Ass1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ass1
Entrez Gene ID	25698
Full Name	argininosuccinate synthase 1
Synonyms	ASSA,Ass
General protein information	Preferred Names argininosuccinate synthase 1 Names argininosuccinate synthase argininosuccinate synthetase 1 arginosuccinate synthetase 1 citrulline--aspartate ligase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p12
Summary	catalyzes the conversion of ATP, L-citrulline, and L-aspartate to AMP, diphosphate, and L-argininosuccinate in arginine biosynthesis; may regulate nitric oxide production [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006233911.3	XP_006233973.1	argininosuccinate synthase isoform X1
NM_013157.3	NP_037289.1	argininosuccinate synthase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ass1	NP_031520.1
Arabidopsis thaliana (thale cress)	AT4G24830	NP_194214.2
Homo sapiens (human)	ASS1	NP_446464.1
	LOC716168	XP_001106949.2
Canis lupus familiaris (dog)	ASS1	XP_005625292.1
Rattus norvegicus (Norway rat)	Ass1	NP_037289.1
Kluyveromyces lactis	KLLA0C04037g	XP_452377.1
Magnaporthe oryzae (rice blast fungus)	MGG_15868	XP_003715367.1
Oryza sativa (rice)	Os12g0235800	NP_001066459.1
Xenopus tropicalis (tropical clawed frog)	ass1	NP_001011212.1
Pan troglodytes (chimpanzee)	ASS1	XP_003951602.1
Drosophila melanogaster (fruit fly)	CG1315	NP_649674.1
Eremothecium gossypii	AGOS_AER230C	NP_985087.2
Bos taurus (cattle)	ASS1	NP_776317.1
Gallus gallus (chicken)	ASS1	NP_001013413.1
Danio rerio (zebrafish)	ass1	NP_001004603.1
Anopheles gambiae (African malaria mosquito)	ASSY_ANOGA	XP_311863.3
Saccharomyces cerevisiae (baker's yeast)	ARG1	NP_014583.2
Schizosaccharomyces pombe (fission yeast)	arg12	NP_595183.1
Neurospora crassa	NCU02639	XP_963976.1

Gene Ontology
Bibliography

Related articles in PubMed

CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.
Lin R, Mo Y, Zha H, Qu Z, Xie P, Zhu ZJ, Xu Y, Xiong Y, Guan KL
Molecular cell68(1)198-209.e6(2017 Oct)

Kinetic mutations in argininosuccinate synthetase deficiency: characterisation and in vitro correction by substrate supplementation.
Diez-Fernandez C, Wellauer O, Gemperle C, R?fenacht V, Fingerhut R, H?berle J
Journal of medical genetics53(10)710-9(2016 Oct)

Argininosuccinate synthetase regulates hepatic AMPK linking protein catabolism and ureagenesis to hepatic lipid metabolism.
Madiraju AK, Alves T, Zhao X, Cline GW, Zhang D, Bhanot S, Samuel VT, Kibbey RG, Shulman GI
Proceedings of the National Academy of Sciences of the United States of America113(24)E3423-30(2016 06)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Toward an understanding of the protein interaction network of the human liver.
Wang J, Huo K, Ma L, Tang L, Li D, Huang X, Yuan Y, Li C, Wang W, Guan W, Chen H, Jin C, Wei J, Zhang W, Yang Y, Liu Q, Zhou Y, Zhang C, Wu Z, Xu W, Zhang Y, Liu T, Yu D, Zhang Y, Chen L, Zhu D, Zhong X, Kang L, Gan X, Yu X, Ma Q, Yan J, Zhou L, Liu Z, Zhu Y, Zhou T, He F, Yang X
Molecular systems biology7536(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these studies demonstrate that increased amino acid flux can activate AMPK through increased AMP generated by ASS, thus providing a novel link between protein catabolism, ureagenesis, and hepatic lipid metabolism.
Title: Argininosuccinate synthetase regulates hepatic AMPK linking protein catabolism and ureagenesis to hepatic lipid metabolism.

