pbl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pbl
Entrez Gene ID	38879
Full Name	pebble
Synonyms	0083/20,0293/09,0542/03,CG8114,Dmel\CG8114,ECT2,Ect-2,GEF,PBL,Pbl,Pbl/Ect2,anon-WO0172774.22,anon-WO0172774.25,l(3)09645
General protein information	Preferred Names pebble Names CG8114 gene product from transcript CG8114-RA CG8114-PA CG8114-PB CG8114-PC CG8114-PD CG8114-PE CG8114-PF pbl-PA pbl-PB pbl-PC pbl-PD pbl-PE pbl-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66A18-66A19\|3-26 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_168241.3	NP_729306.1	pebble, isoform A
NM_168242.3	NP_729307.1	pebble, isoform C
NM_001104070.2	NP_001097540.1	pebble, isoform E
NM_079241.4	NP_523965.2	pebble, isoform B
NM_168243.3	NP_729308.1	pebble, isoform D
NM_001259732.1	NP_001246661.1	pebble, isoform F

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ECT2	XP_003950278.1
	ECT2	XP_001083830.1
Gallus gallus (chicken)	ECT2	XP_004943527.1
Danio rerio (zebrafish)	ect2	NP_001003883.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005796	XP_556459.3
Homo sapiens (human)	ECT2	NP_001245244.1
Bos taurus (cattle)	ECT2	NP_001091042.1
Rattus norvegicus (Norway rat)	Ect2	NP_001102017.2
Drosophila melanogaster (fruit fly)	pbl	NP_523965.2
Mus musculus (house mouse)	Ect2	NP_031926.2
Canis lupus familiaris (dog)	ECT2	XP_005639979.1
Xenopus tropicalis (tropical clawed frog)	ect2	NP_001106617.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RhoGEF2, Pbl, RhoGEF3, and Tum exhibit discrete localization patterns and are required for cell wound repair.
Title: Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair.

Both Pbl and ECT2 repress Wg/Wnt target gene expression in cultured Drosophila and human cells.
Title: Pebble/ECT2 RhoGEF negatively regulates the Wingless/Wnt signaling pathway.

Reduced cytokinesis may be responsible for the left-right defects in the hindgut of embryos homozygous for pbl.
Title: Reduced cell number in the hindgut epithelium disrupts hindgut left-right asymmetry in a mutant of pebble, encoding a RhoGEF, in Drosophila embryos.

early Drosophila embryogenesis is primed to form furrows at either overlapping astral microtubules or the central spindle; the shift to the latter is driven by a corresponding shift from RhoGEF2 to Pebble in controlling furrow formation.
Title: RhoGEF and positioning of rappaport-like furrows in the early Drosophila embryo.

This study demonistrated that The Control of semaphorin-1a-mediated reverse signaling by opposing pebble and RhoGAPp190 functions in drosophila development.
Title: The Control of semaphorin-1a-mediated reverse signaling by opposing pebble and RhoGAPp190 functions in drosophila.

The following pbl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pbl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa20685	NM_001259732.1 Latest version!	Drosophila melanogaster pebble (pbl), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa17840	NM_168241.3 Latest version!	Drosophila melanogaster pebble (pbl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa17842	NM_001104070.2 Latest version!	Drosophila melanogaster pebble (pbl), transcript variant E, partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa17843	NM_168242.3 Latest version!	Drosophila melanogaster pebble (pbl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa17841	NM_079241.4 Latest version!	Drosophila melanogaster pebble (pbl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa17844	NM_168243.3 Latest version!	Drosophila melanogaster pebble (pbl), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pbl ( NM_001259732.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246661.1 Drosophila melanogaster pebble (pbl), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa20685
Clone ID Related Accession (Same CDS sequence)	NM_001259732.1
Accession Version	NM_001259732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pebble, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTATC TGTTTGGCGA CTATTTGGAT AGCATCACGA ATACACCGAA CACAGATCGT 
CGGGATTCAT TGCCCATTAG CTTTAACAAA AGGAAACGCA AGCGTTTTTC GCAACGCATC 
CAGCTGGAGG GTACTCCGTT GGGTTCTGGT AAACGACGCT CCTCCGTTTC GGATGCCGGT 
CTATTGTCTG TCTCAAATAG TTTATTCGAT TGTACCACCA GTCCGGATAA ACTGGAGTCG 
GACAAGCTGT TGCATGCCGA GCCGGAGGCG AGCGATGCCA CTCCAGCCAA GAAATCGATG 
CGCTTTAATC ACTTTATGGA CTTTTATACC ACAGAGTCCA ACTACGTGGG CATATTGGAC 
ACCATACTGA ATCTTTTTAA AAACAAGTTG GAAGAGTTGG CTGAAACCAA TGATCCGCTG 
CTTAATAAGT CGGAGATCAA GACGATCTTT GGCAACTTCC TGCCTATCCA CGAGGTACAC 
CAAAGCATGC TGGAGCATCT GCGGAAATTG CATGCTAACT GGCGGGAGGA TTGTCTTATT 
GGAGACATTA TCATTCAACA TCGCGACGAG CTGATAAAGG CGTATCCACC ATACGTCAAC 
TTCTTCGAGC AGATGAAGGA GCAACTGCAG TACTGCGATC GGGAATATCC GCGTTTCCAC 
GCCTTTCTGA AGATTAATCA AACGAAACCG GAGTGCGGAC GCCAAGGTCT ACAAGATCTG 
ATGATCCGTC CAGTTCAGCG ATTGCCCAGT ATCAGTCTGC TGCTAAATGA TATATTGAAA 
CATACGACAA GTGGCAATGC GGACCACGGA CGTTTGGAGG AGGCTCTGAA GGCCATCAAG 
CAGGTGACAC TGCACATCAA CGAGGATAAA AGGCGGACCG AATCGCGCAT GGCCATCTTC 
GATATATTCA ACGATATCGA AGGATGTCCG GCGCATTTAG TGAGTTCCAA CCGCAGCTTT 
ATTTTGAAGT GTGAGGTGAA CGAGCTCTCC GATTCGCTGA GCGGTCGTGG TGATAGCCTG 
GTCCTGTACC TGTTCTCCGA TTCCATTGAG CTATGCAAGC GGCGTTCTAA AGGATTCAAC 
ACTGCAAAAT CGCCAAGCAC GGCCAAGACG CATAAGCATC TCAAGCTGAT ATCTTTGAAT 
ACGATCCGGT TAGTGATTGA CATCTCGGAT AGTCCAAGAG CTTTTGGTTT GCTCTTGCGG 
GGCGATAAGG AGAAGCTCTA TACGTTTACG ATCAGTGATG AGGAGACAGA TAAGTCGGTC 
TACGTAAAGA AATTGTGCAA CCAGATTGCA GCCCACACCT GCCGTACTGA TGCGGACAAA 
CTTTTGATTT GTCGCACCTC CCAGGAACTG GAGGTGGACA TAAGTGATGT GAATGTCAGC 
ACGCTCAGCA AAGCCTTTAA ACTGGCGGCC AAAACGCGAC TCAAGGTGGG TCGTGCTTTC 
TCCTTTAACA AAACGCCCAA CAAACTGAAA CGGGCCGTAT CCACGATGAT GACTTCGCCG 
TTTGGCAGCA CCAACTCTCT AACACCTGCC TCTCAATTGG CCCAGATGCG TTTGGCCAGC 
TGCACGAATA TAAATGAGGT TGATGATGAG GACTGTGCAA GCATGCGAAG CAGTTCGCCG 
TCAACACAAT CCGAGATGCT AGTGGTACCG CCACTTTCGG TGCAACCCAC GCGGAAAAAC 
AAGGCCGTTG TGGGCCGCAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246661.1
CDS	280..1983
Translation	MDYLFGDYLDSITNTPNTDRRDSLPISFNKRKRKRFSQRIQLEGTPLGSGKRRSSVSDAGLLSVSNSLFDCTTSPDKLESDKLLHAEPEASDATPAKKSMRFNHFMDFYTTESNYVGILDTILNLFKNKLEELAETNDPLLNKSEIKTIFGNFLPIHEVHQSMLEHLRKLHANWREDCLIGDIIIQHRDELIKAYPPYVNFFEQMKEQLQYCDREYPRFHAFLKINQTKPECGRQGLQDLMIRPVQRLPSISLLLNDILKHTTSGNADHGRLEEALKAIKQVTLHINEDKRRTESRMAIFDIFNDIEGCPAHLVSSNRSFILKCEVNELSDSLSGRGDSLVLYLFSDSIELCKRRSKGFNTAKSPSTAKTHKHLKLISLNTIRLVIDISDSPRAFGLLLRGDKEKLYTFTISDEETDKSVYVKKLCNQIAAHTCRTDADKLLICRTSQELEVDISDVNVSTLSKAFKLAAKTRLKVGRAFSFNKTPNKLKRAVSTMMTSPFGSTNSLTPASQLAQMRLASCTNINEVDDEDCASMRSSSPSTQSEMLVVPPLSVQPTRKNKAVVGRI

Target ORF information:

RefSeq Version	NM_001259732.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pebble (pbl), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259732.1

ORF Insert Sequence:

ATGGACTATC TGTTTGGCGA CTATTTGGAT AGCATCACGA ATACACCGAA CACAGATCGT 
CGGGATTCAT TGCCCATTAG CTTTAACAAA AGGAAACGCA AGCGTTTTTC GCAACGCATC 
CAGCTGGAGG GTACTCCGTT GGGTTCTGGT AAACGACGCT CCTCCGTTTC GGATGCCGGT 
CTATTGTCTG TCTCAAATAG TTTATTCGAT TGTACCACCA GTCCGGATAA ACTGGAGTCG 
GACAAGCTGT TGCATGCCGA GCCGGAGGCG AGCGATGCCA CTCCAGCCAA GAAATCGATG 
CGCTTTAATC ACTTTATGGA CTTTTATACC ACAGAGTCCA ACTACGTGGG CATATTGGAC 
ACCATACTGA ATCTTTTTAA AAACAAGTTG GAAGAGTTGG CTGAAACCAA TGATCCGCTG 
CTTAATAAGT CGGAGATCAA GACGATCTTT GGCAACTTCC TGCCTATCCA CGAGGTACAC 
CAAAGCATGC TGGAGCATCT GCGGAAATTG CATGCTAACT GGCGGGAGGA TTGTCTTATT 
GGAGACATTA TCATTCAACA TCGCGACGAG CTGATAAAGG CGTATCCACC ATACGTCAAC 
TTCTTCGAGC AGATGAAGGA GCAACTGCAG TACTGCGATC GGGAATATCC GCGTTTCCAC 
GCCTTTCTGA AGATTAATCA AACGAAACCG GAGTGCGGAC GCCAAGGTCT ACAAGATCTG 
ATGATCCGTC CAGTTCAGCG ATTGCCCAGT ATCAGTCTGC TGCTAAATGA TATATTGAAA 
CATACGACAA GTGGCAATGC GGACCACGGA CGTTTGGAGG AGGCTCTGAA GGCCATCAAG 
CAGGTGACAC TGCACATCAA CGAGGATAAA AGGCGGACCG AATCGCGCAT GGCCATCTTC 
GATATATTCA ACGATATCGA AGGATGTCCG GCGCATTTAG TGAGTTCCAA CCGCAGCTTT 
ATTTTGAAGT GTGAGGTGAA CGAGCTCTCC GATTCGCTGA GCGGTCGTGG TGATAGCCTG 
GTCCTGTACC TGTTCTCCGA TTCCATTGAG CTATGCAAGC GGCGTTCTAA AGGATTCAAC 
ACTGCAAAAT CGCCAAGCAC GGCCAAGACG CATAAGCATC TCAAGCTGAT ATCTTTGAAT 
ACGATCCGGT TAGTGATTGA CATCTCGGAT AGTCCAAGAG CTTTTGGTTT GCTCTTGCGG 
GGCGATAAGG AGAAGCTCTA TACGTTTACG ATCAGTGATG AGGAGACAGA TAAGTCGGTC 
TACGTAAAGA AATTGTGCAA CCAGATTGCA GCCCACACCT GCCGTACTGA TGCGGACAAA 
CTTTTGATTT GTCGCACCTC CCAGGAACTG GAGGTGGACA TAAGTGATGT GAATGTCAGC 
ACGCTCAGCA AAGCCTTTAA ACTGGCGGCC AAAACGCGAC TCAAGGTGGG TCGTGCTTTC 
TCCTTTAACA AAACGCCCAA CAAACTGAAA CGGGCCGTAT CCACGATGAT GACTTCGCCG 
TTTGGCAGCA CCAACTCTCT AACACCTGCC TCTCAATTGG CCCAGATGCG TTTGGCCAGC 
TGCACGAATA TAAATGAGGT TGATGATGAG GACTGTGCAA GCATGCGAAG CAGTTCGCCG 
TCAACACAAT CCGAGATGCT AGTGGTACCG CCACTTTCGG TGCAACCCAC GCGGAAAAAC 
AAGGCCGTTG TGGGCCGCAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pbl ( NM_168241.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729306.1 Drosophila melanogaster pebble (pbl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17840
Clone ID Related Accession (Same CDS sequence)	NM_168241.3
Accession Version	NM_168241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pebble, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_168241.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAATGG AGACCATTGA AGAGCAATCG AAGTGCGAGA TGTCCATAAC AACGCTGCCC 
ACGCGCATCT GCCTGGTGGG AGGCGTTGGC CAGGATGCGG ACACGCTGCA GGCAGCCGAA 
TCCTTTGGCC TGCCCATCGT TACGTCGGAC ACGGGTCTGG ACATTCTCGG AGAGTCGTCG 
GACTGGCGGA CATTCTACGT GCTGGATGAC TTCGAGGGTG CCAGCTTCGA GGCGATACAC 
AAGCAAAAGG AATGCATTCT GGGACCGCCG GCTTTGAAGT ACGCGGCCGA GATGAAACAG 
ACACTGGGCC AGAACTCGCG GCCCATTTAC AATTACGCGA TGCGCGGCGT GGTCACTTGT 
TTTACAGGCA TACGCAAGAA GGATGAGCTG ACAAAGCTGG TCAATCTCAT TCACTCGATG 
GGTGGCTGCA TCAAGAAGGA CCTGAACACG AAGACCACGC ATTTGATTTG CAACCACAGT 
GGCGGCGAAA AGTACCAGTA CGCCAAAACC TTTCGGTTGA CCGTGGTTCG TCCTGCTTGG 
GTGTTTGCCG CTTGGGCGGA TCGTAATTCG CTGGAGTTCG ATGCCACGCA AGAGAATTTC 
ACCAAGACGC ATCGCCTGAA GGCATTCGAG GGTCAAAAGA TTTGCTTCTT TGGATTCCCT 
GTGGAGGAGC ATCAGCATAT GGTGGATGTA CTTTTAGAAA ACGGAGGCGT TTGCGCCGAG 
CTCGATGATC CGGAGTGCTC GCATGTCGTG GTTGACGAGC ACACGACTTT GACGAAGCCG 
GAGCCGAAGA ATAATCACAC TCATATCCTG AAATCAGACT GGTTCTGGTA TACCATACAG 
AATGGCTATG CCAATGAGAT GGACTATCTG TTTGGCGACT ATTTGGATAG CATCACGAAT 
ACACCGAACA CAGATCGTCG GGATTCATTG CCCATTAGCT TTAACAAAAG GAAACGCAAG 
CGTTTTTCGC AACGCATCCA GCTGGAGGGT ACTCCGTTGG GTTCTGGTAA ACGACGCTCC 
TCCGTTTCGG ATGCCGGTCT ATTGTCTGTC TCAAATAGTT TATTCGATTG TACCACCAGT 
CCGGATAAAC TGGAGTCGGA CAAGCTGTTG CATGCCGAGC CGGAGGCGAG CGATGCCACT 
CCAGCCAAGA AATCGATGCG CTTTAATCAC TTTATGGACT TTTATACCAC AGAGTCCAAC 
TACGTGGGCA TATTGGACAC CATACTGAAT CTTTTTAAAA ACAAGTTGGA AGAGTTGGCT 
GAAACCAATG ATCCGCTGCT TAATAAGTCG GAGATCAAGA CGATCTTTGG CAACTTCCTG 
CCTATCCACG AGGTACACCA AAGCATGCTG GAGCATCTGC GGAAATTGCA TGCTAACTGG 
CGGGAGGATT GTCTTATTGG AGACATTATC ATTCAACATC GCGACGAGCT GATAAAGGCG 
TATCCACCAT ACGTCAACTT CTTCGAGCAG ATGAAGGAGC AACTGCAGTA CTGCGATCGG 
GAATATCCGC GTTTCCACGC CTTTCTGAAG ATTAATCAAA CGAAACCGGA GTGCGGACGC 
CAAGGTCTAC AAGATCTGAT GATCCGTCCA GTTCAGCGAT TGCCCAGTAT CAGTCTGCTG 
CTAAATGATA TATTGAAACA TACGACAAGT GGCAATGCGG ACCACGGACG TTTGGAGGAG 
GCTCTGAAGG CCATCAAGCA GGTGACACTG CACATCAACG AGGATAAAAG GCGGACCGAA 
TCGCGCATGG CCATCTTCGA TATATTCAAC GATATCGAAG GATGTCCGGC GCATTTAGTG 
AGTTCCAACC GCAGCTTTAT TTTGAAGTGT GAGGTGAACG AGCTCTCCGA TTCGCTGAGC 
GGTCGTGGTG ATAGCCTGGT CCTGTACCTG TTCTCCGATT CCATTGAGCT ATGCAAGCGG 
CGTTCTAAAG GATTCAACAC TGCAAAATCG CCAAGCACGG CCAAGACGCA TAAGCATCTC 
AAGCTGATAT CTTTGAATAC GATCCGGTTA GTGATTGACA TCTCGGATAG TCCAAGAGCT 
TTTGGTTTGC TCTTGCGGGG CGATAAGGAG AAGCTCTATA CGTTTACGAT CAGTGATGAG 
GAGACAGATA AGTCGGTCTA CGTAAAGAAA TTGTGCAACC AGATTGCAGC CCACACCTGC 
CGTACTGATG CGGACAAACT TTTGATTTGT CGCACCTCCC AGGAACTGGA GGTGGACATA 
AGTGATGTGA ATGTCAGCAC GCTCAGCAAA GCCTTTAAAC TGGCGGCCAA AACGCGACTC 
AAGGTGGGTC GTGCTTTCTC CTTTAACAAA ACGCCCAACA AACTGAAACG GGCCGTATCC 
ACGATGATGA CTTCGCCGTT TGGCAGCACC AACTCTCTAA CACCTGCCTC TCAATTGGCC 
CAGATGCGTT TGGCCAGCTG CACGAATATA AATGAGGTTG ATGATGAGGA CTGTGCAAGC 
ATGCGAAGCA GTTCGCCGTC AACACAATCC GAGATGCTAG TGGTACCGCC ACTTTCGGTG 
CAACCCACGC GGAAAAACAA GGCCGTTGTG GGCCGCATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729306.1
CDS	497..3058
Translation	MEMETIEEQSKCEMSITTLPTRICLVGGVGQDADTLQAAESFGLPIVTSDTGLDILGESSDWRTFYVLDDFEGASFEAIHKQKECILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRKKDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWADRNSLEFDATQENFTKTHRLKAFEGQKICFFGFPVEEHQHMVDVLLENGGVCAELDDPECSHVVVDEHTTLTKPEPKNNHTHILKSDWFWYTIQNGYANEMDYLFGDYLDSITNTPNTDRRDSLPISFNKRKRKRFSQRIQLEGTPLGSGKRRSSVSDAGLLSVSNSLFDCTTSPDKLESDKLLHAEPEASDATPAKKSMRFNHFMDFYTTESNYVGILDTILNLFKNKLEELAETNDPLLNKSEIKTIFGNFLPIHEVHQSMLEHLRKLHANWREDCLIGDIIIQHRDELIKAYPPYVNFFEQMKEQLQYCDREYPRFHAFLKINQTKPECGRQGLQDLMIRPVQRLPSISLLLNDILKHTTSGNADHGRLEEALKAIKQVTLHINEDKRRTESRMAIFDIFNDIEGCPAHLVSSNRSFILKCEVNELSDSLSGRGDSLVLYLFSDSIELCKRRSKGFNTAKSPSTAKTHKHLKLISLNTIRLVIDISDSPRAFGLLLRGDKEKLYTFTISDEETDKSVYVKKLCNQIAAHTCRTDADKLLICRTSQELEVDISDVNVSTLSKAFKLAAKTRLKVGRAFSFNKTPNKLKRAVSTMMTSPFGSTNSLTPASQLAQMRLASCTNINEVDDEDCASMRSSSPSTQSEMLVVPPLSVQPTRKNKAVVGRI

Target ORF information:

RefSeq Version	NM_168241.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pebble (pbl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168241.3

ORF Insert Sequence:

ATGGAAATGG AGACCATTGA AGAGCAATCG AAGTGCGAGA TGTCCATAAC AACGCTGCCC 
ACGCGCATCT GCCTGGTGGG AGGCGTTGGC CAGGATGCGG ACACGCTGCA GGCAGCCGAA 
TCCTTTGGCC TGCCCATCGT TACGTCGGAC ACGGGTCTGG ACATTCTCGG AGAGTCGTCG 
GACTGGCGGA CATTCTACGT GCTGGATGAC TTCGAGGGTG CCAGCTTCGA GGCGATACAC 
AAGCAAAAGG AATGCATTCT GGGACCGCCG GCTTTGAAGT ACGCGGCCGA GATGAAACAG 
ACACTGGGCC AGAACTCGCG GCCCATTTAC AATTACGCGA TGCGCGGCGT GGTCACTTGT 
TTTACAGGCA TACGCAAGAA GGATGAGCTG ACAAAGCTGG TCAATCTCAT TCACTCGATG 
GGTGGCTGCA TCAAGAAGGA CCTGAACACG AAGACCACGC ATTTGATTTG CAACCACAGT 
GGCGGCGAAA AGTACCAGTA CGCCAAAACC TTTCGGTTGA CCGTGGTTCG TCCTGCTTGG 
GTGTTTGCCG CTTGGGCGGA TCGTAATTCG CTGGAGTTCG ATGCCACGCA AGAGAATTTC 
ACCAAGACGC ATCGCCTGAA GGCATTCGAG GGTCAAAAGA TTTGCTTCTT TGGATTCCCT 
GTGGAGGAGC ATCAGCATAT GGTGGATGTA CTTTTAGAAA ACGGAGGCGT TTGCGCCGAG 
CTCGATGATC CGGAGTGCTC GCATGTCGTG GTTGACGAGC ACACGACTTT GACGAAGCCG 
GAGCCGAAGA ATAATCACAC TCATATCCTG AAATCAGACT GGTTCTGGTA TACCATACAG 
AATGGCTATG CCAATGAGAT GGACTATCTG TTTGGCGACT ATTTGGATAG CATCACGAAT 
ACACCGAACA CAGATCGTCG GGATTCATTG CCCATTAGCT TTAACAAAAG GAAACGCAAG 
CGTTTTTCGC AACGCATCCA GCTGGAGGGT ACTCCGTTGG GTTCTGGTAA ACGACGCTCC 
TCCGTTTCGG ATGCCGGTCT ATTGTCTGTC TCAAATAGTT TATTCGATTG TACCACCAGT 
CCGGATAAAC TGGAGTCGGA CAAGCTGTTG CATGCCGAGC CGGAGGCGAG CGATGCCACT 
CCAGCCAAGA AATCGATGCG CTTTAATCAC TTTATGGACT TTTATACCAC AGAGTCCAAC 
TACGTGGGCA TATTGGACAC CATACTGAAT CTTTTTAAAA ACAAGTTGGA AGAGTTGGCT 
GAAACCAATG ATCCGCTGCT TAATAAGTCG GAGATCAAGA CGATCTTTGG CAACTTCCTG 
CCTATCCACG AGGTACACCA AAGCATGCTG GAGCATCTGC GGAAATTGCA TGCTAACTGG 
CGGGAGGATT GTCTTATTGG AGACATTATC ATTCAACATC GCGACGAGCT GATAAAGGCG 
TATCCACCAT ACGTCAACTT CTTCGAGCAG ATGAAGGAGC AACTGCAGTA CTGCGATCGG 
GAATATCCGC GTTTCCACGC CTTTCTGAAG ATTAATCAAA CGAAACCGGA GTGCGGACGC 
CAAGGTCTAC AAGATCTGAT GATCCGTCCA GTTCAGCGAT TGCCCAGTAT CAGTCTGCTG 
CTAAATGATA TATTGAAACA TACGACAAGT GGCAATGCGG ACCACGGACG TTTGGAGGAG 
GCTCTGAAGG CCATCAAGCA GGTGACACTG CACATCAACG AGGATAAAAG GCGGACCGAA 
TCGCGCATGG CCATCTTCGA TATATTCAAC GATATCGAAG GATGTCCGGC GCATTTAGTG 
AGTTCCAACC GCAGCTTTAT TTTGAAGTGT GAGGTGAACG AGCTCTCCGA TTCGCTGAGC 
GGTCGTGGTG ATAGCCTGGT CCTGTACCTG TTCTCCGATT CCATTGAGCT ATGCAAGCGG 
CGTTCTAAAG GATTCAACAC TGCAAAATCG CCAAGCACGG CCAAGACGCA TAAGCATCTC 
AAGCTGATAT CTTTGAATAC GATCCGGTTA GTGATTGACA TCTCGGATAG TCCAAGAGCT 
TTTGGTTTGC TCTTGCGGGG CGATAAGGAG AAGCTCTATA CGTTTACGAT CAGTGATGAG 
GAGACAGATA AGTCGGTCTA CGTAAAGAAA TTGTGCAACC AGATTGCAGC CCACACCTGC 
CGTACTGATG CGGACAAACT TTTGATTTGT CGCACCTCCC AGGAACTGGA GGTGGACATA 
AGTGATGTGA ATGTCAGCAC GCTCAGCAAA GCCTTTAAAC TGGCGGCCAA AACGCGACTC 
AAGGTGGGTC GTGCTTTCTC CTTTAACAAA ACGCCCAACA AACTGAAACG GGCCGTATCC 
ACGATGATGA CTTCGCCGTT TGGCAGCACC AACTCTCTAA CACCTGCCTC TCAATTGGCC 
CAGATGCGTT TGGCCAGCTG CACGAATATA AATGAGGTTG ATGATGAGGA CTGTGCAAGC 
ATGCGAAGCA GTTCGCCGTC AACACAATCC GAGATGCTAG TGGTACCGCC ACTTTCGGTG 
CAACCCACGC GGAAAAACAA GGCCGTTGTG GGCCGCATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pbl ( NM_001104070.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097540.1 Drosophila melanogaster pebble (pbl), transcript variant E, partial mRNA.

