capn2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	capn2
Entrez Gene ID	448035
Full Name	calpain 2, gene2
Synonyms	canp2,canpl2,canpml,capn2,mcanp
General protein information	Preferred Names calpain 2, gene2 Names calpain-2 catalytic subunit calpain 2, (m/II) large subunit
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031901618.1	XP_031757478.1	calpain-2 catalytic subunit isoform X3
NM_001005446.1	NP_001005446.1	calpain-2 catalytic subunit
XM_031901616.1	XP_031757476.1	calpain-2 catalytic subunit isoform X1
XM_031901617.1	XP_031757477.1	calpain-2 catalytic subunit isoform X2

Pathways from BioSystems

Homologies

	CAPN2	XP_001098172.1
Mus musculus (house mouse)	Capn2	NP_033924.2
Danio rerio (zebrafish)	zgc:92480	NP_001003485.1
Rattus norvegicus (Norway rat)	Capn2	NP_058812.1
Xenopus tropicalis (tropical clawed frog)	capn2.2	NP_001005446.1
Homo sapiens (human)	CAPN2	NP_001739.2
Pan troglodytes (chimpanzee)	CAPN2	XP_003308813.1
Danio rerio (zebrafish)	capn2l	NP_001017807.1
Canis lupus familiaris (dog)	CAPN2	XP_005622713.1
Bos taurus (cattle)	CAPN2	NP_001096556.1
Gallus gallus (chicken)	CAPN2	NP_990411.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following capn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the capn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa01801	NM_001005446.1 Latest version!	Xenopus tropicalis calpain 2 (capn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa57967	XM_031901616.1 Latest version!	Xenopus tropicalis calpain 2 (capn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa57968	XM_031901617.1 Latest version!	Xenopus tropicalis calpain 2 (capn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa57969	XM_031901618.1 Latest version!	Xenopus tropicalis calpain 2 (capn2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

capn2 ( NM_001005446.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001005446.1 Xenopus tropicalis calpain 2 (capn2), mRNA.

Price & Availability↑

CloneID	OXa01801
Clone ID Related Accession (Same CDS sequence)	NM_001005446.1
Accession Version	NM_001005446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpain-2 catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC074555.1. ##Evidence-Data-START## Transcript exon combination :: BC074555.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGAGCGGGG TGGCTGATAG GCTGGCCAAG GAGCGGGCAC TGGCAAGTGG CCTTGGCACC 
AATGAGAAGG CGCTGCCCTA CCTGAGCCAG GACTATGAGG CGCTGCGCAA TGAGTGTCTC 
CAGTCTGGGA CCCTGTTTAA GGACCTCAGC TTCCCGGCCA TCCACTCCTC CCTGGGGTTC 
AAAGAGCTGG GGCAGCATTC GGCCAAATGC CAAGGGGTGG TATGGAAGAG ACCCAAGGAC 
CTGTGCCCAG ACCCTCAGTT CATCGCAGGA GGAGCGACTC GCACTGATAT CTGCCAAGGA 
GCCCTGGGTG ACTGCTGGTT ACTTGCGGCC ATTGCCTCCC TCACCCTGAA TGAGCAGATT 
CTGTCCCGGG TTGTTCCAAA GGATCAGAGC TTCCAAGAGA ACTATGCCGG AATATTCCAC 
TTCCAGTTCT GGCAGTACGG TGAGTGGGTG GATGTTGTCA TTGATGATCG TCTGCCAACC 
AAGGATGGGA AGCTGCTTTT TGTGCATTCA GCGGAGGGGA GTGAGTTCTG GAGCGCACTC 
ATGGAGAAGG CTTATGCCAA ACTGAACGGA TCCTATGAAG CTCTCTCGGG TGGGACCACC 
ACCGAAGGGT TTGAAGATTT CACAGGTGGA CTTGCAGAGT GGTATGAACT GAGGAAGGCC 
CCACCCAATC TGTTCAAGAT TATCCAGAAA GCTCTGAGGA CTGGATCTCT GCTGGGCTGC 
TCTATAGATA TCACAAGTGC AGCGGAGACA GAAGCCATCA CATTCCAGAA GCTCGTGAAG 
GGCCATGCCT ACTCTGTGAC TGCAGCAGAA GAGGTGCAAT ATCGAGGGAA CCTGGAAAAG 
CTGATCAGAA TCAGAAATCC CTGGGGGGAA GTGGAATGGA CCGGGGCCTG GAGTGACAAT 
TCCAGCGAGT GGAATTCTGT GGATCCGGAT GTGCGGGAAA AGTTGATCAA GAGATGTGAC 
GATGGAGAGT TTTGGATGTC CTTCAATGAC TTCTTGAGAA ACTATTCAAG ACTGGAGATC 
TGCAATCTGA CCCCGGACAC TCTGGCCTCT GACAAATACA GTAAGTGGAG TCTGACCAAG 
ATGGACGGCA CGTGGAGGAG AGGCTCGACA GCCGGCGGAT GCAGGAATTT CCCAGAAACC 
TTCTGGATGA ACCCCCAGTA TGTGATTAAG CTGGAAGAAG CAGATGATGA TCCTGATGAT 
AATGAGGAAG GCTGCACCTT TATTGTCGGT CTGATACAGA AGAACCGCAG GAAGCAGAGG 
AAGATGGGAG AGGACATGCA CACCATTGGC TTTGCCATCT ATGAGGTGCC GCCACAGCTC 
ACTGGCCAGA AGGACATTCG CCTGAGCCGA GATTACATTC TAAGGAACAA GATTCGGGAA 
AAATCCGACA CCTATATAAA CCTGCGCGAG GTGCTGAACA GATTCCGGCT GCCCCCAGGG 
GAGTACGTCA TTCTGCCTTC CACATTCGAA CCCCACAAAG TCGGAGACTT CTGCCTGCGG 
GTCTTCTCTG AAAAGAACGC CGAGTCCAAA GTTCCTGATG ATGAAATCAA GGCTGACATT 
GATGAGGACG TCAATGAAGA TGCCATCGAC CCCAACTTCA AAAGGATGTT CCTGGCGCTG 
GCAGGAGATG ACAAAGAGAT CTCTCCGCAT GAACTCTTTA ACATCCTACA GAAGGTGATA 
TCCAAGCGAG AAGACATAAA GTCCGATGGG TTCAGCATAG AGACCTGTAG GACAATTGTT 
GACCTGCTGG ATTCTGATGG TACAGGGAAA CTTGGCCTTA AGGAGTTCAA AATCCTATGG 
ACAAAAATAC TGAAGTACCA GAAAATATAC AGCAGCGTGG ATAGGGACCA CTCCGGCACT 
ATTAATTCCT ATGAGATGCG GGGAGCCCTG GAAGCTGCAG GGATTAAGGT GAACTCCACC 
ATTAATGAGC TGCTGGTGGC TCGCTTCGCG GATGAAGACC ATACCATTGA CTTTGATAAC 
TTTGTGAGAT GCCTTCTGCG CCTGGAGATC ATGTTCAAGA TCTTCAACCA GATGGACACT 
GAGAAGACCG GAGTGGTGAC TCTGAAGATG GACACTTGGC TTTCCCTGAC TGTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005446.1
CDS	79..2178
Translation	MSGVADRLAKERALASGLGTNEKALPYLSQDYEALRNECLQSGTLFKDLSFPAIHSSLGFKELGQHSAKCQGVVWKRPKDLCPDPQFIAGGATRTDICQGALGDCWLLAAIASLTLNEQILSRVVPKDQSFQENYAGIFHFQFWQYGEWVDVVIDDRLPTKDGKLLFVHSAEGSEFWSALMEKAYAKLNGSYEALSGGTTTEGFEDFTGGLAEWYELRKAPPNLFKIIQKALRTGSLLGCSIDITSAAETEAITFQKLVKGHAYSVTAAEEVQYRGNLEKLIRIRNPWGEVEWTGAWSDNSSEWNSVDPDVREKLIKRCDDGEFWMSFNDFLRNYSRLEICNLTPDTLASDKYSKWSLTKMDGTWRRGSTAGGCRNFPETFWMNPQYVIKLEEADDDPDDNEEGCTFIVGLIQKNRRKQRKMGEDMHTIGFAIYEVPPQLTGQKDIRLSRDYILRNKIREKSDTYINLREVLNRFRLPPGEYVILPSTFEPHKVGDFCLRVFSEKNAESKVPDDEIKADIDEDVNEDAIDPNFKRMFLALAGDDKEISPHELFNILQKVISKREDIKSDGFSIETCRTIVDLLDSDGTGKLGLKEFKILWTKILKYQKIYSSVDRDHSGTINSYEMRGALEAAGIKVNSTINELLVARFADEDHTIDFDNFVRCLLRLEIMFKIFNQMDTEKTGVVTLKMDTWLSLTVI

