LOC107131266 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107131266
Entrez Gene ID	107131266
Full Name	E3 ubiquitin-protein ligase HECW1-like
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015463084.1	XP_015318570.1	E3 ubiquitin-protein ligase HECW1-like isoform X3
XM_015463083.1	XP_015318569.1	E3 ubiquitin-protein ligase HECW1-like isoform X2
XM_015463082.1	XP_015318568.1	E3 ubiquitin-protein ligase HECW1-like isoform X1
XM_015470316.1	XP_015325802.1	E3 ubiquitin-protein ligase HECW1-like isoform X1
XM_015470318.1	XP_015325804.1	E3 ubiquitin-protein ligase HECW1-like isoform X2
XM_015470322.1	XP_015325808.1	E3 ubiquitin-protein ligase HECW1-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC107131266 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107131266 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa18736	XM_015470316.1 Latest version!	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa18736	XM_015463082.1 Latest version!	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa48883	XM_015470322.1 Latest version!	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa48883	XM_015463084.1 Latest version!	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa89755	XM_015463083.1 Latest version!	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa89755	XM_015470318.1 Latest version!	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107131266 ( XM_015463082.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318568.1 Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa18736
Clone ID Related Accession (Same CDS sequence)	XM_015463082.1 , XM_015470316.1
Accession Version	XM_015463082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HECW1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644732.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTCTT AGGCCTGGCC 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AATCGACGCC GGTGCAAGGA GCCGCTCCGC 
CACAGCTACA ATCCAGGCCA GTACCACAGT GTGGCCATCA GGACTGGCCC CCATGGTGCC 
GTCACCATCC CCCGCTCCAC CAGTGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACA 
CTCATGGTCA GCAGTTCCTA CTACTCCATA GGACACTCGC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGGGAC TGGATCGGCA TGTACCTCAT TGATGAGGTC 
TTGTCGGAAA ACTTCCTGGA CTATAAGAAC CGTGGAGTCA ATGGCTCTCA TCGGGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTAAG TGGAGCCTTG CGAGCCACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTGCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTTCCTGCCC TCCCCCACCA TGGACAGGAG AGGCGATCCA AAATCATTGG CAACACTGTG 
AACCCCATCT GGCAGGCTGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCAATGC CCGTTCAAAG ACTCCTGGAA AGACATGCCA TAGGGGACAG GGTGGTCAGC 
TACACCCTTG GCCGTAGGCT TCCAACAGAT CACGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCTGCGGAAA TTCAGGACAG CCCCATGAAT AACCTGGTGG CAAGCAGCTG TGGGGAGCCC 
CCGTGTGATG CACCAGAGCC CTCAGGGACC CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC 
ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG GAGCTTACCA GTCTGGTTGA GGCAGCGGCA 
GGTACCACGG AGGCAGGAGA TGATGATGGC ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG 
GAGCTGCTGG CCCCTGGGCC AGAGGACACA GAGCCCGCCC TAAGTGCAGA AGAACTGGCC 
GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC 
CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA 
CAGGAGGAGG AGAAGGAGCA GATGGAGGAG GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG 
GGGGAGGACA GGCTGCACCT GCGGGCCTCG GTGAAGAGGA AAACCAGGCC GTGCTCCCTG 
CCTGTGTCCG AGCTAGAGAC GGTGATCGCG TCCGCTTGCG GTGAGCCGGA GACACCGCGG 
ACCCACTACA TCCGCATCCA CACGCTGCTG CACAGCCTGC CCTCGGCTCA GGGCGGCAGC 
GCGGACGACG ACGACGACGG TGCCGAGGAG GAGGCCACCC TCAAGGATGC CTCGGAGAAG 
GATGCGCTCA GTGAGATGGA CACGGTCGCC GCGGAGCCTC CTCCCCTGGA AGAGGATGGA 
GAGGCCCCCG AGGGGGCCAC TCCAGGCACG GCTCCCCTCG GCCACTCGGG CTTGGCTAAC 
GGCTCAGCCC CGGATGGCGA CGCGCTCCCC AGCACGGGGA GCGAGAGCGA CTCCAGCCCG 
CGGCAGGGTG GAGACCACAG CTGCTGCGAC GCATCCTGCT GCAGCCCCTC CTGCTACAGC 
TCCTCGTGCT ACAGCAGCTC CTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAGTAGGT TTGCCAGCCA CACCCGCTTC TCCTCTGTGG ACAGTGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA GGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG 
CCCGACTCAG TGCAGAGCCC AGAGCTGGAC CAGGAGTCGG CCAACGGTGC AGGGCCATGG 
CAAGACGAGC TGGCCGCCCC CGATGGGAGC ATGGCCAGAA CCATGGAAGG TCTGGAGTCC 
CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CTTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAGTAAGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318568.1
CDS	244..2727
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRHSYNPGQYHSVAIRTGPHGAVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSPMNNLVASSCGEPPCDAPEPSGTPKPEQPSQGSTVGPPEIEGLELTSLVEAAAGTTEAGDDDGIVSVGPEGAGELLAPGPEDTEPALSAEELAERLDLGGEVALPGMLEDGQAPSSPGEPVAEERATESRAGGQEEEKEQMEEEEGDVSALGQGEDRLHLRASVKRKTRPCSLPVSELETVIASACGEPETPRTHYIRIHTLLHSLPSAQGGSADDDDDGAEEEATLKDASEKDALSEMDTVAAEPPPLEEDGEAPEGATPGTAPLGHSGLANGSAPDGDALPSTGSESDSSPRQGGDHSCCDASCCSPSCYSSSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDQESANGAGPWQDELAAPDGSMARTMEGLESPVAGPSSRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPSKL

Target ORF information:

