PHRF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHRF1
Entrez Gene ID	100126170
Full Name	PHD and ring finger domains 1
General protein information	Preferred Names PHD and ring finger domains 1 Names PHD and RING finger domain-containing protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005227257.4	XP_005227314.1	PHD and RING finger domain-containing protein 1 isoform X2
NM_001109963.2	NP_001103433.2	PHD and RING finger domain-containing protein 1
XM_005227256.4	XP_005227313.1	PHD and RING finger domain-containing protein 1 isoform X1
XM_005227256.3	XP_005227313.1	PHD and RING finger domain-containing protein 1 isoform X1
XM_005227257.3	XP_005227314.1	PHD and RING finger domain-containing protein 1 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PHRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40766	NM_001109963.2 Latest version!	Bos taurus PHD and ring finger domains 1 (PHRF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa34951	XM_005227256.4 Latest version!	Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa34952	XM_005227257.4 Latest version!	Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OBa34951	XM_005227256.3	Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa34952	XM_005227257.3	Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHRF1 ( NM_001109963.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001103433.2 Bos taurus PHD and ring finger domains 1 (PHRF1), mRNA.

Price & Availability↑

CloneID	OBa40766
Clone ID Related Accession (Same CDS sequence)	NM_001109963.2
Accession Version	NM_001109963.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	PHD and RING finger domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC149467.1 and BM483181.1. On Nov 14, 2011 this sequence version replaced NM_001109963.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGATGACG ACAGCCTGGA CGAGCTTGTG GACCGAAGCC CGGGGCCCGA TGGCCACCCC 
AGACTCCGTC CTGCCGCCCT GGCCAGCGAT GCTGGAAGCA GTGATGCCCA CAGCGACGCC 
TCTGAGGACG ACTCTGGCAG CGAGCACAGC GATGAGGAGG ATGCGGACGG GGACCTGGAG 
GACGGATCTG GTTCCGAGGA CTCTGAGGAC GATGGTGACG ACCTGGACGC GTTGGCAGCG 
GCGGCGGATT CTCAGGGAGC TCTGGAAGGC AGCGGTGCGT TCAATTCTGA TGACGACTCC 
GAGAGCTGCC CGATCTGTCT CAATGCTTTC AGGGAGCAGG CTGTGGGAAC GCCCGAGAAC 
TGCGCCCACT ACTTCTGCCT CGATTGCATT GTGGAGTGGT CCAAGAATGC CAATTCCTGT 
CCAGTTGATC GAACTATATT TAAGTGTATT TGTATTCGAG CTCAATTTGG TGGTAAAATC 
TTGAAGAAGA TTCCAGTAGA GAGTGCCCAC CTTGGGGAGA ACGAGAATGA AGACCCGACC 
TTCTGTGAGG TGTGTGGCCG GAGCGACCGG GAGGACCGGC TGCTGCTCTG TGACGGCTGC 
GACGCTGGGT ACCACATGGA GTGCTTGGAC CCCCCTCTCC AGGAGGTTCC AGTGGATGAG 
TGGTTCTGTC CGGAATGTGC TACCCCCGGG GCTGCTTCTG CCGCTGATGC GGGTCCTGTG 
AGCGAGGAAG AGGTCTCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCCCGCACGG GGAGGACACG GGCCATCGCC AGGACGAGGC AGAGCGAGAG GGTGCGAGCG 
ACAGTGAACC GGAACCGGAT CTCCACGGCC AGGAGGTTCC AGCGTGTCCC CAGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATTGAA GCCGTGGCTG CCGGGCTCAG CACGGCTGTG 
TACCAGCGCC CCGGGCCGCG TGCCCCAGCC CGCCGCAGGA GGAAAGGAGG AAGACGGAAG 
AAAACGTCAG GAAGAAAGAA AGCCCCGTCC AGATTGTCTG CAAAGAGCAG GAGCTCAGGG 
GCAAGGTTGA AGAAACGCCC AGGTCGTGTG AAGAAGAGGA AAGCACAGAA GATAAAGAAT 
GAAGCCACGG CTCGTTCGCG CATCGCACGG ACGCTGGGCC TCTGCAGGCC GGCCTGTGGA 
GCCTGCACCC CCTCCGTGTA CAAGCCTGCA GACCCGTCTC TGGGGCTGAT GCGAGCCGAC 
ATCGGGGCAG CCTCCCTCTC CCTGTTTGGA GACCCCTATG AATTGGATCC CTTTGACAGT 
GAAGAGGCGT CTGCAAGTCC TGCTTCCCCT CTGAGCGCCA AGAGAAGGGT TCTTTCCCGA 
TCAGCCCTGC GCTCCCACCA GCCTGTAGCC AGACCCATCT CCATGGGGCT CTCCAGGAGA 
GGTGCTCCTG CCGTGGCCCC CCAGCCGGAA GTGGACACAG AGCCCAGCCC TGACCTGCTG 
GGGAGCATCC TGTCTGGCCA GAGCCTTCTG ATGATGAACG GTGCCGACAT CATCATCCAC 
CGCGACGGCT CCCTCAGCGC CAAGAGGGCA GTGCCAGTCT CTTTTCAGCA GAACTCAGCT 
GGTCCATCCA GAGAGCCAGA AGGCGCCTGG GGGCGTCAGT CAGGAAGCGG GCCACAGAGC 
TCGGGGCCCA GCTGCCCGGG CAGGGCCGCC CCCGCCCCCA CCCGTGTCCC TGCACTCACA 
TCGGGGGCAG CGGCAAAGCC GGACTCCTCG GTGACCCCTC GGTTGGGCCA GTCTCAGACC 
TCGTCCAGCG AGAGCAGACC TGGTATACAG CAGAGTGAGG GTGGCCAGTG TAACGGTGAC 
AGCAAGCACT CTCTGCCTCT TGGGCCTGCG GCGCTGAAGG CCTCACACAG CAGTTCTGAC 
