NCL cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCL
Entrez Gene ID	497013
Full Name	nucleolin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001206660.1	NP_001193589.1	nucleolin

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ncl	NP_036881.2
Canis lupus familiaris (dog)	NCL	XP_850477.2
Mus musculus (house mouse)	Ncl	NP_035010.3
Bos taurus (cattle)	NCL	NP_001193589.1
Homo sapiens (human)	NCL	NP_005372.2
Pan troglodytes (chimpanzee)	NCL	XP_516145.3
	LOC720965	XP_001116949.2

Gene Ontology
Bibliography

Related articles in PubMed

RNA-mediated gene silencing of FUT1 and FUT2 influences expression and activities of bovine and human fucosylated nucleolin and inhibits cell adhesion and proliferation.
Palumberi D, Aldi S, Ermini L, Ziche M, Finetti F, Donnini S, Rosati F
Journal of cellular biochemistry111(1)229-38(2010 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that si-RNA induced down-regulation of FUT1 and FUT2 reduced expression of fucosylated nucleolin glycoforms and their exposure at the cell surface.
Title: RNA-mediated gene silencing of FUT1 and FUT2 influences expression and activities of bovine and human fucosylated nucleolin and inhibits cell adhesion and proliferation.

The following NCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa37652	NM_001206660.1 Latest version!	Bos taurus nucleolin (NCL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCL ( NM_001206660.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193589.1 Bos taurus nucleolin (NCL), mRNA.

