ASS1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASS1
Entrez Gene ID	280726
Full Name	argininosuccinate synthase 1
Synonyms	ASS,CN
General protein information	Preferred Names argininosuccinate synthase 1 Names argininosuccinate synthase arginino succinate synthetase argininosuccinate synthetase 1 citrulline--aspartate ligase citrullinemia mutant arginino succinate synthetase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173892.4	NP_776317.1	argininosuccinate synthase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ass1	NP_031520.1
Arabidopsis thaliana (thale cress)	AT4G24830	NP_194214.2
Homo sapiens (human)	ASS1	NP_446464.1
	LOC716168	XP_001106949.2
Canis lupus familiaris (dog)	ASS1	XP_005625292.1
Rattus norvegicus (Norway rat)	Ass1	NP_037289.1
Kluyveromyces lactis	KLLA0C04037g	XP_452377.1
Magnaporthe oryzae (rice blast fungus)	MGG_15868	XP_003715367.1
Oryza sativa (rice)	Os12g0235800	NP_001066459.1
Xenopus tropicalis (tropical clawed frog)	ass1	NP_001011212.1
Pan troglodytes (chimpanzee)	ASS1	XP_003951602.1
Drosophila melanogaster (fruit fly)	CG1315	NP_649674.1
Eremothecium gossypii	AGOS_AER230C	NP_985087.2
Bos taurus (cattle)	ASS1	NP_776317.1
Gallus gallus (chicken)	ASS1	NP_001013413.1
Danio rerio (zebrafish)	ass1	NP_001004603.1
Anopheles gambiae (African malaria mosquito)	ASSY_ANOGA	XP_311863.3
Saccharomyces cerevisiae (baker's yeast)	ARG1	NP_014583.2
Schizosaccharomyces pombe (fission yeast)	arg12	NP_595183.1
Neurospora crassa	NCU02639	XP_963976.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein kinase C? phosphorylates a novel argininosuccinate synthase site at serine 328 during calcium-dependent stimulation of endothelial nitric-oxide synthase in vascular endothelial cells.
Haines RJ, Corbin KD, Pendleton LC, Eichler DC
The Journal of biological chemistry287(31)26168-76(2012 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Phosphorylation of argininosuccinate synthase by protein kinase A.
Corbin KD, Pendleton LC, Solomonson LP, Eichler DC
Biochemical and biophysical research communications377(4)1042-6(2008 Dec)

Tumor necrosis factor-alpha reduces argininosuccinate synthase expression and nitric oxide production in aortic endothelial cells.
Goodwin BL, Pendleton LC, Levy MM, Solomonson LP, Eichler DC
American journal of physiology. Heart and circulatory physiology293(2)H1115-21(2007 Aug)

Regulation of endothelial argininosuccinate synthase expression and NO production by an upstream open reading frame.
Pendleton LC, Goodwin BL, Solomonson LP, Eichler DC
The Journal of biological chemistry280(25)24252-60(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

calcium-dependent phosphorylation of argininosuccinate synthase Ser-328 is mediated by PKCalpha
Title: Protein kinase C? phosphorylates a novel argininosuccinate synthase site at serine 328 during calcium-dependent stimulation of endothelial nitric-oxide synthase in vascular endothelial cells.

These results represent the first demonstration that vascular endothelial nitric oxide production can be regulated by dynamic argininosuccinate synthase phosphorylation.
Title: Phosphorylation of argininosuccinate synthase by protein kinase A.

In endothelial cells TNF-alpha coordinately downregulates nitric oxide synthase (eNOS) and argininosuccinate synthase expression, resulting in a severely impaired citrulline-NO cycle.
Title: Tumor necrosis factor-alpha reduces argininosuccinate synthase expression and nitric oxide production in aortic endothelial cells.

the function of the extended 5'-UTRs of AS mRNA was investigated
Title: Regulation of endothelial argininosuccinate synthase expression and NO production by an upstream open reading frame.

dose-dependent reduction of ASS as a result of siRNA treatment corresponded to diminished capacity to produce NO, despite saturating levels of arginine in the medium; reduced expression of ASS resulted in loss of viability due to apoptosis
Title: Argininosuccinate synthase expression is required to maintain nitric oxide production and cell viability in aortic endothelial cells.

