ECT2 cDNA ORF clone, Bos taurus(cattle)

The following ECT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa40909 NM_001097573.1
Latest version!
Bos taurus epithelial cell transforming 2 (ECT2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa15869 XM_024997071.1
Latest version!
Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa15869 XM_005201670.4
Latest version!
Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46074 XM_010801362.2
Latest version!
Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46075 XM_010801363.2
Latest version!
Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46076 XM_010801365.3
Latest version!
Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46076 XM_010801364.3
Latest version!
Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46076 XM_010801366.2
Latest version!
Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46077 XM_015472466.1
Latest version!
Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa218636 XM_024996990.1
Latest version!
Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa218637 XM_024997128.1
Latest version!
Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OBa46076 XM_010801364.2 Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46076 XM_010801365.2 Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa15869 XM_005201670.3 Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa40909
    Clone ID Related Accession (Same CDS sequence) NM_001097573.1
    Accession Version NM_001097573.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product protein ECT2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC140473.1. On May 24, 2007 this sequence version replaced XM_580911.3. ##Evidence-Data-START## Transcript exon combination :: BC140473.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG CAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGTCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCGAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001091042.1
    CDS97..2841
    Translation

    Target ORF information:

    RefSeq Version NM_001097573.1
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001097573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG CAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGTCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCGAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15869
    Clone ID Related Accession (Same CDS sequence) XM_005201670.3 , XM_005201670.4 , XM_024997071.1
    Accession Version XM_005201670.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG GAAATTAAAC TGCCGTTTGT AAAGATGGAA
    TCAGTTGAAG AATTTAAAAG TTTGGATTCT CCAGAATTTG AAAATGTATT CATAGTCATG
    GACTTCCAGG ATTCTGTCTT TAATGAGTTA TACAAGGCTG ATTGTAGAGT TATTGGACCA
    CCAGTTGTAT TAAACTGTGC AGAAAAAGGA GAGCCTTTGC CATTTTCATG TCGCCCACTC
    TATTGTACCA GCATGATGAA TCTAGTACTA TGTTTTACTG GATTCAGGAA GAAAGAAGAA
    CTAGCCAGAT TGGTGACATT GGTTCATCAC ATGGGTGGAG TTATTCGAAA AGACTTTAAT
    TCAAAAGTTA CACATTTGGT GGCAAATTGT ACACAAGGAG AAAAATTCAG GGTAGCTGTG
    AGCCTGGGTA CTCCAATTAT GAAACCAGAA TGGATTCATA AAGCTTGGGA AAGGCGGAAT
    GAACAGGACT TCTGTGCATC AGTTGATGAC TTTAGAAATG AATTTAAAGT TCCTCCATTT
    CAAGATTGCA TTTTAAGTTT CCTGGGTTTT TCGGATGAAG AGAAAACCAA TATGGAAGAA
    ATGACTAAAA TGCAAGGAGG AAACTGTTTA CCAGTTGGAG ATGAAAGATG CACTCACCTT
    ATAGTTGAAG AGAATATAGT AAAAGAACTT CCATTTGAAC CTTCAAAGAA ACTTTATGTT
    GTGAAACAAG AGTGGTTCTG GGGAAGCATT CAAATGGATG CCCGAGCTGG AGAAACTATG
    TATTTATATG AAAAGGCCAA TACACCTGAG CTCAAGAAAT CAGTGTCACT GCTTTCTCTA
    AATACCCCAA ACAGCAATCG CAAAAGACGT CGTTTAAAAG AGACACTTGC TCAGCTTTCA
    AGAGAGGCAG ACTTGTCACC TTTCCCTCCC CGTAAGCGCC CTTCAGCTGA ACATTCCCTT
    TCTATAGGGT CACTCCTAGA TATCTCCAAC ACACCAGAAT CTAGCATTAA CTATGGAGAA
    ACACCAAAAT CTTGTACTAA GTCCTCTAAG AATTCTACTC CAGTTCCTTC AAAGCAGTCA
    GCCAGGTGGC AAGTTGCAAA AGAGCTCTAT CAGACTGAAA GTAATTATGT AAATATATTG
    GCCACAATTA TTCAGTTATT TCAAGTACCA TTGGAAGAGG AAGGACAACG AGGTGGGCCT
    ATCCTTGCAC CGGAAGAAAT TAAGACGATT TTTGGTAGTA TTCCAGATAT CTTTGATGTG
    CACACTAAGA TAAAGGATGA TCTTGAAGAC CTTATTGTTA ATTGGGATGA GAGCAAAAGC
    ATTGGTGATA TCTTTCTTAA ATATTCCAAA GATTTGGTAA AAATCTATCC TCCCTTTGTG
    AACTTCTTTG AAATGAGCAA GGAAACTATT ATTAAATGTG AAAAACAGAA ACCAAGATTT
    CATGCTTTTC TGAAGATAAA CCAAGCTAAG CCAGAATGTG GACGGCAAAG CCTTGTTGAA
    CTTCTCATCC GACCAGTGCA GAGATTACCC AGCATTGCAT TACTTTTAAA TGATCTTAAG
    AAGCACACAT TTGAAGAAAA TCCTGACAAA AGCACTCTAG AAAAAGCTAT TGAATCACTA
    AAGGAAGTAA TGACCCATAT TAATGAGGAT AAGAGAAAAA CAGAAGCTCA AAGGCAAATT
    TTCGATGTTG TTTATGAGGT AGATGGATGT CCAGCTAATC TTTTATCTTC TCACCGAAGT
    TTAGTTCAGC GAGTTGAAAC AATTTCTCTA GGTGAGCACC CCTGTGACAG AGGAGAACAA
    GTAACTCTCT TCCTCTTCAA TGACTGCCTG GAGATAGCAA GAAAACGACA CAAGGTTATT
    GGTACTTTTA GGAGTCCTCA TGGCCAGACC CGACCCCCAG CTTCTCTTAA GCATATTCAT
    CTAATGCCTC TTTCTCAGAT TAAGAAGGTG CTAGACATAC GAGAGACAGA AGATTGCCAT
    AATGCTTTTG CATTGCTTGT GAGGCCACCA ACAGAGCAAG CAAATGTGCT GCTCAGTTTC
    CAGATGACAT CAGAGGAACT TCCAAAAGAA AACTGGCTAA AGATGCTATG TCGACATGTA
    GCCAACACCA TTTGTAAAGC AGATGCTGAG AATCTTATTT ACACTGCTGA TCCAGAATCC
    TTTGAAGTAA ATACAAAAGA TATGGACAGT ACATTGAGTA GAGCATCTAG AGCAATAAAA
    AAGACTTCAA AAAAGGTTAC CAGAGCATTC TCTTTCTCCA AAACTCCAAA AAGAGCTCTC
    CGAAGGGCTC TTATGGCATC CCAAAGTTCA GCAGAGGGAA GAAGTCCTTC TAGCAATGAG
    AAGCATGTAA TGAGTCGTCT GGCTAGCACA TCATCATTAG CAGGCATCCC GTCTCCCTCC
    CTTGTCAGTC TTCCTGCCTT CTTTGAAAGG AGAAGCCATA CTTTAAGTAG ATCTACAACT
    CATTTGATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201727.1
    CDS180..2831
    Translation

    Target ORF information:

    RefSeq Version XM_005201670.3
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201670.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG GAAATTAAAC TGCCGTTTGT AAAGATGGAA
    TCAGTTGAAG AATTTAAAAG TTTGGATTCT CCAGAATTTG AAAATGTATT CATAGTCATG
    GACTTCCAGG ATTCTGTCTT TAATGAGTTA TACAAGGCTG ATTGTAGAGT TATTGGACCA
    CCAGTTGTAT TAAACTGTGC AGAAAAAGGA GAGCCTTTGC CATTTTCATG TCGCCCACTC
    TATTGTACCA GCATGATGAA TCTAGTACTA TGTTTTACTG GATTCAGGAA GAAAGAAGAA
    CTAGCCAGAT TGGTGACATT GGTTCATCAC ATGGGTGGAG TTATTCGAAA AGACTTTAAT
    TCAAAAGTTA CACATTTGGT GGCAAATTGT ACACAAGGAG AAAAATTCAG GGTAGCTGTG
    AGCCTGGGTA CTCCAATTAT GAAACCAGAA TGGATTCATA AAGCTTGGGA AAGGCGGAAT
    GAACAGGACT TCTGTGCATC AGTTGATGAC TTTAGAAATG AATTTAAAGT TCCTCCATTT
    CAAGATTGCA TTTTAAGTTT CCTGGGTTTT TCGGATGAAG AGAAAACCAA TATGGAAGAA
    ATGACTAAAA TGCAAGGAGG AAACTGTTTA CCAGTTGGAG ATGAAAGATG CACTCACCTT
    ATAGTTGAAG AGAATATAGT AAAAGAACTT CCATTTGAAC CTTCAAAGAA ACTTTATGTT
    GTGAAACAAG AGTGGTTCTG GGGAAGCATT CAAATGGATG CCCGAGCTGG AGAAACTATG
    TATTTATATG AAAAGGCCAA TACACCTGAG CTCAAGAAAT CAGTGTCACT GCTTTCTCTA
    AATACCCCAA ACAGCAATCG CAAAAGACGT CGTTTAAAAG AGACACTTGC TCAGCTTTCA
    AGAGAGGCAG ACTTGTCACC TTTCCCTCCC CGTAAGCGCC CTTCAGCTGA ACATTCCCTT
    TCTATAGGGT CACTCCTAGA TATCTCCAAC ACACCAGAAT CTAGCATTAA CTATGGAGAA
    ACACCAAAAT CTTGTACTAA GTCCTCTAAG AATTCTACTC CAGTTCCTTC AAAGCAGTCA
    GCCAGGTGGC AAGTTGCAAA AGAGCTCTAT CAGACTGAAA GTAATTATGT AAATATATTG
    GCCACAATTA TTCAGTTATT TCAAGTACCA TTGGAAGAGG AAGGACAACG AGGTGGGCCT
    ATCCTTGCAC CGGAAGAAAT TAAGACGATT TTTGGTAGTA TTCCAGATAT CTTTGATGTG
    CACACTAAGA TAAAGGATGA TCTTGAAGAC CTTATTGTTA ATTGGGATGA GAGCAAAAGC
    ATTGGTGATA TCTTTCTTAA ATATTCCAAA GATTTGGTAA AAATCTATCC TCCCTTTGTG
    AACTTCTTTG AAATGAGCAA GGAAACTATT ATTAAATGTG AAAAACAGAA ACCAAGATTT
    CATGCTTTTC TGAAGATAAA CCAAGCTAAG CCAGAATGTG GACGGCAAAG CCTTGTTGAA
    CTTCTCATCC GACCAGTGCA GAGATTACCC AGCATTGCAT TACTTTTAAA TGATCTTAAG
    AAGCACACAT TTGAAGAAAA TCCTGACAAA AGCACTCTAG AAAAAGCTAT TGAATCACTA
    AAGGAAGTAA TGACCCATAT TAATGAGGAT AAGAGAAAAA CAGAAGCTCA AAGGCAAATT
    TTCGATGTTG TTTATGAGGT AGATGGATGT CCAGCTAATC TTTTATCTTC TCACCGAAGT
    TTAGTTCAGC GAGTTGAAAC AATTTCTCTA GGTGAGCACC CCTGTGACAG AGGAGAACAA
    GTAACTCTCT TCCTCTTCAA TGACTGCCTG GAGATAGCAA GAAAACGACA CAAGGTTATT
    GGTACTTTTA GGAGTCCTCA TGGCCAGACC CGACCCCCAG CTTCTCTTAA GCATATTCAT
    CTAATGCCTC TTTCTCAGAT TAAGAAGGTG CTAGACATAC GAGAGACAGA AGATTGCCAT
    AATGCTTTTG CATTGCTTGT GAGGCCACCA ACAGAGCAAG CAAATGTGCT GCTCAGTTTC
    CAGATGACAT CAGAGGAACT TCCAAAAGAA AACTGGCTAA AGATGCTATG TCGACATGTA
    GCCAACACCA TTTGTAAAGC AGATGCTGAG AATCTTATTT ACACTGCTGA TCCAGAATCC
    TTTGAAGTAA ATACAAAAGA TATGGACAGT ACATTGAGTA GAGCATCTAG AGCAATAAAA
    AAGACTTCAA AAAAGGTTAC CAGAGCATTC TCTTTCTCCA AAACTCCAAA AAGAGCTCTC
    CGAAGGGCTC TTATGGCATC CCAAAGTTCA GCAGAGGGAA GAAGTCCTTC TAGCAATGAG
    AAGCATGTAA TGAGTCGTCT GGCTAGCACA TCATCATTAG CAGGCATCCC GTCTCCCTCC
    CTTGTCAGTC TTCCTGCCTT CTTTGAAAGG AGAAGCCATA CTTTAAGTAG ATCTACAACT
    CATTTGATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15869
    Clone ID Related Accession (Same CDS sequence) XM_005201670.3 , XM_005201670.4 , XM_024997071.1
    Accession Version XM_005201670.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201670.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG GAAATTAAAC TGCCGTTTGT AAAGATGGAA
    TCAGTTGAAG AATTTAAAAG TTTGGATTCT CCAGAATTTG AAAATGTATT CATAGTCATG
    GACTTCCAGG ATTCTGTCTT TAATGAGTTA TACAAGGCTG ATTGTAGAGT TATTGGACCA
    CCAGTTGTAT TAAACTGTGC AGAAAAAGGA GAGCCTTTGC CATTTTCATG TCGCCCACTC
    TATTGTACCA GCATGATGAA TCTAGTACTA TGTTTTACTG GATTCAGGAA GAAAGAAGAA
    CTAGCCAGAT TGGTGACATT GGTTCATCAC ATGGGTGGAG TTATTCGAAA AGACTTTAAT
    TCAAAAGTTA CACATTTGGT GGCAAATTGT ACACAAGGAG AAAAATTCAG GGTAGCTGTG
    AGCCTGGGTA CTCCAATTAT GAAACCAGAA TGGATTCATA AAGCTTGGGA AAGGCGGAAT
    GAACAGGACT TCTGTGCATC AGTTGATGAC TTTAGAAATG AATTTAAAGT TCCTCCATTT
    CAAGATTGCA TTTTAAGTTT CCTGGGTTTT TCGGATGAAG AGAAAACCAA TATGGAAGAA
    ATGACTAAAA TGCAAGGAGG AAACTGTTTA CCAGTTGGAG ATGAAAGATG CACTCACCTT
    ATAGTTGAAG AGAATATAGT AAAAGAACTT CCATTTGAAC CTTCAAAGAA ACTTTATGTT
    GTGAAACAAG AGTGGTTCTG GGGAAGCATT CAAATGGATG CCCGAGCTGG AGAAACTATG
    TATTTATATG AAAAGGCCAA TACACCTGAG CTCAAGAAAT CAGTGTCACT GCTTTCTCTA
    AATACCCCAA ACAGCAATCG CAAAAGACGT CGTTTAAAAG AGACACTTGC TCAGCTTTCA
    AGAGAGGCAG ACTTGTCACC TTTCCCTCCC CGTAAGCGCC CTTCAGCTGA ACATTCCCTT
    TCTATAGGGT CACTCCTAGA TATCTCCAAC ACACCAGAAT CTAGCATTAA CTATGGAGAA
    ACACCAAAAT CTTGTACTAA GTCCTCTAAG AATTCTACTC CAGTTCCTTC AAAGCAGTCA
    GCCAGGTGGC AAGTTGCAAA AGAGCTCTAT CAGACTGAAA GTAATTATGT AAATATATTG
    GCCACAATTA TTCAGTTATT TCAAGTACCA TTGGAAGAGG AAGGACAACG AGGTGGGCCT
    ATCCTTGCAC CGGAAGAAAT TAAGACGATT TTTGGTAGTA TTCCAGATAT CTTTGATGTG
    CACACTAAGA TAAAGGATGA TCTTGAAGAC CTTATTGTTA ATTGGGATGA GAGCAAAAGC
    ATTGGTGATA TCTTTCTTAA ATATTCCAAA GATTTGGTAA AAATCTATCC TCCCTTTGTG
    AACTTCTTTG AAATGAGCAA GGAAACTATT ATTAAATGTG AAAAACAGAA ACCAAGATTT
    CATGCTTTTC TGAAGATAAA CCAAGCTAAG CCAGAATGTG GACGGCAAAG CCTTGTTGAA
    CTTCTCATCC GACCAGTGCA GAGATTACCC AGCATTGCAT TACTTTTAAA TGATCTTAAG
    AAGCACACAT TTGAAGAAAA TCCTGACAAA AGCACTCTAG AAAAAGCTAT TGAATCACTA
    AAGGAAGTAA TGACCCATAT TAATGAGGAT AAGAGAAAAA CAGAAGCTCA AAGGCAAATT
    TTCGATGTTG TTTATGAGGT AGATGGATGT CCAGCTAATC TTTTATCTTC TCACCGAAGT
    TTAGTTCAGC GAGTTGAAAC AATTTCTCTA GGTGAGCACC CCTGTGACAG AGGAGAACAA
    GTAACTCTCT TCCTCTTCAA TGACTGCCTG GAGATAGCAA GAAAACGACA CAAGGTTATT
    GGTACTTTTA GGAGTCCTCA TGGCCAGACC CGACCCCCAG CTTCTCTTAA GCATATTCAT
    CTAATGCCTC TTTCTCAGAT TAAGAAGGTG CTAGACATAC GAGAGACAGA AGATTGCCAT
    AATGCTTTTG CATTGCTTGT GAGGCCACCA ACAGAGCAAG CAAATGTGCT GCTCAGTTTC
    CAGATGACAT CAGAGGAACT TCCAAAAGAA AACTGGCTAA AGATGCTATG TCGACATGTA
    GCCAACACCA TTTGTAAAGC AGATGCTGAG AATCTTATTT ACACTGCTGA TCCAGAATCC
    TTTGAAGTAA ATACAAAAGA TATGGACAGT ACATTGAGTA GAGCATCTAG AGCAATAAAA
    AAGACTTCAA AAAAGGTTAC CAGAGCATTC TCTTTCTCCA AAACTCCAAA AAGAGCTCTC
    CGAAGGGCTC TTATGGCATC CCAAAGTTCA GCAGAGGGAA GAAGTCCTTC TAGCAATGAG
    AAGCATGTAA TGAGTCGTCT GGCTAGCACA TCATCATTAG CAGGCATCCC GTCTCCCTCC
    CTTGTCAGTC TTCCTGCCTT CTTTGAAAGG AGAAGCCATA CTTTAAGTAG ATCTACAACT
    CATTTGATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201727.1
    CDS160..2811
    Translation

    Target ORF information:

    RefSeq Version XM_005201670.4
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201670.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG GAAATTAAAC TGCCGTTTGT AAAGATGGAA
    TCAGTTGAAG AATTTAAAAG TTTGGATTCT CCAGAATTTG AAAATGTATT CATAGTCATG
    GACTTCCAGG ATTCTGTCTT TAATGAGTTA TACAAGGCTG ATTGTAGAGT TATTGGACCA
    CCAGTTGTAT TAAACTGTGC AGAAAAAGGA GAGCCTTTGC CATTTTCATG TCGCCCACTC
    TATTGTACCA GCATGATGAA TCTAGTACTA TGTTTTACTG GATTCAGGAA GAAAGAAGAA
    CTAGCCAGAT TGGTGACATT GGTTCATCAC ATGGGTGGAG TTATTCGAAA AGACTTTAAT
    TCAAAAGTTA CACATTTGGT GGCAAATTGT ACACAAGGAG AAAAATTCAG GGTAGCTGTG
    AGCCTGGGTA CTCCAATTAT GAAACCAGAA TGGATTCATA AAGCTTGGGA AAGGCGGAAT
    GAACAGGACT TCTGTGCATC AGTTGATGAC TTTAGAAATG AATTTAAAGT TCCTCCATTT
    CAAGATTGCA TTTTAAGTTT CCTGGGTTTT TCGGATGAAG AGAAAACCAA TATGGAAGAA
    ATGACTAAAA TGCAAGGAGG AAACTGTTTA CCAGTTGGAG ATGAAAGATG CACTCACCTT
    ATAGTTGAAG AGAATATAGT AAAAGAACTT CCATTTGAAC CTTCAAAGAA ACTTTATGTT
    GTGAAACAAG AGTGGTTCTG GGGAAGCATT CAAATGGATG CCCGAGCTGG AGAAACTATG
    TATTTATATG AAAAGGCCAA TACACCTGAG CTCAAGAAAT CAGTGTCACT GCTTTCTCTA
    AATACCCCAA ACAGCAATCG CAAAAGACGT CGTTTAAAAG AGACACTTGC TCAGCTTTCA
    AGAGAGGCAG ACTTGTCACC TTTCCCTCCC CGTAAGCGCC CTTCAGCTGA ACATTCCCTT
    TCTATAGGGT CACTCCTAGA TATCTCCAAC ACACCAGAAT CTAGCATTAA CTATGGAGAA
    ACACCAAAAT CTTGTACTAA GTCCTCTAAG AATTCTACTC CAGTTCCTTC AAAGCAGTCA
    GCCAGGTGGC AAGTTGCAAA AGAGCTCTAT CAGACTGAAA GTAATTATGT AAATATATTG
    GCCACAATTA TTCAGTTATT TCAAGTACCA TTGGAAGAGG AAGGACAACG AGGTGGGCCT
    ATCCTTGCAC CGGAAGAAAT TAAGACGATT TTTGGTAGTA TTCCAGATAT CTTTGATGTG
    CACACTAAGA TAAAGGATGA TCTTGAAGAC CTTATTGTTA ATTGGGATGA GAGCAAAAGC
    ATTGGTGATA TCTTTCTTAA ATATTCCAAA GATTTGGTAA AAATCTATCC TCCCTTTGTG
    AACTTCTTTG AAATGAGCAA GGAAACTATT ATTAAATGTG AAAAACAGAA ACCAAGATTT
    CATGCTTTTC TGAAGATAAA CCAAGCTAAG CCAGAATGTG GACGGCAAAG CCTTGTTGAA
    CTTCTCATCC GACCAGTGCA GAGATTACCC AGCATTGCAT TACTTTTAAA TGATCTTAAG
    AAGCACACAT TTGAAGAAAA TCCTGACAAA AGCACTCTAG AAAAAGCTAT TGAATCACTA
    AAGGAAGTAA TGACCCATAT TAATGAGGAT AAGAGAAAAA CAGAAGCTCA AAGGCAAATT
    TTCGATGTTG TTTATGAGGT AGATGGATGT CCAGCTAATC TTTTATCTTC TCACCGAAGT
    TTAGTTCAGC GAGTTGAAAC AATTTCTCTA GGTGAGCACC CCTGTGACAG AGGAGAACAA
    GTAACTCTCT TCCTCTTCAA TGACTGCCTG GAGATAGCAA GAAAACGACA CAAGGTTATT
    GGTACTTTTA GGAGTCCTCA TGGCCAGACC CGACCCCCAG CTTCTCTTAA GCATATTCAT
    CTAATGCCTC TTTCTCAGAT TAAGAAGGTG CTAGACATAC GAGAGACAGA AGATTGCCAT
    AATGCTTTTG CATTGCTTGT GAGGCCACCA ACAGAGCAAG CAAATGTGCT GCTCAGTTTC
    CAGATGACAT CAGAGGAACT TCCAAAAGAA AACTGGCTAA AGATGCTATG TCGACATGTA
    GCCAACACCA TTTGTAAAGC AGATGCTGAG AATCTTATTT ACACTGCTGA TCCAGAATCC
    TTTGAAGTAA ATACAAAAGA TATGGACAGT ACATTGAGTA GAGCATCTAG AGCAATAAAA
    AAGACTTCAA AAAAGGTTAC CAGAGCATTC TCTTTCTCCA AAACTCCAAA AAGAGCTCTC
    CGAAGGGCTC TTATGGCATC CCAAAGTTCA GCAGAGGGAA GAAGTCCTTC TAGCAATGAG
    AAGCATGTAA TGAGTCGTCT GGCTAGCACA TCATCATTAG CAGGCATCCC GTCTCCCTCC
    CTTGTCAGTC TTCCTGCCTT CTTTGAAAGG AGAAGCCATA CTTTAAGTAG ATCTACAACT
    CATTTGATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15869
    Clone ID Related Accession (Same CDS sequence) XM_005201670.3 , XM_005201670.4 , XM_024997071.1
    Accession Version XM_024997071.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG GAAATTAAAC TGCCGTTTGT AAAGATGGAA
    TCAGTTGAAG AATTTAAAAG TTTGGATTCT CCAGAATTTG AAAATGTATT CATAGTCATG
    GACTTCCAGG ATTCTGTCTT TAATGAGTTA TACAAGGCTG ATTGTAGAGT TATTGGACCA
    CCAGTTGTAT TAAACTGTGC AGAAAAAGGA GAGCCTTTGC CATTTTCATG TCGCCCACTC
    TATTGTACCA GCATGATGAA TCTAGTACTA TGTTTTACTG GATTCAGGAA GAAAGAAGAA
    CTAGCCAGAT TGGTGACATT GGTTCATCAC ATGGGTGGAG TTATTCGAAA AGACTTTAAT
    TCAAAAGTTA CACATTTGGT GGCAAATTGT ACACAAGGAG AAAAATTCAG GGTAGCTGTG
    AGCCTGGGTA CTCCAATTAT GAAACCAGAA TGGATTCATA AAGCTTGGGA AAGGCGGAAT
    GAACAGGACT TCTGTGCATC AGTTGATGAC TTTAGAAATG AATTTAAAGT TCCTCCATTT
    CAAGATTGCA TTTTAAGTTT CCTGGGTTTT TCGGATGAAG AGAAAACCAA TATGGAAGAA
    ATGACTAAAA TGCAAGGAGG AAACTGTTTA CCAGTTGGAG ATGAAAGATG CACTCACCTT
    ATAGTTGAAG AGAATATAGT AAAAGAACTT CCATTTGAAC CTTCAAAGAA ACTTTATGTT
    GTGAAACAAG AGTGGTTCTG GGGAAGCATT CAAATGGATG CCCGAGCTGG AGAAACTATG
    TATTTATATG AAAAGGCCAA TACACCTGAG CTCAAGAAAT CAGTGTCACT GCTTTCTCTA
    AATACCCCAA ACAGCAATCG CAAAAGACGT CGTTTAAAAG AGACACTTGC TCAGCTTTCA
    AGAGAGGCAG ACTTGTCACC TTTCCCTCCC CGTAAGCGCC CTTCAGCTGA ACATTCCCTT
    TCTATAGGGT CACTCCTAGA TATCTCCAAC ACACCAGAAT CTAGCATTAA CTATGGAGAA
    ACACCAAAAT CTTGTACTAA GTCCTCTAAG AATTCTACTC CAGTTCCTTC AAAGCAGTCA
    GCCAGGTGGC AAGTTGCAAA AGAGCTCTAT CAGACTGAAA GTAATTATGT AAATATATTG
    GCCACAATTA TTCAGTTATT TCAAGTACCA TTGGAAGAGG AAGGACAACG AGGTGGGCCT
    ATCCTTGCAC CGGAAGAAAT TAAGACGATT TTTGGTAGTA TTCCAGATAT CTTTGATGTG
    CACACTAAGA TAAAGGATGA TCTTGAAGAC CTTATTGTTA ATTGGGATGA GAGCAAAAGC
    ATTGGTGATA TCTTTCTTAA ATATTCCAAA GATTTGGTAA AAATCTATCC TCCCTTTGTG
    AACTTCTTTG AAATGAGCAA GGAAACTATT ATTAAATGTG AAAAACAGAA ACCAAGATTT
    CATGCTTTTC TGAAGATAAA CCAAGCTAAG CCAGAATGTG GACGGCAAAG CCTTGTTGAA
    CTTCTCATCC GACCAGTGCA GAGATTACCC AGCATTGCAT TACTTTTAAA TGATCTTAAG
    AAGCACACAT TTGAAGAAAA TCCTGACAAA AGCACTCTAG AAAAAGCTAT TGAATCACTA
    AAGGAAGTAA TGACCCATAT TAATGAGGAT AAGAGAAAAA CAGAAGCTCA AAGGCAAATT
    TTCGATGTTG TTTATGAGGT AGATGGATGT CCAGCTAATC TTTTATCTTC TCACCGAAGT
    TTAGTTCAGC GAGTTGAAAC AATTTCTCTA GGTGAGCACC CCTGTGACAG AGGAGAACAA
    GTAACTCTCT TCCTCTTCAA TGACTGCCTG GAGATAGCAA GAAAACGACA CAAGGTTATT
    GGTACTTTTA GGAGTCCTCA TGGCCAGACC CGACCCCCAG CTTCTCTTAA GCATATTCAT
    CTAATGCCTC TTTCTCAGAT TAAGAAGGTG CTAGACATAC GAGAGACAGA AGATTGCCAT
    AATGCTTTTG CATTGCTTGT GAGGCCACCA ACAGAGCAAG CAAATGTGCT GCTCAGTTTC
    CAGATGACAT CAGAGGAACT TCCAAAAGAA AACTGGCTAA AGATGCTATG TCGACATGTA
    GCCAACACCA TTTGTAAAGC AGATGCTGAG AATCTTATTT ACACTGCTGA TCCAGAATCC
    TTTGAAGTAA ATACAAAAGA TATGGACAGT ACATTGAGTA GAGCATCTAG AGCAATAAAA
    AAGACTTCAA AAAAGGTTAC CAGAGCATTC TCTTTCTCCA AAACTCCAAA AAGAGCTCTC
    CGAAGGGCTC TTATGGCATC CCAAAGTTCA GCAGAGGGAA GAAGTCCTTC TAGCAATGAG
    AAGCATGTAA TGAGTCGTCT GGCTAGCACA TCATCATTAG CAGGCATCCC GTCTCCCTCC
    CTTGTCAGTC TTCCTGCCTT CTTTGAAAGG AGAAGCCATA CTTTAAGTAG ATCTACAACT
    CATTTGATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852839.1
    CDS159..2810
    Translation

    Target ORF information:

    RefSeq Version XM_024997071.1
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997071.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG GAAATTAAAC TGCCGTTTGT AAAGATGGAA
    TCAGTTGAAG AATTTAAAAG TTTGGATTCT CCAGAATTTG AAAATGTATT CATAGTCATG
    GACTTCCAGG ATTCTGTCTT TAATGAGTTA TACAAGGCTG ATTGTAGAGT TATTGGACCA
    CCAGTTGTAT TAAACTGTGC AGAAAAAGGA GAGCCTTTGC CATTTTCATG TCGCCCACTC
    TATTGTACCA GCATGATGAA TCTAGTACTA TGTTTTACTG GATTCAGGAA GAAAGAAGAA
    CTAGCCAGAT TGGTGACATT GGTTCATCAC ATGGGTGGAG TTATTCGAAA AGACTTTAAT
    TCAAAAGTTA CACATTTGGT GGCAAATTGT ACACAAGGAG AAAAATTCAG GGTAGCTGTG
    AGCCTGGGTA CTCCAATTAT GAAACCAGAA TGGATTCATA AAGCTTGGGA AAGGCGGAAT
    GAACAGGACT TCTGTGCATC AGTTGATGAC TTTAGAAATG AATTTAAAGT TCCTCCATTT
    CAAGATTGCA TTTTAAGTTT CCTGGGTTTT TCGGATGAAG AGAAAACCAA TATGGAAGAA
    ATGACTAAAA TGCAAGGAGG AAACTGTTTA CCAGTTGGAG ATGAAAGATG CACTCACCTT
    ATAGTTGAAG AGAATATAGT AAAAGAACTT CCATTTGAAC CTTCAAAGAA ACTTTATGTT
    GTGAAACAAG AGTGGTTCTG GGGAAGCATT CAAATGGATG CCCGAGCTGG AGAAACTATG
    TATTTATATG AAAAGGCCAA TACACCTGAG CTCAAGAAAT CAGTGTCACT GCTTTCTCTA
    AATACCCCAA ACAGCAATCG CAAAAGACGT CGTTTAAAAG AGACACTTGC TCAGCTTTCA
    AGAGAGGCAG ACTTGTCACC TTTCCCTCCC CGTAAGCGCC CTTCAGCTGA ACATTCCCTT
    TCTATAGGGT CACTCCTAGA TATCTCCAAC ACACCAGAAT CTAGCATTAA CTATGGAGAA
    ACACCAAAAT CTTGTACTAA GTCCTCTAAG AATTCTACTC CAGTTCCTTC AAAGCAGTCA
    GCCAGGTGGC AAGTTGCAAA AGAGCTCTAT CAGACTGAAA GTAATTATGT AAATATATTG
    GCCACAATTA TTCAGTTATT TCAAGTACCA TTGGAAGAGG AAGGACAACG AGGTGGGCCT
    ATCCTTGCAC CGGAAGAAAT TAAGACGATT TTTGGTAGTA TTCCAGATAT CTTTGATGTG
    CACACTAAGA TAAAGGATGA TCTTGAAGAC CTTATTGTTA ATTGGGATGA GAGCAAAAGC
    ATTGGTGATA TCTTTCTTAA ATATTCCAAA GATTTGGTAA AAATCTATCC TCCCTTTGTG
    AACTTCTTTG AAATGAGCAA GGAAACTATT ATTAAATGTG AAAAACAGAA ACCAAGATTT
    CATGCTTTTC TGAAGATAAA CCAAGCTAAG CCAGAATGTG GACGGCAAAG CCTTGTTGAA
    CTTCTCATCC GACCAGTGCA GAGATTACCC AGCATTGCAT TACTTTTAAA TGATCTTAAG
    AAGCACACAT TTGAAGAAAA TCCTGACAAA AGCACTCTAG AAAAAGCTAT TGAATCACTA
    AAGGAAGTAA TGACCCATAT TAATGAGGAT AAGAGAAAAA CAGAAGCTCA AAGGCAAATT
    TTCGATGTTG TTTATGAGGT AGATGGATGT CCAGCTAATC TTTTATCTTC TCACCGAAGT
    TTAGTTCAGC GAGTTGAAAC AATTTCTCTA GGTGAGCACC CCTGTGACAG AGGAGAACAA
    GTAACTCTCT TCCTCTTCAA TGACTGCCTG GAGATAGCAA GAAAACGACA CAAGGTTATT
    GGTACTTTTA GGAGTCCTCA TGGCCAGACC CGACCCCCAG CTTCTCTTAA GCATATTCAT
    CTAATGCCTC TTTCTCAGAT TAAGAAGGTG CTAGACATAC GAGAGACAGA AGATTGCCAT
    AATGCTTTTG CATTGCTTGT GAGGCCACCA ACAGAGCAAG CAAATGTGCT GCTCAGTTTC
    CAGATGACAT CAGAGGAACT TCCAAAAGAA AACTGGCTAA AGATGCTATG TCGACATGTA
    GCCAACACCA TTTGTAAAGC AGATGCTGAG AATCTTATTT ACACTGCTGA TCCAGAATCC
    TTTGAAGTAA ATACAAAAGA TATGGACAGT ACATTGAGTA GAGCATCTAG AGCAATAAAA
    AAGACTTCAA AAAAGGTTAC CAGAGCATTC TCTTTCTCCA AAACTCCAAA AAGAGCTCTC
    CGAAGGGCTC TTATGGCATC CCAAAGTTCA GCAGAGGGAA GAAGTCCTTC TAGCAATGAG
    AAGCATGTAA TGAGTCGTCT GGCTAGCACA TCATCATTAG CAGGCATCCC GTCTCCCTCC
    CTTGTCAGTC TTCCTGCCTT CTTTGAAAGG AGAAGCCATA CTTTAAGTAG ATCTACAACT
    CATTTGATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46074
    Clone ID Related Accession (Same CDS sequence) XM_010801362.2
    Accession Version XM_010801362.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2835bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTGGATCG TGACCTTTGA AGAAACTGGT GATAGAGACA GTTGTCTGGA ACTGTTGTTT 
    TTCACCAACC ATCATAGAAG AGAACAAACA ATGGCTGATG ATAGTGCATT AGCATCCACT
    ACTGGCAGTA CAAGCTTGGC AGACTCTTCC ATTTTTGATT CTAAAGTTGC CGAAACTTCT
    AAGGAAAACT TATTTATTAG ATCTACTTCA TGTGTTGAAG AAGAAATGCC TCAGATTGAA
    ACAAGAGTCA TACTGGTTCA AGAATCTGGA AAACAAGAAG AACTTATAAG AGCCTTAAAG
    ACTATTAACA TAATGGAAGT CCCTGTTATA AAGATAAAAG AAAGTTGTCC TGGAAAATCG
    GATGAAAAAT TAATAAAAAG TATTGTTAAT ATGGAAATTA AACTGCCGTT TGTAAAGATG
    GAATCAGTTG AAGAATTTAA AAGTTTGGAT TCTCCAGAAT TTGAAAATGT ATTCATAGTC
    ATGGACTTCC AGGATTCTGT CTTTAATGAG TTATACAAGG CTGATTGTAG AGTTATTGGA
    CCACCAGTTG TATTAAACTG TGCAGAAAAA GGAGAGCCTT TGCCATTTTC ATGTCGCCCA
    CTCTATTGTA CCAGCATGAT GAATCTAGTA CTATGTTTTA CTGGATTCAG GAAGAAAGAA
    GAACTAGCCA GATTGGTGAC ATTGGTTCAT CACATGGGTG GAGTTATTCG AAAAGACTTT
    AATTCAAAAG TTACACATTT GGTGGCAAAT TGTACACAAG GAGAAAAATT CAGGGTAGCT
    GTGAGCCTGG GTACTCCAAT TATGAAACCA GAATGGATTC ATAAAGCTTG GGAAAGGCGG
    AATGAACAGG ACTTCTGTGC ATCAGTTGAT GACTTTAGAA ATGAATTTAA AGTTCCTCCA
    TTTCAAGATT GCATTTTAAG TTTCCTGGGT TTTTCGGATG AAGAGAAAAC CAATATGGAA
    GAAATGACTA AAATGCAAGG AGGAAACTGT TTACCAGTTG GAGATGAAAG ATGCACTCAC
    CTTATAGTTG AAGAGAATAT AGTAAAAGAA CTTCCATTTG AACCTTCAAA GAAACTTTAT
    GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC ATTCAAATGG ATGCCCGAGC TGGAGAAACT
    ATGTATTTAT ATGAAAAGGC CAATACACCT GAGCTCAAGA AATCAGTGTC ACTGCTTTCT
    CTAAATACCC CAAACAGCAA TCGCAAAAGA CGTCGTTTAA AAGAGACACT TGCTCAGCTT
    TCAAGAGAGG CAGACTTGTC ACCTTTCCCT CCCCGTAAGC GCCCTTCAGC TGAACATTCC
    CTTTCTATAG GGTCACTCCT AGATATCTCC AACACACCAG AATCTAGCAT TAACTATGGA
    GAAACACCAA AATCTTGTAC TAAGTCCTCT AAGAATTCTA CTCCAGTTCC TTCAAAGCAG
    TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC TATCAGACTG AAAGTAATTA TGTAAATATA
    TTGGCCACAA TTATTCAGTT ATTTCAAGTA CCATTGGAAG AGGAAGGACA ACGAGGTGGG
    CCTATCCTTG CACCGGAAGA AATTAAGACG ATTTTTGGTA GTATTCCAGA TATCTTTGAT
    GTGCACACTA AGATAAAGGA TGATCTTGAA GACCTTATTG TTAATTGGGA TGAGAGCAAA
    AGCATTGGTG ATATCTTTCT TAAATATTCC AAAGATTTGG TAAAAATCTA TCCTCCCTTT
    GTGAACTTCT TTGAAATGAG CAAGGAAACT ATTATTAAAT GTGAAAAACA GAAACCAAGA
    TTTCATGCTT TTCTGAAGAT AAACCAAGCT AAGCCAGAAT GTGGACGGCA AAGCCTTGTT
    GAACTTCTCA TCCGACCAGT GCAGAGATTA CCCAGCATTG CATTACTTTT AAATGATCTT
    AAGAAGCACA CATTTGAAGA AAATCCTGAC AAAAGCACTC TAGAAAAAGC TATTGAATCA
    CTAAAGGAAG TAATGACCCA TATTAATGAG GATAAGAGAA AAACAGAAGC TCAAAGGCAA
    ATTTTCGATG TTGTTTATGA GGTAGATGGA TGTCCAGCTA ATCTTTTATC TTCTCACCGA
    AGTTTAGTTC AGCGAGTTGA AACAATTTCT CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA
    CAAGTAACTC TCTTCCTCTT CAATGACTGC CTGGAGATAG CAAGAAAACG ACACAAGGTT
    ATTGGTACTT TTAGGAGTCC TCATGGCCAG ACCCGACCCC CAGCTTCTCT TAAGCATATT
    CATCTAATGC CTCTTTCTCA GATTAAGAAG GTGCTAGACA TACGAGAGAC AGAAGATTGC
    CATAATGCTT TTGCATTGCT TGTGAGGCCA CCAACAGAGC AAGCAAATGT GCTGCTCAGT
    TTCCAGATGA CATCAGAGGA ACTTCCAAAA GAAAACTGGC TAAAGATGCT ATGTCGACAT
    GTAGCCAACA CCATTTGTAA AGCAGATGCT GAGAATCTTA TTTACACTGC TGATCCAGAA
    TCCTTTGAAG TAAATACAAA AGATATGGAC AGTACATTGA GTAGAGCATC TAGAGCAATA
    AAAAAGACTT CAAAAAAGGT TACCAGAGCA TTCTCTTTCT CCAAAACTCC AAAAAGAGCT
    CTCCGAAGGG CTCTTATGGC ATCCCAAAGT TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT
    GAGAAGCATG TAATGAGTCG TCTGGCTAGC ACATCATCAT TAGCAGGCAT CCCGTCTCCC
    TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA AGGAGAAGCC ATACTTTAAG TAGATCTACA
    ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799664.1
    CDS176..3010
    Translation

    Target ORF information:

    RefSeq Version XM_010801362.2
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801362.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTGGATCG TGACCTTTGA AGAAACTGGT GATAGAGACA GTTGTCTGGA ACTGTTGTTT 
    TTCACCAACC ATCATAGAAG AGAACAAACA ATGGCTGATG ATAGTGCATT AGCATCCACT
    ACTGGCAGTA CAAGCTTGGC AGACTCTTCC ATTTTTGATT CTAAAGTTGC CGAAACTTCT
    AAGGAAAACT TATTTATTAG ATCTACTTCA TGTGTTGAAG AAGAAATGCC TCAGATTGAA
    ACAAGAGTCA TACTGGTTCA AGAATCTGGA AAACAAGAAG AACTTATAAG AGCCTTAAAG
    ACTATTAACA TAATGGAAGT CCCTGTTATA AAGATAAAAG AAAGTTGTCC TGGAAAATCG
    GATGAAAAAT TAATAAAAAG TATTGTTAAT ATGGAAATTA AACTGCCGTT TGTAAAGATG
    GAATCAGTTG AAGAATTTAA AAGTTTGGAT TCTCCAGAAT TTGAAAATGT ATTCATAGTC
    ATGGACTTCC AGGATTCTGT CTTTAATGAG TTATACAAGG CTGATTGTAG AGTTATTGGA
    CCACCAGTTG TATTAAACTG TGCAGAAAAA GGAGAGCCTT TGCCATTTTC ATGTCGCCCA
    CTCTATTGTA CCAGCATGAT GAATCTAGTA CTATGTTTTA CTGGATTCAG GAAGAAAGAA
    GAACTAGCCA GATTGGTGAC ATTGGTTCAT CACATGGGTG GAGTTATTCG AAAAGACTTT
    AATTCAAAAG TTACACATTT GGTGGCAAAT TGTACACAAG GAGAAAAATT CAGGGTAGCT
    GTGAGCCTGG GTACTCCAAT TATGAAACCA GAATGGATTC ATAAAGCTTG GGAAAGGCGG
    AATGAACAGG ACTTCTGTGC ATCAGTTGAT GACTTTAGAA ATGAATTTAA AGTTCCTCCA
    TTTCAAGATT GCATTTTAAG TTTCCTGGGT TTTTCGGATG AAGAGAAAAC CAATATGGAA
    GAAATGACTA AAATGCAAGG AGGAAACTGT TTACCAGTTG GAGATGAAAG ATGCACTCAC
    CTTATAGTTG AAGAGAATAT AGTAAAAGAA CTTCCATTTG AACCTTCAAA GAAACTTTAT
    GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC ATTCAAATGG ATGCCCGAGC TGGAGAAACT
    ATGTATTTAT ATGAAAAGGC CAATACACCT GAGCTCAAGA AATCAGTGTC ACTGCTTTCT
    CTAAATACCC CAAACAGCAA TCGCAAAAGA CGTCGTTTAA AAGAGACACT TGCTCAGCTT
    TCAAGAGAGG CAGACTTGTC ACCTTTCCCT CCCCGTAAGC GCCCTTCAGC TGAACATTCC
    CTTTCTATAG GGTCACTCCT AGATATCTCC AACACACCAG AATCTAGCAT TAACTATGGA
    GAAACACCAA AATCTTGTAC TAAGTCCTCT AAGAATTCTA CTCCAGTTCC TTCAAAGCAG
    TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC TATCAGACTG AAAGTAATTA TGTAAATATA
    TTGGCCACAA TTATTCAGTT ATTTCAAGTA CCATTGGAAG AGGAAGGACA ACGAGGTGGG
    CCTATCCTTG CACCGGAAGA AATTAAGACG ATTTTTGGTA GTATTCCAGA TATCTTTGAT
    GTGCACACTA AGATAAAGGA TGATCTTGAA GACCTTATTG TTAATTGGGA TGAGAGCAAA
    AGCATTGGTG ATATCTTTCT TAAATATTCC AAAGATTTGG TAAAAATCTA TCCTCCCTTT
    GTGAACTTCT TTGAAATGAG CAAGGAAACT ATTATTAAAT GTGAAAAACA GAAACCAAGA
    TTTCATGCTT TTCTGAAGAT AAACCAAGCT AAGCCAGAAT GTGGACGGCA AAGCCTTGTT
    GAACTTCTCA TCCGACCAGT GCAGAGATTA CCCAGCATTG CATTACTTTT AAATGATCTT
    AAGAAGCACA CATTTGAAGA AAATCCTGAC AAAAGCACTC TAGAAAAAGC TATTGAATCA
    CTAAAGGAAG TAATGACCCA TATTAATGAG GATAAGAGAA AAACAGAAGC TCAAAGGCAA
    ATTTTCGATG TTGTTTATGA GGTAGATGGA TGTCCAGCTA ATCTTTTATC TTCTCACCGA
    AGTTTAGTTC AGCGAGTTGA AACAATTTCT CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA
    CAAGTAACTC TCTTCCTCTT CAATGACTGC CTGGAGATAG CAAGAAAACG ACACAAGGTT
    ATTGGTACTT TTAGGAGTCC TCATGGCCAG ACCCGACCCC CAGCTTCTCT TAAGCATATT
    CATCTAATGC CTCTTTCTCA GATTAAGAAG GTGCTAGACA TACGAGAGAC AGAAGATTGC
    CATAATGCTT TTGCATTGCT TGTGAGGCCA CCAACAGAGC AAGCAAATGT GCTGCTCAGT
    TTCCAGATGA CATCAGAGGA ACTTCCAAAA GAAAACTGGC TAAAGATGCT ATGTCGACAT
    GTAGCCAACA CCATTTGTAA AGCAGATGCT GAGAATCTTA TTTACACTGC TGATCCAGAA
    TCCTTTGAAG TAAATACAAA AGATATGGAC AGTACATTGA GTAGAGCATC TAGAGCAATA
    AAAAAGACTT CAAAAAAGGT TACCAGAGCA TTCTCTTTCT CCAAAACTCC AAAAAGAGCT
    CTCCGAAGGG CTCTTATGGC ATCCCAAAGT TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT
    GAGAAGCATG TAATGAGTCG TCTGGCTAGC ACATCATCAT TAGCAGGCAT CCCGTCTCCC
    TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA AGGAGAAGCC ATACTTTAAG TAGATCTACA
    ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46075
    Clone ID Related Accession (Same CDS sequence) XM_010801363.2
    Accession Version XM_010801363.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTGGATCG TGACCTTTGA AGAAACTGGT GATAGAGACA GTTGTCTGGA ACTGTTGTTT 
    TTCACCAACC ATCATAGAAG AGAACAAACA ATGGCTGATG ATAGTGCATT AGCATCCACT
    ACTGGCAGTA CAAGCTTGGC AGACTCTTCC ATTTTTGATT CTAAAGTTGC CGAAACTTCT
    AAGGAAAACT TATTTATTAG ATCTACTTCA TGTGTTGAAG AAGAAATGCC TCAGATTGAA
    ACAAGAGTCA TACTGGTTCA AGAATCTGGA AAACAAGAAG AACTTATAAG AGCCTTAAAG
    ACTATTAACA TAATGGAAGT CCCTGTTATA AAGATAAAAG AAAGTTGTCC TGGAAAATCG
    GATGAAAAAT TAATAAAAAG TATTGTTAAT ATGGAAATTA AACTGCCGTT TGTAAAGATG
    GAATCAGTTG AAGAATTTAA AAGTTTGGAT TCTCCAGAAT TTGAAAATGT ATTCATAGTC
    ATGGACTTCC AGGATTCTGT CTTTAATGAG TTATACAAGG CTGATTGTAG AGTTATTGGA
    CCACCAGTTG TATTAAACTG TGCAGAAAAA GGAGAGCCTT TGCCATTTTC ATGTCGCCCA
    CTCTATTGTA CCAGCATGAT GAATCTAGTA CTATGTTTTA CTGGATTCAG GAAGAAAGAA
    GAACTAGCCA GATTGGTGAC ATTGGTTCAT CACATGGGTG GAGTTATTCG AAAAGACTTT
    AATTCAAAAG TTACACATTT GGTGGCAAAT TGTACACAAG GAGAAAAATT CAGGGTAGCT
    GTGAGCCTGG GTACTCCAAT TATGAAACCA GAATGGATTC ATAAAGCTTG GGAAAGGCGG
    AATGAACAGG ACTTCTGTGC ATCAGTTGAT GACTTTAGAA ATGAATTTAA AGTTCCTCCA
    TTTCAAGATT GCATTTTAAG TTTCCTGGGT TTTTCGGATG AAGAGAAAAC CAATATGGAA
    GAAATGACTA AAATGCAAGG AGGAAACTGT TTACCAGTTG GAGATGAAAG ATGCACTCAC
    CTTATAGTTG AAGAGAATAT AGTAAAAGAA CTTCCATTTG AACCTTCAAA GAAACTTTAT
    GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC ATTCAAATGG ATGCCCGAGC TGGAGAAACT
    ATGTATTTAT ATGAAAAGGC CAATACACCT GAGCTCAAGA AATCAGTGTC ACTGCTTTCT
    CTAAATACCC CAAACAGCAA TCGCAAAAGA CGTCGTTTAA AAGAGACACT TGCTCAGCTT
    TCAAGAGAGG CAGACTTGTC ACCTTTCCCT CCCCAAACAC CAAAATCTTG TACTAAGTCC
    TCTAAGAATT CTACTCCAGT TCCTTCAAAG CAGTCAGCCA GGTGGCAAGT TGCAAAAGAG
    CTCTATCAGA CTGAAAGTAA TTATGTAAAT ATATTGGCCA CAATTATTCA GTTATTTCAA
    GTACCATTGG AAGAGGAAGG ACAACGAGGT GGGCCTATCC TTGCACCGGA AGAAATTAAG
    ACGATTTTTG GTAGTATTCC AGATATCTTT GATGTGCACA CTAAGATAAA GGATGATCTT
    GAAGACCTTA TTGTTAATTG GGATGAGAGC AAAAGCATTG GTGATATCTT TCTTAAATAT
    TCCAAAGATT TGGTAAAAAT CTATCCTCCC TTTGTGAACT TCTTTGAAAT GAGCAAGGAA
    ACTATTATTA AATGTGAAAA ACAGAAACCA AGATTTCATG CTTTTCTGAA GATAAACCAA
    GCTAAGCCAG AATGTGGACG GCAAAGCCTT GTTGAACTTC TCATCCGACC AGTGCAGAGA
    TTACCCAGCA TTGCATTACT TTTAAATGAT CTTAAGAAGC ACACATTTGA AGAAAATCCT
    GACAAAAGCA CTCTAGAAAA AGCTATTGAA TCACTAAAGG AAGTAATGAC CCATATTAAT
    GAGGATAAGA GAAAAACAGA AGCTCAAAGG CAAATTTTCG ATGTTGTTTA TGAGGTAGAT
    GGATGTCCAG CTAATCTTTT ATCTTCTCAC CGAAGTTTAG TTCAGCGAGT TGAAACAATT
    TCTCTAGGTG AGCACCCCTG TGACAGAGGA GAACAAGTAA CTCTCTTCCT CTTCAATGAC
    TGCCTGGAGA TAGCAAGAAA ACGACACAAG GTTATTGGTA CTTTTAGGAG TCCTCATGGC
    CAGACCCGAC CCCCAGCTTC TCTTAAGCAT ATTCATCTAA TGCCTCTTTC TCAGATTAAG
    AAGGTGCTAG ACATACGAGA GACAGAAGAT TGCCATAATG CTTTTGCATT GCTTGTGAGG
    CCACCAACAG AGCAAGCAAA TGTGCTGCTC AGTTTCCAGA TGACATCAGA GGAACTTCCA
    AAAGAAAACT GGCTAAAGAT GCTATGTCGA CATGTAGCCA ACACCATTTG TAAAGCAGAT
    GCTGAGAATC TTATTTACAC TGCTGATCCA GAATCCTTTG AAGTAAATAC AAAAGATATG
    GACAGTACAT TGAGTAGAGC ATCTAGAGCA ATAAAAAAGA CTTCAAAAAA GGTTACCAGA
    GCATTCTCTT TCTCCAAAAC TCCAAAAAGA GCTCTCCGAA GGGCTCTTAT GGCATCCCAA
    AGTTCAGCAG AGGGAAGAAG TCCTTCTAGC AATGAGAAGC ATGTAATGAG TCGTCTGGCT
    AGCACATCAT CATTAGCAGG CATCCCGTCT CCCTCCCTTG TCAGTCTTCC TGCCTTCTTT
    GAAAGGAGAA GCCATACTTT AAGTAGATCT ACAACTCATT TGATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799665.1
    CDS176..2923
    Translation

    Target ORF information:

    RefSeq Version XM_010801363.2
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801363.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTGGATCG TGACCTTTGA AGAAACTGGT GATAGAGACA GTTGTCTGGA ACTGTTGTTT 
    TTCACCAACC ATCATAGAAG AGAACAAACA ATGGCTGATG ATAGTGCATT AGCATCCACT
    ACTGGCAGTA CAAGCTTGGC AGACTCTTCC ATTTTTGATT CTAAAGTTGC CGAAACTTCT
    AAGGAAAACT TATTTATTAG ATCTACTTCA TGTGTTGAAG AAGAAATGCC TCAGATTGAA
    ACAAGAGTCA TACTGGTTCA AGAATCTGGA AAACAAGAAG AACTTATAAG AGCCTTAAAG
    ACTATTAACA TAATGGAAGT CCCTGTTATA AAGATAAAAG AAAGTTGTCC TGGAAAATCG
    GATGAAAAAT TAATAAAAAG TATTGTTAAT ATGGAAATTA AACTGCCGTT TGTAAAGATG
    GAATCAGTTG AAGAATTTAA AAGTTTGGAT TCTCCAGAAT TTGAAAATGT ATTCATAGTC
    ATGGACTTCC AGGATTCTGT CTTTAATGAG TTATACAAGG CTGATTGTAG AGTTATTGGA
    CCACCAGTTG TATTAAACTG TGCAGAAAAA GGAGAGCCTT TGCCATTTTC ATGTCGCCCA
    CTCTATTGTA CCAGCATGAT GAATCTAGTA CTATGTTTTA CTGGATTCAG GAAGAAAGAA
    GAACTAGCCA GATTGGTGAC ATTGGTTCAT CACATGGGTG GAGTTATTCG AAAAGACTTT
    AATTCAAAAG TTACACATTT GGTGGCAAAT TGTACACAAG GAGAAAAATT CAGGGTAGCT
    GTGAGCCTGG GTACTCCAAT TATGAAACCA GAATGGATTC ATAAAGCTTG GGAAAGGCGG
    AATGAACAGG ACTTCTGTGC ATCAGTTGAT GACTTTAGAA ATGAATTTAA AGTTCCTCCA
    TTTCAAGATT GCATTTTAAG TTTCCTGGGT TTTTCGGATG AAGAGAAAAC CAATATGGAA
    GAAATGACTA AAATGCAAGG AGGAAACTGT TTACCAGTTG GAGATGAAAG ATGCACTCAC
    CTTATAGTTG AAGAGAATAT AGTAAAAGAA CTTCCATTTG AACCTTCAAA GAAACTTTAT
    GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC ATTCAAATGG ATGCCCGAGC TGGAGAAACT
    ATGTATTTAT ATGAAAAGGC CAATACACCT GAGCTCAAGA AATCAGTGTC ACTGCTTTCT
    CTAAATACCC CAAACAGCAA TCGCAAAAGA CGTCGTTTAA AAGAGACACT TGCTCAGCTT
    TCAAGAGAGG CAGACTTGTC ACCTTTCCCT CCCCAAACAC CAAAATCTTG TACTAAGTCC
    TCTAAGAATT CTACTCCAGT TCCTTCAAAG CAGTCAGCCA GGTGGCAAGT TGCAAAAGAG
    CTCTATCAGA CTGAAAGTAA TTATGTAAAT ATATTGGCCA CAATTATTCA GTTATTTCAA
    GTACCATTGG AAGAGGAAGG ACAACGAGGT GGGCCTATCC TTGCACCGGA AGAAATTAAG
    ACGATTTTTG GTAGTATTCC AGATATCTTT GATGTGCACA CTAAGATAAA GGATGATCTT
    GAAGACCTTA TTGTTAATTG GGATGAGAGC AAAAGCATTG GTGATATCTT TCTTAAATAT
    TCCAAAGATT TGGTAAAAAT CTATCCTCCC TTTGTGAACT TCTTTGAAAT GAGCAAGGAA
    ACTATTATTA AATGTGAAAA ACAGAAACCA AGATTTCATG CTTTTCTGAA GATAAACCAA
    GCTAAGCCAG AATGTGGACG GCAAAGCCTT GTTGAACTTC TCATCCGACC AGTGCAGAGA
    TTACCCAGCA TTGCATTACT TTTAAATGAT CTTAAGAAGC ACACATTTGA AGAAAATCCT
    GACAAAAGCA CTCTAGAAAA AGCTATTGAA TCACTAAAGG AAGTAATGAC CCATATTAAT
    GAGGATAAGA GAAAAACAGA AGCTCAAAGG CAAATTTTCG ATGTTGTTTA TGAGGTAGAT
    GGATGTCCAG CTAATCTTTT ATCTTCTCAC CGAAGTTTAG TTCAGCGAGT TGAAACAATT
    TCTCTAGGTG AGCACCCCTG TGACAGAGGA GAACAAGTAA CTCTCTTCCT CTTCAATGAC
    TGCCTGGAGA TAGCAAGAAA ACGACACAAG GTTATTGGTA CTTTTAGGAG TCCTCATGGC
    CAGACCCGAC CCCCAGCTTC TCTTAAGCAT ATTCATCTAA TGCCTCTTTC TCAGATTAAG
    AAGGTGCTAG ACATACGAGA GACAGAAGAT TGCCATAATG CTTTTGCATT GCTTGTGAGG
    CCACCAACAG AGCAAGCAAA TGTGCTGCTC AGTTTCCAGA TGACATCAGA GGAACTTCCA
    AAAGAAAACT GGCTAAAGAT GCTATGTCGA CATGTAGCCA ACACCATTTG TAAAGCAGAT
    GCTGAGAATC TTATTTACAC TGCTGATCCA GAATCCTTTG AAGTAAATAC AAAAGATATG
    GACAGTACAT TGAGTAGAGC ATCTAGAGCA ATAAAAAAGA CTTCAAAAAA GGTTACCAGA
    GCATTCTCTT TCTCCAAAAC TCCAAAAAGA GCTCTCCGAA GGGCTCTTAT GGCATCCCAA
    AGTTCAGCAG AGGGAAGAAG TCCTTCTAGC AATGAGAAGC ATGTAATGAG TCGTCTGGCT
    AGCACATCAT CATTAGCAGG CATCCCGTCT CCCTCCCTTG TCAGTCTTCC TGCCTTCTTT
    GAAAGGAGAA GCCATACTTT AAGTAGATCT ACAACTCATT TGATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46076
    Clone ID Related Accession (Same CDS sequence) XM_010801366.2 , XM_010801365.2 , XM_010801364.3 , XM_010801364.2 , XM_010801365.3
    Accession Version XM_010801366.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801366.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799668.1
    CDS152..2896
    Translation