Target ORF information:

RefSeq Version	NM_001005446.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpain 2 (capn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005446.1

ORF Insert Sequence:

ATGAGCGGGG TGGCTGATAG GCTGGCCAAG GAGCGGGCAC TGGCAAGTGG CCTTGGCACC 
AATGAGAAGG CGCTGCCCTA CCTGAGCCAG GACTATGAGG CGCTGCGCAA TGAGTGTCTC 
CAGTCTGGGA CCCTGTTTAA GGACCTCAGC TTCCCGGCCA TCCACTCCTC CCTGGGGTTC 
AAAGAGCTGG GGCAGCATTC GGCCAAATGC CAAGGGGTGG TATGGAAGAG ACCCAAGGAC 
CTGTGCCCAG ACCCTCAGTT CATCGCAGGA GGAGCGACTC GCACTGATAT CTGCCAAGGA 
GCCCTGGGTG ACTGCTGGTT ACTTGCGGCC ATTGCCTCCC TCACCCTGAA TGAGCAGATT 
CTGTCCCGGG TTGTTCCAAA GGATCAGAGC TTCCAAGAGA ACTATGCCGG AATATTCCAC 
TTCCAGTTCT GGCAGTACGG TGAGTGGGTG GATGTTGTCA TTGATGATCG TCTGCCAACC 
AAGGATGGGA AGCTGCTTTT TGTGCATTCA GCGGAGGGGA GTGAGTTCTG GAGCGCACTC 
ATGGAGAAGG CTTATGCCAA ACTGAACGGA TCCTATGAAG CTCTCTCGGG TGGGACCACC 
ACCGAAGGGT TTGAAGATTT CACAGGTGGA CTTGCAGAGT GGTATGAACT GAGGAAGGCC 
CCACCCAATC TGTTCAAGAT TATCCAGAAA GCTCTGAGGA CTGGATCTCT GCTGGGCTGC 
TCTATAGATA TCACAAGTGC AGCGGAGACA GAAGCCATCA CATTCCAGAA GCTCGTGAAG 
GGCCATGCCT ACTCTGTGAC TGCAGCAGAA GAGGTGCAAT ATCGAGGGAA CCTGGAAAAG 
CTGATCAGAA TCAGAAATCC CTGGGGGGAA GTGGAATGGA CCGGGGCCTG GAGTGACAAT 
TCCAGCGAGT GGAATTCTGT GGATCCGGAT GTGCGGGAAA AGTTGATCAA GAGATGTGAC 
GATGGAGAGT TTTGGATGTC CTTCAATGAC TTCTTGAGAA ACTATTCAAG ACTGGAGATC 
TGCAATCTGA CCCCGGACAC TCTGGCCTCT GACAAATACA GTAAGTGGAG TCTGACCAAG 
ATGGACGGCA CGTGGAGGAG AGGCTCGACA GCCGGCGGAT GCAGGAATTT CCCAGAAACC 
TTCTGGATGA ACCCCCAGTA TGTGATTAAG CTGGAAGAAG CAGATGATGA TCCTGATGAT 
AATGAGGAAG GCTGCACCTT TATTGTCGGT CTGATACAGA AGAACCGCAG GAAGCAGAGG 
AAGATGGGAG AGGACATGCA CACCATTGGC TTTGCCATCT ATGAGGTGCC GCCACAGCTC 
ACTGGCCAGA AGGACATTCG CCTGAGCCGA GATTACATTC TAAGGAACAA GATTCGGGAA 
AAATCCGACA CCTATATAAA CCTGCGCGAG GTGCTGAACA GATTCCGGCT GCCCCCAGGG 
GAGTACGTCA TTCTGCCTTC CACATTCGAA CCCCACAAAG TCGGAGACTT CTGCCTGCGG 
GTCTTCTCTG AAAAGAACGC CGAGTCCAAA GTTCCTGATG ATGAAATCAA GGCTGACATT 
GATGAGGACG TCAATGAAGA TGCCATCGAC CCCAACTTCA AAAGGATGTT CCTGGCGCTG 
GCAGGAGATG ACAAAGAGAT CTCTCCGCAT GAACTCTTTA ACATCCTACA GAAGGTGATA 
TCCAAGCGAG AAGACATAAA GTCCGATGGG TTCAGCATAG AGACCTGTAG GACAATTGTT 
GACCTGCTGG ATTCTGATGG TACAGGGAAA CTTGGCCTTA AGGAGTTCAA AATCCTATGG 
ACAAAAATAC TGAAGTACCA GAAAATATAC AGCAGCGTGG ATAGGGACCA CTCCGGCACT 
ATTAATTCCT ATGAGATGCG GGGAGCCCTG GAAGCTGCAG GGATTAAGGT GAACTCCACC 
ATTAATGAGC TGCTGGTGGC TCGCTTCGCG GATGAAGACC ATACCATTGA CTTTGATAAC 
TTTGTGAGAT GCCTTCTGCG CCTGGAGATC ATGTTCAAGA TCTTCAACCA GATGGACACT 
GAGAAGACCG GAGTGGTGAC TCTGAAGATG GACACTTGGC TTTCCCTGAC TGTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn2 ( XM_031901616.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757476.1 Xenopus tropicalis calpain 2 (capn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa57967
Clone ID Related Accession (Same CDS sequence)	XM_031901616.1
Accession Version	XM_031901616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpain-2 catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGGG TGGCTGATAG GCTGGCCAAG GAGCGGGCAC TGGCAAGTGG CCTTGGCACC 
