ect2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ect2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ect2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa02114 NM_001113146.1
Latest version!
Xenopus tropicalis epithelial cell transforming 2 (ect2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa31068 XM_012962666.2
Latest version!
Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa31068 XM_012962663.3
Latest version!
Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa31068 XM_012962664.3
Latest version!
Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa31068 XM_031901485.1
Latest version!
Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa31068 XM_012962665.3
Latest version!
Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa31069 XM_012962667.3
Latest version!
Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa31070 XM_012962668.3
Latest version!
Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa31071 XM_012962669.3
Latest version!
Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OXa31069 XM_012962667.2 Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa31068 XM_012962664.2 Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa31070 XM_012962668.2 Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa31068 XM_012962663.2 Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa31068 XM_012962665.2 Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa31071 XM_012962669.2 Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa02114
    Clone ID Related Accession (Same CDS sequence) NM_001113146.1
    Accession Version NM_001113146.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC155458.1. ##Evidence-Data-START## Transcript exon combination :: BC155458.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGGAA ATCAAAGTAC CCTGCATCAA AACAGATTCT
    GTTGAAGAGT TTGTAGAAGC AGACACTCCA GAATTCGAAT CAGTGTTTGT TATATCAGAC
    TTTCAGAAAC CTGCTTATCG ATTTCTTTAC AAAGCTGACT GCAGAGTGCT TGGCCCCCCT
    ATTATAATTT ATTGTGCACA GAAAGGAGAG CCTTTGCCTT TTACATCCCG ACCTCTCTAC
    TGTGCAAGTA TGCTAAATCT CGTATTGTGC TTCACTGGTT TCAGAAAGAA AGAAGAATTG
    GTAAAACTAG TAACTTTGGT TCATCACATG GGAGGTACTA TAAGGAAAGA CTTCAGTTCA
    AAGGTTACCC ATCTTATTGC CAATTCAACT CATGGAGATA AATTTAGGGT TGCAGTTAGT
    CTTGGAACTC CTATAATGAA GGCTGACTGG ATTATCCAAG CTTGGGAGAA AAGGAATGAA
    CTTGAATTCA GTGCAACAAA TGAAGAGTTC AAAAGTAAAT TTAAGGTTCC TCCGTTCCAA
    GACTGCATAT TAAGTTTTCT GGGTTTTTCT GATGAAGACA GAGTCAGCAT GGAAGAAATG
    ACTGAAATGC AAGGGGGAAC ATTTTTATCT GTTGGAGATG AGAAATGCAC TCATCTGGTT
    GTTGAAGAAA ATTCTGTGAA AGAGTTACCG TTTGAACCAC CTAAGAAACT GTACGTGGTA
    AAGCAAGAGT GGTTTTGGGG AAGTATCCAA ATGGATGCCA GAGCAGGAGA AACAATGTAC
    CTGTTTGAAA AGAATGATAG TCCAGCTCTC AAAAAATCTG TCTCTCTGCT CTCCTTGAAT
    ACTCCAAGCA GCAACCGAAA AAAACGTCGC TTAAAAGATA CGCTTGCTCA ATTAACCAGG
    GAGACAGATC TCACACCTTT TCCTCCTCGA AAACGTCCTT CAGCTGAACA TTCCCTTTCT
    ATAGGCTCAC TGCTAGATAT TTCAAATACC CCTGAGTCGG GAAAATCTTT TTCAGATACA
    CCAAAAATTT CAAAGCCTTC AAAAATATCT ACTCCTGTTC CTCAGCAATC AGCAAGGTGG
    CAAGTTGCTA TGGAACTGTA TCAAACTGAG AGTAATTATG TTGACATTCT AACTACAATT
    GTACAGCTTT TTCAACTTCC GTTGGAGAAA GAGGGACAAC TAGGAGGGCC AATTCTTGCT
    CCAGAAGAGA TCAAAACAAT ATTTGGCAGT ATCCCAGATA TTCTTGATGT CCACACTAAT
    ATAAAGGGAG ACCTGGAAAA ACTTATGATT GACTGGGCCG AAAGTAAAAG TATTGGGGAT
    ATTATCCTCA CCTATTCTAA AGATCTGGTG AAAATATATC CACCTTTTGT CAACTTCTTT
    GAAATGAGCA AGGAAATGAT TATAAAATGT GAAAAGCAGA AACCGAGATT TCACGCTTTT
    TTAAAGATCA ACCAGTCAAA GCCAGAATGT GGACGACAAA CTTTAGTCGA GCTCCTTATC
    AGACCTGTTC AACGGTTACC AAGTGTTGCA CTTCTTCTGA ATGATCTCAA AAAGCACACA
    GCAGATGATA ACCCAGACAA AGTAATGTTG GAAAAAGCAA TTGAATCATT AAAAGAAGTC
    ATGATGCACA TTAATGAAGA TAAAAGGAAG ACTGAAGGGC AAAAGCAGAT TTTTGATGTC
    GTCTATGAAG TGGATGGATG TCCTGCAAAT CTGTTATCTT CCCACCGAAT TTTAGTGCAG
    CGTGTAGAAA CAATTGCACT TGGTGAAGAT CTTTGTGATC GAGGAGAACA AGTTACACTT
    TTCCTGTTTA ATGATTGCCT TGAGATTGCA AGAAAACGGC ATAAGGGTAT AGGTGCATTC
    AAGAGTCCTC ATGGTACAAG ACCCCAAGCA TGCCTGAAAC ACATATATTT AATGCCTCTT
    TCACAAATTA AAAAAGTTCT TGACATCAAA GAAACTGAAG AATGTCATAA TGCATTTGCA
    TTGGTAGTGC GACCTCCAAC AGAACAGACA AACCAGTTAT TTAGTTTCCA GCTGACAACA
    GAGGAGCTTC CTAAGGTGGA TTGGCTGAAG ATGCTGTGCC GGCATGTAGC CAACACCATT
    TGTAAAGCAG ATGCTGAAAA TCTGATTTAT TCTACTGACC CTGATTCACT GGAAGTGAGC
    ACAAAGGATG TGGATAGTAC ATTAAGTAGA GCATCAAGGG CCATAAAGAA AACTTCTAAG
    AAAGTTACAA GAGCCTTTTC GTTCACCAAA ACACCCAAGA GAGCTCTTCA AAGGGCCCTG
    ATGGTACAAA ATGCAGATGG CCGAAGCCCT GGCCCAAGTA GTGAGGGTTT TGCAAGCTGC
    CGTATGCCAA GCACCACATC TCTGGCACAT GTTCCTTCTC CGTCCTTGGT AAACCTGTCA
    TCATTGTTTG AGAGGAAGTA CCATACATTT AGCCGATCGA CGACTCACCT TATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106617.1
    CDS158..2794
    Translation

