S1PR1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	S1PR1
Entrez Gene ID	281135
Full Name	sphingosine-1-phosphate receptor 1
Synonyms	EDG1,S1P1
General protein information	Preferred Names sphingosine-1-phosphate receptor 1 Names sphingosine 1-phosphate receptor 1 S1P receptor 1 S1P receptor Edg-1 endothelial differentiation G-protein coupled receptor 1 endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 sphingosine 1-phosphate receptor Edg-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024984569.1	XP_024840337.1	sphingosine 1-phosphate receptor 1 isoform X1
NM_001013585.4	NP_001013603.2	sphingosine 1-phosphate receptor 1
XM_024984567.1	XP_024840335.1	sphingosine 1-phosphate receptor 1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	S1PR1	NP_001013603.2
Xenopus tropicalis (tropical clawed frog)	s1pr1	NP_001072893.1
Pan troglodytes (chimpanzee)	S1PR1	XP_001138743.2
Mus musculus (house mouse)	S1pr1	NP_031927.2
Gallus gallus (chicken)	S1PR1	XP_004936607.1
Homo sapiens (human)	S1PR1	NP_001391.2
Canis lupus familiaris (dog)	S1PR1	XP_005621915.1
Rattus norvegicus (Norway rat)	S1pr1	NP_058997.1
	S1PR1	XP_001107778.2
Danio rerio (zebrafish)	s1pr1	NP_571766.2

Gene Ontology
Bibliography

Related articles in PubMed

Multiple marker effects of single nucleotide polymorphisms in three genes, AKIRIN2, EDG1 and RPL27A, for marbling development in Japanese Black cattle.
Sukegawa S, Miyake T, Ibi T, Takahagi Y, Murakami H, Morimatsu F, Yamada T
Animal science journal = Nihon chikusan Gakkaiho85(3)193-7(2014 Mar)

Novel SNP in 5' flanking region of EDG1 associated with marbling in Japanese Black beef cattle.
Yamada T, Sasaki S, Sukegawa S, Miyake T, Fujita T, Kose H, Morita M, Takahagi Y, Murakami H, Morimatsu F, Sasaki Y
Animal science journal = Nihon chikusan Gakkaiho80(4)486-9(2009 Aug)

Association of single nucleotide polymorphisms in the endothelial differentiation sphingolipid G-protein-coupled receptor 1 gene with marbling in Japanese Black beef cattle.
Yamada T, Itoh M, Nishimura S, Taniguchi Y, Miyake T, Sasaki S, Yoshioka S, Fujita T, Shiga K, Morita M, Sasaki Y
Animal genetics40(2)209-16(2009 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Polymorphisms in promoter regions of PDHB, SORBS1, and EDG1 genes showing marbling-associated expression changes.
Yamada T, Sasaki S, Sukegawa S, Muramatsu Y, Takahagi Y, Yoshioka S, Fujita T, Morita M, Murakami H, Morimatsu F, Sasaki Y
Research communications in molecular pathology and pharmacology120-121(1-6)105-14(2007-2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The findings suggest the presence of inter-allelic interactions among genes affecting the development of beef marbling.
Title: Multiple marker effects of single nucleotide polymorphisms in three genes, AKIRIN2, EDG1 and RPL27A, for marbling development in Japanese Black cattle.

we detected 2 novel SNPs, referred to as g.1475435G>A and g.1471620G>T, in the 5' flanking region of the EDG1 between low-marbled and high-marbled steer groups
Title: Novel SNP in 5' flanking region of EDG1 associated with marbling in Japanese Black beef cattle.

EDG1 SNPs, although they may not be regarded as a causal mutation, may be useful for effective marker-assisted selection to increase the levels of marbling in Japanese Black beef cattle.
Title: Association of single nucleotide polymorphisms in the endothelial differentiation sphingolipid G-protein-coupled receptor 1 gene with marbling in Japanese Black beef cattle.