Price & Availability↑

CloneID	OBa37652
Clone ID Related Accession (Same CDS sequence)	NM_001206660.1
Accession Version	NM_001206660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	nucleolin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV807609.1, EV621481.1, EH209294.1, DV910666.1 and EV700525.1. On or before May 4, 2011 this sequence version replaced XM_002685596.1, XM_614626.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTAAAGC TCGCAAAGGC CGGTAAAAAT CAAGGTGACT CCAAGAAAAT GGCTCCTCCC 
CCAAAGGAGG TAGAAGAAGA TAGTGAAGAT GAGGAAATGT CAGAAGATGA AGACGATGAG 
AGCAGTGGAG AAGAGGTTGT TATCCCTCAG AAAAAAGGCA AGAAGGCTGC CACAACTCCA 
GCAAAGAAGA TGGTGGTTTC CCCAACAAAA AAGGTTGCAG TTGCCACACC AGCAAAGAAA 
GCAGTTGTCA CCCCTGGCAA AAAGGCAGCA GCTATGCCAG CCAAGAAGAC AGTTACACCT 
GCCAAAGCAG TGGTTACACC TGGCAAAAAG GGAGCCACCC CAGGCAAAGC AGTGGTAGCA 
ACCTCTGGTA AGAAGGGAGC AGCCACCCCA GGCAAGGGAG CAAAGAACGG CAAGAATGCC 
AAGAAGGAAG ACAGTGATGA AGAAGATGAA GATGACAGTG AAGAGGAAGA CGATGATGAT 
GAAGAGGAGG ATGAGCCAGC AGTGAGGAAG GCAGCTGCTG CTTTTGGTGC TGCTCCTGCC 
ACAGATGATG AGGATGACGA GGATGACGAT GATGACGACG ATGAGGAGGA GGAGGAGGAT 
GAGGAGGATG AGGAGGACGA AGATGACTCT GAAGAAGAAC CTATGGAGAT TGTACCAGCC 
AAAGGAAAGA AAGCTCCAGT AAAAGCTGTT CCTGTGAAGG CTAAGAGCAC TGCTGAAGAT 
GAAGATGAAG AGGATGATGA TGATGATGAT GATGAAGAGG AGGAGGAAGA GGATGACGAT 
GATGAAGACG ACGACGATGA GGAGGAAGAG GAGGAGGAGG AAGAGGAGGA AGAGGAAGAA 
GAGCCTGTCA AAGAAGCACC TGGAAAACGA AAGAAGGAAA TGGCCAAACA AAAAGCAGCT 
CCTGAAGCCA AGAAACAAAA AGTGGAAGGC ACAGAACCAA CTACATCTTT CAATCTCTTT 
GTTGGAAATC TGAACTTCAA TAAATCTGCT CCTGAATTGA AAACGGGTAT CAGTGATCTT 
TTTGCTAAAA ATGATCTTGC TGTTGTTGAT GTCAGAATTG GTGTGTCTAG GAAGTTTGGC 
TATGTGGATT TTGAATCTGC CGAAGACCTG GAAAAAGCCT TGGAACTCAC TGGTTTAAAA 
GTCTTTGGCA ATGAAATTAA ACTAGAAAAA CCAAAGGGAA AAGACAGTAA GAAAGATCGA 
GATGCAAGAA CACTTCTGGC TAAAAATCTG CCTTACAAAG TTACTCAGGA TGAATTAAAA 
GAAGTGTTTG AAGATGCTGT GGAGATCAGA TTAGTCAGCA AGGATGGAAA GAGTAAAGGG 
ATTGCTTATA TTGAATTTAA GACAGAAGCT GATGCAGAAA AAACCTTGGA AGAAAAGCAG 
GGAACAGAGA TAGATGGGCG ATCCATTTCT CTGTACTACA CCGGAGAGAA AGGTCAAAGT 
CAAGACCATA GAGGTGGAAA GAACAGCACT TGGAGTGGTG AATCAAAAAC CCTGGTTTTA 
AGCAATCTCT CCTATAGTGC AACGGAAGAA ACTCTTCAGG AAGTATTTGA GAAGGCAACT 
CATATCAAGG TGCCCCAGAA CCAAAATGGC AAATCTAAAG GGTATGCATT TATAGAATTT 
GCTTCATTTG AAGATGCTAA AGAAGCTTTA AATTCATGTA ATAAAAGGGA AATTGAGGGC 
AGAGCCATCA GACTGGAGTT GCAAGGACCC AGGGGATCAC CTAATGCAAG AAGCCAGCCA 
TCCAAAACTC TCTTTGTCAA AGGTCTCTCT GAGGATACCA CAGAAGAGAC GTTAAAAGAG 
TCGTTTGATG GCTCTATTCG AGCAAGGATA GTCACTGACC GGGAGACTGG ATCCTCCAAA 
GGGTTTGGTT TTGTAGACTT CAACAGCGAG GAAGATGCCA AAGCTGCCAA GGAGGCCATG 
GAAGATGGTG AAATCGATGG AAACAAAGTC ACTTTGGACT GGGCCAAACC TAAGGGTGAA 
GGTGGCTTTG GTGGCCGAGG AGGAGGCAGA GGTGGCTTTG GAGGCCGAGG TGGTGGCAGA 
GGTGGCCGAG GTGGCTTTGG TGGCAGAGGC CGGGGAGGCT TTGGAGGGCG AGGAGGCTTC 
CGAGGAGGCA GAGGAGGAGG AGGAGACCAC AAGCCACAAG GAAAGAAGAC GAAGTTTGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193589.1
CDS	163..2325
Translation	MVKLAKAGKNQGDSKKMAPPPKEVEEDSEDEEMSEDEDDESSGEEVVIPQKKGKKAATTPAKKMVVSPTKKVAVATPAKKAVVTPGKKAAAMPAKKTVTPAKAVVTPGKKGATPGKAVVATSGKKGAATPGKGAKNGKNAKKEDSDEEDEDDSEEEDDDDEEEDEPAVRKAAAAFGAAPATDDEDDEDDDDDDDEEEEEDEEDEEDEDDSEEEPMEIVPAKGKKAPVKAVPVKAKSTAEDEDEEDDDDDDDEEEEEEDDDDEDDDDEEEEEEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGTEPTTSFNLFVGNLNFNKSAPELKTGISDLFAKNDLAVVDVRIGVSRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKDRDARTLLAKNLPYKVTQDELKEVFEDAVEIRLVSKDGKSKGIAYIEFKTEADAEKTLEEKQGTEIDGRSISLYYTGEKGQSQDHRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATHIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSIRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE

Target ORF information:

RefSeq Version	NM_001206660.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nucleolin (NCL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206660.1

ORF Insert Sequence:

ATGGTAAAGC TCGCAAAGGC CGGTAAAAAT CAAGGTGACT CCAAGAAAAT GGCTCCTCCC 
CCAAAGGAGG TAGAAGAAGA TAGTGAAGAT GAGGAAATGT CAGAAGATGA AGACGATGAG 
AGCAGTGGAG AAGAGGTTGT TATCCCTCAG AAAAAAGGCA AGAAGGCTGC CACAACTCCA 
GCAAAGAAGA TGGTGGTTTC CCCAACAAAA AAGGTTGCAG TTGCCACACC AGCAAAGAAA 
GCAGTTGTCA CCCCTGGCAA AAAGGCAGCA GCTATGCCAG CCAAGAAGAC AGTTACACCT 
GCCAAAGCAG TGGTTACACC TGGCAAAAAG GGAGCCACCC CAGGCAAAGC AGTGGTAGCA 
ACCTCTGGTA AGAAGGGAGC AGCCACCCCA GGCAAGGGAG CAAAGAACGG CAAGAATGCC 
AAGAAGGAAG ACAGTGATGA AGAAGATGAA GATGACAGTG AAGAGGAAGA CGATGATGAT 
GAAGAGGAGG ATGAGCCAGC AGTGAGGAAG GCAGCTGCTG CTTTTGGTGC TGCTCCTGCC 
ACAGATGATG AGGATGACGA GGATGACGAT GATGACGACG ATGAGGAGGA GGAGGAGGAT 
GAGGAGGATG AGGAGGACGA AGATGACTCT GAAGAAGAAC CTATGGAGAT TGTACCAGCC 
AAAGGAAAGA AAGCTCCAGT AAAAGCTGTT CCTGTGAAGG CTAAGAGCAC TGCTGAAGAT 
GAAGATGAAG AGGATGATGA TGATGATGAT GATGAAGAGG AGGAGGAAGA GGATGACGAT 
GATGAAGACG ACGACGATGA GGAGGAAGAG GAGGAGGAGG AAGAGGAGGA AGAGGAAGAA 
GAGCCTGTCA AAGAAGCACC TGGAAAACGA AAGAAGGAAA TGGCCAAACA AAAAGCAGCT 
CCTGAAGCCA AGAAACAAAA AGTGGAAGGC ACAGAACCAA CTACATCTTT CAATCTCTTT 
GTTGGAAATC TGAACTTCAA TAAATCTGCT CCTGAATTGA AAACGGGTAT CAGTGATCTT 
TTTGCTAAAA ATGATCTTGC TGTTGTTGAT GTCAGAATTG GTGTGTCTAG GAAGTTTGGC 
TATGTGGATT TTGAATCTGC CGAAGACCTG GAAAAAGCCT TGGAACTCAC TGGTTTAAAA 
GTCTTTGGCA ATGAAATTAA ACTAGAAAAA CCAAAGGGAA AAGACAGTAA GAAAGATCGA 
GATGCAAGAA CACTTCTGGC TAAAAATCTG CCTTACAAAG TTACTCAGGA TGAATTAAAA 
GAAGTGTTTG AAGATGCTGT GGAGATCAGA TTAGTCAGCA AGGATGGAAA GAGTAAAGGG 
ATTGCTTATA TTGAATTTAA GACAGAAGCT GATGCAGAAA AAACCTTGGA AGAAAAGCAG 
GGAACAGAGA TAGATGGGCG ATCCATTTCT CTGTACTACA CCGGAGAGAA AGGTCAAAGT 
CAAGACCATA GAGGTGGAAA GAACAGCACT TGGAGTGGTG AATCAAAAAC CCTGGTTTTA 
AGCAATCTCT CCTATAGTGC AACGGAAGAA ACTCTTCAGG AAGTATTTGA GAAGGCAACT 
CATATCAAGG TGCCCCAGAA CCAAAATGGC AAATCTAAAG GGTATGCATT TATAGAATTT 
GCTTCATTTG AAGATGCTAA AGAAGCTTTA AATTCATGTA ATAAAAGGGA AATTGAGGGC 
AGAGCCATCA GACTGGAGTT GCAAGGACCC AGGGGATCAC CTAATGCAAG AAGCCAGCCA 
TCCAAAACTC TCTTTGTCAA AGGTCTCTCT GAGGATACCA CAGAAGAGAC GTTAAAAGAG 
TCGTTTGATG GCTCTATTCG AGCAAGGATA GTCACTGACC GGGAGACTGG ATCCTCCAAA 
GGGTTTGGTT TTGTAGACTT CAACAGCGAG GAAGATGCCA AAGCTGCCAA GGAGGCCATG 
GAAGATGGTG AAATCGATGG AAACAAAGTC ACTTTGGACT GGGCCAAACC TAAGGGTGAA 
GGTGGCTTTG GTGGCCGAGG AGGAGGCAGA GGTGGCTTTG GAGGCCGAGG TGGTGGCAGA 
GGTGGCCGAG GTGGCTTTGG TGGCAGAGGC CGGGGAGGCT TTGGAGGGCG AGGAGGCTTC 
CGAGGAGGCA GAGGAGGAGG AGGAGACCAC AAGCCACAAG GAAAGAAGAC GAAGTTTGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA-mediated gene silencing of FUT1 and FUT2 influences expression and activities of bovine and human fucosylated nucleolin and inhibits cell adhesion and proliferation.
Journal of cellular biochemistry111(1)229-38(2010 Sep)
Palumberi D,Aldi S,Ermini L,Ziche M,Finetti F,Donnini S,Rosati F

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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NCL cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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