NEFM cDNA ORF clone, Homo sapiens(human)

The following NEFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu12503 NM_001105541.2
Latest version!
Homo sapiens neurofilament medium (NEFM), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $265.30
$379.00
OHu25552 NM_005382.2
Latest version!
Homo sapiens neurofilament medium (NEFM), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
View Old Accession Versions >>
OHu12503 NM_001105541.1 Homo sapiens neurofilament medium (NEFM), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu12503
    Clone ID Related Accession (Same CDS sequence) NM_001105541.1 , NM_001105541.2
    Accession Version NM_001105541.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-12
    Organism Homo sapiens(human)
    Product neurofilament medium polypeptide isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC332005.1, DC352660.1, DC318935.1, AF181990.1, BP247321.1, AF106564.3 and BI496345.1. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus when compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DC332005.1, SRR1803612.142618.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCTCGTC ATTTGCGCGA ATACCAGGAC CTCCTCAACG TCAAGATGGC TCTGGATATA 
    GAAATCGCTG CGTACAGAAA ACTCCTGGAG GGTGAAGAGA CTAGATTTAG CACATTTGCA
    GGAAGCATCA CTGGGCCACT GTATACACAC CGACCCCCAA TCACAATATC CAGTAAGATT
    CAGAAACCCA AGGTGGAAGC TCCCAAGCTT AAGGTCCAAC ACAAATTTGT CGAGGAGATC
    ATAGAGGAAA CCAAAGTGGA GGATGAGAAG TCAGAAATGG AAGAGGCCCT GACAGCCATT
    ACAGAGGAAT TGGCCGTTTC CATGAAGGAA GAGAAGAAAG AAGCAGCAGA AGAAAAGGAA
    GAGGAACCCG AAGCTGAAGA AGAAGAAGTA GCTGCCAAAA AGTCTCCAGT GAAAGCAACT
    GCACCTGAAG TTAAAGAAGA GGAAGGGGAA AAGGAGGAAG AAGAAGGCCA GGAAGAAGAG
    GAGGAAGAAG ATGAGGGAGC TAAGTCAGAC CAAGCCGAAG AGGGAGGATC CGAGAAGGAA
    GGCTCTAGTG AAAAAGAGGA AGGTGAGCAG GAAGAAGGAG AAACAGAAGC TGAAGCTGAA
    GGAGAGGAAG CCGAAGCTAA AGAGGAAAAG AAAGTGGAGG AAAAGAGTGA GGAAGTGGCT
    ACCAAGGAGG AGCTGGTGGC AGATGCCAAG GTGGAAAAGC CAGAAAAAGC CAAGTCTCCT
    GTGCCAAAAT CACCAGTGGA AGAGAAAGGC AAGTCTCCTG TGCCCAAGTC ACCAGTGGAA
    GAGAAAGGCA AGTCTCCTGT GCCCAAGTCA CCAGTGGAAG AGAAAGGCAA GTCTCCTGTG
    CCGAAATCAC CAGTGGAAGA GAAAGGCAAG TCTCCTGTGT CAAAATCACC AGTGGAAGAG
