MISP cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MISP
Entrez Gene ID	126353
Full Name	mitotic spindle positioning
Synonyms	C19orf21,MISP1
General protein information	Preferred Names mitotic spindle positioning Names mitotic interactor and substrate of PLK1 caprice mitotic interactor and substrate of Plk1 mitotic spindle positioning protein
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	The protein encoded by this gene is an actin-bundling protein involved in determining cell morphology and mitotic progression. The encoded protein is required for the proper positioning of the mitotic spindle. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Feb 2016].
Disorder MIM:	615289

mRNA and Protein(s)

mRNA	Protein	Name
XM_011527686.2	XP_011525988.1	mitotic interactor and substrate of PLK1 isoform X1
NM_173481.3	NP_775752.1	mitotic interactor and substrate of PLK1
XM_011527686.1	XP_011525988.1	mitotic interactor and substrate of PLK1 isoform X1
XM_011527685.2	XP_011525987.1	mitotic interactor and substrate of PLK1 isoform X1
NM_173481.4	NP_775752.1	mitotic interactor and substrate of PLK1
NM_173481.2	NP_775752.1	mitotic interactor and substrate of PLK1
XM_011527685.1	XP_011525987.1	mitotic interactor and substrate of PLK1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	C28H19ORF21	XP_418218.4
Pan troglodytes (chimpanzee)	MISP	XP_001146667.1
Bos taurus (cattle)	MISP	NP_001030346.1
Xenopus tropicalis (tropical clawed frog)	misp	XP_002937429.1
Homo sapiens (human)	MISP	NP_775752.1
Mus musculus (house mouse)	Misp	NP_084494.1
Rattus norvegicus (Norway rat)	Misp	NP_001102754.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Caprice/MISP is a novel F-actin bundling protein critical for actin-based cytoskeletal reorganizations.
Kumeta M, Gilmore JL, Umeshima H, Ishikawa M, Kitajiri S, Horigome T, Kengaku M, Takeyasu K
Genes to cells : devoted to molecular & cellular mechanisms19(4)338-49(2014 Apr)

The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.
Maier B, Kirsch M, Anderhub S, Zentgraf H, Kr?mer A
Cell cycle (Georgetown, Tex.)12(9)1457-71(2013 May)

MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression.
Zhu M, Settele F, Kotak S, Sanchez-Pulido L, Ehret L, Ponting CP, G?nczy P, Hoffmann I
The Journal of cell biology200(6)773-87(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bundles F-actin and balances stress fiber-filopodial formation
Title: Caprice/MISP is a novel F-actin bundling protein critical for actin-based cytoskeletal reorganizations.

MISP links microtubules to the actin cytoskeleton and focal adhesions in order to properly position the mitotic spindle.
Title: The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.

Plk1 phosphorylates MISP, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning.
Title: MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression.

The following MISP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MISP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17108	NM_173481.4 Latest version!	Homo sapiens mitotic spindle positioning (MISP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu17108	XM_011527686.2 Latest version!	Homo sapiens mitotic spindle positioning (MISP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu17108	XM_011527685.2 Latest version!	Homo sapiens mitotic spindle positioning (MISP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
View Old Accession Versions >>
OHu17108	XM_011527686.1	Homo sapiens mitotic spindle positioning (MISP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu17108	NM_173481.3	Homo sapiens mitotic spindle positioning (MISP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu17108	NM_173481.2	Homo sapiens mitotic spindle positioning (MISP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu17108	XM_011527685.1	Homo sapiens mitotic spindle positioning (MISP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MISP ( NM_173481.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_775752.1 Homo sapiens mitotic spindle positioning (MISP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17108
Clone ID Related Accession (Same CDS sequence)	XM_011527686.2 , NM_173481.3 , XM_011527686.1 , XM_011527685.2 , NM_173481.4 , NM_173481.2 , XM_011527685.1
Accession Version	NM_173481.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	mitotic interactor and substrate of PLK1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY163020.1, BC052236.1 and AW272530.1. On Feb 17, 2016 this sequence version replaced NM_173481.2. Transcript Variant: This variant (1) represents the longer, protein-coding transcript. ##Evidence-Data-START## Transcript exon combination :: BC052236.1, BX648389.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775752.1
CDS	117..2156
Translation	MDRVTRYPILGIPQAHRGTGLVLDGDTSYTYHLVCMGPEASGWGQDEPQTWPTDHRAQQGVQRQGVSYSVHAYTGQPSPRGLHSENREDEGWQVYRLGARDAHQGRPTWALRPEDGEDKEMKTYRLDAGDADPRRLCDLERERWAVIQGQAVRKSSTVATLQGTPDHGDPRTPGPPRSTPLEENVVDREQIDFLAARQQFLSLEQANKGAPHSSPARGTPAGTTPGASQAPKAFNKPHLANGHVVPIKPQVKGVVREENKVRAVPTWASVQVVDDPGSLASVESPGTPKETPIEREIRLAQEREADLREQRGLRQATDHQELVEIPTRPLLTKLSLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRALSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSLSTAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRPLRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERRNALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD

Target ORF information:

RefSeq Version	NM_173481.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitotic spindle positioning (MISP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173481.3

ORF Insert Sequence:

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MISP ( NM_173481.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_775752.1 Homo sapiens mitotic spindle positioning (MISP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17108
Clone ID Related Accession (Same CDS sequence)	XM_011527686.2 , NM_173481.3 , XM_011527686.1 , XM_011527685.2 , NM_173481.4 , NM_173481.2 , XM_011527685.1
Accession Version	NM_173481.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	mitotic interactor and substrate of PLK1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004030.2, HY163020.1 and BC052236.1. On Nov 23, 2018 this sequence version replaced NM_173481.3. Transcript Variant: This variant (1) represents the longer, protein-coding transcript. ##Evidence-Data-START## Transcript exon combination :: BC052236.1, BX648389.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000215582.8/ ENSP00000215582.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775752.1
CDS	118..2157
Translation	MDRVTRYPILGIPQAHRGTGLVLDGDTSYTYHLVCMGPEASGWGQDEPQTWPTDHRAQQGVQRQGVSYSVHAYTGQPSPRGLHSENREDEGWQVYRLGARDAHQGRPTWALRPEDGEDKEMKTYRLDAGDADPRRLCDLERERWAVIQGQAVRKSSTVATLQGTPDHGDPRTPGPPRSTPLEENVVDREQIDFLAARQQFLSLEQANKGAPHSSPARGTPAGTTPGASQAPKAFNKPHLANGHVVPIKPQVKGVVREENKVRAVPTWASVQVVDDPGSLASVESPGTPKETPIEREIRLAQEREADLREQRGLRQATDHQELVEIPTRPLLTKLSLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRALSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSLSTAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRPLRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERRNALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD

Target ORF information:

RefSeq Version	NM_173481.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitotic spindle positioning (MISP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173481.4

ORF Insert Sequence:

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MISP ( NM_173481.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_775752.1 Homo sapiens mitotic spindle positioning (MISP), mRNA.

Price & Availability↑

CloneID	OHu17108
Clone ID Related Accession (Same CDS sequence)	XM_011527686.2 , NM_173481.3 , XM_011527686.1 , XM_011527685.2 , NM_173481.4 , NM_173481.2 , XM_011527685.1
Accession Version	NM_173481.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	mitotic interactor and substrate of PLK1
Comment

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_775752.1

Translation

MDRVTRYPILGIPQAHRGTGLVLDGDTSYTYHLVCMGPEASGWGQDEPQTWPTDHRAQQGVQRQGVSYSVHAYTGQPSPRGLHSENREDEGWQVYRLGARDAHQGRPTWALRPEDGEDKEMKTYRLDAGDADPRRLCDLERERWAVIQGQAVRKSSTVATLQGTPDHGDPRTPGPPRSTPLEENVVDREQIDFLAARQQFLSLEQANKGAPHSSPARGTPAGTTPGASQAPKAFNKPHLANGHVVPIKPQVKGVVREENKVRAVPTWASVQVVDDPGSLASVESPGTPKETPIEREIRLAQEREADLREQRGLRQATDHQELVEIPTRPLLTKLSLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRALSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSLSTAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRPLRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERRNALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD

Target ORF information:

RefSeq Version	NM_173481.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens mitotic spindle positioning (MISP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173481.2

ORF Insert Sequence:

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MISP ( XM_011527686.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011525988.1 Homo sapiens mitotic spindle positioning (MISP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu17108
Clone ID Related Accession (Same CDS sequence)	XM_011527686.2 , NM_173481.3 , XM_011527686.1 , XM_011527685.2 , NM_173481.4 , NM_173481.2 , XM_011527685.1
Accession Version	XM_011527686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitotic interactor and substrate of PLK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525988.1
CDS	255..2294
Translation	MDRVTRYPILGIPQAHRGTGLVLDGDTSYTYHLVCMGPEASGWGQDEPQTWPTDHRAQQGVQRQGVSYSVHAYTGQPSPRGLHSENREDEGWQVYRLGARDAHQGRPTWALRPEDGEDKEMKTYRLDAGDADPRRLCDLERERWAVIQGQAVRKSSTVATLQGTPDHGDPRTPGPPRSTPLEENVVDREQIDFLAARQQFLSLEQANKGAPHSSPARGTPAGTTPGASQAPKAFNKPHLANGHVVPIKPQVKGVVREENKVRAVPTWASVQVVDDPGSLASVESPGTPKETPIEREIRLAQEREADLREQRGLRQATDHQELVEIPTRPLLTKLSLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRALSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSLSTAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRPLRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERRNALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD

Target ORF information:

RefSeq Version	XM_011527686.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitotic spindle positioning (MISP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527686.2

ORF Insert Sequence:

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MISP ( XM_011527686.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011525988.1 Homo sapiens mitotic spindle positioning (MISP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu17108
Clone ID Related Accession (Same CDS sequence)	XM_011527686.2 , NM_173481.3 , XM_011527686.1 , XM_011527685.2 , NM_173481.4 , NM_173481.2 , XM_011527685.1
Accession Version	XM_011527686.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mitotic interactor and substrate of PLK1 isoform X1
Comment

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011525988.1

Translation

MDRVTRYPILGIPQAHRGTGLVLDGDTSYTYHLVCMGPEASGWGQDEPQTWPTDHRAQQGVQRQGVSYSVHAYTGQPSPRGLHSENREDEGWQVYRLGARDAHQGRPTWALRPEDGEDKEMKTYRLDAGDADPRRLCDLERERWAVIQGQAVRKSSTVATLQGTPDHGDPRTPGPPRSTPLEENVVDREQIDFLAARQQFLSLEQANKGAPHSSPARGTPAGTTPGASQAPKAFNKPHLANGHVVPIKPQVKGVVREENKVRAVPTWASVQVVDDPGSLASVESPGTPKETPIEREIRLAQEREADLREQRGLRQATDHQELVEIPTRPLLTKLSLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRALSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSLSTAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRPLRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERRNALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD

Target ORF information:

RefSeq Version	XM_011527686.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mitotic spindle positioning (MISP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527686.1

ORF Insert Sequence:

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MISP ( XM_011527685.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011525987.1 Homo sapiens mitotic spindle positioning (MISP), transcript variant X1, mRNA.

