CAPN2L cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAPN2L
Entrez Gene ID	550505
Full Name	calpain 2, (m/II) large subunit, like
Synonyms	CH73-80N6.1,wu:fb09d04,wu:fb26a06 gene,xx:hac23b03,zgc:112420
General protein information	Preferred Names calpain 2, (m/II) large subunit, like Names calpain-2 catalytic subunit
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001017807.1	NP_001017807.1	calpain-2 catalytic subunit
XM_009296386.2	XP_009294661.1	calpain-2 catalytic subunit isoform X1

Pathways from BioSystems

Homologies

	CAPN2	XP_001098172.1
Mus musculus (house mouse)	Capn2	NP_033924.2
Danio rerio (zebrafish)	zgc:92480	NP_001003485.1
Rattus norvegicus (Norway rat)	Capn2	NP_058812.1
Xenopus tropicalis (tropical clawed frog)	capn2.2	NP_001005446.1
Homo sapiens (human)	CAPN2	NP_001739.2
Pan troglodytes (chimpanzee)	CAPN2	XP_003308813.1
Danio rerio (zebrafish)	capn2l	NP_001017807.1
Canis lupus familiaris (dog)	CAPN2	XP_005622713.1
Bos taurus (cattle)	CAPN2	NP_001096556.1
Gallus gallus (chicken)	CAPN2	NP_990411.1

Gene Ontology
Bibliography

Related articles in PubMed

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.
Tao T, Shi H, Guan Y, Huang D, Chen Y, Lane DP, Chen J, Peng J
Cell research23(5)620-34(2013 May)

Comprehensive profiling of zebrafish hepatic proximal promoter CpG island methylation and its modification during chemical carcinogenesis.
Mirbahai L, Williams TD, Zhan H, Gong Z, Chipman JK
BMC genomics123(2011 Jan)

Characterization and comparative expression of zebrafish calpain system genes during early development.
Lepage SE, Bruce AE
Developmental dynamics : an official publication of the American Association of Anatomists237(3)819-29(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CAPN2L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAPN2L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36429	NM_001017807.1 Latest version!	Danio rerio calpain 2, (m/II) large subunit, like (capn2l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa41778	XM_009296386.2 Latest version!	Danio rerio calpain 2, (m/II) large subunit, like (capn2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAPN2L ( NM_001017807.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001017807.1 Danio rerio calpain 2, (m/II) large subunit, like (capn2l), mRNA.