AATGAGAAGG CGCTGCCCTA CCTGAGCCAG GACTATGAGG CGCTGCGCAA TGAGTGTCTC 
CAGTCTGGGA CCCTGTTTAA GGACCTCAGC TTCCCGGCCA TCCACTCCTC CCTGGGGTTC 
AAAGAGCTGG GGCAGCATTC GGCCAAATGC CAAGGGGTGG TATGGAAGAG ACCCAAGGAC 
CTGTGCCCAG ACCCTCAGTT CATCGCAGGA GGAGCGACTC GCACTGATAT CTGCCAAGGA 
GCCCTGGGTG ACTGCTGGTT ACTTGCGGCC ATTGCCTCCC TCACCCTGAA TGAGCAGATT 
CTGTCCCGGG TTGTTCCAAA GGATCAGAGC TTCCAAGAGA ACTATGCCGG AATATTCCAC 
TTCCAGTTCT GGCAGTACGG TGAGTGGGTG GATGTTGTCA TTGATGATCG TCTGCCAACC 
AAGGATGGGA AGCTGCTTTT TGTGCATTCA GCGGAGGGGA GTGAGTTCTG GAGCGCACTC 
ATGGAGAAGG CTTATGCCAA ACTGAACGGA TCCTATGAAG CTCTCTCGGG TGGGACCACC 
ACCGAAGGGT TTGAAGATTT CACAGGTGGA CTTGCAGAGT GGTATGAACT GAGGAAGGCC 
CCACCCAATC TGTTCAAGAT TATCCAGAAA GCTCTGAGGA CTGGATCTCT GCTGGGCTGC 
TCTATAGATA TCACAAGTGC AGCGGAGACA GAAGCCATCA CATTCCAGAA GCTCGTGAAG 
GGCCATGCCT ACTCTGTGAC TGCAGCAGAA GAGGTGCAAT ATCGAGGGAA CCTGGAAAAG 
CTGATCAGAA TCAGAAATCC CTGGGGGGAA GTGGAATGGA CCGGGGCCTG GAGTGACAAT 
TCCAGCGAGT GGAATTCTGT GGATCCGGAT GTGCGGGAAA AGTTGATCAA GAGATGTGAC 
GATGGAGAGT TTTGGATGTC CTTCAATGAC TTCTTGAGAA ACTATTCAAG ACTGGAGATC 
TGCAATCTGA CCCCGGACAC TCTGGCCTCT GACAAATACA GTAAGTGGAG TCTGACCAAG 
ATGGACGGCA CGTGGAGGAG AGGCTCGACA GCCGGCGGAT GCAGGAATTT CCCAGAAACC 
TTCTGGATGA ACCCCCAGTA TGTGATTAAG CTGGAAGAAG CAGATGATGA TCCTGATGAT 
AATGAGGAAG GCTGCACCTT TATTGTCGGT CTGATACAGA AGAACCGCAG GAAGCAGAGG 
AAGATGGGAG AGGACATGCA CACCATTGGC TTTGCCATCT ATGAGGTGCC GCCACAGCTC 
ACTGGCCAGA AGGACATTCG CCTGAGCCGA GATTACATTC TAAGGAACAA GATTCGGGAA 
AAATCCGACA CCTATATAAA CCTGCGCGAG GTGCTGAACA GATTCCGGCT GCCCCCAGGG 
GAGTACGTCA TTCTGCCTTC CACATTCGAA CCCCACAAAG TCGGAGACTT CTGCCTGCGG 
GTCTTCTCTG AAAAGAACGC CGAGTCCAAG TGCCAAGACA GAGTTCCTGA TGATGAAATC 
AAGGCTGACA TTGATGAGGA CGTCAATGAA GATGCCATCG ACCCCAACTT CAAAAGGATG 
TTCCTGGCGC TGGCAGGAGA TGACAAAGAG ATCTCTCCGC ATGAACTCTT TAACATCCTA 
CAGAAGGTGA TATCCAAGCG AGAAGACATA AAGTCCGATG GGTTCAGCAT AGAGACCTGT 
AGGACAATTG TTGACCTGCT GGATTCTGAT GGTACAGGGA AACTTGGCCT TAAGGAGTTC 
AAAATCCTAT GGACAAAAAT ACTGAAGTAC CAGAAAATAT ACAGCAGCGT GGATAGGGAC 
CACTCCGGCA CTATTAATTC CTATGAGATG CGGGGAGCCC TGGAAGCTGC AGGGATTAAG 
GTGAACTCCA CCATTAATGA GCTGCTGGTG GCTCGCTTCG CGGATGAAGA CCATACCATT 
GACTTTGATA ACTTTGTGAG ATGCCTTCTG CGCCTGGAGA TCATGTTCAA GATCTTCAAC 
CAGATGGACA CTGAGAAGAC CGGAGTGGTG ACTCTGAAGA TGGACACTTG GCTTTCCCTG 
ACTGTGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757476.1
CDS	216..2327
Translation	MSGVADRLAKERALASGLGTNEKALPYLSQDYEALRNECLQSGTLFKDLSFPAIHSSLGFKELGQHSAKCQGVVWKRPKDLCPDPQFIAGGATRTDICQGALGDCWLLAAIASLTLNEQILSRVVPKDQSFQENYAGIFHFQFWQYGEWVDVVIDDRLPTKDGKLLFVHSAEGSEFWSALMEKAYAKLNGSYEALSGGTTTEGFEDFTGGLAEWYELRKAPPNLFKIIQKALRTGSLLGCSIDITSAAETEAITFQKLVKGHAYSVTAAEEVQYRGNLEKLIRIRNPWGEVEWTGAWSDNSSEWNSVDPDVREKLIKRCDDGEFWMSFNDFLRNYSRLEICNLTPDTLASDKYSKWSLTKMDGTWRRGSTAGGCRNFPETFWMNPQYVIKLEEADDDPDDNEEGCTFIVGLIQKNRRKQRKMGEDMHTIGFAIYEVPPQLTGQKDIRLSRDYILRNKIREKSDTYINLREVLNRFRLPPGEYVILPSTFEPHKVGDFCLRVFSEKNAESKCQDRVPDDEIKADIDEDVNEDAIDPNFKRMFLALAGDDKEISPHELFNILQKVISKREDIKSDGFSIETCRTIVDLLDSDGTGKLGLKEFKILWTKILKYQKIYSSVDRDHSGTINSYEMRGALEAAGIKVNSTINELLVARFADEDHTIDFDNFVRCLLRLEIMFKIFNQMDTEKTGVVTLKMDTWLSLTVI