RefSeq Version	XM_015463082.1
Organism	Bos taurus(cattle)
Definition	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463082.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTCTT AGGCCTGGCC 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AATCGACGCC GGTGCAAGGA GCCGCTCCGC 
CACAGCTACA ATCCAGGCCA GTACCACAGT GTGGCCATCA GGACTGGCCC CCATGGTGCC 
GTCACCATCC CCCGCTCCAC CAGTGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACA 
CTCATGGTCA GCAGTTCCTA CTACTCCATA GGACACTCGC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGGGAC TGGATCGGCA TGTACCTCAT TGATGAGGTC 
TTGTCGGAAA ACTTCCTGGA CTATAAGAAC CGTGGAGTCA ATGGCTCTCA TCGGGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTAAG TGGAGCCTTG CGAGCCACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTGCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTTCCTGCCC TCCCCCACCA TGGACAGGAG AGGCGATCCA AAATCATTGG CAACACTGTG 
AACCCCATCT GGCAGGCTGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCAATGC CCGTTCAAAG ACTCCTGGAA AGACATGCCA TAGGGGACAG GGTGGTCAGC 
TACACCCTTG GCCGTAGGCT TCCAACAGAT CACGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCTGCGGAAA TTCAGGACAG CCCCATGAAT AACCTGGTGG CAAGCAGCTG TGGGGAGCCC 
CCGTGTGATG CACCAGAGCC CTCAGGGACC CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC 
ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG GAGCTTACCA GTCTGGTTGA GGCAGCGGCA 
GGTACCACGG AGGCAGGAGA TGATGATGGC ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG 
GAGCTGCTGG CCCCTGGGCC AGAGGACACA GAGCCCGCCC TAAGTGCAGA AGAACTGGCC 
GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC 
CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA 
CAGGAGGAGG AGAAGGAGCA GATGGAGGAG GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG 
GGGGAGGACA GGCTGCACCT GCGGGCCTCG GTGAAGAGGA AAACCAGGCC GTGCTCCCTG 
CCTGTGTCCG AGCTAGAGAC GGTGATCGCG TCCGCTTGCG GTGAGCCGGA GACACCGCGG 
ACCCACTACA TCCGCATCCA CACGCTGCTG CACAGCCTGC CCTCGGCTCA GGGCGGCAGC 
GCGGACGACG ACGACGACGG TGCCGAGGAG GAGGCCACCC TCAAGGATGC CTCGGAGAAG 
GATGCGCTCA GTGAGATGGA CACGGTCGCC GCGGAGCCTC CTCCCCTGGA AGAGGATGGA 
GAGGCCCCCG AGGGGGCCAC TCCAGGCACG GCTCCCCTCG GCCACTCGGG CTTGGCTAAC 
GGCTCAGCCC CGGATGGCGA CGCGCTCCCC AGCACGGGGA GCGAGAGCGA CTCCAGCCCG 
CGGCAGGGTG GAGACCACAG CTGCTGCGAC GCATCCTGCT GCAGCCCCTC CTGCTACAGC 
TCCTCGTGCT ACAGCAGCTC CTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAGTAGGT TTGCCAGCCA CACCCGCTTC TCCTCTGTGG ACAGTGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA GGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG 
CCCGACTCAG TGCAGAGCCC AGAGCTGGAC CAGGAGTCGG CCAACGGTGC AGGGCCATGG 
CAAGACGAGC TGGCCGCCCC CGATGGGAGC ATGGCCAGAA CCATGGAAGG TCTGGAGTCC 
CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CTTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAGTAAGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107131266 ( XM_015470316.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325802.1 Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa18736
Clone ID Related Accession (Same CDS sequence)	XM_015463082.1 , XM_015470316.1
Accession Version	XM_015470316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HECW1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTCTT AGGCCTGGCC 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AATCGACGCC GGTGCAAGGA GCCGCTCCGC 
CACAGCTACA ATCCAGGCCA GTACCACAGT GTGGCCATCA GGACTGGCCC CCATGGTGCC 
GTCACCATCC CCCGCTCCAC CAGTGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACA 
CTCATGGTCA GCAGTTCCTA CTACTCCATA GGACACTCGC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGGGAC TGGATCGGCA TGTACCTCAT TGATGAGGTC 
TTGTCGGAAA ACTTCCTGGA CTATAAGAAC CGTGGAGTCA ATGGCTCTCA TCGGGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTAAG TGGAGCCTTG CGAGCCACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTGCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTTCCTGCCC TCCCCCACCA TGGACAGGAG AGGCGATCCA AAATCATTGG CAACACTGTG 
AACCCCATCT GGCAGGCTGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCAATGC CCGTTCAAAG ACTCCTGGAA AGACATGCCA TAGGGGACAG GGTGGTCAGC 
TACACCCTTG GCCGTAGGCT TCCAACAGAT CACGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCTGCGGAAA TTCAGGACAG CCCCATGAAT AACCTGGTGG CAAGCAGCTG TGGGGAGCCC 
CCGTGTGATG CACCAGAGCC CTCAGGGACC CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC 
ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG GAGCTTACCA GTCTGGTTGA GGCAGCGGCA 
GGTACCACGG AGGCAGGAGA TGATGATGGC ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG 
GAGCTGCTGG CCCCTGGGCC AGAGGACACA GAGCCCGCCC TAAGTGCAGA AGAACTGGCC 
GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC 
CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA 
CAGGAGGAGG AGAAGGAGCA GATGGAGGAG GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG 
GGGGAGGACA GGCTGCACCT GCGGGCCTCG GTGAAGAGGA AAACCAGGCC GTGCTCCCTG 
CCTGTGTCCG AGCTAGAGAC GGTGATCGCG TCCGCTTGCG GTGAGCCGGA GACACCGCGG 
ACCCACTACA TCCGCATCCA CACGCTGCTG CACAGCCTGC CCTCGGCTCA GGGCGGCAGC 
GCGGACGACG ACGACGACGG TGCCGAGGAG GAGGCCACCC TCAAGGATGC CTCGGAGAAG 
GATGCGCTCA GTGAGATGGA CACGGTCGCC GCGGAGCCTC CTCCCCTGGA AGAGGATGGA 
GAGGCCCCCG AGGGGGCCAC TCCAGGCACG GCTCCCCTCG GCCACTCGGG CTTGGCTAAC 
GGCTCAGCCC CGGATGGCGA CGCGCTCCCC AGCACGGGGA GCGAGAGCGA CTCCAGCCCG 
CGGCAGGGTG GAGACCACAG CTGCTGCGAC GCATCCTGCT GCAGCCCCTC CTGCTACAGC 
TCCTCGTGCT ACAGCAGCTC CTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAGTAGGT TTGCCAGCCA CACCCGCTTC TCCTCTGTGG ACAGTGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA GGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG 
CCCGACTCAG TGCAGAGCCC AGAGCTGGAC CAGGAGTCGG CCAACGGTGC AGGGCCATGG 
CAAGACGAGC TGGCCGCCCC CGATGGGAGC ATGGCCAGAA CCATGGAAGG TCTGGAGTCC 
CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CTTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAGTAAGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325802.1
CDS	244..2727
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRHSYNPGQYHSVAIRTGPHGAVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSPMNNLVASSCGEPPCDAPEPSGTPKPEQPSQGSTVGPPEIEGLELTSLVEAAAGTTEAGDDDGIVSVGPEGAGELLAPGPEDTEPALSAEELAERLDLGGEVALPGMLEDGQAPSSPGEPVAEERATESRAGGQEEEKEQMEEEEGDVSALGQGEDRLHLRASVKRKTRPCSLPVSELETVIASACGEPETPRTHYIRIHTLLHSLPSAQGGSADDDDDGAEEEATLKDASEKDALSEMDTVAAEPPPLEEDGEAPEGATPGTAPLGHSGLANGSAPDGDALPSTGSESDSSPRQGGDHSCCDASCCSPSCYSSSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDQESANGAGPWQDELAAPDGSMARTMEGLESPVAGPSSRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPSKL

Target ORF information:

RefSeq Version	XM_015470316.1
Organism	Bos taurus(cattle)
Definition	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470316.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTCTT AGGCCTGGCC 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AATCGACGCC GGTGCAAGGA GCCGCTCCGC 
CACAGCTACA ATCCAGGCCA GTACCACAGT GTGGCCATCA GGACTGGCCC CCATGGTGCC 
GTCACCATCC CCCGCTCCAC CAGTGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACA 
CTCATGGTCA GCAGTTCCTA CTACTCCATA GGACACTCGC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGGGAC TGGATCGGCA TGTACCTCAT TGATGAGGTC 
TTGTCGGAAA ACTTCCTGGA CTATAAGAAC CGTGGAGTCA ATGGCTCTCA TCGGGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTAAG TGGAGCCTTG CGAGCCACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTGCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTTCCTGCCC TCCCCCACCA TGGACAGGAG AGGCGATCCA AAATCATTGG CAACACTGTG 
AACCCCATCT GGCAGGCTGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCAATGC CCGTTCAAAG ACTCCTGGAA AGACATGCCA TAGGGGACAG GGTGGTCAGC 
TACACCCTTG GCCGTAGGCT TCCAACAGAT CACGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCTGCGGAAA TTCAGGACAG CCCCATGAAT AACCTGGTGG CAAGCAGCTG TGGGGAGCCC 
CCGTGTGATG CACCAGAGCC CTCAGGGACC CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC 
ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG GAGCTTACCA GTCTGGTTGA GGCAGCGGCA 
GGTACCACGG AGGCAGGAGA TGATGATGGC ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG 
GAGCTGCTGG CCCCTGGGCC AGAGGACACA GAGCCCGCCC TAAGTGCAGA AGAACTGGCC 
GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC 
CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA 
CAGGAGGAGG AGAAGGAGCA GATGGAGGAG GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG 
GGGGAGGACA GGCTGCACCT GCGGGCCTCG GTGAAGAGGA AAACCAGGCC GTGCTCCCTG 
CCTGTGTCCG AGCTAGAGAC GGTGATCGCG TCCGCTTGCG GTGAGCCGGA GACACCGCGG 
ACCCACTACA TCCGCATCCA CACGCTGCTG CACAGCCTGC CCTCGGCTCA GGGCGGCAGC 
GCGGACGACG ACGACGACGG TGCCGAGGAG GAGGCCACCC TCAAGGATGC CTCGGAGAAG 
GATGCGCTCA GTGAGATGGA CACGGTCGCC GCGGAGCCTC CTCCCCTGGA AGAGGATGGA 
GAGGCCCCCG AGGGGGCCAC TCCAGGCACG GCTCCCCTCG GCCACTCGGG CTTGGCTAAC 
GGCTCAGCCC CGGATGGCGA CGCGCTCCCC AGCACGGGGA GCGAGAGCGA CTCCAGCCCG 
CGGCAGGGTG GAGACCACAG CTGCTGCGAC GCATCCTGCT GCAGCCCCTC CTGCTACAGC 
TCCTCGTGCT ACAGCAGCTC CTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAGTAGGT TTGCCAGCCA CACCCGCTTC TCCTCTGTGG ACAGTGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA GGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG 
CCCGACTCAG TGCAGAGCCC AGAGCTGGAC CAGGAGTCGG CCAACGGTGC AGGGCCATGG 
CAAGACGAGC TGGCCGCCCC CGATGGGAGC ATGGCCAGAA CCATGGAAGG TCTGGAGTCC 
CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CTTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAGTAAGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107131266 ( XM_015463084.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318570.1 Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa48883
Clone ID Related Accession (Same CDS sequence)	XM_015463084.1 , XM_015470322.1
Accession Version	XM_015463084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HECW1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644732.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TGTCAGAACC TAAAGAGGAC TGCCTTTCAG AGGAGATTTT TAAAAGCATT 
GGCTCTGATG AGACCGTGCA AGGGCAAGGA AGTCGGAGGC TGATCAGCTT TTCTCTCTCA 
GATTTCCAGG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTACCTGAAG 
ATTTCTATTC AGCCTGGGAA GCACAGCATC TTTCCTGCCC TCCCCCACCA TGGACAGGAG 
AGGCGATCCA AAATCATTGG CAACACTGTG AACCCCATCT GGCAGGCTGA GCAATTCAGC 
TTTGTGTCCT TGCCCACTGA TGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT TTTGGGAAAG CTGTCAATGC CCGTTCAAAG ACTCCTGGAA 
AGACATGCCA TAGGGGACAG GGTGGTCAGC TACACCCTTG GCCGTAGGCT TCCAACAGAT 
CACGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGATGAT 
GAGGAGATTT CCCTGAGTAC CGAGCCTGAG TCTGCGGAAA TTCAGGACAG CCCCATGAAT 
AACCTGGTGG CAAGCAGCTG TGGGGAGCCC CCGTGTGATG CACCAGAGCC CTCAGGGACC 
CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG 
GAGCTTACCA GTCTGGTTGA GGCAGCGGCA GGTACCACGG AGGCAGGAGA TGATGATGGC 
ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG GAGCTGCTGG CCCCTGGGCC AGAGGACACA 
GAGCCCGCCC TAAGTGCAGA AGAACTGGCC GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC 
CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG 
GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA CAGGAGGAGG AGAAGGAGCA GATGGAGGAG 
GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG GGGGAGGACA GGCTGCACCT GCGGGCCTCG 
GTGAAGAGGA AAACCAGGCC GTGCTCCCTG CCTGTGTCCG AGCTAGAGAC GGTGATCGCG 
TCCGCTTGCG GTGAGCCGGA GACACCGCGG ACCCACTACA TCCGCATCCA CACGCTGCTG 
CACAGCCTGC CCTCGGCTCA GGGCGGCAGC GCGGACGACG ACGACGACGG TGCCGAGGAG 
GAGGCCACCC TCAAGGATGC CTCGGAGAAG GATGCGCTCA GTGAGATGGA CACGGTCGCC 
GCGGAGCCTC CTCCCCTGGA AGAGGATGGA GAGGCCCCCG AGGGGGCCAC TCCAGGCACG 
GCTCCCCTCG GCCACTCGGG CTTGGCTAAC GGCTCAGCCC CGGATGGCGA CGCGCTCCCC 
AGCACGGGGA GCGAGAGCGA CTCCAGCCCG CGGCAGGGTG GAGACCACAG CTGCTGCGAC 
GCATCCTGCT GCAGCCCCTC CTGCTACAGC TCCTCGTGCT ACAGCAGCTC CTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAGTAGGT TTGCCAGCCA CACCCGCTTC 
TCCTCTGTGG ACAGTGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA GGACGACGAG 
GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG CCCGACTCAG TGCAGAGCCC AGAGCTGGAC 
CAGGAGTCGG CCAACGGTGC AGGGCCATGG CAAGACGAGC TGGCCGCCCC CGATGGGAGC 
ATGGCCAGAA CCATGGAAGG TCTGGAGTCC CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CTTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAGTAAGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318570.1
CDS	1597..3570
Translation	MAALSEPKEDCLSEEIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSPMNNLVASSCGEPPCDAPEPSGTPKPEQPSQGSTVGPPEIEGLELTSLVEAAAGTTEAGDDDGIVSVGPEGAGELLAPGPEDTEPALSAEELAERLDLGGEVALPGMLEDGQAPSSPGEPVAEERATESRAGGQEEEKEQMEEEEGDVSALGQGEDRLHLRASVKRKTRPCSLPVSELETVIASACGEPETPRTHYIRIHTLLHSLPSAQGGSADDDDDGAEEEATLKDASEKDALSEMDTVAAEPPPLEEDGEAPEGATPGTAPLGHSGLANGSAPDGDALPSTGSESDSSPRQGGDHSCCDASCCSPSCYSSSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDQESANGAGPWQDELAAPDGSMARTMEGLESPVAGPSSRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPSKL

Target ORF information:

RefSeq Version	XM_015463084.1
Organism	Bos taurus(cattle)
Definition	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463084.1

ORF Insert Sequence:

ATGGCTGCTC TGTCAGAACC TAAAGAGGAC TGCCTTTCAG AGGAGATTTT TAAAAGCATT 
GGCTCTGATG AGACCGTGCA AGGGCAAGGA AGTCGGAGGC TGATCAGCTT TTCTCTCTCA 
GATTTCCAGG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTACCTGAAG 
ATTTCTATTC AGCCTGGGAA GCACAGCATC TTTCCTGCCC TCCCCCACCA TGGACAGGAG 
AGGCGATCCA AAATCATTGG CAACACTGTG AACCCCATCT GGCAGGCTGA GCAATTCAGC 
TTTGTGTCCT TGCCCACTGA TGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT TTTGGGAAAG CTGTCAATGC CCGTTCAAAG ACTCCTGGAA 
AGACATGCCA TAGGGGACAG GGTGGTCAGC TACACCCTTG GCCGTAGGCT TCCAACAGAT 
CACGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGATGAT 
GAGGAGATTT CCCTGAGTAC CGAGCCTGAG TCTGCGGAAA TTCAGGACAG CCCCATGAAT 
AACCTGGTGG CAAGCAGCTG TGGGGAGCCC CCGTGTGATG CACCAGAGCC CTCAGGGACC 
CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG 
GAGCTTACCA GTCTGGTTGA GGCAGCGGCA GGTACCACGG AGGCAGGAGA TGATGATGGC 
ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG GAGCTGCTGG CCCCTGGGCC AGAGGACACA 
GAGCCCGCCC TAAGTGCAGA AGAACTGGCC GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC 
CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG 
GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA CAGGAGGAGG AGAAGGAGCA GATGGAGGAG 
GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG GGGGAGGACA GGCTGCACCT GCGGGCCTCG 
GTGAAGAGGA AAACCAGGCC GTGCTCCCTG CCTGTGTCCG AGCTAGAGAC GGTGATCGCG 
TCCGCTTGCG GTGAGCCGGA GACACCGCGG ACCCACTACA TCCGCATCCA CACGCTGCTG 
CACAGCCTGC CCTCGGCTCA GGGCGGCAGC GCGGACGACG ACGACGACGG TGCCGAGGAG 
GAGGCCACCC TCAAGGATGC CTCGGAGAAG GATGCGCTCA GTGAGATGGA CACGGTCGCC 
GCGGAGCCTC CTCCCCTGGA AGAGGATGGA GAGGCCCCCG AGGGGGCCAC TCCAGGCACG 
GCTCCCCTCG GCCACTCGGG CTTGGCTAAC GGCTCAGCCC CGGATGGCGA CGCGCTCCCC 
AGCACGGGGA GCGAGAGCGA CTCCAGCCCG CGGCAGGGTG GAGACCACAG CTGCTGCGAC 
GCATCCTGCT GCAGCCCCTC CTGCTACAGC TCCTCGTGCT ACAGCAGCTC CTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAGTAGGT TTGCCAGCCA CACCCGCTTC 
TCCTCTGTGG ACAGTGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA GGACGACGAG 
GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG CCCGACTCAG TGCAGAGCCC AGAGCTGGAC 
CAGGAGTCGG CCAACGGTGC AGGGCCATGG CAAGACGAGC TGGCCGCCCC CGATGGGAGC 
ATGGCCAGAA CCATGGAAGG TCTGGAGTCC CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CTTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAGTAAGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107131266 ( XM_015470322.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325808.1 Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa48883
Clone ID Related Accession (Same CDS sequence)	XM_015463084.1 , XM_015470322.1
Accession Version	XM_015470322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HECW1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TGTCAGAACC TAAAGAGGAC TGCCTTTCAG AGGAGATTTT TAAAAGCATT 
GGCTCTGATG AGACCGTGCA AGGGCAAGGA AGTCGGAGGC TGATCAGCTT TTCTCTCTCA 