Price & Availability↑

CloneID	OFa17842
Clone ID Related Accession (Same CDS sequence)	NM_001104070.2
Accession Version	NM_001104070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pebble, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_001104070.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGTTACACT TACCGCTGGT GCAGTTCAAC TTTGGCAACC ATTTGGGCAA AAAGAGCAAC 
AAAGTGGTCA CGGTTCAGCG ACAATCCGCC TCCTATTTCA CATATGCCGC CGACGAGGAG 
GTCAAACAGG CGAAGCTTCG ACAGGCCGCC TTGTGTCAGG TGTACGAGGA TTTTACAGAG 
ATGTCCATAA CAACGCTGCC CACGCGCATC TGCCTGGTGG GAGGCGTTGG CCAGGATGCG 
GACACGCTGC AGGCAGCCGA ATCCTTTGGC CTGCCCATCG TTACGTCGGA CACGGGTCTG 
GACATTCTCG GAGAGTCGTC GGACTGGCGG ACATTCTACG TGCTGGATGA CTTCGAGGGT 
GCCAGCTTCG AGGCGATACA CAAGCAAAAG GAATGCATTC TGGGACCGCC GGCTTTGAAG 
TACGCGGCCG AGATGAAACA GACACTGGGC CAGAACTCGC GGCCCATTTA CAATTACGCG 
ATGCGCGGCG TGGTCACTTG TTTTACAGGC ATACGCAAGA AGGATGAGCT GACAAAGCTG 
GTCAATCTCA TTCACTCGAT GGGTGGCTGC ATCAAGAAGG ACCTGAACAC GAAGACCACG 
CATTTGATTT GCAACCACAG TGGCGGCGAA AAGTACCAGT ACGCCAAAAC CTTTCGGTTG 
ACCGTGGTTC GTCCTGCTTG GGTGTTTGCC GCTTGGGCGG ATCGTAATTC GCTGGAGTTC 
GATGCCACGC AAGAGAATTT CACCAAGACG CATCGCCTGA AGGCATTCGA GGGTCAAAAG 
ATTTGCTTCT TTGGATTCCC TGTGGAGGAG CATCAGCATA TGGTGGATGT ACTTTTAGAA 
AACGGAGGCG TTTGCGCCGA GCTCGATGAT CCGGAGTGCT CGCATGTCGT GGTTGACGAG 
CACACGACTT TGACGAAGCC GGAGCCGAAG AATAATCACA CTCATATCCT GAAATCAGAC 
TGGTTCTGGT ATACCATACA GAATGGCTAT GCCAATGAGA TGGACTATCT GTTTGGCGAC 
TATTTGGATA GCATCACGAA TACACCGAAC ACAGATCGTC GGGATTCATT GCCCATTAGC 
TTTAACAAAA GGAAACGCAA GCGTTTTTCG CAACGCATCC AGCTGGAGGG TACTCCGTTG 
GGTTCTGGTA AACGACGCTC CTCCGTTTCG GATGCCGGTC TATTGTCTGT CTCAAATAGT 
TTATTCGATT GTACCACCAG TCCGGATAAA CTGGAGTCGG ACAAGCTGTT GCATGCCGAG 
CCGGAGGCGA GCGATGCCAC TCCAGCCAAG AAATCGATGC GCTTTAATCA CTTTATGGAC 
TTTTATACCA CAGAGTCCAA CTACGTGGGC ATATTGGACA CCATACTGAA TCTTTTTAAA 
AACAAGTTGG AAGAGTTGGC TGAAACCAAT GATCCGCTGC TTAATAAGTC GGAGATCAAG 
ACGATCTTTG GCAACTTCCT GCCTATCCAC GAGGTACACC AAAGCATGCT GGAGCATCTG 
CGGAAATTGC ATGCTAACTG GCGGGAGGAT TGTCTTATTG GAGACATTAT CATTCAACAT 
CGCGACGAGC TGATAAAGGC GTATCCACCA TACGTCAACT TCTTCGAGCA GATGAAGGAG 
CAACTGCAGT ACTGCGATCG GGAATATCCG CGTTTCCACG CCTTTCTGAA GATTAATCAA 
ACGAAACCGG AGTGCGGACG CCAAGGTCTA CAAGATCTGA TGATCCGTCC AGTTCAGCGA 
TTGCCCAGTA TCAGTCTGCT GCTAAATGAT ATATTGAAAC ATACGACAAG TGGCAATGCG 
GACCACGGAC GTTTGGAGGA GGCTCTGAAG GCCATCAAGC AGGTGACACT GCACATCAAC 
GAGGATAAAA GGCGGACCGA ATCGCGCATG GCCATCTTCG ATATATTCAA CGATATCGAA 
GGATGTCCGG CGCATTTAGT GAGTTCCAAC CGCAGCTTTA TTTTGAAGTG TGAGGTGAAC 
GAGCTCTCCG ATTCGCTGAG CGGTCGTGGT GATAGCCTGG TCCTGTACCT GTTCTCCGAT 
TCCATTGAGC TATGCAAGCG GCGTTCTAAA GGATTCAACA CTGCAAAATC GCCAAGCACG 
GCCAAGACGC ATAAGCATCT CAAGCTGATA TCTTTGAATA CGATCCGGTT AGTGATTGAC 
ATCTCGGATA GTCCAAGAGC TTTTGGTTTG CTCTTGCGGG GCGATAAGGA GAAGCTCTAT 
ACGTTTACGA TCAGTGATGA GGAGACAGAT AAGTCGGTCT ACGTAAAGAA ATTGTGCAAC 
CAGATTGCAG CCCACACCTG CCGTACTGAT GCGGACAAAC TTTTGATTTG TCGCACCTCC 
CAGGAACTGG AGGTGGACAT AAGTGATGTG AATGTCAGCA CGCTCAGCAA AGCCTTTAAA 
CTGGCGGCCA AAACGCGACT CAAGGTGGGT CGTGCTTTCT CCTTTAACAA AACGCCCAAC 
AAACTGAAAC GGGCCGTATC CACGATGATG ACTTCGCCGT TTGGCAGCAC CAACTCTCTA 
ACACCTGCCT CTCAATTGGC CCAGATGCGT TTGGCCAGCT GCACGAATAT AAATGAGGTT 
GATGATGAGG ACTGTGCAAG CATGCGAAGC AGTTCGCCGT CAACACAATC CGAGATGCTA 
GTGGTACCGC CACTTTCGGT GCAACCCACG CGGAAAAACA AGGCCGTTGT GGGCCGCATT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097540.1
CDS	1..2703
Translation	MLHLPLVQFNFGNHLGKKSNKVVTVQRQSASYFTYAADEEVKQAKLRQAALCQVYEDFTEMSITTLPTRICLVGGVGQDADTLQAAESFGLPIVTSDTGLDILGESSDWRTFYVLDDFEGASFEAIHKQKECILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRKKDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWADRNSLEFDATQENFTKTHRLKAFEGQKICFFGFPVEEHQHMVDVLLENGGVCAELDDPECSHVVVDEHTTLTKPEPKNNHTHILKSDWFWYTIQNGYANEMDYLFGDYLDSITNTPNTDRRDSLPISFNKRKRKRFSQRIQLEGTPLGSGKRRSSVSDAGLLSVSNSLFDCTTSPDKLESDKLLHAEPEASDATPAKKSMRFNHFMDFYTTESNYVGILDTILNLFKNKLEELAETNDPLLNKSEIKTIFGNFLPIHEVHQSMLEHLRKLHANWREDCLIGDIIIQHRDELIKAYPPYVNFFEQMKEQLQYCDREYPRFHAFLKINQTKPECGRQGLQDLMIRPVQRLPSISLLLNDILKHTTSGNADHGRLEEALKAIKQVTLHINEDKRRTESRMAIFDIFNDIEGCPAHLVSSNRSFILKCEVNELSDSLSGRGDSLVLYLFSDSIELCKRRSKGFNTAKSPSTAKTHKHLKLISLNTIRLVIDISDSPRAFGLLLRGDKEKLYTFTISDEETDKSVYVKKLCNQIAAHTCRTDADKLLICRTSQELEVDISDVNVSTLSKAFKLAAKTRLKVGRAFSFNKTPNKLKRAVSTMMTSPFGSTNSLTPASQLAQMRLASCTNINEVDDEDCASMRSSSPSTQSEMLVVPPLSVQPTRKNKAVVGRI

Target ORF information:

RefSeq Version	NM_001104070.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pebble (pbl), transcript variant E, partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104070.2

ORF Insert Sequence:

ATGTTACACT TACCGCTGGT GCAGTTCAAC TTTGGCAACC ATTTGGGCAA AAAGAGCAAC 
AAAGTGGTCA CGGTTCAGCG ACAATCCGCC TCCTATTTCA CATATGCCGC CGACGAGGAG 
GTCAAACAGG CGAAGCTTCG ACAGGCCGCC TTGTGTCAGG TGTACGAGGA TTTTACAGAG 
ATGTCCATAA CAACGCTGCC CACGCGCATC TGCCTGGTGG GAGGCGTTGG CCAGGATGCG 
GACACGCTGC AGGCAGCCGA ATCCTTTGGC CTGCCCATCG TTACGTCGGA CACGGGTCTG 
GACATTCTCG GAGAGTCGTC GGACTGGCGG ACATTCTACG TGCTGGATGA CTTCGAGGGT 
GCCAGCTTCG AGGCGATACA CAAGCAAAAG GAATGCATTC TGGGACCGCC GGCTTTGAAG 
TACGCGGCCG AGATGAAACA GACACTGGGC CAGAACTCGC GGCCCATTTA CAATTACGCG 
ATGCGCGGCG TGGTCACTTG TTTTACAGGC ATACGCAAGA AGGATGAGCT GACAAAGCTG 
GTCAATCTCA TTCACTCGAT GGGTGGCTGC ATCAAGAAGG ACCTGAACAC GAAGACCACG 
CATTTGATTT GCAACCACAG TGGCGGCGAA AAGTACCAGT ACGCCAAAAC CTTTCGGTTG 
ACCGTGGTTC GTCCTGCTTG GGTGTTTGCC GCTTGGGCGG ATCGTAATTC GCTGGAGTTC 
GATGCCACGC AAGAGAATTT CACCAAGACG CATCGCCTGA AGGCATTCGA GGGTCAAAAG 
ATTTGCTTCT TTGGATTCCC TGTGGAGGAG CATCAGCATA TGGTGGATGT ACTTTTAGAA 
AACGGAGGCG TTTGCGCCGA GCTCGATGAT CCGGAGTGCT CGCATGTCGT GGTTGACGAG 
CACACGACTT TGACGAAGCC GGAGCCGAAG AATAATCACA CTCATATCCT GAAATCAGAC 
TGGTTCTGGT ATACCATACA GAATGGCTAT GCCAATGAGA TGGACTATCT GTTTGGCGAC 
TATTTGGATA GCATCACGAA TACACCGAAC ACAGATCGTC GGGATTCATT GCCCATTAGC 
TTTAACAAAA GGAAACGCAA GCGTTTTTCG CAACGCATCC AGCTGGAGGG TACTCCGTTG 
GGTTCTGGTA AACGACGCTC CTCCGTTTCG GATGCCGGTC TATTGTCTGT CTCAAATAGT 
TTATTCGATT GTACCACCAG TCCGGATAAA CTGGAGTCGG ACAAGCTGTT GCATGCCGAG 
CCGGAGGCGA GCGATGCCAC TCCAGCCAAG AAATCGATGC GCTTTAATCA CTTTATGGAC 
TTTTATACCA CAGAGTCCAA CTACGTGGGC ATATTGGACA CCATACTGAA TCTTTTTAAA 
AACAAGTTGG AAGAGTTGGC TGAAACCAAT GATCCGCTGC TTAATAAGTC GGAGATCAAG 
ACGATCTTTG GCAACTTCCT GCCTATCCAC GAGGTACACC AAAGCATGCT GGAGCATCTG 
CGGAAATTGC ATGCTAACTG GCGGGAGGAT TGTCTTATTG GAGACATTAT CATTCAACAT 
CGCGACGAGC TGATAAAGGC GTATCCACCA TACGTCAACT TCTTCGAGCA GATGAAGGAG 
CAACTGCAGT ACTGCGATCG GGAATATCCG CGTTTCCACG CCTTTCTGAA GATTAATCAA 
ACGAAACCGG AGTGCGGACG CCAAGGTCTA CAAGATCTGA TGATCCGTCC AGTTCAGCGA 
TTGCCCAGTA TCAGTCTGCT GCTAAATGAT ATATTGAAAC ATACGACAAG TGGCAATGCG 
GACCACGGAC GTTTGGAGGA GGCTCTGAAG GCCATCAAGC AGGTGACACT GCACATCAAC 
GAGGATAAAA GGCGGACCGA ATCGCGCATG GCCATCTTCG ATATATTCAA CGATATCGAA 
GGATGTCCGG CGCATTTAGT GAGTTCCAAC CGCAGCTTTA TTTTGAAGTG TGAGGTGAAC 
GAGCTCTCCG ATTCGCTGAG CGGTCGTGGT GATAGCCTGG TCCTGTACCT GTTCTCCGAT 
TCCATTGAGC TATGCAAGCG GCGTTCTAAA GGATTCAACA CTGCAAAATC GCCAAGCACG 
GCCAAGACGC ATAAGCATCT CAAGCTGATA TCTTTGAATA CGATCCGGTT AGTGATTGAC 
ATCTCGGATA GTCCAAGAGC TTTTGGTTTG CTCTTGCGGG GCGATAAGGA GAAGCTCTAT 
ACGTTTACGA TCAGTGATGA GGAGACAGAT AAGTCGGTCT ACGTAAAGAA ATTGTGCAAC 
CAGATTGCAG CCCACACCTG CCGTACTGAT GCGGACAAAC TTTTGATTTG TCGCACCTCC 
CAGGAACTGG AGGTGGACAT AAGTGATGTG AATGTCAGCA CGCTCAGCAA AGCCTTTAAA 
CTGGCGGCCA AAACGCGACT CAAGGTGGGT CGTGCTTTCT CCTTTAACAA AACGCCCAAC 
AAACTGAAAC GGGCCGTATC CACGATGATG ACTTCGCCGT TTGGCAGCAC CAACTCTCTA 
ACACCTGCCT CTCAATTGGC CCAGATGCGT TTGGCCAGCT GCACGAATAT AAATGAGGTT 
GATGATGAGG ACTGTGCAAG CATGCGAAGC AGTTCGCCGT CAACACAATC CGAGATGCTA 
GTGGTACCGC CACTTTCGGT GCAACCCACG CGGAAAAACA AGGCCGTTGT GGGCCGCATT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pbl ( NM_168242.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729307.1 Drosophila melanogaster pebble (pbl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa17843
Clone ID Related Accession (Same CDS sequence)	NM_168242.3
Accession Version	NM_168242.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pebble, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_168242.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAG GGGAGAAAAC GGCAAAAGGA AGATGGAAAC TCATTCTGGG ACCGCCGGCT 
TTGAAGTACG CGGCCGAGAT GAAACAGACA CTGGGCCAGA ACTCGCGGCC CATTTACAAT 
TACGCGATGC GCGGCGTGGT CACTTGTTTT ACAGGCATAC GCAAGAAGGA TGAGCTGACA 
AAGCTGGTCA ATCTCATTCA CTCGATGGGT GGCTGCATCA AGAAGGACCT GAACACGAAG 
ACCACGCATT TGATTTGCAA CCACAGTGGC GGCGAAAAGT ACCAGTACGC CAAAACCTTT 
CGGTTGACCG TGGTTCGTCC TGCTTGGGTG TTTGCCGCTT GGGCGGATCG TAATTCGCTG 
GAGTTCGATG CCACGCAAGA GAATTTCACC AAGACGCATC GCCTGAAGGC ATTCGAGGGT 
CAAAAGATTT GCTTCTTTGG ATTCCCTGTG GAGGAGCATC AGCATATGGT GGATGTACTT 
TTAGAAAACG GAGGCGTTTG CGCCGAGCTC GATGATCCGG AGTGCTCGCA TGTCGTGGTT 
GACGAGCACA CGACTTTGAC GAAGCCGGAG CCGAAGAATA ATCACACTCA TATCCTGAAA 
TCAGACTGGT TCTGGTATAC CATACAGAAT GGCTATGCCA ATGAGATGGA CTATCTGTTT 
GGCGACTATT TGGATAGCAT CACGAATACA CCGAACACAG ATCGTCGGGA TTCATTGCCC 
ATTAGCTTTA ACAAAAGGAA ACGCAAGCGT TTTTCGCAAC GCATCCAGCT GGAGGGTACT 
CCGTTGGGTT CTGGTAAACG ACGCTCCTCC GTTTCGGATG CCGGTCTATT GTCTGTCTCA 
AATAGTTTAT TCGATTGTAC CACCAGTCCG GATAAACTGG AGTCGGACAA GCTGTTGCAT 
GCCGAGCCGG AGGCGAGCGA TGCCACTCCA GCCAAGAAAT CGATGCGCTT TAATCACTTT 
ATGGACTTTT ATACCACAGA GTCCAACTAC GTGGGCATAT TGGACACCAT ACTGAATCTT 
TTTAAAAACA AGTTGGAAGA GTTGGCTGAA ACCAATGATC CGCTGCTTAA TAAGTCGGAG 
ATCAAGACGA TCTTTGGCAA CTTCCTGCCT ATCCACGAGG TACACCAAAG CATGCTGGAG 
CATCTGCGGA AATTGCATGC TAACTGGCGG GAGGATTGTC TTATTGGAGA CATTATCATT 
CAACATCGCG ACGAGCTGAT AAAGGCGTAT CCACCATACG TCAACTTCTT CGAGCAGATG 
AAGGAGCAAC TGCAGTACTG CGATCGGGAA TATCCGCGTT TCCACGCCTT TCTGAAGATT 
AATCAAACGA AACCGGAGTG CGGACGCCAA GGTCTACAAG ATCTGATGAT CCGTCCAGTT 
CAGCGATTGC CCAGTATCAG TCTGCTGCTA AATGATATAT TGAAACATAC GACAAGTGGC 
AATGCGGACC ACGGACGTTT GGAGGAGGCT CTGAAGGCCA TCAAGCAGGT GACACTGCAC 
ATCAACGAGG ATAAAAGGCG GACCGAATCG CGCATGGCCA TCTTCGATAT ATTCAACGAT 
ATCGAAGGAT GTCCGGCGCA TTTAGTGAGT TCCAACCGCA GCTTTATTTT GAAGTGTGAG 
GTGAACGAGC TCTCCGATTC GCTGAGCGGT CGTGGTGATA GCCTGGTCCT GTACCTGTTC 
TCCGATTCCA TTGAGCTATG CAAGCGGCGT TCTAAAGGAT TCAACACTGC AAAATCGCCA 
AGCACGGCCA AGACGCATAA GCATCTCAAG CTGATATCTT TGAATACGAT CCGGTTAGTG 
ATTGACATCT CGGATAGTCC AAGAGCTTTT GGTTTGCTCT TGCGGGGCGA TAAGGAGAAG 
CTCTATACGT TTACGATCAG TGATGAGGAG ACAGATAAGT CGGTCTACGT AAAGAAATTG 
TGCAACCAGA TTGCAGCCCA CACCTGCCGT ACTGATGCGG ACAAACTTTT GATTTGTCGC 
ACCTCCCAGG AACTGGAGGT GGACATAAGT GATGTGAATG TCAGCACGCT CAGCAAAGCC 
TTTAAACTGG CGGCCAAAAC GCGACTCAAG GTGGGTCGTG CTTTCTCCTT TAACAAAACG 
CCCAACAAAC TGAAACGGGC CGTATCCACG ATGATGACTT CGCCGTTTGG CAGCACCAAC 
TCTCTAACAC CTGCCTCTCA ATTGGCCCAG ATGCGTTTGG CCAGCTGCAC GAATATAAAT 
GAGGTTGATG ATGAGGACTG TGCAAGCATG CGAAGCAGTT CGCCGTCAAC ACAATCCGAG 
ATGCTAGTGG TACCGCCACT TTCGGTGCAA CCCACGCGGA AAAACAAGGC CGTTGTGGGC 
CGCATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729307.1
CDS	348..2696
Translation	MESGEKTAKGRWKLILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRKKDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWADRNSLEFDATQENFTKTHRLKAFEGQKICFFGFPVEEHQHMVDVLLENGGVCAELDDPECSHVVVDEHTTLTKPEPKNNHTHILKSDWFWYTIQNGYANEMDYLFGDYLDSITNTPNTDRRDSLPISFNKRKRKRFSQRIQLEGTPLGSGKRRSSVSDAGLLSVSNSLFDCTTSPDKLESDKLLHAEPEASDATPAKKSMRFNHFMDFYTTESNYVGILDTILNLFKNKLEELAETNDPLLNKSEIKTIFGNFLPIHEVHQSMLEHLRKLHANWREDCLIGDIIIQHRDELIKAYPPYVNFFEQMKEQLQYCDREYPRFHAFLKINQTKPECGRQGLQDLMIRPVQRLPSISLLLNDILKHTTSGNADHGRLEEALKAIKQVTLHINEDKRRTESRMAIFDIFNDIEGCPAHLVSSNRSFILKCEVNELSDSLSGRGDSLVLYLFSDSIELCKRRSKGFNTAKSPSTAKTHKHLKLISLNTIRLVIDISDSPRAFGLLLRGDKEKLYTFTISDEETDKSVYVKKLCNQIAAHTCRTDADKLLICRTSQELEVDISDVNVSTLSKAFKLAAKTRLKVGRAFSFNKTPNKLKRAVSTMMTSPFGSTNSLTPASQLAQMRLASCTNINEVDDEDCASMRSSSPSTQSEMLVVPPLSVQPTRKNKAVVGRI