Polymorphisms in promoter regions of PDHB, SORBS1, and EDG1 genes showing marbling-associated expression changes.
Title: Polymorphisms in promoter regions of PDHB, SORBS1, and EDG1 genes showing marbling-associated expression changes.

induction by vascular endothelial growth factors in endothelial cells
Title: VEGF induces S1P1 receptors in endothelial cells: Implications for cross-talk between sphingolipid and growth factor receptors.

The following S1PR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the S1PR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44699	NM_001013585.4 Latest version!	Bos taurus sphingosine-1-phosphate receptor 1 (S1PR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa44699	XM_024984569.1 Latest version!	Bos taurus sphingosine-1-phosphate receptor 1 (S1PR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa44699	XM_024984567.1 Latest version!	Bos taurus sphingosine-1-phosphate receptor 1 (S1PR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

S1PR1 ( NM_001013585.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001013603.2 Bos taurus sphingosine-1-phosphate receptor 1 (S1PR1), mRNA.

Price & Availability↑

CloneID	OBa44699
Clone ID Related Accession (Same CDS sequence)	XM_024984569.1 , NM_001013585.4 , XM_024984567.1
Accession Version	NM_001013585.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Bos taurus(cattle)
Product	sphingosine 1-phosphate receptor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC114045.1. On Aug 30, 2012 this sequence version replaced NM_001013585.3. ##Evidence-Data-START## Transcript exon combination :: BC114045.1, BT020877.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGGGTCCA CCCGTATCCC GCTGGTCAAG GCCCTCCACA GCCCTGTCTC GGACTATGTC 
AACTACGACA TCATCGTCCG GCATTATAAC TACACGGGAA AGCTGAAGAT CAGCGCGGAC 
AAGGACAATG GCATTAAACT GATCTCAGTG GTGTTCATTC TCATCTGCTG CTTTATCATC 
CTAGAGAACA TTTTTGTTTT GCTGACCATC TGGAAAACCA AGAAGTTCCA CCGACCCATG 
TACTATTTTA TCGGCAATCT GGCCCTCTCA GACCTGTTGG CGGGAGTGGC CTACACAGCC 
AACCTGCTCT TGTCTGGGGC CACCACCTAC AAGCTAACCC CCGCCCAGTG GTTTCTGCGG 
GAAGGGAGTA TGTTTGTGGC CCTCTCGGCC TCCGTGTTCA GCCTCCTGGC CATCGCCATT 
GAGCGCTACA TCACCATGCT GAAGATGAAA CTCCACAATG GGAGCAACAG GTTCCGCTCC 
TTCCTGCTCA TCAGTGCCTG CTGGGTCATC TCCCTCATCC TGGGGGGCCT GCCTATCATG 
GGCTGGAACT GCATCAGCAC GCTGCCCAGC TGTTCTACTG TGCTGCCGCT CTACCACAAG 
CACTATATCC TCTTCTGCAC CACGGTCTTC ACTCTGCTCC TGCTCTCCAT TGTCATCCTG 
TACTGCAGGA TCTACTCCTT GGTCAGGACT CGCAGCCGCC GTCTGACCTT CCGCAAGAAC 
ATTTCCAAGG CCAGCCGCAG CTCTGAGAAG TCTCTGGCAC TGCTCAAGAC CGTGATTATT 
GTCCTGGGCG TCTTCATCGC CTGCTGGGCA CCACTCTTCA TCCTGCTCCT GCTCGACGTG 
GGCTGCAAGG TGAAGACCTG TGACATCCTC TTCAGAACGG AGTACTTCCT GGTGTTGGCT 
GTGCTCAACT CTGGCACCAA CCCCATCATT TACACTTTGT CCAACAAGGA GATGCGTCGG 
GCCTTCGTCC GGATCATGTC CTGCTGCAAG TGCCCCAGTA GAGACTCCGC GAGTAAATTC 
ACACGACCCA TCATCGCGGG CATGGAATTC AGCCGCAGTA AGTCAGACAA CTCCTCCCAC 
CCCCAGAAGG ACGATGGGGA CAACCCAGAG ACCATTATGT CTTCTGGAAA CGTCAACTCT 
TCTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013603.2
CDS	478..1626
Translation	MGSTRIPLVKALHSPVSDYVNYDIIVRHYNYTGKLKISADKDNGIKLISVVFILICCFIILENIFVLLTIWKTKKFHRPMYYFIGNLALSDLLAGVAYTANLLLSGATTYKLTPAQWFLREGSMFVALSASVFSLLAIAIERYITMLKMKLHNGSNRFRSFLLISACWVISLILGGLPIMGWNCISTLPSCSTVLPLYHKHYILFCTTVFTLLLLSIVILYCRIYSLVRTRSRRLTFRKNISKASRSSEKSLALLKTVIIVLGVFIACWAPLFILLLLDVGCKVKTCDILFRTEYFLVLAVLNSGTNPIIYTLSNKEMRRAFVRIMSCCKCPSRDSASKFTRPIIAGMEFSRSKSDNSSHPQKDDGDNPETIMSSGNVNSSS