    AAAGCCAAAT CTCCTGTGCC AAAATCACCA GTGGAAGAGG CAAAGTCAAA AGCAGAAGTG
    GGGAAAGGTG AACAGAAAGA GGAAGAAGAA AAGGAAGTCA AGGAAGCTCC CAAGGAAGAG
    AAGGTAGAGA AAAAGGAAGA GAAACCAAAG GATGTGCCAG AGAAGAAGAA AGCTGAGTCC
    CCTGTAAAGG AGGAAGCTGT GGCAGAGGTG GTCACCATCA CCAAATCGGT AAAGGTGCAC
    TTGGAGAAAG AGACCAAAGA AGAGGGGAAG CCACTGCAGC AGGAGAAAGA GAAGGAGAAA
    GCGGGAGGAG AGGGAGGAAG TGAGGAGGAA GGGAGTGATA AAGGTGCCAA GGGATCCAGG
    AAGGAAGACA TAGCTGTCAA TGGGGAGGTA GAAGGAAAAG AGGAGGTAGA GCAGGAGACC
    AAGGAAAAAG GCAGTGGGAG GGAAGAGGAG AAAGGCGTTG TCACCAATGG CCTAGACTTG
    AGCCCAGCAG ATGAAAAGAA GGGGGGTGAT AAAAGTGAGG AGAAAGTGGT GGTGACCAAA
    ACGGTAGAAA AAATCACCAG TGAGGGGGGA GATGGTGCTA CCAAATACAT CACTAAATCT
    GTAACCGTCA CTCAAAAGGT TGAAGAGCAT GAAGAGACCT TTGAGGAGAA ACTAGTGTCT
    ACTAAAAAGG TAGAAAAAGT CACTTCACAC GCCATAGTAA AGGAAGTCAC CCAGAGTGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001099011.1
    CDS405..2027
    Translation

    Target ORF information:

    RefSeq Version NM_001105541.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofilament medium (NEFM), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001105541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCTCGTC ATTTGCGCGA ATACCAGGAC CTCCTCAACG TCAAGATGGC TCTGGATATA 
    GAAATCGCTG CGTACAGAAA ACTCCTGGAG GGTGAAGAGA CTAGATTTAG CACATTTGCA
    GGAAGCATCA CTGGGCCACT GTATACACAC CGACCCCCAA TCACAATATC CAGTAAGATT
    CAGAAACCCA AGGTGGAAGC TCCCAAGCTT AAGGTCCAAC ACAAATTTGT CGAGGAGATC
    ATAGAGGAAA CCAAAGTGGA GGATGAGAAG TCAGAAATGG AAGAGGCCCT GACAGCCATT
    ACAGAGGAAT TGGCCGTTTC CATGAAGGAA GAGAAGAAAG AAGCAGCAGA AGAAAAGGAA
    GAGGAACCCG AAGCTGAAGA AGAAGAAGTA GCTGCCAAAA AGTCTCCAGT GAAAGCAACT
    GCACCTGAAG TTAAAGAAGA GGAAGGGGAA AAGGAGGAAG AAGAAGGCCA GGAAGAAGAG
    GAGGAAGAAG ATGAGGGAGC TAAGTCAGAC CAAGCCGAAG AGGGAGGATC CGAGAAGGAA
    GGCTCTAGTG AAAAAGAGGA AGGTGAGCAG GAAGAAGGAG AAACAGAAGC TGAAGCTGAA
    GGAGAGGAAG CCGAAGCTAA AGAGGAAAAG AAAGTGGAGG AAAAGAGTGA GGAAGTGGCT
    ACCAAGGAGG AGCTGGTGGC AGATGCCAAG GTGGAAAAGC CAGAAAAAGC CAAGTCTCCT
    GTGCCAAAAT CACCAGTGGA AGAGAAAGGC AAGTCTCCTG TGCCCAAGTC ACCAGTGGAA
    GAGAAAGGCA AGTCTCCTGT GCCCAAGTCA CCAGTGGAAG AGAAAGGCAA GTCTCCTGTG
    CCGAAATCAC CAGTGGAAGA GAAAGGCAAG TCTCCTGTGT CAAAATCACC AGTGGAAGAG
