COL5A1 cDNA ORF clone, Bos taurus(cattle)

The following COL5A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221060 XM_024999726.1
Latest version!
Bos taurus collagen type V alpha 1 chain (COL5A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa221061 XM_024999727.1
Latest version!
Bos taurus collagen type V alpha 1 chain (COL5A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa92066 XM_015465612.1
Latest version!
Bos taurus collagen type V alpha 1 (COL5A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa92066 XM_015473738.1
Latest version!
Bos taurus collagen type V alpha 1 (COL5A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa221060
    Clone ID Related Accession (Same CDS sequence) XM_024999726.1
    Accession Version XM_024999726.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-1(V) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGACGTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
    TCCCCGCCGC CGCTGCTGCT GCTGCTGCTG CTGCTGCTGT GGGCGCCGCC TCCGAGCCGC
    GCAGCTCAGC CAGCAGATCT CCTGAAGGTT CTAGATTTTC ACAACCTGCC TGATGGAATA
    ACCAAGACCA CAGGCTTCTG TGCAGCGCGG CGATCTTCCA AAGGCCCGGA TGTCGCCTAC
    AGAGTCACGA AAGATGCCCA GCTCAGCGCG CCCACCAAGC AGCTGTACCC TGCATCTGCG
    TTTCCTGAGG ACTTCTCCAT CCTAACCACT GTGAAAGCCA AGAAAGGCAG CCAGGCCTTC
    CTGGTCTCCA TCTACAATGA GCAGGGCATC CAGCAGATCG GCCTGGAGAT GGGCCGCTCT
    CCGGTCTTCC TCTATGAGGA CCACACGGGG AAGCCGGGGC CAGAGGACTA CCCCCTCTTC
    AGAGGCATCA ACCTGTCAGA TGGCAAGTGG CACAGAATTG CTCTCAGCGT CCACAAGAAG
    AATGTCACCT TGATCCTGGA CTGTAAAAAG AAGACAACCA AGTTCCTGGA CCGCAGTGAA
    CACCCTGTGA TAGACGTCAA CGGCATCGTC GTGTTCGGCA CCCGGATTCT GGATGAGGAA
    GTGTTTGAGG GTGACATCCA GCAGCTGCTC CTCGTCTCGG ACCACCGGGC GGCTTACGAT
    TACTGCGAGC ACTACAGCCC CGACTGTGAC ACGGCCGTCC CCGACAAACC CCAGTCTCAG
    GACCCCAACC CAGATGAATA CTACCCAGAC GGAGACAACG AAGGAGATAC CTATTACTAC
    GAGTACCCCT ACTATGAGGA CACGGACGAC ACAGGCAAAG ACCCCATCAC CACCAAGACG
    CCCGTGGAGG CTGCTCGAGA GACCACGGAG GTGGCTGAGG AGCTGACCCA GCCCCCGACG
    GAAGCCCCTT CGGCGCCCAG CACCACCGAG GGCCCCGGGA AGGAGGAGGA CCCCGGCATC
    GGCGACTACG ACTACGTGCC CAGTGAAGAC TACTACACGC CCCCGCCTTA CGAGGACTTT
    GGCTACGGCG AAGGCATTGA GAACCCGGAT GAGAACCCCG ACCAGCTCCC GGACCAGGGC
    GCCCGGGCCG AGGTTCCCAC CAGCACGGTC CTCACCTCCA ACGACTCCAA CCCGGCCCCG
    CCTCCGGAGG AGGGGATAGA TGACCTGGAG GAAGAGTTCA CGGAAGAAAC CATCAAGAAC
    CTGGACGAGA GCTACTATGA CCCGTACTAC GACCCCACCA TCTCCCCATC GGAGATCGGG
    CCAGGCATGC CTGCCAACCA GGACACCATC TATGAGGGGA TGGGAGGGCC TCGGGGCGAG
    AAAGGTCAAA AAGGAGAACC CGCCATCATC GAACCGGGGA TGCTCCTGGA GGGGCCGCCT
    GGCCCCGAAG GCCCCGCGGG TCTCCCAGGA CCTCCAGGAA CGATGGGTCC CACAGGCCAA
    GTGGGTGACC CTGGAGAAAG AGGTCCCCCT GGACGCCCAG GCCTTCCTGG TGCTGACGGG
    CTGCCCGGCC CTCCTGGAAC CATGCTTATG CTGCCTTTCC GGTTCGGAGG GGGCGGCGAC
    GCGGGCTCCA AAGGACCCAT GGTCTCCGCG CAGGAGTCCC AAGCTCAAGC CATCCTGCAA
    CAGGCCCGGC TGGCGTTGAG GGGGCCTGCT GGCCCAATGG GTCTCACTGG GAGACCGGGT
    CCCATGGGCC CTCCTGGGAG CGGAGGTCTG AAGGGTGAGC CAGGAGACAT GGGGCCTCAG
    GGTCCGCGGG GTGTGCAGGG CCCGCCTGGC CCGGCAGGAA AGCCTGGGAG AAGGGGCCGA
    GCCGGCAGTG ATGGAGCGAG AGGGATGCCT GGGCAGACTG GCCCCAAGGG TGACCGCGGC
    TTCGATGGCC TGGCGGGGCT GCCGGGGGAG AAGGGCCACC GGGGCGACCC TGGCCCGTCC
    GGCCCACCGG GACCTCCGGG GGAAGACGGA GAAAGGGGCG ACGATGGGGA AGTCGGGCCC
    AGAGGGCTGC CTGGGGAGCC CGGGCCGCGA GGTCTGCTGG GGCCGAAGGG GCCCCCAGGC
    CCCCCTGGAC CGCCCGGTGT GACGGGAATG GATGGTCAAC CAGGCCCAAA AGGCAACGTG
    GGTCCCCAGG GAGAGCCCGG CCCCCCGGGG CAGCAGGGCA ACCCAGGTGC CCAGGGTCTT
    CCGGGCCCCC AGGGCGCTAT CGGGCCTCCA GGAGAAAAGG GTCCCCTGGG CAAACCCGGC
    CTCCCAGGAA TGCCTGGTGC TGACGGACCC CCGGGGCACC CTGGCAAAGA AGGCCCTCCA
    GGAGAGAAGG GAGGGCAGGG TCCTCCCGGC CCCCAGGGTC CAATCGGCTA CCCAGGTCCT
    CGCGGAGTCA AGGGGGCAGA CGGCATCCGA GGTCTGAAGG GCACCAAGGG TGAGAAGGGT
    GAAGATGGCT TCCCAGGCTT CAAAGGAGAC ATGGGCATCA AGGGCGATCG GGGGGAGATC
    GGCCCACCTG GTCCCAGAGG GGAGGACGGT CCTGAAGGTC CCAAGGGGCG AGGCGGTCCG
    AACGGCGACC CCGGTCCTCT GGGACCCCCT GGGGAGAAGG GCAAACTCGG AGTCCCAGGA
    CTGCCAGGCT ACCCAGGAAG ACAGGGGCCA AAGGGTTCTA TCGGATTTCC TGGATTTCCC
    GGCGCCAACG GAGAAAAGGG CGGCAGGGGC ACCCCTGGGA AGCCGGGACC ACGGGGGCAG
    CGAGGCCCGA CGGGCCCACG AGGTGAAAGA GGCCCACGGG GCATCACCGG CAAGCCTGGC
    CCCAAGGGCA ACTCTGGAGG CGATGGCCCG GCCGGCCCTC CAGGTGAACG GGGACCCAAC
    GGACCCCAAG GACCCACGGG GTTCCCTGGA CCAAAGGGCC CCCCGGGGCC CCCAGGCAAG
    GACGGGCTCC CGGGGCACCC TGGACAGCGA GGCGAGACGG GTTTCCAAGG CAAGACCGGC
    CCTCCAGGCC CCCCAGGTGT GGTCGGCCCT CAGGGTCCCA CCGGGGAAAC TGGTCCCATG
    GGCGAGCGTG GCCACCCTGG GCCCCCAGGC CCCCCCGGTG AACAGGGGCT CCCGGGCCTG
    GCCGGGAAAG AAGGGACGAA GGGTGACCCA GGCCCCGCCG GCCTTCCTGG GAAAGACGGT
    CCCCCCGGGC TGCGAGGCTT CCCTGGAGAC CGAGGCCTTC CTGGTCCAGT GGGGGCGCTT
    GGACTGAAAG GCAGTGAAGG CCCCCCCGGC CCACCAGGCC CCGCGGGATC TCCAGGCGAG
    AGAGGTCCAG CCGGTGCTGC TGGGCCCATT GGAATTCCAG GGAGACCCGG GCCCCAGGGG
    CCCCCTGGGC CAGCTGGAGA GAAAGGCGCT CCTGGCGAGA AGGGTCCACA AGGCCCAGCT
    GGCCGAGACG GTCTCCAGGG CCCCGTGGGG CTCCCCGGTC CCGCTGGCCC TGTGGGCCCC
    CCTGGAGAAG ATGGAGACAA GGGAGAGATT GGGGAGCCAG GACAGAAAGG GAGCAAGGGA
    GACAAAGGCG AGCAGGGTCC ACCAGGGCCT ACAGGACCTC AAGGCCCCAT CGGACAGCCA
    GGCCCCTCCG GAGCCGATGG CGAGCCGGGA CCTCGGGGCC AGCAGGGCCT CTTTGGGCAG
    AAAGGCGACG AGGGTCCGAG AGGCTTCCCC GGGCCCCCGG GCCCCGTGGG GCTGCAGGGT
    CTGCCGGGAC CTCCCGGAGA GAAGGGCGAG ACAGGCGACG TGGGTCAGAT GGGCCCGCCA
    GGTCCCCCCG GACCCCGAGG ACCCTCCGGA GCTCCAGGTG CAGACGGGCC ACAAGGACCC
    CCCGGCGGAA TAGGCAACCC TGGAGCCGTG GGAGAGAAGG GTGAACCCGG CGAAGCAGGA
    GAGCCTGGCC TTCCCGGCGA AGGGGGCCCC CCGGGACCCA AAGGCGAAAG GGGGGAGAAA
    GGCGAGTCGG GCCCCTCCGG TGCCGCCGGA CCCCCGGGGC CCAAGGGCCC TCCCGGAGAC
    GACGGTCCCA AAGGCAGCCC CGGCCCCGTC GGTTTTCCCG GAGATCCCGG TCCCCCCGGA
    GAACCCGGCC CCGCGGGTCA AGACGGTCCC CCTGGCGACA AAGGAGACGA TGGTGAACCC
    GGGCAAACGG GATCACCAGG CCCTACGGGT GAGCCAGGCC CATCTGGGCC TCCAGGAAAA
    AGGGGTCCGC CCGGCCCCGC AGGTCCCGAA GGCAGGCAGG GGGAGAAAGG AGCCAAGGGA
    GAAGCCGGCT TGGAAGGCCC TCCTGGGAAG ACTGGCCCCA TTGGCCCCCA GGGGGCCCCC
    GGGAAGCCGG GGCCCGACGG CCTTCGAGGG ATCCCGGGCC CAGTGGGTGA ACAAGGTCTC
    CCCGGAGCCC CGGGCCCTGA TGGCCCCCCA GGACCCATGG GCCCCCCAGG ACTCCCCGGC
    CTCAAAGGAG ACTCTGGACC CAAAGGAGAG AAGGGTCACC CAGGCTTGAT CGGCCTCATC
    GGACCTCCGG GCGAACAGGG AGAAAAGGGT GACCGCGGAG TCCCCGGCCC CCAGGGCTCC
    TCGGGCCCTA AGGGAGAACA GGGTATCACC GGTCCTTCCG GCCCGATTGG CCCCCCGGGT