Target ORF information:

RefSeq Version	NM_001013585.4
Organism	Bos taurus(cattle)
Definition	Bos taurus sphingosine-1-phosphate receptor 1 (S1PR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013585.4

ORF Insert Sequence:

ATGGGGTCCA CCCGTATCCC GCTGGTCAAG GCCCTCCACA GCCCTGTCTC GGACTATGTC 
AACTACGACA TCATCGTCCG GCATTATAAC TACACGGGAA AGCTGAAGAT CAGCGCGGAC 
AAGGACAATG GCATTAAACT GATCTCAGTG GTGTTCATTC TCATCTGCTG CTTTATCATC 
CTAGAGAACA TTTTTGTTTT GCTGACCATC TGGAAAACCA AGAAGTTCCA CCGACCCATG 
TACTATTTTA TCGGCAATCT GGCCCTCTCA GACCTGTTGG CGGGAGTGGC CTACACAGCC 
AACCTGCTCT TGTCTGGGGC CACCACCTAC AAGCTAACCC CCGCCCAGTG GTTTCTGCGG 
GAAGGGAGTA TGTTTGTGGC CCTCTCGGCC TCCGTGTTCA GCCTCCTGGC CATCGCCATT 
GAGCGCTACA TCACCATGCT GAAGATGAAA CTCCACAATG GGAGCAACAG GTTCCGCTCC 
TTCCTGCTCA TCAGTGCCTG CTGGGTCATC TCCCTCATCC TGGGGGGCCT GCCTATCATG 
GGCTGGAACT GCATCAGCAC GCTGCCCAGC TGTTCTACTG TGCTGCCGCT CTACCACAAG 
CACTATATCC TCTTCTGCAC CACGGTCTTC ACTCTGCTCC TGCTCTCCAT TGTCATCCTG 
TACTGCAGGA TCTACTCCTT GGTCAGGACT CGCAGCCGCC GTCTGACCTT CCGCAAGAAC 
ATTTCCAAGG CCAGCCGCAG CTCTGAGAAG TCTCTGGCAC TGCTCAAGAC CGTGATTATT 
GTCCTGGGCG TCTTCATCGC CTGCTGGGCA CCACTCTTCA TCCTGCTCCT GCTCGACGTG 
GGCTGCAAGG TGAAGACCTG TGACATCCTC TTCAGAACGG AGTACTTCCT GGTGTTGGCT 
GTGCTCAACT CTGGCACCAA CCCCATCATT TACACTTTGT CCAACAAGGA GATGCGTCGG 
GCCTTCGTCC GGATCATGTC CTGCTGCAAG TGCCCCAGTA GAGACTCCGC GAGTAAATTC 
ACACGACCCA TCATCGCGGG CATGGAATTC AGCCGCAGTA AGTCAGACAA CTCCTCCCAC 
CCCCAGAAGG ACGATGGGGA CAACCCAGAG ACCATTATGT CTTCTGGAAA CGTCAACTCT 
TCTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S1PR1 ( XM_024984569.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840337.1 Bos taurus sphingosine-1-phosphate receptor 1 (S1PR1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa44699
Clone ID Related Accession (Same CDS sequence)	XM_024984569.1 , NM_001013585.4 , XM_024984567.1