TCGCCCTCCA AGAGTACAGT GCCGGGCCAG GCCCCGAGAC CAGCTCCCAG GCGGACAGAC 
ATCTCCGAGC TCCCCAGGAT ACCAAAGATC AGGAGAGACA GCAGCCGGGG GGACGAGGGC 
CCTGCCAGTA GGCAGGGCGT CGAGCTCCCC AGCTCCTGCA TCAGCCGGCT GACAGGCAGG 
GAGGGCCCTG GACAGCCTGG CTGCGGGGCC CGGGTGGAGG GTGAGCCCAG CAACCGGGGC 
GCACAGGAGC CCAGCACGCA CTCCGGGGGG GGCCCCCAGC CCCCCGGCCC GCTGGGGCCG 
GCCCGGGGGA AGGCTGTTGG CTCCACCTTT GAGAGTTTCA GGATCAACAT TCCTGGGAAC 
ACAGCCCATT CTGGCAGACT GCTCAGCCCT GGCTTCTGTA ACACGTTCCG GCCGGTGGAC 
AGCAAGGTGC AGAGGAGAGC GAGCCCCTCG CCCCTCTTCT CTGTCAGGAA GACGAAGCAG 
CTCAAGAGCG AGATCTATGA CCCCTTCAAC CCCACGGGCT CCGACTCCAG CTCTGCCAAC 
AGCAGCCCCG AGCGTCTGGG CCTCCTGCCC TCGGAGATCA CGCGGACCAT CTCTGTTGAC 
AGCCCCAAGG CCCCGGTGCT GCCCCCCGTG CGCTGCGTCA CCTCCTACAC GGTGCAGACA 
GGGGCGGGGA GAGAGCTGGA GCCCCCCCAG GGGCCCTCCA GTCTGCCTGA GCTCCAGGAT 
GAGGAGCCCT GCGCACAGGC TCAGCGGCCG TCCCCCCCGG AGCCCTGGGG CGATGAGGAG 
CTGCCTCCCC ACAGCTCCTT CTTCGGCTCC GAGGGCCGGA CAGTGACCTG CGTGACTGTG 
GTGGAGCCCG ACACGCCGCC CAGCCCAGAC GCCCCGCCTG CCACCACCCA CAGGGTCGTG 
GAGCTGCGCT CCCTCTCCCG CTCCCGCTCC CGCTCCAGTT CCCGCAGCCG GAAGAAAGGC 
CCGCAGCCGA GGGCGGCCAC CAGGGAGCAC CGTGCCGGCC GCTCAGGCTC CCGCTCTTCC 
CGCTCAGGAG ACCGGAGTTC CAGGTCTGCC TCGCCGCCGG CGGGCGAGGA CCAGCCCAAG 
GGACACCGGC CCAAGGCCCG GGGCCGCAGG TCCTCCAGCG AGCACTCCAG CAGCCGCGAG 
CGTGCCAAGC GGAAGAAGGC GAAGGACAAG AGCAGGGAGC GCGGCAGAGG CTCCTGGGGT 
CGCGGCCGTC GGAGGTCCCG CTCCCGCTCT GGGAGCCCTG GGAGCTCGTC CCACGAGCAC 
CGCGAGGGCC GCAGGAAGAA GAGGCGACGG TCAGGGTCCA GGTCTCGGGG GAGGGAGTGC 
TCGCCCCCTA GGCACCCCAG GGAGAGGAGG GGGCGGCCCC GAGACAGGCG CGGCTCCCGC 
GAGAGGCGGC GGCGAAGGTC CAGGTCCCCC AGCTCGGAAC ACCGGCCCCG GGCGCACCGG 
CGGCCTCGGT CCCGGGAGAA GCGCTCGCGG CCCCGCCCCC ACTCCCCTGA GCGGAAGTTG 
GCCCCGAAAG AGGCTTCTGC AGCACCCGCT CCCCAGGGGG AGCCCAGGCC AGACAGGGAG 
CCCCCAGCCA GGTCCCCGGC TAGACCCCCG TCCTCAGCGG GAGCAGACGG CTTGCCAGAA 
GGGCCCGAAG TCCACAAGAC TGCCCCGGTC CCGGAGGTGC TGGCCGCGTG TCCAGCGGAG 
GACGTGGACT ATGGCGACTC TGTGGAGGCG GGGCACCTCT TTGAGGACTT TTCCAGTGAC 
ACCATCTTCA TGCAGCTTGA TGATATGAGT TCCCCGCCCT CCCCGGAGAG CACGGACTCC 
TCCCCAGAGC GTGACCTCCT GCCCAAGACC ACCATGCCCC CAGCTGGCCC CCAGCACGAC 
ACCAGCCTGG CCATGGCCAT CATCCGGAGG GAGGTGTCTC GGATCCACGG TGAAGATGTG 
GCACAGCCCT CGCCTGGGAC CGAGGGCCCC CAGGAGAAGC ACTTGCTCCA GCAGGACGCA 
GACGAGGCCG CCGCAGTGCC CTGTGCCCTG GGAGCCCAGG CCCCGGGCGG GATGCCTGTG 
GGGAAGGAGG AAGGCCCCTC TCAGAACCCC CTGCTGCGGG CTAAGGCGCT GGTGAAGAGG 
GTCACCTGGA ACCTCCAGGA GGCAGAGTGC GGGGCCCCGG CCAATGACAG AGGCCTGTCC 
AGGAGGCAGT CTTGTGGCCT AGAGGGCCCC AGAACACCCA CTCCCATATG TTGGCCCCCA 
GGGACACCAC TTCACAGGCC ACAAAAGCCC CGGGAAGGAG TCTGGGAATC GGACGACGTG 
GGCCCCATGG CTGGGTTCCA GCAGGCACCC TTCTCTGAGC CGCCGGCCCC TGCTTATGCG 
CTTCCAGAGC CTGGCTTCCC CTACACTGAC CCCTCTCAGG TACAGAGCCC CAGCCTGCCC 
CCTGGCCCAG CCCTGCCATC GGGCATCCCG CCTTATGCAC CTATCAGCCA GCCCGCGGTC 
CAGTTCCTCC TCCAGGGGAG CCTGCCCCTC CCGGGCTGCG GGGCGGCCCA GAGCATGGCC 
CCAGTGTCCA CCACCCCGAC TGCAGCCTCA GAGCCAGCTG GCCACGCTGC CGCCACCACT 
GCTGCCAACA ACTCTGAGGA GAGGACGGCT CCCCCCAGGC CGACCCCAGA GAAGGCCAAG 
AATGAGGAGT ACATGAAGAA GCTGCACGTG CAGGAGCGGG CCGTGGAGGA GGTGAAGCTG 
GCCATCAAGC CCTTCTACCA GAAGAGGGAG GTGACCAAGG AGGAGTATAA GGACATCCTG 
CGCAAGGCGG TGCAGAAGAT TTGCCACAGC AAGAGCGGGG AGATCAACCC CGTGAAGGTG 
GGCAACCTGG TGAAGGCCTA CGTGGACAAG TACCGGCACA TGCGCAGACA CCGGCGGGCC 
GAGGCCGGCG AGGAGCCGCC CGCCCAGAGC GCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103433.2
CDS	143..4984
Translation	MDDDSLDELVDRSPGPDGHPRLRPAALASDAGSSDAHSDASEDDSGSEHSDEEDADGDLEDGSGSEDSEDDGDDLDALAAAADSQGALEGSGAFNSDDDSESCPICLNAFREQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRAQFGGKILKKIPVESAHLGENENEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECATPGAASAADAGPVSEEEVSLLLADVVPTTSRLRPRTGRTRAIARTRQSERVRATVNRNRISTARRFQRVPRYLMSSLLDETIEAVAAGLSTAVYQRPGPRAPARRRRKGGRRKKTSGRKKAPSRLSAKSRSSGARLKKRPGRVKKRKAQKIKNEATARSRIARTLGLCRPACGACTPSVYKPADPSLGLMRADIGAASLSLFGDPYELDPFDSEEASASPASPLSAKRRVLSRSALRSHQPVARPISMGLSRRGAPAVAPQPEVDTEPSPDLLGSILSGQSLLMMNGADIIIHRDGSLSAKRAVPVSFQQNSAGPSREPEGAWGRQSGSGPQSSGPSCPGRAAPAPTRVPALTSGAAAKPDSSVTPRLGQSQTSSSESRPGIQQSEGGQCNGDSKHSLPLGPAALKASHSSSDSPSKSTVPGQAPRPAPRRTDISELPRIPKIRRDSSRGDEGPASRQGVELPSSCISRLTGREGPGQPGCGARVEGEPSNRGAQEPSTHSGGGPQPPGPLGPARGKAVGSTFESFRINIPGNTAHSGRLLSPGFCNTFRPVDSKVQRRASPSPLFSVRKTKQLKSEIYDPFNPTGSDSSSANSSPERLGLLPSEITRTISVDSPKAPVLPPVRCVTSYTVQTGAGRELEPPQGPSSLPELQDEEPCAQAQRPSPPEPWGDEELPPHSSFFGSEGRTVTCVTVVEPDTPPSPDAPPATTHRVVELRSLSRSRSRSSSRSRKKGPQPRAATREHRAGRSGSRSSRSGDRSSRSASPPAGEDQPKGHRPKARGRRSSSEHSSSRERAKRKKAKDKSRERGRGSWGRGRRRSRSRSGSPGSSSHEHREGRRKKRRRSGSRSRGRECSPPRHPRERRGRPRDRRGSRERRRRRSRSPSSEHRPRAHRRPRSREKRSRPRPHSPERKLAPKEASAAPAPQGEPRPDREPPARSPARPPSSAGADGLPEGPEVHKTAPVPEVLAACPAEDVDYGDSVEAGHLFEDFSSDTIFMQLDDMSSPPSPESTDSSPERDLLPKTTMPPAGPQHDTSLAMAIIRREVSRIHGEDVAQPSPGTEGPQEKHLLQQDADEAAAVPCALGAQAPGGMPVGKEEGPSQNPLLRAKALVKRVTWNLQEAECGAPANDRGLSRRQSCGLEGPRTPTPICWPPGTPLHRPQKPREGVWESDDVGPMAGFQQAPFSEPPAPAYALPEPGFPYTDPSQVQSPSLPPGPALPSGIPPYAPISQPAVQFLLQGSLPLPGCGAAQSMAPVSTTPTAASEPAGHAAATTAANNSEERTAPPRPTPEKAKNEEYMKKLHVQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEPPAQSAEG

Target ORF information:

RefSeq Version	NM_001109963.2
Organism	Bos taurus(cattle)
Definition	Bos taurus PHD and ring finger domains 1 (PHRF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109963.2

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA CGAGCTTGTG GACCGAAGCC CGGGGCCCGA TGGCCACCCC 
AGACTCCGTC CTGCCGCCCT GGCCAGCGAT GCTGGAAGCA GTGATGCCCA CAGCGACGCC 
TCTGAGGACG ACTCTGGCAG CGAGCACAGC GATGAGGAGG ATGCGGACGG GGACCTGGAG 
GACGGATCTG GTTCCGAGGA CTCTGAGGAC GATGGTGACG ACCTGGACGC GTTGGCAGCG 
GCGGCGGATT CTCAGGGAGC TCTGGAAGGC AGCGGTGCGT TCAATTCTGA TGACGACTCC 
GAGAGCTGCC CGATCTGTCT CAATGCTTTC AGGGAGCAGG CTGTGGGAAC GCCCGAGAAC 
TGCGCCCACT ACTTCTGCCT CGATTGCATT GTGGAGTGGT CCAAGAATGC CAATTCCTGT 
CCAGTTGATC GAACTATATT TAAGTGTATT TGTATTCGAG CTCAATTTGG TGGTAAAATC 
TTGAAGAAGA TTCCAGTAGA GAGTGCCCAC CTTGGGGAGA ACGAGAATGA AGACCCGACC 
TTCTGTGAGG TGTGTGGCCG GAGCGACCGG GAGGACCGGC TGCTGCTCTG TGACGGCTGC 
GACGCTGGGT ACCACATGGA GTGCTTGGAC CCCCCTCTCC AGGAGGTTCC AGTGGATGAG 
TGGTTCTGTC CGGAATGTGC TACCCCCGGG GCTGCTTCTG CCGCTGATGC GGGTCCTGTG 
AGCGAGGAAG AGGTCTCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCCCGCACGG GGAGGACACG GGCCATCGCC AGGACGAGGC AGAGCGAGAG GGTGCGAGCG 
ACAGTGAACC GGAACCGGAT CTCCACGGCC AGGAGGTTCC AGCGTGTCCC CAGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATTGAA GCCGTGGCTG CCGGGCTCAG CACGGCTGTG 
TACCAGCGCC CCGGGCCGCG TGCCCCAGCC CGCCGCAGGA GGAAAGGAGG AAGACGGAAG 
AAAACGTCAG GAAGAAAGAA AGCCCCGTCC AGATTGTCTG CAAAGAGCAG GAGCTCAGGG 
GCAAGGTTGA AGAAACGCCC AGGTCGTGTG AAGAAGAGGA AAGCACAGAA GATAAAGAAT 
GAAGCCACGG CTCGTTCGCG CATCGCACGG ACGCTGGGCC TCTGCAGGCC GGCCTGTGGA 
GCCTGCACCC CCTCCGTGTA CAAGCCTGCA GACCCGTCTC TGGGGCTGAT GCGAGCCGAC 
ATCGGGGCAG CCTCCCTCTC CCTGTTTGGA GACCCCTATG AATTGGATCC CTTTGACAGT 
GAAGAGGCGT CTGCAAGTCC TGCTTCCCCT CTGAGCGCCA AGAGAAGGGT TCTTTCCCGA 
TCAGCCCTGC GCTCCCACCA GCCTGTAGCC AGACCCATCT CCATGGGGCT CTCCAGGAGA 
GGTGCTCCTG CCGTGGCCCC CCAGCCGGAA GTGGACACAG AGCCCAGCCC TGACCTGCTG 
GGGAGCATCC TGTCTGGCCA GAGCCTTCTG ATGATGAACG GTGCCGACAT CATCATCCAC 
CGCGACGGCT CCCTCAGCGC CAAGAGGGCA GTGCCAGTCT CTTTTCAGCA GAACTCAGCT 
GGTCCATCCA GAGAGCCAGA AGGCGCCTGG GGGCGTCAGT CAGGAAGCGG GCCACAGAGC 
TCGGGGCCCA GCTGCCCGGG CAGGGCCGCC CCCGCCCCCA CCCGTGTCCC TGCACTCACA 
TCGGGGGCAG CGGCAAAGCC GGACTCCTCG GTGACCCCTC GGTTGGGCCA GTCTCAGACC 
TCGTCCAGCG AGAGCAGACC TGGTATACAG CAGAGTGAGG GTGGCCAGTG TAACGGTGAC 
AGCAAGCACT CTCTGCCTCT TGGGCCTGCG GCGCTGAAGG CCTCACACAG CAGTTCTGAC 
TCGCCCTCCA AGAGTACAGT GCCGGGCCAG GCCCCGAGAC CAGCTCCCAG GCGGACAGAC 
ATCTCCGAGC TCCCCAGGAT ACCAAAGATC AGGAGAGACA GCAGCCGGGG GGACGAGGGC 
CCTGCCAGTA GGCAGGGCGT CGAGCTCCCC AGCTCCTGCA TCAGCCGGCT GACAGGCAGG 
GAGGGCCCTG GACAGCCTGG CTGCGGGGCC CGGGTGGAGG GTGAGCCCAG CAACCGGGGC 
GCACAGGAGC CCAGCACGCA CTCCGGGGGG GGCCCCCAGC CCCCCGGCCC GCTGGGGCCG 
GCCCGGGGGA AGGCTGTTGG CTCCACCTTT GAGAGTTTCA GGATCAACAT TCCTGGGAAC 
ACAGCCCATT CTGGCAGACT GCTCAGCCCT GGCTTCTGTA ACACGTTCCG GCCGGTGGAC 
AGCAAGGTGC AGAGGAGAGC GAGCCCCTCG CCCCTCTTCT CTGTCAGGAA GACGAAGCAG 
CTCAAGAGCG AGATCTATGA CCCCTTCAAC CCCACGGGCT CCGACTCCAG CTCTGCCAAC 
AGCAGCCCCG AGCGTCTGGG CCTCCTGCCC TCGGAGATCA CGCGGACCAT CTCTGTTGAC 
AGCCCCAAGG CCCCGGTGCT GCCCCCCGTG CGCTGCGTCA CCTCCTACAC GGTGCAGACA 