The following ASS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44680	NM_173892.4 Latest version!	Bos taurus argininosuccinate synthase 1 (ASS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASS1 ( NM_173892.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776317.1 Bos taurus argininosuccinate synthase 1 (ASS1), mRNA.

Price & Availability↑

CloneID	OBa44680
Clone ID Related Accession (Same CDS sequence)	NM_173892.4
Accession Version	NM_173892.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	argininosuccinate synthase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC102474.1. On Aug 30, 2012 this sequence version replaced NM_173892.3. ##Evidence-Data-START## Transcript exon combination :: BC102474.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGGCA AAGGCTCCGT GGTTCTGGCC TACAGTGGGG GCCTGGACAC CTCCTGCATC 
CTCGTGTGGC TGAAGGAGCA AGGCTATGAC GTCATTGCCT ACCTGGCCAA CATCGGCCAG 
AAAGAAGACT TTGAGGAAGC CAGGAAGAAG GCGCTGAAGC TTGGGGCCAA AAAGGTGTTC 
ATTGAGGACA TCAGCAAGGA GTTTGTGGAG GAGTTCATCT GGCCGGCCAT CCAGTCCAGC 
GCACTGTACG AGGACCGATA CCTCCTGGGC ACCTCTCTCG CCAGGCCCTG CATCGCCCGC 
AAGCAGGTGG AGATCGCCCA GCGAGAAGGA GCCAAGTATG TGTCTCACGG CGCCACAGGA 
AAGGGGAACG ACCAGATCCG GTTTGAGCTC ACCTGCTACT CGCTGGCCCC ACAGATCAAG 
GTCATCGCTC CCTGGAGGAT GCCCGAGTTC TATAACCGCT TCCAGGGCCG CAACGATCTG 
ATGGAGTATG CGAAGCAACA TGGAATCCCC GTCCCAGTCA CCCCCAAGAA CCCGTGGAGC 
ATGGACGAGA ACCTGATGCA TATCAGCTAC GAGGCTGGAA TCCTGGAGAA CCCCAAGAAC 
CAAGCGCCTC CAGGCCTCTA CACAAAGACC CAGGACCCGG CCAAAGCCCC CAACAGCCCG 
GACATGCTCG AGATCGAGTT CAAGAAAGGG GTCCCCGTGA AGGTGACCAA CGTCGGGGAT 
GGCACCACCC ACAGCACAGC CTTGGAGCTT TTCCTGTACC TGAATGAAGT CGCTGGCAAG 
CACGGCGTGG GCCGCATCGA CATCGTGGAA AACCGCTTCA TCGGGATGAA GTCCCGGGGT 
ATCTACGAGA CCCCAGCGGG GACGATCCTT TACCACGCTC ATTTAGACAT CGAGGCCTTC 
ACCATGGACC GGGAAGTGCG CAAAATCAAG CAAGGCCTCG GCTTGAAATT CGCCGAGCTG 
GTCTACACGG GTTTCTGGCA CAGCCCCGAG TGTGAATTTG TCCGCCACTG CATTGCCAAG 
TCCCAGGAGC GCGTGGAAGG GAAAGTGCAG GTGTCCGTCT TCAAGGGCCA GGTGTACATC 
CTTGGCCGGG AGTCCCCACT GTCCCTCTAC AATGAGGAGC TCGTGAGCAT GAACGTGCAG 
GGAGACTACG AGCCGGTTGA TGCCACTGGT TTCATCAACA TCAATTCCCT CAGGCTGAAG 
GAATATCATC GCCTCCAGAA CAAGGTCACC GCCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776317.1
CDS	44..1282
Translation	MSGKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDISKEFVEEFIWPAIQSSALYEDRYLLGTSLARPCIARKQVEIAQREGAKYVSHGATGKGNDQIRFELTCYSLAPQIKVIAPWRMPEFYNRFQGRNDLMEYAKQHGIPVPVTPKNPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNSPDMLEIEFKKGVPVKVTNVGDGTTHSTALELFLYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIAKSQERVEGKVQVSVFKGQVYILGRESPLSLYNEELVSMNVQGDYEPVDATGFININSLRLKEYHRLQNKVTAK

Target ORF information:

RefSeq Version	NM_173892.4
Organism	Bos taurus(cattle)
Definition	Bos taurus argininosuccinate synthase 1 (ASS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173892.4

ORF Insert Sequence:

ATGTCCGGCA AAGGCTCCGT GGTTCTGGCC TACAGTGGGG GCCTGGACAC CTCCTGCATC 
CTCGTGTGGC TGAAGGAGCA AGGCTATGAC GTCATTGCCT ACCTGGCCAA CATCGGCCAG 
AAAGAAGACT TTGAGGAAGC CAGGAAGAAG GCGCTGAAGC TTGGGGCCAA AAAGGTGTTC 
ATTGAGGACA TCAGCAAGGA GTTTGTGGAG GAGTTCATCT GGCCGGCCAT CCAGTCCAGC 
GCACTGTACG AGGACCGATA CCTCCTGGGC ACCTCTCTCG CCAGGCCCTG CATCGCCCGC 
AAGCAGGTGG AGATCGCCCA GCGAGAAGGA GCCAAGTATG TGTCTCACGG CGCCACAGGA 
AAGGGGAACG ACCAGATCCG GTTTGAGCTC ACCTGCTACT CGCTGGCCCC ACAGATCAAG 
GTCATCGCTC CCTGGAGGAT GCCCGAGTTC TATAACCGCT TCCAGGGCCG CAACGATCTG 
ATGGAGTATG CGAAGCAACA TGGAATCCCC GTCCCAGTCA CCCCCAAGAA CCCGTGGAGC 
ATGGACGAGA ACCTGATGCA TATCAGCTAC GAGGCTGGAA TCCTGGAGAA CCCCAAGAAC 
CAAGCGCCTC CAGGCCTCTA CACAAAGACC CAGGACCCGG CCAAAGCCCC CAACAGCCCG 
GACATGCTCG AGATCGAGTT CAAGAAAGGG GTCCCCGTGA AGGTGACCAA CGTCGGGGAT 
GGCACCACCC ACAGCACAGC CTTGGAGCTT TTCCTGTACC TGAATGAAGT CGCTGGCAAG 
CACGGCGTGG GCCGCATCGA CATCGTGGAA AACCGCTTCA TCGGGATGAA GTCCCGGGGT 
ATCTACGAGA CCCCAGCGGG GACGATCCTT TACCACGCTC ATTTAGACAT CGAGGCCTTC 
ACCATGGACC GGGAAGTGCG CAAAATCAAG CAAGGCCTCG GCTTGAAATT CGCCGAGCTG 
GTCTACACGG GTTTCTGGCA CAGCCCCGAG TGTGAATTTG TCCGCCACTG CATTGCCAAG 
TCCCAGGAGC GCGTGGAAGG GAAAGTGCAG GTGTCCGTCT TCAAGGGCCA GGTGTACATC 
CTTGGCCGGG AGTCCCCACT GTCCCTCTAC AATGAGGAGC TCGTGAGCAT GAACGTGCAG 
GGAGACTACG AGCCGGTTGA TGCCACTGGT TTCATCAACA TCAATTCCCT CAGGCTGAAG 
GAATATCATC GCCTCCAGAA CAAGGTCACC GCCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein kinase C? phosphorylates a novel argininosuccinate synthase site at serine 328 during calcium-dependent stimulation of endothelial nitric-oxide synthase in vascular endothelial cells.
The Journal of biological chemistry287(31)26168-76(2012 Jul)
Haines RJ,Corbin KD,Pendleton LC,Eichler DC

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Phosphorylation of argininosuccinate synthase by protein kinase A.
Biochemical and biophysical research communications377(4)1042-6(2008 Dec)
Corbin KD,Pendleton LC,Solomonson LP,Eichler DC

Tumor necrosis factor-alpha reduces argininosuccinate synthase expression and nitric oxide production in aortic endothelial cells.
American journal of physiology. Heart and circulatory physiology293(2)H1115-21(2007 Aug)
Goodwin BL,Pendleton LC,Levy MM,Solomonson LP,Eichler DC

Regulation of endothelial argininosuccinate synthase expression and NO production by an upstream open reading frame.
The Journal of biological chemistry280(25)24252-60(2005 Jun)
Pendleton LC,Goodwin BL,Solomonson LP,Eichler DC

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ASS1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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