Accession Version	XM_024984569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sphingosine 1-phosphate receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCCA CCCGTATCCC GCTGGTCAAG GCCCTCCACA GCCCTGTCTC GGACTATGTC 
AACTACGACA TCATCGTCCG GCATTATAAC TACACGGGAA AGCTGAAGAT CAGCGCGGAC 
AAGGACAATG GCATTAAACT GATCTCAGTG GTGTTCATTC TCATCTGCTG CTTTATCATC 
CTAGAGAACA TTTTTGTTTT GCTGACCATC TGGAAAACCA AGAAGTTCCA CCGACCCATG 
TACTATTTTA TCGGCAATCT GGCCCTCTCA GACCTGTTGG CGGGAGTGGC CTACACAGCC 
AACCTGCTCT TGTCTGGGGC CACCACCTAC AAGCTAACCC CCGCCCAGTG GTTTCTGCGG 
GAAGGGAGTA TGTTTGTGGC CCTCTCGGCC TCCGTGTTCA GCCTCCTGGC CATCGCCATT 
GAGCGCTACA TCACCATGCT GAAGATGAAA CTCCACAATG GGAGCAACAG GTTCCGCTCC 
TTCCTGCTCA TCAGTGCCTG CTGGGTCATC TCCCTCATCC TGGGGGGCCT GCCTATCATG 
GGCTGGAACT GCATCAGCAC GCTGCCCAGC TGTTCTACTG TGCTGCCGCT CTACCACAAG 
CACTATATCC TCTTCTGCAC CACGGTCTTC ACTCTGCTCC TGCTCTCCAT TGTCATCCTG 
TACTGCAGGA TCTACTCCTT GGTCAGGACT CGCAGCCGCC GTCTGACCTT CCGCAAGAAC 
ATTTCCAAGG CCAGCCGCAG CTCTGAGAAG TCTCTGGCAC TGCTCAAGAC CGTGATTATT 
GTCCTGGGCG TCTTCATCGC CTGCTGGGCA CCACTCTTCA TCCTGCTCCT GCTCGACGTG 
GGCTGCAAGG TGAAGACCTG TGACATCCTC TTCAGAACGG AGTACTTCCT GGTGTTGGCT 
GTGCTCAACT CTGGCACCAA CCCCATCATT TACACTTTGT CCAACAAGGA GATGCGTCGG 
GCCTTCGTCC GGATCATGTC CTGCTGCAAG TGCCCCAGTA GAGACTCCGC GAGTAAATTC 
ACACGACCCA TCATCGCGGG CATGGAATTC AGCCGCAGTA AGTCAGACAA CTCCTCCCAC 
CCCCAGAAGG ACGATGGGGA CAACCCAGAG ACCATTATGT CTTCTGGAAA CGTCAACTCT 
TCTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840337.1
CDS	382..1530
Translation	MGSTRIPLVKALHSPVSDYVNYDIIVRHYNYTGKLKISADKDNGIKLISVVFILICCFIILENIFVLLTIWKTKKFHRPMYYFIGNLALSDLLAGVAYTANLLLSGATTYKLTPAQWFLREGSMFVALSASVFSLLAIAIERYITMLKMKLHNGSNRFRSFLLISACWVISLILGGLPIMGWNCISTLPSCSTVLPLYHKHYILFCTTVFTLLLLSIVILYCRIYSLVRTRSRRLTFRKNISKASRSSEKSLALLKTVIIVLGVFIACWAPLFILLLLDVGCKVKTCDILFRTEYFLVLAVLNSGTNPIIYTLSNKEMRRAFVRIMSCCKCPSRDSASKFTRPIIAGMEFSRSKSDNSSHPQKDDGDNPETIMSSGNVNSSS