    AAAGCCAAAT CTCCTGTGCC AAAATCACCA GTGGAAGAGG CAAAGTCAAA AGCAGAAGTG
    GGGAAAGGTG AACAGAAAGA GGAAGAAGAA AAGGAAGTCA AGGAAGCTCC CAAGGAAGAG
    AAGGTAGAGA AAAAGGAAGA GAAACCAAAG GATGTGCCAG AGAAGAAGAA AGCTGAGTCC
    CCTGTAAAGG AGGAAGCTGT GGCAGAGGTG GTCACCATCA CCAAATCGGT AAAGGTGCAC
    TTGGAGAAAG AGACCAAAGA AGAGGGGAAG CCACTGCAGC AGGAGAAAGA GAAGGAGAAA
    GCGGGAGGAG AGGGAGGAAG TGAGGAGGAA GGGAGTGATA AAGGTGCCAA GGGATCCAGG
    AAGGAAGACA TAGCTGTCAA TGGGGAGGTA GAAGGAAAAG AGGAGGTAGA GCAGGAGACC
    AAGGAAAAAG GCAGTGGGAG GGAAGAGGAG AAAGGCGTTG TCACCAATGG CCTAGACTTG
    AGCCCAGCAG ATGAAAAGAA GGGGGGTGAT AAAAGTGAGG AGAAAGTGGT GGTGACCAAA
    ACGGTAGAAA AAATCACCAG TGAGGGGGGA GATGGTGCTA CCAAATACAT CACTAAATCT
    GTAACCGTCA CTCAAAAGGT TGAAGAGCAT GAAGAGACCT TTGAGGAGAA ACTAGTGTCT
    ACTAAAAAGG TAGAAAAAGT CACTTCACAC GCCATAGTAA AGGAAGTCAC CCAGAGTGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12503
    Clone ID Related Accession (Same CDS sequence) NM_001105541.1 , NM_001105541.2
    Accession Version NM_001105541.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Homo sapiens(human)
    Product neurofilament medium polypeptide isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC332005.1, DC352660.1, DC318935.1, AF181990.1, BP247321.1, AF106564.3 and BI496345.1. On May 31, 2019 this sequence version replaced NM_001105541.1. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus when compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.201414.1, SRR1803614.329732.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCTCGTC ATTTGCGCGA ATACCAGGAC CTCCTCAACG TCAAGATGGC TCTGGATATA 
    GAAATCGCTG CGTACAGAAA ACTCCTGGAG GGTGAAGAGA CTAGATTTAG CACATTTGCA
    GGAAGCATCA CTGGGCCACT GTATACACAC CGACCCCCAA TCACAATATC CAGTAAGATT
    CAGAAACCCA AGGTGGAAGC TCCCAAGCTT AAGGTCCAAC ACAAATTTGT CGAGGAGATC
    ATAGAGGAAA CCAAAGTGGA GGATGAGAAG TCAGAAATGG AAGAGGCCCT GACAGCCATT
    ACAGAGGAAT TGGCCGTTTC CATGAAGGAA GAGAAGAAAG AAGCAGCAGA AGAAAAGGAA
    GAGGAACCCG AAGCTGAAGA AGAAGAAGTA GCTGCCAAAA AGTCTCCAGT GAAAGCAACT
    GCACCTGAAG TTAAAGAAGA GGAAGGGGAA AAGGAGGAAG AAGAAGGCCA GGAAGAAGAG
    GAGGAAGAAG ATGAGGGAGC TAAGTCAGAC CAAGCCGAAG AGGGAGGATC CGAGAAGGAA
    GGCTCTAGTG AAAAAGAGGA AGGTGAGCAG GAAGAAGGAG AAACAGAAGC TGAAGCTGAA