Target ORF information:

RefSeq Version	XM_031901616.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpain 2 (capn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901616.1

ORF Insert Sequence:

ATGAGCGGGG TGGCTGATAG GCTGGCCAAG GAGCGGGCAC TGGCAAGTGG CCTTGGCACC 
AATGAGAAGG CGCTGCCCTA CCTGAGCCAG GACTATGAGG CGCTGCGCAA TGAGTGTCTC 
CAGTCTGGGA CCCTGTTTAA GGACCTCAGC TTCCCGGCCA TCCACTCCTC CCTGGGGTTC 
AAAGAGCTGG GGCAGCATTC GGCCAAATGC CAAGGGGTGG TATGGAAGAG ACCCAAGGAC 
CTGTGCCCAG ACCCTCAGTT CATCGCAGGA GGAGCGACTC GCACTGATAT CTGCCAAGGA 
GCCCTGGGTG ACTGCTGGTT ACTTGCGGCC ATTGCCTCCC TCACCCTGAA TGAGCAGATT 
CTGTCCCGGG TTGTTCCAAA GGATCAGAGC TTCCAAGAGA ACTATGCCGG AATATTCCAC 
TTCCAGTTCT GGCAGTACGG TGAGTGGGTG GATGTTGTCA TTGATGATCG TCTGCCAACC 
AAGGATGGGA AGCTGCTTTT TGTGCATTCA GCGGAGGGGA GTGAGTTCTG GAGCGCACTC 
ATGGAGAAGG CTTATGCCAA ACTGAACGGA TCCTATGAAG CTCTCTCGGG TGGGACCACC 
ACCGAAGGGT TTGAAGATTT CACAGGTGGA CTTGCAGAGT GGTATGAACT GAGGAAGGCC 
CCACCCAATC TGTTCAAGAT TATCCAGAAA GCTCTGAGGA CTGGATCTCT GCTGGGCTGC 
TCTATAGATA TCACAAGTGC AGCGGAGACA GAAGCCATCA CATTCCAGAA GCTCGTGAAG 
GGCCATGCCT ACTCTGTGAC TGCAGCAGAA GAGGTGCAAT ATCGAGGGAA CCTGGAAAAG 
CTGATCAGAA TCAGAAATCC CTGGGGGGAA GTGGAATGGA CCGGGGCCTG GAGTGACAAT 
TCCAGCGAGT GGAATTCTGT GGATCCGGAT GTGCGGGAAA AGTTGATCAA GAGATGTGAC 
GATGGAGAGT TTTGGATGTC CTTCAATGAC TTCTTGAGAA ACTATTCAAG ACTGGAGATC 
TGCAATCTGA CCCCGGACAC TCTGGCCTCT GACAAATACA GTAAGTGGAG TCTGACCAAG 
ATGGACGGCA CGTGGAGGAG AGGCTCGACA GCCGGCGGAT GCAGGAATTT CCCAGAAACC 
TTCTGGATGA ACCCCCAGTA TGTGATTAAG CTGGAAGAAG CAGATGATGA TCCTGATGAT 
AATGAGGAAG GCTGCACCTT TATTGTCGGT CTGATACAGA AGAACCGCAG GAAGCAGAGG 
AAGATGGGAG AGGACATGCA CACCATTGGC TTTGCCATCT ATGAGGTGCC GCCACAGCTC 
ACTGGCCAGA AGGACATTCG CCTGAGCCGA GATTACATTC TAAGGAACAA GATTCGGGAA 
AAATCCGACA CCTATATAAA CCTGCGCGAG GTGCTGAACA GATTCCGGCT GCCCCCAGGG 
GAGTACGTCA TTCTGCCTTC CACATTCGAA CCCCACAAAG TCGGAGACTT CTGCCTGCGG 
GTCTTCTCTG AAAAGAACGC CGAGTCCAAG TGCCAAGACA GAGTTCCTGA TGATGAAATC 
AAGGCTGACA TTGATGAGGA CGTCAATGAA GATGCCATCG ACCCCAACTT CAAAAGGATG 
TTCCTGGCGC TGGCAGGAGA TGACAAAGAG ATCTCTCCGC ATGAACTCTT TAACATCCTA 
CAGAAGGTGA TATCCAAGCG AGAAGACATA AAGTCCGATG GGTTCAGCAT AGAGACCTGT 
AGGACAATTG TTGACCTGCT GGATTCTGAT GGTACAGGGA AACTTGGCCT TAAGGAGTTC 
AAAATCCTAT GGACAAAAAT ACTGAAGTAC CAGAAAATAT ACAGCAGCGT GGATAGGGAC 
CACTCCGGCA CTATTAATTC CTATGAGATG CGGGGAGCCC TGGAAGCTGC AGGGATTAAG 
GTGAACTCCA CCATTAATGA GCTGCTGGTG GCTCGCTTCG CGGATGAAGA CCATACCATT 
GACTTTGATA ACTTTGTGAG ATGCCTTCTG CGCCTGGAGA TCATGTTCAA GATCTTCAAC 
CAGATGGACA CTGAGAAGAC CGGAGTGGTG ACTCTGAAGA TGGACACTTG GCTTTCCCTG 
ACTGTGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn2 ( XM_031901617.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757477.1 Xenopus tropicalis calpain 2 (capn2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa57968
Clone ID Related Accession (Same CDS sequence)	XM_031901617.1
Accession Version	XM_031901617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpain-2 catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGGG TGGCTGATAG GCTGGCCAAG GAGCGGGCAC TGGCAAGTGG CCTTGGCACC 