Price & Availability↑

CloneID	ODa36429
Clone ID Related Accession (Same CDS sequence)	NM_001017807.1
Accession Version	NM_001017807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-25
Organism	Danio rerio(zebrafish)
Product	calpain-2 catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC093295.1. ##Evidence-Data-START## Transcript exon combination :: BC093295.1, BC154357.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGGAA TCGCTACAAA ACTCCAAAAG AACCGGGCTC GAGCGGCGGG CATCGGTACC 
AACGCTCAAG CTGCCAAATT CCTCAACCAG GACTACGAAG CCCTCAAACG AGAGTGTCTG 
GAGTCCGGAC GCCTGTTTCA GGATGGCATG TTTGAAGCCA ATGTGTCTGC CCTTGGGTTT 
AAGGAACTGG GACCAAACTC GTCTAAAGTG CGAGGGGTTG AGTGGCTGAG ACCCAAACAA 
CTGACGTCCA ACCCAACATT CATTAGTGGG GGAGCGACTA GAACAGACAT CTGCCAGGGT 
GCTTTGGGTG ACTGTTGGCT TCTAGCGGCC ATCGCTTCTC TTACTCTCAA CCAGGATGTG 
TTTGCTCGTG TCGTGCCGGC TGGTCAGAGT TTTGATGGCG ACTATGCTGG GATATTTCAT 
TTTCAGTTCT GGCAGTTTGG TGAATGGGTG GATGTGGTCA TTGACGACCG ACTGCCGGCC 
AGAAAAGGAG AGCTGCTATT CGTCCACTCG GCCGAGGGCT CTGAGTTTTG GAGCGCTCTA 
TTAGAAAAAG CCTATGCAAA GCTGAATGGC TGCTATGAAG CTCTTTCTGG CGGCACCACC 
ACTGAAGGTT TTGAGGATTT CACTGGAGGC ATCGCTGAGG TTCACGAACT GCCCAAAGCG 
GGTCCAAACC TCTTCAAAAC TATCCAGAAG GCTTTGAGTT GGGGCTCATT GCTGGGCTGC 
TCCATAGATA TCACAAGCTC ATCTGACTCA GAGGCCATTA CTAGTCAGAA ACTGGTGAAA 
GGACACGCCT ACTCGGTTAC AGGAGCTGAG GAGGTTGAAT ACAGGGGTGA CTTAACCAAA 
CTGATTCGCA TCAGGAACCC CTGGGGGCAG GTGGAGTGGA CGGGACCCTG GAGTGATGGG 
TCATCAGAAT GGCGTCAGAT CAGTGACAGC GACAGAGAAA GACTGAGCTC CAAAGCCGAA 
GATGGAGAAT TCTGGATGTC TTTCTCGGAC TTCATGCGTC ATTACTCGCG GGTGGAGATC 
TGCAACCTGA CACCCGATGC CCTGACAGAC GAAAGTGTTA ATAAATGGGC TCTGTCCAAG 
TTTGATGGCA ACTGGAGGAA TGGATCAACT GCTGGAGGCT GCAGGAATTA CCCCAACTCG 
TTCTGGATGA ATCCTCAGTT TTTGATCAAG CTGGAGGAGC AGGACGATGA CCCGACTGAT 