    CCCCCCGGCC TGCCGGGGCC CCCTGGTCCA AAAGGCGCTA AGGGCTCCTC GGGTCCCACT
    GGCCCGAAGG GTGAGGCAGG CCAGCCAGGA CCCCCTGGCC CACCAGGCCC CCCAGGCGAG
    GTCATCCAGC CCCTGCCGAT CCAGGCATCC AGGACCCGGC GGAATATCGA TGCCAGCCAG
    CTGATGGACG ACGCAGAGGG CGAGCAGTAC ATGGACTATG CGGATGGCAT GGAGGAGATC
    TTCGGCTCGC TCAACTCCCT GAAGCTGGAG ATCGAGCAGA TGAAGCGGCC GCTGGGCACA
    CCGCAGAACC CCGCACGCAC CTGCAAAGAC CTCCAGCTCT GCCACCCCGA CTTCCCTGAC
    GGCGAATACT GGGTGGATCC GAACCAAGGC TGCTCCCGGG ACTCCTTCAA GGTCTACTGC
    AACTTCACGG CCGGGGGGTC AACGTGCGTC TTCCCCGACA AGAAGTCTGA AGGGGCCAGA
    ATCACTTCTT GGCCCAAAGA AAACCCGGGT TCCTGGTTCA GTGAATTCAA GCGTGGCAAA
    CTGCTCTCCT ATGTGGACGC CGAGGGCAAC CCCGTGGGCG TCGTCCAGAT GACCTTCCTG
    CGGCTGCTCA GCGCCTCCGC CCACCAGAAC ATCACCTACA ACTGCTACCA GTCGGTGGCC
    TGGCAGGACG CCGCCACGGG CAGCTATGAC AAGGCCATGC GCTTCCTGGG CTCCAACGAC
    GAGGAGATGT CATATGACAA CAGCCCCTAC ATCCGCGCCC TGGTGGACGG CTGCGCCACG
    AAGAAAGGGT ATCAGAAGAC GGTTCTGGAG ATCGACACGC CCAAGGTGGA GCAGGTGCCC
    ATCGTCGACA TCATGTTCAA CGACTTCGGT GAAGCGTCAC AGAAATTTGG ATTTGAAGTG
    GGGCCGGCTT GCTTCCTGGG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855494.1
    CDS409..5952
    Translation

    Target ORF information:

    RefSeq Version XM_024999726.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 1 chain (COL5A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGACGTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
    TCCCCGCCGC CGCTGCTGCT GCTGCTGCTG CTGCTGCTGT GGGCGCCGCC TCCGAGCCGC
    GCAGCTCAGC CAGCAGATCT CCTGAAGGTT CTAGATTTTC ACAACCTGCC TGATGGAATA
    ACCAAGACCA CAGGCTTCTG TGCAGCGCGG CGATCTTCCA AAGGCCCGGA TGTCGCCTAC
    AGAGTCACGA AAGATGCCCA GCTCAGCGCG CCCACCAAGC AGCTGTACCC TGCATCTGCG
    TTTCCTGAGG ACTTCTCCAT CCTAACCACT GTGAAAGCCA AGAAAGGCAG CCAGGCCTTC
    CTGGTCTCCA TCTACAATGA GCAGGGCATC CAGCAGATCG GCCTGGAGAT GGGCCGCTCT
    CCGGTCTTCC TCTATGAGGA CCACACGGGG AAGCCGGGGC CAGAGGACTA CCCCCTCTTC
    AGAGGCATCA ACCTGTCAGA TGGCAAGTGG CACAGAATTG CTCTCAGCGT CCACAAGAAG
    AATGTCACCT TGATCCTGGA CTGTAAAAAG AAGACAACCA AGTTCCTGGA CCGCAGTGAA
    CACCCTGTGA TAGACGTCAA CGGCATCGTC GTGTTCGGCA CCCGGATTCT GGATGAGGAA
    GTGTTTGAGG GTGACATCCA GCAGCTGCTC CTCGTCTCGG ACCACCGGGC GGCTTACGAT
    TACTGCGAGC ACTACAGCCC CGACTGTGAC ACGGCCGTCC CCGACAAACC CCAGTCTCAG
    GACCCCAACC CAGATGAATA CTACCCAGAC GGAGACAACG AAGGAGATAC CTATTACTAC
    GAGTACCCCT ACTATGAGGA CACGGACGAC ACAGGCAAAG ACCCCATCAC CACCAAGACG
    CCCGTGGAGG CTGCTCGAGA GACCACGGAG GTGGCTGAGG AGCTGACCCA GCCCCCGACG
    GAAGCCCCTT CGGCGCCCAG CACCACCGAG GGCCCCGGGA AGGAGGAGGA CCCCGGCATC
    GGCGACTACG ACTACGTGCC CAGTGAAGAC TACTACACGC CCCCGCCTTA CGAGGACTTT
    GGCTACGGCG AAGGCATTGA GAACCCGGAT GAGAACCCCG ACCAGCTCCC GGACCAGGGC
    GCCCGGGCCG AGGTTCCCAC CAGCACGGTC CTCACCTCCA ACGACTCCAA CCCGGCCCCG
    CCTCCGGAGG AGGGGATAGA TGACCTGGAG GAAGAGTTCA CGGAAGAAAC CATCAAGAAC
    CTGGACGAGA GCTACTATGA CCCGTACTAC GACCCCACCA TCTCCCCATC GGAGATCGGG
    CCAGGCATGC CTGCCAACCA GGACACCATC TATGAGGGGA TGGGAGGGCC TCGGGGCGAG
    AAAGGTCAAA AAGGAGAACC CGCCATCATC GAACCGGGGA TGCTCCTGGA GGGGCCGCCT
    GGCCCCGAAG GCCCCGCGGG TCTCCCAGGA CCTCCAGGAA CGATGGGTCC CACAGGCCAA
    GTGGGTGACC CTGGAGAAAG AGGTCCCCCT GGACGCCCAG GCCTTCCTGG TGCTGACGGG
    CTGCCCGGCC CTCCTGGAAC CATGCTTATG CTGCCTTTCC GGTTCGGAGG GGGCGGCGAC
    GCGGGCTCCA AAGGACCCAT GGTCTCCGCG CAGGAGTCCC AAGCTCAAGC CATCCTGCAA
    CAGGCCCGGC TGGCGTTGAG GGGGCCTGCT GGCCCAATGG GTCTCACTGG GAGACCGGGT
    CCCATGGGCC CTCCTGGGAG CGGAGGTCTG AAGGGTGAGC CAGGAGACAT GGGGCCTCAG
    GGTCCGCGGG GTGTGCAGGG CCCGCCTGGC CCGGCAGGAA AGCCTGGGAG AAGGGGCCGA
    GCCGGCAGTG ATGGAGCGAG AGGGATGCCT GGGCAGACTG GCCCCAAGGG TGACCGCGGC
    TTCGATGGCC TGGCGGGGCT GCCGGGGGAG AAGGGCCACC GGGGCGACCC TGGCCCGTCC
    GGCCCACCGG GACCTCCGGG GGAAGACGGA GAAAGGGGCG ACGATGGGGA AGTCGGGCCC
    AGAGGGCTGC CTGGGGAGCC CGGGCCGCGA GGTCTGCTGG GGCCGAAGGG GCCCCCAGGC
    CCCCCTGGAC CGCCCGGTGT GACGGGAATG GATGGTCAAC CAGGCCCAAA AGGCAACGTG
    GGTCCCCAGG GAGAGCCCGG CCCCCCGGGG CAGCAGGGCA ACCCAGGTGC CCAGGGTCTT
    CCGGGCCCCC AGGGCGCTAT CGGGCCTCCA GGAGAAAAGG GTCCCCTGGG CAAACCCGGC
    CTCCCAGGAA TGCCTGGTGC TGACGGACCC CCGGGGCACC CTGGCAAAGA AGGCCCTCCA
    GGAGAGAAGG GAGGGCAGGG TCCTCCCGGC CCCCAGGGTC CAATCGGCTA CCCAGGTCCT
    CGCGGAGTCA AGGGGGCAGA CGGCATCCGA GGTCTGAAGG GCACCAAGGG TGAGAAGGGT
    GAAGATGGCT TCCCAGGCTT CAAAGGAGAC ATGGGCATCA AGGGCGATCG GGGGGAGATC
    GGCCCACCTG GTCCCAGAGG GGAGGACGGT CCTGAAGGTC CCAAGGGGCG AGGCGGTCCG
    AACGGCGACC CCGGTCCTCT GGGACCCCCT GGGGAGAAGG GCAAACTCGG AGTCCCAGGA
    CTGCCAGGCT ACCCAGGAAG ACAGGGGCCA AAGGGTTCTA TCGGATTTCC TGGATTTCCC
    GGCGCCAACG GAGAAAAGGG CGGCAGGGGC ACCCCTGGGA AGCCGGGACC ACGGGGGCAG
    CGAGGCCCGA CGGGCCCACG AGGTGAAAGA GGCCCACGGG GCATCACCGG CAAGCCTGGC
    CCCAAGGGCA ACTCTGGAGG CGATGGCCCG GCCGGCCCTC CAGGTGAACG GGGACCCAAC
    GGACCCCAAG GACCCACGGG GTTCCCTGGA CCAAAGGGCC CCCCGGGGCC CCCAGGCAAG
    GACGGGCTCC CGGGGCACCC TGGACAGCGA GGCGAGACGG GTTTCCAAGG CAAGACCGGC
    CCTCCAGGCC CCCCAGGTGT GGTCGGCCCT CAGGGTCCCA CCGGGGAAAC TGGTCCCATG
    GGCGAGCGTG GCCACCCTGG GCCCCCAGGC CCCCCCGGTG AACAGGGGCT CCCGGGCCTG
    GCCGGGAAAG AAGGGACGAA GGGTGACCCA GGCCCCGCCG GCCTTCCTGG GAAAGACGGT
    CCCCCCGGGC TGCGAGGCTT CCCTGGAGAC CGAGGCCTTC CTGGTCCAGT GGGGGCGCTT
    GGACTGAAAG GCAGTGAAGG CCCCCCCGGC CCACCAGGCC CCGCGGGATC TCCAGGCGAG
    AGAGGTCCAG CCGGTGCTGC TGGGCCCATT GGAATTCCAG GGAGACCCGG GCCCCAGGGG
    CCCCCTGGGC CAGCTGGAGA GAAAGGCGCT CCTGGCGAGA AGGGTCCACA AGGCCCAGCT
    GGCCGAGACG GTCTCCAGGG CCCCGTGGGG CTCCCCGGTC CCGCTGGCCC TGTGGGCCCC
    CCTGGAGAAG ATGGAGACAA GGGAGAGATT GGGGAGCCAG GACAGAAAGG GAGCAAGGGA
    GACAAAGGCG AGCAGGGTCC ACCAGGGCCT ACAGGACCTC AAGGCCCCAT CGGACAGCCA
    GGCCCCTCCG GAGCCGATGG CGAGCCGGGA CCTCGGGGCC AGCAGGGCCT CTTTGGGCAG
    AAAGGCGACG AGGGTCCGAG AGGCTTCCCC GGGCCCCCGG GCCCCGTGGG GCTGCAGGGT
    CTGCCGGGAC CTCCCGGAGA GAAGGGCGAG ACAGGCGACG TGGGTCAGAT GGGCCCGCCA
    GGTCCCCCCG GACCCCGAGG ACCCTCCGGA GCTCCAGGTG CAGACGGGCC ACAAGGACCC
    CCCGGCGGAA TAGGCAACCC TGGAGCCGTG GGAGAGAAGG GTGAACCCGG CGAAGCAGGA
    GAGCCTGGCC TTCCCGGCGA AGGGGGCCCC CCGGGACCCA AAGGCGAAAG GGGGGAGAAA
    GGCGAGTCGG GCCCCTCCGG TGCCGCCGGA CCCCCGGGGC CCAAGGGCCC TCCCGGAGAC
    GACGGTCCCA AAGGCAGCCC CGGCCCCGTC GGTTTTCCCG GAGATCCCGG TCCCCCCGGA
    GAACCCGGCC CCGCGGGTCA AGACGGTCCC CCTGGCGACA AAGGAGACGA TGGTGAACCC
    GGGCAAACGG GATCACCAGG CCCTACGGGT GAGCCAGGCC CATCTGGGCC TCCAGGAAAA