Local factors such as the NO produced by nNOS/ASS/ASL-positive neurons, could influence ASS/ASL-positive microglia cells avoiding or allowing the induction, in these cells, of iNOS.
Title: Activated microglia cells express argininosuccinate synthetase and argininosuccinate lyase in the rat brain after transient ischemia.

24 h after reperfusion, number of nNOS-positive neurons and tpercentage of those co-expressing ASS and ASL were significantly increased in the animals with a longer survival. 144 h after ischemia there was almost complete restore of these neurons.
Title: Transient ischemia increases neuronal nitric oxide synthase, argininosuccinate synthetase and argininosuccinate lyase co-expression in rat striatal neurons.

The latter, in turn, attenuates L-arg catabolism and urea production and, thereby, mitigates the fall in plasma L-arg.
Title: Effect of chronic renal failure on arginase and argininosuccinate synthetase expression.

Argininosuccinate synthetase has a role in preventing autotoxicity from nitric oxide overproduction
Title: Argininosuccinate synthetase is reversibly inactivated by S-nitrosylation in vitro and in vivo.

The following Ass1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ass1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02149	NM_013157.3 Latest version!	Rattus norvegicus argininosuccinate synthase 1 (Ass1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa02149	XM_006233911.3 Latest version!	Rattus norvegicus argininosuccinate synthase 1 (Ass1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ass1 ( NM_013157.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037289.1 Rattus norvegicus argininosuccinate synthase 1 (Ass1), mRNA.

Price & Availability↑

CloneID	ORa02149
Clone ID Related Accession (Same CDS sequence)	XM_006233911.3 , NM_013157.3
Accession Version	NM_013157.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	argininosuccinate synthase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X12459.1. On Jun 10, 2007 this sequence version replaced NM_013157.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M36708.1, X12459.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906354, SAMN01906355 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAGCA AGGGCTCTGT GGTTCTGGCC TACAGTGGTG GTCTGGACAC CTCCTGCATC 
CTCGTGTGGC TGAAGGAACA AGGCTATGAT GTCATCGCCT ACCTGGCCAA CATTGGCCAG 
AAGGAAGACT TTGAGGAAGC CAGGAAGAAG GCACTGAAGC TTGGGGCCAA AAAGGTGTTC 
ATTGAGGATG TAAGCAAGGA GTTTGTGGAA GAGTTCATCT GGCCTGCTGT CCAGTCCAGT 
GCACTCTATG AGGACCGCTA TCTCCTAGGC ACCTCTCTCG CCAGGCCTTG CATAGCTCGC 
AAACAAGTGG AAATTGCCCA GCGCGAAGGG GCCAAGTATG TGTCTCACGG CGCCACGGGG 
AAGGGCAATG ACCAGGTCCG CTTTGAGCTC ACCTGCTACT CGTTAGCACC CCAGATTAAG 
GTCATCGCCC CCTGGAGGAT GCCCGAGTTT TACAACCGGT TCAAGGGCCG AAATGATTTG 
ATGGAATACG CAAAGCAACA TGGAATCCCC ATCCCTGTCA CCCCCAAGAG CCCCTGGAGC 
ATGGATGAGA ACCTTATGCA CATCAGCTAC GAGGCTGGAA TCCTGGAAAA CCCCAAGAAC 
CAAGCACCTC CAGGTCTCTA CACAAAAACT CAGGACCCTG CCAAAGCACC CAACACCCCA 
GATGTCCTTG AGATAGAATT CAAAAAAGGG GTCCCTGTGA AGGTGACCAA CGTCAAAGAT 
GGCACTACCC ACAGCACATC CTTGGACCTC TTCATGTACC TGAATGAAGT TGCGGGCAAG 
CATGGAGTAG GGCGCATTGA CATCGTGGAG AACCGCTTCA TTGGAATGAA GTCCCGGGGT 
ATCTACGAGA CCCCAGCAGG GACCATCCTT TACCACGCTC ATTTAGACAT AGAGGCCTTC 
ACCATGGATC GGGAAGTACG CAAAATCAAG CAGGGCCTGG GCCTCAAATT CGCAGAGCTC 
GTATACACCG GTTTCTGGCA CAGCCCTGAA TGTGAATTTG TTCGCCACTG CATCGACAAG 
TCCCAGGAAC GGGTGGAAGG AAAGGTGCAG GTATCTGTCT TCAAGGGCCA GGTGTACATC 
CTTGGCCGGG AGTCTCCACT TTCACTATAC AATGAAGAGC TGGTGAGCAT GAACGTACAG 
GGTGACTATG AACCCATTGA TGCCACCGGC TTCATCAATA TCAACTCGCT CAGGCTGAAG 
GAGTACCATC GCCTTCAGAG CAAGGTCACC GCCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037289.1
CDS	15..1253
Translation	MSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSKEFVEEFIWPAVQSSALYEDRYLLGTSLARPCIARKQVEIAQREGAKYVSHGATGKGNDQVRFELTCYSLAPQIKVIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKSPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNTPDVLEIEFKKGVPVKVTNVKDGTTHSTSLDLFMYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIDKSQERVEGKVQVSVFKGQVYILGRESPLSLYNEELVSMNVQGDYEPIDATGFININSLRLKEYHRLQSKVTAK