    GGAGAGGAAG CCGAAGCTAA AGAGGAAAAG AAAGTGGAGG AAAAGAGTGA GGAAGTGGCT
    ACCAAGGAGG AGCTGGTGGC AGATGCCAAG GTGGAAAAGC CAGAAAAAGC CAAGTCTCCT
    GTGCCAAAAT CACCAGTGGA AGAGAAAGGC AAGTCTCCTG TGCCCAAGTC ACCAGTGGAA
    GAGAAAGGCA AGTCTCCTGT GCCCAAGTCA CCAGTGGAAG AGAAAGGCAA GTCTCCTGTG
    CCGAAATCAC CAGTGGAAGA GAAAGGCAAG TCTCCTGTGT CAAAATCACC AGTGGAAGAG
    AAAGCCAAAT CTCCTGTGCC AAAATCACCA GTGGAAGAGG CAAAGTCAAA AGCAGAAGTG
    GGGAAAGGTG AACAGAAAGA GGAAGAAGAA AAGGAAGTCA AGGAAGCTCC CAAGGAAGAG
    AAGGTAGAGA AAAAGGAAGA GAAACCAAAG GATGTGCCAG AGAAGAAGAA AGCTGAGTCC
    CCTGTAAAGG AGGAAGCTGT GGCAGAGGTG GTCACCATCA CCAAATCGGT AAAGGTGCAC
    TTGGAGAAAG AGACCAAAGA AGAGGGGAAG CCACTGCAGC AGGAGAAAGA GAAGGAGAAA
    GCGGGAGGAG AGGGAGGAAG TGAGGAGGAA GGGAGTGATA AAGGTGCCAA GGGATCCAGG
    AAGGAAGACA TAGCTGTCAA TGGGGAGGTA GAAGGAAAAG AGGAGGTAGA GCAGGAGACC
    AAGGAAAAAG GCAGTGGGAG GGAAGAGGAG AAAGGCGTTG TCACCAATGG CCTAGACTTG
    AGCCCAGCAG ATGAAAAGAA GGGGGGTGAT AAAAGTGAGG AGAAAGTGGT GGTGACCAAA
    ACGGTAGAAA AAATCACCAG TGAGGGGGGA GATGGTGCTA CCAAATACAT CACTAAATCT
    GTAACCGTCA CTCAAAAGGT TGAAGAGCAT GAAGAGACCT TTGAGGAGAA ACTAGTGTCT
    ACTAAAAAGG TAGAAAAAGT CACTTCACAC GCCATAGTAA AGGAAGTCAC CCAGAGTGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001099011.1
    CDS360..1982
    Translation

    Target ORF information:

    RefSeq Version NM_001105541.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofilament medium (NEFM), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001105541.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCTCGTC ATTTGCGCGA ATACCAGGAC CTCCTCAACG TCAAGATGGC TCTGGATATA 
    GAAATCGCTG CGTACAGAAA ACTCCTGGAG GGTGAAGAGA CTAGATTTAG CACATTTGCA
    GGAAGCATCA CTGGGCCACT GTATACACAC CGACCCCCAA TCACAATATC CAGTAAGATT
    CAGAAACCCA AGGTGGAAGC TCCCAAGCTT AAGGTCCAAC ACAAATTTGT CGAGGAGATC
    ATAGAGGAAA CCAAAGTGGA GGATGAGAAG TCAGAAATGG AAGAGGCCCT GACAGCCATT
    ACAGAGGAAT TGGCCGTTTC CATGAAGGAA GAGAAGAAAG AAGCAGCAGA AGAAAAGGAA
    GAGGAACCCG AAGCTGAAGA AGAAGAAGTA GCTGCCAAAA AGTCTCCAGT GAAAGCAACT
    GCACCTGAAG TTAAAGAAGA GGAAGGGGAA AAGGAGGAAG AAGAAGGCCA GGAAGAAGAG
    GAGGAAGAAG ATGAGGGAGC TAAGTCAGAC CAAGCCGAAG AGGGAGGATC CGAGAAGGAA