    AGGGGTCCGC CCGGCCCCGC AGGTCCCGAA GGCAGGCAGG GGGAGAAAGG AGCCAAGGGA
    GAAGCCGGCT TGGAAGGCCC TCCTGGGAAG ACTGGCCCCA TTGGCCCCCA GGGGGCCCCC
    GGGAAGCCGG GGCCCGACGG CCTTCGAGGG ATCCCGGGCC CAGTGGGTGA ACAAGGTCTC
    CCCGGAGCCC CGGGCCCTGA TGGCCCCCCA GGACCCATGG GCCCCCCAGG ACTCCCCGGC
    CTCAAAGGAG ACTCTGGACC CAAAGGAGAG AAGGGTCACC CAGGCTTGAT CGGCCTCATC
    GGACCTCCGG GCGAACAGGG AGAAAAGGGT GACCGCGGAG TCCCCGGCCC CCAGGGCTCC
    TCGGGCCCTA AGGGAGAACA GGGTATCACC GGTCCTTCCG GCCCGATTGG CCCCCCGGGT
    CCCCCCGGCC TGCCGGGGCC CCCTGGTCCA AAAGGCGCTA AGGGCTCCTC GGGTCCCACT
    GGCCCGAAGG GTGAGGCAGG CCAGCCAGGA CCCCCTGGCC CACCAGGCCC CCCAGGCGAG
    GTCATCCAGC CCCTGCCGAT CCAGGCATCC AGGACCCGGC GGAATATCGA TGCCAGCCAG
    CTGATGGACG ACGCAGAGGG CGAGCAGTAC ATGGACTATG CGGATGGCAT GGAGGAGATC
    TTCGGCTCGC TCAACTCCCT GAAGCTGGAG ATCGAGCAGA TGAAGCGGCC GCTGGGCACA
    CCGCAGAACC CCGCACGCAC CTGCAAAGAC CTCCAGCTCT GCCACCCCGA CTTCCCTGAC
    GGCGAATACT GGGTGGATCC GAACCAAGGC TGCTCCCGGG ACTCCTTCAA GGTCTACTGC
    AACTTCACGG CCGGGGGGTC AACGTGCGTC TTCCCCGACA AGAAGTCTGA AGGGGCCAGA
    ATCACTTCTT GGCCCAAAGA AAACCCGGGT TCCTGGTTCA GTGAATTCAA GCGTGGCAAA
    CTGCTCTCCT ATGTGGACGC CGAGGGCAAC CCCGTGGGCG TCGTCCAGAT GACCTTCCTG
    CGGCTGCTCA GCGCCTCCGC CCACCAGAAC ATCACCTACA ACTGCTACCA GTCGGTGGCC
    TGGCAGGACG CCGCCACGGG CAGCTATGAC AAGGCCATGC GCTTCCTGGG CTCCAACGAC
    GAGGAGATGT CATATGACAA CAGCCCCTAC ATCCGCGCCC TGGTGGACGG CTGCGCCACG
    AAGAAAGGGT ATCAGAAGAC GGTTCTGGAG ATCGACACGC CCAAGGTGGA GCAGGTGCCC
    ATCGTCGACA TCATGTTCAA CGACTTCGGT GAAGCGTCAC AGAAATTTGG ATTTGAAGTG
    GGGCCGGCTT GCTTCCTGGG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221061
    Clone ID Related Accession (Same CDS sequence) XM_024999727.1
    Accession Version XM_024999727.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-1(V) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGACGTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
    TCCCCGCCGC CGCTGCTGCT GCTGCTGCTG CTGCTGCTGT GGGCGCCGCC TCCGAGCCGC
    GCAGCTCAGC CAGCAGATCT CCTGAAGGTT CTAGATTTTC ACAACCTGCC TGATGGAATA
    ACCAAGACCA CAGGCTTCTG TGCAGCGCGG CGATCTTCCA AAGGCCCGGA TGTCGCCTAC
    AGAGTCACGA AAGATGCCCA GCTCAGCGCG CCCACCAAGC AGCTGTACCC TGCATCTGCG
    TTTCCTGAGG ACTTCTCCAT CCTAACCACT GTGAAAGCCA AGAAAGGCAG CCAGGCCTTC
    CTGGTCTCCA TCTACAATGA GCAGGGCATC CAGCAGATCG GCCTGGAGAT GGGCCGCTCT
    CCGGTCTTCC TCTATGAGGA CCACACGGGG AAGCCGGGGC CAGAGGACTA CCCCCTCTTC
    AGAGGCATCA ACCTGTCAGA TGGCAAGTGG CACAGAATTG CTCTCAGCGT CCACAAGAAG
    AATGTCACCT TGATCCTGGA CTGTAAAAAG AAGACAACCA AGTTCCTGGA CCGCAGTGAA
    CACCCTGTGA TAGACGTCAA CGGCATCGTC GTGTTCGGCA CCCGGATTCT GGATGAGGAA
    GTGTTTGAGG GTGACATCCA GCAGCTGCTC CTCGTCTCGG ACCACCGGGC GGCTTACGAT
    TACTGCGAGC ACTACAGCCC CGACTGTGAC ACGGCCGTCC CCGACAAACC CCAGTCTCAG
    GACCCCAACC CAGATGAATA CTACCCAGAC GGAGACAACG AAGGAGATAC CTATTACTAC
    GAGTACCCCT ACTATGAGGA CACGGACGAC ACAGGCAAAG ACCCCATCAC CACCAAGACG
    CCCGTGGAGG CTGCTCGAGA GACCACGGAG GTGGCTGAGG AGCTGACCCA GCCCCCGACG
    GAAGCCCCTT CGGCGCCCAG CACCACCGAG GGCCCCGGGA AGGAGGAGGA CCCCGGCATC
    GGCGACTACG ACTACGTGCC CAGTGAAGAC TACTACACGC CCCCGCCTTA CGAGGACTTT
    GGCTACGGCG AAGGCATTGA GAACCCGGAT GAGAACCCCG ACCAGCTCCC GGACCAGGGC
    GCCCGGGCCG AGGTTCCCAC CAGCACGGTC CTCACCTCCA ACGACTCCAA CCCGGCCCCG
    CCTCCGGAGG AGGGGATAGA TGACCTGGAG GAAGAGTTCA CGGAAGAAAC CATCAAGAAC
    CTGGACGAGA GCTACTATGA CCCGTACTAC GACCCCACCA TCTCCCCATC GGAGATCGGG
    CCAGGCATGC CTGCCAACCA GGACACCATC TATGAGGGGA TGGGAGGGCC TCGGGGCGAG
    AAAGGTCAAA AAGGAGAACC CGCCATCATC GAACCGGGGA TGCTCCTGGA GGGGCCGCCT
    GGCCCCGAAG GCCCCGCGGG TCTCCCAGGA CCTCCAGGAA CGATGGGTCC CACAGGCCAA
    GTGGGTGACC CTGGAGAAAG AGGTCCCCCT GGACGCCCAG GCCTTCCTGG TGCTGACGGG
    CTGCCCGGCC CTCCTGGAAC CATGCTTATG CTGCCTTTCC GGTTCGGAGG GGGCGGCGAC
    GCGGGCTCCA AAGGACCCAT GGTCTCCGCG CAGGAGTCCC AAGCTCAAGC CATCCTGCAA
    CAGGCCCGGC TGGCGTTGAG GGGGCCTGCT GGCCCAATGG GTCTCACTGG GAGACCGGGT
    CCCATGGGCC CTCCTGGGAG CGGAGGTCTG AAGGGTGAGC CAGGAGACAT GGGGCCTCAG
    GGTCCGCGGG GTGTGCAGGG CCCGCCTGGC CCGGCAGGAA AGCCTGGGAG AAGGGGCCGA
    GCCGGCAGTG ATGGAGCGAG AGGGATGCCT GGGCAGACTG GCCCCAAGGG TGACCGCGGC
    TTCGATGGCC TGGCGGGGCT GCCGGGGGAG AAGGGCCACC GGGGCGACCC TGGCCCGTCC
    GGCCCACCGG GACCTCCGGG GGAAGACGGA GAAAGGGGCG ACGATGGGGA AGTCGGGCCC
    AGAGGGCTGC CTGGGGAGCC CGGGCCGCGA GGTCTGCTGG GGCCGAAGGG GCCCCCAGGC
    CCCCCTGGAC CGCCCGGTGT GACGGGAATG GATGGTCAAC CAGGCCCAAA AGGCAACGTG
    GGTCCCCAGG GAGAGCCCGG CCCCCCGGGG CAGCAGGGCA ACCCAGGTGC CCAGGGTCTT
    CCGGGCCCCC AGGGCGCTAT CGGGCCTCCA GGAGAAAAGG GTCCCCTGGG CAAACCCGGC
    CTCCCAGGAA TGCCTGGTGC TGACGGACCC CCGGGGCACC CTGGCAAAGA AGGCCCTCCA
    GGAGAGAAGG GAGGGCAGGG TCCTCCCGGC CCCCAGGGTC CAATCGGCTA CCCAGGTCCT
    CGCGGAGTCA AGGGGGCAGA CGGCATCCGA GGTCTGAAGG GCACCAAGGG TGAGAAGGGT
    GAAGATGGCT TCCCAGGCTT CAAAGGAGAC ATGGGCATCA AGGGCGATCG GGGGGAGATC
    GGCCCACCTG GTCCCAGAGG GGAGGACGGT CCTGAAGGTC CCAAGGGGCG AGGCGGTCCG
    AACGGCGACC CCGGTCCTCT GGGACCCCCT GGGGAGAAGG GCAAACTCGG AGTCCCAGGA
    CTGCCAGGCT ACCCAGGAAG ACAGGGGCCA AAGGGTTCTA TCGGATTTCC TGGATTTCCC
    GGCGCCAACG GAGAAAAGGG CGGCAGGGGC ACCCCTGGGA AGCCGGGACC ACGGGGGCAG
    CGAGGCCCGA CGGGCCCACG AGGTGAAAGA GGCCCACGGG GCATCACCGG CAAGCCTGGC
    CCCAAGGGCA ACTCTGGAGG CGATGGCCCG GCCGGCCCTC CAGGTGAACG GGGACCCAAC
    GGACCCCAAG GACCCACGGG GTTCCCTGGA CCAAAGGGCC CCCCGGGGCC CCCAGGCAAG
    GACGGGCTCC CGGGGCACCC TGGACAGCGA GGCGAGACGG GTTTCCAAGG CAAGACCGGC
    CCTCCAGGCC CCCCAGGTGT GGTCGGCCCT CAGGGTCCCA CCGGGGAAAC TGGTCCCATG
    GGCGAGCGTG GCCACCCTGG GCCCCCAGGC CCCCCCGGTG AACAGGGGCT CCCGGGCCTG
    GCCGGGAAAG AAGGGACGAA GGGTGACCCA GGCCCCGCCG GCCTTCCTGG GAAAGACGGT
    CCCCCCGGGC TGCGAGGCTT CCCTGGAGAC CGAGGCCTTC CTGGTCCAGT GGGGGCGCTT
    GGACTGAAAG GCAGTGAAGG CCCCCCCGGC CCACCAGGCC CCGCGGGATC TCCAGGCGAG
    AGAGGTCCAG CCGGTGCTGC TGGGCCCATT GGAATTCCAG GGAGACCCGG GCCCCAGGGG
    CCCCCTGGGC CAGCTGGAGA GAAAGGCGCT CCTGGCGAGA AGGGTCCACA AGGCCCAGCT
    GGCCGAGACG GTCTCCAGGG CCCCGTGGGG CTCCCCGGTC CCGCTGGCCC TGTGGGCCCC
    CCTGGAGAAG ATGGAGACAA GGGAGAGATT GGGGAGCCAG GACAGAAAGG GAGCAAGGGA
    GACAAAGGCG AGCAGGGTCC ACCAGGGCCT ACAGGACCTC AAGGCCCCAT CGGACAGCCA
    GGCCCCTCCG GAGCCGATGG CGAGCCGGGA CCTCGGGGCC AGCAGGGCCT CTTTGGGCAG
    AAAGGCGACG AGGGTCCGAG AGGCTTCCCC GGGCCCCCGG GCCCCGTGGG GCTGCAGGGT
    CTGCCGGGAC CTCCCGGAGA GAAGGGCGAG ACAGGCGACG TGGGTCAGAT GGGCCCGCCA