AATGAAGCAG GATGCAGCTT CGTTGTCGGA TTGATCCAGA AGAACCGCCG CAAAATGAGG 
AAGGTCGGAG AGGACATGAA CACCATCGGC TTCGCAATCT ATGAGGTTCC TGACGAGTTT 
GTGGGACAGA GGAACGTTCA CCTGGATCGT AACTTCTTTG TGCGACATGC TTCAGCTGCT 
CGCTCCGAGA CCTTCATCAA CCTGCGGGAG GTTTGCTCTC GCTTCTGTCT GCCGCCCGGC 
GAGTACCTCA TCGTACCCTC CACCTTTGAG CCCAACAAAG ATGGCGACTT CTGTGTGCGC 
GTTTTCTCCG AAAAACAAGC AGAGTTCCAA GAGCTTGATG ATCCTGTCGA GTCCAAGGTC 
GCAGAGATTG AGATAAAAGA GGGAGATATC GATAGCCGGT TCAGGAATCT GTTCAAGCAG 
CTTGCTGGAG CTGACAGTGA GATCTCTGCA TTTGAGCTGC AAAAAATCCT GAACAATGTG 
ATAGCTAAAC GCAAAGATAT TAAAACTGAC GGATTTAGCT TGGAGACCTG CCGCAACATG 
GTGAACCTAC TGGACAAAGA CGGCACTGGA AAACTAGGCC TGCTTGAATT TAAGATTCTG 
TGGACTAAAA TTGAGCTGTT TGTGGACGTC TACAGTAAAA ATGACAAAGA CCAGTCCGGC 
ACAATGAGCT CTATGGAGAT GAGAGAAGCC GTAGAGAAAG CAGGGTTTTC TCTGAATAAC 
GCTCTGCACC AGATCCTTGT TGCACGATAC AGCGAACCCA ACCTCACCAT CGACTTTGAC 
AACTTTGTGG CCTGTCTTAT CCGCCTCGAA TGCATGTTCA AGGCATTTAA GGTGCTGGAC 
AAGGACAAGA ATGGCACAGT CGAGCTGAAC ATGATGGAGT GGCTGAATGT TTCCATGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017807.1
CDS	25..2127
Translation	MSGIATKLQKNRARAAGIGTNAQAAKFLNQDYEALKRECLESGRLFQDGMFEANVSALGFKELGPNSSKVRGVEWLRPKQLTSNPTFISGGATRTDICQGALGDCWLLAAIASLTLNQDVFARVVPAGQSFDGDYAGIFHFQFWQFGEWVDVVIDDRLPARKGELLFVHSAEGSEFWSALLEKAYAKLNGCYEALSGGTTTEGFEDFTGGIAEVHELPKAGPNLFKTIQKALSWGSLLGCSIDITSSSDSEAITSQKLVKGHAYSVTGAEEVEYRGDLTKLIRIRNPWGQVEWTGPWSDGSSEWRQISDSDRERLSSKAEDGEFWMSFSDFMRHYSRVEICNLTPDALTDESVNKWALSKFDGNWRNGSTAGGCRNYPNSFWMNPQFLIKLEEQDDDPTDNEAGCSFVVGLIQKNRRKMRKVGEDMNTIGFAIYEVPDEFVGQRNVHLDRNFFVRHASAARSETFINLREVCSRFCLPPGEYLIVPSTFEPNKDGDFCVRVFSEKQAEFQELDDPVESKVAEIEIKEGDIDSRFRNLFKQLAGADSEISAFELQKILNNVIAKRKDIKTDGFSLETCRNMVNLLDKDGTGKLGLLEFKILWTKIELFVDVYSKNDKDQSGTMSSMEMREAVEKAGFSLNNALHQILVARYSEPNLTIDFDNFVACLIRLECMFKAFKVLDKDKNGTVELNMMEWLNVSML