    Target ORF information:

    RefSeq Version NM_001113146.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001113146.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGGAA ATCAAAGTAC CCTGCATCAA AACAGATTCT
    GTTGAAGAGT TTGTAGAAGC AGACACTCCA GAATTCGAAT CAGTGTTTGT TATATCAGAC
    TTTCAGAAAC CTGCTTATCG ATTTCTTTAC AAAGCTGACT GCAGAGTGCT TGGCCCCCCT
    ATTATAATTT ATTGTGCACA GAAAGGAGAG CCTTTGCCTT TTACATCCCG ACCTCTCTAC
    TGTGCAAGTA TGCTAAATCT CGTATTGTGC TTCACTGGTT TCAGAAAGAA AGAAGAATTG
    GTAAAACTAG TAACTTTGGT TCATCACATG GGAGGTACTA TAAGGAAAGA CTTCAGTTCA
    AAGGTTACCC ATCTTATTGC CAATTCAACT CATGGAGATA AATTTAGGGT TGCAGTTAGT
    CTTGGAACTC CTATAATGAA GGCTGACTGG ATTATCCAAG CTTGGGAGAA AAGGAATGAA
    CTTGAATTCA GTGCAACAAA TGAAGAGTTC AAAAGTAAAT TTAAGGTTCC TCCGTTCCAA
    GACTGCATAT TAAGTTTTCT GGGTTTTTCT GATGAAGACA GAGTCAGCAT GGAAGAAATG
    ACTGAAATGC AAGGGGGAAC ATTTTTATCT GTTGGAGATG AGAAATGCAC TCATCTGGTT
    GTTGAAGAAA ATTCTGTGAA AGAGTTACCG TTTGAACCAC CTAAGAAACT GTACGTGGTA
    AAGCAAGAGT GGTTTTGGGG AAGTATCCAA ATGGATGCCA GAGCAGGAGA AACAATGTAC
    CTGTTTGAAA AGAATGATAG TCCAGCTCTC AAAAAATCTG TCTCTCTGCT CTCCTTGAAT
    ACTCCAAGCA GCAACCGAAA AAAACGTCGC TTAAAAGATA CGCTTGCTCA ATTAACCAGG
    GAGACAGATC TCACACCTTT TCCTCCTCGA AAACGTCCTT CAGCTGAACA TTCCCTTTCT
    ATAGGCTCAC TGCTAGATAT TTCAAATACC CCTGAGTCGG GAAAATCTTT TTCAGATACA
    CCAAAAATTT CAAAGCCTTC AAAAATATCT ACTCCTGTTC CTCAGCAATC AGCAAGGTGG
    CAAGTTGCTA TGGAACTGTA TCAAACTGAG AGTAATTATG TTGACATTCT AACTACAATT
    GTACAGCTTT TTCAACTTCC GTTGGAGAAA GAGGGACAAC TAGGAGGGCC AATTCTTGCT
    CCAGAAGAGA TCAAAACAAT ATTTGGCAGT ATCCCAGATA TTCTTGATGT CCACACTAAT
    ATAAAGGGAG ACCTGGAAAA ACTTATGATT GACTGGGCCG AAAGTAAAAG TATTGGGGAT
    ATTATCCTCA CCTATTCTAA AGATCTGGTG AAAATATATC CACCTTTTGT CAACTTCTTT
    GAAATGAGCA AGGAAATGAT TATAAAATGT GAAAAGCAGA AACCGAGATT TCACGCTTTT
    TTAAAGATCA ACCAGTCAAA GCCAGAATGT GGACGACAAA CTTTAGTCGA GCTCCTTATC
    AGACCTGTTC AACGGTTACC AAGTGTTGCA CTTCTTCTGA ATGATCTCAA AAAGCACACA
    GCAGATGATA ACCCAGACAA AGTAATGTTG GAAAAAGCAA TTGAATCATT AAAAGAAGTC
    ATGATGCACA TTAATGAAGA TAAAAGGAAG ACTGAAGGGC AAAAGCAGAT TTTTGATGTC
    GTCTATGAAG TGGATGGATG TCCTGCAAAT CTGTTATCTT CCCACCGAAT TTTAGTGCAG
    CGTGTAGAAA CAATTGCACT TGGTGAAGAT CTTTGTGATC GAGGAGAACA AGTTACACTT
    TTCCTGTTTA ATGATTGCCT TGAGATTGCA AGAAAACGGC ATAAGGGTAT AGGTGCATTC
    AAGAGTCCTC ATGGTACAAG ACCCCAAGCA TGCCTGAAAC ACATATATTT AATGCCTCTT
    TCACAAATTA AAAAAGTTCT TGACATCAAA GAAACTGAAG AATGTCATAA TGCATTTGCA
    TTGGTAGTGC GACCTCCAAC AGAACAGACA AACCAGTTAT TTAGTTTCCA GCTGACAACA
    GAGGAGCTTC CTAAGGTGGA TTGGCTGAAG ATGCTGTGCC GGCATGTAGC CAACACCATT
    TGTAAAGCAG ATGCTGAAAA TCTGATTTAT TCTACTGACC CTGATTCACT GGAAGTGAGC
    ACAAAGGATG TGGATAGTAC ATTAAGTAGA GCATCAAGGG CCATAAAGAA AACTTCTAAG
    AAAGTTACAA GAGCCTTTTC GTTCACCAAA ACACCCAAGA GAGCTCTTCA AAGGGCCCTG
    ATGGTACAAA ATGCAGATGG CCGAAGCCCT GGCCCAAGTA GTGAGGGTTT TGCAAGCTGC
    CGTATGCCAA GCACCACATC TCTGGCACAT GTTCCTTCTC CGTCCTTGGT AAACCTGTCA
    TCATTGTTTG AGAGGAAGTA CCATACATTT AGCCGATCGA CGACTCACCT TATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31068
    Clone ID Related Accession (Same CDS sequence) XM_012962666.2 , XM_012962664.2 , XM_012962665.2 , XM_012962664.3 , XM_031901485.1 , XM_012962663.2 , XM_012962663.3 , XM_012962665.3
    Accession Version XM_012962666.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962666.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818120.1
    CDS72..3029
    Translation