    GGTCCCCCCG GACCCCGAGG ACCCTCCGGA GCTCCAGGTG CAGACGGGCC ACAAGGACCC
    CCCGGCGGAA TAGGCAACCC TGGAGCCGTG GGAGAGAAGG GTGAACCCGG CGAAGCAGGA
    GAGCCTGGCC TTCCCGGCGA AGGGGGCCCC CCGGGACCCA AAGGCGAAAG GGGGGAGAAA
    GGCGAGTCGG GCCCCTCCGG TGCCGCCGGA CCCCCGGGGC CCAAGGGCCC TCCCGGAGAC
    GACGGTCCCA AAGGCAGCCC CGGCCCCGTC GGTTTTCCCG GAGATCCCGG TCCCCCCGGA
    GAACCCGGCC CCGCGGGTCA AGACGGTCCC CCTGGCGACA AAGGAGACGA TGGTGAACCC
    GGGCAAACGG GATCACCAGG CCCTACGGGT GAGCCAGGCC CATCTGGGCC TCCAGGAAAA
    AGGGGTCCGC CCGGCCCCGC AGGTCCCGAA GGCAGGCAGG GGGAGAAAGG AGCCAAGGGA
    GAAGCCGGCT TGGAAGGCCC TCCTGGGAAG ACTGGCCCCA TTGGCCCCCA GGGGGCCCCC
    GGGAAGCCGG GGCCCGACGG CCTTCGAGGG ATCCCGGGCC CAGTGGGTGA ACAAGGTCTC
    CCCGGAGCCC CGGGCCCTGA TGGCCCCCCA GGACCCATGG GCCCCCCAGG ACTCCCCGGC
    CTCAAAGGAG ACTCTGGACC CAAAGGAGAG AAGGGTCACC CAGGCTTGAT CGGCCTCATC
    GGACCTCCGG GCGAACAGGG AGAAAAGGGT GACCGCGGAG TCCCCGGCCC CCAGGGCTCC
    TCGGGCCCTA AGGGAGAACA GGGTATCACC GGTCCTTCCG GCCCGATTGG CCCCCCGGGT
    CCCCCCGGCC TGCCGGGGCC CCCTGGTCCA AAAGGCGCTA AGGGCTCCTC GGGTCCCACT
    GGCCCGAAGG GTGAGGCAGG CCAGCCAGGA CCCCCTGGCC CACCAGGCCC CCCAGGCGAG
    GTCATCCAGC CCCTGCCGAT CCAGGCATCC AGGACCCGGC GGAATATCGA TGCCAGCCAG
    CTGATGGACG ACGCAGAGGG CGAGCAGTAC ATGGACTATG CGGATGGCAT GGAGGAGATC
    TTCGGCTCGC TCAACTCCCT GAAGCTGGAG ATCGAGCAGA TGAAGCGGCC GCTGGGCACA
    CCGCAGAACC CCGCACGCAC CTGCAAAGAC CTCCAGCTCT GCCACCCCGA CTTCCCTGAC
    GGCGAATACT GGGTGGATCC GAACCAAGGC TGCTCCCGGG ACTCCTTCAA GGTCTACTGC
    AACTTCACGG CCGGGGGGTC AACGTGCGTC TTCCCCGACA AGAAGTCTGA AGGGAGTAAA
    ATGGCCCGCT GGCCCAAAGA GCAGCCCTCC ACCTGGTACA GTCAGTACAA GCGAGGGTCG
    CTGCTCTCCT ATGTGGACGC CGAGGGCAAC CCCGTGGGCG TCGTCCAGAT GACCTTCCTG
    CGGCTGCTCA GCGCCTCCGC CCACCAGAAC ATCACCTACA ACTGCTACCA GTCGGTGGCC
    TGGCAGGACG CCGCCACGGG CAGCTATGAC AAGGCCATGC GCTTCCTGGG CTCCAACGAC
    GAGGAGATGT CATATGACAA CAGCCCCTAC ATCCGCGCCC TGGTGGACGG CTGCGCCACG
    AAGAAAGGGT ATCAGAAGAC GGTTCTGGAG ATCGACACGC CCAAGGTGGA GCAGGTGCCC
    ATCGTCGACA TCATGTTCAA CGACTTCGGT GAAGCGTCAC AGAAATTTGG ATTTGAAGTG
    GGGCCGGCTT GCTTCCTGGG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855495.1
    CDS409..5952
    Translation

    Target ORF information:

    RefSeq Version XM_024999727.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 1 chain (COL5A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGACGTCC ACACCCGCTG GAAAGCGCGC AGCCCGCTCC GCCCGGGCGC CCCGCTGCTG 
    TCCCCGCCGC CGCTGCTGCT GCTGCTGCTG CTGCTGCTGT GGGCGCCGCC TCCGAGCCGC
    GCAGCTCAGC CAGCAGATCT CCTGAAGGTT CTAGATTTTC ACAACCTGCC TGATGGAATA
    ACCAAGACCA CAGGCTTCTG TGCAGCGCGG CGATCTTCCA AAGGCCCGGA TGTCGCCTAC
    AGAGTCACGA AAGATGCCCA GCTCAGCGCG CCCACCAAGC AGCTGTACCC TGCATCTGCG
    TTTCCTGAGG ACTTCTCCAT CCTAACCACT GTGAAAGCCA AGAAAGGCAG CCAGGCCTTC
    CTGGTCTCCA TCTACAATGA GCAGGGCATC CAGCAGATCG GCCTGGAGAT GGGCCGCTCT
    CCGGTCTTCC TCTATGAGGA CCACACGGGG AAGCCGGGGC CAGAGGACTA CCCCCTCTTC
    AGAGGCATCA ACCTGTCAGA TGGCAAGTGG CACAGAATTG CTCTCAGCGT CCACAAGAAG
    AATGTCACCT TGATCCTGGA CTGTAAAAAG AAGACAACCA AGTTCCTGGA CCGCAGTGAA
    CACCCTGTGA TAGACGTCAA CGGCATCGTC GTGTTCGGCA CCCGGATTCT GGATGAGGAA
    GTGTTTGAGG GTGACATCCA GCAGCTGCTC CTCGTCTCGG ACCACCGGGC GGCTTACGAT
    TACTGCGAGC ACTACAGCCC CGACTGTGAC ACGGCCGTCC CCGACAAACC CCAGTCTCAG
    GACCCCAACC CAGATGAATA CTACCCAGAC GGAGACAACG AAGGAGATAC CTATTACTAC
    GAGTACCCCT ACTATGAGGA CACGGACGAC ACAGGCAAAG ACCCCATCAC CACCAAGACG
    CCCGTGGAGG CTGCTCGAGA GACCACGGAG GTGGCTGAGG AGCTGACCCA GCCCCCGACG
    GAAGCCCCTT CGGCGCCCAG CACCACCGAG GGCCCCGGGA AGGAGGAGGA CCCCGGCATC
    GGCGACTACG ACTACGTGCC CAGTGAAGAC TACTACACGC CCCCGCCTTA CGAGGACTTT
    GGCTACGGCG AAGGCATTGA GAACCCGGAT GAGAACCCCG ACCAGCTCCC GGACCAGGGC
    GCCCGGGCCG AGGTTCCCAC CAGCACGGTC CTCACCTCCA ACGACTCCAA CCCGGCCCCG
    CCTCCGGAGG AGGGGATAGA TGACCTGGAG GAAGAGTTCA CGGAAGAAAC CATCAAGAAC
    CTGGACGAGA GCTACTATGA CCCGTACTAC GACCCCACCA TCTCCCCATC GGAGATCGGG
    CCAGGCATGC CTGCCAACCA GGACACCATC TATGAGGGGA TGGGAGGGCC TCGGGGCGAG
    AAAGGTCAAA AAGGAGAACC CGCCATCATC GAACCGGGGA TGCTCCTGGA GGGGCCGCCT
    GGCCCCGAAG GCCCCGCGGG TCTCCCAGGA CCTCCAGGAA CGATGGGTCC CACAGGCCAA
    GTGGGTGACC CTGGAGAAAG AGGTCCCCCT GGACGCCCAG GCCTTCCTGG TGCTGACGGG
    CTGCCCGGCC CTCCTGGAAC CATGCTTATG CTGCCTTTCC GGTTCGGAGG GGGCGGCGAC
    GCGGGCTCCA AAGGACCCAT GGTCTCCGCG CAGGAGTCCC AAGCTCAAGC CATCCTGCAA
    CAGGCCCGGC TGGCGTTGAG GGGGCCTGCT GGCCCAATGG GTCTCACTGG GAGACCGGGT
    CCCATGGGCC CTCCTGGGAG CGGAGGTCTG AAGGGTGAGC CAGGAGACAT GGGGCCTCAG
    GGTCCGCGGG GTGTGCAGGG CCCGCCTGGC CCGGCAGGAA AGCCTGGGAG AAGGGGCCGA
    GCCGGCAGTG ATGGAGCGAG AGGGATGCCT GGGCAGACTG GCCCCAAGGG TGACCGCGGC
    TTCGATGGCC TGGCGGGGCT GCCGGGGGAG AAGGGCCACC GGGGCGACCC TGGCCCGTCC
    GGCCCACCGG GACCTCCGGG GGAAGACGGA GAAAGGGGCG ACGATGGGGA AGTCGGGCCC
    AGAGGGCTGC CTGGGGAGCC CGGGCCGCGA GGTCTGCTGG GGCCGAAGGG GCCCCCAGGC
    CCCCCTGGAC CGCCCGGTGT GACGGGAATG GATGGTCAAC CAGGCCCAAA AGGCAACGTG
    GGTCCCCAGG GAGAGCCCGG CCCCCCGGGG CAGCAGGGCA ACCCAGGTGC CCAGGGTCTT
    CCGGGCCCCC AGGGCGCTAT CGGGCCTCCA GGAGAAAAGG GTCCCCTGGG CAAACCCGGC
    CTCCCAGGAA TGCCTGGTGC TGACGGACCC CCGGGGCACC CTGGCAAAGA AGGCCCTCCA
    GGAGAGAAGG GAGGGCAGGG TCCTCCCGGC CCCCAGGGTC CAATCGGCTA CCCAGGTCCT
    CGCGGAGTCA AGGGGGCAGA CGGCATCCGA GGTCTGAAGG GCACCAAGGG TGAGAAGGGT
    GAAGATGGCT TCCCAGGCTT CAAAGGAGAC ATGGGCATCA AGGGCGATCG GGGGGAGATC
    GGCCCACCTG GTCCCAGAGG GGAGGACGGT CCTGAAGGTC CCAAGGGGCG AGGCGGTCCG
    AACGGCGACC CCGGTCCTCT GGGACCCCCT GGGGAGAAGG GCAAACTCGG AGTCCCAGGA
    CTGCCAGGCT ACCCAGGAAG ACAGGGGCCA AAGGGTTCTA TCGGATTTCC TGGATTTCCC
    GGCGCCAACG GAGAAAAGGG CGGCAGGGGC ACCCCTGGGA AGCCGGGACC ACGGGGGCAG
    CGAGGCCCGA CGGGCCCACG AGGTGAAAGA GGCCCACGGG GCATCACCGG CAAGCCTGGC
    CCCAAGGGCA ACTCTGGAGG CGATGGCCCG GCCGGCCCTC CAGGTGAACG GGGACCCAAC
    GGACCCCAAG GACCCACGGG GTTCCCTGGA CCAAAGGGCC CCCCGGGGCC CCCAGGCAAG
    GACGGGCTCC CGGGGCACCC TGGACAGCGA GGCGAGACGG GTTTCCAAGG CAAGACCGGC
    CCTCCAGGCC CCCCAGGTGT GGTCGGCCCT CAGGGTCCCA CCGGGGAAAC TGGTCCCATG
    GGCGAGCGTG GCCACCCTGG GCCCCCAGGC CCCCCCGGTG AACAGGGGCT CCCGGGCCTG
    GCCGGGAAAG AAGGGACGAA GGGTGACCCA GGCCCCGCCG GCCTTCCTGG GAAAGACGGT
    CCCCCCGGGC TGCGAGGCTT CCCTGGAGAC CGAGGCCTTC CTGGTCCAGT GGGGGCGCTT
    GGACTGAAAG GCAGTGAAGG CCCCCCCGGC CCACCAGGCC CCGCGGGATC TCCAGGCGAG
    AGAGGTCCAG CCGGTGCTGC TGGGCCCATT GGAATTCCAG GGAGACCCGG GCCCCAGGGG