GGGGCGGGGA GAGAGCTGGA GCCCCCCCAG GGGCCCTCCA GTCTGCCTGA GCTCCAGGAT 
GAGGAGCCCT GCGCACAGGC TCAGCGGCCG TCCCCCCCGG AGCCCTGGGG CGATGAGGAG 
CTGCCTCCCC ACAGCTCCTT CTTCGGCTCC GAGGGCCGGA CAGTGACCTG CGTGACTGTG 
GTGGAGCCCG ACACGCCGCC CAGCCCAGAC GCCCCGCCTG CCACCACCCA CAGGGTCGTG 
GAGCTGCGCT CCCTCTCCCG CTCCCGCTCC CGCTCCAGTT CCCGCAGCCG GAAGAAAGGC 
CCGCAGCCGA GGGCGGCCAC CAGGGAGCAC CGTGCCGGCC GCTCAGGCTC CCGCTCTTCC 
CGCTCAGGAG ACCGGAGTTC CAGGTCTGCC TCGCCGCCGG CGGGCGAGGA CCAGCCCAAG 
GGACACCGGC CCAAGGCCCG GGGCCGCAGG TCCTCCAGCG AGCACTCCAG CAGCCGCGAG 
CGTGCCAAGC GGAAGAAGGC GAAGGACAAG AGCAGGGAGC GCGGCAGAGG CTCCTGGGGT 
CGCGGCCGTC GGAGGTCCCG CTCCCGCTCT GGGAGCCCTG GGAGCTCGTC CCACGAGCAC 
CGCGAGGGCC GCAGGAAGAA GAGGCGACGG TCAGGGTCCA GGTCTCGGGG GAGGGAGTGC 
TCGCCCCCTA GGCACCCCAG GGAGAGGAGG GGGCGGCCCC GAGACAGGCG CGGCTCCCGC 
GAGAGGCGGC GGCGAAGGTC CAGGTCCCCC AGCTCGGAAC ACCGGCCCCG GGCGCACCGG 
CGGCCTCGGT CCCGGGAGAA GCGCTCGCGG CCCCGCCCCC ACTCCCCTGA GCGGAAGTTG 
GCCCCGAAAG AGGCTTCTGC AGCACCCGCT CCCCAGGGGG AGCCCAGGCC AGACAGGGAG 
CCCCCAGCCA GGTCCCCGGC TAGACCCCCG TCCTCAGCGG GAGCAGACGG CTTGCCAGAA 
GGGCCCGAAG TCCACAAGAC TGCCCCGGTC CCGGAGGTGC TGGCCGCGTG TCCAGCGGAG 
GACGTGGACT ATGGCGACTC TGTGGAGGCG GGGCACCTCT TTGAGGACTT TTCCAGTGAC 
ACCATCTTCA TGCAGCTTGA TGATATGAGT TCCCCGCCCT CCCCGGAGAG CACGGACTCC 
TCCCCAGAGC GTGACCTCCT GCCCAAGACC ACCATGCCCC CAGCTGGCCC CCAGCACGAC 
ACCAGCCTGG CCATGGCCAT CATCCGGAGG GAGGTGTCTC GGATCCACGG TGAAGATGTG 
GCACAGCCCT CGCCTGGGAC CGAGGGCCCC CAGGAGAAGC ACTTGCTCCA GCAGGACGCA 
GACGAGGCCG CCGCAGTGCC CTGTGCCCTG GGAGCCCAGG CCCCGGGCGG GATGCCTGTG 
GGGAAGGAGG AAGGCCCCTC TCAGAACCCC CTGCTGCGGG CTAAGGCGCT GGTGAAGAGG 
GTCACCTGGA ACCTCCAGGA GGCAGAGTGC GGGGCCCCGG CCAATGACAG AGGCCTGTCC 
AGGAGGCAGT CTTGTGGCCT AGAGGGCCCC AGAACACCCA CTCCCATATG TTGGCCCCCA 
GGGACACCAC TTCACAGGCC ACAAAAGCCC CGGGAAGGAG TCTGGGAATC GGACGACGTG 
GGCCCCATGG CTGGGTTCCA GCAGGCACCC TTCTCTGAGC CGCCGGCCCC TGCTTATGCG 
CTTCCAGAGC CTGGCTTCCC CTACACTGAC CCCTCTCAGG TACAGAGCCC CAGCCTGCCC 
CCTGGCCCAG CCCTGCCATC GGGCATCCCG CCTTATGCAC CTATCAGCCA GCCCGCGGTC 
CAGTTCCTCC TCCAGGGGAG CCTGCCCCTC CCGGGCTGCG GGGCGGCCCA GAGCATGGCC 
CCAGTGTCCA CCACCCCGAC TGCAGCCTCA GAGCCAGCTG GCCACGCTGC CGCCACCACT 
GCTGCCAACA ACTCTGAGGA GAGGACGGCT CCCCCCAGGC CGACCCCAGA GAAGGCCAAG 
AATGAGGAGT ACATGAAGAA GCTGCACGTG CAGGAGCGGG CCGTGGAGGA GGTGAAGCTG 
GCCATCAAGC CCTTCTACCA GAAGAGGGAG GTGACCAAGG AGGAGTATAA GGACATCCTG 
CGCAAGGCGG TGCAGAAGAT TTGCCACAGC AAGAGCGGGG AGATCAACCC CGTGAAGGTG 
GGCAACCTGG TGAAGGCCTA CGTGGACAAG TACCGGCACA TGCGCAGACA CCGGCGGGCC 
GAGGCCGGCG AGGAGCCGCC CGCCCAGAGC GCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_005227256.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227313.1 Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa34951
Clone ID Related Accession (Same CDS sequence)	XM_005227256.4 , XM_005227256.3
Accession Version	XM_005227256.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227256.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCCTGGA CGAGCTTGTG GACCGAAGCC CGGGGCCCGA TGGCCACCCC 
AGACTCCGTC CTGCCGCCCT GGCCAGCGAT GCTGAAGGAA GCAGTGATGC CCACAGCGAC 
GCCTCTGAGG ACGACTCTGG CAGCGAGCAC AGCGATGAGG AGGATGCGGA CGGGGACCTG 
GAGGACGGAT CTGGTTCCGA GGACTCTGAG GACGATGGTG ACGACCTGGA CGCGTTGGCA 
GCGGCGGCGG ATTCTCAGGG AGCTCTGGAA GGCAGCGGTG CGTTCAATTC TGATGACGAC 
TCCGAGAGCT GCCCGATCTG TCTCAATGCT TTCAGGGAGC AGGCTGTGGG AACGCCCGAG 
AACTGCGCCC ACTACTTCTG CCTCGATTGC ATTGTGGAGT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTAT ATTTAAGTGT ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTGAAGA AGATTCCAGT AGAGAGTGCC CACCTTGGGG AGAACGAGAA TGAAGACCCG 
ACCTTCTGTG AGGTGTGTGG CCGGAGCGAC CGGGAGGACC GGCTGCTGCT CTGTGACGGC 
TGCGACGCTG GGTACCACAT GGAGTGCTTG GACCCCCCTC TCCAGGAGGT TCCAGTGGAT 
GAGTGGTTCT GTCCGGAATG TGCTACCCCC GGGGCTGCTT CTGCCGCTGA TGCGGGTCCT 
GTGAGCGAGG AAGAGGTCTC CCTGCTCTTG GCTGACGTGG TGCCCACCAC CAGCCGGCTT 
CGGCCCCGCA CGGGGAGGAC ACGGGCCATC GCCAGGACGA GGCAGAGCGA GAGGGTGCGA 
GCGACAGTGA ACCGGAACCG GATCTCCACG GCCAGGAGGT TCCAGCGTGT CCCCAGGTAC 
CTCATGTCTT CTCTGCTGGA TGAGACCATT GAAGCCGTGG CTGCCGGGCT CAGCACGGCT 
GTGTACCAGC GCCCCGGGCC GCGTGCCCCA GCCCGCCGCA GGAGGAAAGG AGGAAGACGG 