Target ORF information:

RefSeq Version	XM_024984569.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sphingosine-1-phosphate receptor 1 (S1PR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984569.1

ORF Insert Sequence:

ATGGGGTCCA CCCGTATCCC GCTGGTCAAG GCCCTCCACA GCCCTGTCTC GGACTATGTC 
AACTACGACA TCATCGTCCG GCATTATAAC TACACGGGAA AGCTGAAGAT CAGCGCGGAC 
AAGGACAATG GCATTAAACT GATCTCAGTG GTGTTCATTC TCATCTGCTG CTTTATCATC 
CTAGAGAACA TTTTTGTTTT GCTGACCATC TGGAAAACCA AGAAGTTCCA CCGACCCATG 
TACTATTTTA TCGGCAATCT GGCCCTCTCA GACCTGTTGG CGGGAGTGGC CTACACAGCC 
AACCTGCTCT TGTCTGGGGC CACCACCTAC AAGCTAACCC CCGCCCAGTG GTTTCTGCGG 
GAAGGGAGTA TGTTTGTGGC CCTCTCGGCC TCCGTGTTCA GCCTCCTGGC CATCGCCATT 
GAGCGCTACA TCACCATGCT GAAGATGAAA CTCCACAATG GGAGCAACAG GTTCCGCTCC 
TTCCTGCTCA TCAGTGCCTG CTGGGTCATC TCCCTCATCC TGGGGGGCCT GCCTATCATG 
GGCTGGAACT GCATCAGCAC GCTGCCCAGC TGTTCTACTG TGCTGCCGCT CTACCACAAG 
CACTATATCC TCTTCTGCAC CACGGTCTTC ACTCTGCTCC TGCTCTCCAT TGTCATCCTG 
TACTGCAGGA TCTACTCCTT GGTCAGGACT CGCAGCCGCC GTCTGACCTT CCGCAAGAAC 
ATTTCCAAGG CCAGCCGCAG CTCTGAGAAG TCTCTGGCAC TGCTCAAGAC CGTGATTATT 
GTCCTGGGCG TCTTCATCGC CTGCTGGGCA CCACTCTTCA TCCTGCTCCT GCTCGACGTG 
GGCTGCAAGG TGAAGACCTG TGACATCCTC TTCAGAACGG AGTACTTCCT GGTGTTGGCT 
GTGCTCAACT CTGGCACCAA CCCCATCATT TACACTTTGT CCAACAAGGA GATGCGTCGG 
GCCTTCGTCC GGATCATGTC CTGCTGCAAG TGCCCCAGTA GAGACTCCGC GAGTAAATTC 
ACACGACCCA TCATCGCGGG CATGGAATTC AGCCGCAGTA AGTCAGACAA CTCCTCCCAC 
CCCCAGAAGG ACGATGGGGA CAACCCAGAG ACCATTATGT CTTCTGGAAA CGTCAACTCT 
TCTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S1PR1 ( XM_024984567.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840335.1 Bos taurus sphingosine-1-phosphate receptor 1 (S1PR1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa44699
Clone ID Related Accession (Same CDS sequence)	XM_024984569.1 , NM_001013585.4 , XM_024984567.1
