NEFM cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFM
Entrez Gene ID	281347
Full Name	neurofilament medium
Synonyms	NEF3,NF-M
General protein information	Preferred Names neurofilament medium Names neurofilament medium polypeptide 160 kDa neurofilament protein major neuronal intermediate filament subunit neurofilament 3 neurofilament M subunit neurofilament triplet M protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010808063.1	XP_010806365.1	neurofilament medium polypeptide isoform X2
XM_005210283.3	XP_005210340.1	neurofilament medium polypeptide isoform X1
XM_010808063.2	XP_010806365.2	neurofilament medium polypeptide isoform X2
NM_001244145.1	NP_001231074.1	neurofilament medium polypeptide
XM_024995441.1	XP_024851209.1	neurofilament medium polypeptide isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NEFM	NP_005373.2
Pan troglodytes (chimpanzee)	NEFM	XP_001150727.2
Bos taurus (cattle)	NEFM	NP_001231074.1
	NEFM	XP_001106972.2
Canis lupus familiaris (dog)	NEFM	XP_543237.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of the bovine neurofilament NF-M protein and cDNA sequence, and identification of in vitro and in vivo calpain cleavage sites.
Shaw G, Yang C, Zhang L, Cook P, Pike B, Hill WD
Biochemical and biophysical research communications325(2)619-25(2004 Dec)

Identification of endogenous phosphorylation sites of bovine medium and low molecular weight neurofilament proteins by tandem mass spectrometry.
Trimpin S, Mixon AE, Stapels MD, Kim MY, Spencer PS, Deinzer ML
Biochemistry43(7)2091-105(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NEFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa236921	XM_010808063.2 Latest version!	Bos taurus neurofilament medium (NEFM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa44427	NM_001244145.1 Latest version!	Bos taurus neurofilament medium (NEFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa22185	XM_005210283.3 Latest version!	Bos taurus neurofilament, medium polypeptide (NEFM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa45125	XM_010808063.1 Latest version!	Bos taurus neurofilament, medium polypeptide (NEFM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa61918	XM_024995441.1 Latest version!	Bos taurus neurofilament medium (NEFM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFM ( XM_010808063.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806365.2 Bos taurus neurofilament medium (NEFM), transcript variant X2, mRNA.

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CloneID	OBa236921
Clone ID Related Accession (Same CDS sequence)	XM_010808063.2
Accession Version	XM_010808063.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofilament medium polypeptide isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010808063.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGTC ATCTGCGAGA ATACCAGGAT CTCCTCAACG TCAAGATGGC TCTGGATATT 
GAGATCGCGG CGTACAGGAA ACTCCTGGAG GGTGAAGAGA CCAGATTTAG CACATTTGCA 
GGTAGCATCA CTGGGCCACT GTATACACAC CGACAGCCCT CCATCGCCAT ATCCAGTAAG 
ATTCAGAAAA CCAAGGTAGA GGCTCCCAAG CTAAAGGTCC AACACAAATT TGTTGAGGAG 
ATTATAGAGG AAACCAAGGT GGAAGATGAG AAATCAGAAA TGGAAGAAGC CCTGACGGCC 
ATTACCGAGG AATTGGCCGT TTCCGTGAAA GAGGAGGTCA AGGAAGAGGA GGCTGAAGAA 
AAGGAGGAGA AAGAAGAAGC CGAAGAAGAA GTTGTTGCTG CCAAAAAGTC TCCAGTGAAA 
GCTACTGCAC CTGAACTTAA AGAAGAGGAA GGAGAAAAGG AGGAGGAAGA GGGCCAAGAG 
GAAGAGGAAG AGGAAGAAGA GGCTGCTAAG TCAGACCAAG CCGAGGAAGG AGGATCTGAG 
AAGGAAGGTT CTAGTGAAAA AGAGGAAGGT GAGCAAGAAG AGGAAGGAGA AACAGAGGCT 
GAGGGGGAAG GAGAGGAAGC CGCTGCCGAA GCTAAGGAGG AAAAGAAAAT GGAGGAAAAG 
GCTGAAGAAG TGGCTCCAAA GGAGGAGCTG GCGGCAGAAG CCAAGGTGGA GAAGCCAGAG 
AAAGCCAAGT CCCCAGTGGC CAAGTCCCCA ACAACAAAGT CCCCAACGGC CAAGTCCCCA 
GAGGCAAAGT CCCCAACAGC AAAATCCCCG ACGGCCAAGT CCCCAGTGGC CAAGTCCCCG 
ACGGCCAAGT CCCCGACGGC CAAGTCCCCA GAGGCAAAGT CCCCAGAGGC AAAGTCCCCA 
ACAGCAAAAT CCCCGACGGC CAAGTCCCCA GCAGCAAAGT CCCCAGCGGC AAAATCACCT 
GTGGAGGAAG TGAAACCCAA AGCAGAAGCT GGAGCTGAGA AAGGAGAACA GAAGGAGAAG 
GTGGAGGAAG AAAAGAAAGA AGCAAAGGAA TCTCCCAAGG AAGAGAAGGC AGAGAAAAAG 
GAGGAGAAGG CAAAGGATGT GCCAGAGAAG AAGAAGGCTG AATCCCCAGT GAAGGCTGAG 
TCCCCAGTGA AGGAGGAGGT GCCTGCCAAG CCAGTAAAGG TGAGCCCAGA GAAGGAAGCC 
AAAGAGGAGG AGAAGCCACA GGAGAAAGAG AAGGAGAAGG AGAAAGTGGA AGAGGTGGGA 
GGGAAGGAGG AGGGAGGTTT GAAGGAATCC AGGAAGGAAG ACATAGCCAT CAATGGGGAG 
GTGGAAGGGA AGGAGGAAGA ACAGGAAACT AAGGAGAAAG GCAGTGGGGG AGAAGAGGAG 
AAAGGAGTCG TCACCAACGG GCTAGACGTG AGCCCAGGGG ATGAAAAGAA GGGCGGTGAT 
AAAAGTGAGG AGAAAGTGGT GGTAACCAAA ATGGTGGAAA AAATCACCAG TGAGGGGGGA 
GATGGTGCTA CCAAGTATAT CACCAAATCT GTAACCGTCA CTCAAAAGGT CGAAGAGCAT 
GAAGAGACCT TTGAGGAGAA ACTAGTGTCT ACTAAAAAGG TAGAGAAAGT CACTTCACAC 
GCCATAGTAA AGGAAGTCAC CCAGAGTGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806365.2
CDS	148..1800
Translation	MARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRQPSIAISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSVKEEVKEEEAEEKEEKEEAEEEVVAAKKSPVKATAPELKEEEGEKEEEEGQEEEEEEEEAAKSDQAEEGGSEKEGSSEKEEGEQEEEGETEAEGEGEEAAAEAKEEKKMEEKAEEVAPKEELAAEAKVEKPEKAKSPVAKSPTTKSPTAKSPEAKSPTAKSPTAKSPVAKSPTAKSPTAKSPEAKSPEAKSPTAKSPTAKSPAAKSPAAKSPVEEVKPKAEAGAEKGEQKEKVEEEKKEAKESPKEEKAEKKEEKAKDVPEKKKAESPVKAESPVKEEVPAKPVKVSPEKEAKEEEKPQEKEKEKEKVEEVGGKEEGGLKESRKEDIAINGEVEGKEEEQETKEKGSGGEEEKGVVTNGLDVSPGDEKKGGDKSEEKVVVTKMVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD

Target ORF information:

RefSeq Version	XM_010808063.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament medium (NEFM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808063.2

ORF Insert Sequence:

ATGGCTCGTC ATCTGCGAGA ATACCAGGAT CTCCTCAACG TCAAGATGGC TCTGGATATT 
GAGATCGCGG CGTACAGGAA ACTCCTGGAG GGTGAAGAGA CCAGATTTAG CACATTTGCA 
GGTAGCATCA CTGGGCCACT GTATACACAC CGACAGCCCT CCATCGCCAT ATCCAGTAAG 
ATTCAGAAAA CCAAGGTAGA GGCTCCCAAG CTAAAGGTCC AACACAAATT TGTTGAGGAG 
ATTATAGAGG AAACCAAGGT GGAAGATGAG AAATCAGAAA TGGAAGAAGC CCTGACGGCC 
ATTACCGAGG AATTGGCCGT TTCCGTGAAA GAGGAGGTCA AGGAAGAGGA GGCTGAAGAA 
AAGGAGGAGA AAGAAGAAGC CGAAGAAGAA GTTGTTGCTG CCAAAAAGTC TCCAGTGAAA 
GCTACTGCAC CTGAACTTAA AGAAGAGGAA GGAGAAAAGG AGGAGGAAGA GGGCCAAGAG 
GAAGAGGAAG AGGAAGAAGA GGCTGCTAAG TCAGACCAAG CCGAGGAAGG AGGATCTGAG 
AAGGAAGGTT CTAGTGAAAA AGAGGAAGGT GAGCAAGAAG AGGAAGGAGA AACAGAGGCT 
GAGGGGGAAG GAGAGGAAGC CGCTGCCGAA GCTAAGGAGG AAAAGAAAAT GGAGGAAAAG 
GCTGAAGAAG TGGCTCCAAA GGAGGAGCTG GCGGCAGAAG CCAAGGTGGA GAAGCCAGAG 
AAAGCCAAGT CCCCAGTGGC CAAGTCCCCA ACAACAAAGT CCCCAACGGC CAAGTCCCCA 
GAGGCAAAGT CCCCAACAGC AAAATCCCCG ACGGCCAAGT CCCCAGTGGC CAAGTCCCCG 
ACGGCCAAGT CCCCGACGGC CAAGTCCCCA GAGGCAAAGT CCCCAGAGGC AAAGTCCCCA 
ACAGCAAAAT CCCCGACGGC CAAGTCCCCA GCAGCAAAGT CCCCAGCGGC AAAATCACCT 
GTGGAGGAAG TGAAACCCAA AGCAGAAGCT GGAGCTGAGA AAGGAGAACA GAAGGAGAAG 
GTGGAGGAAG AAAAGAAAGA AGCAAAGGAA TCTCCCAAGG AAGAGAAGGC AGAGAAAAAG 
GAGGAGAAGG CAAAGGATGT GCCAGAGAAG AAGAAGGCTG AATCCCCAGT GAAGGCTGAG 
TCCCCAGTGA AGGAGGAGGT GCCTGCCAAG CCAGTAAAGG TGAGCCCAGA GAAGGAAGCC 
AAAGAGGAGG AGAAGCCACA GGAGAAAGAG AAGGAGAAGG AGAAAGTGGA AGAGGTGGGA 
GGGAAGGAGG AGGGAGGTTT GAAGGAATCC AGGAAGGAAG ACATAGCCAT CAATGGGGAG 
GTGGAAGGGA AGGAGGAAGA ACAGGAAACT AAGGAGAAAG GCAGTGGGGG AGAAGAGGAG 
AAAGGAGTCG TCACCAACGG GCTAGACGTG AGCCCAGGGG ATGAAAAGAA GGGCGGTGAT 
AAAAGTGAGG AGAAAGTGGT GGTAACCAAA ATGGTGGAAA AAATCACCAG TGAGGGGGGA 
GATGGTGCTA CCAAGTATAT CACCAAATCT GTAACCGTCA CTCAAAAGGT CGAAGAGCAT 
GAAGAGACCT TTGAGGAGAA ACTAGTGTCT ACTAAAAAGG TAGAGAAAGT CACTTCACAC 
GCCATAGTAA AGGAAGTCAC CCAGAGTGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFM ( NM_001244145.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001231074.1 Bos taurus neurofilament medium (NEFM), mRNA.

Price & Availability↑

CloneID	OBa44427
Clone ID Related Accession (Same CDS sequence)	NM_001244145.1
Accession Version	NM_001244145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Bos taurus(cattle)
Product	neurofilament medium polypeptide
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV839158.1, BC151624.1, DV831196.1 and EV613901.1. On or before Sep 14, 2011 this sequence version replaced XM_582135.4, XM_002689787.1. ##Evidence-Data-START## Transcript exon combination :: BC151624.1, DN521717.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145430 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTACA CGTTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT GACCGAAACC 
CGCTCGAGCT TCAGCCGCAT CAGCGGCTCC CCGTCCAGCG GCTTCCGCTC GCAGTCGTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT GGCCCCGCGC 
CTCACCTACA GCTCCGCCAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCTCTGC TTAACGGCGG TTCTGGGCCT GGAGGCGACT ACAAGCTGTC CCGCTCCAAC 
GAGAAGGAGC AGCTGCAGGG GCTGAACGAC CGCTTCGCGG GCTACATCGA GAAGGTCCAC 
TACCTGGAGC AGCAGAACAA GGAGATCGAG GCAGAGATCC AGGCGCTGCG GCAGAAGCAG 
GCCTCGCACG CCCAGCTGGG CGACGCGTAC GACCAGGAAA TCCGCGAGCT ACGCGCCACC 
CTGGAGATGG TGAACCATGA GAAGGCTCAG GTACAGCTGG ACTCGGACCA CCTGGAAGAG 
GATATCCACC GGCTCAAGGA GCGCTTCGAG GAGGAGGCAC GGCTGCGCGA CGACACCGAG 
GCGGCTATCC GCGCGCTGCG CAAAGATATC GAGGAGTCGT CGCTGGTCAA GGTGGAGCTG 
GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA TCACGAGGAG 
GAGGTGGCCG ACCTGCTGGC CCAGATCCAA GCGTCACACA TCACCGTGGA GCGCAAAGAC 
TACCTGAAGA CGGACATCTC GACGGCGCTG AAAGAGATCC GCTCCCAGCT CGAGAGTCAC 
TCCGACCAGA ACATGCACCA GGCCGAAGAG TGGTTTAAGT GCCGCTACGC CAAGCTCACC 
GAGGCGGCCG AGCAGAACAA GGAAGCCATC CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC 
CGGCGCCAGC TGCAGTCCAA GAGCATCGAG CTCGAGTCAG TGCGCGGCAC CAAGGAGTCC 
CTGGAGCGGC AGCTCAGCGA CATCGAGGAG CGCCACAACC ACGACCTTAG CAGCTACCAG 
GACACCATCC AGCAGCTGGA AAATGAGCTT CGGGGCACAA AGTGGGAAAT GGCTCGTCAT 
CTGCGAGAAT ACCAGGATCT CCTCAACGTC AAGATGGCTC TGGATATTGA GATCGCGGCG 
TACAGGAAAC TCCTGGAGGG TGAAGAGACC AGATTTAGCA CATTTGCAGG TAGCATCACT 
GGGCCACTGT ATACACACCG ACAGCCCTCC ATCGCCATAT CCAGTAAGAT TCAGAAAACC 
AAGGTAGAGG CTCCCAAGCT AAAGGTCCAA CACAAATTTG TTGAGGAGAT TATAGAGGAA 
ACCAAGGTGG AAGATGAGAA ATCAGAAATG GAAGAAGCCC TGACGGCCAT TACCGAGGAA 
TTGGCCGTTT CCGTGAAAGA GGAGGTCAAG GAAGAGGAGG CTGAAGAAAA GGAGGAGAAA 
GAAGAAGCCG AAGAAGAAGT TGTTGCTGCC AAAAAGTCTC CAGTGAAAGC TACTGCACCT 
GAACTTAAAG AAGAGGAAGG AGAAAAGGAG GAGGAAGAGG GCCAAGAGGA AGAGGAAGAG 
GAAGAAGAGG CTGCTAAGTC AGACCAAGCC GAGGAAGGAG GATCTGAGAA GGAAGGTTCT 
AGTGAAAAAG AGGAAGGTGA GCAAGAAGAG GAAGGAGAAA CAGAGGCTGA GGGGGAAGGA 
GAGGAAGCCG CTGCCGAAGC TAAGGAGGAA AAGAAAATGG AGGAAAAGGC TGAAGAAGTG 
GCTCCAAAGG AGGAGCTGGC GGCAGAAGCC AAGGTGGAGA AGCCAGAGAA AGCCAAGTCC 
CCAGTGGCCA AGTCCCCAAC AACAAAGTCC CCAACGGCCA AGTCCCCAGA GGCAAAGTCC 
CCAGAGGCAA AGTCCCCAAC AGCAAAATCC CCGACGGCCA AGTCCCCAGT GGCCAAGTCC 
CCGACGGCCA AGTCCCCGAC GGCCAAGTCC CCAGAGGCAA AGTCCCCAGA GGCAAAGTCC 
CCAACAGCAA AATCCCCGAC GGCCAAGTCC CCAGCAGCAA AGTCCCCAGC GGCAAAATCA 
CCTGTGGAGG AAGTGAAACC CAAAGCAGAA GCTGGAGCTG AGAAAGGAGA ACAGAAGGAG 
AAGGTGGAGG AAGAAAAGAA AGAAGCAAAG GAATCTCCCA AGGAAGAGAA GGCAGAGAAA 
AAGGAGGAGA AGGCAAAGGA TGTGCCAGAG AAGAAGAAGG CTGAATCCCC AGTGAAGGCT 
GAGTCCCCAG TGAAGGAGGA GGTGCCTGCC AAGCCAGTAA AGGTGAGCCC AGAGAAGGAA 
GCCAAAGAGG AGGAGAAGCC ACAGGAGAAA GAGAAGGAGA AGGAGAAAGT GGAAGAGGTG 
GGAGGGAAGG AGGAGGGAGG TTTGAAGGAA TCCAGGAAGG AAGACATAGC CATCAATGGG 
GAGGTGGAAG GGAAGGAGGA AGAACAGGAA ACTAAGGAGA AAGGCAGTGG GGGAGAAGAG 
GAGAAAGGAG TCGTCACCAA CGGGCTAGAC GTGAGCCCAG GGGATGAAAA GAAGGGCGGT 
GATAAAAGTG AGGAGAAAGT GGTGGTAACC AAAATGGTGG AAAAAATCAC CAGTGAGGGG 
GGAGATGGTG CTACCAAGTA TATCACCAAA TCTGTAACCG TCACTCAAAA GGTCGAAGAG 
CATGAAGAGA CCTTTGAGGA GAAACTAGTG TCTACTAAAA AGGTAGAGAA AGTCACTTCA 
CACGCCATAG TAAAGGAAGT CACCCAGAGT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231074.1
CDS	45..2840
Translation	MSYTLDSLGNPSAYRRVTETRSSFSRISGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLTYSSAMLSSAESSLDFSQSSSLLNGGSGPGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEESSLVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRQPSIAISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSVKEEVKEEEAEEKEEKEEAEEEVVAAKKSPVKATAPELKEEEGEKEEEEGQEEEEEEEEAAKSDQAEEGGSEKEGSSEKEEGEQEEEGETEAEGEGEEAAAEAKEEKKMEEKAEEVAPKEELAAEAKVEKPEKAKSPVAKSPTTKSPTAKSPEAKSPEAKSPTAKSPTAKSPVAKSPTAKSPTAKSPEAKSPEAKSPTAKSPTAKSPAAKSPAAKSPVEEVKPKAEAGAEKGEQKEKVEEEKKEAKESPKEEKAEKKEEKAKDVPEKKKAESPVKAESPVKEEVPAKPVKVSPEKEAKEEEKPQEKEKEKEKVEEVGGKEEGGLKESRKEDIAINGEVEGKEEEQETKEKGSGGEEEKGVVTNGLDVSPGDEKKGGDKSEEKVVVTKMVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD

Target ORF information:

RefSeq Version	NM_001244145.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament medium (NEFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244145.1

ORF Insert Sequence:

ATGAGCTACA CGTTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT GACCGAAACC 
CGCTCGAGCT TCAGCCGCAT CAGCGGCTCC CCGTCCAGCG GCTTCCGCTC GCAGTCGTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT GGCCCCGCGC 
CTCACCTACA GCTCCGCCAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCTCTGC TTAACGGCGG TTCTGGGCCT GGAGGCGACT ACAAGCTGTC CCGCTCCAAC 
GAGAAGGAGC AGCTGCAGGG GCTGAACGAC CGCTTCGCGG GCTACATCGA GAAGGTCCAC 
TACCTGGAGC AGCAGAACAA GGAGATCGAG GCAGAGATCC AGGCGCTGCG GCAGAAGCAG 
GCCTCGCACG CCCAGCTGGG CGACGCGTAC GACCAGGAAA TCCGCGAGCT ACGCGCCACC 
CTGGAGATGG TGAACCATGA GAAGGCTCAG GTACAGCTGG ACTCGGACCA CCTGGAAGAG 
GATATCCACC GGCTCAAGGA GCGCTTCGAG GAGGAGGCAC GGCTGCGCGA CGACACCGAG 
GCGGCTATCC GCGCGCTGCG CAAAGATATC GAGGAGTCGT CGCTGGTCAA GGTGGAGCTG 
GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA TCACGAGGAG 
GAGGTGGCCG ACCTGCTGGC CCAGATCCAA GCGTCACACA TCACCGTGGA GCGCAAAGAC 
TACCTGAAGA CGGACATCTC GACGGCGCTG AAAGAGATCC GCTCCCAGCT CGAGAGTCAC 
TCCGACCAGA ACATGCACCA GGCCGAAGAG TGGTTTAAGT GCCGCTACGC CAAGCTCACC 
GAGGCGGCCG AGCAGAACAA GGAAGCCATC CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC 
CGGCGCCAGC TGCAGTCCAA GAGCATCGAG CTCGAGTCAG TGCGCGGCAC CAAGGAGTCC 
CTGGAGCGGC AGCTCAGCGA CATCGAGGAG CGCCACAACC ACGACCTTAG CAGCTACCAG 
GACACCATCC AGCAGCTGGA AAATGAGCTT CGGGGCACAA AGTGGGAAAT GGCTCGTCAT 
CTGCGAGAAT ACCAGGATCT CCTCAACGTC AAGATGGCTC TGGATATTGA GATCGCGGCG 
TACAGGAAAC TCCTGGAGGG TGAAGAGACC AGATTTAGCA CATTTGCAGG TAGCATCACT 
GGGCCACTGT ATACACACCG ACAGCCCTCC ATCGCCATAT CCAGTAAGAT TCAGAAAACC 
AAGGTAGAGG CTCCCAAGCT AAAGGTCCAA CACAAATTTG TTGAGGAGAT TATAGAGGAA 
ACCAAGGTGG AAGATGAGAA ATCAGAAATG GAAGAAGCCC TGACGGCCAT TACCGAGGAA 
TTGGCCGTTT CCGTGAAAGA GGAGGTCAAG GAAGAGGAGG CTGAAGAAAA GGAGGAGAAA 
GAAGAAGCCG AAGAAGAAGT TGTTGCTGCC AAAAAGTCTC CAGTGAAAGC TACTGCACCT 
GAACTTAAAG AAGAGGAAGG AGAAAAGGAG GAGGAAGAGG GCCAAGAGGA AGAGGAAGAG 
GAAGAAGAGG CTGCTAAGTC AGACCAAGCC GAGGAAGGAG GATCTGAGAA GGAAGGTTCT 
AGTGAAAAAG AGGAAGGTGA GCAAGAAGAG GAAGGAGAAA CAGAGGCTGA GGGGGAAGGA 
GAGGAAGCCG CTGCCGAAGC TAAGGAGGAA AAGAAAATGG AGGAAAAGGC TGAAGAAGTG 
GCTCCAAAGG AGGAGCTGGC GGCAGAAGCC AAGGTGGAGA AGCCAGAGAA AGCCAAGTCC 
CCAGTGGCCA AGTCCCCAAC AACAAAGTCC CCAACGGCCA AGTCCCCAGA GGCAAAGTCC 
CCAGAGGCAA AGTCCCCAAC AGCAAAATCC CCGACGGCCA AGTCCCCAGT GGCCAAGTCC 
CCGACGGCCA AGTCCCCGAC GGCCAAGTCC CCAGAGGCAA AGTCCCCAGA GGCAAAGTCC 
CCAACAGCAA AATCCCCGAC GGCCAAGTCC CCAGCAGCAA AGTCCCCAGC GGCAAAATCA 
CCTGTGGAGG AAGTGAAACC CAAAGCAGAA GCTGGAGCTG AGAAAGGAGA ACAGAAGGAG 
AAGGTGGAGG AAGAAAAGAA AGAAGCAAAG GAATCTCCCA AGGAAGAGAA GGCAGAGAAA 
AAGGAGGAGA AGGCAAAGGA TGTGCCAGAG AAGAAGAAGG CTGAATCCCC AGTGAAGGCT 
GAGTCCCCAG TGAAGGAGGA GGTGCCTGCC AAGCCAGTAA AGGTGAGCCC AGAGAAGGAA 
GCCAAAGAGG AGGAGAAGCC ACAGGAGAAA GAGAAGGAGA AGGAGAAAGT GGAAGAGGTG 
GGAGGGAAGG AGGAGGGAGG TTTGAAGGAA TCCAGGAAGG AAGACATAGC CATCAATGGG 
GAGGTGGAAG GGAAGGAGGA AGAACAGGAA ACTAAGGAGA AAGGCAGTGG GGGAGAAGAG 
GAGAAAGGAG TCGTCACCAA CGGGCTAGAC GTGAGCCCAG GGGATGAAAA GAAGGGCGGT 
GATAAAAGTG AGGAGAAAGT GGTGGTAACC AAAATGGTGG AAAAAATCAC CAGTGAGGGG 
GGAGATGGTG CTACCAAGTA TATCACCAAA TCTGTAACCG TCACTCAAAA GGTCGAAGAG 
CATGAAGAGA CCTTTGAGGA GAAACTAGTG TCTACTAAAA AGGTAGAGAA AGTCACTTCA 
CACGCCATAG TAAAGGAAGT CACCCAGAGT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFM ( XM_005210283.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210340.1 Bos taurus neurofilament, medium polypeptide (NEFM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa22185
Clone ID Related Accession (Same CDS sequence)	XM_005210283.3
Accession Version	XM_005210283.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament medium polypeptide isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210283.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACA CGTTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT GACCGAAACC 
