PTCH2 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_584648.8	XP_584648.3	protein patched homolog 2 isoform X2
XM_005197905.3	XP_005197962.1	protein patched homolog 2 isoform X3
XM_010803749.3	XP_010802051.2	protein patched homolog 2 isoform X5
XM_010803747.2	XP_010802049.1	protein patched homolog 2 isoform X4
XM_010803749.2	XP_010802051.1	protein patched homolog 2 isoform X5
XM_010827055.2	XP_010825357.1	protein patched homolog 2 isoform X5
XM_010803747.3	XP_010802049.2	protein patched homolog 2 isoform X4
XM_005204892.3	XP_005204949.1	protein patched homolog 2 isoform X1
XM_002686431.5	XP_002686477.1	protein patched homolog 2 isoform X2
XM_005204893.3	XP_005204950.1	protein patched homolog 2 isoform X3
XM_005197904.3	XP_005197961.1	protein patched homolog 2 isoform X1
XM_005204893.4	XP_005204950.2	protein patched homolog 2 isoform X3
XM_010827054.2	XP_010825356.1	protein patched homolog 2 isoform X4
XM_005204892.4	XP_005204949.2	protein patched homolog 2 isoform X1
XM_002686431.6	XP_002686477.2	protein patched homolog 2 isoform X2
XM_024990175.1	XP_024845943.1	protein patched homolog 2 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa233340	XM_024990175.1 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa233335	XM_005204892.4 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa233336	XM_002686431.6 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa233337	XM_005204893.4 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa233338	XM_010803747.3 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa233339	XM_010803749.3 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa03591	XM_005197904.3 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa03591	XM_005204892.3 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa03592	XM_584648.8 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa03592	XM_002686431.5 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa03593	XM_005197905.3 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa03593	XM_005204893.3 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa47010	XM_010827054.2 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa47010	XM_010803747.2 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa47011	XM_010803749.2 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa47011	XM_010827055.2 Latest version!	Bos taurus patched 2 (PTCH2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

PTCH2 ( XM_024990175.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845943.1 Bos taurus patched 2 (PTCH2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa233340
Clone ID Related Accession (Same CDS sequence)	XM_024990175.1
Accession Version	XM_024990175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCAGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATTGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGCGCT 
TCAGTTCCCT CAAGGCCGTG CTGCCCCCAC CGGCCACCCA GGCACCCCGC ACCTGGCTGC 
ACTACTACCG CAGCTGGCTA CAGGGAATCC AGGCTGCATT TGACCAGGAC TGGGCTTCTG 
GGCGCATCAC CCGCCACTCA TACCGCAATG GCTCTGAGGA TGGGGCCCTG GCATACAAGC 
TGCTCGTCCA GACCGGGGAT GCCCAGGAGC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845943.1
CDS	399..2834
Translation	MARPPPLGELPPGYTPPARPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLSASVPSRPCCPHRPPRHPAPGCTTTAAGYRESRLHLTRTGLLGASPATHTAMALRMGPWHTSCSSRPGMPRSL

Target ORF information:

RefSeq Version	XM_024990175.1
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990175.1

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCAGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATTGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGCGCT 
TCAGTTCCCT CAAGGCCGTG CTGCCCCCAC CGGCCACCCA GGCACCCCGC ACCTGGCTGC 
ACTACTACCG CAGCTGGCTA CAGGGAATCC AGGCTGCATT TGACCAGGAC TGGGCTTCTG 
GGCGCATCAC CCGCCACTCA TACCGCAATG GCTCTGAGGA TGGGGCCCTG GCATACAAGC 
TGCTCGTCCA GACCGGGGAT GCCCAGGAGC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_005204892.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204949.2 Bos taurus patched 2 (PTCH2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa233335
Clone ID Related Accession (Same CDS sequence)	XM_005204892.4
Accession Version	XM_005204892.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204892.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCAGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATTGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGGCTT CCTGACCGCT CAGGGTAGCC GGAACCTGCG GGCTGCCCGG 
GCCCTGGAGC GCACATTTGC TCCAGTGACC GATGGGGCCA TCTCCACGTT GCTAGGTCTG 
CTCATGCTTG CTGGCTCCAA CTTTGACTTC ATCGTAAGGT ACTTCTTTGT GGTGCTGACA 
ATACTTACAC TCCTGGGCCT CCTCCATGGG CTCGTGCTGC TGCCGGTACT GCTGTCCATC 
CTGGGCCCCC CACCAGAGGT GGTACAGATG TACAAGGAGA GCACAGAGGT CCTGAGCCCC 
CCAGCTCCGC AGGGAGGAGG GCTCAGGTGG GGGGTACCCT CCACCCTGCC CCAGAGCTTT 
GCCAGAGTGA CTACCTCCAT GACTGTGGCC CTCCACCCAC CCCCACTGCC TGGTGCCTAC 
ATCCACCCAG CCTCTGAGGA GCCTACTTGG TCGCCTGCTG CCACACCAGC TGCCAATGGC 
CCCAGCAACC TCGGGCCTAG GGGACTGTGT CCAGCCACGG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204949.2
CDS	364..4008
Translation	MARPPPLGELPPGYTPPARPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTAQGSRNLRAARALERTFAPVTDGAISTLLGLLMLAGSNFDFIVRYFFVVLTILTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESTEVLSPPAPQGGGLRWGVPSTLPQSFARVTTSMTVALHPPPLPGAYIHPASEEPTWSPAATPAANGPSNLGPRGLCPATG

Target ORF information:

RefSeq Version	XM_005204892.4
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204892.4

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCAGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATTGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGGCTT CCTGACCGCT CAGGGTAGCC GGAACCTGCG GGCTGCCCGG 
GCCCTGGAGC GCACATTTGC TCCAGTGACC GATGGGGCCA TCTCCACGTT GCTAGGTCTG 
CTCATGCTTG CTGGCTCCAA CTTTGACTTC ATCGTAAGGT ACTTCTTTGT GGTGCTGACA 
ATACTTACAC TCCTGGGCCT CCTCCATGGG CTCGTGCTGC TGCCGGTACT GCTGTCCATC 
CTGGGCCCCC CACCAGAGGT GGTACAGATG TACAAGGAGA GCACAGAGGT CCTGAGCCCC 
CCAGCTCCGC AGGGAGGAGG GCTCAGGTGG GGGGTACCCT CCACCCTGCC CCAGAGCTTT 
GCCAGAGTGA CTACCTCCAT GACTGTGGCC CTCCACCCAC CCCCACTGCC TGGTGCCTAC 
ATCCACCCAG CCTCTGAGGA GCCTACTTGG TCGCCTGCTG CCACACCAGC TGCCAATGGC 
CCCAGCAACC TCGGGCCTAG GGGACTGTGT CCAGCCACGG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_002686431.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002686477.2 Bos taurus patched 2 (PTCH2), transcript variant X2, mRNA.