AAGAAAACGT CAGGAAGAAA GAAAGCCCCG TCCAGATTGT CTGCAAAGAG CAGGAGCTCA 
GGGGCAAGGT TGAAGAAACG CCCAGGTCGT GTGAAGAAGA GGAAAGCACA GAAGATAAAG 
AATGAAGCCA CGGCTCGTTC GCGCATCGCA CGGACGCTGG GCCTCTGCAG GCCGGCCTGT 
GGAGCCTGCA CCCCCTCCGT GTACAAGCCT GCAGACCCGT CTCTGGGGCT GATGCGAGCC 
GACATCGGGG CAGCCTCCCT CTCCCTGTTT GGAGACCCCT ATGAATTGGA TCCCTTTGAC 
AGCAGTGAAG AGGCGTCTGC AAGTCCTGCT TCCCCTCTGA GCGCCAAGAG AAGGGTTCTT 
TCCCGATCAG CCCTGCGCTC CCACCAGCCT GTAGCCAGAC CCATCTCCAT GGGGCTCTCC 
AGGAGAGGTG CTCCTGCCGT GGCCCCCCAG CCGGAAGTGG ACACAGAGCC CAGCCCTGAC 
CTGCTGGGGA GCATCCTGTC TGGCCAGAGC CTTCTGATGA TGAACGGTGC CGACATCATC 
ATCCACCGCG ACGGCTCCCT CAGCGCCAAG AGGGCAGTGC CAGTCTCTTT TCAGCAGAAC 
TCAGCTGGTC CATCCAGAGA GCCAGAAGGC GCCTGGGGGC GTCAGTCAGG AAGCGGGCCA 
CAGAGCTCGG GGCCCAGCTG CCCGGGCAGG GCCGCCCCCG CCCCCACCCG TGTCCCTGCA 
CTCACATCGG GGGCAGCGGC AAAGCCGGAC TCCTCGGTGA CCCCTCGGTT GGGCCAGTCT 
CAGACCTCGT CCAGCGAGAG CAGACCTGGT ATACAGCAGA GTGAGGGTGG CCAGTGTAAC 
GGTGACAGCA AGCACTCTCT GCCTCTTGGG CCTGCGGCGC TGAAGGCCTC ACACAGCAGT 
TCTGACTCGC CCTCCAAGAG TACAGTGCCG GGCCAGGCCC CGAGACCAGC TCCCAGGCGG 
ACAGACATCT CCGAGCTCCC CAGGATACCA AAGATCAGGA GAGACAGCAG CCGGGGGGAC 
GAGGGCCCTG CCAGTAGGCA GGGCGTCGAG CTCCCCAGCT CCTGCATCAG CCGGCTGACA 
GGCAGGGAGG GCCCTGGACA GCCTGGCTGC GGGGCCCGGG TGGAGGGTGA GCCCAGCAAC 
CGGGGCGCAC AGGAGCCCAG CACGCACTCC GGGGGGGGCC CCCAGCCCCC CGGCCCGCTG 
GGGCCGGCCC GGGGGAAGGC TGTTGGCTCC ACCTTTGAGA GTTTCAGGAT CAACATTCCT 
GGGAACACAG CCCATTCTGG CAGACTGCTC AGCCCTGGCT TCTGTAACAC GTTCCGGCCG 
GTGGACAGCA AGGTGCAGAG GAGAGCGAGC CCCTCGCCCC TCTTCTCTGT CAGGAAGACG 
AAGCAGCTCA AGAGCGAGAT CTATGACCCC TTCAACCCCA CGGGCTCCGA CTCCAGCTCT 
GCCAACAGCA GCCCCGAGCG TCTGGGCCTC CTGCCCTCGG AGATCACGCG GACCATCTCT 
GTTGACAGCC CCAAGGCCCC GGTGCTGCCC CCCGTGCGCT GCGTCACCTC CTACACGGTG 
CAGACAGGGG CGGGGAGAGA GCTGGAGCCC CCCCAGGGGC CCTCCAGTCT GCCTGAGCTC 
CAGGATGAGG AGCCCTGCGC ACAGGCTCAG CGGCCGTCCC CCCCGGAGCC CTGGGGCGAT 
GAGGAGCTGC CTCCCCACAG CTCCTTCTTC GGCTCCGAGG GCCGGACAGT GACCTGCGTG 
ACTGTGGTGG AGCCCGACAC GCCGCCCAGC CCAGACGCCC CGCCTGCCAC CACCCACAGG 
GTCGTGGAGC TGCGCTCCCT CTCCCGCTCC CGCTCCCGCT CCAGTTCCCG CAGCCGGAAG 
AAAGGCCCGC AGCCGAGGGC GGCCACCAGG GAGCACCGTG CCGGCCGCTC AGGCTCCCGC 
TCTTCCCGCT CAGGAGACCG GAGTTCCAGG TCTGCCTCGC CGCCGGCGGG CGAGGACCAG 
CCCAAGGGAC ACCGGCCCAA GGCCCGGGGC CGCAGGTCCT CCAGCGAGCA CTCCAGCAGC 
CGCGAGCGTG CCAAGCGGAA GAAGGCGAAG GACAAGAGCA GGGAGCGCGG CAGAGGCTCC 
TGGGGTCGCG GCCGTCGGAG GTCCCGCTCC CGCTCTGGGA GCCCTGGGAG CTCGTCCCAC 
GAGCACCGCG AGGGCCGCAG GAAGAAGAGG CGACGGTCAG GGTCCAGGTC TCGGGGGAGG 
GAGTGCTCGC CCCCTAGGCA CCCCAGGGAG AGGAGGGGGC GGCCCCGAGA CAGGCGCGGC 
TCCCGCGAGA GGCGGCGGCG AAGGTCCAGG TCCCCCAGCT CGGAACACCG GCCCCGGGCG 
CACCGGCGGC CTCGGTCCCG GGAGAAGCGC TCGCGGCCCC GCCCCCACTC CCCTGAGCGG 
AAGTTGGCCC CGAAAGAGGC TTCTGCAGCA CCCGCTCCCC AGGGGGAGCC CAGGCCAGAC 
AGGGAGCCCC CAGCCAGGTC CCCGGCTAGA CCCCCGTCCT CAGCGGGAGC AGACGGCTTG 
CCAGAAGGGC CCGAAGTCCA CAAGACTGCC CCGGTCCCGG AGGTGCTGGC CGCGTGTCCA 
GCGGAGGACG TGGACTATGG CGACTCTGTG GAGGCGGGGC ACCTCTTTGA GGACTTTTCC 
AGTGACACCA TCTTCATGCA GCTTGATGAT ATGAGTTCCC CGCCCTCCCC GGAGAGCACG 
GACTCCTCCC CAGAGCGTGA CCTCCTGCCC AAGACCACCA TGCCCCCAGC TGGCCCCCAG 
CACGACACCA GCCTGGCCAT GGCCATCATC CGGAGGGAGG TGTCTCGGAT CCACGGTGAA 
GATGTGGCAC AGCCCTCGCC TGGGACCGAG GGCCCCCAGG AGAAGCACTT GCTCCAGCAG 
GACGCAGACG AGGCCGCCGC AGTGCCCTGT GCCCTGGGAG CCCAGGCCCC GGGCGGGATG 
CCTGTGGGGA AGGAGGAAGG CCCCTCTCAG AACCCCCTGC TGCGGGCTAA GGCGCTGGTG 
AAGAGGGTCA CCTGGAACCT CCAGGAGGCA GAGTGCGGGG CCCCGGCCAA TGACAGAGGC 
CTGTGGACAC CACTTCACAG GCCACAAAAG CCCCGGGAAG GAGTCTGGGA ATCGGACGAC 
GTGGGCCCCA TGGCTGGGTT CCAGCAGGCA CCCTTCTCTG AGCCGCCGGC CCCTGCTTAT 
GCGCTTCCAG AGCCTGGCTT CCCCTACACT GACCCCTCTC AGGTACAGAG CCCCAGCCTG 
CCCCCTGGCC CAGCCCTGCC ATCGGGCATC CCGCCTTATG CACCTATCAG CCAGCCCGCG 
GTCCAGTTCC TCCTCCAGGG GAGCCTGCCC CTCCCGGGCT GCGGGGCGGC CCAGAGCATG 
GCCCCAGTGT CCACCACCCC GACTGCAGCC TCAGAGCCAG CTGGCCACGC TGCCGCCACC 
ACTGCTGCCA ACAACTCTGA GGAGAGGACG GCTCCCCCCA GGCCGACCCC AGAGAAGGCC 
AAGAATGAGG AGTACATGAA GAAGCTGCAC GTGCAGGAGC GGGCCGTGGA GGAGGTGAAG 
CTGGCCATCA AGCCCTTCTA CCAGAAGAGG GAGGTGACCA AGGAGGAGTA TAAGGACATC 