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CloneID	OHu17108
Clone ID Related Accession (Same CDS sequence)	XM_011527686.2 , NM_173481.3 , XM_011527686.1 , XM_011527685.2 , NM_173481.4 , NM_173481.2 , XM_011527685.1
Accession Version	XM_011527685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitotic interactor and substrate of PLK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525987.1
CDS	162..2201
Translation	MDRVTRYPILGIPQAHRGTGLVLDGDTSYTYHLVCMGPEASGWGQDEPQTWPTDHRAQQGVQRQGVSYSVHAYTGQPSPRGLHSENREDEGWQVYRLGARDAHQGRPTWALRPEDGEDKEMKTYRLDAGDADPRRLCDLERERWAVIQGQAVRKSSTVATLQGTPDHGDPRTPGPPRSTPLEENVVDREQIDFLAARQQFLSLEQANKGAPHSSPARGTPAGTTPGASQAPKAFNKPHLANGHVVPIKPQVKGVVREENKVRAVPTWASVQVVDDPGSLASVESPGTPKETPIEREIRLAQEREADLREQRGLRQATDHQELVEIPTRPLLTKLSLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRALSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSLSTAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRPLRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERRNALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD

Target ORF information:

RefSeq Version	XM_011527685.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitotic spindle positioning (MISP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527685.2

ORF Insert Sequence:

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MISP ( XM_011527685.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011525987.1 Homo sapiens mitotic spindle positioning (MISP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu17108
Clone ID Related Accession (Same CDS sequence)	XM_011527686.2 , NM_173481.3 , XM_011527686.1 , XM_011527685.2 , NM_173481.4 , NM_173481.2 , XM_011527685.1
Accession Version	XM_011527685.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mitotic interactor and substrate of PLK1 isoform X1
Comment

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011525987.1

Translation

MDRVTRYPILGIPQAHRGTGLVLDGDTSYTYHLVCMGPEASGWGQDEPQTWPTDHRAQQGVQRQGVSYSVHAYTGQPSPRGLHSENREDEGWQVYRLGARDAHQGRPTWALRPEDGEDKEMKTYRLDAGDADPRRLCDLERERWAVIQGQAVRKSSTVATLQGTPDHGDPRTPGPPRSTPLEENVVDREQIDFLAARQQFLSLEQANKGAPHSSPARGTPAGTTPGASQAPKAFNKPHLANGHVVPIKPQVKGVVREENKVRAVPTWASVQVVDDPGSLASVESPGTPKETPIEREIRLAQEREADLREQRGLRQATDHQELVEIPTRPLLTKLSLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRALSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSLSTAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRPLRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERRNALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD

Target ORF information:

RefSeq Version	XM_011527685.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mitotic spindle positioning (MISP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527685.1

ORF Insert Sequence:

ATGGACCGCG TGACCAGATA CCCCATCCTG GGCATCCCTC AGGCACACCG TGGCACCGGC 
CTGGTGCTGG ATGGAGACAC CAGCTACACA TACCATCTGG TGTGCATGGG CCCCGAGGCC 
AGCGGCTGGG GCCAGGATGA GCCGCAGACA TGGCCCACTG ACCACAGGGC CCAGCAGGGC 
GTGCAGAGGC AGGGGGTGTC CTACAGCGTG CATGCCTACA CTGGCCAGCC GTCCCCACGG 
GGGCTCCACT CGGAGAACAG GGAGGATGAG GGTTGGCAGG TTTACCGCCT GGGCGCCAGG 
GATGCCCACC AGGGACGTCC AACATGGGCA CTCCGCCCAG AGGACGGGGA GGACAAGGAG 
ATGAAGACCT ACCGCCTGGA TGCTGGGGAC GCTGACCCCA GGAGGCTGTG TGACCTGGAG 
CGGGAGCGCT GGGCCGTCAT CCAGGGCCAG GCAGTCAGGA AGAGCAGCAC CGTGGCCACG 
CTCCAGGGCA CTCCTGACCA CGGAGACCCC AGGACCCCCG GCCCACCTCG GTCCACGCCC 
CTGGAGGAGA ACGTGGTTGA CAGGGAGCAG ATTGACTTCC TGGCAGCGAG ACAGCAGTTC 
CTGAGTCTGG AGCAGGCGAA CAAGGGGGCC CCTCATAGCT CCCCGGCCAG GGGGACCCCT 
GCAGGCACAA CCCCAGGGGC CAGCCAGGCC CCCAAGGCCT TCAACAAGCC CCACCTGGCC 
AACGGGCACG TGGTTCCCAT CAAGCCCCAG GTGAAGGGGG TGGTCAGGGA AGAGAACAAG 
GTGCGTGCTG TGCCCACCTG GGCCAGTGTC CAAGTTGTGG ATGACCCTGG CTCCTTGGCC 
TCAGTGGAGT CCCCGGGGAC CCCCAAGGAG ACGCCCATCG AGCGGGAGAT CCGTCTGGCT 
CAGGAGCGTG AGGCAGACCT GCGAGAGCAG AGGGGGCTTC GGCAGGCAAC CGACCACCAG 
GAGCTGGTGG AAATCCCCAC CAGGCCGCTG CTGACCAAGC TGAGCCTGAT CACAGCCCCA 
CGGCGGGAGA GAGGGCGCCC GTCCCTCTAC GTGCAGCGGG ACATAGTACA GGAGACACAG 
CGTGAGGAAG ACCACCGGCG GGAGGGCCTG CACGTGGGCC GGGCGTCCAC ACCCGACTGG 
GTCTCGGAGG GTCCCCAGCC CGGACTCCGG AGAGCCCTCA GCTCAGATTC CATCCTCAGC 
CCGGCCCCAG ATGCCCGTGC GGCCGACCCA GCTCCAGAAG TGAGGAAGGT GAACCGCATC 
CCACCTGATG CCTACCAGCC GTACCTGAGC CCCGGGACCC CCCAGCTAGA ATTCTCAGCC 
TTCGGAGCAT TCGGCAAGCC CAGCAGTCTC TCCACAGCGG AGGCCAAGGC TGCGACTTCA 
CCAAAGGCCA CGATGTCCCC GAGGCATCTC TCAGAATCCT CTGGAAAACC CCTGAGCACA 
AAGCAAGAGG CATCGAAGCC CCCTCGGGGA TGCCCGCAAG CCAACAGGGG TGTCGTGCGG 
TGGGAGTACT TCCGCCTGCG TCCTCTGCGG TTCAGGGCCC CAGACGAGCC CCAGCAGGCC 
CAAGTCCCCC ATGTCTGGGG CTGGGAGGTG GCTGGGGCCC CTGCACTGAG GCTGCAGAAG 
TCCCAGTCAT CTGATCTGCT GGAAAGGGAG AGGGAGAGTG TCCTGCGCCG GGAGCAAGAG 
GTGGCAGAGG AGCGGAGAAA TGCTCTCTTC CCAGAGGTCT TCTCCCCAAC GCCAGATGAG 
AACTCTGACC AGAACTCCAG GAGCTCCTCC CAGGCATCCG GCATCACGGG CAGTTACTCG 
GTGTCTGAGT CTCCCTTCTT CAGCCCCATC CACCTACACT CAAACGTGGC GTGGACAGTG 
GAAGATCCAG TGGACAGTGC TCCTCCCGGG CAGAGAAAGA AGGAGCAATG GTACGCTGGC 
ATCAACCCCT CGGACGGTAT CAACTCAGAG GTCCTGGAAG CCATACGGGT GACCCGTCAC 
AAGAACGCCA TGGCAGAGCG CTGGGAATCC CGCATCTACG CCAGTGAGGA GGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Caprice/MISP is a novel F-actin bundling protein critical for actin-based cytoskeletal reorganizations.
Genes to cells : devoted to molecular & cellular mechanisms19(4)338-49(2014 Apr)
Kumeta M,Gilmore JL,Umeshima H,Ishikawa M,Kitajiri S,Horigome T,Kengaku M,Takeyasu K

The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.
Cell cycle (Georgetown, Tex.)12(9)1457-71(2013 May)
Maier B,Kirsch M,Anderhub S,Zentgraf H,Kr?mer A

MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression.
The Journal of cell biology200(6)773-87(2013 Mar)
Zhu M,Settele F,Kotak S,Sanchez-Pulido L,Ehret L,Ponting CP,G?nczy P,Hoffmann I

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MISP cDNA ORF clone, Homo sapiens(Human)

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gRNAs

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