MISP cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MISP
Entrez Gene ID	505316
Full Name	mitotic spindle positioning
Synonyms	C7H19orf21
General protein information	Preferred Names mitotic spindle positioning Names mitotic interactor and substrate of PLK1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005209261.3	XP_005209318.1	mitotic interactor and substrate of PLK1 isoform X1
XM_005209259.2	XP_005209316.1	mitotic interactor and substrate of PLK1 isoform X1
XM_005209260.4	XP_005209317.1	mitotic interactor and substrate of PLK1 isoform X1
XM_005209260.3	XP_005209317.1	mitotic interactor and substrate of PLK1 isoform X1
XM_005209261.4	XP_005209318.1	mitotic interactor and substrate of PLK1 isoform X1
NM_001035269.2	NP_001030346.1	mitotic interactor and substrate of PLK1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	C28H19ORF21	XP_418218.4
Pan troglodytes (chimpanzee)	MISP	XP_001146667.1
Bos taurus (cattle)	MISP	NP_001030346.1
Xenopus tropicalis (tropical clawed frog)	misp	XP_002937429.1
Homo sapiens (human)	MISP	NP_775752.1
Mus musculus (house mouse)	Misp	NP_084494.1
Rattus norvegicus (Norway rat)	Misp	NP_001102754.1

Gene Ontology

The following MISP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MISP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36819	NM_001035269.2 Latest version!	Bos taurus mitotic spindle positioning (MISP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa21400	XM_005209261.4 Latest version!	Bos taurus mitotic spindle positioning (MISP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa21400	XM_005209259.2 Latest version!	Bos taurus mitotic spindle positioning (MISP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa21400	XM_005209260.4 Latest version!	Bos taurus mitotic spindle positioning (MISP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa21400	XM_005209260.3	Bos taurus mitotic spindle positioning (MISP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa21400	XM_005209261.3	Bos taurus mitotic spindle positioning (MISP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MISP ( NM_001035269.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001030346.1 Bos taurus mitotic spindle positioning (MISP), mRNA.

Price & Availability↑

CloneID	OBa36819
Clone ID Related Accession (Same CDS sequence)	NM_001035269.2
Accession Version	NM_001035269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	mitotic interactor and substrate of PLK1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC107524.1. On Aug 30, 2012 this sequence version replaced NM_001035269.1. ##Evidence-Data-START## Transcript exon combination :: BC107524.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145446 [ECO:0000348] ##Evidence-Data-END##

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACATCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCACCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030346.1
CDS	150..2219
Translation	MDRVTRYPIFGIPHSSRMAGVAFDGDTSYTFELVGVGPTASFWSQDETPAWPTDHEASLEMTRTGTSRSLRVFPGRMAPWPPCPQDNEDEEVKAYHLEARDIPPWQPQNLERERQAVIRGQAVRKGGTVATLRGASDHREPRGPSRPQSMPLEENEIDREQIDFLAVRQQFLNLEQANPPPGAAHANARVLQSPQSKVAHAKTGVSRNAPLGVAHVNSEVPQSPQPKVAHVNAVASQNSPPRASQASKALSRPPLANGYVLPVKPQVKEVVTEEKRVHPLPTRSVAQALEDPGSRSREESPEPLKETPIEREIRLAQEREADLREQRGLQRATSHQELVEIPARLLLSKVSLAAAPRRDRGRPSLYVQRDIAQESQREEDHRREGLQVGRASTPDWSSEGPQPRLRRVHSSDSILSPTPDAHAASLAPEVRKVSRIPPDAYQPYLSPGTPPGESPASHAYCRPSNLSADEARPVGSPKAAGSQRHPSESSAKPSGLKQEPPRGPLHASRGVLRQEYFHLRPLRFRVPDEPERAEATRVWGWEVAGAPALRLQKSQSSELLEREVENVLRREREMAEERRSALYPEVFSDECCDHDSRSSSRKSSITGSYSVSESHYFTPIHLHSDLVWTVEAPAKDALQQKKKKEQWYAGINPLDDVNSEILEATRVTRHRNAMAERWEAGIYASEDED