    GGCTCTAGTG AAAAAGAGGA AGGTGAGCAG GAAGAAGGAG AAACAGAAGC TGAAGCTGAA
    GGAGAGGAAG CCGAAGCTAA AGAGGAAAAG AAAGTGGAGG AAAAGAGTGA GGAAGTGGCT
    ACCAAGGAGG AGCTGGTGGC AGATGCCAAG GTGGAAAAGC CAGAAAAAGC CAAGTCTCCT
    GTGCCAAAAT CACCAGTGGA AGAGAAAGGC AAGTCTCCTG TGCCCAAGTC ACCAGTGGAA
    GAGAAAGGCA AGTCTCCTGT GCCCAAGTCA CCAGTGGAAG AGAAAGGCAA GTCTCCTGTG
    CCGAAATCAC CAGTGGAAGA GAAAGGCAAG TCTCCTGTGT CAAAATCACC AGTGGAAGAG
    AAAGCCAAAT CTCCTGTGCC AAAATCACCA GTGGAAGAGG CAAAGTCAAA AGCAGAAGTG
    GGGAAAGGTG AACAGAAAGA GGAAGAAGAA AAGGAAGTCA AGGAAGCTCC CAAGGAAGAG
    AAGGTAGAGA AAAAGGAAGA GAAACCAAAG GATGTGCCAG AGAAGAAGAA AGCTGAGTCC
    CCTGTAAAGG AGGAAGCTGT GGCAGAGGTG GTCACCATCA CCAAATCGGT AAAGGTGCAC
    TTGGAGAAAG AGACCAAAGA AGAGGGGAAG CCACTGCAGC AGGAGAAAGA GAAGGAGAAA
    GCGGGAGGAG AGGGAGGAAG TGAGGAGGAA GGGAGTGATA AAGGTGCCAA GGGATCCAGG
    AAGGAAGACA TAGCTGTCAA TGGGGAGGTA GAAGGAAAAG AGGAGGTAGA GCAGGAGACC
    AAGGAAAAAG GCAGTGGGAG GGAAGAGGAG AAAGGCGTTG TCACCAATGG CCTAGACTTG
    AGCCCAGCAG ATGAAAAGAA GGGGGGTGAT AAAAGTGAGG AGAAAGTGGT GGTGACCAAA
    ACGGTAGAAA AAATCACCAG TGAGGGGGGA GATGGTGCTA CCAAATACAT CACTAAATCT
    GTAACCGTCA CTCAAAAGGT TGAAGAGCAT GAAGAGACCT TTGAGGAGAA ACTAGTGTCT
    ACTAAAAAGG TAGAAAAAGT CACTTCACAC GCCATAGTAA AGGAAGTCAC CCAGAGTGAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25552
    Clone ID Related Accession (Same CDS sequence) NM_005382.2
    Accession Version NM_005382.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Homo sapiens(human)
    Product neurofilament medium polypeptide isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC071752.1, BC096757.1 and BP311096.1. This sequence is a reference standard in the RefSeqGene project. On Sep 28, 2007 this sequence version replaced NM_005382.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.88845.1, SRR1803616.189930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000221166.10/ ENSP00000221166.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGCTACA CGTTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT AACCGAGACC 
    CGCTCGAGCT TCAGCCGCGT CAGCGGCTCC CCGTCCAGTG GCTTCCGCTC GCAGTCGTGG
    TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTATAAGC GCAGCATGCT CGCCCCGCGC
    CTCGCTTACA GCTCGGCCAT GCTCAGCTCC GCCGAGAGCA GCCTTGACTT CAGCCAGTCC