    CCCCCTGGGC CAGCTGGAGA GAAAGGCGCT CCTGGCGAGA AGGGTCCACA AGGCCCAGCT
    GGCCGAGACG GTCTCCAGGG CCCCGTGGGG CTCCCCGGTC CCGCTGGCCC TGTGGGCCCC
    CCTGGAGAAG ATGGAGACAA GGGAGAGATT GGGGAGCCAG GACAGAAAGG GAGCAAGGGA
    GACAAAGGCG AGCAGGGTCC ACCAGGGCCT ACAGGACCTC AAGGCCCCAT CGGACAGCCA
    GGCCCCTCCG GAGCCGATGG CGAGCCGGGA CCTCGGGGCC AGCAGGGCCT CTTTGGGCAG
    AAAGGCGACG AGGGTCCGAG AGGCTTCCCC GGGCCCCCGG GCCCCGTGGG GCTGCAGGGT
    CTGCCGGGAC CTCCCGGAGA GAAGGGCGAG ACAGGCGACG TGGGTCAGAT GGGCCCGCCA
    GGTCCCCCCG GACCCCGAGG ACCCTCCGGA GCTCCAGGTG CAGACGGGCC ACAAGGACCC
    CCCGGCGGAA TAGGCAACCC TGGAGCCGTG GGAGAGAAGG GTGAACCCGG CGAAGCAGGA
    GAGCCTGGCC TTCCCGGCGA AGGGGGCCCC CCGGGACCCA AAGGCGAAAG GGGGGAGAAA
    GGCGAGTCGG GCCCCTCCGG TGCCGCCGGA CCCCCGGGGC CCAAGGGCCC TCCCGGAGAC
    GACGGTCCCA AAGGCAGCCC CGGCCCCGTC GGTTTTCCCG GAGATCCCGG TCCCCCCGGA
    GAACCCGGCC CCGCGGGTCA AGACGGTCCC CCTGGCGACA AAGGAGACGA TGGTGAACCC
    GGGCAAACGG GATCACCAGG CCCTACGGGT GAGCCAGGCC CATCTGGGCC TCCAGGAAAA
    AGGGGTCCGC CCGGCCCCGC AGGTCCCGAA GGCAGGCAGG GGGAGAAAGG AGCCAAGGGA
    GAAGCCGGCT TGGAAGGCCC TCCTGGGAAG ACTGGCCCCA TTGGCCCCCA GGGGGCCCCC
    GGGAAGCCGG GGCCCGACGG CCTTCGAGGG ATCCCGGGCC CAGTGGGTGA ACAAGGTCTC
    CCCGGAGCCC CGGGCCCTGA TGGCCCCCCA GGACCCATGG GCCCCCCAGG ACTCCCCGGC
    CTCAAAGGAG ACTCTGGACC CAAAGGAGAG AAGGGTCACC CAGGCTTGAT CGGCCTCATC
    GGACCTCCGG GCGAACAGGG AGAAAAGGGT GACCGCGGAG TCCCCGGCCC CCAGGGCTCC
    TCGGGCCCTA AGGGAGAACA GGGTATCACC GGTCCTTCCG GCCCGATTGG CCCCCCGGGT
    CCCCCCGGCC TGCCGGGGCC CCCTGGTCCA AAAGGCGCTA AGGGCTCCTC GGGTCCCACT
    GGCCCGAAGG GTGAGGCAGG CCAGCCAGGA CCCCCTGGCC CACCAGGCCC CCCAGGCGAG
    GTCATCCAGC CCCTGCCGAT CCAGGCATCC AGGACCCGGC GGAATATCGA TGCCAGCCAG
    CTGATGGACG ACGCAGAGGG CGAGCAGTAC ATGGACTATG CGGATGGCAT GGAGGAGATC
    TTCGGCTCGC TCAACTCCCT GAAGCTGGAG ATCGAGCAGA TGAAGCGGCC GCTGGGCACA
    CCGCAGAACC CCGCACGCAC CTGCAAAGAC CTCCAGCTCT GCCACCCCGA CTTCCCTGAC
    GGCGAATACT GGGTGGATCC GAACCAAGGC TGCTCCCGGG ACTCCTTCAA GGTCTACTGC
    AACTTCACGG CCGGGGGGTC AACGTGCGTC TTCCCCGACA AGAAGTCTGA AGGGAGTAAA
    ATGGCCCGCT GGCCCAAAGA GCAGCCCTCC ACCTGGTACA GTCAGTACAA GCGAGGGTCG
    CTGCTCTCCT ATGTGGACGC CGAGGGCAAC CCCGTGGGCG TCGTCCAGAT GACCTTCCTG
    CGGCTGCTCA GCGCCTCCGC CCACCAGAAC ATCACCTACA ACTGCTACCA GTCGGTGGCC
    TGGCAGGACG CCGCCACGGG CAGCTATGAC AAGGCCATGC GCTTCCTGGG CTCCAACGAC
    GAGGAGATGT CATATGACAA CAGCCCCTAC ATCCGCGCCC TGGTGGACGG CTGCGCCACG
    AAGAAAGGGT ATCAGAAGAC GGTTCTGGAG ATCGACACGC CCAAGGTGGA GCAGGTGCCC
    ATCGTCGACA TCATGTTCAA CGACTTCGGT GAAGCGTCAC AGAAATTTGG ATTTGAAGTG
    GGGCCGGCTT GCTTCCTGGG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92066
    Clone ID Related Accession (Same CDS sequence) XM_015465612.1 , XM_015473738.1
    Accession Version XM_015465612.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: collagen alpha-1(V) chain
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCTGCCA ACCAGGACAC CATCTATGAG GGGATGGGAG GGCCTCGGGG CGAGAAAGGT 
    CAAAAAGGAG AACCCGCCAT CATCGAACCG GGGATGCTCC TGGAGGGGCC GCCTGGCCCC
    GAAGGCCCCG CGGGTCTCCC AGGACCTCCA GGAACGATGG GTCCCACAGG CCAAGTGGGT
    GACCCTGGAG AAAGAGGTCC CCCTGGACGC CCAGGCCTTC CTGGTGCTGA CGGGCTGCCC
    GGCCCTCCTG GAACCATGCT TATGCTGCCT TTCCGGTTCG GAGGGGGCGG CGACGCGGGC
    TCCAAAGGAC CCATGGTCTC CGCGCAGGAG TCCCAAGCTC AAGCCATCCT GCAACAGGCC
    CGGCTGGCGT TGAGGGGGCC TGCTGGCCCA ATGGGTCTCA CTGGGAGACC GGGTCCCATG
    GGCCCTCCTG GGAGCGGAGG TCTGAAGGGT GAGCCAGGAG ACATGGGGCC TCAGGGTCCG
    CGGGGTGTGC AGGGCCCGCC TGGCCCGGCA GGAAAGCCTG GGAGAAGGGG CCGAGCCGGC
    AGTGATGGAG CGAGAGGGAT GCCTGGGCAG ACTGGCCCCA AGGGTGACCG CGGCTTCGAT
    GGCCTGGCGG GGCTGCCGGG GGAGAAGGGC CACCGGGGCG ACCCTGGCCC GTCCGGCCCA
    CCGGGACCTC CGGGGGAAGA CGGAGAAAGG GGCGACGATG GGGAAGTCGG GCCCAGAGGG
    CTGCCTGGGG AGCCCGGGCC GCGAGGTCTG CTGGGGCCGA AGGGGCCCCC AGGCCCCCCT
    GGACCGCCCG GTGTGACGGG AATGGATGGT CAACCAGGCC CAAAAGGCAA CGTGGGTCCC
    CAGGGAGAGC CCGGCCCCCC GGGGCAGCAG GGCAACCCAG GTGCCCAGGG TCTTCCGGGC
    CCCCAGGGCG CTATCGGGCC TCCAGGAGAA AAGGGTCCCC TGGGCAAACC CGGCCTCCCA
    GGAATGCCTG GTGCTGACGG ACCCCCGGGG CACCCTGGCA AAGAAGGCCC TCCAGGAGAG
    AAGGGAGGGC AGGGTCCTCC CGGCCCCCAG GGTCCAATCG GCTACCCAGG TCCTCGCGGA
    GTCAAGGGGG CAGACGGCAT CCGAGGTCTG AAGGGCACCA AGGGTGAGAA GGGTGAAGAT
    GGCTTCCCAG GCTTCAAAGG AGACATGGGC ATCAAGGGCG ATCGGGGGGA GATCGGCCCA
    CCTGGTCCAN GAGGGGAGGA CGGTCCTGAA GGTCCCAAGG GGCGAGGCGG TCCGAACGGC
    GACCCCGGTC CTCTGGGACC CCCTGGGGAG AAGGGCAAAC TCGGAGTCCC AGGACTGCCA
    GGCTACCCAG GAAGACAGGG GCCAAAGGGT TCTATCGGAT TTCCTGGATT TCCCGGCGCC
    AACGGAGAAA AGGGCGGCAG GGGCACCCCT GGGAAGCCGG GACCACGGGG GCAGCGAGGC
    CCGACGGGCC CACGAGGTGA AAGAGGCCCA CGGGGCATCA CCGGCAAGCC TGGCCCCAAG
    GGCAACTCTG GAGGCGATGG CCCGGCCGGC CCTCCAGGTG AACGGGGACC CAACGGACCC
    CAAGGACCCA CGGGGTTCCC TGGACCAAAG GGCCCCCCGG GGCCCCCAGG CAAGGACGGG
    CTCCCGGGGC ACCCTGGACA GCGAGGCGAG ACGGGTTTCC AAGGCAAGAC CGGCCCTCCA
    GGCCCCCCAG GTGTGGTCGG CCCTCAGGGT CCCACCGGGG AAACTGGTCC CATGGGCGAG
    CGTGGCCACC CTGGGCCCCC AGGCCCCCCC GGTGAACAGG GGCTCCCGGG CCTGGCCGGG
    AAAGAAGGGA CGAAGGGTGA CCCAGGCCCC GCCGGCCTTC CTGGGAAAGA CGGTCCCCCC
    GGGCTGCGAG GCTTCCCTGG AGACCGAGGC CTTCCTGGTC CAGTGGGGGC GCTTGGACTG
    AAAGGCAGTG AAGGCCCCCC CGGCCCACCA GGCCCCGCGG GATCTCCAGG CGAGAGAGGT
    CCAGCCGGTG CTGCTGGGCC CATTGGAATT CCAGGGAGAC CCGGGCCCCA GGGGCCCCCT
    GGGCCAGCTG GAGAGAAAGG CGCTCCTGGC GAGAAGGGTC CACAAGGCCC AGCTGGCCGA
    GACGGTCTCC AGGGCCCCGT GGGGCTCCCC GGTCCCGCTG GCCCTGTGGG CCCCCCTGGA
    GAAGATGGAG ACAAGGGAGA GATTGGGGAG CCAGGACAGA AAGGGAGCAA GGGAGACAAA
    GGCGAGCAGG GTCCACCAGG GCCTACAGGA CCTCAAGGCC CCATCGGACA GCCAGGCCCC
    TCCGGAGCCG ATGGCGAGCC GGGACCTCGG GGCCAGCAGG GCCTCTTTGG GCAGAAAGGC
    GACGAGGGTC CGAGAGGCTT CCCCGGGCCC CCGGGCCCCG TGGGGCTGCA GGGTCTGCCG
    GGACCTCCCG GAGAGAAGGG CGAGACAGGC GACGTGGGTC AGATGGGCCC GCCAGGTCCC
    CCCGGACCCC GAGGACCCTC CGGAGCTCCA GGTGCAGACG GGCCACAAGG ACCCCCCGGC
    GGAATAGGCA ACCCTGGAGC CGTGGGAGAG AAGGGTGAAC CCGGCGAAGC AGGAGAGCCT
    GGCCTTCCCG GCGAAGGGGG CCCCCCGGTG AGTGAGCGGG CTCTGGGGAG GGGTGGGGCG
    TGGCATGGAC GGCTGCCGGG GGGCAGGGTC CCACTCTACC TCAGCTTTCC CATCCGTGAA
    ATGGGAGTCG GGCCTCCTCT TGTGCCTCCA TCACGAGACT GCTGGAAAAA AGAGGGAGTG
    TGGTGGGGAG TGAGTGTGTG TGTGACAGAG AGTGCCTGTG TGTGTGCGTG TGTGTGCGCG
    TGTGTGGAGG GTCTGCAGAG CATCGAGTCC TACAGTTCAG GGTTCAGGAT CAGGGTCAGC
    CACGGCCCCA GGAGCAGACA CCTGGGCAGG AGGCTGCCCT CTGAGGCCTC TGGAAGGCTG
