LOC104974671 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104974671
Entrez Gene ID	104974671
Full Name	phospholipase A2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015467117.1	XP_015322603.1	phospholipase A2
XM_010814065.2	XP_010812367.1	phospholipase A2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Differential participation of phospholipase A2 isoforms during iron-induced retinal toxicity. Implications for age-related macular degeneration.
Rodr?guez Diez G, Uranga RM, Mateos MV, Giusto NM, Salvador GA
Neurochemistry international61(5)749-58(2012 Oct)

Surfactant protein B inhibits secretory phospholipase A2 hydrolysis of surfactant phospholipids.
Hite RD, Grier BL, Waite BM, Veldhuizen RA, Possmayer F, Yao LJ, Seeds MC
American journal of physiology. Lung cellular and molecular physiology302(2)L257-65(2012 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Suggestive evidence for the involvement of the second calcium and surface loop in interfacial binding: monoclinic and trigonal crystal structures of a quadruple mutant of phospholipase A2.
Sekar K, Yogavel M, Kanaujia SP, Sharma A, Velmurugan D, Poi MJ, Dauter Z, Tsai MD
Acta crystallographica. Section D, Biological crystallography62(Pt 7)717-24(2006 Jul)

Third calcium ion found in an inhibitor-bound phospholipase A2.
Sekar K, Gayathri D, Velmurugan D, Jeyakanthan J, Yamane T, Poi MJ, Tsai MD
Acta crystallographica. Section D, Biological crystallography62(Pt 4)392-7(2006 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC104974671 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104974671 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44252	XM_015467117.1 Latest version!	Bos taurus phospholipase A2 (LOC104974671), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa44252	XM_010814065.2 Latest version!	Bos taurus phospholipase A2 (LOC104974671), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104974671 ( XM_015467117.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322603.1 Bos taurus phospholipase A2 (LOC104974671), mRNA.

Price & Availability↑

CloneID	OBa44252
Clone ID Related Accession (Same CDS sequence)	XM_015467117.1 , XM_010814065.2
Accession Version	XM_015467117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phospholipase A2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) and transcript sequence (NM_174646.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 342 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGACTCC TGGTGTTGGC TGCTCTGCTC ACAGTGGGCG CTGGCCAGGC CGGCCTCAAC 
TCGAGGGCAT TATGGCAGTT CAACGGCATG ATTAAGTGCA AGATCCCCAG CAGTGAACCA 
TTGCTGGATT TCAACAACTA TGGCTGCTAT TGTGGCCTGG GTGGATCAGG GACCCCTGTG 
GATGATCTGG ACAGGTGCTG CCAGACGCAC GACAACTGCT ACAAACAAGC CAAGAAACTG 
GACAGCTGTA AAGTCCTCGT GGACAATCCC TACACCAACA ACTACTCCTA CTCATGTTCT 
AACAATGAGA TCACCTGCAG CAGCGAAAAC AATGCCTGTG AGGCCTTCAT CTGCAACTGT 
GACCGCAATG CCGCCATCTG CTTCTCAAAG GTGCCATACA ACAAGGAGCA CAAGAACCTG 
GACAAGAAGA ACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322603.1
CDS	88..525
Translation	MRLLVLAALLTVGAGQAGLNSRALWQFNGMIKCKIPSSEPLLDFNNYGCYCGLGGSGTPVDDLDRCCQTHDNCYKQAKKLDSCKVLVDNPYTNNYSYSCSNNEITCSSENNACEAFICNCDRNAAICFSKVPYNKEHKNLDKKNC

Target ORF information:

RefSeq Version	XM_015467117.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase A2 (LOC104974671), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015467117.1

ORF Insert Sequence:

ATGAGACTCC TGGTGTTGGC TGCTCTGCTC ACAGTGGGCG CTGGCCAGGC CGGCCTCAAC 
TCGAGGGCAT TATGGCAGTT CAACGGCATG ATTAAGTGCA AGATCCCCAG CAGTGAACCA 
TTGCTGGATT TCAACAACTA TGGCTGCTAT TGTGGCCTGG GTGGATCAGG GACCCCTGTG 
GATGATCTGG ACAGGTGCTG CCAGACGCAC GACAACTGCT ACAAACAAGC CAAGAAACTG 
GACAGCTGTA AAGTCCTCGT GGACAATCCC TACACCAACA ACTACTCCTA CTCATGTTCT 
AACAATGAGA TCACCTGCAG CAGCGAAAAC AATGCCTGTG AGGCCTTCAT CTGCAACTGT 
GACCGCAATG CCGCCATCTG CTTCTCAAAG GTGCCATACA ACAAGGAGCA CAAGAACCTG 
GACAAGAAGA ACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104974671 ( XM_010814065.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812367.1 Bos taurus phospholipase A2 (LOC104974671), mRNA.