Target ORF information:

RefSeq Version	NM_001017807.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpain 2, (m/II) large subunit, like (capn2l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017807.1

ORF Insert Sequence:

ATGTCAGGAA TCGCTACAAA ACTCCAAAAG AACCGGGCTC GAGCGGCGGG CATCGGTACC 
AACGCTCAAG CTGCCAAATT CCTCAACCAG GACTACGAAG CCCTCAAACG AGAGTGTCTG 
GAGTCCGGAC GCCTGTTTCA GGATGGCATG TTTGAAGCCA ATGTGTCTGC CCTTGGGTTT 
AAGGAACTGG GACCAAACTC GTCTAAAGTG CGAGGGGTTG AGTGGCTGAG ACCCAAACAA 
CTGACGTCCA ACCCAACATT CATTAGTGGG GGAGCGACTA GAACAGACAT CTGCCAGGGT 
GCTTTGGGTG ACTGTTGGCT TCTAGCGGCC ATCGCTTCTC TTACTCTCAA CCAGGATGTG 
TTTGCTCGTG TCGTGCCGGC TGGTCAGAGT TTTGATGGCG ACTATGCTGG GATATTTCAT 
TTTCAGTTCT GGCAGTTTGG TGAATGGGTG GATGTGGTCA TTGACGACCG ACTGCCGGCC 
AGAAAAGGAG AGCTGCTATT CGTCCACTCG GCCGAGGGCT CTGAGTTTTG GAGCGCTCTA 
TTAGAAAAAG CCTATGCAAA GCTGAATGGC TGCTATGAAG CTCTTTCTGG CGGCACCACC 
ACTGAAGGTT TTGAGGATTT CACTGGAGGC ATCGCTGAGG TTCACGAACT GCCCAAAGCG 
GGTCCAAACC TCTTCAAAAC TATCCAGAAG GCTTTGAGTT GGGGCTCATT GCTGGGCTGC 
TCCATAGATA TCACAAGCTC ATCTGACTCA GAGGCCATTA CTAGTCAGAA ACTGGTGAAA 
GGACACGCCT ACTCGGTTAC AGGAGCTGAG GAGGTTGAAT ACAGGGGTGA CTTAACCAAA 
CTGATTCGCA TCAGGAACCC CTGGGGGCAG GTGGAGTGGA CGGGACCCTG GAGTGATGGG 
TCATCAGAAT GGCGTCAGAT CAGTGACAGC GACAGAGAAA GACTGAGCTC CAAAGCCGAA 
GATGGAGAAT TCTGGATGTC TTTCTCGGAC TTCATGCGTC ATTACTCGCG GGTGGAGATC 
TGCAACCTGA CACCCGATGC CCTGACAGAC GAAAGTGTTA ATAAATGGGC TCTGTCCAAG 
TTTGATGGCA ACTGGAGGAA TGGATCAACT GCTGGAGGCT GCAGGAATTA CCCCAACTCG 
TTCTGGATGA ATCCTCAGTT TTTGATCAAG CTGGAGGAGC AGGACGATGA CCCGACTGAT 
AATGAAGCAG GATGCAGCTT CGTTGTCGGA TTGATCCAGA AGAACCGCCG CAAAATGAGG 
AAGGTCGGAG AGGACATGAA CACCATCGGC TTCGCAATCT ATGAGGTTCC TGACGAGTTT 
GTGGGACAGA GGAACGTTCA CCTGGATCGT AACTTCTTTG TGCGACATGC TTCAGCTGCT 
CGCTCCGAGA CCTTCATCAA CCTGCGGGAG GTTTGCTCTC GCTTCTGTCT GCCGCCCGGC 
GAGTACCTCA TCGTACCCTC CACCTTTGAG CCCAACAAAG ATGGCGACTT CTGTGTGCGC 
GTTTTCTCCG AAAAACAAGC AGAGTTCCAA GAGCTTGATG ATCCTGTCGA GTCCAAGGTC 
GCAGAGATTG AGATAAAAGA GGGAGATATC GATAGCCGGT TCAGGAATCT GTTCAAGCAG 