    GCGGGAGGCA TGGGCCGGCT GTGTGGACGC AGCCCTGTGA GTCCAGCGCG CTCTGGGAGG
    GTGGCTGACG CTTCTCTCGC TCCATTTCAG GGCCCCGTCG GTTTTCCCGG AGATCCCGGT
    CCCCCCGGAG AACCCGGCCC CGCGGGTCAA GACGGTCCCC CTGGCGACAA AGGAGACGAT
    GGTGAACCCG GGCAAACGGG ATCACCAGGC CCTACGGGTG AGCCAGGCCC ATCTGGGCCT
    CCAGGAAAAA GGGGTCCGCC CGGCCCCGCA GGTCCCGAAG GCAGGCAGGG GGAGAAAGGA
    GCCAAGGGAG AAGCCGGCTT GGAAGGCCCT CCTGGGAAGA CTGGCCCCAT TGGCCCCCAG
    GGGGCCCCCG GGAAGCCGGG GCCCGACGGC CTTCGAGGGA TCCCGGGCCC AGTGGGTGAA
    CAAGGTCTCC CCGGAGCCCC GGGCCCTGAT GGCCCCCCAG GACCCATGGG CCCCCCAGGA
    CTCCCCGGCC TCAAAGGAGA CTCTGGACCC AAAGGAGAGA AGGGTCACCC AGGCTTGATC
    GGCCTCATCG GACCTCCGGG CGAACAGGGA GAAAAGGGTG ACCGCGGAGT CCCCGGCCCC
    CAGGGCTCCT CGGGCCCTAA GGGAGAACAG GGTATCACCG GTCCTTCCGG CCCGATTGGC
    CCCCCGGGTC CCCCCGGCCT GCCGGGGCCC CCTGGTCCAA AAGGCGCTAA GGGCTCCTCG
    GGTCCCACTG GCCCGAAGGG TGAGGCAGGC CAGCCAGGAC CCCCTGGCCC ACCAGGCCCC
    CCAGGCGAGG TCATCCAGCC CCTGCCGATC CAGGCATCCA GGACCCGGCG GAATATCGAT
    GCCAGCCAGC TGATGGACGA CGCAGAGGGC GAGCAGTACA TGGACTATGC GGATGGCATG
    GAGGAGATCT TCGGCTCGCT CAACTCCCTG AAGCTGGAGA TCGAGCAGAT GAAGCGGCCG
    CTGGGCACAC CGCAGAACCC CGCACGCACC TGCAAAGACC TCCAGCTCTG CCACCCCGAC
    TTCCCTGACG GCGAATACTG GGTGGATCCG AACCAAGGCT GCTCCCGGGA CTCCTTCAAG
    GTCTACTGCA ACTTCACGGC CGGGGGGTCA ACGTGCGTCT TCCCCGACAA GAAGTCTGAA
    GGGGCCAGAA TCACTTCTTG GCCCAAAGAA AACCCGGGTT CCTGGTTCAG TGAATTCAAG
    CGTGGCAAAC TGCTCTCCTA TGTGGACGCC GAGGGCAACC CCGTGGGCGT CGTCCAGATG
    ACCTTCCTGC GGCTGCTCAG CGCCTCCGCC CACCAGAACA TCACCTACAA CTGCTACCAG
    TCGGTGGCCT GGCAGGACGC CGCCACGGGC AGCTATGACA AGGCCATGCG CTTCCTGGGC
    TCCAACGACG AGGAGATGTC ATATGACAAC AGCCCCTACA TCCGCGCCCT GGTGGACGGC
    TGCGCCACGA AGAAAGGGTA TCAGAAGACG GTTCTGGAGA TCGACACGCC CAAGGTGGAG
    CAGGTGCCCA TCGTCGACAT CATGTTCAAC GACTTCGGTG AAGCGTCACA GAAATTTGGA
    TTTGAAGTGG GGCCGGCTTG CTTCCTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015321098.1
    CDS417..4949
    Translation

    Target ORF information:

    RefSeq Version XM_015465612.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 1 (COL5A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465612.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCTGCCA ACCAGGACAC CATCTATGAG GGGATGGGAG GGCCTCGGGG CGAGAAAGGT 
    CAAAAAGGAG AACCCGCCAT CATCGAACCG GGGATGCTCC TGGAGGGGCC GCCTGGCCCC
    GAAGGCCCCG CGGGTCTCCC AGGACCTCCA GGAACGATGG GTCCCACAGG CCAAGTGGGT
    GACCCTGGAG AAAGAGGTCC CCCTGGACGC CCAGGCCTTC CTGGTGCTGA CGGGCTGCCC
    GGCCCTCCTG GAACCATGCT TATGCTGCCT TTCCGGTTCG GAGGGGGCGG CGACGCGGGC
    TCCAAAGGAC CCATGGTCTC CGCGCAGGAG TCCCAAGCTC AAGCCATCCT GCAACAGGCC
    CGGCTGGCGT TGAGGGGGCC TGCTGGCCCA ATGGGTCTCA CTGGGAGACC GGGTCCCATG
    GGCCCTCCTG GGAGCGGAGG TCTGAAGGGT GAGCCAGGAG ACATGGGGCC TCAGGGTCCG
    CGGGGTGTGC AGGGCCCGCC TGGCCCGGCA GGAAAGCCTG GGAGAAGGGG CCGAGCCGGC
    AGTGATGGAG CGAGAGGGAT GCCTGGGCAG ACTGGCCCCA AGGGTGACCG CGGCTTCGAT
    GGCCTGGCGG GGCTGCCGGG GGAGAAGGGC CACCGGGGCG ACCCTGGCCC GTCCGGCCCA
    CCGGGACCTC CGGGGGAAGA CGGAGAAAGG GGCGACGATG GGGAAGTCGG GCCCAGAGGG
    CTGCCTGGGG AGCCCGGGCC GCGAGGTCTG CTGGGGCCGA AGGGGCCCCC AGGCCCCCCT
    GGACCGCCCG GTGTGACGGG AATGGATGGT CAACCAGGCC CAAAAGGCAA CGTGGGTCCC
    CAGGGAGAGC CCGGCCCCCC GGGGCAGCAG GGCAACCCAG GTGCCCAGGG TCTTCCGGGC
    CCCCAGGGCG CTATCGGGCC TCCAGGAGAA AAGGGTCCCC TGGGCAAACC CGGCCTCCCA
    GGAATGCCTG GTGCTGACGG ACCCCCGGGG CACCCTGGCA AAGAAGGCCC TCCAGGAGAG
    AAGGGAGGGC AGGGTCCTCC CGGCCCCCAG GGTCCAATCG GCTACCCAGG TCCTCGCGGA
    GTCAAGGGGG CAGACGGCAT CCGAGGTCTG AAGGGCACCA AGGGTGAGAA GGGTGAAGAT
    GGCTTCCCAG GCTTCAAAGG AGACATGGGC ATCAAGGGCG ATCGGGGGGA GATCGGCCCA
    CCTGGTCCAN GAGGGGAGGA CGGTCCTGAA GGTCCCAAGG GGCGAGGCGG TCCGAACGGC
    GACCCCGGTC CTCTGGGACC CCCTGGGGAG AAGGGCAAAC TCGGAGTCCC AGGACTGCCA
    GGCTACCCAG GAAGACAGGG GCCAAAGGGT TCTATCGGAT TTCCTGGATT TCCCGGCGCC
    AACGGAGAAA AGGGCGGCAG GGGCACCCCT GGGAAGCCGG GACCACGGGG GCAGCGAGGC
    CCGACGGGCC CACGAGGTGA AAGAGGCCCA CGGGGCATCA CCGGCAAGCC TGGCCCCAAG
    GGCAACTCTG GAGGCGATGG CCCGGCCGGC CCTCCAGGTG AACGGGGACC CAACGGACCC
    CAAGGACCCA CGGGGTTCCC TGGACCAAAG GGCCCCCCGG GGCCCCCAGG CAAGGACGGG
    CTCCCGGGGC ACCCTGGACA GCGAGGCGAG ACGGGTTTCC AAGGCAAGAC CGGCCCTCCA
    GGCCCCCCAG GTGTGGTCGG CCCTCAGGGT CCCACCGGGG AAACTGGTCC CATGGGCGAG
    CGTGGCCACC CTGGGCCCCC AGGCCCCCCC GGTGAACAGG GGCTCCCGGG CCTGGCCGGG
    AAAGAAGGGA CGAAGGGTGA CCCAGGCCCC GCCGGCCTTC CTGGGAAAGA CGGTCCCCCC
    GGGCTGCGAG GCTTCCCTGG AGACCGAGGC CTTCCTGGTC CAGTGGGGGC GCTTGGACTG
    AAAGGCAGTG AAGGCCCCCC CGGCCCACCA GGCCCCGCGG GATCTCCAGG CGAGAGAGGT
    CCAGCCGGTG CTGCTGGGCC CATTGGAATT CCAGGGAGAC CCGGGCCCCA GGGGCCCCCT
    GGGCCAGCTG GAGAGAAAGG CGCTCCTGGC GAGAAGGGTC CACAAGGCCC AGCTGGCCGA
    GACGGTCTCC AGGGCCCCGT GGGGCTCCCC GGTCCCGCTG GCCCTGTGGG CCCCCCTGGA
    GAAGATGGAG ACAAGGGAGA GATTGGGGAG CCAGGACAGA AAGGGAGCAA GGGAGACAAA
    GGCGAGCAGG GTCCACCAGG GCCTACAGGA CCTCAAGGCC CCATCGGACA GCCAGGCCCC
    TCCGGAGCCG ATGGCGAGCC GGGACCTCGG GGCCAGCAGG GCCTCTTTGG GCAGAAAGGC
    GACGAGGGTC CGAGAGGCTT CCCCGGGCCC CCGGGCCCCG TGGGGCTGCA GGGTCTGCCG
    GGACCTCCCG GAGAGAAGGG CGAGACAGGC GACGTGGGTC AGATGGGCCC GCCAGGTCCC
    CCCGGACCCC GAGGACCCTC CGGAGCTCCA GGTGCAGACG GGCCACAAGG ACCCCCCGGC
    GGAATAGGCA ACCCTGGAGC CGTGGGAGAG AAGGGTGAAC CCGGCGAAGC AGGAGAGCCT
    GGCCTTCCCG GCGAAGGGGG CCCCCCGGTG AGTGAGCGGG CTCTGGGGAG GGGTGGGGCG
    TGGCATGGAC GGCTGCCGGG GGGCAGGGTC CCACTCTACC TCAGCTTTCC CATCCGTGAA
    ATGGGAGTCG GGCCTCCTCT TGTGCCTCCA TCACGAGACT GCTGGAAAAA AGAGGGAGTG
    TGGTGGGGAG TGAGTGTGTG TGTGACAGAG AGTGCCTGTG TGTGTGCGTG TGTGTGCGCG
    TGTGTGGAGG GTCTGCAGAG CATCGAGTCC TACAGTTCAG GGTTCAGGAT CAGGGTCAGC
    CACGGCCCCA GGAGCAGACA CCTGGGCAGG AGGCTGCCCT CTGAGGCCTC TGGAAGGCTG
    GCGGGAGGCA TGGGCCGGCT GTGTGGACGC AGCCCTGTGA GTCCAGCGCG CTCTGGGAGG
    GTGGCTGACG CTTCTCTCGC TCCATTTCAG GGCCCCGTCG GTTTTCCCGG AGATCCCGGT
    CCCCCCGGAG AACCCGGCCC CGCGGGTCAA GACGGTCCCC CTGGCGACAA AGGAGACGAT
    GGTGAACCCG GGCAAACGGG ATCACCAGGC CCTACGGGTG AGCCAGGCCC ATCTGGGCCT
    CCAGGAAAAA GGGGTCCGCC CGGCCCCGCA GGTCCCGAAG GCAGGCAGGG GGAGAAAGGA
    GCCAAGGGAG AAGCCGGCTT GGAAGGCCCT CCTGGGAAGA CTGGCCCCAT TGGCCCCCAG
    GGGGCCCCCG GGAAGCCGGG GCCCGACGGC CTTCGAGGGA TCCCGGGCCC AGTGGGTGAA
    CAAGGTCTCC CCGGAGCCCC GGGCCCTGAT GGCCCCCCAG GACCCATGGG CCCCCCAGGA
    CTCCCCGGCC TCAAAGGAGA CTCTGGACCC AAAGGAGAGA AGGGTCACCC AGGCTTGATC