    Target ORF information:

    RefSeq Version XM_012962666.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962666.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31068
    Clone ID Related Accession (Same CDS sequence) XM_012962666.2 , XM_012962664.2 , XM_012962665.2 , XM_012962664.3 , XM_031901485.1 , XM_012962663.2 , XM_012962663.3 , XM_012962665.3
    Accession Version XM_012962664.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818118.1
    CDS227..3184
    Translation

    Target ORF information:

    RefSeq Version XM_012962664.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962664.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31068
    Clone ID Related Accession (Same CDS sequence) XM_012962666.2 , XM_012962664.2 , XM_012962665.2 , XM_012962664.3 , XM_031901485.1 , XM_012962663.2 , XM_012962663.3 , XM_012962665.3
    Accession Version XM_012962665.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962665.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818119.1
    CDS227..3184
    Translation

    Target ORF information:

    RefSeq Version XM_012962665.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962665.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31068
    Clone ID Related Accession (Same CDS sequence) XM_012962666.2 , XM_012962664.2 , XM_012962665.2 , XM_012962664.3 , XM_031901485.1 , XM_012962663.2 , XM_012962663.3 , XM_012962665.3
    Accession Version XM_012962663.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818117.1
    CDS227..3184
    Translation

    Target ORF information:

    RefSeq Version XM_012962663.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962663.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31069
    Clone ID Related Accession (Same CDS sequence) XM_012962667.2 , XM_012962667.3
    Accession Version XM_012962667.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2865bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGGAA ATCAAAGTAC CCTGCATCAA AACAGATTCT
    GTTGAAGAGT TTGTAGAAGC AGACACTCCA GAATTCGAAT CAGTGTTTGT TATATCAGAC
    TTTCAGAAAC CTGCTTATCG ATTTCTTTAC AAAGCTGACT GCAGAGTGCT TGGCCCCCCT
    ATTATAATTT ATTGTGCACA GAAAGGAGAG CCTTTGCCTT TTACATCCCG ACCTCTCTAC
    TGTGCAAGTA TGCTAAATCT CGTATTGTGC TTCACTGGTT TCAGAAAGAA AGAAGAATTG
    GTAAAACTAG TAACTTTGGT TCATCACATG GGAGGTACTA TAAGGAAAGA CTTCAGTTCA
    AAGGTTACCC ATCTTATTGC CAATTCAACT CATGGAGATA AATTTAGGGT TGCAGTTAGT
    CTTGGAACTC CTATAATGAA GGCTGACTGG ATTATCCAAG CTTGGGAGAA AAGGAATGAA
    CTTGAATTCA GTGCAACAAA TGAAGAGTTC AAAAGTAAAT TTAAGGTTCC TCCGTTCCAA
    GACTGCATAT TAAGTTTTCT GGGTTTTTCT GATGAAGACA GAGTCAGCAT GGAAGAAATG
    ACTGAAATGC AAGGGGGAAC ATTTTTATCT GTTGGAGATG AGAAATGCAC TCATCTGGTT
    GTTGAAGAAA ATTCTGTGAA AGAGTTACCG TTTGAACCAC CTAAGAAACT GTACGTGGTA
    AAGCAAGAGT GGTTTTGGGG AAGTATCCAA ATGGATGCCA GAGCAGGAGA AACAATGTAC
    CTGTTTGAAA AGAATGATAG TCCAGCTCTC AAAAAATCTG TCTCTCTGCT CTCCTTGAAT
    ACTCCAAGCA GCAACCGAAA AAAACGTCGC TTAAAAGATA CGCTTGCTCA ATTAACCAGG
    GAGACAGATC TCACACCTTT TCCTCCTCGA AAACGTCCTT CAGCTGAACA TTCCCTTTCT
    ATAGGCTCAC TGCTAGATAT TTCAAATACC CCTGAGTCGG GAAAATCTTT TTCAGATACA
    CCAAAAATTT CAAAGCCTTC AAAAATATCT ACTCCTGTTC CTCAGCAATC AGCAAGGTGG
    CAAGTTGCTA TGGAACTGTA TCAAACTGAG AGTAATTATG TTGACATTCT AACTACAATT
    GTACAGCTTT TTCAACTTCC GTTGGAGAAA GAGGGACAAC TAGGAGGGCC AATTCTTGCT
    CCAGAAGAGA TCAAAACAAT ATTTGGCAGT ATCCCAGATA TTCTTGATGT CCACACTAAT
    ATAAAGGGAG ACCTGGAAAA ACTTATGATT GACTGGGCCG AAAGTAAAAG TATTGGGGAT
    ATTATCCTCA CCTATTCTAA AGATCTGGTG AAAATATATC CACCTTTTGT CAACTTCTTT
    GAAATGAGCA AGGAAATGAT TATAAAATGT GAAAAGCAGA AACCGAGATT TCACGCTTTT
    TTAAAGATCA ACCAGTCAAA GCCAGAATGT GGACGACAAA CTTTAGTCGA GCTCCTTATC
    AGACCTGTTC AACGGTTACC AAGTGTTGCA CTTCTTCTGA ATGATCTCAA AAAGCACACA
    GCAGATGATA ACCCAGACAA AGTAATGTTG GAAAAAGCAA TTGAATCATT AAAAGAAGTC
    ATGATGCACA TTAATGAAGA TAAAAGGAAG ACTGAAGGGC AAAAGCAGAT TTTTGATGTC
    GTCTATGAAG TGGATGGATG TCCTGCAAAT CTGTTATCTT CCCACCGAAT TTTAGTGCAG
    CGTGTAGAAA CAATTGCACT TGGTGAAGAT CTTTGTGATC GAGGAGAACA AGTTACACTT
    TTCCTGTTTA ATGATTGCCT TGAGATTGCA AGAAAACGGC ATAAGGGTAT AGGTGCATTC
    AAGAGTCCTC ATGGTACAAG ACCCCAAGCA TGCCTGAAAC ACATATATTT AATGCCTCTT
    TCACAAATTA AAAAAGTTCT TGACATCAAA GAAACTGAAG AATGTCATAA TGCATTTGCA
    TTGGTAGTGC GACCTCCAAC AGAACAGACA AACCAGTTAT TTAGTTTCCA GCTGACAACA
    GAGGAGCTTC CTAAGGTGGA TTGGCTGAAG ATGCTGTGCC GGCATGTAGC CAACACCATT
    TGTAAAGCAG ATGCTGAAAA TCTGATTTAT TCTACTGACC CTGATTCACT GGAAGTGAGC
    ACAAAGGATG TGGATAGTAC ATTAAGTAGA GCATCAAGGG CCATAAAGAA AACTTCTAAG
    AAAGTTACAA GAGCCTTTTC GTTCACCAAA ACACCCAAGA GAGCTCTTCA AAGGGCCCTG
    ATGGTACAAA ATGCAGATGG CCGAAGCCCT GGCCCAAGTA GTGAGGGTTT TGCAAGCTGC
    CGTATGCCAA GCACCACATC TCTGGCAATA TCTCGTTCCT CCTCTACCTT CAGCATTAAT
    GGACCAGGCA AACACCCTAG TGTTCAGCGA TCCAATTCTC TTGATGGGAG CCCTAGATCC
    CGTCCTGTTC TTTGCCCAGG CTCCCCTAGC AGCCCCTTGA CACATTCTCT AAAAGGGGTA
    GCTAATCAGT CCAGCCCTCC ATTATCTCCT TTCAGTGGGA AGTTTTGCAA AACCCTTGTT
    GTTCCACAAA CTAGAACATC AGGGTGTGCA GGCCAAAGTC AACAGGGCGC CGACCCTCCC
    TGCGTCAAGA AATCTTTGAG CATGAAAAGG GAGACTGCGC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818121.1
    CDS227..3091
    Translation