Target ORF information:

RefSeq Version	NM_168242.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pebble (pbl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168242.3

ORF Insert Sequence:

ATGGAGTCAG GGGAGAAAAC GGCAAAAGGA AGATGGAAAC TCATTCTGGG ACCGCCGGCT 
TTGAAGTACG CGGCCGAGAT GAAACAGACA CTGGGCCAGA ACTCGCGGCC CATTTACAAT 
TACGCGATGC GCGGCGTGGT CACTTGTTTT ACAGGCATAC GCAAGAAGGA TGAGCTGACA 
AAGCTGGTCA ATCTCATTCA CTCGATGGGT GGCTGCATCA AGAAGGACCT GAACACGAAG 
ACCACGCATT TGATTTGCAA CCACAGTGGC GGCGAAAAGT ACCAGTACGC CAAAACCTTT 
CGGTTGACCG TGGTTCGTCC TGCTTGGGTG TTTGCCGCTT GGGCGGATCG TAATTCGCTG 
GAGTTCGATG CCACGCAAGA GAATTTCACC AAGACGCATC GCCTGAAGGC ATTCGAGGGT 
CAAAAGATTT GCTTCTTTGG ATTCCCTGTG GAGGAGCATC AGCATATGGT GGATGTACTT 
TTAGAAAACG GAGGCGTTTG CGCCGAGCTC GATGATCCGG AGTGCTCGCA TGTCGTGGTT 
GACGAGCACA CGACTTTGAC GAAGCCGGAG CCGAAGAATA ATCACACTCA TATCCTGAAA 
TCAGACTGGT TCTGGTATAC CATACAGAAT GGCTATGCCA ATGAGATGGA CTATCTGTTT 
GGCGACTATT TGGATAGCAT CACGAATACA CCGAACACAG ATCGTCGGGA TTCATTGCCC 
ATTAGCTTTA ACAAAAGGAA ACGCAAGCGT TTTTCGCAAC GCATCCAGCT GGAGGGTACT 
CCGTTGGGTT CTGGTAAACG ACGCTCCTCC GTTTCGGATG CCGGTCTATT GTCTGTCTCA 
AATAGTTTAT TCGATTGTAC CACCAGTCCG GATAAACTGG AGTCGGACAA GCTGTTGCAT 
GCCGAGCCGG AGGCGAGCGA TGCCACTCCA GCCAAGAAAT CGATGCGCTT TAATCACTTT 
ATGGACTTTT ATACCACAGA GTCCAACTAC GTGGGCATAT TGGACACCAT ACTGAATCTT 
TTTAAAAACA AGTTGGAAGA GTTGGCTGAA ACCAATGATC CGCTGCTTAA TAAGTCGGAG 
ATCAAGACGA TCTTTGGCAA CTTCCTGCCT ATCCACGAGG TACACCAAAG CATGCTGGAG 
CATCTGCGGA AATTGCATGC TAACTGGCGG GAGGATTGTC TTATTGGAGA CATTATCATT 
CAACATCGCG ACGAGCTGAT AAAGGCGTAT CCACCATACG TCAACTTCTT CGAGCAGATG 
AAGGAGCAAC TGCAGTACTG CGATCGGGAA TATCCGCGTT TCCACGCCTT TCTGAAGATT 
AATCAAACGA AACCGGAGTG CGGACGCCAA GGTCTACAAG ATCTGATGAT CCGTCCAGTT 
CAGCGATTGC CCAGTATCAG TCTGCTGCTA AATGATATAT TGAAACATAC GACAAGTGGC 
AATGCGGACC ACGGACGTTT GGAGGAGGCT CTGAAGGCCA TCAAGCAGGT GACACTGCAC 
ATCAACGAGG ATAAAAGGCG GACCGAATCG CGCATGGCCA TCTTCGATAT ATTCAACGAT 
ATCGAAGGAT GTCCGGCGCA TTTAGTGAGT TCCAACCGCA GCTTTATTTT GAAGTGTGAG 
GTGAACGAGC TCTCCGATTC GCTGAGCGGT CGTGGTGATA GCCTGGTCCT GTACCTGTTC 
TCCGATTCCA TTGAGCTATG CAAGCGGCGT TCTAAAGGAT TCAACACTGC AAAATCGCCA 
AGCACGGCCA AGACGCATAA GCATCTCAAG CTGATATCTT TGAATACGAT CCGGTTAGTG 
ATTGACATCT CGGATAGTCC AAGAGCTTTT GGTTTGCTCT TGCGGGGCGA TAAGGAGAAG 
CTCTATACGT TTACGATCAG TGATGAGGAG ACAGATAAGT CGGTCTACGT AAAGAAATTG 
TGCAACCAGA TTGCAGCCCA CACCTGCCGT ACTGATGCGG ACAAACTTTT GATTTGTCGC 
ACCTCCCAGG AACTGGAGGT GGACATAAGT GATGTGAATG TCAGCACGCT CAGCAAAGCC 
TTTAAACTGG CGGCCAAAAC GCGACTCAAG GTGGGTCGTG CTTTCTCCTT TAACAAAACG 
CCCAACAAAC TGAAACGGGC CGTATCCACG ATGATGACTT CGCCGTTTGG CAGCACCAAC 
TCTCTAACAC CTGCCTCTCA ATTGGCCCAG ATGCGTTTGG CCAGCTGCAC GAATATAAAT 
GAGGTTGATG ATGAGGACTG TGCAAGCATG CGAAGCAGTT CGCCGTCAAC ACAATCCGAG 
ATGCTAGTGG TACCGCCACT TTCGGTGCAA CCCACGCGGA AAAACAAGGC CGTTGTGGGC 
CGCATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pbl ( NM_079241.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523965.2 Drosophila melanogaster pebble (pbl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17841
Clone ID Related Accession (Same CDS sequence)	NM_079241.4
Accession Version	NM_079241.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pebble, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_079241.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAATGG AGACCATTGA AGAGCAATCG AAGTGCGAGA TGTCCATAAC AACGCTGCCC 
ACGCGCATCT GCCTGGTGGG AGGCGTTGGC CAGGATGCGG ACACGCTGCA GGCAGCCGAA 
TCCTTTGGCC TGCCCATCGT TACGTCGGAC ACGGGTCTGG ACATTCTCGG AGAGTCGTCG 
GACTGGCGGA CATTCTACGT GCTGGATGAC TTCGAGGGTG CCAGCTTCGA GGCGATACAC 
AAGCAAAAGG AATGCATTCT GGGACCGCCG GCTTTGAAGT ACGCGGCCGA GATGAAACAG 
ACACTGGGCC AGAACTCGCG GCCCATTTAC AATTACGCGA TGCGCGGCGT GGTCACTTGT 
TTTACAGGCA TACGCAAGAA GGATGAGCTG ACAAAGCTGG TCAATCTCAT TCACTCGATG 
GGTGGCTGCA TCAAGAAGGA CCTGAACACG AAGACCACGC ATTTGATTTG CAACCACAGT 
GGCGGCGAAA AGTACCAGTA CGCCAAAACC TTTCGGTTGA CCGTGGTTCG TCCTGCTTGG 
GTGTTTGCCG CTTGGGCGGA TCGTAATTCG CTGGAGTTCG ATGCCACGCA AGAGAATTTC 
ACCAAGACGC ATCGCCTGAA GGCATTCGAG GGTCAAAAGA TTTGCTTCTT TGGATTCCCT 
GTGGAGGAGC ATCAGCATAT GGTGGATGTA CTTTTAGAAA ACGGAGGCGT TTGCGCCGAG 
CTCGATGATC CGGAGTGCTC GCATGTCGTG ATGCCCAATA CGGGCGCAGT ATTTACAAGC 
ACTAGCACTA GCACAGATAC TAACCCAAGC ACCCTAATTA ACCCACCCGA ATCCCAAGTC 
CCAGGCAGTA GTAGTGCATC GCAGGCGGGG GAAGAAGAGC AACGAAAGGC TGAGCATCTT 
GAGCAGGCGG AAATCAAAAA GATGGAATAC TCACACGCGG AAACGGACAA TGGAATGAGT 
TTGGACATTG ACCAGGAGCA AAAAGAGAAT CAAGGCATTG AAGACGAAGA TGACATACAC 
TTTGACGAAC GCCTGTTTGT GGAGGCGGCC GATATTATAA ACCACGGAGA AGACCAATTG 
CCTGAATCTG ATGAAGAAGA CCTGGAGGAG GAGCAGAAAC CCAAGGTCGA GGAGGTTCTA 
AAAGAACAGG AAGTTGAGGA GGATGTGCAA GCCAGTACGC CAAAGACTAG TAAAATAAGA 
ACTAGCTTAA AGGCTGCCAC GCCTCTTAGC TCACCCGAAA TGGATGGCAT TGCTTTGGAC 
TTCGAGAGCA CCACCCAGAA CATTTCACCC ATTTTAAAAG CTATTGACGA GTGCGACTTA 
GAGACAGATG ATCTGCCGCC TTCGGAGAAT GATCTAAAGC GCAAGAGGGA AAGCTTCGAC 
AATGTGTCCC TGATGTCAGT GGACTCCTTT GCCCTGCCGG CGAGCACGAA AAAACCCAAA 
CTCATACGCA CAGGATCAAT TTCGAGGACG CTTCGGCGAT CTGTTAGCTT CGTTGCCGAA 
CCATTGATGA AAACTTTACG ACCACGCAGA AGTTCAGTGG CGGATGCATC CAGTTTCGTT 
GGTAGCGAGG CAGCCGATAA TTCAATTTGC TCACTAGCCT CTTCCATAAA TTTAACCCTA 
AACGACTCCA TTAGAAAGCC AGTTAAGGAG AAGCTGCGCA GCTTTTGCGG CAGGATTACA 
AGAAGCAGCA GTCGTAGGCA TGACAGAGGT GTCGACGGCA CTCCGGAATG TACGACCCTA 
TTGGATAGTG GCTTCAAGAC ACCAAAAGCA CCCAAGCTCA GATCTACGGC CACGCCCAAA 
ACAGCCAAGC GACTCTTCGG CCCCGTCTCC GGTGTTGTTT CCGCATTGAC CCTAACTAAC 
CCATCCACAC CCACACCGAA TCCTAATCCG AATACGAATT GCACCGCCAC TTTGGATGTC 
AACGATACCT CCATACACAC ATTCGCTTTC TGTGCTGCTG CCGTCACGCC TGCAATGCCA 
CCATCATCAT CATCATCATC GTCATCTCCC ATACTATCGA CTTCGATCGC TGTACATAGC 
TCGGTGCAAG TAATTAAGTC TCCCGAAGAG TCGACAACGA GGCGTAACAG CCCCGCGCCC 
ATAGAAGAGC CCATGGCAAT GGTGGTTGAC GAGCACACGA CTTTGACGAA GCCGGAGCCG 
AAGAATAATC ACACTCATAT CCTGAAATCA GACTGGTTCT GGTATACCAT ACAGAATGGC 
TATGCCAATG AGATGGACTA TCTGTTTGGC GACTATTTGG ATAGCATCAC GAATACACCG 
AACACAGATC GTCGGGATTC ATTGCCCATT AGCTTTAACA AAAGGAAACG CAAGCGTTTT 
TCGCAACGCA TCCAGCTGGA GGGTACTCCG TTGGGTTCTG GTAAACGACG CTCCTCCGTT 
TCGGATGCCG GTCTATTGTC TGTCTCAAAT AGTTTATTCG ATTGTACCAC CAGTCCGGAT 
AAACTGGAGT CGGACAAGCT GTTGCATGCC GAGCCGGAGG CGAGCGATGC CACTCCAGCC 
AAGAAATCGA TGCGCTTTAA TCACTTTATG GACTTTTATA CCACAGAGTC CAACTACGTG 
GGCATATTGG ACACCATACT GAATCTTTTT AAAAACAAGT TGGAAGAGTT GGCTGAAACC 
AATGATCCGC TGCTTAATAA GTCGGAGATC AAGACGATCT TTGGCAACTT CCTGCCTATC 
CACGAGGTAC ACCAAAGCAT GCTGGAGCAT CTGCGGAAAT TGCATGCTAA CTGGCGGGAG 
GATTGTCTTA TTGGAGACAT TATCATTCAA CATCGCGACG AGCTGATAAA GGCGTATCCA 
CCATACGTCA ACTTCTTCGA GCAGATGAAG GAGCAACTGC AGTACTGCGA TCGGGAATAT 
CCGCGTTTCC ACGCCTTTCT GAAGATTAAT CAAACGAAAC CGGAGTGCGG ACGCCAAGGT 
CTACAAGATC TGATGATCCG TCCAGTTCAG CGATTGCCCA GTATCAGTCT GCTGCTAAAT 
GATATATTGA AACATACGAC AAGTGGCAAT GCGGACCACG GACGTTTGGA GGAGGCTCTG 
AAGGCCATCA AGCAGGTGAC ACTGCACATC AACGAGGATA AAAGGCGGAC CGAATCGCGC 
ATGGCCATCT TCGATATATT CAACGATATC GAAGGATGTC CGGCGCATTT AGTGAGTTCC 
AACCGCAGCT TTATTTTGAA GTGTGAGGTG AACGAGCTCT CCGATTCGCT GAGCGGTCGT 
GGTGATAGCC TGGTCCTGTA CCTGTTCTCC GATTCCATTG AGCTATGCAA GCGGCGTTCT 
AAAGGATTCA ACACTGCAAA ATCGCCAAGC ACGGCCAAGA CGCATAAGCA TCTCAAGCTG 
ATATCTTTGA ATACGATCCG GTTAGTGATT GACATCTCGG ATAGTCCAAG AGCTTTTGGT 
TTGCTCTTGC GGGGCGATAA GGAGAAGCTC TATACGTTTA CGATCAGTGA TGAGGAGACA 
GATAAGTCGG TCTACGTAAA GAAATTGTGC AACCAGATTG CAGCCCACAC CTGCCGTACT 
GATGCGGACA AACTTTTGAT TTGTCGCACC TCCCAGGAAC TGGAGGTGGA CATAAGTGAT 
GTGAATGTCA GCACGCTCAG CAAAGCCTTT AAACTGGCGG CCAAAACGCG ACTCAAGGTG 
GGTCGTGCTT TCTCCTTTAA CAAAACGCCC AACAAACTGA AACGGGCCGT ATCCACGATG 
ATGACTTCGC CGTTTGGCAG CACCAACTCT CTAACACCTG CCTCTCAATT GGCCCAGATG 
CGTTTGGCCA GCTGCACGAA TATAAATGAG GTTGATGATG AGGACTGTGC AAGCATGCGA 
AGCAGTTCGC CGTCAACACA ATCCGAGATG CTAGTGGTAC CGCCACTTTC GGTGCAACCC 
ACGCGGAAAA ACAAGGCCGT TGTGGGCCGC ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523965.2
CDS	497..4432
Translation	MEMETIEEQSKCEMSITTLPTRICLVGGVGQDADTLQAAESFGLPIVTSDTGLDILGESSDWRTFYVLDDFEGASFEAIHKQKECILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRKKDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWADRNSLEFDATQENFTKTHRLKAFEGQKICFFGFPVEEHQHMVDVLLENGGVCAELDDPECSHVVMPNTGAVFTSTSTSTDTNPSTLINPPESQVPGSSSASQAGEEEQRKAEHLEQAEIKKMEYSHAETDNGMSLDIDQEQKENQGIEDEDDIHFDERLFVEAADIINHGEDQLPESDEEDLEEEQKPKVEEVLKEQEVEEDVQASTPKTSKIRTSLKAATPLSSPEMDGIALDFESTTQNISPILKAIDECDLETDDLPPSENDLKRKRESFDNVSLMSVDSFALPASTKKPKLIRTGSISRTLRRSVSFVAEPLMKTLRPRRSSVADASSFVGSEAADNSICSLASSINLTLNDSIRKPVKEKLRSFCGRITRSSSRRHDRGVDGTPECTTLLDSGFKTPKAPKLRSTATPKTAKRLFGPVSGVVSALTLTNPSTPTPNPNPNTNCTATLDVNDTSIHTFAFCAAAVTPAMPPSSSSSSSSPILSTSIAVHSSVQVIKSPEESTTRRNSPAPIEEPMAMVVDEHTTLTKPEPKNNHTHILKSDWFWYTIQNGYANEMDYLFGDYLDSITNTPNTDRRDSLPISFNKRKRKRFSQRIQLEGTPLGSGKRRSSVSDAGLLSVSNSLFDCTTSPDKLESDKLLHAEPEASDATPAKKSMRFNHFMDFYTTESNYVGILDTILNLFKNKLEELAETNDPLLNKSEIKTIFGNFLPIHEVHQSMLEHLRKLHANWREDCLIGDIIIQHRDELIKAYPPYVNFFEQMKEQLQYCDREYPRFHAFLKINQTKPECGRQGLQDLMIRPVQRLPSISLLLNDILKHTTSGNADHGRLEEALKAIKQVTLHINEDKRRTESRMAIFDIFNDIEGCPAHLVSSNRSFILKCEVNELSDSLSGRGDSLVLYLFSDSIELCKRRSKGFNTAKSPSTAKTHKHLKLISLNTIRLVIDISDSPRAFGLLLRGDKEKLYTFTISDEETDKSVYVKKLCNQIAAHTCRTDADKLLICRTSQELEVDISDVNVSTLSKAFKLAAKTRLKVGRAFSFNKTPNKLKRAVSTMMTSPFGSTNSLTPASQLAQMRLASCTNINEVDDEDCASMRSSSPSTQSEMLVVPPLSVQPTRKNKAVVGRI