CTGCGCAAGG CGGTGCAGAA GATTTGCCAC AGCAAGAGCG GGGAGATCAA CCCCGTGAAG 
GTGGGCAACC TGGTGAAGGC CTACGTGGAC AAGTACCGGC ACATGCGCAG ACACCGGCGG 
GCCGAGGCCG GCGAGGAGCC GCCCGCCCAG AGCGCCGAGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227313.1
CDS	152..4936
Translation	MDDDSLDELVDRSPGPDGHPRLRPAALASDAEGSSDAHSDASEDDSGSEHSDEEDADGDLEDGSGSEDSEDDGDDLDALAAAADSQGALEGSGAFNSDDDSESCPICLNAFREQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRAQFGGKILKKIPVESAHLGENENEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECATPGAASAADAGPVSEEEVSLLLADVVPTTSRLRPRTGRTRAIARTRQSERVRATVNRNRISTARRFQRVPRYLMSSLLDETIEAVAAGLSTAVYQRPGPRAPARRRRKGGRRKKTSGRKKAPSRLSAKSRSSGARLKKRPGRVKKRKAQKIKNEATARSRIARTLGLCRPACGACTPSVYKPADPSLGLMRADIGAASLSLFGDPYELDPFDSSEEASASPASPLSAKRRVLSRSALRSHQPVARPISMGLSRRGAPAVAPQPEVDTEPSPDLLGSILSGQSLLMMNGADIIIHRDGSLSAKRAVPVSFQQNSAGPSREPEGAWGRQSGSGPQSSGPSCPGRAAPAPTRVPALTSGAAAKPDSSVTPRLGQSQTSSSESRPGIQQSEGGQCNGDSKHSLPLGPAALKASHSSSDSPSKSTVPGQAPRPAPRRTDISELPRIPKIRRDSSRGDEGPASRQGVELPSSCISRLTGREGPGQPGCGARVEGEPSNRGAQEPSTHSGGGPQPPGPLGPARGKAVGSTFESFRINIPGNTAHSGRLLSPGFCNTFRPVDSKVQRRASPSPLFSVRKTKQLKSEIYDPFNPTGSDSSSANSSPERLGLLPSEITRTISVDSPKAPVLPPVRCVTSYTVQTGAGRELEPPQGPSSLPELQDEEPCAQAQRPSPPEPWGDEELPPHSSFFGSEGRTVTCVTVVEPDTPPSPDAPPATTHRVVELRSLSRSRSRSSSRSRKKGPQPRAATREHRAGRSGSRSSRSGDRSSRSASPPAGEDQPKGHRPKARGRRSSSEHSSSRERAKRKKAKDKSRERGRGSWGRGRRRSRSRSGSPGSSSHEHREGRRKKRRRSGSRSRGRECSPPRHPRERRGRPRDRRGSRERRRRRSRSPSSEHRPRAHRRPRSREKRSRPRPHSPERKLAPKEASAAPAPQGEPRPDREPPARSPARPPSSAGADGLPEGPEVHKTAPVPEVLAACPAEDVDYGDSVEAGHLFEDFSSDTIFMQLDDMSSPPSPESTDSSPERDLLPKTTMPPAGPQHDTSLAMAIIRREVSRIHGEDVAQPSPGTEGPQEKHLLQQDADEAAAVPCALGAQAPGGMPVGKEEGPSQNPLLRAKALVKRVTWNLQEAECGAPANDRGLWTPLHRPQKPREGVWESDDVGPMAGFQQAPFSEPPAPAYALPEPGFPYTDPSQVQSPSLPPGPALPSGIPPYAPISQPAVQFLLQGSLPLPGCGAAQSMAPVSTTPTAASEPAGHAAATTAANNSEERTAPPRPTPEKAKNEEYMKKLHVQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEPPAQSAEG

Target ORF information:

RefSeq Version	XM_005227256.4
Organism	Bos taurus(cattle)
Definition	Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227256.4

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA CGAGCTTGTG GACCGAAGCC CGGGGCCCGA TGGCCACCCC 
AGACTCCGTC CTGCCGCCCT GGCCAGCGAT GCTGAAGGAA GCAGTGATGC CCACAGCGAC 
GCCTCTGAGG ACGACTCTGG CAGCGAGCAC AGCGATGAGG AGGATGCGGA CGGGGACCTG 
GAGGACGGAT CTGGTTCCGA GGACTCTGAG GACGATGGTG ACGACCTGGA CGCGTTGGCA 
GCGGCGGCGG ATTCTCAGGG AGCTCTGGAA GGCAGCGGTG CGTTCAATTC TGATGACGAC 
TCCGAGAGCT GCCCGATCTG TCTCAATGCT TTCAGGGAGC AGGCTGTGGG AACGCCCGAG 
AACTGCGCCC ACTACTTCTG CCTCGATTGC ATTGTGGAGT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTAT ATTTAAGTGT ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTGAAGA AGATTCCAGT AGAGAGTGCC CACCTTGGGG AGAACGAGAA TGAAGACCCG 
ACCTTCTGTG AGGTGTGTGG CCGGAGCGAC CGGGAGGACC GGCTGCTGCT CTGTGACGGC 
TGCGACGCTG GGTACCACAT GGAGTGCTTG GACCCCCCTC TCCAGGAGGT TCCAGTGGAT 
GAGTGGTTCT GTCCGGAATG TGCTACCCCC GGGGCTGCTT CTGCCGCTGA TGCGGGTCCT 
GTGAGCGAGG AAGAGGTCTC CCTGCTCTTG GCTGACGTGG TGCCCACCAC CAGCCGGCTT 
CGGCCCCGCA CGGGGAGGAC ACGGGCCATC GCCAGGACGA GGCAGAGCGA GAGGGTGCGA 
GCGACAGTGA ACCGGAACCG GATCTCCACG GCCAGGAGGT TCCAGCGTGT CCCCAGGTAC 
CTCATGTCTT CTCTGCTGGA TGAGACCATT GAAGCCGTGG CTGCCGGGCT CAGCACGGCT 
GTGTACCAGC GCCCCGGGCC GCGTGCCCCA GCCCGCCGCA GGAGGAAAGG AGGAAGACGG 
AAGAAAACGT CAGGAAGAAA GAAAGCCCCG TCCAGATTGT CTGCAAAGAG CAGGAGCTCA 
GGGGCAAGGT TGAAGAAACG CCCAGGTCGT GTGAAGAAGA GGAAAGCACA GAAGATAAAG 
AATGAAGCCA CGGCTCGTTC GCGCATCGCA CGGACGCTGG GCCTCTGCAG GCCGGCCTGT 
GGAGCCTGCA CCCCCTCCGT GTACAAGCCT GCAGACCCGT CTCTGGGGCT GATGCGAGCC 
GACATCGGGG CAGCCTCCCT CTCCCTGTTT GGAGACCCCT ATGAATTGGA TCCCTTTGAC 
AGCAGTGAAG AGGCGTCTGC AAGTCCTGCT TCCCCTCTGA GCGCCAAGAG AAGGGTTCTT 
TCCCGATCAG CCCTGCGCTC CCACCAGCCT GTAGCCAGAC CCATCTCCAT GGGGCTCTCC 
AGGAGAGGTG CTCCTGCCGT GGCCCCCCAG CCGGAAGTGG ACACAGAGCC CAGCCCTGAC 
CTGCTGGGGA GCATCCTGTC TGGCCAGAGC CTTCTGATGA TGAACGGTGC CGACATCATC 
ATCCACCGCG ACGGCTCCCT CAGCGCCAAG AGGGCAGTGC CAGTCTCTTT TCAGCAGAAC 
TCAGCTGGTC CATCCAGAGA GCCAGAAGGC GCCTGGGGGC GTCAGTCAGG AAGCGGGCCA 
CAGAGCTCGG GGCCCAGCTG CCCGGGCAGG GCCGCCCCCG CCCCCACCCG TGTCCCTGCA 