AATGAGAAGG CGCTGCCCTA CCTGAGCCAG GACTATGAGG CGCTGCGCAA TGAGTGTCTC 
CAGTCTGGGA CCCTGTTTAA GGACCTCAGC TTCCCGGCCA TCCACTCCTC CCTGGGGTTC 
AAAGAGCTGG GGCAGCATTC GGCCAAATGC CAAGGGGTGG TATGGAAGAG ACCCAAGGAC 
CTGTGCCCAG ACCCTCAGTT CATCGCAGGA GGAGCGACTC GCACTGATAT CTGCCAAGGA 
GCCCTGGGTG ACTGCTGGTT ACTTGCGGCC ATTGCCTCCC TCACCCTGAA TGAGCAGATT 
CTGTCCCGGG TTGTTCCAAA GGATCAGAGC TTCCAAGAGA ACTATGCCGG AATATTCCAC 
TTCCAGTTCT GGCAGTACGG TGAGTGGGTG GATGTTGTCA TTGATGATCG TCTGCCAACC 
AAGGATGGGA AGCTGCTTTT TGTGCATTCA GCGGAGGGGA GTGAGTTCTG GAGCGCACTC 
ATGGAGAAGG CTTATGCCAA ACTGAACGGA TCCTATGAAG CTCTCTCGGG TGGGACCACC 
ACCGAAGGGT TTGAAGATTT CACAGGTGGA CTTGCAGAGT GGTATGAACT GAGGAAGGCC 
CCACCCAATC TGTTCAAGAT TATCCAGAAA GCTCTGAGGA CTGGATCTCT GCTGGGCTGC 
TCTATAGATA TCACAAGTGC AGCGGAGACA GAAGCCATCA CATTCCAGAA GCTCGTGAAG 
GGCCATGCCT ACTCTGTGAC TGCAGCAGAA GAGGTGCAAT ATCGAGGGAA CCTGGAAAAG 
CTGATCAGAA TCAGAAATCC CTGGGGGGAA GTGGAATGGA CCGGGGCCTG GAGTGACAAT 
TCCAGCGAGT GGAATTCTGT GGATCCGGAT GTGCGGGAAA AGTTGATCAA GAGATGTGAC 
GATGGAGAGT TTTGGATGTC CTTCAATGAC TTCTTGAGAA ACTATTCAAG ACTGGAGATC 
TGCAATCTGA CCCCGGACAC TCTGGCCTCT GACAAATACA GTAAGTGGAG TCTGACCAAG 
ATGGACGGCA CGTGGAGGAG AGGCTCGACA GCCGGCGGAT GCAGGAATTT CCCAGAAACC 
TTCTGGATGA ACCCCCAGTA TGTGATTAAG CTGGAAGAAG CAGATGATGA TCCTGATGAT 
AATGAGGAAG GCTGCACCTT TATTGTCGGT CTGATACAGA AGAACCGCAG GAAGCAGAGG 
AAGATGGGAG AGGACATGCA CACCATTGGC TTTGCCATCT ATGAGCTCAC TGGCCAGAAG 
GACATTCGCC TGAGCCGAGA TTACATTCTA AGGAACAAGA TTCGGGAAAA ATCCGACACC 
TATATAAACC TGCGCGAGGT GCTGAACAGA TTCCGGCTGC CCCCAGGGGA GTACGTCATT 
CTGCCTTCCA CATTCGAACC CCACAAAGTC GGAGACTTCT GCCTGCGGGT CTTCTCTGAA 
AAGAACGCCG AGTCCAAGTG CCAAGACAGA GTTCCTGATG ATGAAATCAA GGCTGACATT 
GATGAGGACG TCAATGAAGA TGCCATCGAC CCCAACTTCA AAAGGATGTT CCTGGCGCTG 
GCAGGAGATG ACAAAGAGAT CTCTCCGCAT GAACTCTTTA ACATCCTACA GAAGGTGATA 
TCCAAGCGAG AAGACATAAA GTCCGATGGG TTCAGCATAG AGACCTGTAG GACAATTGTT 
GACCTGCTGG ATTCTGATGG TACAGGGAAA CTTGGCCTTA AGGAGTTCAA AATCCTATGG 
ACAAAAATAC TGAAGTACCA GAAAATATAC AGCAGCGTGG ATAGGGACCA CTCCGGCACT 
ATTAATTCCT ATGAGATGCG GGGAGCCCTG GAAGCTGCAG GGATTAAGGT GAACTCCACC 
ATTAATGAGC TGCTGGTGGC TCGCTTCGCG GATGAAGACC ATACCATTGA CTTTGATAAC 
TTTGTGAGAT GCCTTCTGCG CCTGGAGATC ATGTTCAAGA TCTTCAACCA GATGGACACT 
GAGAAGACCG GAGTGGTGAC TCTGAAGATG GACACTTGGC TTTCCCTGAC TGTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757477.1
CDS	217..2316
Translation	MSGVADRLAKERALASGLGTNEKALPYLSQDYEALRNECLQSGTLFKDLSFPAIHSSLGFKELGQHSAKCQGVVWKRPKDLCPDPQFIAGGATRTDICQGALGDCWLLAAIASLTLNEQILSRVVPKDQSFQENYAGIFHFQFWQYGEWVDVVIDDRLPTKDGKLLFVHSAEGSEFWSALMEKAYAKLNGSYEALSGGTTTEGFEDFTGGLAEWYELRKAPPNLFKIIQKALRTGSLLGCSIDITSAAETEAITFQKLVKGHAYSVTAAEEVQYRGNLEKLIRIRNPWGEVEWTGAWSDNSSEWNSVDPDVREKLIKRCDDGEFWMSFNDFLRNYSRLEICNLTPDTLASDKYSKWSLTKMDGTWRRGSTAGGCRNFPETFWMNPQYVIKLEEADDDPDDNEEGCTFIVGLIQKNRRKQRKMGEDMHTIGFAIYELTGQKDIRLSRDYILRNKIREKSDTYINLREVLNRFRLPPGEYVILPSTFEPHKVGDFCLRVFSEKNAESKCQDRVPDDEIKADIDEDVNEDAIDPNFKRMFLALAGDDKEISPHELFNILQKVISKREDIKSDGFSIETCRTIVDLLDSDGTGKLGLKEFKILWTKILKYQKIYSSVDRDHSGTINSYEMRGALEAAGIKVNSTINELLVARFADEDHTIDFDNFVRCLLRLEIMFKIFNQMDTEKTGVVTLKMDTWLSLTVI