Target ORF information:

RefSeq Version	NM_079241.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pebble (pbl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079241.4

ORF Insert Sequence:

ATGGAAATGG AGACCATTGA AGAGCAATCG AAGTGCGAGA TGTCCATAAC AACGCTGCCC 
ACGCGCATCT GCCTGGTGGG AGGCGTTGGC CAGGATGCGG ACACGCTGCA GGCAGCCGAA 
TCCTTTGGCC TGCCCATCGT TACGTCGGAC ACGGGTCTGG ACATTCTCGG AGAGTCGTCG 
GACTGGCGGA CATTCTACGT GCTGGATGAC TTCGAGGGTG CCAGCTTCGA GGCGATACAC 
AAGCAAAAGG AATGCATTCT GGGACCGCCG GCTTTGAAGT ACGCGGCCGA GATGAAACAG 
ACACTGGGCC AGAACTCGCG GCCCATTTAC AATTACGCGA TGCGCGGCGT GGTCACTTGT 
TTTACAGGCA TACGCAAGAA GGATGAGCTG ACAAAGCTGG TCAATCTCAT TCACTCGATG 
GGTGGCTGCA TCAAGAAGGA CCTGAACACG AAGACCACGC ATTTGATTTG CAACCACAGT 
GGCGGCGAAA AGTACCAGTA CGCCAAAACC TTTCGGTTGA CCGTGGTTCG TCCTGCTTGG 
GTGTTTGCCG CTTGGGCGGA TCGTAATTCG CTGGAGTTCG ATGCCACGCA AGAGAATTTC 
ACCAAGACGC ATCGCCTGAA GGCATTCGAG GGTCAAAAGA TTTGCTTCTT TGGATTCCCT 
GTGGAGGAGC ATCAGCATAT GGTGGATGTA CTTTTAGAAA ACGGAGGCGT TTGCGCCGAG 
CTCGATGATC CGGAGTGCTC GCATGTCGTG ATGCCCAATA CGGGCGCAGT ATTTACAAGC 
ACTAGCACTA GCACAGATAC TAACCCAAGC ACCCTAATTA ACCCACCCGA ATCCCAAGTC 
CCAGGCAGTA GTAGTGCATC GCAGGCGGGG GAAGAAGAGC AACGAAAGGC TGAGCATCTT 
GAGCAGGCGG AAATCAAAAA GATGGAATAC TCACACGCGG AAACGGACAA TGGAATGAGT 
TTGGACATTG ACCAGGAGCA AAAAGAGAAT CAAGGCATTG AAGACGAAGA TGACATACAC 
TTTGACGAAC GCCTGTTTGT GGAGGCGGCC GATATTATAA ACCACGGAGA AGACCAATTG 
CCTGAATCTG ATGAAGAAGA CCTGGAGGAG GAGCAGAAAC CCAAGGTCGA GGAGGTTCTA 
AAAGAACAGG AAGTTGAGGA GGATGTGCAA GCCAGTACGC CAAAGACTAG TAAAATAAGA 
ACTAGCTTAA AGGCTGCCAC GCCTCTTAGC TCACCCGAAA TGGATGGCAT TGCTTTGGAC 
TTCGAGAGCA CCACCCAGAA CATTTCACCC ATTTTAAAAG CTATTGACGA GTGCGACTTA 
GAGACAGATG ATCTGCCGCC TTCGGAGAAT GATCTAAAGC GCAAGAGGGA AAGCTTCGAC 
AATGTGTCCC TGATGTCAGT GGACTCCTTT GCCCTGCCGG CGAGCACGAA AAAACCCAAA 
CTCATACGCA CAGGATCAAT TTCGAGGACG CTTCGGCGAT CTGTTAGCTT CGTTGCCGAA 
CCATTGATGA AAACTTTACG ACCACGCAGA AGTTCAGTGG CGGATGCATC CAGTTTCGTT 
GGTAGCGAGG CAGCCGATAA TTCAATTTGC TCACTAGCCT CTTCCATAAA TTTAACCCTA 
AACGACTCCA TTAGAAAGCC AGTTAAGGAG AAGCTGCGCA GCTTTTGCGG CAGGATTACA 
AGAAGCAGCA GTCGTAGGCA TGACAGAGGT GTCGACGGCA CTCCGGAATG TACGACCCTA 
TTGGATAGTG GCTTCAAGAC ACCAAAAGCA CCCAAGCTCA GATCTACGGC CACGCCCAAA 
ACAGCCAAGC GACTCTTCGG CCCCGTCTCC GGTGTTGTTT CCGCATTGAC CCTAACTAAC 
CCATCCACAC CCACACCGAA TCCTAATCCG AATACGAATT GCACCGCCAC TTTGGATGTC 
AACGATACCT CCATACACAC ATTCGCTTTC TGTGCTGCTG CCGTCACGCC TGCAATGCCA 
CCATCATCAT CATCATCATC GTCATCTCCC ATACTATCGA CTTCGATCGC TGTACATAGC 
TCGGTGCAAG TAATTAAGTC TCCCGAAGAG TCGACAACGA GGCGTAACAG CCCCGCGCCC 
ATAGAAGAGC CCATGGCAAT GGTGGTTGAC GAGCACACGA CTTTGACGAA GCCGGAGCCG 
AAGAATAATC ACACTCATAT CCTGAAATCA GACTGGTTCT GGTATACCAT ACAGAATGGC 
TATGCCAATG AGATGGACTA TCTGTTTGGC GACTATTTGG ATAGCATCAC GAATACACCG 
AACACAGATC GTCGGGATTC ATTGCCCATT AGCTTTAACA AAAGGAAACG CAAGCGTTTT 
TCGCAACGCA TCCAGCTGGA GGGTACTCCG TTGGGTTCTG GTAAACGACG CTCCTCCGTT 
TCGGATGCCG GTCTATTGTC TGTCTCAAAT AGTTTATTCG ATTGTACCAC CAGTCCGGAT 
AAACTGGAGT CGGACAAGCT GTTGCATGCC GAGCCGGAGG CGAGCGATGC CACTCCAGCC 
AAGAAATCGA TGCGCTTTAA TCACTTTATG GACTTTTATA CCACAGAGTC CAACTACGTG 
GGCATATTGG ACACCATACT GAATCTTTTT AAAAACAAGT TGGAAGAGTT GGCTGAAACC 
AATGATCCGC TGCTTAATAA GTCGGAGATC AAGACGATCT TTGGCAACTT CCTGCCTATC 
CACGAGGTAC ACCAAAGCAT GCTGGAGCAT CTGCGGAAAT TGCATGCTAA CTGGCGGGAG 
GATTGTCTTA TTGGAGACAT TATCATTCAA CATCGCGACG AGCTGATAAA GGCGTATCCA 
CCATACGTCA ACTTCTTCGA GCAGATGAAG GAGCAACTGC AGTACTGCGA TCGGGAATAT 
CCGCGTTTCC ACGCCTTTCT GAAGATTAAT CAAACGAAAC CGGAGTGCGG ACGCCAAGGT 
CTACAAGATC TGATGATCCG TCCAGTTCAG CGATTGCCCA GTATCAGTCT GCTGCTAAAT 
GATATATTGA AACATACGAC AAGTGGCAAT GCGGACCACG GACGTTTGGA GGAGGCTCTG 
AAGGCCATCA AGCAGGTGAC ACTGCACATC AACGAGGATA AAAGGCGGAC CGAATCGCGC 
ATGGCCATCT TCGATATATT CAACGATATC GAAGGATGTC CGGCGCATTT AGTGAGTTCC 
AACCGCAGCT TTATTTTGAA GTGTGAGGTG AACGAGCTCT CCGATTCGCT GAGCGGTCGT 
GGTGATAGCC TGGTCCTGTA CCTGTTCTCC GATTCCATTG AGCTATGCAA GCGGCGTTCT 
AAAGGATTCA ACACTGCAAA ATCGCCAAGC ACGGCCAAGA CGCATAAGCA TCTCAAGCTG 
ATATCTTTGA ATACGATCCG GTTAGTGATT GACATCTCGG ATAGTCCAAG AGCTTTTGGT 
TTGCTCTTGC GGGGCGATAA GGAGAAGCTC TATACGTTTA CGATCAGTGA TGAGGAGACA 
GATAAGTCGG TCTACGTAAA GAAATTGTGC AACCAGATTG CAGCCCACAC CTGCCGTACT 
GATGCGGACA AACTTTTGAT TTGTCGCACC TCCCAGGAAC TGGAGGTGGA CATAAGTGAT 
GTGAATGTCA GCACGCTCAG CAAAGCCTTT AAACTGGCGG CCAAAACGCG ACTCAAGGTG 
GGTCGTGCTT TCTCCTTTAA CAAAACGCCC AACAAACTGA AACGGGCCGT ATCCACGATG 
ATGACTTCGC CGTTTGGCAG CACCAACTCT CTAACACCTG CCTCTCAATT GGCCCAGATG 
CGTTTGGCCA GCTGCACGAA TATAAATGAG GTTGATGATG AGGACTGTGC AAGCATGCGA 
AGCAGTTCGC CGTCAACACA ATCCGAGATG CTAGTGGTAC CGCCACTTTC GGTGCAACCC 
ACGCGGAAAA ACAAGGCCGT TGTGGGCCGC ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pbl ( NM_168243.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729308.1 Drosophila melanogaster pebble (pbl), transcript variant D, mRNA.

