epas1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	epas1b
Entrez Gene ID	555192
Full Name	endothelial PAS domain protein 1b
Synonyms	epas1,wu:fq36f01
General protein information	Preferred Names endothelial PAS domain protein 1b Names endothelial PAS domain-containing protein 1 HIF2A endothelial PAS domain protein 1 hif-2a hypoxia inducible factor 2 alpha
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001039806.2	NP_001034895.2	endothelial PAS domain-containing protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EPAS1	XP_005626137.1
Rattus norvegicus (Norway rat)	Epas1	NP_075578.1
	EPAS1	XP_001112947.2
Mus musculus (house mouse)	Epas1	NP_034267.3
Pan troglodytes (chimpanzee)	EPAS1	XP_001147219.1
Gallus gallus (chicken)	EPAS1	NP_990138.1
Danio rerio (zebrafish)	epas1b	NP_001034895.1
Xenopus tropicalis (tropical clawed frog)	epas1	NP_001005647.1
Homo sapiens (human)	EPAS1	NP_001421.2
Bos taurus (cattle)	EPAS1	NP_777150.1

Gene Ontology
Bibliography

Related articles in PubMed

Hif-1? and Hif-2? regulate hemogenic endothelium and hematopoietic stem cell formation in zebrafish.
Gerri C, Marass M, Rossi A, Stainier DYR
Blood(2018 Jan)

Tet1 facilitates hypoxia tolerance by stabilizing the HIF-? proteins independent of its methylcytosine dioxygenase activity.
Wang J, Zhang D, Du J, Zhou C, Li Z, Liu X, Ouyang G, Xiao W
Nucleic acids research45(22)12700-12714(2017 Dec)

Regulation of the proprotein convertases expression and activity during regenerative angiogenesis: Role of hypoxia-inducible factor (HIF).
Ma J, Evrard S, Badiola I, Siegfried G, Khatib AM
European journal of cell biology96(5)457-468(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Tet1 modulates HIF-2alpha and HIF-1alpha through different mechanisms.
Title: Tet1 facilitates hypoxia tolerance by stabilizing the HIF-? proteins independent of its methylcytosine dioxygenase activity.

Hypoxic exposure (oxygen partial pressure = 5 kPa) in turn caused a significant increase in the level of Hif-1alpha protein even at 1 days post fertilization and in later stages, while neither Hif-2alpha nor Hif-3alpha protein level were affected.
Title: Developmental Expression and Hypoxic Induction of Hypoxia Inducible Transcription Factors in the Zebrafish.

hif2-alpha-leg1 axis in liver development may have resulted from the adaptation of teleosts to their environment
Title: Hypoxia-inducible factor 2 alpha is essential for hepatic outgrowth and functions via the regulation of leg1 transcription in the zebrafish embryo.

HIF-2alpha has a role in persistence of neutrophilic inflammation in humans, mice and zebrafish
Title: Hypoxia-inducible factor 2? regulates key neutrophil functions in humans, mice, and zebrafish.

Zebrafish HIF2alpha protects neural progenitor cells and neural differentiation processes by upregulating the survivin orthologues birc5a and birc5b during embryogenesis.
Title: Integration of CNS survival and differentiation by HIF2?.

The following epas1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the epas1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa42103	NM_001039806.2 Latest version!	Danio rerio endothelial PAS domain protein 1b (epas1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

epas1b ( NM_001039806.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001034895.2 Danio rerio endothelial PAS domain protein 1b (epas1b), mRNA.

