EPAS1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	EPAS1
Entrez Gene ID	282711
Full Name	endothelial PAS domain protein 1
General protein information	Preferred Names endothelial PAS domain protein 1 Names endothelial PAS domain-containing protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174725.2	NP_777150.1	endothelial PAS domain-containing protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EPAS1	XP_005626137.1
Rattus norvegicus (Norway rat)	Epas1	NP_075578.1
	EPAS1	XP_001112947.2
Mus musculus (house mouse)	Epas1	NP_034267.3
Pan troglodytes (chimpanzee)	EPAS1	XP_001147219.1
Gallus gallus (chicken)	EPAS1	NP_990138.1
Danio rerio (zebrafish)	epas1b	NP_001034895.1
Xenopus tropicalis (tropical clawed frog)	epas1	NP_001005647.1
Homo sapiens (human)	EPAS1	NP_001421.2
Bos taurus (cattle)	EPAS1	NP_777150.1

Gene Ontology
Bibliography

Related articles in PubMed

Increased prevalence of EPAS1 variant in cattle with high-altitude pulmonary hypertension.
Newman JH, Holt TN, Cogan JD, Womack B, Phillips JA 3rd, Li C, Kendall Z, Stenmark KR, Thomas MG, Brown RD, Riddle SR, West JD, Hamid R
Nature communications66863(2015 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

Molecular cloning of cDNAs encoding hypoxia-inducible factor (HIF)-1alpha and -2alpha of bovine arterial endothelial cells.
Hara S, Kobayashi C, Imura N
Biochimica et biophysica acta1445(2)237-43(1999 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

There was an association of an EPAS1 (HIF2alpha) double variant in the oxygen degradation domain of EPAS1 in Angus cattle with high altitude pulmonary hypertension (HAPH). There was upregulation of 26 of 27 HIF2alpha target genes in EPAS1 carriers with HAPH.
Title: Increased prevalence of EPAS1 variant in cattle with high-altitude pulmonary hypertension.

The following EPAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa37604	NM_174725.2 Latest version!	Bos taurus endothelial PAS domain protein 1 (EPAS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPAS1 ( NM_174725.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777150.1 Bos taurus endothelial PAS domain protein 1 (EPAS1), mRNA.