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CloneID	OFa17844
Clone ID Related Accession (Same CDS sequence)	NM_168243.3
Accession Version	NM_168243.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pebble, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_168243.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAG GGGAGAAAAC GGCAAAAGGA AGATGGAAAC TCATTCTGGG ACCGCCGGCT 
TTGAAGTACG CGGCCGAGAT GAAACAGACA CTGGGCCAGA ACTCGCGGCC CATTTACAAT 
TACGCGATGC GCGGCGTGGT CACTTGTTTT ACAGGCATAC GCAAGAAGGA TGAGCTGACA 
AAGCTGGTCA ATCTCATTCA CTCGATGGGT GGCTGCATCA AGAAGGACCT GAACACGAAG 
ACCACGCATT TGATTTGCAA CCACAGTGGC GGCGAAAAGT ACCAGTACGC CAAAACCTTT 
CGGTTGACCG TGGTTCGTCC TGCTTGGGTG TTTGCCGCTT GGGCGGATCG TAATTCGCTG 
GAGTTCGATG CCACGCAAGA GAATTTCACC AAGACGCATC GCCTGAAGGC ATTCGAGGGT 
CAAAAGATTT GCTTCTTTGG ATTCCCTGTG GAGGAGCATC AGCATATGGT GGATGTACTT 
TTAGAAAACG GAGGCGTTTG CGCCGAGCTC GATGATCCGG AGTGCTCGCA TGTCGTGATG 
CCCAATACGG GCGCAGTATT TACAAGCACT AGCACTAGCA CAGATACTAA CCCAAGCACC 
CTAATTAACC CACCCGAATC CCAAGTCCCA GGCAGTAGTA GTGCATCGCA GGCGGGGGAA 
GAAGAGCAAC GAAAGGCTGA GCATCTTGAG CAGGCGGAAA TCAAAAAGAT GGAATACTCA 
CACGCGGAAA CGGACAATGG AATGAGTTTG GACATTGACC AGGAGCAAAA AGAGAATCAA 
GGCATTGAAG ACGAAGATGA CATACACTTT GACGAACGCC TGTTTGTGGA GGCGGCCGAT 
ATTATAAACC ACGGAGAAGA CCAATTGCCT GAATCTGATG AAGAAGACCT GGAGGAGGAG 
CAGAAACCCA AGGTCGAGGA GGTTCTAAAA GAACAGGAAG TTGAGGAGGA TGTGCAAGCC 
AGTACGCCAA AGACTAGTAA AATAAGAACT AGCTTAAAGG CTGCCACGCC TCTTAGCTCA 
CCCGAAATGG ATGGCATTGC TTTGGACTTC GAGAGCACCA CCCAGAACAT TTCACCCATT 
TTAAAAGCTA TTGACGAGTG CGACTTAGAG ACAGATGATC TGCCGCCTTC GGAGAATGAT 
CTAAAGCGCA AGAGGGAAAG CTTCGACAAT GTGTCCCTGA TGTCAGTGGA CTCCTTTGCC 
CTGCCGGCGA GCACGAAAAA ACCCAAACTC ATACGCACAG GATCAATTTC GAGGACGCTT 
CGGCGATCTG TTAGCTTCGT TGCCGAACCA TTGATGAAAA CTTTACGACC ACGCAGAAGT 
TCAGTGGCGG ATGCATCCAG TTTCGTTGGT AGCGAGGCAG CCGATAATTC AATTTGCTCA 
CTAGCCTCTT CCATAAATTT AACCCTAAAC GACTCCATTA GAAAGCCAGT TAAGGAGAAG 
CTGCGCAGCT TTTGCGGCAG GATTACAAGA AGCAGCAGTC GTAGGCATGA CAGAGGTGTC 
GACGGCACTC CGGAATGTAC GACCCTATTG GATAGTGGCT TCAAGACACC AAAAGCACCC 
AAGCTCAGAT CTACGGCCAC GCCCAAAACA GCCAAGCGAC TCTTCGGCCC CGTCTCCGGT 
GTTGTTTCCG CATTGACCCT AACTAACCCA TCCACACCCA CACCGAATCC TAATCCGAAT 
ACGAATTGCA CCGCCACTTT GGATGTCAAC GATACCTCCA TACACACATT CGCTTTCTGT 
GCTGCTGCCG TCACGCCTGC AATGCCACCA TCATCATCAT CATCATCGTC ATCTCCCATA 
CTATCGACTT CGATCGCTGT ACATAGCTCG GTGCAAGTAA TTAAGTCTCC CGAAGAGTCG 
ACAACGAGGC GTAACAGCCC CGCGCCCATA GAAGAGCCCA TGGCAATGGT GGTTGACGAG 
CACACGACTT TGACGAAGCC GGAGCCGAAG AATAATCACA CTCATATCCT GAAATCAGAC 
TGGTTCTGGT ATACCATACA GAATGGCTAT GCCAATGAGA TGGACTATCT GTTTGGCGAC 
TATTTGGATA GCATCACGAA TACACCGAAC ACAGATCGTC GGGATTCATT GCCCATTAGC 
TTTAACAAAA GGAAACGCAA GCGTTTTTCG CAACGCATCC AGCTGGAGGG TACTCCGTTG 
GGTTCTGGTA AACGACGCTC CTCCGTTTCG GATGCCGGTC TATTGTCTGT CTCAAATAGT 
TTATTCGATT GTACCACCAG TCCGGATAAA CTGGAGTCGG ACAAGCTGTT GCATGCCGAG 
CCGGAGGCGA GCGATGCCAC TCCAGCCAAG AAATCGATGC GCTTTAATCA CTTTATGGAC 
TTTTATACCA CAGAGTCCAA CTACGTGGGC ATATTGGACA CCATACTGAA TCTTTTTAAA 
AACAAGTTGG AAGAGTTGGC TGAAACCAAT GATCCGCTGC TTAATAAGTC GGAGATCAAG 
ACGATCTTTG GCAACTTCCT GCCTATCCAC GAGGTACACC AAAGCATGCT GGAGCATCTG 
CGGAAATTGC ATGCTAACTG GCGGGAGGAT TGTCTTATTG GAGACATTAT CATTCAACAT 
CGCGACGAGC TGATAAAGGC GTATCCACCA TACGTCAACT TCTTCGAGCA GATGAAGGAG 
CAACTGCAGT ACTGCGATCG GGAATATCCG CGTTTCCACG CCTTTCTGAA GATTAATCAA 
ACGAAACCGG AGTGCGGACG CCAAGGTCTA CAAGATCTGA TGATCCGTCC AGTTCAGCGA 
TTGCCCAGTA TCAGTCTGCT GCTAAATGAT ATATTGAAAC ATACGACAAG TGGCAATGCG 
GACCACGGAC GTTTGGAGGA GGCTCTGAAG GCCATCAAGC AGGTGACACT GCACATCAAC 
GAGGATAAAA GGCGGACCGA ATCGCGCATG GCCATCTTCG ATATATTCAA CGATATCGAA 
GGATGTCCGG CGCATTTAGT GAGTTCCAAC CGCAGCTTTA TTTTGAAGTG TGAGGTGAAC 
GAGCTCTCCG ATTCGCTGAG CGGTCGTGGT GATAGCCTGG TCCTGTACCT GTTCTCCGAT 
TCCATTGAGC TATGCAAGCG GCGTTCTAAA GGATTCAACA CTGCAAAATC GCCAAGCACG 
GCCAAGACGC ATAAGCATCT CAAGCTGATA TCTTTGAATA CGATCCGGTT AGTGATTGAC 
ATCTCGGATA GTCCAAGAGC TTTTGGTTTG CTCTTGCGGG GCGATAAGGA GAAGCTCTAT 
ACGTTTACGA TCAGTGATGA GGAGACAGAT AAGTCGGTCT ACGTAAAGAA ATTGTGCAAC 
CAGATTGCAG CCCACACCTG CCGTACTGAT GCGGACAAAC TTTTGATTTG TCGCACCTCC 
CAGGAACTGG AGGTGGACAT AAGTGATGTG AATGTCAGCA CGCTCAGCAA AGCCTTTAAA 
CTGGCGGCCA AAACGCGACT CAAGGTGGGT CGTGCTTTCT CCTTTAACAA AACGCCCAAC 
AAACTGAAAC GGGCCGTATC CACGATGATG ACTTCGCCGT TTGGCAGCAC CAACTCTCTA 
ACACCTGCCT CTCAATTGGC CCAGATGCGT TTGGCCAGCT GCACGAATAT AAATGAGGTT 
GATGATGAGG ACTGTGCAAG CATGCGAAGC AGTTCGCCGT CAACACAATC CGAGATGCTA 
GTGGTACCGC CACTTTCGGT GCAACCCACG CGGAAAAACA AGGCCGTTGT GGGCCGCATT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729308.1
CDS	348..4070
Translation	MESGEKTAKGRWKLILGPPALKYAAEMKQTLGQNSRPIYNYAMRGVVTCFTGIRKKDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVFAAWADRNSLEFDATQENFTKTHRLKAFEGQKICFFGFPVEEHQHMVDVLLENGGVCAELDDPECSHVVMPNTGAVFTSTSTSTDTNPSTLINPPESQVPGSSSASQAGEEEQRKAEHLEQAEIKKMEYSHAETDNGMSLDIDQEQKENQGIEDEDDIHFDERLFVEAADIINHGEDQLPESDEEDLEEEQKPKVEEVLKEQEVEEDVQASTPKTSKIRTSLKAATPLSSPEMDGIALDFESTTQNISPILKAIDECDLETDDLPPSENDLKRKRESFDNVSLMSVDSFALPASTKKPKLIRTGSISRTLRRSVSFVAEPLMKTLRPRRSSVADASSFVGSEAADNSICSLASSINLTLNDSIRKPVKEKLRSFCGRITRSSSRRHDRGVDGTPECTTLLDSGFKTPKAPKLRSTATPKTAKRLFGPVSGVVSALTLTNPSTPTPNPNPNTNCTATLDVNDTSIHTFAFCAAAVTPAMPPSSSSSSSSPILSTSIAVHSSVQVIKSPEESTTRRNSPAPIEEPMAMVVDEHTTLTKPEPKNNHTHILKSDWFWYTIQNGYANEMDYLFGDYLDSITNTPNTDRRDSLPISFNKRKRKRFSQRIQLEGTPLGSGKRRSSVSDAGLLSVSNSLFDCTTSPDKLESDKLLHAEPEASDATPAKKSMRFNHFMDFYTTESNYVGILDTILNLFKNKLEELAETNDPLLNKSEIKTIFGNFLPIHEVHQSMLEHLRKLHANWREDCLIGDIIIQHRDELIKAYPPYVNFFEQMKEQLQYCDREYPRFHAFLKINQTKPECGRQGLQDLMIRPVQRLPSISLLLNDILKHTTSGNADHGRLEEALKAIKQVTLHINEDKRRTESRMAIFDIFNDIEGCPAHLVSSNRSFILKCEVNELSDSLSGRGDSLVLYLFSDSIELCKRRSKGFNTAKSPSTAKTHKHLKLISLNTIRLVIDISDSPRAFGLLLRGDKEKLYTFTISDEETDKSVYVKKLCNQIAAHTCRTDADKLLICRTSQELEVDISDVNVSTLSKAFKLAAKTRLKVGRAFSFNKTPNKLKRAVSTMMTSPFGSTNSLTPASQLAQMRLASCTNINEVDDEDCASMRSSSPSTQSEMLVVPPLSVQPTRKNKAVVGRI