    TCGTCCCTGC TCAACGGCGG CTCCGGACCC GGCGGCGACT ACAAGCTGTC CCGCTCCAAC
    GAGAAGGAGC AGCTGCAGGG GCTGAACGAC CGCTTTGCCG GCTACATAGA GAAGGTGCAC
    TACCTGGAGC AGCAGAATAA GGAGATTGAG GCGGAGATCC AGGCGCTGCG GCAGAAGCAG
    GCCTCGCACG CCCAGCTGGG CGACGCGTAC GACCAGGAGA TCCGCGAGCT GCGCGCCACC
    CTGGAGATGG TGAACCACGA GAAGGCTCAG GTGCAGCTGG ACTCGGACCA CCTGGAGGAA
    GACATCCACC GGCTCAAGGA GCGCTTTGAG GAGGAGGCGC GGTTGCGCGA CGACACTGAG
    GCGGCCATCC GCGCGCTGCG CAAAGACATC GAGGAGGCGT CGCTGGTCAA GGTGGAGCTG
    GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA CCACGAGGAG
    GAGGTGGCCG ACCTTCTGGC CCAGATCCAG GCATCGCACA TCACGGTGGA GCGCAAAGAC
    TACCTGAAGA CAGACATCTC GACGGCGCTG AAGGAAATCC GCTCCCAGCT CGAAAGCCAC
    TCAGACCAGA ATATGCACCA GGCCGAAGAG TGGTTCAAAT GCCGCTACGC CAAGCTCACC
    GAGGCGGCCG AGCAGAACAA GGAGGCCATC CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC
    CGGCGCCAGC TGCAGTCCAA GAGCATCGAG CTAGAGTCGG TGCGCGGCAC CAAGGAGTCC
    CTGGAGCGGC AGCTCAGCGA CATCGAGGAG CGCCACAACC ACGACCTCAG CAGCTACCAG
    GACACCATCC AGCAGCTGGA AAATGAGCTT CGGGGCACAA AGTGGGAAAT GGCTCGTCAT
    TTGCGCGAAT ACCAGGACCT CCTCAACGTC AAGATGGCTC TGGATATAGA AATCGCTGCG
    TACAGAAAAC TCCTGGAGGG TGAAGAGACT AGATTTAGCA CATTTGCAGG AAGCATCACT
    GGGCCACTGT ATACACACCG ACCCCCAATC ACAATATCCA GTAAGATTCA GAAACCCAAG
    GTGGAAGCTC CCAAGCTTAA GGTCCAACAC AAATTTGTCG AGGAGATCAT AGAGGAAACC
    AAAGTGGAGG ATGAGAAGTC AGAAATGGAA GAGGCCCTGA CAGCCATTAC AGAGGAATTG
    GCCGTTTCCA TGAAGGAAGA GAAGAAAGAA GCAGCAGAAG AAAAGGAAGA GGAACCCGAA
    GCTGAAGAAG AAGAAGTAGC TGCCAAAAAG TCTCCAGTGA AAGCAACTGC ACCTGAAGTT
    AAAGAAGAGG AAGGGGAAAA GGAGGAAGAA GAAGGCCAGG AAGAAGAGGA GGAAGAAGAT
    GAGGGAGCTA AGTCAGACCA AGCCGAAGAG GGAGGATCCG AGAAGGAAGG CTCTAGTGAA
    AAAGAGGAAG GTGAGCAGGA AGAAGGAGAA ACAGAAGCTG AAGCTGAAGG AGAGGAAGCC
    GAAGCTAAAG AGGAAAAGAA AGTGGAGGAA AAGAGTGAGG AAGTGGCTAC CAAGGAGGAG
    CTGGTGGCAG ATGCCAAGGT GGAAAAGCCA GAAAAAGCCA AGTCTCCTGT GCCAAAATCA
    CCAGTGGAAG AGAAAGGCAA GTCTCCTGTG CCCAAGTCAC CAGTGGAAGA GAAAGGCAAG
    TCTCCTGTGC CCAAGTCACC AGTGGAAGAG AAAGGCAAGT CTCCTGTGCC GAAATCACCA
    GTGGAAGAGA AAGGCAAGTC TCCTGTGTCA AAATCACCAG TGGAAGAGAA AGCCAAATCT
    CCTGTGCCAA AATCACCAGT GGAAGAGGCA AAGTCAAAAG CAGAAGTGGG GAAAGGTGAA