CGCTCGAGCT TCAGCCGCAT CAGCGGCTCC CCGTCCAGCG GCTTCCGCTC GCAGTCGTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT GGCCCCGCGC 
CTCACCTACA GCTCCGCCAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCTCTGC TTAACGGCGG TTCTGGGCCT GGAGGCGACT ACAAGCTGTC CCGCTCCAAC 
GAGAAGGAGC AGCTGCAGGG GCTGAACGAC CGCTTCGCGG GCTACATCGA GAAGGTCCAC 
TACCTGGAGC AGCAGAACAA GGAGATCGAG GCAGAGATCC AGGCGCTGCG GCAGAAGCAG 
GCCTCGCACG CCCAGCTGGG CGACGCGTAC GACCAGGAAA TCCGCGAGCT ACGCGCCACC 
CTGGAGATGG TGAACCATGA GAAGGCTCAG GTACAGCTGG ACTCGGACCA CCTGGAAGAG 
GATATCCACC GGCTCAAGGA GCGCTTCGAG GAGGAGGCAC GGCTGCGCGA CGACACCGAG 
GCGGCTATCC GCGCGCTGCG CAAAGATATC GAGGAGTCGT CGCTGGTCAA GGTGGAGCTG 
GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA TCACGAGGAG 
GAGGTGGCCG ACCTGCTGGC CCAGATCCAA GCGTCACACA TCACCGTGGA GCGCAAAGAC 
TACCTGAAGA CGGACATCTC GACGGCGCTG AAAGAGATCC GCTCCCAGCT CGAGAGTCAC 
TCCGACCAGA ACATGCACCA GGCCGAAGAG TGGTTTAAGT GCCGCTACGC CAAGCTCACC 
GAGGCGGCCG AGCAGAACAA GGAAGCCATC CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC 
CGGCGCCAGC TGCAGTCCAA GAGCATCGAG CTCGAGTCAG TGCGCGGCAC CAAGGAGTCC 
CTGGAGCGGC AGCTCAGCGA CATCGAGGAG CGCCACAACC ACGACCTTAG CAGCTACCAG 
GACACCATCC AGCAGCTGGA AAATGAGCTT CGGGGCACAA AGTGGGAAAT GGCTCGTCAT 
CTGCGAGAAT ACCAGGATCT CCTCAACGTC AAGATGGCTC TGGATATTGA GATCGCGGCG 
TACAGGAAAC TCCTGGAGGG TGAAGAGACC AGATTTAGCA CATTTGCAGG TAGCATCACT 
GGGCCACTGT ATACACACCG ACAGCCCTCC ATCGCCATAT CCAGTAAGAT TCAGAAAACC 
AAGGTAGAGG CTCCCAAGCT AAAGGTCCAA CACAAATTTG TTGAGGAGAT TATAGAGGAA 
ACCAAGGTGG AAGATGAGAA ATCAGAAATG GAAGAAGCCC TGACGGCCAT TACCGAGGAA 
TTGGCCGTTT CCGTGAAAGA GGAGGTCAAG GAAGAGGAGG CTGAAGAAAA GGAGGAGAAA 
GAAGAAGCCG AAGAAGAAGT TGTTGCTGCC AAAAAGTCTC CAGTGAAAGC TACTGCACCT 
GAACTTAAAG AAGAGGAAGG AGAAAAGGAG GAGGAAGAGG GCCAAGAGGA AGAGGAAGAG 
GAAGAAGAGG CTGCTAAGTC AGACCAAGCC GAGGAAGGAG GATCTGAGAA GGAAGGTTCT 
AGTGAAAAAG AGGAAGGTGA GCAAGAAGAG GAAGGAGAAA CAGAGGCTGA GGGGGAAGGA 
GAGGAAGCCG CTGCCGAAGC TAAGGAGGAA AAGAAAATGG AGGAAAAGGC TGAAGAAGTG 
GCTCCAAAGG AGGAGCTGGC GGCAGAAGCC AAGGTGGAGA AGCCAGAGAA AGCCAAGTCC 
CCAGTGGCCA AGTCCCCAAC AACAAAGTCC CCAACGGCCA AGTCCCCAGA GGCAAAGTCC 
CCAACAGCAA AATCCCCGAC GGCCAAGTCC CCAGCAGCAA AGTCCCCAGC GGCAAAATCA 
CCTGTGGAGG AAGTGAAACC CAAAGCAGAA GCTGGAGCTG AGAAAGGAGA ACAGAAGGAG 
AAGGTGGAGG AAGAAAAGAA AGAAGCAAAG GAATCTCCCA AGGAAGAGAA GGCAGAGAAA 
AAGGAGGAGA AGGCAAAGGA TGTGCCAGAG AAGAAGAAGG CTGAATCCCC AGTGAAGGCT 
GAGTCCCCAG TGAAGGAGGA GGTGCCTGCC AAGCCAGTAA AGGTGAGCCC AGAGAAGGAA 
GCCAAAGAGG AGGAGAAGCC ACAGGAGAAA GAGAAGGAGA AGGAGAAAGT GGAAGAGGTG 
GGAGGGAAGG AGGAGGGAGG TTTGAAGGAA TCCAGGAAGG AAGACATAGC CATCAATGGG 
GAGGTGGAAG GGAAGGAGGA AGAACAGGAA ACTAAGGAGA AAGGCAGTGG GGGAGAAGAG 
GAGAAAGGAG TCGTCACCAA CGGGCTAGAC GTGAGCCCAG GGGATGAAAA GAAGGGCGGT 
GATAAAAGTG AGGAGAAAGT GGTGGTAACC AAAATGGTGG AAAAAATCAC CAGTGAGGGG 
GGAGATGGTG CTACCAAGTA TATCACCAAA TCTGTAACCG TCACTCAAAA GGTCGAAGAG 
CATGAAGAGA CCTTTGAGGA GAAACTAGTG TCTACTAAAA AGGTAGAGAA AGTCACTTCA 
CACGCCATAG TAAAGGAAGT CACCCAGAGT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210340.1
CDS	6397..9072
Translation	MSYTLDSLGNPSAYRRVTETRSSFSRISGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLTYSSAMLSSAESSLDFSQSSSLLNGGSGPGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEESSLVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRQPSIAISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSVKEEVKEEEAEEKEEKEEAEEEVVAAKKSPVKATAPELKEEEGEKEEEEGQEEEEEEEEAAKSDQAEEGGSEKEGSSEKEEGEQEEEGETEAEGEGEEAAAEAKEEKKMEEKAEEVAPKEELAAEAKVEKPEKAKSPVAKSPTTKSPTAKSPEAKSPTAKSPTAKSPAAKSPAAKSPVEEVKPKAEAGAEKGEQKEKVEEEKKEAKESPKEEKAEKKEEKAKDVPEKKKAESPVKAESPVKEEVPAKPVKVSPEKEAKEEEKPQEKEKEKEKVEEVGGKEEGGLKESRKEDIAINGEVEGKEEEQETKEKGSGGEEEKGVVTNGLDVSPGDEKKGGDKSEEKVVVTKMVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD

Target ORF information:

RefSeq Version	XM_005210283.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, medium polypeptide (NEFM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210283.3

ORF Insert Sequence:

ATGAGCTACA CGTTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT GACCGAAACC 
CGCTCGAGCT TCAGCCGCAT CAGCGGCTCC CCGTCCAGCG GCTTCCGCTC GCAGTCGTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT GGCCCCGCGC 
CTCACCTACA GCTCCGCCAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCTCTGC TTAACGGCGG TTCTGGGCCT GGAGGCGACT ACAAGCTGTC CCGCTCCAAC 
GAGAAGGAGC AGCTGCAGGG GCTGAACGAC CGCTTCGCGG GCTACATCGA GAAGGTCCAC 
TACCTGGAGC AGCAGAACAA GGAGATCGAG GCAGAGATCC AGGCGCTGCG GCAGAAGCAG 
GCCTCGCACG CCCAGCTGGG CGACGCGTAC GACCAGGAAA TCCGCGAGCT ACGCGCCACC 
CTGGAGATGG TGAACCATGA GAAGGCTCAG GTACAGCTGG ACTCGGACCA CCTGGAAGAG 
GATATCCACC GGCTCAAGGA GCGCTTCGAG GAGGAGGCAC GGCTGCGCGA CGACACCGAG 
GCGGCTATCC GCGCGCTGCG CAAAGATATC GAGGAGTCGT CGCTGGTCAA GGTGGAGCTG 
GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA TCACGAGGAG 
GAGGTGGCCG ACCTGCTGGC CCAGATCCAA GCGTCACACA TCACCGTGGA GCGCAAAGAC 
TACCTGAAGA CGGACATCTC GACGGCGCTG AAAGAGATCC GCTCCCAGCT CGAGAGTCAC 
TCCGACCAGA ACATGCACCA GGCCGAAGAG TGGTTTAAGT GCCGCTACGC CAAGCTCACC 
GAGGCGGCCG AGCAGAACAA GGAAGCCATC CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC 
CGGCGCCAGC TGCAGTCCAA GAGCATCGAG CTCGAGTCAG TGCGCGGCAC CAAGGAGTCC 
CTGGAGCGGC AGCTCAGCGA CATCGAGGAG CGCCACAACC ACGACCTTAG CAGCTACCAG 
GACACCATCC AGCAGCTGGA AAATGAGCTT CGGGGCACAA AGTGGGAAAT GGCTCGTCAT 
CTGCGAGAAT ACCAGGATCT CCTCAACGTC AAGATGGCTC TGGATATTGA GATCGCGGCG 
TACAGGAAAC TCCTGGAGGG TGAAGAGACC AGATTTAGCA CATTTGCAGG TAGCATCACT 
GGGCCACTGT ATACACACCG ACAGCCCTCC ATCGCCATAT CCAGTAAGAT TCAGAAAACC 
AAGGTAGAGG CTCCCAAGCT AAAGGTCCAA CACAAATTTG TTGAGGAGAT TATAGAGGAA 
ACCAAGGTGG AAGATGAGAA ATCAGAAATG GAAGAAGCCC TGACGGCCAT TACCGAGGAA 
TTGGCCGTTT CCGTGAAAGA GGAGGTCAAG GAAGAGGAGG CTGAAGAAAA GGAGGAGAAA 
GAAGAAGCCG AAGAAGAAGT TGTTGCTGCC AAAAAGTCTC CAGTGAAAGC TACTGCACCT 
GAACTTAAAG AAGAGGAAGG AGAAAAGGAG GAGGAAGAGG GCCAAGAGGA AGAGGAAGAG 
GAAGAAGAGG CTGCTAAGTC AGACCAAGCC GAGGAAGGAG GATCTGAGAA GGAAGGTTCT 
AGTGAAAAAG AGGAAGGTGA GCAAGAAGAG GAAGGAGAAA CAGAGGCTGA GGGGGAAGGA 
GAGGAAGCCG CTGCCGAAGC TAAGGAGGAA AAGAAAATGG AGGAAAAGGC TGAAGAAGTG 
GCTCCAAAGG AGGAGCTGGC GGCAGAAGCC AAGGTGGAGA AGCCAGAGAA AGCCAAGTCC 
CCAGTGGCCA AGTCCCCAAC AACAAAGTCC CCAACGGCCA AGTCCCCAGA GGCAAAGTCC 
CCAACAGCAA AATCCCCGAC GGCCAAGTCC CCAGCAGCAA AGTCCCCAGC GGCAAAATCA 
CCTGTGGAGG AAGTGAAACC CAAAGCAGAA GCTGGAGCTG AGAAAGGAGA ACAGAAGGAG 
AAGGTGGAGG AAGAAAAGAA AGAAGCAAAG GAATCTCCCA AGGAAGAGAA GGCAGAGAAA 
AAGGAGGAGA AGGCAAAGGA TGTGCCAGAG AAGAAGAAGG CTGAATCCCC AGTGAAGGCT 
GAGTCCCCAG TGAAGGAGGA GGTGCCTGCC AAGCCAGTAA AGGTGAGCCC AGAGAAGGAA 
GCCAAAGAGG AGGAGAAGCC ACAGGAGAAA GAGAAGGAGA AGGAGAAAGT GGAAGAGGTG 
GGAGGGAAGG AGGAGGGAGG TTTGAAGGAA TCCAGGAAGG AAGACATAGC CATCAATGGG 
GAGGTGGAAG GGAAGGAGGA AGAACAGGAA ACTAAGGAGA AAGGCAGTGG GGGAGAAGAG 
GAGAAAGGAG TCGTCACCAA CGGGCTAGAC GTGAGCCCAG GGGATGAAAA GAAGGGCGGT 
GATAAAAGTG AGGAGAAAGT GGTGGTAACC AAAATGGTGG AAAAAATCAC CAGTGAGGGG 
GGAGATGGTG CTACCAAGTA TATCACCAAA TCTGTAACCG TCACTCAAAA GGTCGAAGAG 
CATGAAGAGA CCTTTGAGGA GAAACTAGTG TCTACTAAAA AGGTAGAGAA AGTCACTTCA 
CACGCCATAG TAAAGGAAGT CACCCAGAGT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFM ( XM_010808063.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806365.1 Bos taurus neurofilament, medium polypeptide (NEFM), transcript variant X2, mRNA.