    Target ORF information:

    RefSeq Version XM_012962667.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962667.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGGAA ATCAAAGTAC CCTGCATCAA AACAGATTCT
    GTTGAAGAGT TTGTAGAAGC AGACACTCCA GAATTCGAAT CAGTGTTTGT TATATCAGAC
    TTTCAGAAAC CTGCTTATCG ATTTCTTTAC AAAGCTGACT GCAGAGTGCT TGGCCCCCCT
    ATTATAATTT ATTGTGCACA GAAAGGAGAG CCTTTGCCTT TTACATCCCG ACCTCTCTAC
    TGTGCAAGTA TGCTAAATCT CGTATTGTGC TTCACTGGTT TCAGAAAGAA AGAAGAATTG
    GTAAAACTAG TAACTTTGGT TCATCACATG GGAGGTACTA TAAGGAAAGA CTTCAGTTCA
    AAGGTTACCC ATCTTATTGC CAATTCAACT CATGGAGATA AATTTAGGGT TGCAGTTAGT
    CTTGGAACTC CTATAATGAA GGCTGACTGG ATTATCCAAG CTTGGGAGAA AAGGAATGAA
    CTTGAATTCA GTGCAACAAA TGAAGAGTTC AAAAGTAAAT TTAAGGTTCC TCCGTTCCAA
    GACTGCATAT TAAGTTTTCT GGGTTTTTCT GATGAAGACA GAGTCAGCAT GGAAGAAATG
    ACTGAAATGC AAGGGGGAAC ATTTTTATCT GTTGGAGATG AGAAATGCAC TCATCTGGTT
    GTTGAAGAAA ATTCTGTGAA AGAGTTACCG TTTGAACCAC CTAAGAAACT GTACGTGGTA
    AAGCAAGAGT GGTTTTGGGG AAGTATCCAA ATGGATGCCA GAGCAGGAGA AACAATGTAC
    CTGTTTGAAA AGAATGATAG TCCAGCTCTC AAAAAATCTG TCTCTCTGCT CTCCTTGAAT
    ACTCCAAGCA GCAACCGAAA AAAACGTCGC TTAAAAGATA CGCTTGCTCA ATTAACCAGG
    GAGACAGATC TCACACCTTT TCCTCCTCGA AAACGTCCTT CAGCTGAACA TTCCCTTTCT
    ATAGGCTCAC TGCTAGATAT TTCAAATACC CCTGAGTCGG GAAAATCTTT TTCAGATACA
    CCAAAAATTT CAAAGCCTTC AAAAATATCT ACTCCTGTTC CTCAGCAATC AGCAAGGTGG
    CAAGTTGCTA TGGAACTGTA TCAAACTGAG AGTAATTATG TTGACATTCT AACTACAATT
    GTACAGCTTT TTCAACTTCC GTTGGAGAAA GAGGGACAAC TAGGAGGGCC AATTCTTGCT
    CCAGAAGAGA TCAAAACAAT ATTTGGCAGT ATCCCAGATA TTCTTGATGT CCACACTAAT
    ATAAAGGGAG ACCTGGAAAA ACTTATGATT GACTGGGCCG AAAGTAAAAG TATTGGGGAT
    ATTATCCTCA CCTATTCTAA AGATCTGGTG AAAATATATC CACCTTTTGT CAACTTCTTT
    GAAATGAGCA AGGAAATGAT TATAAAATGT GAAAAGCAGA AACCGAGATT TCACGCTTTT
    TTAAAGATCA ACCAGTCAAA GCCAGAATGT GGACGACAAA CTTTAGTCGA GCTCCTTATC
    AGACCTGTTC AACGGTTACC AAGTGTTGCA CTTCTTCTGA ATGATCTCAA AAAGCACACA
    GCAGATGATA ACCCAGACAA AGTAATGTTG GAAAAAGCAA TTGAATCATT AAAAGAAGTC
    ATGATGCACA TTAATGAAGA TAAAAGGAAG ACTGAAGGGC AAAAGCAGAT TTTTGATGTC
    GTCTATGAAG TGGATGGATG TCCTGCAAAT CTGTTATCTT CCCACCGAAT TTTAGTGCAG
    CGTGTAGAAA CAATTGCACT TGGTGAAGAT CTTTGTGATC GAGGAGAACA AGTTACACTT
    TTCCTGTTTA ATGATTGCCT TGAGATTGCA AGAAAACGGC ATAAGGGTAT AGGTGCATTC
    AAGAGTCCTC ATGGTACAAG ACCCCAAGCA TGCCTGAAAC ACATATATTT AATGCCTCTT
    TCACAAATTA AAAAAGTTCT TGACATCAAA GAAACTGAAG AATGTCATAA TGCATTTGCA
    TTGGTAGTGC GACCTCCAAC AGAACAGACA AACCAGTTAT TTAGTTTCCA GCTGACAACA
    GAGGAGCTTC CTAAGGTGGA TTGGCTGAAG ATGCTGTGCC GGCATGTAGC CAACACCATT
    TGTAAAGCAG ATGCTGAAAA TCTGATTTAT TCTACTGACC CTGATTCACT GGAAGTGAGC
    ACAAAGGATG TGGATAGTAC ATTAAGTAGA GCATCAAGGG CCATAAAGAA AACTTCTAAG
    AAAGTTACAA GAGCCTTTTC GTTCACCAAA ACACCCAAGA GAGCTCTTCA AAGGGCCCTG
    ATGGTACAAA ATGCAGATGG CCGAAGCCCT GGCCCAAGTA GTGAGGGTTT TGCAAGCTGC
    CGTATGCCAA GCACCACATC TCTGGCAATA TCTCGTTCCT CCTCTACCTT CAGCATTAAT
    GGACCAGGCA AACACCCTAG TGTTCAGCGA TCCAATTCTC TTGATGGGAG CCCTAGATCC
    CGTCCTGTTC TTTGCCCAGG CTCCCCTAGC AGCCCCTTGA CACATTCTCT AAAAGGGGTA
    GCTAATCAGT CCAGCCCTCC ATTATCTCCT TTCAGTGGGA AGTTTTGCAA AACCCTTGTT
    GTTCCACAAA CTAGAACATC AGGGTGTGCA GGCCAAAGTC AACAGGGCGC CGACCCTCCC
    TGCGTCAAGA AATCTTTGAG CATGAAAAGG GAGACTGCGC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31070
    Clone ID Related Accession (Same CDS sequence) XM_012962668.2 , XM_012962668.3
    Accession Version XM_012962668.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962668.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    CATGTTCCTT CTCCGTCCTT GGTAAACCTG TCATCATTGT TTGAGAGGAA GTACCATACA
    TTTAGCCGAT CGACGACTCA CCTTATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818122.1
    CDS225..2954
    Translation