    CAGAAAGAGG AAGAAGAAAA GGAAGTCAAG GAAGCTCCCA AGGAAGAGAA GGTAGAGAAA
    AAGGAAGAGA AACCAAAGGA TGTGCCAGAG AAGAAGAAAG CTGAGTCCCC TGTAAAGGAG
    GAAGCTGTGG CAGAGGTGGT CACCATCACC AAATCGGTAA AGGTGCACTT GGAGAAAGAG
    ACCAAAGAAG AGGGGAAGCC ACTGCAGCAG GAGAAAGAGA AGGAGAAAGC GGGAGGAGAG
    GGAGGAAGTG AGGAGGAAGG GAGTGATAAA GGTGCCAAGG GATCCAGGAA GGAAGACATA
    GCTGTCAATG GGGAGGTAGA AGGAAAAGAG GAGGTAGAGC AGGAGACCAA GGAAAAAGGC
    AGTGGGAGGG AAGAGGAGAA AGGCGTTGTC ACCAATGGCC TAGACTTGAG CCCAGCAGAT
    GAAAAGAAGG GGGGTGATAA AAGTGAGGAG AAAGTGGTGG TGACCAAAAC GGTAGAAAAA
    ATCACCAGTG AGGGGGGAGA TGGTGCTACC AAATACATCA CTAAATCTGT AACCGTCACT
    CAAAAGGTTG AAGAGCATGA AGAGACCTTT GAGGAGAAAC TAGTGTCTAC TAAAAAGGTA
    GAAAAAGTCA CTTCACACGC CATAGTAAAG GAAGTCACCC AGAGTGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005373.2
    CDS34..2784
    Translation

    Target ORF information:

    RefSeq Version NM_005382.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofilament medium (NEFM), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005382.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGCTACA CGTTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT AACCGAGACC 
    CGCTCGAGCT TCAGCCGCGT CAGCGGCTCC CCGTCCAGTG GCTTCCGCTC GCAGTCGTGG
    TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTATAAGC GCAGCATGCT CGCCCCGCGC
    CTCGCTTACA GCTCGGCCAT GCTCAGCTCC GCCGAGAGCA GCCTTGACTT CAGCCAGTCC
    TCGTCCCTGC TCAACGGCGG CTCCGGACCC GGCGGCGACT ACAAGCTGTC CCGCTCCAAC
    GAGAAGGAGC AGCTGCAGGG GCTGAACGAC CGCTTTGCCG GCTACATAGA GAAGGTGCAC
    TACCTGGAGC AGCAGAATAA GGAGATTGAG GCGGAGATCC AGGCGCTGCG GCAGAAGCAG
    GCCTCGCACG CCCAGCTGGG CGACGCGTAC GACCAGGAGA TCCGCGAGCT GCGCGCCACC
    CTGGAGATGG TGAACCACGA GAAGGCTCAG GTGCAGCTGG ACTCGGACCA CCTGGAGGAA
    GACATCCACC GGCTCAAGGA GCGCTTTGAG GAGGAGGCGC GGTTGCGCGA CGACACTGAG
    GCGGCCATCC GCGCGCTGCG CAAAGACATC GAGGAGGCGT CGCTGGTCAA GGTGGAGCTG
    GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA CCACGAGGAG
    GAGGTGGCCG ACCTTCTGGC CCAGATCCAG GCATCGCACA TCACGGTGGA GCGCAAAGAC
    TACCTGAAGA CAGACATCTC GACGGCGCTG AAGGAAATCC GCTCCCAGCT CGAAAGCCAC
    TCAGACCAGA ATATGCACCA GGCCGAAGAG TGGTTCAAAT GCCGCTACGC CAAGCTCACC