Target ORF information:

RefSeq Version	NM_001035269.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mitotic spindle positioning (MISP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001035269.2

ORF Insert Sequence:

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACATCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCACCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MISP ( XM_005209261.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209318.1 Bos taurus mitotic spindle positioning (MISP), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa21400
Clone ID Related Accession (Same CDS sequence)	XM_005209261.3 , XM_005209259.2 , XM_005209260.4 , XM_005209260.3 , XM_005209261.4
Accession Version	XM_005209261.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mitotic interactor and substrate of PLK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACACCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCCCCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209318.1
CDS	217..2286
Translation	MDRVTRYPIFGIPHSSRMAGVAFDGDTSYTFELVGVGPTASFWSQDETPAWPTDHEASLEMTRTGTSRSLRVFPGRMAPWPPCPQDNEDEEVKAYHLEARDTPPWQPQNLERERQAVIRGQAVRKGGTVATLRGASDHREPRGPSRPQSMPLEENEIDREQIDFLAVRQQFLNLEQANPPPGAAHANARVLQSPQSKVAHAKTGVSRNAPLGVAHVNSEVPQSPQPKVAHVNAVASQNSPPRASQASKALSRPPLANGYVLPVKPQVKEVVTEEKRVHPLPTRSVAQALEDPGSRSREESPEPLKETPIEREIRLAQEREADLREQRGLQRATSHQELVEIPARLLLSKVSLAAAPRRDRGRPSLYVQRDIAQESQREEDHRREGLQVGRASTPDWSSEGPQPRLRRVHSSDSILSPTPDAHAASLAPEVRKVSRIPPDAYQPYLSPGTPPGESPASHAYCRPSNLSADEARPVGSPKAAGSQRHPSESSAKPSGLKQEPPRGPLHASRGVLRQEYFHLRPLRFRVPDEPERAEAPRVWGWEVAGAPALRLQKSQSSELLEREVENVLRREREMAEERRSALYPEVFSDECCDHDSRSSSRKSSITGSYSVSESHYFTPIHLHSDLVWTVEAPAKDALQQKKKKEQWYAGINPLDDVNSEILEATRVTRHRNAMAERWEAGIYASEDED

Target ORF information:

RefSeq Version	XM_005209261.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mitotic spindle positioning (MISP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209261.3

ORF Insert Sequence:

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACACCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCCCCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MISP ( XM_005209259.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209316.1 Bos taurus mitotic spindle positioning (MISP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21400
Clone ID Related Accession (Same CDS sequence)	XM_005209261.3 , XM_005209259.2 , XM_005209260.4 , XM_005209260.3 , XM_005209261.4
Accession Version	XM_005209259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mitotic interactor and substrate of PLK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005209259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACACCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCCCCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209316.1
CDS	226..2295
Translation	MDRVTRYPIFGIPHSSRMAGVAFDGDTSYTFELVGVGPTASFWSQDETPAWPTDHEASLEMTRTGTSRSLRVFPGRMAPWPPCPQDNEDEEVKAYHLEARDTPPWQPQNLERERQAVIRGQAVRKGGTVATLRGASDHREPRGPSRPQSMPLEENEIDREQIDFLAVRQQFLNLEQANPPPGAAHANARVLQSPQSKVAHAKTGVSRNAPLGVAHVNSEVPQSPQPKVAHVNAVASQNSPPRASQASKALSRPPLANGYVLPVKPQVKEVVTEEKRVHPLPTRSVAQALEDPGSRSREESPEPLKETPIEREIRLAQEREADLREQRGLQRATSHQELVEIPARLLLSKVSLAAAPRRDRGRPSLYVQRDIAQESQREEDHRREGLQVGRASTPDWSSEGPQPRLRRVHSSDSILSPTPDAHAASLAPEVRKVSRIPPDAYQPYLSPGTPPGESPASHAYCRPSNLSADEARPVGSPKAAGSQRHPSESSAKPSGLKQEPPRGPLHASRGVLRQEYFHLRPLRFRVPDEPERAEAPRVWGWEVAGAPALRLQKSQSSELLEREVENVLRREREMAEERRSALYPEVFSDECCDHDSRSSSRKSSITGSYSVSESHYFTPIHLHSDLVWTVEAPAKDALQQKKKKEQWYAGINPLDDVNSEILEATRVTRHRNAMAERWEAGIYASEDED