Target ORF information:

RefSeq Version	XM_031901617.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpain 2 (capn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901617.1

ORF Insert Sequence:

ATGAGCGGGG TGGCTGATAG GCTGGCCAAG GAGCGGGCAC TGGCAAGTGG CCTTGGCACC 
AATGAGAAGG CGCTGCCCTA CCTGAGCCAG GACTATGAGG CGCTGCGCAA TGAGTGTCTC 
CAGTCTGGGA CCCTGTTTAA GGACCTCAGC TTCCCGGCCA TCCACTCCTC CCTGGGGTTC 
AAAGAGCTGG GGCAGCATTC GGCCAAATGC CAAGGGGTGG TATGGAAGAG ACCCAAGGAC 
CTGTGCCCAG ACCCTCAGTT CATCGCAGGA GGAGCGACTC GCACTGATAT CTGCCAAGGA 
GCCCTGGGTG ACTGCTGGTT ACTTGCGGCC ATTGCCTCCC TCACCCTGAA TGAGCAGATT 
CTGTCCCGGG TTGTTCCAAA GGATCAGAGC TTCCAAGAGA ACTATGCCGG AATATTCCAC 
TTCCAGTTCT GGCAGTACGG TGAGTGGGTG GATGTTGTCA TTGATGATCG TCTGCCAACC 
AAGGATGGGA AGCTGCTTTT TGTGCATTCA GCGGAGGGGA GTGAGTTCTG GAGCGCACTC 
ATGGAGAAGG CTTATGCCAA ACTGAACGGA TCCTATGAAG CTCTCTCGGG TGGGACCACC 
ACCGAAGGGT TTGAAGATTT CACAGGTGGA CTTGCAGAGT GGTATGAACT GAGGAAGGCC 
CCACCCAATC TGTTCAAGAT TATCCAGAAA GCTCTGAGGA CTGGATCTCT GCTGGGCTGC 
TCTATAGATA TCACAAGTGC AGCGGAGACA GAAGCCATCA CATTCCAGAA GCTCGTGAAG 
GGCCATGCCT ACTCTGTGAC TGCAGCAGAA GAGGTGCAAT ATCGAGGGAA CCTGGAAAAG 
CTGATCAGAA TCAGAAATCC CTGGGGGGAA GTGGAATGGA CCGGGGCCTG GAGTGACAAT 
TCCAGCGAGT GGAATTCTGT GGATCCGGAT GTGCGGGAAA AGTTGATCAA GAGATGTGAC 
GATGGAGAGT TTTGGATGTC CTTCAATGAC TTCTTGAGAA ACTATTCAAG ACTGGAGATC 
TGCAATCTGA CCCCGGACAC TCTGGCCTCT GACAAATACA GTAAGTGGAG TCTGACCAAG 
ATGGACGGCA CGTGGAGGAG AGGCTCGACA GCCGGCGGAT GCAGGAATTT CCCAGAAACC 
TTCTGGATGA ACCCCCAGTA TGTGATTAAG CTGGAAGAAG CAGATGATGA TCCTGATGAT 
AATGAGGAAG GCTGCACCTT TATTGTCGGT CTGATACAGA AGAACCGCAG GAAGCAGAGG 
AAGATGGGAG AGGACATGCA CACCATTGGC TTTGCCATCT ATGAGCTCAC TGGCCAGAAG 
GACATTCGCC TGAGCCGAGA TTACATTCTA AGGAACAAGA TTCGGGAAAA ATCCGACACC 
TATATAAACC TGCGCGAGGT GCTGAACAGA TTCCGGCTGC CCCCAGGGGA GTACGTCATT 
CTGCCTTCCA CATTCGAACC CCACAAAGTC GGAGACTTCT GCCTGCGGGT CTTCTCTGAA 
AAGAACGCCG AGTCCAAGTG CCAAGACAGA GTTCCTGATG ATGAAATCAA GGCTGACATT 
GATGAGGACG TCAATGAAGA TGCCATCGAC CCCAACTTCA AAAGGATGTT CCTGGCGCTG 
GCAGGAGATG ACAAAGAGAT CTCTCCGCAT GAACTCTTTA ACATCCTACA GAAGGTGATA 
TCCAAGCGAG AAGACATAAA GTCCGATGGG TTCAGCATAG AGACCTGTAG GACAATTGTT 
GACCTGCTGG ATTCTGATGG TACAGGGAAA CTTGGCCTTA AGGAGTTCAA AATCCTATGG 
ACAAAAATAC TGAAGTACCA GAAAATATAC AGCAGCGTGG ATAGGGACCA CTCCGGCACT 
ATTAATTCCT ATGAGATGCG GGGAGCCCTG GAAGCTGCAG GGATTAAGGT GAACTCCACC 
ATTAATGAGC TGCTGGTGGC TCGCTTCGCG GATGAAGACC ATACCATTGA CTTTGATAAC 
TTTGTGAGAT GCCTTCTGCG CCTGGAGATC ATGTTCAAGA TCTTCAACCA GATGGACACT 
GAGAAGACCG GAGTGGTGAC TCTGAAGATG GACACTTGGC TTTCCCTGAC TGTGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn2 ( XM_031901618.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757478.1 Xenopus tropicalis calpain 2 (capn2), transcript variant X3, mRNA.