Price & Availability↑

CloneID	OBa37604
Clone ID Related Accession (Same CDS sequence)	NM_174725.2
Accession Version	NM_174725.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	endothelial PAS domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB018399.1. On Jun 3, 2003 this sequence version replaced NM_174725.1. ##Evidence-Data-START## Transcript exon combination :: AB018399.1, BC142229.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGACAGCTG ACAAGGAGAA GAAAAGGAGC AGCTCTGAGA GGAGGAAGGA GAAGTCCCGA 
GATGCAGCCA GGTGCCGGCG GAGCAAGGAA ACAGAGGTCT TCTATGAGCT GGCGCACGAG 
CTGCCCCTGC CGCACAGCGT GAGCTCGCAC CTGGACAAGG CCTCCATCAT GCGGCTGGCC 
ATCAGCTTCC TGCGCACGCA CAAGCTCCTG TCCTCTGTTT GCTCTGAAAA TGAATCTGAA 
GCCGACGCTG ACCAGCAGAT GGACAACTTG TATCTGAAAG CCTTGGAGGG TTTCATTGCC 
GTGGTGACCC AAGATGGTGA CATGATCTTT CTGTCGGAGA ACATCAGCAA GTTCATGGGA 
CTCACACAGG TAGAGTTAAC AGGACACAGT ATCTTTGACT TCACTCATCC CTGTGACCAT 
GAGGAGATCC GTGAGAACTT GAGCCTCAAA AATGGCTCTG GTTTTGGGAA GAAAAGCAAA 
GACATGTCCA CAGAGCGGGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC 
CGCACAGTCA ACCTCAAGTC AGCCACCTGG AAGGTCTTGC ACTGCACGGG CCAGGTGAAG 
GTCTACAACA ACTGCCCCCC TCACAGCAGT CTCTGCGGCT GCAAGGAACC GCTGCTGTCC 
TGCCTCATCA TCATGTGTGA GCCGATCCAG CACCCATCCC ACATGGACAT CCCACTGGAC 
AGCAAGACCT TCCTGAGCCG CCACAGTATG GACATGAAGT TCACCTACTG CGACGACAGA 
ATCACAGAAC TGGTTGGTTA CCACCCTGAA GAGCTGCTTG GCCGCTCAGC CTATGAGTTC 
TACCATGCAC TGGACTCAGA GAACATGACC AAAAGCCACC AGAACTTGTG CACCAAGGGT 
CAGGTGGTGA GCGGCCAGTA CCGGATGCTT GCCAAGCACG GGGGCTATGT GTGGCTGGAG 
ACACAGGGGA CGGTGATCTA CAACCCTCGC AACCTGCAGC CCCAGTGCAT CATGTGTGTG 
AACTACGTCC TGAGTGAAAT TGAGAAGAAC GACGTGGTGT TCTCCATGGA TCAGACAGAG 
TCACTGTTTA AGCCGCACCT GCTGACCATG AACAGCATCT TTGATAACAG TGGCAAGGTG 
GCTGTGTCTG AGAAGAGCAA CTTCCTATTC ACCAAGCTGA AGGAGGAGCC CGAGGAGCTG 
GCCCAGCTGG CACCTACCGC GGGAGATACC ATCATTTCTC TGGATTTCGG GACCCCGAAC 
TTTGAGGAGT CCTCAGCCTA TGGCAAGGGC ATCCTGCCCC CAGGCCAGCA GTGGACCGGA 
GAGGTGAAGA GCCACGGCAC CCACAGTGAG GCCGGGAGCC TGCCCGCCTT CACTGTGCCC 
CAGGCGGCCG CCCTGGGGAA TAGCACCCCC AGTGCCAGCA GCAGCAGCAG CTGCTCCACG 
CCCAGTAGCC CTGGAGACTA TTACACATCT CTAGATGACA ATCTGAAGAT TGAAGCAATC 