Target ORF information:

RefSeq Version	XM_005209259.2
Organism	Bos taurus(cattle)
Definition	Bos taurus mitotic spindle positioning (MISP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209259.2

ORF Insert Sequence:

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACACCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCCCCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MISP ( XM_005209260.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209317.1 Bos taurus mitotic spindle positioning (MISP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa21400
Clone ID Related Accession (Same CDS sequence)	XM_005209261.3 , XM_005209259.2 , XM_005209260.4 , XM_005209260.3 , XM_005209261.4
Accession Version	XM_005209260.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mitotic interactor and substrate of PLK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209260.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACACCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCCCCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209317.1
CDS	152..2221
Translation	MDRVTRYPIFGIPHSSRMAGVAFDGDTSYTFELVGVGPTASFWSQDETPAWPTDHEASLEMTRTGTSRSLRVFPGRMAPWPPCPQDNEDEEVKAYHLEARDTPPWQPQNLERERQAVIRGQAVRKGGTVATLRGASDHREPRGPSRPQSMPLEENEIDREQIDFLAVRQQFLNLEQANPPPGAAHANARVLQSPQSKVAHAKTGVSRNAPLGVAHVNSEVPQSPQPKVAHVNAVASQNSPPRASQASKALSRPPLANGYVLPVKPQVKEVVTEEKRVHPLPTRSVAQALEDPGSRSREESPEPLKETPIEREIRLAQEREADLREQRGLQRATSHQELVEIPARLLLSKVSLAAAPRRDRGRPSLYVQRDIAQESQREEDHRREGLQVGRASTPDWSSEGPQPRLRRVHSSDSILSPTPDAHAASLAPEVRKVSRIPPDAYQPYLSPGTPPGESPASHAYCRPSNLSADEARPVGSPKAAGSQRHPSESSAKPSGLKQEPPRGPLHASRGVLRQEYFHLRPLRFRVPDEPERAEAPRVWGWEVAGAPALRLQKSQSSELLEREVENVLRREREMAEERRSALYPEVFSDECCDHDSRSSSRKSSITGSYSVSESHYFTPIHLHSDLVWTVEAPAKDALQQKKKKEQWYAGINPLDDVNSEILEATRVTRHRNAMAERWEAGIYASEDED

Target ORF information:

RefSeq Version	XM_005209260.4
Organism	Bos taurus(cattle)
Definition	Bos taurus mitotic spindle positioning (MISP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209260.4

ORF Insert Sequence:

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACACCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCCCCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MISP ( XM_005209260.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209317.1 Bos taurus mitotic spindle positioning (MISP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21400
Clone ID Related Accession (Same CDS sequence)	XM_005209261.3 , XM_005209259.2 , XM_005209260.4 , XM_005209260.3 , XM_005209261.4
Accession Version	XM_005209260.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mitotic interactor and substrate of PLK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209260.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACACCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCCCCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209317.1
CDS	1373..3442
Translation	MDRVTRYPIFGIPHSSRMAGVAFDGDTSYTFELVGVGPTASFWSQDETPAWPTDHEASLEMTRTGTSRSLRVFPGRMAPWPPCPQDNEDEEVKAYHLEARDTPPWQPQNLERERQAVIRGQAVRKGGTVATLRGASDHREPRGPSRPQSMPLEENEIDREQIDFLAVRQQFLNLEQANPPPGAAHANARVLQSPQSKVAHAKTGVSRNAPLGVAHVNSEVPQSPQPKVAHVNAVASQNSPPRASQASKALSRPPLANGYVLPVKPQVKEVVTEEKRVHPLPTRSVAQALEDPGSRSREESPEPLKETPIEREIRLAQEREADLREQRGLQRATSHQELVEIPARLLLSKVSLAAAPRRDRGRPSLYVQRDIAQESQREEDHRREGLQVGRASTPDWSSEGPQPRLRRVHSSDSILSPTPDAHAASLAPEVRKVSRIPPDAYQPYLSPGTPPGESPASHAYCRPSNLSADEARPVGSPKAAGSQRHPSESSAKPSGLKQEPPRGPLHASRGVLRQEYFHLRPLRFRVPDEPERAEAPRVWGWEVAGAPALRLQKSQSSELLEREVENVLRREREMAEERRSALYPEVFSDECCDHDSRSSSRKSSITGSYSVSESHYFTPIHLHSDLVWTVEAPAKDALQQKKKKEQWYAGINPLDDVNSEILEATRVTRHRNAMAERWEAGIYASEDED