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CloneID	OXa57969
Clone ID Related Accession (Same CDS sequence)	XM_031901618.1
Accession Version	XM_031901618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpain-2 catalytic subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGGG TGGCTGATAG GCTGGCCAAG GAGCGGGCAC TGGCAAGTGG CCTTGGCACC 
AATGAGAAGG CGCTGCCCTA CCTGAGCCAG GACTATGAGG CGCTGCGCAA TGAGTGTCTC 
CAGTCTGGGA CCCTGTTTAA GGACCTCAGC TTCCCGGCCA TCCACTCCTC CCTGGGGTTC 
AAAGAGCTGG GGCAGCATTC GGCCAAATGC CAAGGGGTGG TATGGAAGAG ACCCAAGGAC 
CTGTGCCCAG ACCCTCAGTT CATCGCAGGA GGAGCGACTC GCACTGATAT CTGCCAAGGA 
GCCCTGGGTG ACTGCTGGTT ACTTGCGGCC ATTGCCTCCC TCACCCTGAA TGAGCAGATT 
CTGTCCCGGG TTGTTCCAAA GGATCAGAGC TTCCAAGAGA ACTATGCCGG AATATTCCAC 
TTCCAGTTCT GGCAGTACGG TGAGTGGGTG GATGTTGTCA TTGATGATCG TCTGCCAACC 
AAGGATGGGA AGCTGCTTTT TGTGCATTCA GCGGAGGGGA GTGAGTTCTG GAGCGCACTC 
ATGGAGAAGG CTTATGCCAA ACTGAACGGA TCCTATGAAG CTCTCTCGGG TGGGACCACC 
ACCGAAGGGT TTGAAGATTT CACAGGTGGA CTTGCAGAGT GGTATGAACT GAGGAAGGCC 
CCACCCAATC TGTTCAAGAT TATCCAGAAA GCTCTGAGGA CTGGATCTCT GCTGGGCTGC 
TCTATAGATA TCACAAGTGC AGCGGAGACA GAAGCCATCA CATTCCAGAA GCTCGTGAAG 
GGCCATGCCT ACTCTGTGAC TGCAGCAGAA GAGGTGCAAT ATCGAGGGAA CCTGGAAAAG 
CTGATCAGAA TCAGAAATCC CTGGGGGGAA GTGGAATGGA CCGGGGCCTG GAGTGACAAT 
TCCAGCGAGT GGAATTCTGT GGATCCGGAT GTGCGGGAAA AGTTGATCAA GAGATGTGAC 
GATGGAGAGT TTTGGATGTC CTTCAATGAC TTCTTGAGAA ACTATTCAAG ACTGGAGATC 
TGCAATCTGA CCCCGGACAC TCTGGCCTCT GACAAATACA GTAAGTGGAG TCTGACCAAG 
ATGGACGGCA CGTGGAGGAG AGGCTCGACA GCCGGCGGAT GCAGGAATTT CCCAGAAACC 
TTCTGGATGA ACCCCCAGTA TGTGATTAAG CTGGAAGAAG CAGATGATGA TCCTGATGAT 
AATGAGGAAG GCTGCACCTT TATTGTCGGT CTGATACAGA AGAACCGCAG GAAGCAGAGG 
AAGATGGGAG AGGACATGCA CACCATTGGC TTTGCCATCT ATGAGCTCAC TGGCCAGAAG 
GACATTCGCC TGAGCCGAGA TTACATTCTA AGGAACAAGA TTCGGGAAAA ATCCGACACC 
TATATAAACC TGCGCGAGGT GCTGAACAGA TTCCGGCTGC CCCCAGGGGA GTACGTCATT 
CTGCCTTCCA CATTCGAACC CCACAAAGTC GGAGACTTCT GCCTGCGGGT CTTCTCTGAA 
AAGAACGCCG AGTCCAAAGT TCCTGATGAT GAAATCAAGG CTGACATTGA TGAGGACGTC 
AATGAAGATG CCATCGACCC CAACTTCAAA AGGATGTTCC TGGCGCTGGC AGGAGATGAC 
AAAGAGATCT CTCCGCATGA ACTCTTTAAC ATCCTACAGA AGGTGATATC CAAGCGAGAA 
GACATAAAGT CCGATGGGTT CAGCATAGAG ACCTGTAGGA CAATTGTTGA CCTGCTGGAT 
TCTGATGGTA CAGGGAAACT TGGCCTTAAG GAGTTCAAAA TCCTATGGAC AAAAATACTG 
AAGTACCAGA AAATATACAG CAGCGTGGAT AGGGACCACT CCGGCACTAT TAATTCCTAT 
GAGATGCGGG GAGCCCTGGA AGCTGCAGGG ATTAAGGTGA ACTCCACCAT TAATGAGCTG 
CTGGTGGCTC GCTTCGCGGA TGAAGACCAT ACCATTGACT TTGATAACTT TGTGAGATGC 
CTTCTGCGCC TGGAGATCAT GTTCAAGATC TTCAACCAGA TGGACACTGA GAAGACCGGA 
GTGGTGACTC TGAAGATGGA CACTTGGCTT TCCCTGACTG TGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757478.1
CDS	217..2304
Translation	MSGVADRLAKERALASGLGTNEKALPYLSQDYEALRNECLQSGTLFKDLSFPAIHSSLGFKELGQHSAKCQGVVWKRPKDLCPDPQFIAGGATRTDICQGALGDCWLLAAIASLTLNEQILSRVVPKDQSFQENYAGIFHFQFWQYGEWVDVVIDDRLPTKDGKLLFVHSAEGSEFWSALMEKAYAKLNGSYEALSGGTTTEGFEDFTGGLAEWYELRKAPPNLFKIIQKALRTGSLLGCSIDITSAAETEAITFQKLVKGHAYSVTAAEEVQYRGNLEKLIRIRNPWGEVEWTGAWSDNSSEWNSVDPDVREKLIKRCDDGEFWMSFNDFLRNYSRLEICNLTPDTLASDKYSKWSLTKMDGTWRRGSTAGGCRNFPETFWMNPQYVIKLEEADDDPDDNEEGCTFIVGLIQKNRRKQRKMGEDMHTIGFAIYELTGQKDIRLSRDYILRNKIREKSDTYINLREVLNRFRLPPGEYVILPSTFEPHKVGDFCLRVFSEKNAESKVPDDEIKADIDEDVNEDAIDPNFKRMFLALAGDDKEISPHELFNILQKVISKREDIKSDGFSIETCRTIVDLLDSDGTGKLGLKEFKILWTKILKYQKIYSSVDRDHSGTINSYEMRGALEAAGIKVNSTINELLVARFADEDHTIDFDNFVRCLLRLEIMFKIFNQMDTEKTGVVTLKMDTWLSLTVI