CTCACATCGG GGGCAGCGGC AAAGCCGGAC TCCTCGGTGA CCCCTCGGTT GGGCCAGTCT 
CAGACCTCGT CCAGCGAGAG CAGACCTGGT ATACAGCAGA GTGAGGGTGG CCAGTGTAAC 
GGTGACAGCA AGCACTCTCT GCCTCTTGGG CCTGCGGCGC TGAAGGCCTC ACACAGCAGT 
TCTGACTCGC CCTCCAAGAG TACAGTGCCG GGCCAGGCCC CGAGACCAGC TCCCAGGCGG 
ACAGACATCT CCGAGCTCCC CAGGATACCA AAGATCAGGA GAGACAGCAG CCGGGGGGAC 
GAGGGCCCTG CCAGTAGGCA GGGCGTCGAG CTCCCCAGCT CCTGCATCAG CCGGCTGACA 
GGCAGGGAGG GCCCTGGACA GCCTGGCTGC GGGGCCCGGG TGGAGGGTGA GCCCAGCAAC 
CGGGGCGCAC AGGAGCCCAG CACGCACTCC GGGGGGGGCC CCCAGCCCCC CGGCCCGCTG 
GGGCCGGCCC GGGGGAAGGC TGTTGGCTCC ACCTTTGAGA GTTTCAGGAT CAACATTCCT 
GGGAACACAG CCCATTCTGG CAGACTGCTC AGCCCTGGCT TCTGTAACAC GTTCCGGCCG 
GTGGACAGCA AGGTGCAGAG GAGAGCGAGC CCCTCGCCCC TCTTCTCTGT CAGGAAGACG 
AAGCAGCTCA AGAGCGAGAT CTATGACCCC TTCAACCCCA CGGGCTCCGA CTCCAGCTCT 
GCCAACAGCA GCCCCGAGCG TCTGGGCCTC CTGCCCTCGG AGATCACGCG GACCATCTCT 
GTTGACAGCC CCAAGGCCCC GGTGCTGCCC CCCGTGCGCT GCGTCACCTC CTACACGGTG 
CAGACAGGGG CGGGGAGAGA GCTGGAGCCC CCCCAGGGGC CCTCCAGTCT GCCTGAGCTC 
CAGGATGAGG AGCCCTGCGC ACAGGCTCAG CGGCCGTCCC CCCCGGAGCC CTGGGGCGAT 
GAGGAGCTGC CTCCCCACAG CTCCTTCTTC GGCTCCGAGG GCCGGACAGT GACCTGCGTG 
ACTGTGGTGG AGCCCGACAC GCCGCCCAGC CCAGACGCCC CGCCTGCCAC CACCCACAGG 
GTCGTGGAGC TGCGCTCCCT CTCCCGCTCC CGCTCCCGCT CCAGTTCCCG CAGCCGGAAG 
AAAGGCCCGC AGCCGAGGGC GGCCACCAGG GAGCACCGTG CCGGCCGCTC AGGCTCCCGC 
TCTTCCCGCT CAGGAGACCG GAGTTCCAGG TCTGCCTCGC CGCCGGCGGG CGAGGACCAG 
CCCAAGGGAC ACCGGCCCAA GGCCCGGGGC CGCAGGTCCT CCAGCGAGCA CTCCAGCAGC 
CGCGAGCGTG CCAAGCGGAA GAAGGCGAAG GACAAGAGCA GGGAGCGCGG CAGAGGCTCC 
TGGGGTCGCG GCCGTCGGAG GTCCCGCTCC CGCTCTGGGA GCCCTGGGAG CTCGTCCCAC 
GAGCACCGCG AGGGCCGCAG GAAGAAGAGG CGACGGTCAG GGTCCAGGTC TCGGGGGAGG 
GAGTGCTCGC CCCCTAGGCA CCCCAGGGAG AGGAGGGGGC GGCCCCGAGA CAGGCGCGGC 
TCCCGCGAGA GGCGGCGGCG AAGGTCCAGG TCCCCCAGCT CGGAACACCG GCCCCGGGCG 
CACCGGCGGC CTCGGTCCCG GGAGAAGCGC TCGCGGCCCC GCCCCCACTC CCCTGAGCGG 
AAGTTGGCCC CGAAAGAGGC TTCTGCAGCA CCCGCTCCCC AGGGGGAGCC CAGGCCAGAC 
AGGGAGCCCC CAGCCAGGTC CCCGGCTAGA CCCCCGTCCT CAGCGGGAGC AGACGGCTTG 
CCAGAAGGGC CCGAAGTCCA CAAGACTGCC CCGGTCCCGG AGGTGCTGGC CGCGTGTCCA 
GCGGAGGACG TGGACTATGG CGACTCTGTG GAGGCGGGGC ACCTCTTTGA GGACTTTTCC 
AGTGACACCA TCTTCATGCA GCTTGATGAT ATGAGTTCCC CGCCCTCCCC GGAGAGCACG 
GACTCCTCCC CAGAGCGTGA CCTCCTGCCC AAGACCACCA TGCCCCCAGC TGGCCCCCAG 
CACGACACCA GCCTGGCCAT GGCCATCATC CGGAGGGAGG TGTCTCGGAT CCACGGTGAA 
GATGTGGCAC AGCCCTCGCC TGGGACCGAG GGCCCCCAGG AGAAGCACTT GCTCCAGCAG 
GACGCAGACG AGGCCGCCGC AGTGCCCTGT GCCCTGGGAG CCCAGGCCCC GGGCGGGATG 
CCTGTGGGGA AGGAGGAAGG CCCCTCTCAG AACCCCCTGC TGCGGGCTAA GGCGCTGGTG 
AAGAGGGTCA CCTGGAACCT CCAGGAGGCA GAGTGCGGGG CCCCGGCCAA TGACAGAGGC 
CTGTGGACAC CACTTCACAG GCCACAAAAG CCCCGGGAAG GAGTCTGGGA ATCGGACGAC 
GTGGGCCCCA TGGCTGGGTT CCAGCAGGCA CCCTTCTCTG AGCCGCCGGC CCCTGCTTAT 
GCGCTTCCAG AGCCTGGCTT CCCCTACACT GACCCCTCTC AGGTACAGAG CCCCAGCCTG 
CCCCCTGGCC CAGCCCTGCC ATCGGGCATC CCGCCTTATG CACCTATCAG CCAGCCCGCG 
GTCCAGTTCC TCCTCCAGGG GAGCCTGCCC CTCCCGGGCT GCGGGGCGGC CCAGAGCATG 
GCCCCAGTGT CCACCACCCC GACTGCAGCC TCAGAGCCAG CTGGCCACGC TGCCGCCACC 
ACTGCTGCCA ACAACTCTGA GGAGAGGACG GCTCCCCCCA GGCCGACCCC AGAGAAGGCC 
AAGAATGAGG AGTACATGAA GAAGCTGCAC GTGCAGGAGC GGGCCGTGGA GGAGGTGAAG 
CTGGCCATCA AGCCCTTCTA CCAGAAGAGG GAGGTGACCA AGGAGGAGTA TAAGGACATC 
CTGCGCAAGG CGGTGCAGAA GATTTGCCAC AGCAAGAGCG GGGAGATCAA CCCCGTGAAG 
GTGGGCAACC TGGTGAAGGC CTACGTGGAC AAGTACCGGC ACATGCGCAG ACACCGGCGG 
GCCGAGGCCG GCGAGGAGCC GCCCGCCCAG AGCGCCGAGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_005227256.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227313.1 Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

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CloneID	OBa34951
Clone ID Related Accession (Same CDS sequence)	XM_005227256.4 , XM_005227256.3
Accession Version	XM_005227256.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCCTGGA CGAGCTTGTG GACCGAAGCC CGGGGCCCGA TGGCCACCCC 
AGACTCCGTC CTGCCGCCCT GGCCAGCGAT GCTGAAGGAA GCAGTGATGC CCACAGCGAC 
GCCTCTGAGG ACGACTCTGG CAGCGAGCAC AGCGATGAGG AGGATGCGGA CGGGGACCTG 
GAGGACGGAT CTGGTTCCGA GGACTCTGAG GACGATGGTG ACGACCTGGA CGCGTTGGCA 
