAC 
AAATCCGCCT TTAACAAGCT GGCCAGCACC ATGGGCAAGG AGGAGCTGAG GCAGCGGCGG 
GCACAGATGC CTACCCCAAA GGCCATTGAT TGCCGGAAAT GTTTCGGAGC GCCTCTGGAA 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DENND4B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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