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CloneID	OBa233336
Clone ID Related Accession (Same CDS sequence)	XM_002686431.6
Accession Version	XM_002686431.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002686431.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCAGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATTGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CACAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002686477.2
CDS	355..3978
Translation	MARPPPLGELPPGYTPPARPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTAQGSRNLRAARALERTFAPVTDGAISTLLGLLMLAGSNFDFIVRYFFVVLTILTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESTEVLSPPAPQGGGLRWGVPSTLPQSFARVTTSMTVALHPPPLPGAYIHPASEEPTWSPAATPAANGPSNLGPRGLCPATG

Target ORF information:

RefSeq Version	XM_002686431.6
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002686431.6

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCAGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATTGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CACAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_005204893.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204950.2 Bos taurus patched 2 (PTCH2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa233337
Clone ID Related Accession (Same CDS sequence)	XM_005204893.4
Accession Version	XM_005204893.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204893.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGTCCTG GGATCTGAAC AAAATCTGCT ACAAGTCAGG AGTTCCCCTA 
ATTGAAAATG GAATGATTGA GCGGATGATT GAGAAGCTGT TTCCCTGCGT GATCCTCACC 
CCCCTCGACT GCTTCTGGGA GGGAGCCAAA CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC 
CGCCCAGACA TCCAGTGGAC CAACCTGGAC CCAGAGCAGC TGCTGGCGGA GCTGGGCCCC 
TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAC 
GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CACCCAGTGC CCCTAACCAT 
CACAGCAGGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC 
CACAAGTTTA TGCACTGGCA GGAGGAGCTG CTGCTGGGAG GCATGGCCAG AGACCCCCAA 
GGACAGCTGC TGAGGGCAGA GGCCCTGCAG AGCACCTTCC TGCTAATGAG CCCCCGCCAG 
CTCTACGAGC ACTTCCGAGG TGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG 
GCCGGCACGG TCCTTCAGGC CTGGCAGCGA CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG 
CCCGAGAACG CATCCCAGCA GATCCACGCC TTCTCCTCTA CCACCCTGGA CGACATCCTG 
CACGCCTTCT CTGAAGTCAG CGCTGCCCGT GTGGTGGGGG GCTACCTGCT CATGCTAGCC 
TATGCCTGTG TGACAATGCT GCGGTGGGAC TGTGCCCAGT CCCAGGGTGC AGTGGGCCTC 
GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG GCCTCAGGCC TGGGGCTCTG TGCCCTGTTG 
GGCATTGCCT TCAACGCTGC CACTACCCAG GTGCTGCCTT TCTTGGCACT GGGCATCGGC 
GTGGACGACA TATTCCTGCT GGCACACGCC TTCACAGAGG CTCCACCTGG CAGCCCTCTC 
CAGGAGCGCA CGGGGGAGTG TCTGCGACGC ACAGGCACCA GTGTCACACT CACATCCATC 
AACAATATGG TTGCCTTCTT CATGGCCGCC CTAGTTCCCA TCCCTGCACT GCGGGCCTTC 
TCCTTGCAGG CAGCCATAGT GGTGGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA 
GCGGTCCTCA GCCTGGACCT GCGGCGGCGC CACTGCCGGC GCCTGGATGT GCTCTGCTGC 
TTCTCTAGCC CCTGCTCTGC TCGGGTGATT CAGATTCTGC CCCAGGAGCT GGGGAATGGG 
ACGGTACCAG TGGGTGTTGC CCACCTGACT GCCACGGTTC AAGCCTTTGC CCACTGTGAA 
GCCAGCAGCC AACATGTGGT CACCATCTTG CCTCCCCAAG CCCAGCTGGT GCCGCCACCT 
TCTGACCCTT TGGGCTCTGA GCTCTTCAGC CCAGGAGGGT CCACACGGGA CCTTCTAGGG 
CAGGAGGAGG GGACAGGGCA GAAGGCAGCC TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT 
CTTGCCCATT TCGCCCGCTC TCAGTTTGCA CCCTTGTTGC TCCAGTCCCA CACCAAGCTT 
AGTCGCCTTT CCCCACAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CACAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204950.2
CDS	373..3996
Translation	MARPPPLGELPPGYTPPARPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTAQGSRNLRAARALERTFAPVTDGAISTLLGLLMLAGSNFDFIVRYFFVVLTILTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESTEVLSPPAPQGGGLRWGVPSTLPQSFARVTTSMTVALHPPPLPGAYIHPASEEPTWSPAATPAANGPSNLGPRGLCPATG

Target ORF information:

RefSeq Version	XM_005204893.4
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204893.4

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGTCCTG GGATCTGAAC AAAATCTGCT ACAAGTCAGG AGTTCCCCTA 
ATTGAAAATG GAATGATTGA GCGGATGATT GAGAAGCTGT TTCCCTGCGT GATCCTCACC 
CCCCTCGACT GCTTCTGGGA GGGAGCCAAA CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC 
CGCCCAGACA TCCAGTGGAC CAACCTGGAC CCAGAGCAGC TGCTGGCGGA GCTGGGCCCC 
TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAC 
GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CACCCAGTGC CCCTAACCAT 
CACAGCAGGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC 
CACAAGTTTA TGCACTGGCA GGAGGAGCTG CTGCTGGGAG GCATGGCCAG AGACCCCCAA 
GGACAGCTGC TGAGGGCAGA GGCCCTGCAG AGCACCTTCC TGCTAATGAG CCCCCGCCAG 
CTCTACGAGC ACTTCCGAGG TGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG 
GCCGGCACGG TCCTTCAGGC CTGGCAGCGA CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG 
CCCGAGAACG CATCCCAGCA GATCCACGCC TTCTCCTCTA CCACCCTGGA CGACATCCTG 
CACGCCTTCT CTGAAGTCAG CGCTGCCCGT GTGGTGGGGG GCTACCTGCT CATGCTAGCC 
TATGCCTGTG TGACAATGCT GCGGTGGGAC TGTGCCCAGT CCCAGGGTGC AGTGGGCCTC 
GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG GCCTCAGGCC TGGGGCTCTG TGCCCTGTTG 
GGCATTGCCT TCAACGCTGC CACTACCCAG GTGCTGCCTT TCTTGGCACT GGGCATCGGC 
GTGGACGACA TATTCCTGCT GGCACACGCC TTCACAGAGG CTCCACCTGG CAGCCCTCTC 
CAGGAGCGCA CGGGGGAGTG TCTGCGACGC ACAGGCACCA GTGTCACACT CACATCCATC 
AACAATATGG TTGCCTTCTT CATGGCCGCC CTAGTTCCCA TCCCTGCACT GCGGGCCTTC 
TCCTTGCAGG CAGCCATAGT GGTGGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA 
GCGGTCCTCA GCCTGGACCT GCGGCGGCGC CACTGCCGGC GCCTGGATGT GCTCTGCTGC 
TTCTCTAGCC CCTGCTCTGC TCGGGTGATT CAGATTCTGC CCCAGGAGCT GGGGAATGGG 
ACGGTACCAG TGGGTGTTGC CCACCTGACT GCCACGGTTC AAGCCTTTGC CCACTGTGAA 
GCCAGCAGCC AACATGTGGT CACCATCTTG CCTCCCCAAG CCCAGCTGGT GCCGCCACCT 
TCTGACCCTT TGGGCTCTGA GCTCTTCAGC CCAGGAGGGT CCACACGGGA CCTTCTAGGG 
CAGGAGGAGG GGACAGGGCA GAAGGCAGCC TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT 
CTTGCCCATT TCGCCCGCTC TCAGTTTGCA CCCTTGTTGC TCCAGTCCCA CACCAAGCTT 
AGTCGCCTTT CCCCACAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CACAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_010803747.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802049.2 Bos taurus patched 2 (PTCH2), transcript variant X4, mRNA.