    GAGGCGGCCG AGCAGAACAA GGAGGCCATC CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC
    CGGCGCCAGC TGCAGTCCAA GAGCATCGAG CTAGAGTCGG TGCGCGGCAC CAAGGAGTCC
    CTGGAGCGGC AGCTCAGCGA CATCGAGGAG CGCCACAACC ACGACCTCAG CAGCTACCAG
    GACACCATCC AGCAGCTGGA AAATGAGCTT CGGGGCACAA AGTGGGAAAT GGCTCGTCAT
    TTGCGCGAAT ACCAGGACCT CCTCAACGTC AAGATGGCTC TGGATATAGA AATCGCTGCG
    TACAGAAAAC TCCTGGAGGG TGAAGAGACT AGATTTAGCA CATTTGCAGG AAGCATCACT
    GGGCCACTGT ATACACACCG ACCCCCAATC ACAATATCCA GTAAGATTCA GAAACCCAAG
    GTGGAAGCTC CCAAGCTTAA GGTCCAACAC AAATTTGTCG AGGAGATCAT AGAGGAAACC
    AAAGTGGAGG ATGAGAAGTC AGAAATGGAA GAGGCCCTGA CAGCCATTAC AGAGGAATTG
    GCCGTTTCCA TGAAGGAAGA GAAGAAAGAA GCAGCAGAAG AAAAGGAAGA GGAACCCGAA
    GCTGAAGAAG AAGAAGTAGC TGCCAAAAAG TCTCCAGTGA AAGCAACTGC ACCTGAAGTT
    AAAGAAGAGG AAGGGGAAAA GGAGGAAGAA GAAGGCCAGG AAGAAGAGGA GGAAGAAGAT
    GAGGGAGCTA AGTCAGACCA AGCCGAAGAG GGAGGATCCG AGAAGGAAGG CTCTAGTGAA
    AAAGAGGAAG GTGAGCAGGA AGAAGGAGAA ACAGAAGCTG AAGCTGAAGG AGAGGAAGCC
    GAAGCTAAAG AGGAAAAGAA AGTGGAGGAA AAGAGTGAGG AAGTGGCTAC CAAGGAGGAG
    CTGGTGGCAG ATGCCAAGGT GGAAAAGCCA GAAAAAGCCA AGTCTCCTGT GCCAAAATCA
    CCAGTGGAAG AGAAAGGCAA GTCTCCTGTG CCCAAGTCAC CAGTGGAAGA GAAAGGCAAG
    TCTCCTGTGC CCAAGTCACC AGTGGAAGAG AAAGGCAAGT CTCCTGTGCC GAAATCACCA
    GTGGAAGAGA AAGGCAAGTC TCCTGTGTCA AAATCACCAG TGGAAGAGAA AGCCAAATCT
    CCTGTGCCAA AATCACCAGT GGAAGAGGCA AAGTCAAAAG CAGAAGTGGG GAAAGGTGAA
    CAGAAAGAGG AAGAAGAAAA GGAAGTCAAG GAAGCTCCCA AGGAAGAGAA GGTAGAGAAA
    AAGGAAGAGA AACCAAAGGA TGTGCCAGAG AAGAAGAAAG CTGAGTCCCC TGTAAAGGAG
    GAAGCTGTGG CAGAGGTGGT CACCATCACC AAATCGGTAA AGGTGCACTT GGAGAAAGAG
    ACCAAAGAAG AGGGGAAGCC ACTGCAGCAG GAGAAAGAGA AGGAGAAAGC GGGAGGAGAG
    GGAGGAAGTG AGGAGGAAGG GAGTGATAAA GGTGCCAAGG GATCCAGGAA GGAAGACATA
    GCTGTCAATG GGGAGGTAGA AGGAAAAGAG GAGGTAGAGC AGGAGACCAA GGAAAAAGGC
    AGTGGGAGGG AAGAGGAGAA AGGCGTTGTC ACCAATGGCC TAGACTTGAG CCCAGCAGAT
    GAAAAGAAGG GGGGTGATAA AAGTGAGGAG AAAGTGGTGG TGACCAAAAC GGTAGAAAAA
    ATCACCAGTG AGGGGGGAGA TGGTGCTACC AAATACATCA CTAAATCTGT AACCGTCACT
    CAAAAGGTTG AAGAGCATGA AGAGACCTTT GAGGAGAAAC TAGTGTCTAC TAAAAAGGTA
    GAAAAAGTCA CTTCACACGC CATAGTAAAG GAAGTCACCC AGAGTGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.