Price & Availability↑

CloneID	ODa42103
Clone ID Related Accession (Same CDS sequence)	NM_001039806.2
Accession Version	NM_001039806.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-17
Organism	Danio rerio(zebrafish)
Product	endothelial PAS domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX248102.11. On Jun 16, 2015 this sequence version replaced NM_001039806.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ375242.1, GDQH01005427.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACAGCTG AGAAAGAGAA GAAGAGGTGC AGCTCAGAGC GGCGTAAGGA GAAATCTCGT 
GATGCCGCGC GCTGCAGACG GAGTAAAGAG ACAGAGGTGT TTTATGAACT GGCTCATCAT 
CTTCCCCTTC CACACAGCAT CAGCTCACAT TTGGATAAAG CGTCCATCAT GAGACTGGCT 
ATCAGCTTCC TGCGGACACG CAAACTCGTC AACTCAGGCT ACAATACTCC CACTGAAATG 
ACAGATGCAG ACAGACTCAT GGACAGTTGG TATCTGAAGT CACTCGGTGG CTTTATTACA 
GTGGTAACAT CAGATGGAGA CATGATCTTC TTATCGGAGA ACATCAACAA ATTCATGGGT 
CTCACTCAGG TGGAGCTAAC AGGACACAGC ATCTTCGACT TCACTCATCC CTGTGACCAT 
GAAGAGATCC GCGAGAACCT CAGCCTCAAA GCCGGCATTG GAAAGAAAGG CAAGGAGTTG 
AGCACAGAGA GGGATTTCTT CATGAGAATG AAATGCACAG TCACGAATCG AGGGCGCACC 
GTCAATCTTA AGTCAGCCAG CTGGAAGGTT CTACACTGCA CGGGTCATCT AAAGGTGTGT 
AACGGCTGCC CAGCACGAGT TCTGTGTGGT TTTAAGGAGC CGCCGCTCAC TTGTGTGGTC 
ATGATGTGCG AACCTATTGT CCATCCATCA AACATCGACA CACCGCTCGA CAGCAAGACA 
TTTCTGAGTC GCCACAGCAT GGACATGAAG TATACTTACT GTGATGAAAG GGTTACAGAG 
TTGATGGGCT ACAATCCTGA AGATCTTCTC GGACGATCTG CGTATGAGTT CTACCATGCT 
CTGGATGCTG AAAATGTCAC CAAAAGCCAC CAGAACCTGT GCACTAAAGG CCAGGCAGTG 
AGTGGTCAGT ACAGGATGTT GGCCAAGAAT GGGGGCTATG TGTGGGTGGA GACCCGGGGT 
ACCGTCATCT ACAACAGCCG CAACTCCCAG CCCCAGTGCA TTGTGTGCGT GAACTACGTG 
CTGAGTGACG TTGAGGAGAA ATCCTTGATC TTTTCCATGG ACCAGACTGA GTCTCTGTTC 
AAGCCTCACA AGTTGAATGG TTTCTTCAGT CCAAAAGAAG CCCTGGGCAG CGATCCAGCT 
GATTTGCTCT TCACCAAACT GAAAGAGGAG CCTGAAGATC TCACGCAGCT GGCACCTACA 
CCTGGGGACA CCATCATCTC TCTTGACTTT GGTCAGTCTC AGTATGAGGA GCACACAGTG 
TACAACAAAG TATCTTCAGT TGCACAGACT GTCTCTCACC CTGTTCATGA TGGCCACAGG 
ACAAGCTACT CTGGAGAAAT GGCCAAGATG GCCGCCACTT TCTCTGTGCC TCAATCTGCA 
CCTCCAAGTA GTGCAACTCC CAGCCTAAGC AGCTGCTCTA CGCCCAGCAG CCCAGATGAT 