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CloneID	OBa233338
Clone ID Related Accession (Same CDS sequence)	XM_010803747.3
Accession Version	XM_010803747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCAGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATTGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGAC 
CACACCATCC TGCTCCCAGC CCACGGGGCG GGGTGGGATA GAACCAAAAC AAAACACCCT 
CAGTGGCCAA CAGACAGGGC TCAACTCACA GGACCCCTTC CCCATGAAGT ACTACCAGCT 
GAAGTTAGCA GCCTCCCCTT TTGCCCAGAC ATCATGTCCC TGCCCTCCAG CCCTCCTAAC 
TTCTTCTTGA GACCCACCTT AGCCTTTAGG TGCAGGATTG GCCCCTGGTC TCAACATCCT 
GTCCCTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802049.2
CDS	407..3898
Translation	MARPPPLGELPPGYTPPARPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALVLLCGADNTYTPGPPPWARAAAGTAVHPGPPTRGDHTILLPAHGAGWDRTKTKHPQWPTDRAQLTGPLPHEVLPAEVSSLPFCPDIMSLPSSPPNFFLRPTLAFRCRIGPWSQHPVPC

Target ORF information:

RefSeq Version	XM_010803747.3
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803747.3

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCAGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATTGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGAC 
CACACCATCC TGCTCCCAGC CCACGGGGCG GGGTGGGATA GAACCAAAAC AAAACACCCT 
CAGTGGCCAA CAGACAGGGC TCAACTCACA GGACCCCTTC CCCATGAAGT ACTACCAGCT 
GAAGTTAGCA GCCTCCCCTT TTGCCCAGAC ATCATGTCCC TGCCCTCCAG CCCTCCTAAC 
TTCTTCTTGA GACCCACCTT AGCCTTTAGG TGCAGGATTG GCCCCTGGTC TCAACATCCT 
GTCCCTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_010803749.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802051.2 Bos taurus patched 2 (PTCH2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa233339
Clone ID Related Accession (Same CDS sequence)	XM_010803749.3
Accession Version	XM_010803749.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803749.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCAGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATTGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGGT 
ACAGATGTAC AAGGAGAGCA CAGAGGTCCT GAGCCCCCCA GCTCCGCAGG GAGGAGGGCT 
CAGGTGGGGG GTACCCTCCA CCCTGCCCCA GAGCTTTGCC AGAGTGACTA CCTCCATGAC 
TGTGGCCCTC CACCCACCCC CACTGCCTGG TGCCTACATC CACCCAGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802051.2
CDS	391..3804
Translation	MARPPPLGELPPGYTPPARPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALVLLCGADNTYTPGPPPWARAAAGTAVHPGPPTRGGTDVQGEHRGPEPPSSAGRRAQVGGTLHPAPELCQSDYLHDCGPPPTPTAWCLHPPSL

Target ORF information:

RefSeq Version	XM_010803749.3
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803749.3

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGCTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCGG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCAGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATTGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGGT 
ACAGATGTAC AAGGAGAGCA CAGAGGTCCT GAGCCCCCCA GCTCCGCAGG GAGGAGGGCT 
CAGGTGGGGG GTACCCTCCA CCCTGCCCCA GAGCTTTGCC AGAGTGACTA CCTCCATGAC 
TGTGGCCCTC CACCCACCCC CACTGCCTGG TGCCTACATC CACCCAGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_005204892.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204949.1 Bos taurus patched 2 (PTCH2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa03591
Clone ID Related Accession (Same CDS sequence)	XM_005204892.3 , XM_005197904.3
Accession Version	XM_005204892.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204892.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGGCTT CCTGACCGCT CAGGGTAGCC GGAACCTGCG GGCTGCCCGG 
GCCCTGGAGC GCACATTTGC TCCAGTGACC GATGGGGCCA TCTCCACGTT GCTAGGTCTG 
CTCATGCTTG CTGGCTCCAA CTTTGACTTC ATCGTAAGGT ACTTCTTTGT GGTGCTGACA 
ATACTTACAC TCCTGGGCCT CCTCCATGGG CTCGTGCTGC TGCCGGTACT GCTGTCCATC 
CTGGGCCCCC CACCAGAGGT GGTACAGATG TACAAGGAGA GCGCAGAGGT CCTGAGCCCC 
CCAGCTCCGC AGGGAGGAGG GCTCAGGTGG GGGGTACCCT CCACCCTGCC CCAGAGCTTT 
GCCAGAGTGA CTACCTCCAT GACTGTGGCC CTCCACCCAC CCCCACTGCC TGGTGCCTAC 
ATCCACCCAG CCTCTGAGGA GCCTACTTGG TCGCCTGCTG CCACACCAGC TGCCAATGGC 
CCCAGCAACC TCGGGCCTAG GGGACTGTGT CCAGCCACGG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204949.1
CDS	332..3976
Translation	MARPPPLGELPPGYTPPGRPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTAQGSRNLRAARALERTFAPVTDGAISTLLGLLMLAGSNFDFIVRYFFVVLTILTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESAEVLSPPAPQGGGLRWGVPSTLPQSFARVTTSMTVALHPPPLPGAYIHPASEEPTWSPAATPAANGPSNLGPRGLCPATG

Target ORF information:

RefSeq Version	XM_005204892.3
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204892.3

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGGCTT CCTGACCGCT CAGGGTAGCC GGAACCTGCG GGCTGCCCGG 
GCCCTGGAGC GCACATTTGC TCCAGTGACC GATGGGGCCA TCTCCACGTT GCTAGGTCTG 
CTCATGCTTG CTGGCTCCAA CTTTGACTTC ATCGTAAGGT ACTTCTTTGT GGTGCTGACA 
ATACTTACAC TCCTGGGCCT CCTCCATGGG CTCGTGCTGC TGCCGGTACT GCTGTCCATC 
CTGGGCCCCC CACCAGAGGT GGTACAGATG TACAAGGAGA GCGCAGAGGT CCTGAGCCCC 
CCAGCTCCGC AGGGAGGAGG GCTCAGGTGG GGGGTACCCT CCACCCTGCC CCAGAGCTTT 
GCCAGAGTGA CTACCTCCAT GACTGTGGCC CTCCACCCAC CCCCACTGCC TGGTGCCTAC 
ATCCACCCAG CCTCTGAGGA GCCTACTTGG TCGCCTGCTG CCACACCAGC TGCCAATGGC 
CCCAGCAACC TCGGGCCTAG GGGACTGTGT CCAGCCACGG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_005197904.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197961.1 Bos taurus patched 2 (PTCH2), transcript variant X1, mRNA.

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CloneID	OBa03591
Clone ID Related Accession (Same CDS sequence)	XM_005204892.3 , XM_005197904.3
Accession Version	XM_005197904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGGCTT CCTGACCGCT CAGGGTAGCC GGAACCTGCG GGCTGCCCGG 
GCCCTGGAGC GCACATTTGC TCCAGTGACC GATGGGGCCA TCTCCACGTT GCTAGGTCTG 
CTCATGCTTG CTGGCTCCAA CTTTGACTTC ATCGTAAGGT ACTTCTTTGT GGTGCTGACA 
ATACTTACAC TCCTGGGCCT CCTCCATGGG CTCGTGCTGC TGCCGGTACT GCTGTCCATC 
CTGGGCCCCC CACCAGAGGT GGTACAGATG TACAAGGAGA GCGCAGAGGT CCTGAGCCCC 
CCAGCTCCGC AGGGAGGAGG GCTCAGGTGG GGGGTACCCT CCACCCTGCC CCAGAGCTTT 
GCCAGAGTGA CTACCTCCAT GACTGTGGCC CTCCACCCAC CCCCACTGCC TGGTGCCTAC 
ATCCACCCAG CCTCTGAGGA GCCTACTTGG TCGCCTGCTG CCACACCAGC TGCCAATGGC 
CCCAGCAACC TCGGGCCTAG GGGACTGTGT CCAGCCACGG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197961.1
CDS	332..3976
Translation	MARPPPLGELPPGYTPPGRPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTAQGSRNLRAARALERTFAPVTDGAISTLLGLLMLAGSNFDFIVRYFFVVLTILTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESAEVLSPPAPQGGGLRWGVPSTLPQSFARVTTSMTVALHPPPLPGAYIHPASEEPTWSPAATPAANGPSNLGPRGLCPATG