Accession Version	XM_024984567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sphingosine 1-phosphate receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCCA CCCGTATCCC GCTGGTCAAG GCCCTCCACA GCCCTGTCTC GGACTATGTC 
AACTACGACA TCATCGTCCG GCATTATAAC TACACGGGAA AGCTGAAGAT CAGCGCGGAC 
AAGGACAATG GCATTAAACT GATCTCAGTG GTGTTCATTC TCATCTGCTG CTTTATCATC 
CTAGAGAACA TTTTTGTTTT GCTGACCATC TGGAAAACCA AGAAGTTCCA CCGACCCATG 
TACTATTTTA TCGGCAATCT GGCCCTCTCA GACCTGTTGG CGGGAGTGGC CTACACAGCC 
AACCTGCTCT TGTCTGGGGC CACCACCTAC AAGCTAACCC CCGCCCAGTG GTTTCTGCGG 
GAAGGGAGTA TGTTTGTGGC CCTCTCGGCC TCCGTGTTCA GCCTCCTGGC CATCGCCATT 
GAGCGCTACA TCACCATGCT GAAGATGAAA CTCCACAATG GGAGCAACAG GTTCCGCTCC 
TTCCTGCTCA TCAGTGCCTG CTGGGTCATC TCCCTCATCC TGGGGGGCCT GCCTATCATG 
GGCTGGAACT GCATCAGCAC GCTGCCCAGC TGTTCTACTG TGCTGCCGCT CTACCACAAG 
CACTATATCC TCTTCTGCAC CACGGTCTTC ACTCTGCTCC TGCTCTCCAT TGTCATCCTG 
TACTGCAGGA TCTACTCCTT GGTCAGGACT CGCAGCCGCC GTCTGACCTT CCGCAAGAAC 
ATTTCCAAGG CCAGCCGCAG CTCTGAGAAG TCTCTGGCAC TGCTCAAGAC CGTGATTATT 
GTCCTGGGCG TCTTCATCGC CTGCTGGGCA CCACTCTTCA TCCTGCTCCT GCTCGACGTG 
GGCTGCAAGG TGAAGACCTG TGACATCCTC TTCAGAACGG AGTACTTCCT GGTGTTGGCT 
GTGCTCAACT CTGGCACCAA CCCCATCATT TACACTTTGT CCAACAAGGA GATGCGTCGG 
GCCTTCGTCC GGATCATGTC CTGCTGCAAG TGCCCCAGTA GAGACTCCGC GAGTAAATTC 
ACACGACCCA TCATCGCGGG CATGGAATTC AGCCGCAGTA AGTCAGACAA CTCCTCCCAC 
CCCCAGAAGG ACGATGGGGA CAACCCAGAG ACCATTATGT CTTCTGGAAA CGTCAACTCT 
TCTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840335.1
CDS	313..1461
Translation	MGSTRIPLVKALHSPVSDYVNYDIIVRHYNYTGKLKISADKDNGIKLISVVFILICCFIILENIFVLLTIWKTKKFHRPMYYFIGNLALSDLLAGVAYTANLLLSGATTYKLTPAQWFLREGSMFVALSASVFSLLAIAIERYITMLKMKLHNGSNRFRSFLLISACWVISLILGGLPIMGWNCISTLPSCSTVLPLYHKHYILFCTTVFTLLLLSIVILYCRIYSLVRTRSRRLTFRKNISKASRSSEKSLALLKTVIIVLGVFIACWAPLFILLLLDVGCKVKTCDILFRTEYFLVLAVLNSGTNPIIYTLSNKEMRRAFVRIMSCCKCPSRDSASKFTRPIIAGMEFSRSKSDNSSHPQKDDGDNPETIMSSGNVNSSS