CTTGCTGGAG CTGACAGTGA GATCTCTGCA TTTGAGCTGC AAAAAATCCT GAACAATGTG 
ATAGCTAAAC GCAAAGATAT TAAAACTGAC GGATTTAGCT TGGAGACCTG CCGCAACATG 
GTGAACCTAC TGGACAAAGA CGGCACTGGA AAACTAGGCC TGCTTGAATT TAAGATTCTG 
TGGACTAAAA TTGAGCTGTT TGTGGACGTC TACAGTAAAA ATGACAAAGA CCAGTCCGGC 
ACAATGAGCT CTATGGAGAT GAGAGAAGCC GTAGAGAAAG CAGGGTTTTC TCTGAATAAC 
GCTCTGCACC AGATCCTTGT TGCACGATAC AGCGAACCCA ACCTCACCAT CGACTTTGAC 
AACTTTGTGG CCTGTCTTAT CCGCCTCGAA TGCATGTTCA AGGCATTTAA GGTGCTGGAC 
AAGGACAAGA ATGGCACAGT CGAGCTGAAC ATGATGGAGT GGCTGAATGT TTCCATGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN2L ( XM_009296386.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294661.1 Danio rerio calpain 2, (m/II) large subunit, like (capn2l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41778
Clone ID Related Accession (Same CDS sequence)	XM_009296386.2
Accession Version	XM_009296386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpain-2 catalytic subunit isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA TCGCTACAAA ACTCCAAAAG AACCGGGCTC GAGCGGCGGG CATCGGTACC 
AACGCTCAAG CTGCCAAATT CCTCAACCAG GACTACGAAG CCCTCAAACG AGAGTGTCTG 
GAGTCCGGAC GCCTGTTTCA GGATGGCATG TTTGAAGCCA ATGTGTCTGC CCTTGGGTTT 
AAGGAACTGG GACCAAACTC GTCTAAAGTG CGAGGGGTTG AGTGGCTGAG ACCCAAACAA 
CTGACGTCCA ACCCAACATT CATTAGTGGG GGAGCGACTA GAACAGACAT CTGCCAGGGT 
GCTTTGGGTG ACTGTTGGCT TCTAGCGGCC ATCGCTTCTC TTACTCTCAA CCAGGATGTG 
TTTGCTCGTG TCGTGCCGGC TGGTCAGAGT TTTGATGGCG ACTATGCTGG GATATTTCAT 
TTTCAGTTCT GGCAGTTTGG TGAATGGGTG GATGTGGTCA TTGACGACCG ACTGCCGGCC 
AGAAAAGGAG AGCTGCTATT CGTCCACTCG GCCGAGGGCT CTGAGTTTTG GAGCGCTCTA 
TTAGAAAAAG CCTATGCAAA GCTGAATGGC TGCTATGAAG CTCTTTCTGG CGGCACCACC 
ACTGAAGGTT TTGAGGATTT CACTGGAGGC ATCGCTGAGG TTCACGAACT GCCCAAAGCG 
GGTCCAAACC TCTTCAAAAC TATCCAGAAG GCTTTGAGTT GGGGCTCATT GCTGGGCTGC 
TCCATAGATA TCACAAGCTC ATCTGACTCA GAGGCCATTA CTAGTCAGAA ACTGGTGAAA 
GGACACGCCT ACTCGGTTAC AGGAGCTGAG GAGGTTGAAT ACAGGGGTGA CTTAACCAAA 
CTGATTCGCA TCAGGAACCC CTGGGGGCAG GTGGAGTGGA CGGGACCCTG GAGTGATGGG 
TCATCAGAAT GGCGTCAGAT CAGTGACAGC GACAGAGAAA GACTGAGCTC CAAAGCCGAA 