GAGAAGCTCT TTGCCATGGA CACAGAGGCG AAGGACCAGT GTGGCACCCA GACTGACTTT 
AACGAGCTGG ATTTGGAGAC CCTGGCACCT TACATCCCCA TGGACGGAGA GGACTTCCAG 
CTTAGCCCCA TCTGCCCTGA GGAGAGCCTC CTGCCGGAGA CCCCCCAGTC GGCCCCCCAG 
CACTGCTTCA GCACCATGTC AAACATCTTC CAGCCACTGG CTCCGATGGC CTCTCACAGC 
ACCTTCCTCC TGGACAAGTA TCAGCAGCAG CTGGAAAGCA AGAAGACGGA GCCTGAGCAG 
CGGCGTGTGT CCTTCGCCTT CTTTGACGGT GGGAGCAGGG TGTCCCTGCT GCAGTGCTGT 
GGTCAGACCT ACACCCCCCT CTCCTCCATG GGGGGCATTT CCAACACCCA GTGGCCCCCT 
GACCCACCAC TACAGCTGGG GCCCACGAAG TGGCCTGGTG AAGACCGGCA CGCAGAGGCC 
GTGGGGGCAG CGCCCCTGGG GCTCCCCCCC GCCACACCCC ATCTCGCCAT GCTCAAGAAG 
AGGTCTGCCA AGGGCTTCGG GCCTCAGGGT CCAGACGTGA TGAGCCCAGC CATGATTGCC 
CTCTCCAACA AGCTGAAGCT GAAGCGACAG CTGGAGTACG AGGAGCAAGC CTTCCAAGAC 
ATGAGTGGGG GGGATCCGCC AGGCAGCGGC ACCTCGCATT TGATGTGGAA AAGAATGAAG 
AGTCTCAGGG GTGGTGGGAC CTGCTCTTTG ATGCCGGACA AGCTGCCAAA TGCAAATGTC 
CCTAATGATG AGTTCATCCA GAATCCCGTG AGGGGTAGGA GCCAGCCCCT GAGACACTTA 
TCACCACCAC AGCCTCCGTC TGCCACGAGC CCGGGGGAGC CCACCAAGAG TGGGTTCCCC 
GCTCAGTGTT ACGCCCCCCA GTACCAGGAC TACAGCCTAC CCGCGGCTCA CAAGATGTCA 
GGCATGGCAA GCCGGCTGCT CGGACCCTCG TTTGAGCCCT ACCTGCTGCC GGAACTGACC 
AGATATGACT GTGAGGTGAA CGTGCCTGTT CCGGGAACCT CCACGCTCCT CCAAGGAGGG 
GATCTCCTCC GAGCCCTGGA CCAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777150.1
CDS	274..2886
Translation	MTADKEKKRSSSERRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEADADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSKDMSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVKVYNNCPPHSSLCGCKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELVGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKNDVVFSMDQTESLFKPHLLTMNSIFDNSGKVAVSEKSNFLFTKLKEEPEELAQLAPTAGDTIISLDFGTPNFEESSAYGKGILPPGQQWTGEVKSHGTHSEAGSLPAFTVPQAAALGNSTPSASSSSSCSTPSSPGDYYTSLDDNLKIEAIEKLFAMDTEAKDQCGTQTDFNELDLETLAPYIPMDGEDFQLSPICPEESLLPETPQSAPQHCFSTMSNIFQPLAPMASHSTFLLDKYQQQLESKKTEPEQRRVSFAFFDGGSRVSLLQCCGQTYTPLSSMGGISNTQWPPDPPLQLGPTKWPGEDRHAEAVGAAPLGLPPATPHLAMLKKRSAKGFGPQGPDVMSPAMIALSNKLKLKRQLEYEEQAFQDMSGGDPPGSGTSHLMWKRMKSLRGGGTCSLMPDKLPNANVPNDEFIQNPVRGRSQPLRHLSPPQPPSATSPGEPTKSGFPAQCYAPQYQDYSLPAAHKMSGMASRLLGPSFEPYLLPELTRYDCEVNVPVPGTSTLLQGGDLLRALDQAT