Target ORF information:

RefSeq Version	XM_024984567.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sphingosine-1-phosphate receptor 1 (S1PR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984567.1

ORF Insert Sequence:

ATGGGGTCCA CCCGTATCCC GCTGGTCAAG GCCCTCCACA GCCCTGTCTC GGACTATGTC 
AACTACGACA TCATCGTCCG GCATTATAAC TACACGGGAA AGCTGAAGAT CAGCGCGGAC 
AAGGACAATG GCATTAAACT GATCTCAGTG GTGTTCATTC TCATCTGCTG CTTTATCATC 
CTAGAGAACA TTTTTGTTTT GCTGACCATC TGGAAAACCA AGAAGTTCCA CCGACCCATG 
TACTATTTTA TCGGCAATCT GGCCCTCTCA GACCTGTTGG CGGGAGTGGC CTACACAGCC 
AACCTGCTCT TGTCTGGGGC CACCACCTAC AAGCTAACCC CCGCCCAGTG GTTTCTGCGG 
GAAGGGAGTA TGTTTGTGGC CCTCTCGGCC TCCGTGTTCA GCCTCCTGGC CATCGCCATT 
GAGCGCTACA TCACCATGCT GAAGATGAAA CTCCACAATG GGAGCAACAG GTTCCGCTCC 
TTCCTGCTCA TCAGTGCCTG CTGGGTCATC TCCCTCATCC TGGGGGGCCT GCCTATCATG 
GGCTGGAACT GCATCAGCAC GCTGCCCAGC TGTTCTACTG TGCTGCCGCT CTACCACAAG 
CACTATATCC TCTTCTGCAC CACGGTCTTC ACTCTGCTCC TGCTCTCCAT TGTCATCCTG 
TACTGCAGGA TCTACTCCTT GGTCAGGACT CGCAGCCGCC GTCTGACCTT CCGCAAGAAC 
ATTTCCAAGG CCAGCCGCAG CTCTGAGAAG TCTCTGGCAC TGCTCAAGAC CGTGATTATT 
GTCCTGGGCG TCTTCATCGC CTGCTGGGCA CCACTCTTCA TCCTGCTCCT GCTCGACGTG 
GGCTGCAAGG TGAAGACCTG TGACATCCTC TTCAGAACGG AGTACTTCCT GGTGTTGGCT 
GTGCTCAACT CTGGCACCAA CCCCATCATT TACACTTTGT CCAACAAGGA GATGCGTCGG 
GCCTTCGTCC GGATCATGTC CTGCTGCAAG TGCCCCAGTA GAGACTCCGC GAGTAAATTC 
ACACGACCCA TCATCGCGGG CATGGAATTC AGCCGCAGTA AGTCAGACAA CTCCTCCCAC 
CCCCAGAAGG ACGATGGGGA CAACCCAGAG ACCATTATGT CTTCTGGAAA CGTCAACTCT 
TCTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multiple marker effects of single nucleotide polymorphisms in three genes, AKIRIN2, EDG1 and RPL27A, for marbling development in Japanese Black cattle.
Animal science journal = Nihon chikusan Gakkaiho85(3)193-7(2014 Mar)
Sukegawa S,Miyake T,Ibi T,Takahagi Y,Murakami H,Morimatsu F,Yamada T

Novel SNP in 5' flanking region of EDG1 associated with marbling in Japanese Black beef cattle.
Animal science journal = Nihon chikusan Gakkaiho80(4)486-9(2009 Aug)
Yamada T,Sasaki S,Sukegawa S,Miyake T,Fujita T,Kose H,Morita M,Takahagi Y,Murakami H,Morimatsu F,Sasaki Y

Association of single nucleotide polymorphisms in the endothelial differentiation sphingolipid G-protein-coupled receptor 1 gene with marbling in Japanese Black beef cattle.
Animal genetics40(2)209-16(2009 Apr)
Yamada T,Itoh M,Nishimura S,Taniguchi Y,Miyake T,Sasaki S,Yoshioka S,Fujita T,Shiga K,Morita M,Sasaki Y

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Polymorphisms in promoter regions of PDHB, SORBS1, and EDG1 genes showing marbling-associated expression changes.
Research communications in molecular pathology and pharmacology120-121(1-6)105-14(2007-2008)
Yamada T,Sasaki S,Sukegawa S,Muramatsu Y,Takahagi Y,Yoshioka S,Fujita T,Morita M,Murakami H,Morimatsu F,Sasaki Y

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S1PR1 cDNA ORF clone, Bos taurus(cattle)

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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