CAPN2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAPN2
Entrez Gene ID	281662
Full Name	calpain 2, (m/II) large subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103086.1	NP_001096556.1	calpain-2 catalytic subunit

Pathways from BioSystems

Homologies

	CAPN2	XP_001098172.1
Mus musculus (house mouse)	Capn2	NP_033924.2
Danio rerio (zebrafish)	zgc:92480	NP_001003485.1
Rattus norvegicus (Norway rat)	Capn2	NP_058812.1
Xenopus tropicalis (tropical clawed frog)	capn2.2	NP_001005446.1
Homo sapiens (human)	CAPN2	NP_001739.2
Pan troglodytes (chimpanzee)	CAPN2	XP_003308813.1
Danio rerio (zebrafish)	capn2l	NP_001017807.1
Canis lupus familiaris (dog)	CAPN2	XP_005622713.1
Bos taurus (cattle)	CAPN2	NP_001096556.1
Gallus gallus (chicken)	CAPN2	NP_990411.1

Gene Ontology
Bibliography

Related articles in PubMed

Association between IGF-IR, m-calpain and UCP-3 gene polymorphisms and growth traits in Nanyang cattle.
Zhang R, Li X
Molecular biology reports38(3)2179-84(2011 Mar)

Characteristic properties of endoplasmic reticulum membrane m-calpain, calpastatin and lumen m-calpain: a comparative study between membrane and lumen m-calpains.
Samanta K, Kar P, Chakraborti T, Shaikh S, Chakraborti S
Journal of biochemistry147(5)765-79(2010 May)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

m- and micro-calpain immunosignals in the cytoplasm both along the Z disk/I band regions and in the form of intracellular stores.
Title: Immunological detection of m- and ?-calpains in the skeletal muscle of Marchigiana cattle.

The objective of this study was to estimate the allele and genotype frequencies of the IGF-IR/TaqI, m-calpain/HhaI, and UCP-3/BglI polymorphisms and to determine association between these polymorphisms and growth traits in Chinese indigenous cattle breed.
Title: Association between IGF-IR, m-calpain and UCP-3 gene polymorphisms and growth traits in Nanyang cattle.

The cleavage of NCX1 by m-calpain in the caveolae vesicles may be interpreted as an important mechanism of Ca(2+) overload
Title: m-Calpain-mediated cleavage of Na+/Ca2+ exchanger-1 in caveolae vesicles isolated from pulmonary artery smooth muscle.

characteristic properties of ER membrane m-calpain (MCp), calpastatins and lumen m-calpain (LCp) and a brief comparative study between MCp and LCp
Title: Characteristic properties of endoplasmic reticulum membrane m-calpain, calpastatin and lumen m-calpain: a comparative study between membrane and lumen m-calpains.

The following CAPN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAPN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44172	NM_001103086.1 Latest version!	Bos taurus calpain 2 (CAPN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAPN2 ( NM_001103086.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001096556.1 Bos taurus calpain 2 (CAPN2), mRNA.