GATGGAGAAT TCTGGATGTC TTTCTCGGAC TTCATGCGTC ATTACTCGCG GGTGGAGATC 
TGCAACCTGA CACCCGATGC TCTGACAGAC GAAAGTGTTA ATAAATGGGC TCTGTCCAAG 
TTTGATGGCA ACTGGAGGAA TGGATCAACT GCTGGAGGCT GCAGGAATTA CCCCAACTCG 
TTCTGGATGA ATCCTCAGTT TTTGATCAAG CTGGAGGAGC AGGACGATGA CCCGACTGAT 
AATGAAGCAG GATGCAGCTT CGTTGTCGGA TTGATCCAGA AGAACCGCCG CAAAATGAGG 
AAGGTCGGAG AGGACATGAA CACCATCGGC TTCGCAATCT ATGAGTTTGT GGGACAGAGG 
AACGTTCACC TGGATCGTAA CTTCTTTGTG CGACATGCTT CAGCTGCTCG CTCCGAGACC 
TTCATCAACC TGCGGGAGGT TTGCTCTCGC TTCTGTCTGC CGCCCGGCGA GTACCTCATC 
GTACCCTCCA CCTTTGAGCC CAACAAAGAT GGCGACTTCT GTGTGCGCGT TTTCTCCGAA 
AAACAAGCAG AGTTCCAAGA GCTTGATGAT CCTGTCGAGT CCAAGGTCGC AGAGATTGAG 
ATAAAAGAGG GAGATATCGA TAGCCGGTTC AGGAATCTGT TCAAGCAGCT TGCTGGAGCT 
GACAGTGAGA TCTCTGCATT TGAGCTGCAA AAAATCCTGA ACAATGTGAT AGCTAAACGC 
AAAGATATTA AAACTGACGG ATTTAGCTTG GAGACCTGCC GCAACATGGT GAACCTACTG 
GACAAAGACG GCACTGGAAA ACTAGGCCTG CTTGAATTTA AGATTCTGTG GACTAAAATT 
GAGCTGTTTG TGGACGTCTA CAGTAAAAAT GACAAAGACC AGTCCGGCAC AATGAGCTCT 
ATGGAGATGA GAGAAGCCGT AGAGAAAGCA GGGTTTTCTC TGAATAACGC TCTGCACCAG 
ATCCTTGTTG CACGATACAG CGAACCCAAC CTCACCATCG ACTTTGACAA CTTTGTGGCC 
TGTCTTATCC GCCTCGAATG CATGTTCAAG GCATTTAAGG TGCTGGACAA GGACAAGAAT 
GGCACAGTCG AGCTGAACAT GATGGAGTGG CTGAATGTTT CCATGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294661.1
CDS	146..2236
Translation	MSGIATKLQKNRARAAGIGTNAQAAKFLNQDYEALKRECLESGRLFQDGMFEANVSALGFKELGPNSSKVRGVEWLRPKQLTSNPTFISGGATRTDICQGALGDCWLLAAIASLTLNQDVFARVVPAGQSFDGDYAGIFHFQFWQFGEWVDVVIDDRLPARKGELLFVHSAEGSEFWSALLEKAYAKLNGCYEALSGGTTTEGFEDFTGGIAEVHELPKAGPNLFKTIQKALSWGSLLGCSIDITSSSDSEAITSQKLVKGHAYSVTGAEEVEYRGDLTKLIRIRNPWGQVEWTGPWSDGSSEWRQISDSDRERLSSKAEDGEFWMSFSDFMRHYSRVEICNLTPDALTDESVNKWALSKFDGNWRNGSTAGGCRNYPNSFWMNPQFLIKLEEQDDDPTDNEAGCSFVVGLIQKNRRKMRKVGEDMNTIGFAIYEFVGQRNVHLDRNFFVRHASAARSETFINLREVCSRFCLPPGEYLIVPSTFEPNKDGDFCVRVFSEKQAEFQELDDPVESKVAEIEIKEGDIDSRFRNLFKQLAGADSEISAFELQKILNNVIAKRKDIKTDGFSLETCRNMVNLLDKDGTGKLGLLEFKILWTKIELFVDVYSKNDKDQSGTMSSMEMREAVEKAGFSLNNALHQILVARYSEPNLTIDFDNFVACLIRLECMFKAFKVLDKDKNGTVELNMMEWLNVSML