Target ORF information:

RefSeq Version	XM_005197904.3
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197904.3

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGGCTT CCTGACCGCT CAGGGTAGCC GGAACCTGCG GGCTGCCCGG 
GCCCTGGAGC GCACATTTGC TCCAGTGACC GATGGGGCCA TCTCCACGTT GCTAGGTCTG 
CTCATGCTTG CTGGCTCCAA CTTTGACTTC ATCGTAAGGT ACTTCTTTGT GGTGCTGACA 
ATACTTACAC TCCTGGGCCT CCTCCATGGG CTCGTGCTGC TGCCGGTACT GCTGTCCATC 
CTGGGCCCCC CACCAGAGGT GGTACAGATG TACAAGGAGA GCGCAGAGGT CCTGAGCCCC 
CCAGCTCCGC AGGGAGGAGG GCTCAGGTGG GGGGTACCCT CCACCCTGCC CCAGAGCTTT 
GCCAGAGTGA CTACCTCCAT GACTGTGGCC CTCCACCCAC CCCCACTGCC TGGTGCCTAC 
ATCCACCCAG CCTCTGAGGA GCCTACTTGG TCGCCTGCTG CCACACCAGC TGCCAATGGC 
CCCAGCAACC TCGGGCCTAG GGGACTGTGT CCAGCCACGG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_584648.8 ) cDNA ORF clone, Bos taurus(cattle) -> XP_584648.3 Bos taurus patched 2 (PTCH2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa03592
Clone ID Related Accession (Same CDS sequence)	XM_584648.8 , XM_002686431.5
Accession Version	XM_584648.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_584648.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CGCAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_584648.3
CDS	331..3954
Translation	MARPPPLGELPPGYTPPGRPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTAQGSRNLRAARALERTFAPVTDGAISTLLGLLMLAGSNFDFIVRYFFVVLTILTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESAEVLSPPAPQGGGLRWGVPSTLPQSFARVTTSMTVALHPPPLPGAYIHPASEEPTWSPAATPAANGPSNLGPRGLCPATG

Target ORF information:

RefSeq Version	XM_584648.8
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_584648.8

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CGCAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_002686431.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002686477.1 Bos taurus patched 2 (PTCH2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa03592
Clone ID Related Accession (Same CDS sequence)	XM_584648.8 , XM_002686431.5
Accession Version	XM_002686431.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002686431.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CGCAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002686477.1
CDS	331..3954
Translation	MARPPPLGELPPGYTPPGRPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTAQGSRNLRAARALERTFAPVTDGAISTLLGLLMLAGSNFDFIVRYFFVVLTILTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESAEVLSPPAPQGGGLRWGVPSTLPQSFARVTTSMTVALHPPPLPGAYIHPASEEPTWSPAATPAANGPSNLGPRGLCPATG

Target ORF information:

RefSeq Version	XM_002686431.5
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002686431.5

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CGCAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_005197905.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197962.1 Bos taurus patched 2 (PTCH2), transcript variant X3, mRNA.

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CloneID	OBa03593
Clone ID Related Accession (Same CDS sequence)	XM_005197905.3 , XM_005204893.3
Accession Version	XM_005197905.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGTCCTG GGATCTGAAC AAAATCTGCT ACAAGTCAGG AGTTCCCCTA 
ATTGAAAATG GAATGATTGA GCGGATGATT GAGAAGCTGT TTCCCTGCGT GATCCTCACC 
CCCCTCGACT GCTTCTGGGA GGGAGCCAAA CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC 
CGCCCAGACA TCCAGTGGAC CAACCTGGAC CCAGAGCAGC TGCTGGCAGA GCTGGGCCCC 
TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAC 
GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CACCCAGTGC CCCTAACCAT 
CACAGCAGGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC 
CACAAGTTTA TGCACTGGCA GGAGGAGCTG CTGCTGGGAG GCATGGCCAG AGACCCCCAA 
GGACAGCTGC TGAGGGCAGA GGCCCTGCAG AGCACCTTCC TGCTAATGAG CCCCCGCCAG 
CTCTACGAGC ACTTCCGAGG TGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG 
GCCGGCACGG TCCTTCAGGC CTGGCAGCGA CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG 
CCCGAGAACG CATCCCAGCA GATCCACGCC TTCTCCTCTA CCACCCTGGA CGACATCCTG 
CACGCCTTCT CTGAAGTCAG CGCTGCCCGT GTGGTGGGGG GCTACCTGCT CATGCTAGCC 
TATGCCTGTG TGACAATGCT GCGGTGGGAC TGTGCCCAGT CCCAGGGTGC AGTGGGCCTC 
GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG GCCTCGGGCC TGGGGCTCTG TGCCCTGTTG 
GGCATCGCCT TCAACGCTGC CACTACCCAG GTGCTGCCTT TCTTGGCACT GGGCATCGGC 
GTGGACGACA TATTCCTGCT GGCACACGCC TTCACAGAGG CTCCACCTGG CAGCCCTCTC 
CAGGAGCGCA CGGGGGAGTG TCTGCGACGC ACAGGCACCA GTGTCACACT CACATCCATC 
AACAATATGG TTGCCTTCTT CATGGCCGCC CTAGTTCCCA TCCCTGCACT GCGGGCCTTC 
TCCTTGCAGG CAGCCATAGT GGTGGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA 
GCGGTCCTCA GCCTGGACCT GCGGCGGCGC CACTGCCGGC GCCTGGATGT GCTCTGCTGC 
TTCTCTAGCC CCTGCTCTGC TCGGGTGATT CAGATTCTGC CCCAGGAGCT GGGGAATGGG 
ACGGTACCAG TGGGTGTTGC CCACCTGACT GCCACGGTTC AAGCCTTTGC CCACTGTGAA 
GCCAGCAGCC AACATGTGGT CACCATCTTG CCTCCCCAAG CCCAGCTGGT GCCGCCACCT 
TCTGACCCTT TGGGCTCTGA GCTCTTCAGC CCAGGAGGGT CCACACGGGA CCTTCTAGGG 
CAGGAGGAGG GGACAGGGCA GAAGGCAGCC TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT 
CTTGCCCATT TCGCCCGCTC TCAGTTTGCA CCCTTGTTGC TCCAGTCCCA CACCAAGCTT 
AGTCGCCTTT CCCCACAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CGCAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197962.1
CDS	333..3956
Translation	MARPPPLGELPPGYTPPGRPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTAQGSRNLRAARALERTFAPVTDGAISTLLGLLMLAGSNFDFIVRYFFVVLTILTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESAEVLSPPAPQGGGLRWGVPSTLPQSFARVTTSMTVALHPPPLPGAYIHPASEEPTWSPAATPAANGPSNLGPRGLCPATG

Target ORF information:

RefSeq Version	XM_005197905.3
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197905.3