Price & Availability↑

CloneID	OBa44172
Clone ID Related Accession (Same CDS sequence)	NM_001103086.1
Accession Version	NM_001103086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	calpain-2 catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134526.1. On Sep 9, 2008 this sequence version replaced XM_864105.3. ##Evidence-Data-START## Transcript exon combination :: BC134526.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGCA TCGCGGCCAA GCTGGCGAAG GACCGGGAGG CGGCCGAGGG GCTGGGCTCG 
CACGAGAGGG CGGTCAAGTA CCTCAACCAG GACTACGCGG CGCTGCGGGA CGAGTGCCTG 
GAGGCCGGGG CGCTGTTCCA GGACCCCTCC TTCCCGGCCC TGCCGTCCTC CCTGGGCTTC 
AAGGAGTTGG GACCTTACTC CAGCAAGACC CGGGGCATCG AGTGGAAGCG GCCCACGGAG 
ATCTGCGATA ATCCCCAATT TATTACTGGA GGAGCCACTC GCACAGACAT CTGCCAAGGA 
GCCCTAGGTG ACTGCTGGCT GCTGGCCGCA ATTGCCTCCC TCACTTTGAA TGAAGAAATC 
CTGGCTCGAG TCGTCCCCCT GGACCAGAGC TTCCAGGAGA ACTATGCGGG GATCTTCCAC 
TTCCAGTTCT GGCAGTACGG CGAGTGGGTG GAGGTGGTGG TGGACGACAG GCTGCCCACC 
AAGGACGGGG AGCTGCTCTT CGTGCACTCG GCAGAGGGCA GCGAGTTCTG GAGTGCGCTG 
CTGGAGAAGG CCTACGCCAA GATCAATGGG TGTTATGAAG CACTTTCGGG GGGCGCCACC 
ACTGAGGGCT TCGAGGACTT CACTGGAGGC ATCGCTGAGT GGTATGAGTT GAGGAAGGCC 
CCTCCCAACC TGTTCAGGAT CATCCAGAAG GCTCTGCAGA AAGGCTCTCT CCTGGGCTGC 
TCCATCGATA TCACCAGCGC TGCGGACTCG GAGGCCATCA CGTTCCAGAA GCTGGTGAAG 
GGGCACGCGT ACTCGGTGAC CGGTGCCGAG GAGGTGGAAA GCAGAGGAAG CCTGCAGAAG 
CTGATCCGCA TCCGGAATCC CTGGGGAGAG GTGGAGTGGA CCGGGCAGTG GAATGACAAC 
TGCCCAAACT GGAACACAGT CGACCCCGAG GTGAGGGAAA CGCTGACTAG ACAGCATGAG 
GATGGGGAAT TCTGGATGTC TTTCAACGAC TTCCTGAGAC ACTATTCCCG CCTGGAAATC 
TGCAACCTGA CCCCTGACAC ACTCACCAGT GACAGCTACA AGAAGTGGAA ACTCACCAAG 
ATGGATGGGA ACTGGAGGCG GGGCTCCACC GCGGGCGGCT GCAGGAACTA CCCGAACACG 
TTCTGGATGA ATCCTCAGTA CCTGATCAAG CTGGAGGAAG AGGACGAGGA CCAGGAGGAC 
GGGGAGAGCG GCTGCACCTT CCTGGTGGGC CTCATCCAGA AGCACCGGCG GCGGCAGAGG 
AAGATGGGCG AGGACATGCA CACCATTGGC TTCGGCATCT ATGAGGTTCC AGAGGAGTTG 
ACTGGACAGA CCAACATCCA CCTCAGCAAA AAATTCTTCC TGACAACCAG AGCGCGGGAG 
CGGTCGGACA CCTTCATCAA CCTGCGGGAG GTGCTCAACC GCTTCAAGCT GCCCCCCGGG 
GAGTACATCG TGGTGCCCTC CACCTTCGAG CCCAACAAGG ACGGCGACTT CTGCATCCGG 
GTCTTTTCCG AGAAGAAAGC CGACTACCAA GTTGTCGACG ATGAAATCGA GGCCAACATT 
GACGAGATTG ACATCAGCGA GGATGACATC GATGATGGAT TCAGGAGACT GTTCGCCCAG 
CTGGCCGGAG AGGACGCAGA GATTTCTGCC TTTGAGTTGC AGACAATCCT GAGAAGAGTG 
CTAGCAAAGC GCCAAGATAT CAAGTCCGAC GGCTTTAGCA TTGAGACCTG CAAAATCATG 
GTGGACATGC TCGACTCGGA TGGGAGTGGC AAACTGGGTC TGAAGGAGTT CTACATTCTC 
TGGACGAAGA TTCAGAAATA CCAAAAAATT TACCGGGAAA TTGATGTGGA CCGGTCTGGA 
ACCATGAACT CCTATGAGAT GCGGAAAGCA TTAGAAGAAG CAGGTTTCAA GATGCCCTGT 
CAGCTCCACC AAGTGATTGT CGCCCGGTTT GCAGATGACG ACCTCATCAT CGATTTTGAC 
AATTTTGTCC GGTGTTTGAT TCGACTGGAG ACACTGTTCA GGATATTTAA GCAGCTGGAC 
CCCGAGAATA CTGGAATGAT CCAGCTTGAC CTTATCTCTT GGCTGTCTTT TTCAGTACTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096556.1
CDS	134..2236
Translation	MAGIAAKLAKDREAAEGLGSHERAVKYLNQDYAALRDECLEAGALFQDPSFPALPSSLGFKELGPYSSKTRGIEWKRPTEICDNPQFITGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLDQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELRKAPPNLFRIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESRGSLQKLIRIRNPWGEVEWTGQWNDNCPNWNTVDPEVRETLTRQHEDGEFWMSFNDFLRHYSRLEICNLTPDTLTSDSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDQEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELTGQTNIHLSKKFFLTTRARERSDTFINLREVLNRFKLPPGEYIVVPSTFEPNKDGDFCIRVFSEKKADYQVVDDEIEANIDEIDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDDLIIDFDNFVRCLIRLETLFRIFKQLDPENTGMIQLDLISWLSFSVL

Target ORF information:

RefSeq Version	NM_001103086.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calpain 2 (CAPN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103086.1

ORF Insert Sequence:

ATGGCGGGCA TCGCGGCCAA GCTGGCGAAG GACCGGGAGG CGGCCGAGGG GCTGGGCTCG 
CACGAGAGGG CGGTCAAGTA CCTCAACCAG GACTACGCGG CGCTGCGGGA CGAGTGCCTG 
GAGGCCGGGG CGCTGTTCCA GGACCCCTCC TTCCCGGCCC TGCCGTCCTC CCTGGGCTTC 
AAGGAGTTGG GACCTTACTC CAGCAAGACC CGGGGCATCG AGTGGAAGCG GCCCACGGAG 
ATCTGCGATA ATCCCCAATT TATTACTGGA GGAGCCACTC GCACAGACAT CTGCCAAGGA 
GCCCTAGGTG ACTGCTGGCT GCTGGCCGCA ATTGCCTCCC TCACTTTGAA TGAAGAAATC 
CTGGCTCGAG TCGTCCCCCT GGACCAGAGC TTCCAGGAGA ACTATGCGGG GATCTTCCAC 
TTCCAGTTCT GGCAGTACGG CGAGTGGGTG GAGGTGGTGG TGGACGACAG GCTGCCCACC 
AAGGACGGGG AGCTGCTCTT CGTGCACTCG GCAGAGGGCA GCGAGTTCTG GAGTGCGCTG 
CTGGAGAAGG CCTACGCCAA GATCAATGGG TGTTATGAAG CACTTTCGGG GGGCGCCACC 
ACTGAGGGCT TCGAGGACTT CACTGGAGGC ATCGCTGAGT GGTATGAGTT GAGGAAGGCC 
CCTCCCAACC TGTTCAGGAT CATCCAGAAG GCTCTGCAGA AAGGCTCTCT CCTGGGCTGC 
TCCATCGATA TCACCAGCGC TGCGGACTCG GAGGCCATCA CGTTCCAGAA GCTGGTGAAG 
GGGCACGCGT ACTCGGTGAC CGGTGCCGAG GAGGTGGAAA GCAGAGGAAG CCTGCAGAAG 
CTGATCCGCA TCCGGAATCC CTGGGGAGAG GTGGAGTGGA CCGGGCAGTG GAATGACAAC 
TGCCCAAACT GGAACACAGT CGACCCCGAG GTGAGGGAAA CGCTGACTAG ACAGCATGAG 
GATGGGGAAT TCTGGATGTC TTTCAACGAC TTCCTGAGAC ACTATTCCCG CCTGGAAATC 
TGCAACCTGA CCCCTGACAC ACTCACCAGT GACAGCTACA AGAAGTGGAA ACTCACCAAG 
ATGGATGGGA ACTGGAGGCG GGGCTCCACC GCGGGCGGCT GCAGGAACTA CCCGAACACG 
TTCTGGATGA ATCCTCAGTA CCTGATCAAG CTGGAGGAAG AGGACGAGGA CCAGGAGGAC 
GGGGAGAGCG GCTGCACCTT CCTGGTGGGC CTCATCCAGA AGCACCGGCG GCGGCAGAGG 
AAGATGGGCG AGGACATGCA CACCATTGGC TTCGGCATCT ATGAGGTTCC AGAGGAGTTG 
ACTGGACAGA CCAACATCCA CCTCAGCAAA AAATTCTTCC TGACAACCAG AGCGCGGGAG 
CGGTCGGACA CCTTCATCAA CCTGCGGGAG GTGCTCAACC GCTTCAAGCT GCCCCCCGGG 
GAGTACATCG TGGTGCCCTC CACCTTCGAG CCCAACAAGG ACGGCGACTT CTGCATCCGG 
GTCTTTTCCG AGAAGAAAGC CGACTACCAA GTTGTCGACG ATGAAATCGA GGCCAACATT 
GACGAGATTG ACATCAGCGA GGATGACATC GATGATGGAT TCAGGAGACT GTTCGCCCAG 
CTGGCCGGAG AGGACGCAGA GATTTCTGCC TTTGAGTTGC AGACAATCCT GAGAAGAGTG 
CTAGCAAAGC GCCAAGATAT CAAGTCCGAC GGCTTTAGCA TTGAGACCTG CAAAATCATG 
GTGGACATGC TCGACTCGGA TGGGAGTGGC AAACTGGGTC TGAAGGAGTT CTACATTCTC 
TGGACGAAGA TTCAGAAATA CCAAAAAATT TACCGGGAAA TTGATGTGGA CCGGTCTGGA 
ACCATGAACT CCTATGAGAT GCGGAAAGCA TTAGAAGAAG CAGGTTTCAA GATGCCCTGT 
CAGCTCCACC AAGTGATTGT CGCCCGGTTT GCAGATGACG ACCTCATCAT CGATTTTGAC 
AATTTTGTCC GGTGTTTGAT TCGACTGGAG ACACTGTTCA GGATATTTAA GCAGCTGGAC 
CCCGAGAATA CTGGAATGAT CCAGCTTGAC CTTATCTCTT GGCTGTCTTT TTCAGTACTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association between IGF-IR, m-calpain and UCP-3 gene polymorphisms and growth traits in Nanyang cattle.
Molecular biology reports38(3)2179-84(2011 Mar)
Zhang R,Li X

Characteristic properties of endoplasmic reticulum membrane m-calpain, calpastatin and lumen m-calpain: a comparative study between membrane and lumen m-calpains.
Journal of biochemistry147(5)765-79(2010 May)
Samanta K,Kar P,Chakraborti T,Shaikh S,Chakraborti S

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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CAPN2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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