Target ORF information:

RefSeq Version	XM_005209260.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mitotic spindle positioning (MISP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209260.3

ORF Insert Sequence:

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACACCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCCCCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MISP ( XM_005209261.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209318.1 Bos taurus mitotic spindle positioning (MISP), transcript variant X3, mRNA.

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CloneID	OBa21400
Clone ID Related Accession (Same CDS sequence)	XM_005209261.3 , XM_005209259.2 , XM_005209260.4 , XM_005209260.3 , XM_005209261.4
Accession Version	XM_005209261.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mitotic interactor and substrate of PLK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209261.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACACCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCCCCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209318.1
CDS	189..2258
Translation	MDRVTRYPIFGIPHSSRMAGVAFDGDTSYTFELVGVGPTASFWSQDETPAWPTDHEASLEMTRTGTSRSLRVFPGRMAPWPPCPQDNEDEEVKAYHLEARDTPPWQPQNLERERQAVIRGQAVRKGGTVATLRGASDHREPRGPSRPQSMPLEENEIDREQIDFLAVRQQFLNLEQANPPPGAAHANARVLQSPQSKVAHAKTGVSRNAPLGVAHVNSEVPQSPQPKVAHVNAVASQNSPPRASQASKALSRPPLANGYVLPVKPQVKEVVTEEKRVHPLPTRSVAQALEDPGSRSREESPEPLKETPIEREIRLAQEREADLREQRGLQRATSHQELVEIPARLLLSKVSLAAAPRRDRGRPSLYVQRDIAQESQREEDHRREGLQVGRASTPDWSSEGPQPRLRRVHSSDSILSPTPDAHAASLAPEVRKVSRIPPDAYQPYLSPGTPPGESPASHAYCRPSNLSADEARPVGSPKAAGSQRHPSESSAKPSGLKQEPPRGPLHASRGVLRQEYFHLRPLRFRVPDEPERAEAPRVWGWEVAGAPALRLQKSQSSELLEREVENVLRREREMAEERRSALYPEVFSDECCDHDSRSSSRKSSITGSYSVSESHYFTPIHLHSDLVWTVEAPAKDALQQKKKKEQWYAGINPLDDVNSEILEATRVTRHRNAMAERWEAGIYASEDED

Target ORF information:

RefSeq Version	XM_005209261.4
Organism	Bos taurus(cattle)
Definition	Bos taurus mitotic spindle positioning (MISP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209261.4

ORF Insert Sequence:

ATGGACCGAG TTACCAGATA TCCCATCTTC GGCATCCCTC ACTCATCCCG CATGGCCGGT 
GTGGCCTTTG ATGGTGACAC CAGCTACACT TTTGAGTTGG TGGGCGTGGG GCCCACGGCC 
AGTTTCTGGA GCCAGGACGA GACACCAGCA TGGCCCACTG ACCATGAGGC CTCCCTGGAA 
ATGACGCGGA CTGGGACATC CCGCAGCCTG CGTGTCTTCC CTGGCCGGAT GGCCCCATGG 
CCACCCTGCC CCCAGGATAA CGAGGATGAG GAGGTGAAGG CCTACCACCT GGAGGCCAGG 
GACACCCCAC CCTGGCAGCC ACAGAACCTG GAGCGGGAGC GCCAGGCAGT CATTCGGGGC 
CAGGCTGTCA GGAAGGGTGG CACGGTGGCC ACGCTCCGTG GTGCCTCTGA CCACAGGGAG 
CCCAGGGGCC CCAGCCGACC ACAGTCCATG CCCCTGGAGG AGAACGAGAT AGACAGGGAG 
CAGATTGACT TCCTGGCAGT CAGGCAGCAG TTCCTGAACC TGGAGCAGGC GAACCCCCCA 
CCTGGAGCAG CCCACGCCAA TGCAAGGGTT CTCCAGAGCC CCCAATCCAA GGTAGCCCAT 
GCAAAAACAG GGGTCTCTCG AAACGCCCCA CTTGGGGTGG CCCACGTGAA TTCAGAGGTT 
CCTCAGAGCC CCCAACCCAA AGTGGCCCAT GTAAATGCAG TGGCCTCTCA GAACTCCCCA 
CCCAGGGCCA GTCAGGCCTC TAAGGCCCTT AGCAGGCCAC CTCTGGCCAA TGGGTACGTC 
CTCCCAGTCA AACCCCAGGT GAAGGAGGTG GTCACAGAAG AGAAGAGGGT TCACCCTTTG 
CCTACCAGGT CTGTTGCCCA AGCCCTGGAG GACCCCGGTT CCCGGTCCAG AGAAGAGTCC 
CCAGAGCCCC TCAAGGAGAC GCCCATTGAG CGAGAGATCC GACTGGCCCA GGAACGGGAG 
GCAGACCTCC GAGAGCAGAG GGGGCTTCAG CGGGCAACCA GCCACCAGGA GCTGGTGGAG 
ATCCCAGCCA GGCTCCTGCT AAGCAAGGTG AGCCTGGCTG CAGCACCACG GCGGGACAGG 
GGCCGTCCAT CACTCTACGT ACAGCGGGAC ATTGCTCAGG AGTCACAGCG TGAGGAGGAT 
CACCGGCGGG AGGGGCTGCA GGTGGGCCGG GCATCCACAC CTGACTGGTC CTCCGAAGGA 
CCTCAGCCCA GACTTCGAAG AGTCCACAGC TCAGACTCCA TCCTCAGCCC AACCCCAGAT 
GCCCACGCGG CCAGCCTGGC CCCAGAGGTG AGGAAGGTGA GCCGTATCCC ACCTGATGCC 
TACCAACCAT ACTTGAGCCC TGGGACGCCC CCAGGAGAGT CTCCTGCCTC CCACGCCTAC 
TGCAGGCCCA GCAACCTCTC AGCAGATGAA GCCAGGCCAG TGGGCTCTCC CAAGGCTGCA 
GGGTCTCAGA GGCATCCCTC AGAATCCTCT GCAAAGCCCT CAGGCCTGAA GCAGGAGCCC 
CCCCGGGGAC CCCTGCATGC CAGCAGGGGT GTCCTGAGAC AGGAGTACTT CCATCTGCGG 
CCCCTGCGGT TCAGAGTCCC AGATGAACCT GAGAGAGCTG AGGCCCCCCG AGTTTGGGGC 
TGGGAGGTGG CGGGGGCCCC AGCACTGAGG CTACAGAAGT CCCAGTCATC TGAGCTACTG 
GAGAGAGAAG TGGAGAATGT CCTGCGACGG GAGCGGGAGA TGGCTGAGGA GCGGCGGAGT 
GCTCTGTACC CTGAGGTCTT CTCGGATGAG TGCTGTGACC ACGACTCTCG GAGCTCCTCC 
CGCAAATCTA GCATCACAGG CAGCTACTCA GTGTCTGAGT CACACTATTT CACCCCCATC 
CACCTGCACT CGGACCTGGT GTGGACGGTG GAGGCCCCGG CCAAGGATGC TCTCCAGCAG 
AAAAAGAAGA AGGAACAGTG GTATGCCGGC ATCAATCCCT TGGATGATGT CAACTCGGAG 
ATTCTAGAGG CCACACGGGT GACCCGACAC AGGAATGCCA TGGCAGAGCG CTGGGAAGCC 
GGGATCTACG CCAGCGAGGA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MISP cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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