TACTACACCC CTGTAGACAG TGACCTAAAG GTTGAATTGA CAGAGAAACT GTTTTCCTTG 
GACACGCAAG AGGCAAAGAC TTCCCGCAAC CAAGAGACTG ACTTGAGTGA TCTGGACTTG 
GAGACACTGG CTCCCTATAT CCCCATGGAT GGTGAGGATT TCCAGCTCAA TCCCATCTGT 
CCGGAAGAGC CGCCATCTGA GATTGGAACC CTGGGAACCA ACCAGCAGTG CTTCAGCAAC 
ATCACAAGCC TCTTCCAACC CTTGAGTTCA CCTTCAGCAG CTCATTACCA GCCCAAAATG 
AGTTCAGGAG GGGACAAGCA GAACATCAAT GGAGGCTCTG TGGAGTCATG GCCACCTGTT 
CCTTACAGCA GGGATCCCAT GCAGATGCCT CCATACCATG ATCCCGCCAG CACACCGCTG 
TCCTCAATGG GAGGGCGTCA GAATCTCCAA TGGCCGCCCG ACCCTCCTTT ACCCAGCAAG 
GCTGGAATGA TGGATCCTTT AGCTGCAAAG CGCTCTTGCC AAGGCATGCC AGCAAATCGA 
ATGGCACCTT TTATGCAAAG GCCTATGGAG AACTTTGTGC AGAACTACAG AGATACAAGT 
CCAGCTCGAC TTGCTCTTGC TAACAGTTTC AAACGCTCAT TCTCACAGAT GGCCATGGCA 
GAGACACCAC CTACTAAGTC TCAACAGACA GTGTGGAAGA AACTTCGGCA TGAGAGCTGT 
GCAGTCATGG AAAGGAAATC ACTCAGCAGT AGCGCATTGT CAGATAAAAG CATGGCCCAT 
AATGGAGGCA TGGACCACCA GCACAGGAAG AGCCAGTACT CGGGTAATCA GAACGGACAA 
CCGACAAAAC ACTACCGAGA ACAGTTCTGT AACTACAGAG AGTTCAACAT GCAGCCTTCC 
TCCAAAATGG ATGGAATCGC GAGCCGTCTT ATCGGGCCCT CCTTCGAGAC CTACTCACTG 
CCTGAACTGA CGCGTTACGA CTGCGAGGTC AATGTCCCGT TGCAAGGCAA TCTTCATTTG 
CTGCAGGGCA GTGACCTCCT AAGAGCATTA GACCAGGCGA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034895.2
CDS	293..2797
Translation	MTAEKEKKRCSSERRKEKSRDAARCRRSKETEVFYELAHHLPLPHSISSHLDKASIMRLAISFLRTRKLVNSGYNTPTEMTDADRLMDSWYLKSLGGFITVVTSDGDMIFLSENINKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKAGIGKKGKELSTERDFFMRMKCTVTNRGRTVNLKSASWKVLHCTGHLKVCNGCPARVLCGFKEPPLTCVVMMCEPIVHPSNIDTPLDSKTFLSRHSMDMKYTYCDERVTELMGYNPEDLLGRSAYEFYHALDAENVTKSHQNLCTKGQAVSGQYRMLAKNGGYVWVETRGTVIYNSRNSQPQCIVCVNYVLSDVEEKSLIFSMDQTESLFKPHKLNGFFSPKEALGSDPADLLFTKLKEEPEDLTQLAPTPGDTIISLDFGQSQYEEHTVYNKVSSVAQTVSHPVHDGHRTSYSGEMAKMAATFSVPQSAPPSSATPSLSSCSTPSSPDDYYTPVDSDLKVELTEKLFSLDTQEAKTSRNQETDLSDLDLETLAPYIPMDGEDFQLNPICPEEPPSEIGTLGTNQQCFSNITSLFQPLSSPSAAHYQPKMSSGGDKQNINGGSVESWPPVPYSRDPMQMPPYHDPASTPLSSMGGRQNLQWPPDPPLPSKAGMMDPLAAKRSCQGMPANRMAPFMQRPMENFVQNYRDTSPARLALANSFKRSFSQMAMAETPPTKSQQTVWKKLRHESCAVMERKSLSSSALSDKSMAHNGGMDHQHRKSQYSGNQNGQPTKHYREQFCNYREFNMQPSSKMDGIASRLIGPSFETYSLPELTRYDCEVNVPLQGNLHLLQGSDLLRALDQAT