    GGCCTCATCG GACCTCCGGG CGAACAGGGA GAAAAGGGTG ACCGCGGAGT CCCCGGCCCC
    CAGGGCTCCT CGGGCCCTAA GGGAGAACAG GGTATCACCG GTCCTTCCGG CCCGATTGGC
    CCCCCGGGTC CCCCCGGCCT GCCGGGGCCC CCTGGTCCAA AAGGCGCTAA GGGCTCCTCG
    GGTCCCACTG GCCCGAAGGG TGAGGCAGGC CAGCCAGGAC CCCCTGGCCC ACCAGGCCCC
    CCAGGCGAGG TCATCCAGCC CCTGCCGATC CAGGCATCCA GGACCCGGCG GAATATCGAT
    GCCAGCCAGC TGATGGACGA CGCAGAGGGC GAGCAGTACA TGGACTATGC GGATGGCATG
    GAGGAGATCT TCGGCTCGCT CAACTCCCTG AAGCTGGAGA TCGAGCAGAT GAAGCGGCCG
    CTGGGCACAC CGCAGAACCC CGCACGCACC TGCAAAGACC TCCAGCTCTG CCACCCCGAC
    TTCCCTGACG GCGAATACTG GGTGGATCCG AACCAAGGCT GCTCCCGGGA CTCCTTCAAG
    GTCTACTGCA ACTTCACGGC CGGGGGGTCA ACGTGCGTCT TCCCCGACAA GAAGTCTGAA
    GGGGCCAGAA TCACTTCTTG GCCCAAAGAA AACCCGGGTT CCTGGTTCAG TGAATTCAAG
    CGTGGCAAAC TGCTCTCCTA TGTGGACGCC GAGGGCAACC CCGTGGGCGT CGTCCAGATG
    ACCTTCCTGC GGCTGCTCAG CGCCTCCGCC CACCAGAACA TCACCTACAA CTGCTACCAG
    TCGGTGGCCT GGCAGGACGC CGCCACGGGC AGCTATGACA AGGCCATGCG CTTCCTGGGC
    TCCAACGACG AGGAGATGTC ATATGACAAC AGCCCCTACA TCCGCGCCCT GGTGGACGGC
    TGCGCCACGA AGAAAGGGTA TCAGAAGACG GTTCTGGAGA TCGACACGCC CAAGGTGGAG
    CAGGTGCCCA TCGTCGACAT CATGTTCAAC GACTTCGGTG AAGCGTCACA GAAATTTGGA
    TTTGAAGTGG GGCCGGCTTG CTTCCTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92066
    Clone ID Related Accession (Same CDS sequence) XM_015465612.1 , XM_015473738.1
    Accession Version XM_015473738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: collagen alpha-1(V) chain
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104305.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCTGCCA ACCAGGACAC CATCTATGAG GGGATGGGAG GGCCTCGGGG CGAGAAAGGT 
    CAAAAAGGAG AACCCGCCAT CATCGAACCG GGGATGCTCC TGGAGGGGCC GCCTGGCCCC
    GAAGGCCCCG CGGGTCTCCC AGGACCTCCA GGAACGATGG GTCCCACAGG CCAAGTGGGT
    GACCCTGGAG AAAGAGGTCC CCCTGGACGC CCAGGCCTTC CTGGTGCTGA CGGGCTGCCC
    GGCCCTCCTG GAACCATGCT TATGCTGCCT TTCCGGTTCG GAGGGGGCGG CGACGCGGGC
    TCCAAAGGAC CCATGGTCTC CGCGCAGGAG TCCCAAGCTC AAGCCATCCT GCAACAGGCC
    CGGCTGGCGT TGAGGGGGCC TGCTGGCCCA ATGGGTCTCA CTGGGAGACC GGGTCCCATG
    GGCCCTCCTG GGAGCGGAGG TCTGAAGGGT GAGCCAGGAG ACATGGGGCC TCAGGGTCCG
    CGGGGTGTGC AGGGCCCGCC TGGCCCGGCA GGAAAGCCTG GGAGAAGGGG CCGAGCCGGC
    AGTGATGGAG CGAGAGGGAT GCCTGGGCAG ACTGGCCCCA AGGGTGACCG CGGCTTCGAT
    GGCCTGGCGG GGCTGCCGGG GGAGAAGGGC CACCGGGGCG ACCCTGGCCC GTCCGGCCCA
    CCGGGACCTC CGGGGGAAGA CGGAGAAAGG GGCGACGATG GGGAAGTCGG GCCCAGAGGG
    CTGCCTGGGG AGCCCGGGCC GCGAGGTCTG CTGGGGCCGA AGGGGCCCCC AGGCCCCCCT
    GGACCGCCCG GTGTGACGGG AATGGATGGT CAACCAGGCC CAAAAGGCAA CGTGGGTCCC
    CAGGGAGAGC CCGGCCCCCC GGGGCAGCAG GGCAACCCAG GTGCCCAGGG TCTTCCGGGC
    CCCCAGGGCG CTATCGGGCC TCCAGGAGAA AAGGGTCCCC TGGGCAAACC CGGCCTCCCA
    GGAATGCCTG GTGCTGACGG ACCCCCGGGG CACCCTGGCA AAGAAGGCCC TCCAGGAGAG
    AAGGGAGGGC AGGGTCCTCC CGGCCCCCAG GGTCCAATCG GCTACCCAGG TCCTCGCGGA
    GTCAAGGGGG CAGACGGCAT CCGAGGTCTG AAGGGCACCA AGGGTGAGAA GGGTGAAGAT
    GGCTTCCCAG GCTTCAAAGG AGACATGGGC ATCAAGGGCG ATCGGGGGGA GATCGGCCCA
    CCTGGTCCAN GAGGGGAGGA CGGTCCTGAA GGTCCCAAGG GGCGAGGCGG TCCGAACGGC
    GACCCCGGTC CTCTGGGACC CCCTGGGGAG AAGGGCAAAC TCGGAGTCCC AGGACTGCCA
    GGCTACCCAG GAAGACAGGG GCCAAAGGGT TCTATCGGAT TTCCTGGATT TCCCGGCGCC
    AACGGAGAAA AGGGCGGCAG GGGCACCCCT GGGAAGCCGG GACCACGGGG GCAGCGAGGC
    CCGACGGGCC CACGAGGTGA AAGAGGCCCA CGGGGCATCA CCGGCAAGCC TGGCCCCAAG
    GGCAACTCTG GAGGCGATGG CCCGGCCGGC CCTCCAGGTG AACGGGGACC CAACGGACCC
    CAAGGACCCA CGGGGTTCCC TGGACCAAAG GGCCCCCCGG GGCCCCCAGG CAAGGACGGG
    CTCCCGGGGC ACCCTGGACA GCGAGGCGAG ACGGGTTTCC AAGGCAAGAC CGGCCCTCCA
    GGCCCCCCAG GTGTGGTCGG CCCTCAGGGT CCCACCGGGG AAACTGGTCC CATGGGCGAG
    CGTGGCCACC CTGGGCCCCC AGGCCCCCCC GGTGAACAGG GGCTCCCGGG CCTGGCCGGG
    AAAGAAGGGA CGAAGGGTGA CCCAGGCCCC GCCGGCCTTC CTGGGAAAGA CGGTCCCCCC
    GGGCTGCGAG GCTTCCCTGG AGACCGAGGC CTTCCTGGTC CAGTGGGGGC GCTTGGACTG
    AAAGGCAGTG AAGGCCCCCC CGGCCCACCA GGCCCCGCGG GATCTCCAGG CGAGAGAGGT
    CCAGCCGGTG CTGCTGGGCC CATTGGAATT CCAGGGAGAC CCGGGCCCCA GGGGCCCCCT
    GGGCCAGCTG GAGAGAAAGG CGCTCCTGGC GAGAAGGGTC CACAAGGCCC AGCTGGCCGA
    GACGGTCTCC AGGGCCCCGT GGGGCTCCCC GGTCCCGCTG GCCCTGTGGG CCCCCCTGGA
    GAAGATGGAG ACAAGGGAGA GATTGGGGAG CCAGGACAGA AAGGGAGCAA GGGAGACAAA
    GGCGAGCAGG GTCCACCAGG GCCTACAGGA CCTCAAGGCC CCATCGGACA GCCAGGCCCC
    TCCGGAGCCG ATGGCGAGCC GGGACCTCGG GGCCAGCAGG GCCTCTTTGG GCAGAAAGGC
    GACGAGGGTC CGAGAGGCTT CCCCGGGCCC CCGGGCCCCG TGGGGCTGCA GGGTCTGCCG
    GGACCTCCCG GAGAGAAGGG CGAGACAGGC GACGTGGGTC AGATGGGCCC GCCAGGTCCC
    CCCGGACCCC GAGGACCCTC CGGAGCTCCA GGTGCAGACG GGCCACAAGG ACCCCCCGGC
    GGAATAGGCA ACCCTGGAGC CGTGGGAGAG AAGGGTGAAC CCGGCGAAGC AGGAGAGCCT
    GGCCTTCCCG GCGAAGGGGG CCCCCCGGTG AGTGAGCGGG CTCTGGGGAG GGGTGGGGCG
    TGGCATGGAC GGCTGCCGGG GGGCAGGGTC CCACTCTACC TCAGCTTTCC CATCCGTGAA
    ATGGGAGTCG GGCCTCCTCT TGTGCCTCCA TCACGAGACT GCTGGAAAAA AGAGGGAGTG
    TGGTGGGGAG TGAGTGTGTG TGTGACAGAG AGTGCCTGTG TGTGTGCGTG TGTGTGCGCG
    TGTGTGGAGG GTCTGCAGAG CATCGAGTCC TACAGTTCAG GGTTCAGGAT CAGGGTCAGC
    CACGGCCCCA GGAGCAGACA CCTGGGCAGG AGGCTGCCCT CTGAGGCCTC TGGAAGGCTG
    GCGGGAGGCA TGGGCCGGCT GTGTGGACGC AGCCCTGTGA GTCCAGCGCG CTCTGGGAGG
    GTGGCTGACG CTTCTCTCGC TCCATTTCAG GGCCCCGTCG GTTTTCCCGG AGATCCCGGT
    CCCCCCGGAG AACCCGGCCC CGCGGGTCAA GACGGTCCCC CTGGCGACAA AGGAGACGAT
    GGTGAACCCG GGCAAACGGG ATCACCAGGC CCTACGGGTG AGCCAGGCCC ATCTGGGCCT
    CCAGGAAAAA GGGGTCCGCC CGGCCCCGCA GGTCCCGAAG GCAGGCAGGG GGAGAAAGGA
    GCCAAGGGAG AAGCCGGCTT GGAAGGCCCT CCTGGGAAGA CTGGCCCCAT TGGCCCCCAG
    GGGGCCCCCG GGAAGCCGGG GCCCGACGGC CTTCGAGGGA TCCCGGGCCC AGTGGGTGAA
    CAAGGTCTCC CCGGAGCCCC GGGCCCTGAT GGCCCCCCAG GACCCATGGG CCCCCCAGGA
    CTCCCCGGCC TCAAAGGAGA CTCTGGACCC AAAGGAGAGA AGGGTCACCC AGGCTTGATC
    GGCCTCATCG GACCTCCGGG CGAACAGGGA GAAAAGGGTG ACCGCGGAGT CCCCGGCCCC
    CAGGGCTCCT CGGGCCCTAA GGGAGAACAG GGTATCACCG GTCCTTCCGG CCCGATTGGC
    CCCCCGGGTC CCCCCGGCCT GCCGGGGCCC CCTGGTCCAA AAGGCGCTAA GGGCTCCTCG
    GGTCCCACTG GCCCGAAGGG TGAGGCAGGC CAGCCAGGAC CCCCTGGCCC ACCAGGCCCC
    CCAGGCGAGG TCATCCAGCC CCTGCCGATC CAGGCATCCA GGACCCGGCG GAATATCGAT
    GCCAGCCAGC TGATGGACGA CGCAGAGGGC GAGCAGTACA TGGACTATGC GGATGGCATG
    GAGGAGATCT TCGGCTCGCT CAACTCCCTG AAGCTGGAGA TCGAGCAGAT GAAGCGGCCG
    CTGGGCACAC CGCAGAACCC CGCACGCACC TGCAAAGACC TCCAGCTCTG CCACCCCGAC