Target ORF information:

RefSeq Version	NM_013157.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus argininosuccinate synthase 1 (Ass1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013157.3

ORF Insert Sequence:

ATGTCCAGCA AGGGCTCTGT GGTTCTGGCC TACAGTGGTG GTCTGGACAC CTCCTGCATC 
CTCGTGTGGC TGAAGGAACA AGGCTATGAT GTCATCGCCT ACCTGGCCAA CATTGGCCAG 
AAGGAAGACT TTGAGGAAGC CAGGAAGAAG GCACTGAAGC TTGGGGCCAA AAAGGTGTTC 
ATTGAGGATG TAAGCAAGGA GTTTGTGGAA GAGTTCATCT GGCCTGCTGT CCAGTCCAGT 
GCACTCTATG AGGACCGCTA TCTCCTAGGC ACCTCTCTCG CCAGGCCTTG CATAGCTCGC 
AAACAAGTGG AAATTGCCCA GCGCGAAGGG GCCAAGTATG TGTCTCACGG CGCCACGGGG 
AAGGGCAATG ACCAGGTCCG CTTTGAGCTC ACCTGCTACT CGTTAGCACC CCAGATTAAG 
GTCATCGCCC CCTGGAGGAT GCCCGAGTTT TACAACCGGT TCAAGGGCCG AAATGATTTG 
ATGGAATACG CAAAGCAACA TGGAATCCCC ATCCCTGTCA CCCCCAAGAG CCCCTGGAGC 
ATGGATGAGA ACCTTATGCA CATCAGCTAC GAGGCTGGAA TCCTGGAAAA CCCCAAGAAC 
CAAGCACCTC CAGGTCTCTA CACAAAAACT CAGGACCCTG CCAAAGCACC CAACACCCCA 
GATGTCCTTG AGATAGAATT CAAAAAAGGG GTCCCTGTGA AGGTGACCAA CGTCAAAGAT 
GGCACTACCC ACAGCACATC CTTGGACCTC TTCATGTACC TGAATGAAGT TGCGGGCAAG 
CATGGAGTAG GGCGCATTGA CATCGTGGAG AACCGCTTCA TTGGAATGAA GTCCCGGGGT 
ATCTACGAGA CCCCAGCAGG GACCATCCTT TACCACGCTC ATTTAGACAT AGAGGCCTTC 
ACCATGGATC GGGAAGTACG CAAAATCAAG CAGGGCCTGG GCCTCAAATT CGCAGAGCTC 
GTATACACCG GTTTCTGGCA CAGCCCTGAA TGTGAATTTG TTCGCCACTG CATCGACAAG 
TCCCAGGAAC GGGTGGAAGG AAAGGTGCAG GTATCTGTCT TCAAGGGCCA GGTGTACATC 
CTTGGCCGGG AGTCTCCACT TTCACTATAC AATGAAGAGC TGGTGAGCAT GAACGTACAG 
GGTGACTATG AACCCATTGA TGCCACCGGC TTCATCAATA TCAACTCGCT CAGGCTGAAG 
GAGTACCATC GCCTTCAGAG CAAGGTCACC GCCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ass1 ( XM_006233911.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233973.1 Rattus norvegicus argininosuccinate synthase 1 (Ass1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02149
Clone ID Related Accession (Same CDS sequence)	XM_006233911.3 , NM_013157.3
Accession Version	XM_006233911.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	argininosuccinate synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233911.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AGGGCTCTGT GGTTCTGGCC TACAGTGGTG GTCTGGACAC CTCCTGCATC 