GATTTCCAGG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTACCTGAAG 
ATTTCTATTC AGCCTGGGAA GCACAGCATC TTTCCTGCCC TCCCCCACCA TGGACAGGAG 
AGGCGATCCA AAATCATTGG CAACACTGTG AACCCCATCT GGCAGGCTGA GCAATTCAGC 
TTTGTGTCCT TGCCCACTGA TGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT TTTGGGAAAG CTGTCAATGC CCGTTCAAAG ACTCCTGGAA 
AGACATGCCA TAGGGGACAG GGTGGTCAGC TACACCCTTG GCCGTAGGCT TCCAACAGAT 
CACGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGATGAT 
GAGGAGATTT CCCTGAGTAC CGAGCCTGAG TCTGCGGAAA TTCAGGACAG CCCCATGAAT 
AACCTGGTGG CAAGCAGCTG TGGGGAGCCC CCGTGTGATG CACCAGAGCC CTCAGGGACC 
CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG 
GAGCTTACCA GTCTGGTTGA GGCAGCGGCA GGTACCACGG AGGCAGGAGA TGATGATGGC 
ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG GAGCTGCTGG CCCCTGGGCC AGAGGACACA 
GAGCCCGCCC TAAGTGCAGA AGAACTGGCC GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC 
CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG 
GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA CAGGAGGAGG AGAAGGAGCA GATGGAGGAG 
GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG GGGGAGGACA GGCTGCACCT GCGGGCCTCG 
GTGAAGAGGA AAACCAGGCC GTGCTCCCTG CCTGTGTCCG AGCTAGAGAC GGTGATCGCG 
TCCGCTTGCG GTGAGCCGGA GACACCGCGG ACCCACTACA TCCGCATCCA CACGCTGCTG 
CACAGCCTGC CCTCGGCTCA GGGCGGCAGC GCGGACGACG ACGACGACGG TGCCGAGGAG 
GAGGCCACCC TCAAGGATGC CTCGGAGAAG GATGCGCTCA GTGAGATGGA CACGGTCGCC 
GCGGAGCCTC CTCCCCTGGA AGAGGATGGA GAGGCCCCCG AGGGGGCCAC TCCAGGCACG 
GCTCCCCTCG GCCACTCGGG CTTGGCTAAC GGCTCAGCCC CGGATGGCGA CGCGCTCCCC 
AGCACGGGGA GCGAGAGCGA CTCCAGCCCG CGGCAGGGTG GAGACCACAG CTGCTGCGAC 
GCATCCTGCT GCAGCCCCTC CTGCTACAGC TCCTCGTGCT ACAGCAGCTC CTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAGTAGGT TTGCCAGCCA CACCCGCTTC 
TCCTCTGTGG ACAGTGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA GGACGACGAG 
GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG CCCGACTCAG TGCAGAGCCC AGAGCTGGAC 
CAGGAGTCGG CCAACGGTGC AGGGCCATGG CAAGACGAGC TGGCCGCCCC CGATGGGAGC 
ATGGCCAGAA CCATGGAAGG TCTGGAGTCC CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CTTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAGTAAGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325808.1
CDS	1597..3570
Translation	MAALSEPKEDCLSEEIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSPMNNLVASSCGEPPCDAPEPSGTPKPEQPSQGSTVGPPEIEGLELTSLVEAAAGTTEAGDDDGIVSVGPEGAGELLAPGPEDTEPALSAEELAERLDLGGEVALPGMLEDGQAPSSPGEPVAEERATESRAGGQEEEKEQMEEEEGDVSALGQGEDRLHLRASVKRKTRPCSLPVSELETVIASACGEPETPRTHYIRIHTLLHSLPSAQGGSADDDDDGAEEEATLKDASEKDALSEMDTVAAEPPPLEEDGEAPEGATPGTAPLGHSGLANGSAPDGDALPSTGSESDSSPRQGGDHSCCDASCCSPSCYSSSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDQESANGAGPWQDELAAPDGSMARTMEGLESPVAGPSSRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPSKL

Target ORF information:

RefSeq Version	XM_015470322.1
Organism	Bos taurus(cattle)
Definition	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470322.1

ORF Insert Sequence:

ATGGCTGCTC TGTCAGAACC TAAAGAGGAC TGCCTTTCAG AGGAGATTTT TAAAAGCATT 
GGCTCTGATG AGACCGTGCA AGGGCAAGGA AGTCGGAGGC TGATCAGCTT TTCTCTCTCA 
GATTTCCAGG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTACCTGAAG 
ATTTCTATTC AGCCTGGGAA GCACAGCATC TTTCCTGCCC TCCCCCACCA TGGACAGGAG 
AGGCGATCCA AAATCATTGG CAACACTGTG AACCCCATCT GGCAGGCTGA GCAATTCAGC 
TTTGTGTCCT TGCCCACTGA TGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT TTTGGGAAAG CTGTCAATGC CCGTTCAAAG ACTCCTGGAA 
AGACATGCCA TAGGGGACAG GGTGGTCAGC TACACCCTTG GCCGTAGGCT TCCAACAGAT 
CACGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGATGAT 
GAGGAGATTT CCCTGAGTAC CGAGCCTGAG TCTGCGGAAA TTCAGGACAG CCCCATGAAT 
AACCTGGTGG CAAGCAGCTG TGGGGAGCCC CCGTGTGATG CACCAGAGCC CTCAGGGACC 
CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG 
GAGCTTACCA GTCTGGTTGA GGCAGCGGCA GGTACCACGG AGGCAGGAGA TGATGATGGC 
ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG GAGCTGCTGG CCCCTGGGCC AGAGGACACA 
GAGCCCGCCC TAAGTGCAGA AGAACTGGCC GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC 
CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG 
GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA CAGGAGGAGG AGAAGGAGCA GATGGAGGAG 
GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG GGGGAGGACA GGCTGCACCT GCGGGCCTCG 
GTGAAGAGGA AAACCAGGCC GTGCTCCCTG CCTGTGTCCG AGCTAGAGAC GGTGATCGCG 
TCCGCTTGCG GTGAGCCGGA GACACCGCGG ACCCACTACA TCCGCATCCA CACGCTGCTG 
CACAGCCTGC CCTCGGCTCA GGGCGGCAGC GCGGACGACG ACGACGACGG TGCCGAGGAG 
GAGGCCACCC TCAAGGATGC CTCGGAGAAG GATGCGCTCA GTGAGATGGA CACGGTCGCC 
GCGGAGCCTC CTCCCCTGGA AGAGGATGGA GAGGCCCCCG AGGGGGCCAC TCCAGGCACG 
GCTCCCCTCG GCCACTCGGG CTTGGCTAAC GGCTCAGCCC CGGATGGCGA CGCGCTCCCC 
AGCACGGGGA GCGAGAGCGA CTCCAGCCCG CGGCAGGGTG GAGACCACAG CTGCTGCGAC 
GCATCCTGCT GCAGCCCCTC CTGCTACAGC TCCTCGTGCT ACAGCAGCTC CTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAGTAGGT TTGCCAGCCA CACCCGCTTC 
TCCTCTGTGG ACAGTGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA GGACGACGAG 
GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG CCCGACTCAG TGCAGAGCCC AGAGCTGGAC 
CAGGAGTCGG CCAACGGTGC AGGGCCATGG CAAGACGAGC TGGCCGCCCC CGATGGGAGC 
ATGGCCAGAA CCATGGAAGG TCTGGAGTCC CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CTTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAGTAAGCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107131266 ( XM_015463083.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318569.1 Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa89755
Clone ID Related Accession (Same CDS sequence)	XM_015463083.1 , XM_015470318.1
Accession Version	XM_015463083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HECW1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644732.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTCTT AGGCCTGGCC 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AATCGACGCC GGTGCAAGGA GCCGCTCCGC 
CACAGCTACA ATCCAGGCCA GTACCACAGT GTGGCCATCA GGACTGGCCC CCATGGTGCC 
GTCACCATCC CCCGCTCCAC CAGTGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACA 
CTCATGGTCA GCAGTTCCTA CTACTCCATA GGACACTCGC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGGGAC TGGATCGGCA TGTACCTCAT TGATGAGGTC 
TTGTCGGAAA ACTTCCTGGA CTATAAGAAC CGTGGAGTCA ATGGCTCTCA TCGGGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTAAG TGGAGCCTTG CGAGCCACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTGCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTTCCTGCCC TCCCCCACCA TGGACAGGAG AGGCGATCCA AAATCATTGG CAACACTGTG 
AACCCCATCT GGCAGGCTGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCAATGC CCGTTCAAAG ACTCCTGGAA AGACATGCCA TAGGGGACAG GGTGGTCAGC 
TACACCCTTG GCCGTAGGCT TCCAACAGAT CACGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCTGCGGAAA TTCAGGACAG CCCCATGAAT AACCTGGTGG CAAGCAGCTG TGGGGAGCCC 
CCGTGTGATG CACCAGAGCC CTCAGGGACC CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC 
ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG GAGCTTACCA GTCTGGTTGA GGCAGCGGCA 
GGTACCACGG AGGCAGGAGA TGATGATGGC ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG 
GAGCTGCTGG CCCCTGGGCC AGAGGACACA GAGCCCGCCC TAAGTGCAGA AGAACTGGCC 
GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC 
CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA 
CAGGAGGAGG AGAAGGAGCA GATGGAGGAG GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG 
GGGGAGGACA GGCTGCACCT GCGGGCCTCG GTGAAGAGGA AAACCAGGCC GTGCTCCCTG 
CCTGTGTCCG AGCTAGAGAC GGTGATCGCG TCCGCTTGCG GTGAGCCGGA GACACCGCGG 
ACCCACTACA TCCGCATCCA CACGCTGCTG CACAGCCTGC CCTCGGCTCA GGGCGGCAGC 
GCGGACGACG ACGACGACGG TGCCGAGGAG GAGGCCACCC TCAAGGATGC CTCGGAGAAG 
GATGCGCTCA GTGAGATGGA CACGGTCGCC GCGGAGCCTC CTCCCCTGGA AGAGGATGGA 
GAGGCCCCCG AGGGGGCCAC TCCAGGCACG GCTCCCCTCG GCCACTCGGG CTTGGCTAAC 
GGCTCAGCCC CGGATGGCGA CGCGCTCCCC AGCACGGGGA GCGAGAGCGA CTCCAGCCCG 
CGGCAGGGTG GAGACCACAG CTGCTGCGAC GCATCCTGCT GCAGCCCCTC CTGCTACAGC 
TCCTCGTGCT ACAGCAGCTC CTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAGTAGGT TTGCCAGCCA CACCCGCTTC TCCTCTGTGG ACAGTGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA GGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG 
CCCGACTCAG TGCAGAGCCC AGAGCTGGAC CAGGAGTCGG CCAACGGTGC AGGGCCATGG 
CAAGACGAGC TGGCCGCCCC CGATGGGAGC ATGGCCAGAA CCATGGAAGG TCTGGAGTCC 
CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318569.1
CDS	244..2619
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRHSYNPGQYHSVAIRTGPHGAVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSPMNNLVASSCGEPPCDAPEPSGTPKPEQPSQGSTVGPPEIEGLELTSLVEAAAGTTEAGDDDGIVSVGPEGAGELLAPGPEDTEPALSAEELAERLDLGGEVALPGMLEDGQAPSSPGEPVAEERATESRAGGQEEEKEQMEEEEGDVSALGQGEDRLHLRASVKRKTRPCSLPVSELETVIASACGEPETPRTHYIRIHTLLHSLPSAQGGSADDDDDGAEEEATLKDASEKDALSEMDTVAAEPPPLEEDGEAPEGATPGTAPLGHSGLANGSAPDGDALPSTGSESDSSPRQGGDHSCCDASCCSPSCYSSSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDQESANGAGPWQDELAAPDGSMARTMEGLESPVAGPSSRREG