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGTCCTG GGATCTGAAC AAAATCTGCT ACAAGTCAGG AGTTCCCCTA 
ATTGAAAATG GAATGATTGA GCGGATGATT GAGAAGCTGT TTCCCTGCGT GATCCTCACC 
CCCCTCGACT GCTTCTGGGA GGGAGCCAAA CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC 
CGCCCAGACA TCCAGTGGAC CAACCTGGAC CCAGAGCAGC TGCTGGCAGA GCTGGGCCCC 
TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAC 
GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CACCCAGTGC CCCTAACCAT 
CACAGCAGGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC 
CACAAGTTTA TGCACTGGCA GGAGGAGCTG CTGCTGGGAG GCATGGCCAG AGACCCCCAA 
GGACAGCTGC TGAGGGCAGA GGCCCTGCAG AGCACCTTCC TGCTAATGAG CCCCCGCCAG 
CTCTACGAGC ACTTCCGAGG TGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG 
GCCGGCACGG TCCTTCAGGC CTGGCAGCGA CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG 
CCCGAGAACG CATCCCAGCA GATCCACGCC TTCTCCTCTA CCACCCTGGA CGACATCCTG 
CACGCCTTCT CTGAAGTCAG CGCTGCCCGT GTGGTGGGGG GCTACCTGCT CATGCTAGCC 
TATGCCTGTG TGACAATGCT GCGGTGGGAC TGTGCCCAGT CCCAGGGTGC AGTGGGCCTC 
GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG GCCTCGGGCC TGGGGCTCTG TGCCCTGTTG 
GGCATCGCCT TCAACGCTGC CACTACCCAG GTGCTGCCTT TCTTGGCACT GGGCATCGGC 
GTGGACGACA TATTCCTGCT GGCACACGCC TTCACAGAGG CTCCACCTGG CAGCCCTCTC 
CAGGAGCGCA CGGGGGAGTG TCTGCGACGC ACAGGCACCA GTGTCACACT CACATCCATC 
AACAATATGG TTGCCTTCTT CATGGCCGCC CTAGTTCCCA TCCCTGCACT GCGGGCCTTC 
TCCTTGCAGG CAGCCATAGT GGTGGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA 
GCGGTCCTCA GCCTGGACCT GCGGCGGCGC CACTGCCGGC GCCTGGATGT GCTCTGCTGC 
TTCTCTAGCC CCTGCTCTGC TCGGGTGATT CAGATTCTGC CCCAGGAGCT GGGGAATGGG 
ACGGTACCAG TGGGTGTTGC CCACCTGACT GCCACGGTTC AAGCCTTTGC CCACTGTGAA 
GCCAGCAGCC AACATGTGGT CACCATCTTG CCTCCCCAAG CCCAGCTGGT GCCGCCACCT 
TCTGACCCTT TGGGCTCTGA GCTCTTCAGC CCAGGAGGGT CCACACGGGA CCTTCTAGGG 
CAGGAGGAGG GGACAGGGCA GAAGGCAGCC TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT 
CTTGCCCATT TCGCCCGCTC TCAGTTTGCA CCCTTGTTGC TCCAGTCCCA CACCAAGCTT 
AGTCGCCTTT CCCCACAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CGCAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_005204893.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204950.1 Bos taurus patched 2 (PTCH2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa03593
Clone ID Related Accession (Same CDS sequence)	XM_005197905.3 , XM_005204893.3
Accession Version	XM_005204893.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGTCCTG GGATCTGAAC AAAATCTGCT ACAAGTCAGG AGTTCCCCTA 
ATTGAAAATG GAATGATTGA GCGGATGATT GAGAAGCTGT TTCCCTGCGT GATCCTCACC 
CCCCTCGACT GCTTCTGGGA GGGAGCCAAA CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC 
CGCCCAGACA TCCAGTGGAC CAACCTGGAC CCAGAGCAGC TGCTGGCAGA GCTGGGCCCC 
TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAC 
GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CACCCAGTGC CCCTAACCAT 
CACAGCAGGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC 
CACAAGTTTA TGCACTGGCA GGAGGAGCTG CTGCTGGGAG GCATGGCCAG AGACCCCCAA 
GGACAGCTGC TGAGGGCAGA GGCCCTGCAG AGCACCTTCC TGCTAATGAG CCCCCGCCAG 
CTCTACGAGC ACTTCCGAGG TGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG 
GCCGGCACGG TCCTTCAGGC CTGGCAGCGA CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG 
CCCGAGAACG CATCCCAGCA GATCCACGCC TTCTCCTCTA CCACCCTGGA CGACATCCTG 
CACGCCTTCT CTGAAGTCAG CGCTGCCCGT GTGGTGGGGG GCTACCTGCT CATGCTAGCC 
TATGCCTGTG TGACAATGCT GCGGTGGGAC TGTGCCCAGT CCCAGGGTGC AGTGGGCCTC 
GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG GCCTCGGGCC TGGGGCTCTG TGCCCTGTTG 
GGCATCGCCT TCAACGCTGC CACTACCCAG GTGCTGCCTT TCTTGGCACT GGGCATCGGC 
GTGGACGACA TATTCCTGCT GGCACACGCC TTCACAGAGG CTCCACCTGG CAGCCCTCTC 
CAGGAGCGCA CGGGGGAGTG TCTGCGACGC ACAGGCACCA GTGTCACACT CACATCCATC 
AACAATATGG TTGCCTTCTT CATGGCCGCC CTAGTTCCCA TCCCTGCACT GCGGGCCTTC 
TCCTTGCAGG CAGCCATAGT GGTGGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA 
GCGGTCCTCA GCCTGGACCT GCGGCGGCGC CACTGCCGGC GCCTGGATGT GCTCTGCTGC 
TTCTCTAGCC CCTGCTCTGC TCGGGTGATT CAGATTCTGC CCCAGGAGCT GGGGAATGGG 
ACGGTACCAG TGGGTGTTGC CCACCTGACT GCCACGGTTC AAGCCTTTGC CCACTGTGAA 
GCCAGCAGCC AACATGTGGT CACCATCTTG CCTCCCCAAG CCCAGCTGGT GCCGCCACCT 
TCTGACCCTT TGGGCTCTGA GCTCTTCAGC CCAGGAGGGT CCACACGGGA CCTTCTAGGG 
CAGGAGGAGG GGACAGGGCA GAAGGCAGCC TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT 
CTTGCCCATT TCGCCCGCTC TCAGTTTGCA CCCTTGTTGC TCCAGTCCCA CACCAAGCTT 
AGTCGCCTTT CCCCACAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CGCAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204950.1
CDS	333..3956
Translation	MARPPPLGELPPGYTPPGRPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTAQGSRNLRAARALERTFAPVTDGAISTLLGLLMLAGSNFDFIVRYFFVVLTILTLLGLLHGLVLLPVLLSILGPPPEVVQMYKESAEVLSPPAPQGGGLRWGVPSTLPQSFARVTTSMTVALHPPPLPGAYIHPASEEPTWSPAATPAANGPSNLGPRGLCPATG

Target ORF information:

RefSeq Version	XM_005204893.3
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204893.3