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CloneID	OBa45125
Clone ID Related Accession (Same CDS sequence)	XM_010808063.1
Accession Version	XM_010808063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neurofilament medium polypeptide isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGTC ATCTGCGAGA ATACCAGGAT CTCCTCAACG TCAAGATGGC TCTGGATATT 
GAGATCGCGG CGTACAGGAA ACTCCTGGAG GGTGAAGAGA CCAGATTTAG CACATTTGCA 
GGTAGCATCA CTGGGCCACT GTATACACAC CGACAGCCCT CCATCGCCAT ATCCAGTAAG 
ATTCAGAAAA CCAAGGTAGA GGCTCCCAAG CTAAAGGTCC AACACAAATT TGTTGAGGAG 
ATTATAGAGG AAACCAAGGT GGAAGATGAG AAATCAGAAA TGGAAGAAGC CCTGACGGCC 
ATTACCGAGG AATTGGCCGT TTCCGTGAAA GAGGAGGTCA AGGAAGAGGA GGCTGAAGAA 
AAGGAGGAGA AAGAAGAAGC CGAAGAAGAA GTTGTTGCTG CCAAAAAGTC TCCAGTGAAA 
GCTACTGCAC CTGAACTTAA AGAAGAGGAA GGAGAAAAGG AGGAGGAAGA GGGCCAAGAG 
GAAGAGGAAG AGGAAGAAGA GGCTGCTAAG TCAGACCAAG CCGAGGAAGG AGGATCTGAG 
AAGGAAGGTT CTAGTGAAAA AGAGGAAGGT GAGCAAGAAG AGGAAGGAGA AACAGAGGCT 
GAGGGGGAAG GAGAGGAAGC CGCTGCCGAA GCTAAGGAGG AAAAGAAAAT GGAGGAAAAG 
GCTGAAGAAG TGGCTCCAAA GGAGGAGCTG GCGGCAGAAG CCAAGGTGGA GAAGCCAGAG 
AAAGCCAAGT CCCCAGTGGC CAAGTCCCCA ACAACAAAGT CCCCAACGGC CAAGTCCCCA 
GAGGCAAAGT CCCCAGAGGC AAAGTCCCCA ACAGCAAAAT CCCCGACGGC CAAGTCCCCA 
GTGGCCAAGT CCCCGACGGC CAAGTCCCCG ACGGCCAAGT CCCCAGAGGC AAAGTCCCCA 
GAGGCAAAGT CCCCAACAGC AAAATCCCCG ACGGCCAAGT CCCCAGCAGC AAAGTCCCCA 
GCGGCAAAAT CACCTGTGGA GGAAGTGAAA CCCAAAGCAG AAGCTGGAGC TGAGAAAGGA 
GAACAGAAGG AGAAGGTGGA GGAAGAAAAG AAAGAAGCAA AGGAATCTCC CAAGGAAGAG 
AAGGCAGAGA AAAAGGAGGA GAAGGCAAAG GATGTGCCAG AGAAGAAGAA GGCTGAATCC 
CCAGTGAAGG CTGAGTCCCC AGTGAAGGAG GAGGTGCCTG CCAAGCCAGT AAAGGTGAGC 
CCAGAGAAGG AAGCCAAAGA GGAGGAGAAG CCACAGGAGA AAGAGAAGGA GAAGGAGAAA 
GTGGAAGAGG TGGGAGGGAA GGAGGAGGGA GGTTTGAAGG AATCCAGGAA GGAAGACATA 
GCCATCAATG GGGAGGTGGA AGGGAAGGAG GAAGAACAGG AAACTAAGGA GAAAGGCAGT 
GGGGGAGAAG AGGAGAAAGG AGTCGTCACC AACGGGCTAG ACGTGAGCCC AGGGGATGAA 
AAGAAGGGCG GTGATAAAAG TGAGGAGAAA GTGGTGGTAA CCAAAATGGT GGAAAAAATC 
ACCAGTGAGG GGGGAGATGG TGCTACCAAG TATATCACCA AATCTGTAAC CGTCACTCAA 
AAGGTCGAAG AGCATGAAGA GACCTTTGAG GAGAAACTAG TGTCTACTAA AAAGGTAGAG 
AAAGTCACTT CACACGCCAT AGTAAAGGAA GTCACCCAGA GTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806365.1
CDS	155..1822
Translation	MARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRQPSIAISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSVKEEVKEEEAEEKEEKEEAEEEVVAAKKSPVKATAPELKEEEGEKEEEEGQEEEEEEEEAAKSDQAEEGGSEKEGSSEKEEGEQEEEGETEAEGEGEEAAAEAKEEKKMEEKAEEVAPKEELAAEAKVEKPEKAKSPVAKSPTTKSPTAKSPEAKSPEAKSPTAKSPTAKSPVAKSPTAKSPTAKSPEAKSPEAKSPTAKSPTAKSPAAKSPAAKSPVEEVKPKAEAGAEKGEQKEKVEEEKKEAKESPKEEKAEKKEEKAKDVPEKKKAESPVKAESPVKEEVPAKPVKVSPEKEAKEEEKPQEKEKEKEKVEEVGGKEEGGLKESRKEDIAINGEVEGKEEEQETKEKGSGGEEEKGVVTNGLDVSPGDEKKGGDKSEEKVVVTKMVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD

Target ORF information:

RefSeq Version	XM_010808063.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament, medium polypeptide (NEFM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808063.1

ORF Insert Sequence:

ATGGCTCGTC ATCTGCGAGA ATACCAGGAT CTCCTCAACG TCAAGATGGC TCTGGATATT 
GAGATCGCGG CGTACAGGAA ACTCCTGGAG GGTGAAGAGA CCAGATTTAG CACATTTGCA 
GGTAGCATCA CTGGGCCACT GTATACACAC CGACAGCCCT CCATCGCCAT ATCCAGTAAG 
ATTCAGAAAA CCAAGGTAGA GGCTCCCAAG CTAAAGGTCC AACACAAATT TGTTGAGGAG 
ATTATAGAGG AAACCAAGGT GGAAGATGAG AAATCAGAAA TGGAAGAAGC CCTGACGGCC 
ATTACCGAGG AATTGGCCGT TTCCGTGAAA GAGGAGGTCA AGGAAGAGGA GGCTGAAGAA 
AAGGAGGAGA AAGAAGAAGC CGAAGAAGAA GTTGTTGCTG CCAAAAAGTC TCCAGTGAAA 
GCTACTGCAC CTGAACTTAA AGAAGAGGAA GGAGAAAAGG AGGAGGAAGA GGGCCAAGAG 
GAAGAGGAAG AGGAAGAAGA GGCTGCTAAG TCAGACCAAG CCGAGGAAGG AGGATCTGAG 
AAGGAAGGTT CTAGTGAAAA AGAGGAAGGT GAGCAAGAAG AGGAAGGAGA AACAGAGGCT 
GAGGGGGAAG GAGAGGAAGC CGCTGCCGAA GCTAAGGAGG AAAAGAAAAT GGAGGAAAAG 
GCTGAAGAAG TGGCTCCAAA GGAGGAGCTG GCGGCAGAAG CCAAGGTGGA GAAGCCAGAG 
AAAGCCAAGT CCCCAGTGGC CAAGTCCCCA ACAACAAAGT CCCCAACGGC CAAGTCCCCA 
GAGGCAAAGT CCCCAGAGGC AAAGTCCCCA ACAGCAAAAT CCCCGACGGC CAAGTCCCCA 
GTGGCCAAGT CCCCGACGGC CAAGTCCCCG ACGGCCAAGT CCCCAGAGGC AAAGTCCCCA 
GAGGCAAAGT CCCCAACAGC AAAATCCCCG ACGGCCAAGT CCCCAGCAGC AAAGTCCCCA 
GCGGCAAAAT CACCTGTGGA GGAAGTGAAA CCCAAAGCAG AAGCTGGAGC TGAGAAAGGA 
GAACAGAAGG AGAAGGTGGA GGAAGAAAAG AAAGAAGCAA AGGAATCTCC CAAGGAAGAG 
AAGGCAGAGA AAAAGGAGGA GAAGGCAAAG GATGTGCCAG AGAAGAAGAA GGCTGAATCC 
CCAGTGAAGG CTGAGTCCCC AGTGAAGGAG GAGGTGCCTG CCAAGCCAGT AAAGGTGAGC 
CCAGAGAAGG AAGCCAAAGA GGAGGAGAAG CCACAGGAGA AAGAGAAGGA GAAGGAGAAA 
GTGGAAGAGG TGGGAGGGAA GGAGGAGGGA GGTTTGAAGG AATCCAGGAA GGAAGACATA 
GCCATCAATG GGGAGGTGGA AGGGAAGGAG GAAGAACAGG AAACTAAGGA GAAAGGCAGT 
GGGGGAGAAG AGGAGAAAGG AGTCGTCACC AACGGGCTAG ACGTGAGCCC AGGGGATGAA 
AAGAAGGGCG GTGATAAAAG TGAGGAGAAA GTGGTGGTAA CCAAAATGGT GGAAAAAATC 
ACCAGTGAGG GGGGAGATGG TGCTACCAAG TATATCACCA AATCTGTAAC CGTCACTCAA 
AAGGTCGAAG AGCATGAAGA GACCTTTGAG GAGAAACTAG TGTCTACTAA AAAGGTAGAG 
AAAGTCACTT CACACGCCAT AGTAAAGGAA GTCACCCAGA GTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFM ( XM_024995441.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851209.1 Bos taurus neurofilament medium (NEFM), transcript variant X1, mRNA.