    Target ORF information:

    RefSeq Version XM_012962668.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962668.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    CATGTTCCTT CTCCGTCCTT GGTAAACCTG TCATCATTGT TTGAGAGGAA GTACCATACA
    TTTAGCCGAT CGACGACTCA CCTTATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31071
    Clone ID Related Accession (Same CDS sequence) XM_012962669.2 , XM_012962669.3
    Accession Version XM_012962669.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    TTGCTTATTC ATGGAGTGAG TACACCAACT ACATGTTGTG GCTTTTTCAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818123.1
    CDS226..2919
    Translation

    Target ORF information:

    RefSeq Version XM_012962669.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962669.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    TTGCTTATTC ATGGAGTGAG TACACCAACT ACATGTTGTG GCTTTTTCAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31068
    Clone ID Related Accession (Same CDS sequence) XM_012962666.2 , XM_012962664.2 , XM_012962665.2 , XM_012962664.3 , XM_031901485.1 , XM_012962663.2 , XM_012962663.3 , XM_012962665.3
    Accession Version XM_012962664.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962664.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818118.1
    CDS225..3182
    Translation

    Target ORF information:

    RefSeq Version XM_012962664.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962664.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31068
    Clone ID Related Accession (Same CDS sequence) XM_012962666.2 , XM_012962664.2 , XM_012962665.2 , XM_012962664.3 , XM_031901485.1 , XM_012962663.2 , XM_012962663.3 , XM_012962665.3
    Accession Version XM_031901485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757345.1
    CDS115..3072
    Translation

    Target ORF information:

    RefSeq Version XM_031901485.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31068
    Clone ID Related Accession (Same CDS sequence) XM_012962666.2 , XM_012962664.2 , XM_012962665.2 , XM_012962664.3 , XM_031901485.1 , XM_012962663.2 , XM_012962663.3 , XM_012962665.3
    Accession Version XM_012962663.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818117.1
    CDS225..3182
    Translation

    Target ORF information:

    RefSeq Version XM_012962663.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962663.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31068
    Clone ID Related Accession (Same CDS sequence) XM_012962666.2 , XM_012962664.2 , XM_012962665.2 , XM_012962664.3 , XM_031901485.1 , XM_012962663.2 , XM_012962663.3 , XM_012962665.3
    Accession Version XM_012962665.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962665.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818119.1
    CDS225..3182
    Translation

    Target ORF information:

    RefSeq Version XM_012962665.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962665.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    ATATCTCGTT CCTCCTCTAC CTTCAGCATT AATGGACCAG GCAAACACCC TAGTGTTCAG
    CGATCCAATT CTCTTGATGG GAGCCCTAGA TCCCGTCCTG TTCTTTGCCC AGGCTCCCCT
    AGCAGCCCCT TGACACATTC TCTAAAAGGG GTAGCTAATC AGTCCAGCCC TCCATTATCT
    CCTTTCAGTG GGAAGTTTTG CAAAACCCTT GTTGTTCCAC AAACTAGAAC ATCAGGGTGT
    GCAGGCCAAA GTCAACAGGG CGCCGACCCT CCCTGCGTCA AGAAATCTTT GAGCATGAAA
    AGGGAGACTG CGCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31069
    Clone ID Related Accession (Same CDS sequence) XM_012962667.2 , XM_012962667.3
    Accession Version XM_012962667.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2865bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGGAA ATCAAAGTAC CCTGCATCAA AACAGATTCT
    GTTGAAGAGT TTGTAGAAGC AGACACTCCA GAATTCGAAT CAGTGTTTGT TATATCAGAC
    TTTCAGAAAC CTGCTTATCG ATTTCTTTAC AAAGCTGACT GCAGAGTGCT TGGCCCCCCT
    ATTATAATTT ATTGTGCACA GAAAGGAGAG CCTTTGCCTT TTACATCCCG ACCTCTCTAC
    TGTGCAAGTA TGCTAAATCT CGTATTGTGC TTCACTGGTT TCAGAAAGAA AGAAGAATTG
    GTAAAACTAG TAACTTTGGT TCATCACATG GGAGGTACTA TAAGGAAAGA CTTCAGTTCA
    AAGGTTACCC ATCTTATTGC CAATTCAACT CATGGAGATA AATTTAGGGT TGCAGTTAGT
    CTTGGAACTC CTATAATGAA GGCTGACTGG ATTATCCAAG CTTGGGAGAA AAGGAATGAA
    CTTGAATTCA GTGCAACAAA TGAAGAGTTC AAAAGTAAAT TTAAGGTTCC TCCGTTCCAA
    GACTGCATAT TAAGTTTTCT GGGTTTTTCT GATGAAGACA GAGTCAGCAT GGAAGAAATG
    ACTGAAATGC AAGGGGGAAC ATTTTTATCT GTTGGAGATG AGAAATGCAC TCATCTGGTT
    GTTGAAGAAA ATTCTGTGAA AGAGTTACCG TTTGAACCAC CTAAGAAACT GTACGTGGTA
    AAGCAAGAGT GGTTTTGGGG AAGTATCCAA ATGGATGCCA GAGCAGGAGA AACAATGTAC
    CTGTTTGAAA AGAATGATAG TCCAGCTCTC AAAAAATCTG TCTCTCTGCT CTCCTTGAAT
    ACTCCAAGCA GCAACCGAAA AAAACGTCGC TTAAAAGATA CGCTTGCTCA ATTAACCAGG
    GAGACAGATC TCACACCTTT TCCTCCTCGA AAACGTCCTT CAGCTGAACA TTCCCTTTCT
    ATAGGCTCAC TGCTAGATAT TTCAAATACC CCTGAGTCGG GAAAATCTTT TTCAGATACA
    CCAAAAATTT CAAAGCCTTC AAAAATATCT ACTCCTGTTC CTCAGCAATC AGCAAGGTGG
    CAAGTTGCTA TGGAACTGTA TCAAACTGAG AGTAATTATG TTGACATTCT AACTACAATT
    GTACAGCTTT TTCAACTTCC GTTGGAGAAA GAGGGACAAC TAGGAGGGCC AATTCTTGCT
    CCAGAAGAGA TCAAAACAAT ATTTGGCAGT ATCCCAGATA TTCTTGATGT CCACACTAAT
    ATAAAGGGAG ACCTGGAAAA ACTTATGATT GACTGGGCCG AAAGTAAAAG TATTGGGGAT
    ATTATCCTCA CCTATTCTAA AGATCTGGTG AAAATATATC CACCTTTTGT CAACTTCTTT
    GAAATGAGCA AGGAAATGAT TATAAAATGT GAAAAGCAGA AACCGAGATT TCACGCTTTT
    TTAAAGATCA ACCAGTCAAA GCCAGAATGT GGACGACAAA CTTTAGTCGA GCTCCTTATC
    AGACCTGTTC AACGGTTACC AAGTGTTGCA CTTCTTCTGA ATGATCTCAA AAAGCACACA
    GCAGATGATA ACCCAGACAA AGTAATGTTG GAAAAAGCAA TTGAATCATT AAAAGAAGTC
    ATGATGCACA TTAATGAAGA TAAAAGGAAG ACTGAAGGGC AAAAGCAGAT TTTTGATGTC
    GTCTATGAAG TGGATGGATG TCCTGCAAAT CTGTTATCTT CCCACCGAAT TTTAGTGCAG
    CGTGTAGAAA CAATTGCACT TGGTGAAGAT CTTTGTGATC GAGGAGAACA AGTTACACTT
    TTCCTGTTTA ATGATTGCCT TGAGATTGCA AGAAAACGGC ATAAGGGTAT AGGTGCATTC
    AAGAGTCCTC ATGGTACAAG ACCCCAAGCA TGCCTGAAAC ACATATATTT AATGCCTCTT
    TCACAAATTA AAAAAGTTCT TGACATCAAA GAAACTGAAG AATGTCATAA TGCATTTGCA
    TTGGTAGTGC GACCTCCAAC AGAACAGACA AACCAGTTAT TTAGTTTCCA GCTGACAACA
    GAGGAGCTTC CTAAGGTGGA TTGGCTGAAG ATGCTGTGCC GGCATGTAGC CAACACCATT
    TGTAAAGCAG ATGCTGAAAA TCTGATTTAT TCTACTGACC CTGATTCACT GGAAGTGAGC
    ACAAAGGATG TGGATAGTAC ATTAAGTAGA GCATCAAGGG CCATAAAGAA AACTTCTAAG
    AAAGTTACAA GAGCCTTTTC GTTCACCAAA ACACCCAAGA GAGCTCTTCA AAGGGCCCTG
    ATGGTACAAA ATGCAGATGG CCGAAGCCCT GGCCCAAGTA GTGAGGGTTT TGCAAGCTGC
    CGTATGCCAA GCACCACATC TCTGGCAATA TCTCGTTCCT CCTCTACCTT CAGCATTAAT
    GGACCAGGCA AACACCCTAG TGTTCAGCGA TCCAATTCTC TTGATGGGAG CCCTAGATCC
    CGTCCTGTTC TTTGCCCAGG CTCCCCTAGC AGCCCCTTGA CACATTCTCT AAAAGGGGTA
    GCTAATCAGT CCAGCCCTCC ATTATCTCCT TTCAGTGGGA AGTTTTGCAA AACCCTTGTT
    GTTCCACAAA CTAGAACATC AGGGTGTGCA GGCCAAAGTC AACAGGGCGC CGACCCTCCC
    TGCGTCAAGA AATCTTTGAG CATGAAAAGG GAGACTGCGC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818121.1
    CDS225..3089
    Translation

    Target ORF information:

    RefSeq Version XM_012962667.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962667.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGGAA ATCAAAGTAC CCTGCATCAA AACAGATTCT
    GTTGAAGAGT TTGTAGAAGC AGACACTCCA GAATTCGAAT CAGTGTTTGT TATATCAGAC
    TTTCAGAAAC CTGCTTATCG ATTTCTTTAC AAAGCTGACT GCAGAGTGCT TGGCCCCCCT
    ATTATAATTT ATTGTGCACA GAAAGGAGAG CCTTTGCCTT TTACATCCCG ACCTCTCTAC
    TGTGCAAGTA TGCTAAATCT CGTATTGTGC TTCACTGGTT TCAGAAAGAA AGAAGAATTG
    GTAAAACTAG TAACTTTGGT TCATCACATG GGAGGTACTA TAAGGAAAGA CTTCAGTTCA
    AAGGTTACCC ATCTTATTGC CAATTCAACT CATGGAGATA AATTTAGGGT TGCAGTTAGT
    CTTGGAACTC CTATAATGAA GGCTGACTGG ATTATCCAAG CTTGGGAGAA AAGGAATGAA
    CTTGAATTCA GTGCAACAAA TGAAGAGTTC AAAAGTAAAT TTAAGGTTCC TCCGTTCCAA
    GACTGCATAT TAAGTTTTCT GGGTTTTTCT GATGAAGACA GAGTCAGCAT GGAAGAAATG
    ACTGAAATGC AAGGGGGAAC ATTTTTATCT GTTGGAGATG AGAAATGCAC TCATCTGGTT
    GTTGAAGAAA ATTCTGTGAA AGAGTTACCG TTTGAACCAC CTAAGAAACT GTACGTGGTA
    AAGCAAGAGT GGTTTTGGGG AAGTATCCAA ATGGATGCCA GAGCAGGAGA AACAATGTAC
    CTGTTTGAAA AGAATGATAG TCCAGCTCTC AAAAAATCTG TCTCTCTGCT CTCCTTGAAT
    ACTCCAAGCA GCAACCGAAA AAAACGTCGC TTAAAAGATA CGCTTGCTCA ATTAACCAGG
    GAGACAGATC TCACACCTTT TCCTCCTCGA AAACGTCCTT CAGCTGAACA TTCCCTTTCT
    ATAGGCTCAC TGCTAGATAT TTCAAATACC CCTGAGTCGG GAAAATCTTT TTCAGATACA
    CCAAAAATTT CAAAGCCTTC AAAAATATCT ACTCCTGTTC CTCAGCAATC AGCAAGGTGG
    CAAGTTGCTA TGGAACTGTA TCAAACTGAG AGTAATTATG TTGACATTCT AACTACAATT
    GTACAGCTTT TTCAACTTCC GTTGGAGAAA GAGGGACAAC TAGGAGGGCC AATTCTTGCT
    CCAGAAGAGA TCAAAACAAT ATTTGGCAGT ATCCCAGATA TTCTTGATGT CCACACTAAT
    ATAAAGGGAG ACCTGGAAAA ACTTATGATT GACTGGGCCG AAAGTAAAAG TATTGGGGAT
    ATTATCCTCA CCTATTCTAA AGATCTGGTG AAAATATATC CACCTTTTGT CAACTTCTTT
    GAAATGAGCA AGGAAATGAT TATAAAATGT GAAAAGCAGA AACCGAGATT TCACGCTTTT
    TTAAAGATCA ACCAGTCAAA GCCAGAATGT GGACGACAAA CTTTAGTCGA GCTCCTTATC
    AGACCTGTTC AACGGTTACC AAGTGTTGCA CTTCTTCTGA ATGATCTCAA AAAGCACACA
    GCAGATGATA ACCCAGACAA AGTAATGTTG GAAAAAGCAA TTGAATCATT AAAAGAAGTC
    ATGATGCACA TTAATGAAGA TAAAAGGAAG ACTGAAGGGC AAAAGCAGAT TTTTGATGTC
    GTCTATGAAG TGGATGGATG TCCTGCAAAT CTGTTATCTT CCCACCGAAT TTTAGTGCAG
    CGTGTAGAAA CAATTGCACT TGGTGAAGAT CTTTGTGATC GAGGAGAACA AGTTACACTT
    TTCCTGTTTA ATGATTGCCT TGAGATTGCA AGAAAACGGC ATAAGGGTAT AGGTGCATTC
    AAGAGTCCTC ATGGTACAAG ACCCCAAGCA TGCCTGAAAC ACATATATTT AATGCCTCTT
    TCACAAATTA AAAAAGTTCT TGACATCAAA GAAACTGAAG AATGTCATAA TGCATTTGCA
    TTGGTAGTGC GACCTCCAAC AGAACAGACA AACCAGTTAT TTAGTTTCCA GCTGACAACA
    GAGGAGCTTC CTAAGGTGGA TTGGCTGAAG ATGCTGTGCC GGCATGTAGC CAACACCATT
    TGTAAAGCAG ATGCTGAAAA TCTGATTTAT TCTACTGACC CTGATTCACT GGAAGTGAGC
    ACAAAGGATG TGGATAGTAC ATTAAGTAGA GCATCAAGGG CCATAAAGAA AACTTCTAAG
    AAAGTTACAA GAGCCTTTTC GTTCACCAAA ACACCCAAGA GAGCTCTTCA AAGGGCCCTG
    ATGGTACAAA ATGCAGATGG CCGAAGCCCT GGCCCAAGTA GTGAGGGTTT TGCAAGCTGC
    CGTATGCCAA GCACCACATC TCTGGCAATA TCTCGTTCCT CCTCTACCTT CAGCATTAAT
    GGACCAGGCA AACACCCTAG TGTTCAGCGA TCCAATTCTC TTGATGGGAG CCCTAGATCC
    CGTCCTGTTC TTTGCCCAGG CTCCCCTAGC AGCCCCTTGA CACATTCTCT AAAAGGGGTA
    GCTAATCAGT CCAGCCCTCC ATTATCTCCT TTCAGTGGGA AGTTTTGCAA AACCCTTGTT
    GTTCCACAAA CTAGAACATC AGGGTGTGCA GGCCAAAGTC AACAGGGCGC CGACCCTCCC
    TGCGTCAAGA AATCTTTGAG CATGAAAAGG GAGACTGCGC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31070
    Clone ID Related Accession (Same CDS sequence) XM_012962668.2 , XM_012962668.3
    Accession Version XM_012962668.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962668.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    CATGTTCCTT CTCCGTCCTT GGTAAACCTG TCATCATTGT TTGAGAGGAA GTACCATACA
    TTTAGCCGAT CGACGACTCA CCTTATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818122.1
    CDS224..2953
    Translation

    Target ORF information:

    RefSeq Version XM_012962668.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962668.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    CATGTTCCTT CTCCGTCCTT GGTAAACCTG TCATCATTGT TTGAGAGGAA GTACCATACA
    TTTAGCCGAT CGACGACTCA CCTTATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31071
    Clone ID Related Accession (Same CDS sequence) XM_012962669.2 , XM_012962669.3
    Accession Version XM_012962669.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein ECT2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962669.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    TTGCTTATTC ATGGAGTGAG TACACCAACT ACATGTTGTG GCTTTTTCAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818123.1
    CDS224..2917
    Translation

    Target ORF information:

    RefSeq Version XM_012962669.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis epithelial cell transforming 2 (ect2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962669.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAATGACA ACAGCATGCT GGCACCCGCT GCGGGAAAGA GTCTTCTAGC GGACTCCTCT 
    GTGTTTGACT CTAAAATCAC AGAGACATCC AAAGATAACT TGTTTGCTGG CTCTGCAGAT
    ATTGACGAAG AAATGCCCCA GATTGAAACC AGAGTGGTTT TAGTTGAAGA CGCTGGAAGA
    AACGACGAGT TAATAAAAGC TCTAGAGACG GTAAAAGTAA TGGAAGTTCC AGTTATAAAG
    ATAAAGGAAA ACTGTCCTGA AAAATCCAGG GAATCTTTCA TAAAAAGTAT TGTTAATATG
    GAAATCAAAG TACCCTGCAT CAAAACAGAT TCTGTTGAAG AGTTTGTAGA AGCAGACACT
    CCAGAATTCG AATCAGTGTT TGTTATATCA GACTTTCAGA AACCTGCTTA TCGATTTCTT
    TACAAAGCTG ACTGCAGAGT GCTTGGCCCC CCTATTATAA TTTATTGTGC ACAGAAAGGA
    GAGCCTTTGC CTTTTACATC CCGACCTCTC TACTGTGCAA GTATGCTAAA TCTCGTATTG
    TGCTTCACTG GTTTCAGAAA GAAAGAAGAA TTGGTAAAAC TAGTAACTTT GGTTCATCAC
    ATGGGAGGTA CTATAAGGAA AGACTTCAGT TCAAAGGTTA CCCATCTTAT TGCCAATTCA
    ACTCATGGAG ATAAATTTAG GGTTGCAGTT AGTCTTGGAA CTCCTATAAT GAAGGCTGAC
    TGGATTATCC AAGCTTGGGA GAAAAGGAAT GAACTTGAAT TCAGTGCAAC AAATGAAGAG
    TTCAAAAGTA AATTTAAGGT TCCTCCGTTC CAAGACTGCA TATTAAGTTT TCTGGGTTTT
    TCTGATGAAG ACAGAGTCAG CATGGAAGAA ATGACTGAAA TGCAAGGGGG AACATTTTTA
    TCTGTTGGAG ATGAGAAATG CACTCATCTG GTTGTTGAAG AAAATTCTGT GAAAGAGTTA
    CCGTTTGAAC CACCTAAGAA ACTGTACGTG GTAAAGCAAG AGTGGTTTTG GGGAAGTATC
    CAAATGGATG CCAGAGCAGG AGAAACAATG TACCTGTTTG AAAAGAATGA TAGTCCAGCT
    CTCAAAAAAT CTGTCTCTCT GCTCTCCTTG AATACTCCAA GCAGCAACCG AAAAAAACGT
    CGCTTAAAAG ATACGCTTGC TCAATTAACC AGGGAGACAG ATCTCACACC TTTTCCTCCT
    CGAAAACGTC CTTCAGCTGA ACATTCCCTT TCTATAGGCT CACTGCTAGA TATTTCAAAT
    ACCCCTGAGT CGGGAAAATC TTTTTCAGAT ACACCAAAAA TTTCAAAGCC TTCAAAAATA
    TCTACTCCTG TTCCTCAGCA ATCAGCAAGG TGGCAAGTTG CTATGGAACT GTATCAAACT
    GAGAGTAATT ATGTTGACAT TCTAACTACA ATTGTACAGC TTTTTCAACT TCCGTTGGAG
    AAAGAGGGAC AACTAGGAGG GCCAATTCTT GCTCCAGAAG AGATCAAAAC AATATTTGGC
    AGTATCCCAG ATATTCTTGA TGTCCACACT AATATAAAGG GAGACCTGGA AAAACTTATG
    ATTGACTGGG CCGAAAGTAA AAGTATTGGG GATATTATCC TCACCTATTC TAAAGATCTG
    GTGAAAATAT ATCCACCTTT TGTCAACTTC TTTGAAATGA GCAAGGAAAT GATTATAAAA
    TGTGAAAAGC AGAAACCGAG ATTTCACGCT TTTTTAAAGA TCAACCAGTC AAAGCCAGAA
    TGTGGACGAC AAACTTTAGT CGAGCTCCTT ATCAGACCTG TTCAACGGTT ACCAAGTGTT
    GCACTTCTTC TGAATGATCT CAAAAAGCAC ACAGCAGATG ATAACCCAGA CAAAGTAATG
    TTGGAAAAAG CAATTGAATC ATTAAAAGAA GTCATGATGC ACATTAATGA AGATAAAAGG
    AAGACTGAAG GGCAAAAGCA GATTTTTGAT GTCGTCTATG AAGTGGATGG ATGTCCTGCA
    AATCTGTTAT CTTCCCACCG AATTTTAGTG CAGCGTGTAG AAACAATTGC ACTTGGTGAA
    GATCTTTGTG ATCGAGGAGA ACAAGTTACA CTTTTCCTGT TTAATGATTG CCTTGAGATT
    GCAAGAAAAC GGCATAAGGG TATAGGTGCA TTCAAGAGTC CTCATGGTAC AAGACCCCAA
    GCATGCCTGA AACACATATA TTTAATGCCT CTTTCACAAA TTAAAAAAGT TCTTGACATC
    AAAGAAACTG AAGAATGTCA TAATGCATTT GCATTGGTAG TGCGACCTCC AACAGAACAG
    ACAAACCAGT TATTTAGTTT CCAGCTGACA ACAGAGGAGC TTCCTAAGGT GGATTGGCTG
    AAGATGCTGT GCCGGCATGT AGCCAACACC ATTTGTAAAG CAGATGCTGA AAATCTGATT
    TATTCTACTG ACCCTGATTC ACTGGAAGTG AGCACAAAGG ATGTGGATAG TACATTAAGT
    AGAGCATCAA GGGCCATAAA GAAAACTTCT AAGAAAGTTA CAAGAGCCTT TTCGTTCACC
    AAAACACCCA AGAGAGCTCT TCAAAGGGCC CTGATGGTAC AAAATGCAGA TGGCCGAAGC
    CCTGGCCCAA GTAGTGAGGG TTTTGCAAGC TGCCGTATGC CAAGCACCAC ATCTCTGGCA
    TTGCTTATTC ATGGAGTGAG TACACCAACT ACATGTTGTG GCTTTTTCAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.