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGTCCTG GGATCTGAAC AAAATCTGCT ACAAGTCAGG AGTTCCCCTA 
ATTGAAAATG GAATGATTGA GCGGATGATT GAGAAGCTGT TTCCCTGCGT GATCCTCACC 
CCCCTCGACT GCTTCTGGGA GGGAGCCAAA CTCCAAGGGG GCTCTGCCTA CTTGCCGGGC 
CGCCCAGACA TCCAGTGGAC CAACCTGGAC CCAGAGCAGC TGCTGGCAGA GCTGGGCCCC 
TTTGCCTCCC TTGAGGGCTT CCGGGAGCTG CTAGACAAGG CACAGGTGGG CCAGGCCTAC 
GTGGGGCGGC CCTGTCTGCA CCCTGACGAC CTCCACTGCC CACCCAGTGC CCCTAACCAT 
CACAGCAGGC AGGCTCCCAA CGTGGCTCAG GAGTTGAGTG GGGGCTGCCA TGGCTTCTCC 
CACAAGTTTA TGCACTGGCA GGAGGAGCTG CTGCTGGGAG GCATGGCCAG AGACCCCCAA 
GGACAGCTGC TGAGGGCAGA GGCCCTGCAG AGCACCTTCC TGCTAATGAG CCCCCGCCAG 
CTCTACGAGC ACTTCCGAGG TGACTACCAG ACGCATGACA TCGGCTGGAG CGAGGAGCAG 
GCCGGCACGG TCCTTCAGGC CTGGCAGCGA CGCTTTGTGC AGCTGGCCCA GGAGGCCCTG 
CCCGAGAACG CATCCCAGCA GATCCACGCC TTCTCCTCTA CCACCCTGGA CGACATCCTG 
CACGCCTTCT CTGAAGTCAG CGCTGCCCGT GTGGTGGGGG GCTACCTGCT CATGCTAGCC 
TATGCCTGTG TGACAATGCT GCGGTGGGAC TGTGCCCAGT CCCAGGGTGC AGTGGGCCTC 
GCTGGTGTGC TGCTGGTGGC CCTGGCAGTG GCCTCGGGCC TGGGGCTCTG TGCCCTGTTG 
GGCATCGCCT TCAACGCTGC CACTACCCAG GTGCTGCCTT TCTTGGCACT GGGCATCGGC 
GTGGACGACA TATTCCTGCT GGCACACGCC TTCACAGAGG CTCCACCTGG CAGCCCTCTC 
CAGGAGCGCA CGGGGGAGTG TCTGCGACGC ACAGGCACCA GTGTCACACT CACATCCATC 
AACAATATGG TTGCCTTCTT CATGGCCGCC CTAGTTCCCA TCCCTGCACT GCGGGCCTTC 
TCCTTGCAGG CAGCCATAGT GGTGGGCTGC AACTTTGCAG CCGTGATGCT TGTCTTCCCA 
GCGGTCCTCA GCCTGGACCT GCGGCGGCGC CACTGCCGGC GCCTGGATGT GCTCTGCTGC 
TTCTCTAGCC CCTGCTCTGC TCGGGTGATT CAGATTCTGC CCCAGGAGCT GGGGAATGGG 
ACGGTACCAG TGGGTGTTGC CCACCTGACT GCCACGGTTC AAGCCTTTGC CCACTGTGAA 
GCCAGCAGCC AACATGTGGT CACCATCTTG CCTCCCCAAG CCCAGCTGGT GCCGCCACCT 
TCTGACCCTT TGGGCTCTGA GCTCTTCAGC CCAGGAGGGT CCACACGGGA CCTTCTAGGG 
CAGGAGGAGG GGACAGGGCA GAAGGCAGCC TGCAAGTCCC TGCCCTGTGC CCGCTGGAAT 
CTTGCCCATT TCGCCCGCTC TCAGTTTGCA CCCTTGTTGC TCCAGTCCCA CACCAAGCTT 
AGTCGCCTTT CCCCACAGGC TGTAGTACTG GTACTTTTTG GGGCTCTCCT GGGCCTGAGC 
CTCTATGGAG CGACCTTGGT GCAGGACGGT CTGGCCCTGA CTGATGTGGT GCCGCGCGGC 
ACCAAGGAGC ATGCCTTCCT GAGCGCCCAG CTCAGGTACT TCTCTCTGTA TGAGGTGGCT 
CTGGTGACAC AGGGTGGCTT TGACTACGCC CACTCCCAAC GCGCCCTCTT TGATCTGCAC 
CAGCGCTTCA GTTCCCTCAA GGCCGTGCTG CCCCCACCGG CCACCCAGGC ACCCCGCACC 
TGGCTGCACT ACTACCGCAG CTGGCTACAG GGAATCCAGG CTGCATTTGA CCAGGACTGG 
GCTTCTGGGC GCATCACCCG CCACTCATAC CGCAATGGCT CTGAGGATGG GGCCCTGGCA 
TACAAGCTGC TCGTCCAGAC CGGGGATGCC CAGGAGCCTC TAGATTTCAG CCAGCTGACC 
ACCAAGAAGC TGGTGGACAA GGAAGGGCTG ATCCCACCCG AGCTCTTCTA CATGGGGCTG 
ACCGTGTGGG TGAGCAGTGA CCCCCTGGGT CTGGCAGCCT CACAGGCCAA CTTCTATCCC 
CCACCTCCTG AGTGGCTGCA TGACAAGTAT GACACCACGG GGGAGAATCT TCGCATCCCG 
GCGGCCCAGC CCTTGGAGTT TGCCCAGTTC CCCTTCCTCC TGCGCGGCCT CCAGAAGACT 
GCAGACTTCG TGGAGGCCAT TGAGGGGGCC CGGGCAGCGT GTGCTGAGGC CAGCCAGGCC 
GGGGTGCATG CCTACCCCAG CGGCTCCCCC TTCCTCTTCT GGGAGCAGTA CCTGGGCCTG 
CGGCGCTGCT TCCTGCTGGC AGTCTGCATC CTGCTGCTGT GTACTTTCCT CGTCTGTGCC 
CTGCTGCTGC TCAACCCCTG GACGGCTGCG CTCATAGTAC TGGTCCTGGC GATGATGACT 
GTGGAGCTCT TTGGCATCAT GGGTTTCCTG GGCATCAAGC TGAGTGCCAT CCCGGTGGTG 
ATTCTTGTGG CCTCCATAGG CATCGGTGTC GAGTTCACCG TCCACGTGGC TCTGGGCTTC 
CTGACCGCTC AGGGTAGCCG GAACCTGCGG GCTGCCCGGG CCCTGGAGCG CACATTTGCT 
CCAGTGACCG ATGGGGCCAT CTCCACGTTG CTAGGTCTGC TCATGCTTGC TGGCTCCAAC 
TTTGACTTCA TCGTAAGGTA CTTCTTTGTG GTGCTGACAA TACTTACACT CCTGGGCCTC 
CTCCATGGGC TCGTGCTGCT GCCGGTACTG CTGTCCATCC TGGGCCCCCC ACCAGAGGTG 
GTACAGATGT ACAAGGAGAG CGCAGAGGTC CTGAGCCCCC CAGCTCCGCA GGGAGGAGGG 
CTCAGGTGGG GGGTACCCTC CACCCTGCCC CAGAGCTTTG CCAGAGTGAC TACCTCCATG 
ACTGTGGCCC TCCACCCACC CCCACTGCCT GGTGCCTACA TCCACCCAGC CTCTGAGGAG 
CCTACTTGGT CGCCTGCTGC CACACCAGCT GCCAATGGCC CCAGCAACCT CGGGCCTAGG 
GGACTGTGTC CAGCCACGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_010803747.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802049.1 Bos taurus patched 2 (PTCH2), transcript variant X4, mRNA.