Target ORF information:

RefSeq Version	XM_015463083.1
Organism	Bos taurus(cattle)
Definition	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463083.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTCTT AGGCCTGGCC 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AATCGACGCC GGTGCAAGGA GCCGCTCCGC 
CACAGCTACA ATCCAGGCCA GTACCACAGT GTGGCCATCA GGACTGGCCC CCATGGTGCC 
GTCACCATCC CCCGCTCCAC CAGTGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACA 
CTCATGGTCA GCAGTTCCTA CTACTCCATA GGACACTCGC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGGGAC TGGATCGGCA TGTACCTCAT TGATGAGGTC 
TTGTCGGAAA ACTTCCTGGA CTATAAGAAC CGTGGAGTCA ATGGCTCTCA TCGGGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTAAG TGGAGCCTTG CGAGCCACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTGCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTTCCTGCCC TCCCCCACCA TGGACAGGAG AGGCGATCCA AAATCATTGG CAACACTGTG 
AACCCCATCT GGCAGGCTGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCAATGC CCGTTCAAAG ACTCCTGGAA AGACATGCCA TAGGGGACAG GGTGGTCAGC 
TACACCCTTG GCCGTAGGCT TCCAACAGAT CACGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCTGCGGAAA TTCAGGACAG CCCCATGAAT AACCTGGTGG CAAGCAGCTG TGGGGAGCCC 
CCGTGTGATG CACCAGAGCC CTCAGGGACC CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC 
ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG GAGCTTACCA GTCTGGTTGA GGCAGCGGCA 
GGTACCACGG AGGCAGGAGA TGATGATGGC ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG 
GAGCTGCTGG CCCCTGGGCC AGAGGACACA GAGCCCGCCC TAAGTGCAGA AGAACTGGCC 
GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC 
CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA 
CAGGAGGAGG AGAAGGAGCA GATGGAGGAG GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG 
GGGGAGGACA GGCTGCACCT GCGGGCCTCG GTGAAGAGGA AAACCAGGCC GTGCTCCCTG 
CCTGTGTCCG AGCTAGAGAC GGTGATCGCG TCCGCTTGCG GTGAGCCGGA GACACCGCGG 
ACCCACTACA TCCGCATCCA CACGCTGCTG CACAGCCTGC CCTCGGCTCA GGGCGGCAGC 
GCGGACGACG ACGACGACGG TGCCGAGGAG GAGGCCACCC TCAAGGATGC CTCGGAGAAG 
GATGCGCTCA GTGAGATGGA CACGGTCGCC GCGGAGCCTC CTCCCCTGGA AGAGGATGGA 
GAGGCCCCCG AGGGGGCCAC TCCAGGCACG GCTCCCCTCG GCCACTCGGG CTTGGCTAAC 
GGCTCAGCCC CGGATGGCGA CGCGCTCCCC AGCACGGGGA GCGAGAGCGA CTCCAGCCCG 
CGGCAGGGTG GAGACCACAG CTGCTGCGAC GCATCCTGCT GCAGCCCCTC CTGCTACAGC 
TCCTCGTGCT ACAGCAGCTC CTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAGTAGGT TTGCCAGCCA CACCCGCTTC TCCTCTGTGG ACAGTGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA GGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG 
CCCGACTCAG TGCAGAGCCC AGAGCTGGAC CAGGAGTCGG CCAACGGTGC AGGGCCATGG 
CAAGACGAGC TGGCCGCCCC CGATGGGAGC ATGGCCAGAA CCATGGAAGG TCTGGAGTCC 
CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107131266 ( XM_015470318.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325804.1 Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X2, mRNA.

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CloneID	OBa89755
Clone ID Related Accession (Same CDS sequence)	XM_015463083.1 , XM_015470318.1
Accession Version	XM_015470318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase HECW1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103903.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTCTT AGGCCTGGCC 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AATCGACGCC GGTGCAAGGA GCCGCTCCGC 
CACAGCTACA ATCCAGGCCA GTACCACAGT GTGGCCATCA GGACTGGCCC CCATGGTGCC 
GTCACCATCC CCCGCTCCAC CAGTGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACA 
CTCATGGTCA GCAGTTCCTA CTACTCCATA GGACACTCGC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGGGAC TGGATCGGCA TGTACCTCAT TGATGAGGTC 
TTGTCGGAAA ACTTCCTGGA CTATAAGAAC CGTGGAGTCA ATGGCTCTCA TCGGGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTAAG TGGAGCCTTG CGAGCCACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTGCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTTCCTGCCC TCCCCCACCA TGGACAGGAG AGGCGATCCA AAATCATTGG CAACACTGTG 
AACCCCATCT GGCAGGCTGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCAATGC CCGTTCAAAG ACTCCTGGAA AGACATGCCA TAGGGGACAG GGTGGTCAGC 
TACACCCTTG GCCGTAGGCT TCCAACAGAT CACGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCTGCGGAAA TTCAGGACAG CCCCATGAAT AACCTGGTGG CAAGCAGCTG TGGGGAGCCC 
CCGTGTGATG CACCAGAGCC CTCAGGGACC CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC 
ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG GAGCTTACCA GTCTGGTTGA GGCAGCGGCA 
GGTACCACGG AGGCAGGAGA TGATGATGGC ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG 
GAGCTGCTGG CCCCTGGGCC AGAGGACACA GAGCCCGCCC TAAGTGCAGA AGAACTGGCC 
GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC 
CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA 
CAGGAGGAGG AGAAGGAGCA GATGGAGGAG GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG 
GGGGAGGACA GGCTGCACCT GCGGGCCTCG GTGAAGAGGA AAACCAGGCC GTGCTCCCTG 
CCTGTGTCCG AGCTAGAGAC GGTGATCGCG TCCGCTTGCG GTGAGCCGGA GACACCGCGG 
ACCCACTACA TCCGCATCCA CACGCTGCTG CACAGCCTGC CCTCGGCTCA GGGCGGCAGC 
GCGGACGACG ACGACGACGG TGCCGAGGAG GAGGCCACCC TCAAGGATGC CTCGGAGAAG 
GATGCGCTCA GTGAGATGGA CACGGTCGCC GCGGAGCCTC CTCCCCTGGA AGAGGATGGA 
GAGGCCCCCG AGGGGGCCAC TCCAGGCACG GCTCCCCTCG GCCACTCGGG CTTGGCTAAC 
GGCTCAGCCC CGGATGGCGA CGCGCTCCCC AGCACGGGGA GCGAGAGCGA CTCCAGCCCG 
CGGCAGGGTG GAGACCACAG CTGCTGCGAC GCATCCTGCT GCAGCCCCTC CTGCTACAGC 
TCCTCGTGCT ACAGCAGCTC CTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAGTAGGT TTGCCAGCCA CACCCGCTTC TCCTCTGTGG ACAGTGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA GGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG 
CCCGACTCAG TGCAGAGCCC AGAGCTGGAC CAGGAGTCGG CCAACGGTGC AGGGCCATGG 
CAAGACGAGC TGGCCGCCCC CGATGGGAGC ATGGCCAGAA CCATGGAAGG TCTGGAGTCC 
CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325804.1
CDS	244..2619
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRHSYNPGQYHSVAIRTGPHGAVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAEIQDSPMNNLVASSCGEPPCDAPEPSGTPKPEQPSQGSTVGPPEIEGLELTSLVEAAAGTTEAGDDDGIVSVGPEGAGELLAPGPEDTEPALSAEELAERLDLGGEVALPGMLEDGQAPSSPGEPVAEERATESRAGGQEEEKEQMEEEEGDVSALGQGEDRLHLRASVKRKTRPCSLPVSELETVIASACGEPETPRTHYIRIHTLLHSLPSAQGGSADDDDDGAEEEATLKDASEKDALSEMDTVAAEPPPLEEDGEAPEGATPGTAPLGHSGLANGSAPDGDALPSTGSESDSSPRQGGDHSCCDASCCSPSCYSSSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDQESANGAGPWQDELAAPDGSMARTMEGLESPVAGPSSRREG