Price & Availability↑

CloneID	OBa44252
Clone ID Related Accession (Same CDS sequence)	XM_015467117.1 , XM_010814065.2
Accession Version	XM_010814065.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phospholipase A2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) and transcript sequence (NM_174646.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814065.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 342 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGACTCC TGGTGTTGGC TGCTCTGCTC ACAGTGGGCG CTGGCCAGGC CGGCCTCAAC 
TCGAGGGCAT TATGGCAGTT CAACGGCATG ATTAAGTGCA AGATCCCCAG CAGTGAACCA 
TTGCTGGATT TCAACAACTA TGGCTGCTAT TGTGGCCTGG GTGGATCAGG GACCCCTGTG 
GATGATCTGG ACAGGTGCTG CCAGACGCAC GACAACTGCT ACAAACAAGC CAAGAAACTG 
GACAGCTGTA AAGTCCTCGT GGACAATCCC TACACCAACA ACTACTCCTA CTCATGTTCT 
AACAATGAGA TCACCTGCAG CAGCGAAAAC AATGCCTGTG AGGCCTTCAT CTGCAACTGT 
GACCGCAATG CCGCCATCTG CTTCTCAAAG GTGCCATACA ACAAGGAGCA CAAGAACCTG 
GACAAGAAGA ACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812367.1
CDS	88..525
Translation	MRLLVLAALLTVGAGQAGLNSRALWQFNGMIKCKIPSSEPLLDFNNYGCYCGLGGSGTPVDDLDRCCQTHDNCYKQAKKLDSCKVLVDNPYTNNYSYSCSNNEITCSSENNACEAFICNCDRNAAICFSKVPYNKEHKNLDKKNC

Target ORF information:

RefSeq Version	XM_010814065.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase A2 (LOC104974671), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814065.2

ORF Insert Sequence:

ATGAGACTCC TGGTGTTGGC TGCTCTGCTC ACAGTGGGCG CTGGCCAGGC CGGCCTCAAC 
TCGAGGGCAT TATGGCAGTT CAACGGCATG ATTAAGTGCA AGATCCCCAG CAGTGAACCA 
TTGCTGGATT TCAACAACTA TGGCTGCTAT TGTGGCCTGG GTGGATCAGG GACCCCTGTG 
GATGATCTGG ACAGGTGCTG CCAGACGCAC GACAACTGCT ACAAACAAGC CAAGAAACTG 
GACAGCTGTA AAGTCCTCGT GGACAATCCC TACACCAACA ACTACTCCTA CTCATGTTCT 
AACAATGAGA TCACCTGCAG CAGCGAAAAC AATGCCTGTG AGGCCTTCAT CTGCAACTGT 
GACCGCAATG CCGCCATCTG CTTCTCAAAG GTGCCATACA ACAAGGAGCA CAAGAACCTG 
GACAAGAAGA ACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential participation of phospholipase A2 isoforms during iron-induced retinal toxicity. Implications for age-related macular degeneration.
Neurochemistry international61(5)749-58(2012 Oct)
Rodr?guez Diez G,Uranga RM,Mateos MV,Giusto NM,Salvador GA

Surfactant protein B inhibits secretory phospholipase A2 hydrolysis of surfactant phospholipids.
American journal of physiology. Lung cellular and molecular physiology302(2)L257-65(2012 Jan)
Hite RD,Grier BL,Waite BM,Veldhuizen RA,Possmayer F,Yao LJ,Seeds MC

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Suggestive evidence for the involvement of the second calcium and surface loop in interfacial binding: monoclinic and trigonal crystal structures of a quadruple mutant of phospholipase A2.
Acta crystallographica. Section D, Biological crystallography62(Pt 7)717-24(2006 Jul)
Sekar K,Yogavel M,Kanaujia SP,Sharma A,Velmurugan D,Poi MJ,Dauter Z,Tsai MD

Third calcium ion found in an inhibitor-bound phospholipase A2.
Acta crystallographica. Section D, Biological crystallography62(Pt 4)392-7(2006 Apr)
Sekar K,Gayathri D,Velmurugan D,Jeyakanthan J,Yamane T,Poi MJ,Tsai MD

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LOC104974671 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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