CTCGTGTGGC TGAAGGAACA AGGCTATGAT GTCATCGCCT ACCTGGCCAA CATTGGCCAG 
AAGGAAGACT TTGAGGAAGC CAGGAAGAAG GCACTGAAGC TTGGGGCCAA AAAGGTGTTC 
ATTGAGGATG TAAGCAAGGA GTTTGTGGAA GAGTTCATCT GGCCTGCTGT CCAGTCCAGT 
GCACTCTATG AGGACCGCTA TCTCCTAGGC ACCTCTCTCG CCAGGCCTTG CATAGCTCGC 
AAACAAGTGG AAATTGCCCA GCGCGAAGGG GCCAAGTATG TGTCTCACGG CGCCACGGGG 
AAGGGCAATG ACCAGGTCCG CTTTGAGCTC ACCTGCTACT CGTTAGCACC CCAGATTAAG 
GTCATCGCCC CCTGGAGGAT GCCCGAGTTT TACAACCGGT TCAAGGGCCG AAATGATTTG 
ATGGAATACG CAAAGCAACA TGGAATCCCC ATCCCTGTCA CCCCCAAGAG CCCCTGGAGC 
ATGGATGAGA ACCTTATGCA CATCAGCTAC GAGGCTGGAA TCCTGGAAAA CCCCAAGAAC 
CAAGCACCTC CAGGTCTCTA CACAAAAACT CAGGACCCTG CCAAAGCACC CAACACCCCA 
GATGTCCTTG AGATAGAATT CAAAAAAGGG GTCCCTGTGA AGGTGACCAA CGTCAAAGAT 
GGCACTACCC ACAGCACATC CTTGGACCTC TTCATGTACC TGAATGAAGT TGCGGGCAAG 
CATGGAGTAG GGCGCATTGA CATCGTGGAG AACCGCTTCA TTGGAATGAA GTCCCGGGGT 
ATCTACGAGA CCCCAGCAGG GACCATCCTT TACCACGCTC ATTTAGACAT AGAGGCCTTC 
ACCATGGATC GGGAAGTACG CAAAATCAAG CAGGGCCTGG GCCTCAAATT CGCAGAGCTC 
GTATACACCG GTTTCTGGCA CAGCCCTGAA TGTGAATTTG TTCGCCACTG CATCGACAAG 
TCCCAGGAAC GGGTGGAAGG AAAGGTGCAG GTATCTGTCT TCAAGGGCCA GGTGTACATC 
CTTGGCCGGG AGTCTCCACT TTCACTATAC AATGAAGAGC TGGTGAGCAT GAACGTACAG 
GGTGACTATG AACCCATTGA TGCCACCGGC TTCATCAATA TCAACTCGCT CAGGCTGAAG 
GAGTACCATC GCCTTCAGAG CAAGGTCACC GCCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233973.1
CDS	175..1413
Translation	MSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSKEFVEEFIWPAVQSSALYEDRYLLGTSLARPCIARKQVEIAQREGAKYVSHGATGKGNDQVRFELTCYSLAPQIKVIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKSPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNTPDVLEIEFKKGVPVKVTNVKDGTTHSTSLDLFMYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIDKSQERVEGKVQVSVFKGQVYILGRESPLSLYNEELVSMNVQGDYEPIDATGFININSLRLKEYHRLQSKVTAK

Target ORF information:

RefSeq Version	XM_006233911.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus argininosuccinate synthase 1 (Ass1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233911.3

ORF Insert Sequence:

ATGTCCAGCA AGGGCTCTGT GGTTCTGGCC TACAGTGGTG GTCTGGACAC CTCCTGCATC 
CTCGTGTGGC TGAAGGAACA AGGCTATGAT GTCATCGCCT ACCTGGCCAA CATTGGCCAG 
AAGGAAGACT TTGAGGAAGC CAGGAAGAAG GCACTGAAGC TTGGGGCCAA AAAGGTGTTC 
ATTGAGGATG TAAGCAAGGA GTTTGTGGAA GAGTTCATCT GGCCTGCTGT CCAGTCCAGT 
GCACTCTATG AGGACCGCTA TCTCCTAGGC ACCTCTCTCG CCAGGCCTTG CATAGCTCGC 
AAACAAGTGG AAATTGCCCA GCGCGAAGGG GCCAAGTATG TGTCTCACGG CGCCACGGGG 
AAGGGCAATG ACCAGGTCCG CTTTGAGCTC ACCTGCTACT CGTTAGCACC CCAGATTAAG 
GTCATCGCCC CCTGGAGGAT GCCCGAGTTT TACAACCGGT TCAAGGGCCG AAATGATTTG 
ATGGAATACG CAAAGCAACA TGGAATCCCC ATCCCTGTCA CCCCCAAGAG CCCCTGGAGC 
ATGGATGAGA ACCTTATGCA CATCAGCTAC GAGGCTGGAA TCCTGGAAAA CCCCAAGAAC 
CAAGCACCTC CAGGTCTCTA CACAAAAACT CAGGACCCTG CCAAAGCACC CAACACCCCA 
GATGTCCTTG AGATAGAATT CAAAAAAGGG GTCCCTGTGA AGGTGACCAA CGTCAAAGAT 
GGCACTACCC ACAGCACATC CTTGGACCTC TTCATGTACC TGAATGAAGT TGCGGGCAAG 
CATGGAGTAG GGCGCATTGA CATCGTGGAG AACCGCTTCA TTGGAATGAA GTCCCGGGGT 
ATCTACGAGA CCCCAGCAGG GACCATCCTT TACCACGCTC ATTTAGACAT AGAGGCCTTC 
ACCATGGATC GGGAAGTACG CAAAATCAAG CAGGGCCTGG GCCTCAAATT CGCAGAGCTC 
GTATACACCG GTTTCTGGCA CAGCCCTGAA TGTGAATTTG TTCGCCACTG CATCGACAAG 
TCCCAGGAAC GGGTGGAAGG AAAGGTGCAG GTATCTGTCT TCAAGGGCCA GGTGTACATC 
CTTGGCCGGG AGTCTCCACT TTCACTATAC AATGAAGAGC TGGTGAGCAT GAACGTACAG 
GGTGACTATG AACCCATTGA TGCCACCGGC TTCATCAATA TCAACTCGCT CAGGCTGAAG 
GAGTACCATC GCCTTCAGAG CAAGGTCACC GCCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.
Molecular cell68(1)198-209.e6(2017 Oct)
Lin R,Mo Y,Zha H,Qu Z,Xie P,Zhu ZJ,Xu Y,Xiong Y,Guan KL

Kinetic mutations in argininosuccinate synthetase deficiency: characterisation and in vitro correction by substrate supplementation.
Journal of medical genetics53(10)710-9(2016 Oct)
Diez-Fernandez C,Wellauer O,Gemperle C,R?fenacht V,Fingerhut R,H?berle J

Argininosuccinate synthetase regulates hepatic AMPK linking protein catabolism and ureagenesis to hepatic lipid metabolism.
Proceedings of the National Academy of Sciences of the United States of America113(24)E3423-30(2016 06)
Madiraju AK,Alves T,Zhao X,Cline GW,Zhang D,Bhanot S,Samuel VT,Kibbey RG,Shulman GI

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Toward an understanding of the protein interaction network of the human liver.
Molecular systems biology7536(2011 Oct)
Wang J,Huo K,Ma L,Tang L,Li D,Huang X,Yuan Y,Li C,Wang W,Guan W,Chen H,Jin C,Wei J,Zhang W,Yang Y,Liu Q,Zhou Y,Zhang C,Wu Z,Xu W,Zhang Y,Liu T,Yu D,Zhang Y,Chen L,Zhu D,Zhong X,Kang L,Gan X,Yu X,Ma Q,Yan J,Zhou L,Liu Z,Zhu Y,Zhou T,He F,Yang X

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Ass1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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