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CloneID	OBa61918
Clone ID Related Accession (Same CDS sequence)	XM_024995441.1
Accession Version	XM_024995441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neurofilament medium polypeptide isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACA CGTTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT GACCGAAACC 
CGCTCGAGCT TCAGCCGCAT CAGCGGCTCC CCGTCCAGCG GCTTCCGCTC GCAGTCGTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT GGCCCCGCGC 
CTCACCTACA GCTCCGCCAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCTCTGC TTAACGGCGG TTCTGGGCCT GGAGGCGACT ACAAGCTGTC CCGCTCCAAC 
GAGAAGGAGC AGCTGCAGGG GCTGAACGAC CGCTTCGCGG GCTACATCGA GAAGGTCCAC 
TACCTGGAGC AGCAGAACAA GGAGATCGAG GCAGAGATCC AGGCGCTGCG GCAGAAGCAG 
GCCTCGCACG CCCAGCTGGG CGACGCGTAC GACCAGGAAA TCCGCGAGCT ACGCGCCACC 
CTGGAGATGG TGAACCATGA GAAGGCTCAG GTACAGCTGG ACTCGGACCA CCTGGAAGAG 
GATATCCACC GGCTCAAGGA GCGCTTCGAG GAGGAGGCAC GGCTGCGCGA CGACACCGAG 
GCGGCTATCC GCGCGCTGCG CAAAGATATC GAGGAGTCGT CGCTGGTCAA GGTGGAGCTG 
GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA TCACGAGGAG 
GAGGTGGCCG ACCTGCTGGC CCAGATCCAA GCGTCACACA TCACCGTGGA GCGCAAAGAC 
TACCTGAAGA CGGACATCTC GACGGCGCTG AAAGAGATCC GCTCCCAGCT CGAGAGTCAC 
TCCGACCAGA ACATGCACCA GGCCGAAGAG TGGTTTAAGT GCCGTTACGC CAAGCTCACC 
GAGGCGGCCG AGCAGAACAA GGAAGCCATC CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC 
CGGCGCCAGC TGCAGTCCAA GAGCATCGAG CTCGAGTCAG TGCGCGGCAC CAAGGAGTCC 
CTGGAGCGGC AGCTCAGCGA CATCGAGGAG CGCCACAACC ACGACCTTAG CAGCTACCAG 
GACACCATCC AGCAGCTGGA AAATGAGCTT CGGGGCACAA AGTGGGAAAT GGCTCGTCAT 
CTGCGAGAAT ACCAGGATCT CCTCAACGTC AAGATGGCTC TGGATATTGA GATCGCGGCG 
TACAGGAAAC TCCTGGAGGG TGAAGAGACC AGATTTAGCA CATTTGCAGG TAGCATCACT 
GGGCCACTGT ATACACACCG ACAGCCCTCC ATCGCCATAT CCAGTAAGAT TCAGAAAACC 
AAGGTAGAGG CTCCCAAGCT AAAGGTCCAA CACAAATTTG TTGAGGAGAT TATAGAGGAA 
ACCAAGGTGG AAGATGAGAA ATCAGAAATG GAAGAAGCCC TGACGGCCAT TACCGAGGAA 
TTGGCCGTTT CCGTGAAAGA GGAGGTCAAG GAAGAGGAGG CTGAAGAAAA GGAGGAGAAA 
GAAGAAGCCG AAGAAGAAGT TGTTGCTGCC AAAAAGTCTC CAGTGAAAGC TACTGCACCT 
GAACTTAAAG AAGAGGAAGG AGAAAAGGAG GAGGAAGAGG GCCAAGAGGA AGAGGAAGAG 
GAAGAAGAGG CTGCTAAGTC AGACCAAGCC GAGGAAGGAG GATCTGAGAA GGAAGGTTCT 
AGTGAAAAAG AGGAAGGTGA GCAAGAAGAG GAAGGAGAAA CAGAGGCTGA GGGGGAAGGA 
GAGGAAGCCG CTGCCGAAGC TAAGGAGGAA AAGAAAATGG AGGAAAAGGC TGAAGAAGTG 
GCTCCAAAGG AGGAGCTGGC GGCAGAAGCC AAGGTGGAGA AGCCAGAGAA AGCCAAGTCC 
CCAGTGGCCA AGTCCCCAAC AACAAAGTCC CCAACGGCCA AGTCCCCAGA GGCAAAGTCC 
CCAGAGGCAA AGTCCCCAAC AGCAAAATCC CCGACGGCCA AGTCCCCAGC AGCAAAGTCC 
CCAGCGGCAA AATCACCTGT GGAGGAAGTG AAACCCAAAG CAGAAGCTGG AGCTGAGAAA 
GGAGAACAGA AGGAGAAGGT GGAGGAAGAA AAGAAAGAAG CAAAGGAATC TCCCAAGGAA 
GAGAAGGCAG AGAAAAAGGA GGAGAAGGCA AAGGATGTGC CAGAGAAGAA GAAGGCTGAA 
TCCCCAGTGA AGGCTGAGTC CCCAGTGAAG GAGGAGGTGC CTGCCAAGCC AGTAAAGGTG 
AGCCCAGAGA AGGAAGCCAA AGAGGAGGAG AAGCCACAGG AGAAAGAGAA GGAGAAGGAG 
AAAGTGGAAG AGGTGGGAGG GAAGGAGGAG GGAGGTTTGA AGGAATCCAG GAAGGAAGAC 
ATAGCCATCA ATGGGGAGGT GGAAGGGAAG GAGGAAGAAC AGGAAACTAA GGAGAAAGGC 
AGTGGGGGAG AAGAGGAGAA AGGAGTCGTC ACCAACGGGC TAGACGTGAG CCCAGGGGAT 
GAAAAGAAGG GCGGTGATAA AAGTGAGGAG AAAGTGGTGG TAACCAAAAT GGTGGAAAAA 
ATCACCAGTG AGGGGGGAGA TGGTGCTACC AAGTATATCA CCAAATCTGT AACCGTCACT 
CAAAAGGTCG AAGAGCATGA AGAGACCTTT GAGGAGAAAC TAGTGTCTAC TAAAAAGGTA 
GAGAAAGTCA CTTCACACGC CATAGTAAAG GAAGTCACCC AGAGTGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851209.1
CDS	62..2752
Translation	MSYTLDSLGNPSAYRRVTETRSSFSRISGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLTYSSAMLSSAESSLDFSQSSSLLNGGSGPGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEESSLVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRQPSIAISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSVKEEVKEEEAEEKEEKEEAEEEVVAAKKSPVKATAPELKEEEGEKEEEEGQEEEEEEEEAAKSDQAEEGGSEKEGSSEKEEGEQEEEGETEAEGEGEEAAAEAKEEKKMEEKAEEVAPKEELAAEAKVEKPEKAKSPVAKSPTTKSPTAKSPEAKSPEAKSPTAKSPTAKSPAAKSPAAKSPVEEVKPKAEAGAEKGEQKEKVEEEKKEAKESPKEEKAEKKEEKAKDVPEKKKAESPVKAESPVKEEVPAKPVKVSPEKEAKEEEKPQEKEKEKEKVEEVGGKEEGGLKESRKEDIAINGEVEGKEEEQETKEKGSGGEEEKGVVTNGLDVSPGDEKKGGDKSEEKVVVTKMVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD

Target ORF information:

RefSeq Version	XM_024995441.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neurofilament medium (NEFM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995441.1

ORF Insert Sequence:

ATGAGCTACA CGTTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT GACCGAAACC 
CGCTCGAGCT TCAGCCGCAT CAGCGGCTCC CCGTCCAGCG GCTTCCGCTC GCAGTCGTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT GGCCCCGCGC 
CTCACCTACA GCTCCGCCAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCTCTGC TTAACGGCGG TTCTGGGCCT GGAGGCGACT ACAAGCTGTC CCGCTCCAAC 
GAGAAGGAGC AGCTGCAGGG GCTGAACGAC CGCTTCGCGG GCTACATCGA GAAGGTCCAC 
TACCTGGAGC AGCAGAACAA GGAGATCGAG GCAGAGATCC AGGCGCTGCG GCAGAAGCAG 
GCCTCGCACG CCCAGCTGGG CGACGCGTAC GACCAGGAAA TCCGCGAGCT ACGCGCCACC 
CTGGAGATGG TGAACCATGA GAAGGCTCAG GTACAGCTGG ACTCGGACCA CCTGGAAGAG 
GATATCCACC GGCTCAAGGA GCGCTTCGAG GAGGAGGCAC GGCTGCGCGA CGACACCGAG 
GCGGCTATCC GCGCGCTGCG CAAAGATATC GAGGAGTCGT CGCTGGTCAA GGTGGAGCTG 
GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA TCACGAGGAG 
GAGGTGGCCG ACCTGCTGGC CCAGATCCAA GCGTCACACA TCACCGTGGA GCGCAAAGAC 
TACCTGAAGA CGGACATCTC GACGGCGCTG AAAGAGATCC GCTCCCAGCT CGAGAGTCAC 
TCCGACCAGA ACATGCACCA GGCCGAAGAG TGGTTTAAGT GCCGTTACGC CAAGCTCACC 
GAGGCGGCCG AGCAGAACAA GGAAGCCATC CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC 
CGGCGCCAGC TGCAGTCCAA GAGCATCGAG CTCGAGTCAG TGCGCGGCAC CAAGGAGTCC 
CTGGAGCGGC AGCTCAGCGA CATCGAGGAG CGCCACAACC ACGACCTTAG CAGCTACCAG 
GACACCATCC AGCAGCTGGA AAATGAGCTT CGGGGCACAA AGTGGGAAAT GGCTCGTCAT 
CTGCGAGAAT ACCAGGATCT CCTCAACGTC AAGATGGCTC TGGATATTGA GATCGCGGCG 
TACAGGAAAC TCCTGGAGGG TGAAGAGACC AGATTTAGCA CATTTGCAGG TAGCATCACT 
GGGCCACTGT ATACACACCG ACAGCCCTCC ATCGCCATAT CCAGTAAGAT TCAGAAAACC 
AAGGTAGAGG CTCCCAAGCT AAAGGTCCAA CACAAATTTG TTGAGGAGAT TATAGAGGAA 
ACCAAGGTGG AAGATGAGAA ATCAGAAATG GAAGAAGCCC TGACGGCCAT TACCGAGGAA 
TTGGCCGTTT CCGTGAAAGA GGAGGTCAAG GAAGAGGAGG CTGAAGAAAA GGAGGAGAAA 
GAAGAAGCCG AAGAAGAAGT TGTTGCTGCC AAAAAGTCTC CAGTGAAAGC TACTGCACCT 
GAACTTAAAG AAGAGGAAGG AGAAAAGGAG GAGGAAGAGG GCCAAGAGGA AGAGGAAGAG 
GAAGAAGAGG CTGCTAAGTC AGACCAAGCC GAGGAAGGAG GATCTGAGAA GGAAGGTTCT 
AGTGAAAAAG AGGAAGGTGA GCAAGAAGAG GAAGGAGAAA CAGAGGCTGA GGGGGAAGGA 
GAGGAAGCCG CTGCCGAAGC TAAGGAGGAA AAGAAAATGG AGGAAAAGGC TGAAGAAGTG 
GCTCCAAAGG AGGAGCTGGC GGCAGAAGCC AAGGTGGAGA AGCCAGAGAA AGCCAAGTCC 
CCAGTGGCCA AGTCCCCAAC AACAAAGTCC CCAACGGCCA AGTCCCCAGA GGCAAAGTCC 
CCAGAGGCAA AGTCCCCAAC AGCAAAATCC CCGACGGCCA AGTCCCCAGC AGCAAAGTCC 
CCAGCGGCAA AATCACCTGT GGAGGAAGTG AAACCCAAAG CAGAAGCTGG AGCTGAGAAA 
GGAGAACAGA AGGAGAAGGT GGAGGAAGAA AAGAAAGAAG CAAAGGAATC TCCCAAGGAA 
GAGAAGGCAG AGAAAAAGGA GGAGAAGGCA AAGGATGTGC CAGAGAAGAA GAAGGCTGAA 
TCCCCAGTGA AGGCTGAGTC CCCAGTGAAG GAGGAGGTGC CTGCCAAGCC AGTAAAGGTG 
AGCCCAGAGA AGGAAGCCAA AGAGGAGGAG AAGCCACAGG AGAAAGAGAA GGAGAAGGAG 
AAAGTGGAAG AGGTGGGAGG GAAGGAGGAG GGAGGTTTGA AGGAATCCAG GAAGGAAGAC 
ATAGCCATCA ATGGGGAGGT GGAAGGGAAG GAGGAAGAAC AGGAAACTAA GGAGAAAGGC 
AGTGGGGGAG AAGAGGAGAA AGGAGTCGTC ACCAACGGGC TAGACGTGAG CCCAGGGGAT 
GAAAAGAAGG GCGGTGATAA AAGTGAGGAG AAAGTGGTGG TAACCAAAAT GGTGGAAAAA 
ATCACCAGTG AGGGGGGAGA TGGTGCTACC AAGTATATCA CCAAATCTGT AACCGTCACT 
CAAAAGGTCG AAGAGCATGA AGAGACCTTT GAGGAGAAAC TAGTGTCTAC TAAAAAGGTA 
GAGAAAGTCA CTTCACACGC CATAGTAAAG GAAGTCACCC AGAGTGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of the bovine neurofilament NF-M protein and cDNA sequence, and identification of in vitro and in vivo calpain cleavage sites.
Biochemical and biophysical research communications325(2)619-25(2004 Dec)
Shaw G,Yang C,Zhang L,Cook P,Pike B,Hill WD

Identification of endogenous phosphorylation sites of bovine medium and low molecular weight neurofilament proteins by tandem mass spectrometry.
Biochemistry43(7)2091-105(2004 Feb)
Trimpin S,Mixon AE,Stapels MD,Kim MY,Spencer PS,Deinzer ML

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NEFM cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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