Target ORF information:

RefSeq Version	NM_168243.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pebble (pbl), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168243.3

ORF Insert Sequence:

ATGGAGTCAG GGGAGAAAAC GGCAAAAGGA AGATGGAAAC TCATTCTGGG ACCGCCGGCT 
TTGAAGTACG CGGCCGAGAT GAAACAGACA CTGGGCCAGA ACTCGCGGCC CATTTACAAT 
TACGCGATGC GCGGCGTGGT CACTTGTTTT ACAGGCATAC GCAAGAAGGA TGAGCTGACA 
AAGCTGGTCA ATCTCATTCA CTCGATGGGT GGCTGCATCA AGAAGGACCT GAACACGAAG 
ACCACGCATT TGATTTGCAA CCACAGTGGC GGCGAAAAGT ACCAGTACGC CAAAACCTTT 
CGGTTGACCG TGGTTCGTCC TGCTTGGGTG TTTGCCGCTT GGGCGGATCG TAATTCGCTG 
GAGTTCGATG CCACGCAAGA GAATTTCACC AAGACGCATC GCCTGAAGGC ATTCGAGGGT 
CAAAAGATTT GCTTCTTTGG ATTCCCTGTG GAGGAGCATC AGCATATGGT GGATGTACTT 
TTAGAAAACG GAGGCGTTTG CGCCGAGCTC GATGATCCGG AGTGCTCGCA TGTCGTGATG 
CCCAATACGG GCGCAGTATT TACAAGCACT AGCACTAGCA CAGATACTAA CCCAAGCACC 
CTAATTAACC CACCCGAATC CCAAGTCCCA GGCAGTAGTA GTGCATCGCA GGCGGGGGAA 
GAAGAGCAAC GAAAGGCTGA GCATCTTGAG CAGGCGGAAA TCAAAAAGAT GGAATACTCA 
CACGCGGAAA CGGACAATGG AATGAGTTTG GACATTGACC AGGAGCAAAA AGAGAATCAA 
GGCATTGAAG ACGAAGATGA CATACACTTT GACGAACGCC TGTTTGTGGA GGCGGCCGAT 
ATTATAAACC ACGGAGAAGA CCAATTGCCT GAATCTGATG AAGAAGACCT GGAGGAGGAG 
CAGAAACCCA AGGTCGAGGA GGTTCTAAAA GAACAGGAAG TTGAGGAGGA TGTGCAAGCC 
AGTACGCCAA AGACTAGTAA AATAAGAACT AGCTTAAAGG CTGCCACGCC TCTTAGCTCA 
CCCGAAATGG ATGGCATTGC TTTGGACTTC GAGAGCACCA CCCAGAACAT TTCACCCATT 
TTAAAAGCTA TTGACGAGTG CGACTTAGAG ACAGATGATC TGCCGCCTTC GGAGAATGAT 
CTAAAGCGCA AGAGGGAAAG CTTCGACAAT GTGTCCCTGA TGTCAGTGGA CTCCTTTGCC 
CTGCCGGCGA GCACGAAAAA ACCCAAACTC ATACGCACAG GATCAATTTC GAGGACGCTT 
CGGCGATCTG TTAGCTTCGT TGCCGAACCA TTGATGAAAA CTTTACGACC ACGCAGAAGT 
TCAGTGGCGG ATGCATCCAG TTTCGTTGGT AGCGAGGCAG CCGATAATTC AATTTGCTCA 
CTAGCCTCTT CCATAAATTT AACCCTAAAC GACTCCATTA GAAAGCCAGT TAAGGAGAAG 
CTGCGCAGCT TTTGCGGCAG GATTACAAGA AGCAGCAGTC GTAGGCATGA CAGAGGTGTC 
GACGGCACTC CGGAATGTAC GACCCTATTG GATAGTGGCT TCAAGACACC AAAAGCACCC 
AAGCTCAGAT CTACGGCCAC GCCCAAAACA GCCAAGCGAC TCTTCGGCCC CGTCTCCGGT 
GTTGTTTCCG CATTGACCCT AACTAACCCA TCCACACCCA CACCGAATCC TAATCCGAAT 
ACGAATTGCA CCGCCACTTT GGATGTCAAC GATACCTCCA TACACACATT CGCTTTCTGT 
GCTGCTGCCG TCACGCCTGC AATGCCACCA TCATCATCAT CATCATCGTC ATCTCCCATA 
CTATCGACTT CGATCGCTGT ACATAGCTCG GTGCAAGTAA TTAAGTCTCC CGAAGAGTCG 
ACAACGAGGC GTAACAGCCC CGCGCCCATA GAAGAGCCCA TGGCAATGGT GGTTGACGAG 
CACACGACTT TGACGAAGCC GGAGCCGAAG AATAATCACA CTCATATCCT GAAATCAGAC 
TGGTTCTGGT ATACCATACA GAATGGCTAT GCCAATGAGA TGGACTATCT GTTTGGCGAC 
TATTTGGATA GCATCACGAA TACACCGAAC ACAGATCGTC GGGATTCATT GCCCATTAGC 
TTTAACAAAA GGAAACGCAA GCGTTTTTCG CAACGCATCC AGCTGGAGGG TACTCCGTTG 
GGTTCTGGTA AACGACGCTC CTCCGTTTCG GATGCCGGTC TATTGTCTGT CTCAAATAGT 
TTATTCGATT GTACCACCAG TCCGGATAAA CTGGAGTCGG ACAAGCTGTT GCATGCCGAG 
CCGGAGGCGA GCGATGCCAC TCCAGCCAAG AAATCGATGC GCTTTAATCA CTTTATGGAC 
TTTTATACCA CAGAGTCCAA CTACGTGGGC ATATTGGACA CCATACTGAA TCTTTTTAAA 
AACAAGTTGG AAGAGTTGGC TGAAACCAAT GATCCGCTGC TTAATAAGTC GGAGATCAAG 
ACGATCTTTG GCAACTTCCT GCCTATCCAC GAGGTACACC AAAGCATGCT GGAGCATCTG 
CGGAAATTGC ATGCTAACTG GCGGGAGGAT TGTCTTATTG GAGACATTAT CATTCAACAT 
CGCGACGAGC TGATAAAGGC GTATCCACCA TACGTCAACT TCTTCGAGCA GATGAAGGAG 
CAACTGCAGT ACTGCGATCG GGAATATCCG CGTTTCCACG CCTTTCTGAA GATTAATCAA 
ACGAAACCGG AGTGCGGACG CCAAGGTCTA CAAGATCTGA TGATCCGTCC AGTTCAGCGA 
TTGCCCAGTA TCAGTCTGCT GCTAAATGAT ATATTGAAAC ATACGACAAG TGGCAATGCG 
GACCACGGAC GTTTGGAGGA GGCTCTGAAG GCCATCAAGC AGGTGACACT GCACATCAAC 
GAGGATAAAA GGCGGACCGA ATCGCGCATG GCCATCTTCG ATATATTCAA CGATATCGAA 
GGATGTCCGG CGCATTTAGT GAGTTCCAAC CGCAGCTTTA TTTTGAAGTG TGAGGTGAAC 
GAGCTCTCCG ATTCGCTGAG CGGTCGTGGT GATAGCCTGG TCCTGTACCT GTTCTCCGAT 
TCCATTGAGC TATGCAAGCG GCGTTCTAAA GGATTCAACA CTGCAAAATC GCCAAGCACG 
GCCAAGACGC ATAAGCATCT CAAGCTGATA TCTTTGAATA CGATCCGGTT AGTGATTGAC 
ATCTCGGATA GTCCAAGAGC TTTTGGTTTG CTCTTGCGGG GCGATAAGGA GAAGCTCTAT 
ACGTTTACGA TCAGTGATGA GGAGACAGAT AAGTCGGTCT ACGTAAAGAA ATTGTGCAAC 
CAGATTGCAG CCCACACCTG CCGTACTGAT GCGGACAAAC TTTTGATTTG TCGCACCTCC 
CAGGAACTGG AGGTGGACAT AAGTGATGTG AATGTCAGCA CGCTCAGCAA AGCCTTTAAA 
CTGGCGGCCA AAACGCGACT CAAGGTGGGT CGTGCTTTCT CCTTTAACAA AACGCCCAAC 
AAACTGAAAC GGGCCGTATC CACGATGATG ACTTCGCCGT TTGGCAGCAC CAACTCTCTA 
ACACCTGCCT CTCAATTGGC CCAGATGCGT TTGGCCAGCT GCACGAATAT AAATGAGGTT 
GATGATGAGG ACTGTGCAAG CATGCGAAGC AGTTCGCCGT CAACACAATC CGAGATGCTA 
GTGGTACCGC CACTTTCGGT GCAACCCACG CGGAAAAACA AGGCCGTTGT GGGCCGCATT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pbl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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