Target ORF information:

RefSeq Version	XM_009296386.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpain 2, (m/II) large subunit, like (capn2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296386.2

ORF Insert Sequence:

ATGTCAGGAA TCGCTACAAA ACTCCAAAAG AACCGGGCTC GAGCGGCGGG CATCGGTACC 
AACGCTCAAG CTGCCAAATT CCTCAACCAG GACTACGAAG CCCTCAAACG AGAGTGTCTG 
GAGTCCGGAC GCCTGTTTCA GGATGGCATG TTTGAAGCCA ATGTGTCTGC CCTTGGGTTT 
AAGGAACTGG GACCAAACTC GTCTAAAGTG CGAGGGGTTG AGTGGCTGAG ACCCAAACAA 
CTGACGTCCA ACCCAACATT CATTAGTGGG GGAGCGACTA GAACAGACAT CTGCCAGGGT 
GCTTTGGGTG ACTGTTGGCT TCTAGCGGCC ATCGCTTCTC TTACTCTCAA CCAGGATGTG 
TTTGCTCGTG TCGTGCCGGC TGGTCAGAGT TTTGATGGCG ACTATGCTGG GATATTTCAT 
TTTCAGTTCT GGCAGTTTGG TGAATGGGTG GATGTGGTCA TTGACGACCG ACTGCCGGCC 
AGAAAAGGAG AGCTGCTATT CGTCCACTCG GCCGAGGGCT CTGAGTTTTG GAGCGCTCTA 
TTAGAAAAAG CCTATGCAAA GCTGAATGGC TGCTATGAAG CTCTTTCTGG CGGCACCACC 
ACTGAAGGTT TTGAGGATTT CACTGGAGGC ATCGCTGAGG TTCACGAACT GCCCAAAGCG 
GGTCCAAACC TCTTCAAAAC TATCCAGAAG GCTTTGAGTT GGGGCTCATT GCTGGGCTGC 
TCCATAGATA TCACAAGCTC ATCTGACTCA GAGGCCATTA CTAGTCAGAA ACTGGTGAAA 
GGACACGCCT ACTCGGTTAC AGGAGCTGAG GAGGTTGAAT ACAGGGGTGA CTTAACCAAA 
CTGATTCGCA TCAGGAACCC CTGGGGGCAG GTGGAGTGGA CGGGACCCTG GAGTGATGGG 
TCATCAGAAT GGCGTCAGAT CAGTGACAGC GACAGAGAAA GACTGAGCTC CAAAGCCGAA 
GATGGAGAAT TCTGGATGTC TTTCTCGGAC TTCATGCGTC ATTACTCGCG GGTGGAGATC 
TGCAACCTGA CACCCGATGC TCTGACAGAC GAAAGTGTTA ATAAATGGGC TCTGTCCAAG 
TTTGATGGCA ACTGGAGGAA TGGATCAACT GCTGGAGGCT GCAGGAATTA CCCCAACTCG 
TTCTGGATGA ATCCTCAGTT TTTGATCAAG CTGGAGGAGC AGGACGATGA CCCGACTGAT 
AATGAAGCAG GATGCAGCTT CGTTGTCGGA TTGATCCAGA AGAACCGCCG CAAAATGAGG 
AAGGTCGGAG AGGACATGAA CACCATCGGC TTCGCAATCT ATGAGTTTGT GGGACAGAGG 
AACGTTCACC TGGATCGTAA CTTCTTTGTG CGACATGCTT CAGCTGCTCG CTCCGAGACC 
TTCATCAACC TGCGGGAGGT TTGCTCTCGC TTCTGTCTGC CGCCCGGCGA GTACCTCATC 
GTACCCTCCA CCTTTGAGCC CAACAAAGAT GGCGACTTCT GTGTGCGCGT TTTCTCCGAA 
AAACAAGCAG AGTTCCAAGA GCTTGATGAT CCTGTCGAGT CCAAGGTCGC AGAGATTGAG 
ATAAAAGAGG GAGATATCGA TAGCCGGTTC AGGAATCTGT TCAAGCAGCT TGCTGGAGCT 
GACAGTGAGA TCTCTGCATT TGAGCTGCAA AAAATCCTGA ACAATGTGAT AGCTAAACGC 
AAAGATATTA AAACTGACGG ATTTAGCTTG GAGACCTGCC GCAACATGGT GAACCTACTG 
GACAAAGACG GCACTGGAAA ACTAGGCCTG CTTGAATTTA AGATTCTGTG GACTAAAATT 
GAGCTGTTTG TGGACGTCTA CAGTAAAAAT GACAAAGACC AGTCCGGCAC AATGAGCTCT 
ATGGAGATGA GAGAAGCCGT AGAGAAAGCA GGGTTTTCTC TGAATAACGC TCTGCACCAG 
ATCCTTGTTG CACGATACAG CGAACCCAAC CTCACCATCG ACTTTGACAA CTTTGTGGCC 
TGTCTTATCC GCCTCGAATG CATGTTCAAG GCATTTAAGG TGCTGGACAA GGACAAGAAT 
GGCACAGTCG AGCTGAACAT GATGGAGTGG CTGAATGTTT CCATGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.
Cell research23(5)620-34(2013 May)
Tao T,Shi H,Guan Y,Huang D,Chen Y,Lane DP,Chen J,Peng J

Comprehensive profiling of zebrafish hepatic proximal promoter CpG island methylation and its modification during chemical carcinogenesis.
BMC genomics123(2011 Jan)
Mirbahai L,Williams TD,Zhan H,Gong Z,Chipman JK

Characterization and comparative expression of zebrafish calpain system genes during early development.
Developmental dynamics : an official publication of the American Association of Anatomists237(3)819-29(2008 Mar)
Lepage SE,Bruce AE

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CAPN2L cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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