Target ORF information:

RefSeq Version	XM_031901618.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpain 2 (capn2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901618.1

ORF Insert Sequence:

ATGAGCGGGG TGGCTGATAG GCTGGCCAAG GAGCGGGCAC TGGCAAGTGG CCTTGGCACC 
AATGAGAAGG CGCTGCCCTA CCTGAGCCAG GACTATGAGG CGCTGCGCAA TGAGTGTCTC 
CAGTCTGGGA CCCTGTTTAA GGACCTCAGC TTCCCGGCCA TCCACTCCTC CCTGGGGTTC 
AAAGAGCTGG GGCAGCATTC GGCCAAATGC CAAGGGGTGG TATGGAAGAG ACCCAAGGAC 
CTGTGCCCAG ACCCTCAGTT CATCGCAGGA GGAGCGACTC GCACTGATAT CTGCCAAGGA 
GCCCTGGGTG ACTGCTGGTT ACTTGCGGCC ATTGCCTCCC TCACCCTGAA TGAGCAGATT 
CTGTCCCGGG TTGTTCCAAA GGATCAGAGC TTCCAAGAGA ACTATGCCGG AATATTCCAC 
TTCCAGTTCT GGCAGTACGG TGAGTGGGTG GATGTTGTCA TTGATGATCG TCTGCCAACC 
AAGGATGGGA AGCTGCTTTT TGTGCATTCA GCGGAGGGGA GTGAGTTCTG GAGCGCACTC 
ATGGAGAAGG CTTATGCCAA ACTGAACGGA TCCTATGAAG CTCTCTCGGG TGGGACCACC 
ACCGAAGGGT TTGAAGATTT CACAGGTGGA CTTGCAGAGT GGTATGAACT GAGGAAGGCC 
CCACCCAATC TGTTCAAGAT TATCCAGAAA GCTCTGAGGA CTGGATCTCT GCTGGGCTGC 
TCTATAGATA TCACAAGTGC AGCGGAGACA GAAGCCATCA CATTCCAGAA GCTCGTGAAG 
GGCCATGCCT ACTCTGTGAC TGCAGCAGAA GAGGTGCAAT ATCGAGGGAA CCTGGAAAAG 
CTGATCAGAA TCAGAAATCC CTGGGGGGAA GTGGAATGGA CCGGGGCCTG GAGTGACAAT 
TCCAGCGAGT GGAATTCTGT GGATCCGGAT GTGCGGGAAA AGTTGATCAA GAGATGTGAC 
GATGGAGAGT TTTGGATGTC CTTCAATGAC TTCTTGAGAA ACTATTCAAG ACTGGAGATC 
TGCAATCTGA CCCCGGACAC TCTGGCCTCT GACAAATACA GTAAGTGGAG TCTGACCAAG 
ATGGACGGCA CGTGGAGGAG AGGCTCGACA GCCGGCGGAT GCAGGAATTT CCCAGAAACC 
TTCTGGATGA ACCCCCAGTA TGTGATTAAG CTGGAAGAAG CAGATGATGA TCCTGATGAT 
AATGAGGAAG GCTGCACCTT TATTGTCGGT CTGATACAGA AGAACCGCAG GAAGCAGAGG 
AAGATGGGAG AGGACATGCA CACCATTGGC TTTGCCATCT ATGAGCTCAC TGGCCAGAAG 
GACATTCGCC TGAGCCGAGA TTACATTCTA AGGAACAAGA TTCGGGAAAA ATCCGACACC 
TATATAAACC TGCGCGAGGT GCTGAACAGA TTCCGGCTGC CCCCAGGGGA GTACGTCATT 
CTGCCTTCCA CATTCGAACC CCACAAAGTC GGAGACTTCT GCCTGCGGGT CTTCTCTGAA 
AAGAACGCCG AGTCCAAAGT TCCTGATGAT GAAATCAAGG CTGACATTGA TGAGGACGTC 
AATGAAGATG CCATCGACCC CAACTTCAAA AGGATGTTCC TGGCGCTGGC AGGAGATGAC 
AAAGAGATCT CTCCGCATGA ACTCTTTAAC ATCCTACAGA AGGTGATATC CAAGCGAGAA 
GACATAAAGT CCGATGGGTT CAGCATAGAG ACCTGTAGGA CAATTGTTGA CCTGCTGGAT 
TCTGATGGTA CAGGGAAACT TGGCCTTAAG GAGTTCAAAA TCCTATGGAC AAAAATACTG 
AAGTACCAGA AAATATACAG CAGCGTGGAT AGGGACCACT CCGGCACTAT TAATTCCTAT 
GAGATGCGGG GAGCCCTGGA AGCTGCAGGG ATTAAGGTGA ACTCCACCAT TAATGAGCTG 
CTGGTGGCTC GCTTCGCGGA TGAAGACCAT ACCATTGACT TTGATAACTT TGTGAGATGC 
CTTCTGCGCC TGGAGATCAT GTTCAAGATC TTCAACCAGA TGGACACTGA GAAGACCGGA 
GTGGTGACTC TGAAGATGGA CACTTGGCTT TCCCTGACTG TGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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capn2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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