Target ORF information:

RefSeq Version	NM_001039806.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio endothelial PAS domain protein 1b (epas1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039806.2

ORF Insert Sequence:

ATGACAGCTG AGAAAGAGAA GAAGAGGTGC AGCTCAGAGC GGCGTAAGGA GAAATCTCGT 
GATGCCGCGC GCTGCAGACG GAGTAAAGAG ACAGAGGTGT TTTATGAACT GGCTCATCAT 
CTTCCCCTTC CACACAGCAT CAGCTCACAT TTGGATAAAG CGTCCATCAT GAGACTGGCT 
ATCAGCTTCC TGCGGACACG CAAACTCGTC AACTCAGGCT ACAATACTCC CACTGAAATG 
ACAGATGCAG ACAGACTCAT GGACAGTTGG TATCTGAAGT CACTCGGTGG CTTTATTACA 
GTGGTAACAT CAGATGGAGA CATGATCTTC TTATCGGAGA ACATCAACAA ATTCATGGGT 
CTCACTCAGG TGGAGCTAAC AGGACACAGC ATCTTCGACT TCACTCATCC CTGTGACCAT 
GAAGAGATCC GCGAGAACCT CAGCCTCAAA GCCGGCATTG GAAAGAAAGG CAAGGAGTTG 
AGCACAGAGA GGGATTTCTT CATGAGAATG AAATGCACAG TCACGAATCG AGGGCGCACC 
GTCAATCTTA AGTCAGCCAG CTGGAAGGTT CTACACTGCA CGGGTCATCT AAAGGTGTGT 
AACGGCTGCC CAGCACGAGT TCTGTGTGGT TTTAAGGAGC CGCCGCTCAC TTGTGTGGTC 
ATGATGTGCG AACCTATTGT CCATCCATCA AACATCGACA CACCGCTCGA CAGCAAGACA 
TTTCTGAGTC GCCACAGCAT GGACATGAAG TATACTTACT GTGATGAAAG GGTTACAGAG 
TTGATGGGCT ACAATCCTGA AGATCTTCTC GGACGATCTG CGTATGAGTT CTACCATGCT 
CTGGATGCTG AAAATGTCAC CAAAAGCCAC CAGAACCTGT GCACTAAAGG CCAGGCAGTG 
AGTGGTCAGT ACAGGATGTT GGCCAAGAAT GGGGGCTATG TGTGGGTGGA GACCCGGGGT 
ACCGTCATCT ACAACAGCCG CAACTCCCAG CCCCAGTGCA TTGTGTGCGT GAACTACGTG 
CTGAGTGACG TTGAGGAGAA ATCCTTGATC TTTTCCATGG ACCAGACTGA GTCTCTGTTC 
AAGCCTCACA AGTTGAATGG TTTCTTCAGT CCAAAAGAAG CCCTGGGCAG CGATCCAGCT 
GATTTGCTCT TCACCAAACT GAAAGAGGAG CCTGAAGATC TCACGCAGCT GGCACCTACA 
CCTGGGGACA CCATCATCTC TCTTGACTTT GGTCAGTCTC AGTATGAGGA GCACACAGTG 
TACAACAAAG TATCTTCAGT TGCACAGACT GTCTCTCACC CTGTTCATGA TGGCCACAGG 
ACAAGCTACT CTGGAGAAAT GGCCAAGATG GCCGCCACTT TCTCTGTGCC TCAATCTGCA 
CCTCCAAGTA GTGCAACTCC CAGCCTAAGC AGCTGCTCTA CGCCCAGCAG CCCAGATGAT 
TACTACACCC CTGTAGACAG TGACCTAAAG GTTGAATTGA CAGAGAAACT GTTTTCCTTG 
GACACGCAAG AGGCAAAGAC TTCCCGCAAC CAAGAGACTG ACTTGAGTGA TCTGGACTTG 
GAGACACTGG CTCCCTATAT CCCCATGGAT GGTGAGGATT TCCAGCTCAA TCCCATCTGT 
CCGGAAGAGC CGCCATCTGA GATTGGAACC CTGGGAACCA ACCAGCAGTG CTTCAGCAAC 
ATCACAAGCC TCTTCCAACC CTTGAGTTCA CCTTCAGCAG CTCATTACCA GCCCAAAATG 
AGTTCAGGAG GGGACAAGCA GAACATCAAT GGAGGCTCTG TGGAGTCATG GCCACCTGTT 
CCTTACAGCA GGGATCCCAT GCAGATGCCT CCATACCATG ATCCCGCCAG CACACCGCTG 
TCCTCAATGG GAGGGCGTCA GAATCTCCAA TGGCCGCCCG ACCCTCCTTT ACCCAGCAAG 
GCTGGAATGA TGGATCCTTT AGCTGCAAAG CGCTCTTGCC AAGGCATGCC AGCAAATCGA 
ATGGCACCTT TTATGCAAAG GCCTATGGAG AACTTTGTGC AGAACTACAG AGATACAAGT 
CCAGCTCGAC TTGCTCTTGC TAACAGTTTC AAACGCTCAT TCTCACAGAT GGCCATGGCA 
GAGACACCAC CTACTAAGTC TCAACAGACA GTGTGGAAGA AACTTCGGCA TGAGAGCTGT 
GCAGTCATGG AAAGGAAATC ACTCAGCAGT AGCGCATTGT CAGATAAAAG CATGGCCCAT 
AATGGAGGCA TGGACCACCA GCACAGGAAG AGCCAGTACT CGGGTAATCA GAACGGACAA 
CCGACAAAAC ACTACCGAGA ACAGTTCTGT AACTACAGAG AGTTCAACAT GCAGCCTTCC 
TCCAAAATGG ATGGAATCGC GAGCCGTCTT ATCGGGCCCT CCTTCGAGAC CTACTCACTG 
CCTGAACTGA CGCGTTACGA CTGCGAGGTC AATGTCCCGT TGCAAGGCAA TCTTCATTTG 
CTGCAGGGCA GTGACCTCCT AAGAGCATTA GACCAGGCGA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hif-1? and Hif-2? regulate hemogenic endothelium and hematopoietic stem cell formation in zebrafish.
Blood(2018 Jan)
Gerri C,Marass M,Rossi A,Stainier DYR

Tet1 facilitates hypoxia tolerance by stabilizing the HIF-? proteins independent of its methylcytosine dioxygenase activity.
Nucleic acids research45(22)12700-12714(2017 Dec)
Wang J,Zhang D,Du J,Zhou C,Li Z,Liu X,Ouyang G,Xiao W

Regulation of the proprotein convertases expression and activity during regenerative angiogenesis: Role of hypoxia-inducible factor (HIF).
European journal of cell biology96(5)457-468(2017 Aug)
Ma J,Evrard S,Badiola I,Siegfried G,Khatib AM

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epas1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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