    TTCCCTGACG GCGAATACTG GGTGGATCCG AACCAAGGCT GCTCCCGGGA CTCCTTCAAG
    GTCTACTGCA ACTTCACGGC CGGGGGGTCA ACGTGCGTCT TCCCCGACAA GAAGTCTGAA
    GGGGCCAGAA TCACTTCTTG GCCCAAAGAA AACCCGGGTT CCTGGTTCAG TGAATTCAAG
    CGTGGCAAAC TGCTCTCCTA TGTGGACGCC GAGGGCAACC CCGTGGGCGT CGTCCAGATG
    ACCTTCCTGC GGCTGCTCAG CGCCTCCGCC CACCAGAACA TCACCTACAA CTGCTACCAG
    TCGGTGGCCT GGCAGGACGC CGCCACGGGC AGCTATGACA AGGCCATGCG CTTCCTGGGC
    TCCAACGACG AGGAGATGTC ATATGACAAC AGCCCCTACA TCCGCGCCCT GGTGGACGGC
    TGCGCCACGA AGAAAGGGTA TCAGAAGACG GTTCTGGAGA TCGACACGCC CAAGGTGGAG
    CAGGTGCCCA TCGTCGACAT CATGTTCAAC GACTTCGGTG AAGCGTCACA GAAATTTGGA
    TTTGAAGTGG GGCCGGCTTG CTTCCTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329224.1
    CDS417..4949
    Translation

    Target ORF information:

    RefSeq Version XM_015473738.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type V alpha 1 (COL5A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCTGCCA ACCAGGACAC CATCTATGAG GGGATGGGAG GGCCTCGGGG CGAGAAAGGT 
    CAAAAAGGAG AACCCGCCAT CATCGAACCG GGGATGCTCC TGGAGGGGCC GCCTGGCCCC
    GAAGGCCCCG CGGGTCTCCC AGGACCTCCA GGAACGATGG GTCCCACAGG CCAAGTGGGT
    GACCCTGGAG AAAGAGGTCC CCCTGGACGC CCAGGCCTTC CTGGTGCTGA CGGGCTGCCC
    GGCCCTCCTG GAACCATGCT TATGCTGCCT TTCCGGTTCG GAGGGGGCGG CGACGCGGGC
    TCCAAAGGAC CCATGGTCTC CGCGCAGGAG TCCCAAGCTC AAGCCATCCT GCAACAGGCC
    CGGCTGGCGT TGAGGGGGCC TGCTGGCCCA ATGGGTCTCA CTGGGAGACC GGGTCCCATG
    GGCCCTCCTG GGAGCGGAGG TCTGAAGGGT GAGCCAGGAG ACATGGGGCC TCAGGGTCCG
    CGGGGTGTGC AGGGCCCGCC TGGCCCGGCA GGAAAGCCTG GGAGAAGGGG CCGAGCCGGC
    AGTGATGGAG CGAGAGGGAT GCCTGGGCAG ACTGGCCCCA AGGGTGACCG CGGCTTCGAT
    GGCCTGGCGG GGCTGCCGGG GGAGAAGGGC CACCGGGGCG ACCCTGGCCC GTCCGGCCCA
    CCGGGACCTC CGGGGGAAGA CGGAGAAAGG GGCGACGATG GGGAAGTCGG GCCCAGAGGG
    CTGCCTGGGG AGCCCGGGCC GCGAGGTCTG CTGGGGCCGA AGGGGCCCCC AGGCCCCCCT
    GGACCGCCCG GTGTGACGGG AATGGATGGT CAACCAGGCC CAAAAGGCAA CGTGGGTCCC
    CAGGGAGAGC CCGGCCCCCC GGGGCAGCAG GGCAACCCAG GTGCCCAGGG TCTTCCGGGC
    CCCCAGGGCG CTATCGGGCC TCCAGGAGAA AAGGGTCCCC TGGGCAAACC CGGCCTCCCA
    GGAATGCCTG GTGCTGACGG ACCCCCGGGG CACCCTGGCA AAGAAGGCCC TCCAGGAGAG
    AAGGGAGGGC AGGGTCCTCC CGGCCCCCAG GGTCCAATCG GCTACCCAGG TCCTCGCGGA
    GTCAAGGGGG CAGACGGCAT CCGAGGTCTG AAGGGCACCA AGGGTGAGAA GGGTGAAGAT
    GGCTTCCCAG GCTTCAAAGG AGACATGGGC ATCAAGGGCG ATCGGGGGGA GATCGGCCCA
    CCTGGTCCAN GAGGGGAGGA CGGTCCTGAA GGTCCCAAGG GGCGAGGCGG TCCGAACGGC
    GACCCCGGTC CTCTGGGACC CCCTGGGGAG AAGGGCAAAC TCGGAGTCCC AGGACTGCCA
    GGCTACCCAG GAAGACAGGG GCCAAAGGGT TCTATCGGAT TTCCTGGATT TCCCGGCGCC
    AACGGAGAAA AGGGCGGCAG GGGCACCCCT GGGAAGCCGG GACCACGGGG GCAGCGAGGC
    CCGACGGGCC CACGAGGTGA AAGAGGCCCA CGGGGCATCA CCGGCAAGCC TGGCCCCAAG
    GGCAACTCTG GAGGCGATGG CCCGGCCGGC CCTCCAGGTG AACGGGGACC CAACGGACCC
    CAAGGACCCA CGGGGTTCCC TGGACCAAAG GGCCCCCCGG GGCCCCCAGG CAAGGACGGG
    CTCCCGGGGC ACCCTGGACA GCGAGGCGAG ACGGGTTTCC AAGGCAAGAC CGGCCCTCCA
    GGCCCCCCAG GTGTGGTCGG CCCTCAGGGT CCCACCGGGG AAACTGGTCC CATGGGCGAG
    CGTGGCCACC CTGGGCCCCC AGGCCCCCCC GGTGAACAGG GGCTCCCGGG CCTGGCCGGG
    AAAGAAGGGA CGAAGGGTGA CCCAGGCCCC GCCGGCCTTC CTGGGAAAGA CGGTCCCCCC
    GGGCTGCGAG GCTTCCCTGG AGACCGAGGC CTTCCTGGTC CAGTGGGGGC GCTTGGACTG
    AAAGGCAGTG AAGGCCCCCC CGGCCCACCA GGCCCCGCGG GATCTCCAGG CGAGAGAGGT
    CCAGCCGGTG CTGCTGGGCC CATTGGAATT CCAGGGAGAC CCGGGCCCCA GGGGCCCCCT
    GGGCCAGCTG GAGAGAAAGG CGCTCCTGGC GAGAAGGGTC CACAAGGCCC AGCTGGCCGA
    GACGGTCTCC AGGGCCCCGT GGGGCTCCCC GGTCCCGCTG GCCCTGTGGG CCCCCCTGGA
    GAAGATGGAG ACAAGGGAGA GATTGGGGAG CCAGGACAGA AAGGGAGCAA GGGAGACAAA
    GGCGAGCAGG GTCCACCAGG GCCTACAGGA CCTCAAGGCC CCATCGGACA GCCAGGCCCC
    TCCGGAGCCG ATGGCGAGCC GGGACCTCGG GGCCAGCAGG GCCTCTTTGG GCAGAAAGGC
    GACGAGGGTC CGAGAGGCTT CCCCGGGCCC CCGGGCCCCG TGGGGCTGCA GGGTCTGCCG
    GGACCTCCCG GAGAGAAGGG CGAGACAGGC GACGTGGGTC AGATGGGCCC GCCAGGTCCC
    CCCGGACCCC GAGGACCCTC CGGAGCTCCA GGTGCAGACG GGCCACAAGG ACCCCCCGGC
    GGAATAGGCA ACCCTGGAGC CGTGGGAGAG AAGGGTGAAC CCGGCGAAGC AGGAGAGCCT
    GGCCTTCCCG GCGAAGGGGG CCCCCCGGTG AGTGAGCGGG CTCTGGGGAG GGGTGGGGCG
    TGGCATGGAC GGCTGCCGGG GGGCAGGGTC CCACTCTACC TCAGCTTTCC CATCCGTGAA
    ATGGGAGTCG GGCCTCCTCT TGTGCCTCCA TCACGAGACT GCTGGAAAAA AGAGGGAGTG
    TGGTGGGGAG TGAGTGTGTG TGTGACAGAG AGTGCCTGTG TGTGTGCGTG TGTGTGCGCG
    TGTGTGGAGG GTCTGCAGAG CATCGAGTCC TACAGTTCAG GGTTCAGGAT CAGGGTCAGC
    CACGGCCCCA GGAGCAGACA CCTGGGCAGG AGGCTGCCCT CTGAGGCCTC TGGAAGGCTG
    GCGGGAGGCA TGGGCCGGCT GTGTGGACGC AGCCCTGTGA GTCCAGCGCG CTCTGGGAGG
    GTGGCTGACG CTTCTCTCGC TCCATTTCAG GGCCCCGTCG GTTTTCCCGG AGATCCCGGT
    CCCCCCGGAG AACCCGGCCC CGCGGGTCAA GACGGTCCCC CTGGCGACAA AGGAGACGAT
    GGTGAACCCG GGCAAACGGG ATCACCAGGC CCTACGGGTG AGCCAGGCCC ATCTGGGCCT
    CCAGGAAAAA GGGGTCCGCC CGGCCCCGCA GGTCCCGAAG GCAGGCAGGG GGAGAAAGGA
    GCCAAGGGAG AAGCCGGCTT GGAAGGCCCT CCTGGGAAGA CTGGCCCCAT TGGCCCCCAG
    GGGGCCCCCG GGAAGCCGGG GCCCGACGGC CTTCGAGGGA TCCCGGGCCC AGTGGGTGAA
    CAAGGTCTCC CCGGAGCCCC GGGCCCTGAT GGCCCCCCAG GACCCATGGG CCCCCCAGGA
    CTCCCCGGCC TCAAAGGAGA CTCTGGACCC AAAGGAGAGA AGGGTCACCC AGGCTTGATC
    GGCCTCATCG GACCTCCGGG CGAACAGGGA GAAAAGGGTG ACCGCGGAGT CCCCGGCCCC
    CAGGGCTCCT CGGGCCCTAA GGGAGAACAG GGTATCACCG GTCCTTCCGG CCCGATTGGC
    CCCCCGGGTC CCCCCGGCCT GCCGGGGCCC CCTGGTCCAA AAGGCGCTAA GGGCTCCTCG
    GGTCCCACTG GCCCGAAGGG TGAGGCAGGC CAGCCAGGAC CCCCTGGCCC ACCAGGCCCC
    CCAGGCGAGG TCATCCAGCC CCTGCCGATC CAGGCATCCA GGACCCGGCG GAATATCGAT
    GCCAGCCAGC TGATGGACGA CGCAGAGGGC GAGCAGTACA TGGACTATGC GGATGGCATG
    GAGGAGATCT TCGGCTCGCT CAACTCCCTG AAGCTGGAGA TCGAGCAGAT GAAGCGGCCG
    CTGGGCACAC CGCAGAACCC CGCACGCACC TGCAAAGACC TCCAGCTCTG CCACCCCGAC
    TTCCCTGACG GCGAATACTG GGTGGATCCG AACCAAGGCT GCTCCCGGGA CTCCTTCAAG
    GTCTACTGCA ACTTCACGGC CGGGGGGTCA ACGTGCGTCT TCCCCGACAA GAAGTCTGAA
    GGGGCCAGAA TCACTTCTTG GCCCAAAGAA AACCCGGGTT CCTGGTTCAG TGAATTCAAG
    CGTGGCAAAC TGCTCTCCTA TGTGGACGCC GAGGGCAACC CCGTGGGCGT CGTCCAGATG
    ACCTTCCTGC GGCTGCTCAG CGCCTCCGCC CACCAGAACA TCACCTACAA CTGCTACCAG
    TCGGTGGCCT GGCAGGACGC CGCCACGGGC AGCTATGACA AGGCCATGCG CTTCCTGGGC
    TCCAACGACG AGGAGATGTC ATATGACAAC AGCCCCTACA TCCGCGCCCT GGTGGACGGC
    TGCGCCACGA AGAAAGGGTA TCAGAAGACG GTTCTGGAGA TCGACACGCC CAAGGTGGAG
    CAGGTGCCCA TCGTCGACAT CATGTTCAAC GACTTCGGTG AAGCGTCACA GAAATTTGGA
    TTTGAAGTGG GGCCGGCTTG CTTCCTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.