Target ORF information:

RefSeq Version	NM_174725.2
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelial PAS domain protein 1 (EPAS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174725.2

ORF Insert Sequence:

ATGACAGCTG ACAAGGAGAA GAAAAGGAGC AGCTCTGAGA GGAGGAAGGA GAAGTCCCGA 
GATGCAGCCA GGTGCCGGCG GAGCAAGGAA ACAGAGGTCT TCTATGAGCT GGCGCACGAG 
CTGCCCCTGC CGCACAGCGT GAGCTCGCAC CTGGACAAGG CCTCCATCAT GCGGCTGGCC 
ATCAGCTTCC TGCGCACGCA CAAGCTCCTG TCCTCTGTTT GCTCTGAAAA TGAATCTGAA 
GCCGACGCTG ACCAGCAGAT GGACAACTTG TATCTGAAAG CCTTGGAGGG TTTCATTGCC 
GTGGTGACCC AAGATGGTGA CATGATCTTT CTGTCGGAGA ACATCAGCAA GTTCATGGGA 
CTCACACAGG TAGAGTTAAC AGGACACAGT ATCTTTGACT TCACTCATCC CTGTGACCAT 
GAGGAGATCC GTGAGAACTT GAGCCTCAAA AATGGCTCTG GTTTTGGGAA GAAAAGCAAA 
GACATGTCCA CAGAGCGGGA CTTCTTCATG AGGATGAAGT GCACGGTCAC CAACAGAGGC 
CGCACAGTCA ACCTCAAGTC AGCCACCTGG AAGGTCTTGC ACTGCACGGG CCAGGTGAAG 
GTCTACAACA ACTGCCCCCC TCACAGCAGT CTCTGCGGCT GCAAGGAACC GCTGCTGTCC 
TGCCTCATCA TCATGTGTGA GCCGATCCAG CACCCATCCC ACATGGACAT CCCACTGGAC 
AGCAAGACCT TCCTGAGCCG CCACAGTATG GACATGAAGT TCACCTACTG CGACGACAGA 
ATCACAGAAC TGGTTGGTTA CCACCCTGAA GAGCTGCTTG GCCGCTCAGC CTATGAGTTC 
TACCATGCAC TGGACTCAGA GAACATGACC AAAAGCCACC AGAACTTGTG CACCAAGGGT 
CAGGTGGTGA GCGGCCAGTA CCGGATGCTT GCCAAGCACG GGGGCTATGT GTGGCTGGAG 
ACACAGGGGA CGGTGATCTA CAACCCTCGC AACCTGCAGC CCCAGTGCAT CATGTGTGTG 
AACTACGTCC TGAGTGAAAT TGAGAAGAAC GACGTGGTGT TCTCCATGGA TCAGACAGAG 
TCACTGTTTA AGCCGCACCT GCTGACCATG AACAGCATCT TTGATAACAG TGGCAAGGTG 
GCTGTGTCTG AGAAGAGCAA CTTCCTATTC ACCAAGCTGA AGGAGGAGCC CGAGGAGCTG 
GCCCAGCTGG CACCTACCGC GGGAGATACC ATCATTTCTC TGGATTTCGG GACCCCGAAC 
TTTGAGGAGT CCTCAGCCTA TGGCAAGGGC ATCCTGCCCC CAGGCCAGCA GTGGACCGGA 
GAGGTGAAGA GCCACGGCAC CCACAGTGAG GCCGGGAGCC TGCCCGCCTT CACTGTGCCC 
CAGGCGGCCG CCCTGGGGAA TAGCACCCCC AGTGCCAGCA GCAGCAGCAG CTGCTCCACG 
CCCAGTAGCC CTGGAGACTA TTACACATCT CTAGATGACA ATCTGAAGAT TGAAGCAATC 
GAGAAGCTCT TTGCCATGGA CACAGAGGCG AAGGACCAGT GTGGCACCCA GACTGACTTT 
AACGAGCTGG ATTTGGAGAC CCTGGCACCT TACATCCCCA TGGACGGAGA GGACTTCCAG 
CTTAGCCCCA TCTGCCCTGA GGAGAGCCTC CTGCCGGAGA CCCCCCAGTC GGCCCCCCAG 
CACTGCTTCA GCACCATGTC AAACATCTTC CAGCCACTGG CTCCGATGGC CTCTCACAGC 
ACCTTCCTCC TGGACAAGTA TCAGCAGCAG CTGGAAAGCA AGAAGACGGA GCCTGAGCAG 
CGGCGTGTGT CCTTCGCCTT CTTTGACGGT GGGAGCAGGG TGTCCCTGCT GCAGTGCTGT 
GGTCAGACCT ACACCCCCCT CTCCTCCATG GGGGGCATTT CCAACACCCA GTGGCCCCCT 
GACCCACCAC TACAGCTGGG GCCCACGAAG TGGCCTGGTG AAGACCGGCA CGCAGAGGCC 
GTGGGGGCAG CGCCCCTGGG GCTCCCCCCC GCCACACCCC ATCTCGCCAT GCTCAAGAAG 
AGGTCTGCCA AGGGCTTCGG GCCTCAGGGT CCAGACGTGA TGAGCCCAGC CATGATTGCC 
CTCTCCAACA AGCTGAAGCT GAAGCGACAG CTGGAGTACG AGGAGCAAGC CTTCCAAGAC 
ATGAGTGGGG GGGATCCGCC AGGCAGCGGC ACCTCGCATT TGATGTGGAA AAGAATGAAG 
AGTCTCAGGG GTGGTGGGAC CTGCTCTTTG ATGCCGGACA AGCTGCCAAA TGCAAATGTC 
CCTAATGATG AGTTCATCCA GAATCCCGTG AGGGGTAGGA GCCAGCCCCT GAGACACTTA 
TCACCACCAC AGCCTCCGTC TGCCACGAGC CCGGGGGAGC CCACCAAGAG TGGGTTCCCC 
GCTCAGTGTT ACGCCCCCCA GTACCAGGAC TACAGCCTAC CCGCGGCTCA CAAGATGTCA 
GGCATGGCAA GCCGGCTGCT CGGACCCTCG TTTGAGCCCT ACCTGCTGCC GGAACTGACC 
AGATATGACT GTGAGGTGAA CGTGCCTGTT CCGGGAACCT CCACGCTCCT CCAAGGAGGG 
GATCTCCTCC GAGCCCTGGA CCAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increased prevalence of EPAS1 variant in cattle with high-altitude pulmonary hypertension.
Nature communications66863(2015 Apr)
Newman JH,Holt TN,Cogan JD,Womack B,Phillips JA 3rd,Li C,Kendall Z,Stenmark KR,Thomas MG,Brown RD,Riddle SR,West JD,Hamid R

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

Molecular cloning of cDNAs encoding hypoxia-inducible factor (HIF)-1alpha and -2alpha of bovine arterial endothelial cells.
Biochimica et biophysica acta1445(2)237-43(1999 May)
Hara S,Kobayashi C,Imura N

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EPAS1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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