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CloneID	OBa47010
Clone ID Related Accession (Same CDS sequence)	XM_010803747.2 , XM_010827054.2
Accession Version	XM_010803747.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGAC 
CACACCATCC TGCTCCCAGC CCACGGGGCG GGGTGGGATA GAACCAAAAC AAAACACCCT 
CAGTGGCCAA CAGACAGGGC TCAACTCACA GGACCCCTTC CCCATGAAGT ACCACCAGCT 
GAAGTTAGCA GCCTCCCCTT TTGCCCAGAC ATCATGTCCC TGCCCTCCAG CCCTCCTAAC 
TTCTTCTTGA GACCCACCTT AGCCTTTAGG TGCAGGATTG GCCCCTGGTC TCAACATCCT 
GTCCCTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802049.1
CDS	396..3887
Translation	MARPPPLGELPPGYTPPGRPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALVLLCGADNTYTPGPPPWARAAAGTAVHPGPPTRGDHTILLPAHGAGWDRTKTKHPQWPTDRAQLTGPLPHEVPPAEVSSLPFCPDIMSLPSSPPNFFLRPTLAFRCRIGPWSQHPVPC

Target ORF information:

RefSeq Version	XM_010803747.2
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803747.2

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGAC 
CACACCATCC TGCTCCCAGC CCACGGGGCG GGGTGGGATA GAACCAAAAC AAAACACCCT 
CAGTGGCCAA CAGACAGGGC TCAACTCACA GGACCCCTTC CCCATGAAGT ACCACCAGCT 
GAAGTTAGCA GCCTCCCCTT TTGCCCAGAC ATCATGTCCC TGCCCTCCAG CCCTCCTAAC 
TTCTTCTTGA GACCCACCTT AGCCTTTAGG TGCAGGATTG GCCCCTGGTC TCAACATCCT 
GTCCCTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_010827054.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825356.1 Bos taurus patched 2 (PTCH2), transcript variant X4, mRNA.

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CloneID	OBa47010
Clone ID Related Accession (Same CDS sequence)	XM_010803747.2 , XM_010827054.2
Accession Version	XM_010827054.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827054.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGAC 
CACACCATCC TGCTCCCAGC CCACGGGGCG GGGTGGGATA GAACCAAAAC AAAACACCCT 
CAGTGGCCAA CAGACAGGGC TCAACTCACA GGACCCCTTC CCCATGAAGT ACCACCAGCT 
GAAGTTAGCA GCCTCCCCTT TTGCCCAGAC ATCATGTCCC TGCCCTCCAG CCCTCCTAAC 
TTCTTCTTGA GACCCACCTT AGCCTTTAGG TGCAGGATTG GCCCCTGGTC TCAACATCCT 
GTCCCTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825356.1
CDS	396..3887
Translation	MARPPPLGELPPGYTPPGRPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALVLLCGADNTYTPGPPPWARAAAGTAVHPGPPTRGDHTILLPAHGAGWDRTKTKHPQWPTDRAQLTGPLPHEVPPAEVSSLPFCPDIMSLPSSPPNFFLRPTLAFRCRIGPWSQHPVPC

Target ORF information:

RefSeq Version	XM_010827054.2
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010827054.2

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGAC 
CACACCATCC TGCTCCCAGC CCACGGGGCG GGGTGGGATA GAACCAAAAC AAAACACCCT 
CAGTGGCCAA CAGACAGGGC TCAACTCACA GGACCCCTTC CCCATGAAGT ACCACCAGCT 
GAAGTTAGCA GCCTCCCCTT TTGCCCAGAC ATCATGTCCC TGCCCTCCAG CCCTCCTAAC 
TTCTTCTTGA GACCCACCTT AGCCTTTAGG TGCAGGATTG GCCCCTGGTC TCAACATCCT 
GTCCCTTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_010803749.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802051.1 Bos taurus patched 2 (PTCH2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa47011
Clone ID Related Accession (Same CDS sequence)	XM_010803749.2 , XM_010827055.2
Accession Version	XM_010803749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803749.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGGT 
ACAGATGTAC AAGGAGAGCG CAGAGGTCCT GAGCCCCCCA GCTCCGCAGG GAGGAGGGCT 
CAGGTGGGGG GTACCCTCCA CCCTGCCCCA GAGCTTTGCC AGAGTGACTA CCTCCATGAC 
TGTGGCCCTC CACCCACCCC CACTGCCTGG TGCCTACATC CACCCAGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802051.1
CDS	354..3767
Translation	MARPPPLGELPPGYTPPGRPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALVLLCGADNTYTPGPPPWARAAAGTAVHPGPPTRGGTDVQGERRGPEPPSSAGRRAQVGGTLHPAPELCQSDYLHDCGPPPTPTAWCLHPPSL

Target ORF information:

RefSeq Version	XM_010803749.2
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803749.2

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGGT 
ACAGATGTAC AAGGAGAGCG CAGAGGTCCT GAGCCCCCCA GCTCCGCAGG GAGGAGGGCT 
CAGGTGGGGG GTACCCTCCA CCCTGCCCCA GAGCTTTGCC AGAGTGACTA CCTCCATGAC 
TGTGGCCCTC CACCCACCCC CACTGCCTGG TGCCTACATC CACCCAGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_010827055.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010825357.1 Bos taurus patched 2 (PTCH2), transcript variant X5, mRNA.

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CloneID	OBa47011
Clone ID Related Accession (Same CDS sequence)	XM_010803749.2 , XM_010827055.2
Accession Version	XM_010827055.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein patched homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644667.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGGT 
ACAGATGTAC AAGGAGAGCG CAGAGGTCCT GAGCCCCCCA GCTCCGCAGG GAGGAGGGCT 
CAGGTGGGGG GTACCCTCCA CCCTGCCCCA GAGCTTTGCC AGAGTGACTA CCTCCATGAC 
TGTGGCCCTC CACCCACCCC CACTGCCTGG TGCCTACATC CACCCAGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010825357.1
CDS	354..3767
Translation	MARPPPLGELPPGYTPPGRPATPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALALGLRVAIIETDLEQLWVEVGSRVSQELEYTKEKLGEEAAYTSQMLIQTPRQEGENVLTPEALDLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLAELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELSGGCHGFSHKFMHWQEELLLGGMARDPQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAGTVLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLGIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEAPPGSPLQERTGECLRRTGTSVTLTSINNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAVLSLDLRRRHCRRLDVLCCFSSPCSARVIQILPQELGNGTVPVGVAHLTATVQAFAHCEASSQHVVTILPPQAQLVPPPSDPLGSELFSPGGSTRDLLGQEEGTGQKAACKSLPCARWNLAHFARSQFAPLLLQSHTKLSRLSPQAVVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLVQTGDAQEPLDFSQLTTKKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQKTADFVEAIEGARAACAEASQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLLCTFLVCALLLLNPWTAALIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALVLLCGADNTYTPGPPPWARAAAGTAVHPGPPTRGGTDVQGERRGPEPPSSAGRRAQVGGTLHPAPELCQSDYLHDCGPPPTPTAWCLHPPSL

Target ORF information:

RefSeq Version	XM_010827055.2
Organism	Bos taurus(cattle)
Definition	Bos taurus patched 2 (PTCH2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010827055.2

ORF Insert Sequence:

ATGGCTCGGC CGCCACCTCT CGGGGAGCTG CCCCCGGGCT ACACACCCCC AGGTCGACCT 
GCAACACCCC AGATCCTAGC TGGGAGCCTG AAGGCTCCGC TCTGGCTCCG TGCTTACTTC 
CAGGGCCTGC TCTTCTCTTT GGGCTGCGGG ATCCAGAGAC ACTGTGGCAA AGTGCTCTTC 
CTGGGACTGT TGGCCTTTGG GGCCCTAGCA CTGGGTCTCC GCGTGGCCAT CATTGAGACA 
GACCTAGAAC AGCTCTGGGT GGAAGTGGGC AGCCGGGTGA GCCAGGAACT GGAATACACC 
AAGGAGAAGC TGGGGGAGGA GGCTGCGTAT ACCTCCCAGA TGTTGATACA GACCCCGCGC 
CAGGAGGGGG AGAACGTCCT CACGCCCGAG GCACTTGACC TCCACCTCCA GGCAGCCCTC 
ACCGCCAGTA AAGTGCAAGT ATCCCTCTAT GGAAAGTCCT GGGATCTGAA CAAAATCTGC 
TACAAGTCAG GAGTTCCCCT AATTGAAAAT GGAATGATTG AGCGGATGAT TGAGAAGCTG 
TTTCCCTGCG TGATCCTCAC CCCCCTCGAC TGCTTCTGGG AGGGAGCCAA ACTCCAAGGG 
GGCTCTGCCT ACTTGCCGGG CCGCCCAGAC ATCCAGTGGA CCAACCTGGA CCCAGAGCAG 
CTGCTGGCAG AGCTGGGCCC CTTTGCCTCC CTTGAGGGCT TCCGGGAGCT GCTAGACAAG 
GCACAGGTGG GCCAGGCCTA CGTGGGGCGG CCCTGTCTGC ACCCTGACGA CCTCCACTGC 
CCACCCAGTG CCCCTAACCA TCACAGCAGG CAGGCTCCCA ACGTGGCTCA GGAGTTGAGT 
GGGGGCTGCC ATGGCTTCTC CCACAAGTTT ATGCACTGGC AGGAGGAGCT GCTGCTGGGA 
GGCATGGCCA GAGACCCCCA AGGACAGCTG CTGAGGGCAG AGGCCCTGCA GAGCACCTTC 
CTGCTAATGA GCCCCCGCCA GCTCTACGAG CACTTCCGAG GTGACTACCA GACGCATGAC 
ATCGGCTGGA GCGAGGAGCA GGCCGGCACG GTCCTTCAGG CCTGGCAGCG ACGCTTTGTG 
CAGCTGGCCC AGGAGGCCCT GCCCGAGAAC GCATCCCAGC AGATCCACGC CTTCTCCTCT 
ACCACCCTGG ACGACATCCT GCACGCCTTC TCTGAAGTCA GCGCTGCCCG TGTGGTGGGG 
GGCTACCTGC TCATGCTAGC CTATGCCTGT GTGACAATGC TGCGGTGGGA CTGTGCCCAG 
TCCCAGGGTG CAGTGGGCCT CGCTGGTGTG CTGCTGGTGG CCCTGGCAGT GGCCTCGGGC 
CTGGGGCTCT GTGCCCTGTT GGGCATCGCC TTCAACGCTG CCACTACCCA GGTGCTGCCT 
TTCTTGGCAC TGGGCATCGG CGTGGACGAC ATATTCCTGC TGGCACACGC CTTCACAGAG 
GCTCCACCTG GCAGCCCTCT CCAGGAGCGC ACGGGGGAGT GTCTGCGACG CACAGGCACC 
AGTGTCACAC TCACATCCAT CAACAATATG GTTGCCTTCT TCATGGCCGC CCTAGTTCCC 
ATCCCTGCAC TGCGGGCCTT CTCCTTGCAG GCAGCCATAG TGGTGGGCTG CAACTTTGCA 
GCCGTGATGC TTGTCTTCCC AGCGGTCCTC AGCCTGGACC TGCGGCGGCG CCACTGCCGG 
CGCCTGGATG TGCTCTGCTG CTTCTCTAGC CCCTGCTCTG CTCGGGTGAT TCAGATTCTG 
CCCCAGGAGC TGGGGAATGG GACGGTACCA GTGGGTGTTG CCCACCTGAC TGCCACGGTT 
CAAGCCTTTG CCCACTGTGA AGCCAGCAGC CAACATGTGG TCACCATCTT GCCTCCCCAA 
GCCCAGCTGG TGCCGCCACC TTCTGACCCT TTGGGCTCTG AGCTCTTCAG CCCAGGAGGG 
TCCACACGGG ACCTTCTAGG GCAGGAGGAG GGGACAGGGC AGAAGGCAGC CTGCAAGTCC 
CTGCCCTGTG CCCGCTGGAA TCTTGCCCAT TTCGCCCGCT CTCAGTTTGC ACCCTTGTTG 
CTCCAGTCCC ACACCAAGCT TAGTCGCCTT TCCCCACAGG CTGTAGTACT GGTACTTTTT 
GGGGCTCTCC TGGGCCTGAG CCTCTATGGA GCGACCTTGG TGCAGGACGG TCTGGCCCTG 
ACTGATGTGG TGCCGCGCGG CACCAAGGAG CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCTCTGT ATGAGGTGGC TCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGTTCCCTCA AGGCCGTGCT GCCCCCACCG 
GCCACCCAGG CACCCCGCAC CTGGCTGCAC TACTACCGCA GCTGGCTACA GGGAATCCAG 
GCTGCATTTG ACCAGGACTG GGCTTCTGGG CGCATCACCC GCCACTCATA CCGCAATGGC 
TCTGAGGATG GGGCCCTGGC ATACAAGCTG CTCGTCCAGA CCGGGGATGC CCAGGAGCCT 
CTAGATTTCA GCCAGCTGAC CACCAAGAAG CTGGTGGACA AGGAAGGGCT GATCCCACCC 
GAGCTCTTCT ACATGGGGCT GACCGTGTGG GTGAGCAGTG ACCCCCTGGG TCTGGCAGCC 
TCACAGGCCA ACTTCTATCC CCCACCTCCT GAGTGGCTGC ATGACAAGTA TGACACCACG 
GGGGAGAATC TTCGCATCCC GGCGGCCCAG CCCTTGGAGT TTGCCCAGTT CCCCTTCCTC 
CTGCGCGGCC TCCAGAAGAC TGCAGACTTC GTGGAGGCCA TTGAGGGGGC CCGGGCAGCG 
TGTGCTGAGG CCAGCCAGGC CGGGGTGCAT GCCTACCCCA GCGGCTCCCC CTTCCTCTTC 
TGGGAGCAGT ACCTGGGCCT GCGGCGCTGC TTCCTGCTGG CAGTCTGCAT CCTGCTGCTG 
TGTACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCCT GGACGGCTGC GCTCATAGTA 
CTGGTCCTGG CGATGATGAC TGTGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGTGCCA TCCCGGTGGT GATTCTTGTG GCCTCCATAG GCATCGGTGT CGAGTTCACC 
GTCCACGTGG CTCTGGTACT TCTTTGTGGT GCTGACAATA CTTACACTCC TGGGCCTCCT 
CCATGGGCTC GTGCTGCTGC CGGTACTGCT GTCCATCCTG GGCCCCCCAC CAGAGGTGGT 
ACAGATGTAC AAGGAGAGCG CAGAGGTCCT GAGCCCCCCA GCTCCGCAGG GAGGAGGGCT 
CAGGTGGGGG GTACCCTCCA CCCTGCCCCA GAGCTTTGCC AGAGTGACTA CCTCCATGAC 
TGTGGCCCTC CACCCACCCC CACTGCCTGG TGCCTACATC CACCCAGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PTCH2
Entrez Gene ID	507948
Full Name	patched 2
General protein information	Preferred Names patched 2 Names protein patched homolog 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	PTCH2	NP_003729.3
Canis lupus familiaris (dog)	PTCH2	XP_539636.3
Mus musculus (house mouse)	Ptch2	NP_032984.1
Pan troglodytes (chimpanzee)	PTCH2	XP_003954411.1
Bos taurus (cattle)	PTCH2	XP_005204949.1

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PTCH2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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