    Target ORF information:

    RefSeq Version XM_010801366.2
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801366.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46076
    Clone ID Related Accession (Same CDS sequence) XM_010801366.2 , XM_010801365.2 , XM_010801364.3 , XM_010801364.2 , XM_010801365.3
    Accession Version XM_010801365.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799667.1
    CDS124..2868
    Translation

    Target ORF information:

    RefSeq Version XM_010801365.2
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801365.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46076
    Clone ID Related Accession (Same CDS sequence) XM_010801366.2 , XM_010801365.2 , XM_010801364.3 , XM_010801364.2 , XM_010801365.3
    Accession Version XM_010801364.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801364.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799666.1
    CDS159..2903
    Translation

    Target ORF information:

    RefSeq Version XM_010801364.3
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801364.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46076
    Clone ID Related Accession (Same CDS sequence) XM_010801366.2 , XM_010801365.2 , XM_010801364.3 , XM_010801364.2 , XM_010801365.3
    Accession Version XM_010801364.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799666.1
    CDS150..2894
    Translation

    Target ORF information:

    RefSeq Version XM_010801364.2
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801364.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46076
    Clone ID Related Accession (Same CDS sequence) XM_010801366.2 , XM_010801365.2 , XM_010801364.3 , XM_010801364.2 , XM_010801365.3
    Accession Version XM_010801365.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801365.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799667.1
    CDS163..2907
    Translation

    Target ORF information:

    RefSeq Version XM_010801365.3
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801365.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCGTAAGC GCCCTTCAGC TGAACATTCC CTTTCTATAG GGTCACTCCT AGATATCTCC
    AACACACCAG AATCTAGCAT TAACTATGGA GAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46077
    Clone ID Related Accession (Same CDS sequence) XM_015472466.1
    Accession Version XM_015472466.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103817.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTGGATCG TGACCTTTGA AGAAACTGGT GATAGAGACA GTTGTCTGGA ACTGTTGTTT 
    TTCACCAACC ATCATAGAAG AGAACAAACA ATGGCTGATG ATAGTGCATT AGCATCCACT
    ACTGGCAGTA CAAGCTTGGC AGACTCTTCC ATTTTTGATT CTAAAGTTGC CGAAACTTCT
    AAGGAAAACT TATTTATTAG ATCTACTTCA TGTGTTGAAG AAGAAATGCC TCAGATTGAA
    ACAAGAGTCA TACTGGTTCA AGAATCTGGA AAACAAGAAG AACTTATAAG AGCCTTAAAG
    GAAATTAAAC TGCCGTTTGT AAAGATGGAA TCAGTTGAAG AATTTAAAAG TTTGGATTCT
    CCAGAATTTG AAAATGTATT CATAGTCATG GACTTCCAGG ATTCTGTCTT TAATGAGTTA
    TACAAGGCTG ATTGTAGAGT TATTGGACCA CCAGTTGTAT TAAACTGTGC AGAAAAAGGA
    GAGCCTTTGC CATTTTCATG TCGCCCACTC TATTGTACCA GCATGATGAA TCTAGTACTA
    TGTTTTACTG GATTCAGGAA GAAAGAAGAA CTAGCCAGAT TGGTGACATT GGTTCATCAC
    ATGGGTGGAG TTATTCGAAA AGACTTTAAT TCAAAAGTTA CACATTTGGT GGCAAATTGT
    ACACAAGGAG AAAAATTCAG GGTAGCTGTG AGCCTGGGTA CTCCAATTAT GAAACCAGAA
    TGGATTCATA AAGCTTGGGA AAGGCGGAAT GAACAGGACT TCTGTGCATC AGTTGATGAC
    TTTAGAAATG AATTTAAAGT TCCTCCATTT CAAGATTGCA TTTTAAGTTT CCTGGGTTTT
    TCGGATGAAG AGAAAACCAA TATGGAAGAA ATGACTAAAA TGCAAGGAGG AAACTGTTTA
    CCAGTTGGAG ATGAAAGATG CACTCACCTT ATAGTTGAAG AGAATATAGT AAAAGAACTT
    CCATTTGAAC CTTCAAAGAA ACTTTATGTT GTGAAACAAG AGTGGTTCTG GGGAAGCATT
    CAAATGGATG CCCGAGCTGG AGAAACTATG TATTTATATG AAAAGGCCAA TACACCTGAG
    CTCAAGAAAT CAGTGTCACT GCTTTCTCTA AATACCCCAA ACAGCAATCG CAAAAGACGT
    CGTTTAAAAG AGACACTTGC TCAGCTTTCA AGAGAGGCAG ACTTGTCACC TTTCCCTCCC
    CGTAAGCGCC CTTCAGCTGA ACATTCCCTT TCTATAGGGT CACTCCTAGA TATCTCCAAC
    ACACCAGAAT CTAGCATTAA CTATGGAGAA ACACCAAAAT CTTGTACTAA GTCCTCTAAG
    AATTCTACTC CAGTTCCTTC AAAGCAGTCA GCCAGGTGGC AAGTTGCAAA AGAGCTCTAT
    CAGACTGAAA GTAATTATGT AAATATATTG GCCACAATTA TTCAGTTATT TCAAGTACCA
    TTGGAAGAGG AAGGACAACG AGGTGGGCCT ATCCTTGCAC CGGAAGAAAT TAAGACGATT
    TTTGGTAGTA TTCCAGATAT CTTTGATGTG CACACTAAGA TAAAGGATGA TCTTGAAGAC
    CTTATTGTTA ATTGGGATGA GAGCAAAAGC ATTGGTGATA TCTTTCTTAA ATATTCCAAA
    GATTTGGTAA AAATCTATCC TCCCTTTGTG AACTTCTTTG AAATGAGCAA GGAAACTATT
    ATTAAATGTG AAAAACAGAA ACCAAGATTT CATGCTTTTC TGAAGATAAA CCAAGCTAAG
    CCAGAATGTG GACGGCAAAG CCTTGTTGAA CTTCTCATCC GACCAGTGCA GAGATTACCC
    AGCATTGCAT TACTTTTAAA TGATCTTAAG AAGCACACAT TTGAAGAAAA TCCTGACAAA
    AGCACTCTAG AAAAAGCTAT TGAATCACTA AAGGAAGTAA TGACCCATAT TAATGAGGAT
    AAGAGAAAAA CAGAAGCTCA AAGGCAAATT TTCGATGTTG TTTATGAGGT AGATGGATGT
    CCAGCTAATC TTTTATCTTC TCACCGAAGT TTAGTTCAGC GAGTTGAAAC AATTTCTCTA
    GGTGAGCACC CCTGTGACAG AGGAGAACAA GTAACTCTCT TCCTCTTCAA TGACTGCCTG
    GAGATAGCAA GAAAACGACA CAAGGTTATT GGTACTTTTA GGAGTCCTCA TGGCCAGACC
    CGACCCCCAG CTTCTCTTAA GCATATTCAT CTAATGCCTC TTTCTCAGAT TAAGAAGGTG
    CTAGACATAC GAGAGACAGA AGATTGCCAT AATGCTTTTG CATTGCTTGT GAGGCCACCA
    ACAGAGCAAG CAAATGTGCT GCTCAGTTTC CAGATGACAT CAGAGGAACT TCCAAAAGAA
    AACTGGCTAA AGATGCTATG TCGACATGTA GCCAACACCA TTTGTAAAGC AGATGCTGAG
    AATCTTATTT ACACTGCTGA TCCAGAATCC TTTGAAGTAA ATACAAAAGA TATGGACAGT
    ACATTGAGTA GAGCATCTAG AGCAATAAAA AAGACTTCAA AAAAGGTTAC CAGAGCATTC
    TCTTTCTCCA AAACTCCAAA AAGAGCTCTC CGAAGGGCTC TTATGGCATC CCAAAGTTCA
    GCAGAGGGAA GAAGTCCTTC TAGCAATGAG AAGCATGTAA TGAGTCGTCT GGCTAGCACA
    TCATCATTAG CAGGCATCCC GTCTCCCTCC CTTGTCAGTC TTCCTGCCTT CTTTGAAAGG
    AGAAGCCATA CTTTAAGTAG ATCTACAACT CATTTGATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327952.1
    CDS176..2917
    Translation

    Target ORF information:

    RefSeq Version XM_015472466.1
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472466.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTGGATCG TGACCTTTGA AGAAACTGGT GATAGAGACA GTTGTCTGGA ACTGTTGTTT 
    TTCACCAACC ATCATAGAAG AGAACAAACA ATGGCTGATG ATAGTGCATT AGCATCCACT
    ACTGGCAGTA CAAGCTTGGC AGACTCTTCC ATTTTTGATT CTAAAGTTGC CGAAACTTCT
    AAGGAAAACT TATTTATTAG ATCTACTTCA TGTGTTGAAG AAGAAATGCC TCAGATTGAA
    ACAAGAGTCA TACTGGTTCA AGAATCTGGA AAACAAGAAG AACTTATAAG AGCCTTAAAG
    GAAATTAAAC TGCCGTTTGT AAAGATGGAA TCAGTTGAAG AATTTAAAAG TTTGGATTCT
    CCAGAATTTG AAAATGTATT CATAGTCATG GACTTCCAGG ATTCTGTCTT TAATGAGTTA
    TACAAGGCTG ATTGTAGAGT TATTGGACCA CCAGTTGTAT TAAACTGTGC AGAAAAAGGA
    GAGCCTTTGC CATTTTCATG TCGCCCACTC TATTGTACCA GCATGATGAA TCTAGTACTA
    TGTTTTACTG GATTCAGGAA GAAAGAAGAA CTAGCCAGAT TGGTGACATT GGTTCATCAC
    ATGGGTGGAG TTATTCGAAA AGACTTTAAT TCAAAAGTTA CACATTTGGT GGCAAATTGT
    ACACAAGGAG AAAAATTCAG GGTAGCTGTG AGCCTGGGTA CTCCAATTAT GAAACCAGAA
    TGGATTCATA AAGCTTGGGA AAGGCGGAAT GAACAGGACT TCTGTGCATC AGTTGATGAC
    TTTAGAAATG AATTTAAAGT TCCTCCATTT CAAGATTGCA TTTTAAGTTT CCTGGGTTTT
    TCGGATGAAG AGAAAACCAA TATGGAAGAA ATGACTAAAA TGCAAGGAGG AAACTGTTTA
    CCAGTTGGAG ATGAAAGATG CACTCACCTT ATAGTTGAAG AGAATATAGT AAAAGAACTT
    CCATTTGAAC CTTCAAAGAA ACTTTATGTT GTGAAACAAG AGTGGTTCTG GGGAAGCATT
    CAAATGGATG CCCGAGCTGG AGAAACTATG TATTTATATG AAAAGGCCAA TACACCTGAG
    CTCAAGAAAT CAGTGTCACT GCTTTCTCTA AATACCCCAA ACAGCAATCG CAAAAGACGT
    CGTTTAAAAG AGACACTTGC TCAGCTTTCA AGAGAGGCAG ACTTGTCACC TTTCCCTCCC
    CGTAAGCGCC CTTCAGCTGA ACATTCCCTT TCTATAGGGT CACTCCTAGA TATCTCCAAC
    ACACCAGAAT CTAGCATTAA CTATGGAGAA ACACCAAAAT CTTGTACTAA GTCCTCTAAG
    AATTCTACTC CAGTTCCTTC AAAGCAGTCA GCCAGGTGGC AAGTTGCAAA AGAGCTCTAT
    CAGACTGAAA GTAATTATGT AAATATATTG GCCACAATTA TTCAGTTATT TCAAGTACCA
    TTGGAAGAGG AAGGACAACG AGGTGGGCCT ATCCTTGCAC CGGAAGAAAT TAAGACGATT
    TTTGGTAGTA TTCCAGATAT CTTTGATGTG CACACTAAGA TAAAGGATGA TCTTGAAGAC
    CTTATTGTTA ATTGGGATGA GAGCAAAAGC ATTGGTGATA TCTTTCTTAA ATATTCCAAA
    GATTTGGTAA AAATCTATCC TCCCTTTGTG AACTTCTTTG AAATGAGCAA GGAAACTATT
    ATTAAATGTG AAAAACAGAA ACCAAGATTT CATGCTTTTC TGAAGATAAA CCAAGCTAAG
    CCAGAATGTG GACGGCAAAG CCTTGTTGAA CTTCTCATCC GACCAGTGCA GAGATTACCC
    AGCATTGCAT TACTTTTAAA TGATCTTAAG AAGCACACAT TTGAAGAAAA TCCTGACAAA
    AGCACTCTAG AAAAAGCTAT TGAATCACTA AAGGAAGTAA TGACCCATAT TAATGAGGAT
    AAGAGAAAAA CAGAAGCTCA AAGGCAAATT TTCGATGTTG TTTATGAGGT AGATGGATGT
    CCAGCTAATC TTTTATCTTC TCACCGAAGT TTAGTTCAGC GAGTTGAAAC AATTTCTCTA
    GGTGAGCACC CCTGTGACAG AGGAGAACAA GTAACTCTCT TCCTCTTCAA TGACTGCCTG
    GAGATAGCAA GAAAACGACA CAAGGTTATT GGTACTTTTA GGAGTCCTCA TGGCCAGACC
    CGACCCCCAG CTTCTCTTAA GCATATTCAT CTAATGCCTC TTTCTCAGAT TAAGAAGGTG
    CTAGACATAC GAGAGACAGA AGATTGCCAT AATGCTTTTG CATTGCTTGT GAGGCCACCA
    ACAGAGCAAG CAAATGTGCT GCTCAGTTTC CAGATGACAT CAGAGGAACT TCCAAAAGAA
    AACTGGCTAA AGATGCTATG TCGACATGTA GCCAACACCA TTTGTAAAGC AGATGCTGAG
    AATCTTATTT ACACTGCTGA TCCAGAATCC TTTGAAGTAA ATACAAAAGA TATGGACAGT
    ACATTGAGTA GAGCATCTAG AGCAATAAAA AAGACTTCAA AAAAGGTTAC CAGAGCATTC
    TCTTTCTCCA AAACTCCAAA AAGAGCTCTC CGAAGGGCTC TTATGGCATC CCAAAGTTCA
    GCAGAGGGAA GAAGTCCTTC TAGCAATGAG AAGCATGTAA TGAGTCGTCT GGCTAGCACA
    TCATCATTAG CAGGCATCCC GTCTCCCTCC CTTGTCAGTC TTCCTGCCTT CTTTGAAAGG
    AGAAGCCATA CTTTAAGTAG ATCTACAACT CATTTGATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218636
    Clone ID Related Accession (Same CDS sequence) XM_024996990.1
    Accession Version XM_024996990.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2658bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCAAACAC CAAAATCTTG TACTAAGTCC TCTAAGAATT CTACTCCAGT TCCTTCAAAG
    CAGTCAGCCA GGTGGCAAGT TGCAAAAGAG CTCTATCAGA CTGAAAGTAA TTATGTAAAT
    ATATTGGCCA CAATTATTCA GTTATTTCAA GTACCATTGG AAGAGGAAGG ACAACGAGGT
    GGGCCTATCC TTGCACCGGA AGAAATTAAG ACGATTTTTG GTAGTATTCC AGATATCTTT
    GATGTGCACA CTAAGATAAA GGATGATCTT GAAGACCTTA TTGTTAATTG GGATGAGAGC
    AAAAGCATTG GTGATATCTT TCTTAAATAT TCCAAAGATT TGGTAAAAAT CTATCCTCCC
    TTTGTGAACT TCTTTGAAAT GAGCAAGGAA ACTATTATTA AATGTGAAAA ACAGAAACCA
    AGATTTCATG CTTTTCTGAA GATAAACCAA GCTAAGCCAG AATGTGGACG GCAAAGCCTT
    GTTGAACTTC TCATCCGACC AGTGCAGAGA TTACCCAGCA TTGCATTACT TTTAAATGAT
    CTTAAGAAGC ACACATTTGA AGAAAATCCT GACAAAAGCA CTCTAGAAAA AGCTATTGAA
    TCACTAAAGG AAGTAATGAC CCATATTAAT GAGGATAAGA GAAAAACAGA AGCTCAAAGG
    CAAATTTTCG ATGTTGTTTA TGAGGTAGAT GGATGTCCAG CTAATCTTTT ATCTTCTCAC
    CGAAGTTTAG TTCAGCGAGT TGAAACAATT TCTCTAGGTG AGCACCCCTG TGACAGAGGA
    GAACAAGTAA CTCTCTTCCT CTTCAATGAC TGCCTGGAGA TAGCAAGAAA ACGACACAAG
    GTTATTGGTA CTTTTAGGAG TCCTCATGGC CAGACCCGAC CCCCAGCTTC TCTTAAGCAT
    ATTCATCTAA TGCCTCTTTC TCAGATTAAG AAGGTGCTAG ACATACGAGA GACAGAAGAT
    TGCCATAATG CTTTTGCATT GCTTGTGAGG CCACCAACAG AGCAAGCAAA TGTGCTGCTC
    AGTTTCCAGA TGACATCAGA GGAACTTCCA AAAGAAAACT GGCTAAAGAT GCTATGTCGA
    CATGTAGCCA ACACCATTTG TAAAGCAGAT GCTGAGAATC TTATTTACAC TGCTGATCCA
    GAATCCTTTG AAGTAAATAC AAAAGATATG GACAGTACAT TGAGTAGAGC ATCTAGAGCA
    ATAAAAAAGA CTTCAAAAAA GGTTACCAGA GCATTCTCTT TCTCCAAAAC TCCAAAAAGA
    GCTCTCCGAA GGGCTCTTAT GGCATCCCAA AGTTCAGCAG AGGGAAGAAG TCCTTCTAGC
    AATGAGAAGC ATGTAATGAG TCGTCTGGCT AGCACATCAT CATTAGCAGG CATCCCGTCT
    CCCTCCCTTG TCAGTCTTCC TGCCTTCTTT GAAAGGAGAA GCCATACTTT AAGTAGATCT
    ACAACTCATT TGATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852758.1
    CDS160..2817
    Translation

    Target ORF information:

    RefSeq Version XM_024996990.1
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996990.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG ACTATTAACA TAATGGAAGT CCCTGTTATA
    AAGATAAAAG AAAGTTGTCC TGGAAAATCG GATGAAAAAT TAATAAAAAG TATTGTTAAT
    ATGGAAATTA AACTGCCGTT TGTAAAGATG GAATCAGTTG AAGAATTTAA AAGTTTGGAT
    TCTCCAGAAT TTGAAAATGT ATTCATAGTC ATGGACTTCC AGGATTCTGT CTTTAATGAG
    TTATACAAGG CTGATTGTAG AGTTATTGGA CCACCAGTTG TATTAAACTG TGCAGAAAAA
    GGAGAGCCTT TGCCATTTTC ATGTCGCCCA CTCTATTGTA CCAGCATGAT GAATCTAGTA
    CTATGTTTTA CTGGATTCAG GAAGAAAGAA GAACTAGCCA GATTGGTGAC ATTGGTTCAT
    CACATGGGTG GAGTTATTCG AAAAGACTTT AATTCAAAAG TTACACATTT GGTGGCAAAT
    TGTACACAAG GAGAAAAATT CAGGGTAGCT GTGAGCCTGG GTACTCCAAT TATGAAACCA
    GAATGGATTC ATAAAGCTTG GGAAAGGCGG AATGAACAGG ACTTCTGTGC ATCAGTTGAT
    GACTTTAGAA ATGAATTTAA AGTTCCTCCA TTTCAAGATT GCATTTTAAG TTTCCTGGGT
    TTTTCGGATG AAGAGAAAAC CAATATGGAA GAAATGACTA AAATGCAAGG AGGAAACTGT
    TTACCAGTTG GAGATGAAAG ATGCACTCAC CTTATAGTTG AAGAGAATAT AGTAAAAGAA
    CTTCCATTTG AACCTTCAAA GAAACTTTAT GTTGTGAAAC AAGAGTGGTT CTGGGGAAGC
    ATTCAAATGG ATGCCCGAGC TGGAGAAACT ATGTATTTAT ATGAAAAGGC CAATACACCT
    GAGCTCAAGA AATCAGTGTC ACTGCTTTCT CTAAATACCC CAAACAGCAA TCGCAAAAGA
    CGTCGTTTAA AAGAGACACT TGCTCAGCTT TCAAGAGAGG CAGACTTGTC ACCTTTCCCT
    CCCCAAACAC CAAAATCTTG TACTAAGTCC TCTAAGAATT CTACTCCAGT TCCTTCAAAG
    CAGTCAGCCA GGTGGCAAGT TGCAAAAGAG CTCTATCAGA CTGAAAGTAA TTATGTAAAT
    ATATTGGCCA CAATTATTCA GTTATTTCAA GTACCATTGG AAGAGGAAGG ACAACGAGGT
    GGGCCTATCC TTGCACCGGA AGAAATTAAG ACGATTTTTG GTAGTATTCC AGATATCTTT
    GATGTGCACA CTAAGATAAA GGATGATCTT GAAGACCTTA TTGTTAATTG GGATGAGAGC
    AAAAGCATTG GTGATATCTT TCTTAAATAT TCCAAAGATT TGGTAAAAAT CTATCCTCCC
    TTTGTGAACT TCTTTGAAAT GAGCAAGGAA ACTATTATTA AATGTGAAAA ACAGAAACCA
    AGATTTCATG CTTTTCTGAA GATAAACCAA GCTAAGCCAG AATGTGGACG GCAAAGCCTT
    GTTGAACTTC TCATCCGACC AGTGCAGAGA TTACCCAGCA TTGCATTACT TTTAAATGAT
    CTTAAGAAGC ACACATTTGA AGAAAATCCT GACAAAAGCA CTCTAGAAAA AGCTATTGAA
    TCACTAAAGG AAGTAATGAC CCATATTAAT GAGGATAAGA GAAAAACAGA AGCTCAAAGG
    CAAATTTTCG ATGTTGTTTA TGAGGTAGAT GGATGTCCAG CTAATCTTTT ATCTTCTCAC
    CGAAGTTTAG TTCAGCGAGT TGAAACAATT TCTCTAGGTG AGCACCCCTG TGACAGAGGA
    GAACAAGTAA CTCTCTTCCT CTTCAATGAC TGCCTGGAGA TAGCAAGAAA ACGACACAAG
    GTTATTGGTA CTTTTAGGAG TCCTCATGGC CAGACCCGAC CCCCAGCTTC TCTTAAGCAT
    ATTCATCTAA TGCCTCTTTC TCAGATTAAG AAGGTGCTAG ACATACGAGA GACAGAAGAT
    TGCCATAATG CTTTTGCATT GCTTGTGAGG CCACCAACAG AGCAAGCAAA TGTGCTGCTC
    AGTTTCCAGA TGACATCAGA GGAACTTCCA AAAGAAAACT GGCTAAAGAT GCTATGTCGA
    CATGTAGCCA ACACCATTTG TAAAGCAGAT GCTGAGAATC TTATTTACAC TGCTGATCCA
    GAATCCTTTG AAGTAAATAC AAAAGATATG GACAGTACAT TGAGTAGAGC ATCTAGAGCA
    ATAAAAAAGA CTTCAAAAAA GGTTACCAGA GCATTCTCTT TCTCCAAAAC TCCAAAAAGA
    GCTCTCCGAA GGGCTCTTAT GGCATCCCAA AGTTCAGCAG AGGGAAGAAG TCCTTCTAGC
    AATGAGAAGC ATGTAATGAG TCGTCTGGCT AGCACATCAT CATTAGCAGG CATCCCGTCT
    CCCTCCCTTG TCAGTCTTCC TGCCTTCTTT GAAAGGAGAA GCCATACTTT AAGTAGATCT
    ACAACTCATT TGATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218637
    Clone ID Related Accession (Same CDS sequence) XM_024997128.1
    Accession Version XM_024997128.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ECT2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG GAAATTAAAC TGCCGTTTGT AAAGATGGAA
    TCAGTTGAAG AATTTAAAAG TTTGGATTCT CCAGAATTTG AAAATGTATT CATAGTCATG
    GACTTCCAGG ATTCTGTCTT TAATGAGTTA TACAAGGCTG ATTGTAGAGT TATTGGACCA
    CCAGTTGTAT TAAACTGTGC AGAAAAAGGA GAGCCTTTGC CATTTTCATG TCGCCCACTC
    TATTGTACCA GCATGATGAA TCTAGTACTA TGTTTTACTG GATTCAGGAA GAAAGAAGAA
    CTAGCCAGAT TGGTGACATT GGTTCATCAC ATGGGTGGAG TTATTCGAAA AGACTTTAAT
    TCAAAAGTTA CACATTTGGT GGCAAATTGT ACACAAGGAG AAAAATTCAG GGTAGCTGTG
    AGCCTGGGTA CTCCAATTAT GAAACCAGAA TGGATTCATA AAGCTTGGGA AAGGCGGAAT
    GAACAGGACT TCTGTGCATC AGTTGATGAC TTTAGAAATG AATTTAAAGT TCCTCCATTT
    CAAGATTGCA TTTTAAGTTT CCTGGGTTTT TCGGATGAAG AGAAAACCAA TATGGAAGAA
    ATGACTAAAA TGCAAGGAGG AAACTGTTTA CCAGTTGGAG ATGAAAGATG CACTCACCTT
    ATAGTTGAAG AGAATATAGT AAAAGAACTT CCATTTGAAC CTTCAAAGAA ACTTTATGTT
    GTGAAACAAG AGTGGTTCTG GGGAAGCATT CAAATGGATG CCCGAGCTGG AGAAACTATG
    TATTTATATG AAAAGGCCAA TACACCTGAG CTCAAGAAAT CAGTGTCACT GCTTTCTCTA
    AATACCCCAA ACAGCAATCG CAAAAGACGT CGTTTAAAAG AGACACTTGC TCAGCTTTCA
    AGAGAGGCAG ACTTGTCACC TTTCCCTCCC CAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852896.1
    CDS160..2724
    Translation

    Target ORF information:

    RefSeq Version XM_024997128.1
    Organism Bos taurus(cattle)
    Definition Bos taurus epithelial cell transforming 2 (ECT2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997128.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCTGATG ATAGTGCATT AGCATCCACT ACTGGCAGTA CAAGCTTGGC AGACTCTTCC 
    ATTTTTGATT CTAAAGTTGC CGAAACTTCT AAGGAAAACT TATTTATTAG ATCTACTTCA
    TGTGTTGAAG AAGAAATGCC TCAGATTGAA ACAAGAGTCA TACTGGTTCA AGAATCTGGA
    AAACAAGAAG AACTTATAAG AGCCTTAAAG GAAATTAAAC TGCCGTTTGT AAAGATGGAA
    TCAGTTGAAG AATTTAAAAG TTTGGATTCT CCAGAATTTG AAAATGTATT CATAGTCATG
    GACTTCCAGG ATTCTGTCTT TAATGAGTTA TACAAGGCTG ATTGTAGAGT TATTGGACCA
    CCAGTTGTAT TAAACTGTGC AGAAAAAGGA GAGCCTTTGC CATTTTCATG TCGCCCACTC
    TATTGTACCA GCATGATGAA TCTAGTACTA TGTTTTACTG GATTCAGGAA GAAAGAAGAA
    CTAGCCAGAT TGGTGACATT GGTTCATCAC ATGGGTGGAG TTATTCGAAA AGACTTTAAT
    TCAAAAGTTA CACATTTGGT GGCAAATTGT ACACAAGGAG AAAAATTCAG GGTAGCTGTG
    AGCCTGGGTA CTCCAATTAT GAAACCAGAA TGGATTCATA AAGCTTGGGA AAGGCGGAAT
    GAACAGGACT TCTGTGCATC AGTTGATGAC TTTAGAAATG AATTTAAAGT TCCTCCATTT
    CAAGATTGCA TTTTAAGTTT CCTGGGTTTT TCGGATGAAG AGAAAACCAA TATGGAAGAA
    ATGACTAAAA TGCAAGGAGG AAACTGTTTA CCAGTTGGAG ATGAAAGATG CACTCACCTT
    ATAGTTGAAG AGAATATAGT AAAAGAACTT CCATTTGAAC CTTCAAAGAA ACTTTATGTT
    GTGAAACAAG AGTGGTTCTG GGGAAGCATT CAAATGGATG CCCGAGCTGG AGAAACTATG
    TATTTATATG AAAAGGCCAA TACACCTGAG CTCAAGAAAT CAGTGTCACT GCTTTCTCTA
    AATACCCCAA ACAGCAATCG CAAAAGACGT CGTTTAAAAG AGACACTTGC TCAGCTTTCA
    AGAGAGGCAG ACTTGTCACC TTTCCCTCCC CAAACACCAA AATCTTGTAC TAAGTCCTCT
    AAGAATTCTA CTCCAGTTCC TTCAAAGCAG TCAGCCAGGT GGCAAGTTGC AAAAGAGCTC
    TATCAGACTG AAAGTAATTA TGTAAATATA TTGGCCACAA TTATTCAGTT ATTTCAAGTA
    CCATTGGAAG AGGAAGGACA ACGAGGTGGG CCTATCCTTG CACCGGAAGA AATTAAGACG
    ATTTTTGGTA GTATTCCAGA TATCTTTGAT GTGCACACTA AGATAAAGGA TGATCTTGAA
    GACCTTATTG TTAATTGGGA TGAGAGCAAA AGCATTGGTG ATATCTTTCT TAAATATTCC
    AAAGATTTGG TAAAAATCTA TCCTCCCTTT GTGAACTTCT TTGAAATGAG CAAGGAAACT
    ATTATTAAAT GTGAAAAACA GAAACCAAGA TTTCATGCTT TTCTGAAGAT AAACCAAGCT
    AAGCCAGAAT GTGGACGGCA AAGCCTTGTT GAACTTCTCA TCCGACCAGT GCAGAGATTA
    CCCAGCATTG CATTACTTTT AAATGATCTT AAGAAGCACA CATTTGAAGA AAATCCTGAC
    AAAAGCACTC TAGAAAAAGC TATTGAATCA CTAAAGGAAG TAATGACCCA TATTAATGAG
    GATAAGAGAA AAACAGAAGC TCAAAGGCAA ATTTTCGATG TTGTTTATGA GGTAGATGGA
    TGTCCAGCTA ATCTTTTATC TTCTCACCGA AGTTTAGTTC AGCGAGTTGA AACAATTTCT
    CTAGGTGAGC ACCCCTGTGA CAGAGGAGAA CAAGTAACTC TCTTCCTCTT CAATGACTGC
    CTGGAGATAG CAAGAAAACG ACACAAGGTT ATTGGTACTT TTAGGAGTCC TCATGGCCAG
    ACCCGACCCC CAGCTTCTCT TAAGCATATT CATCTAATGC CTCTTTCTCA GATTAAGAAG
    GTGCTAGACA TACGAGAGAC AGAAGATTGC CATAATGCTT TTGCATTGCT TGTGAGGCCA
    CCAACAGAGC AAGCAAATGT GCTGCTCAGT TTCCAGATGA CATCAGAGGA ACTTCCAAAA
    GAAAACTGGC TAAAGATGCT ATGTCGACAT GTAGCCAACA CCATTTGTAA AGCAGATGCT
    GAGAATCTTA TTTACACTGC TGATCCAGAA TCCTTTGAAG TAAATACAAA AGATATGGAC
    AGTACATTGA GTAGAGCATC TAGAGCAATA AAAAAGACTT CAAAAAAGGT TACCAGAGCA
    TTCTCTTTCT CCAAAACTCC AAAAAGAGCT CTCCGAAGGG CTCTTATGGC ATCCCAAAGT
    TCAGCAGAGG GAAGAAGTCC TTCTAGCAAT GAGAAGCATG TAATGAGTCG TCTGGCTAGC
    ACATCATCAT TAGCAGGCAT CCCGTCTCCC TCCCTTGTCA GTCTTCCTGC CTTCTTTGAA
    AGGAGAAGCC ATACTTTAAG TAGATCTACA ACTCATTTGA TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.