Target ORF information:

RefSeq Version	XM_015470318.1
Organism	Bos taurus(cattle)
Definition	Bos taurus E3 ubiquitin-protein ligase HECW1-like (LOC107131266), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470318.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTCTT AGGCCTGGCC 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AATCGACGCC GGTGCAAGGA GCCGCTCCGC 
CACAGCTACA ATCCAGGCCA GTACCACAGT GTGGCCATCA GGACTGGCCC CCATGGTGCC 
GTCACCATCC CCCGCTCCAC CAGTGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACA 
CTCATGGTCA GCAGTTCCTA CTACTCCATA GGACACTCGC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGGGAC TGGATCGGCA TGTACCTCAT TGATGAGGTC 
TTGTCGGAAA ACTTCCTGGA CTATAAGAAC CGTGGAGTCA ATGGCTCTCA TCGGGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTAGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTAAG TGGAGCCTTG CGAGCCACCA CCCCCAGTGT CACCGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTGCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCTATTC AGCCTGGGAA GCACAGCATC 
TTTCCTGCCC TCCCCCACCA TGGACAGGAG AGGCGATCCA AAATCATTGG CAACACTGTG 
AACCCCATCT GGCAGGCTGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT TTTGGGAAAG 
CTGTCAATGC CCGTTCAAAG ACTCCTGGAA AGACATGCCA TAGGGGACAG GGTGGTCAGC 
TACACCCTTG GCCGTAGGCT TCCAACAGAT CACGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCTGCGGAAA TTCAGGACAG CCCCATGAAT AACCTGGTGG CAAGCAGCTG TGGGGAGCCC 
CCGTGTGATG CACCAGAGCC CTCAGGGACC CCAAAGCCTG AGCAGCCGAG CCAGGGCAGC 
ACTGTGGGCC CTCCAGAGAT CGAGGGCCTG GAGCTTACCA GTCTGGTTGA GGCAGCGGCA 
GGTACCACGG AGGCAGGAGA TGATGATGGC ATAGTCTCTG TGGGGCCTGA GGGGGCAGGG 
GAGCTGCTGG CCCCTGGGCC AGAGGACACA GAGCCCGCCC TAAGTGCAGA AGAACTGGCC 
GAGCGGCTGG ACCTGGGAGG GGAGGTGGCC CTCCCGGGGA TGCTGGAGGA CGGTCAAGCC 
CCTAGCAGCC CTGGGGAGCC GGTGGCTGAG GAAAGGGCCA CAGAGAGTCG GGCTGGGGGA 
CAGGAGGAGG AGAAGGAGCA GATGGAGGAG GAAGAGGGGG ACGTGTCTGC CCTGGGGCAG 
GGGGAGGACA GGCTGCACCT GCGGGCCTCG GTGAAGAGGA AAACCAGGCC GTGCTCCCTG 
CCTGTGTCCG AGCTAGAGAC GGTGATCGCG TCCGCTTGCG GTGAGCCGGA GACACCGCGG 
ACCCACTACA TCCGCATCCA CACGCTGCTG CACAGCCTGC CCTCGGCTCA GGGCGGCAGC 
GCGGACGACG ACGACGACGG TGCCGAGGAG GAGGCCACCC TCAAGGATGC CTCGGAGAAG 
GATGCGCTCA GTGAGATGGA CACGGTCGCC GCGGAGCCTC CTCCCCTGGA AGAGGATGGA 
GAGGCCCCCG AGGGGGCCAC TCCAGGCACG GCTCCCCTCG GCCACTCGGG CTTGGCTAAC 
GGCTCAGCCC CGGATGGCGA CGCGCTCCCC AGCACGGGGA GCGAGAGCGA CTCCAGCCCG 
CGGCAGGGTG GAGACCACAG CTGCTGCGAC GCATCCTGCT GCAGCCCCTC CTGCTACAGC 
TCCTCGTGCT ACAGCAGCTC CTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAGTAGGT TTGCCAGCCA CACCCGCTTC TCCTCTGTGG ACAGTGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA GGACGACGAG GAGGAGGAGA ACAGCGCCTT CGAGTCAGTG 
CCCGACTCAG TGCAGAGCCC AGAGCTGGAC CAGGAGTCGG CCAACGGTGC AGGGCCATGG 
CAAGACGAGC TGGCCGCCCC CGATGGGAGC ATGGCCAGAA CCATGGAAGG TCTGGAGTCC 
CCCGTGGCAG GGCCGAGCAG TCGGAGAGAA GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC107131266 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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