GCGGCGGCGG ATTCTCAGGG AGCTCTGGAA GGCAGCGGTG CGTTCAATTC TGATGACGAC 
TCCGAGAGCT GCCCGATCTG TCTCAATGCT TTCAGGGAGC AGGCTGTGGG AACGCCCGAG 
AACTGCGCCC ACTACTTCTG CCTCGATTGC ATTGTGGAGT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTAT ATTTAAGTGT ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTGAAGA AGATTCCAGT AGAGAGTGCC CACCTTGGGG AGAACGAGAA TGAAGACCCG 
ACCTTCTGTG AGGTGTGTGG CCGGAGCGAC CGGGAGGACC GGCTGCTGCT CTGTGACGGC 
TGCGACGCTG GGTACCACAT GGAGTGCTTG GACCCCCCTC TCCAGGAGGT TCCAGTGGAT 
GAGTGGTTCT GTCCGGAATG TGCTACCCCC GGGGCTGCTT CTGCCGCTGA TGCGGGTCCT 
GTGAGCGAGG AAGAGGTCTC CCTGCTCTTG GCTGACGTGG TGCCCACCAC CAGCCGGCTT 
CGGCCCCGCA CGGGGAGGAC ACGGGCCATC GCCAGGACGA GGCAGAGCGA GAGGGTGCGA 
GCGACAGTGA ACCGGAACCG GATCTCCACG GCCAGGAGGT TCCAGCGTGT CCCCAGGTAC 
CTCATGTCTT CTCTGCTGGA TGAGACCATT GAAGCCGTGG CTGCCGGGCT CAGCACGGCT 
GTGTACCAGC GCCCCGGGCC GCGTGCCCCA GCCCGCCGCA GGAGGAAAGG AGGAAGACGG 
AAGAAAACGT CAGGAAGAAA GAAAGCCCCG TCCAGATTGT CTGCAAAGAG CAGGAGCTCA 
GGGGCAAGGT TGAAGAAACG CCCAGGTCGT GTGAAGAAGA GGAAAGCACA GAAGATAAAG 
AATGAAGCCA CGGCTCGTTC GCGCATCGCA CGGACGCTGG GCCTCTGCAG GCCGGCCTGT 
GGAGCCTGCA CCCCCTCCGT GTACAAGCCT GCAGACCCGT CTCTGGGGCT GATGCGAGCC 
GACATCGGGG CAGCCTCCCT CTCCCTGTTT GGAGACCCCT ATGAATTGGA TCCCTTTGAC 
AGCAGTGAAG AGGCGTCTGC AAGTCCTGCT TCCCCTCTGA GCGCCAAGAG AAGGGTTCTT 
TCCCGATCAG CCCTGCGCTC CCACCAGCCT GTAGCCAGAC CCATCTCCAT GGGGCTCTCC 
AGGAGAGGTG CTCCTGCCGT GGCCCCCCAG CCGGAAGTGG ACACAGAGCC CAGCCCTGAC 
CTGCTGGGGA GCATCCTGTC TGGCCAGAGC CTTCTGATGA TGAACGGTGC CGACATCATC 
ATCCACCGCG ACGGCTCCCT CAGCGCCAAG AGGGCAGTGC CAGTCTCTTT TCAGCAGAAC 
TCAGCTGGTC CATCCAGAGA GCCAGAAGGC GCCTGGGGGC GTCAGTCAGG AAGCGGGCCA 
CAGAGCTCGG GGCCCAGCTG CCCGGGCAGG GCCGCCCCCG CCCCCACCCG TGTCCCTGCA 
CTCACATCGG GGGCAGCGGC AAAGCCGGAC TCCTCGGTGA CCCCTCGGTT GGGCCAGTCT 
CAGACCTCGT CCAGCGAGAG CAGACCTGGT ATACAGCAGA GTGAGGGTGG CCAGTGTAAC 
GGTGACAGCA AGCACTCTCT GCCTCTTGGG CCTGCGGCGC TGAAGGCCTC ACACAGCAGT 
TCTGACTCGC CCTCCAAGAG TACAGTGCCG GGCCAGGCCC CGAGACCAGC TCCCAGGCGG 
ACAGACATCT CCGAGCTCCC CAGGATACCA AAGATCAGGA GAGACAGCAG CCGGGGGGAC 
GAGGGCCCTG CCAGTAGGCA GGGCGTCGAG CTCCCCAGCT CCTGCATCAG CCGGCTGACA 
GGCAGGGAGG GCCCTGGACA GCCTGGCTGC GGGGCCCGGG TGGAGGGTGA GCCCAGCAAC 
CGGGGCGCAC AGGAGCCCAG CACGCACTCC GGGGGGGGCC CCCAGCCCCC CGGCCCGCTG 
GGGCCGGCCC GGGGGAAGGC TGTTGGCTCC ACCTTTGAGA GTTTCAGGAT CAACATTCCT 
GGGAACACAG CCCATTCTGG CAGACTGCTC AGCCCTGGCT TCTGTAACAC GTTCCGGCCG 
GTGGACAGCA AGGTGCAGAG GAGAGCGAGC CCCTCGCCCC TCTTCTCTGT CAGGAAGACG 
AAGCAGCTCA AGAGCGAGAT CTATGACCCC TTCAACCCCA CGGGCTCCGA CTCCAGCTCT 
GCCAACAGCA GCCCCGAGCG TCTGGGCCTC CTGCCCTCGG AGATCACGCG GACCATCTCT 
GTTGACAGCC CCAAGGCCCC GGTGCTGCCC CCCGTGCGCT GCGTCACCTC CTACACGGTG 
CAGACAGGGG CGGGGAGAGA GCTGGAGCCC CCCCAGGGGC CCTCCAGTCT GCCTGAGCTC 
CAGGATGAGG AGCCCTGCGC ACAGGCTCAG CGGCCGTCCC CCCCGGAGCC CTGGGGCGAT 
GAGGAGCTGC CTCCCCACAG CTCCTTCTTC GGCTCCGAGG GCCGGACAGT GACCTGCGTG 
ACTGTGGTGG AGCCCGACAC GCCGCCCAGC CCAGACGCCC CGCCTGCCAC CACCCACAGG 
GTCGTGGAGC TGCGCTCCCT CTCCCGCTCC CGCTCCCGCT CCAGTTCCCG CAGCCGGAAG 
AAAGGCCCGC AGCCGAGGGC GGCCACCAGG GAGCACCGTG CCGGCCGCTC AGGCTCCCGC 
TCTTCCCGCT CAGGAGACCG GAGTTCCAGG TCTGCCTCGC CGCCGGCGGG CGAGGACCAG 
CCCAAGGGAC ACCGGCCCAA GGCCCGGGGC CGCAGGTCCT CCAGCGAGCA CTCCAGCAGC 
CGCGAGCGTG CCAAGCGGAA GAAGGCGAAG GACAAGAGCA GGGAGCGCGG CAGAGGCTCC 
TGGGGTCGCG GCCGTCGGAG GTCCCGCTCC CGCTCTGGGA GCCCTGGGAG CTCGTCCCAC 
GAGCACCGCG AGGGCCGCAG GAAGAAGAGG CGACGGTCAG GGTCCAGGTC TCGGGGGAGG 
GAGTGCTCGC CCCCTAGGCA CCCCAGGGAG AGGAGGGGGC GGCCCCGAGA CAGGCGCGGC 
TCCCGCGAGA GGCGGCGGCG AAGGTCCAGG TCCCCCAGCT CGGAACACCG GCCCCGGGCG 
CACCGGCGGC CTCGGTCCCG GGAGAAGCGC TCGCGGCCCC GCCCCCACTC CCCTGAGCGG 
AAGTTGGCCC CGAAAGAGGC TTCTGCAGCA CCCGCTCCCC AGGGGGAGCC CAGGCCAGAC 
AGGGAGCCCC CAGCCAGGTC CCCGGCTAGA CCCCCGTCCT CAGCGGGAGC AGACGGCTTG 
CCAGAAGGGC CCGAAGTCCA CAAGACTGCC CCGGTCCCGG AGGTGCTGGC CGCGTGTCCA 
GCGGAGGACG TGGACTATGG CGACTCTGTG GAGGCGGGGC ACCTCTTTGA GGACTTTTCC 
AGTGACACCA TCTTCATGCA GCTTGATGAT ATGAGTTCCC CGCCCTCCCC GGAGAGCACG 
GACTCCTCCC CAGAGCGTGA CCTCCTGCCC AAGACCACCA TGCCCCCAGC TGGCCCCCAG 
CACGACACCA GCCTGGCCAT GGCCATCATC CGGAGGGAGG TGTCTCGGAT CCACGGTGAA 
GATGTGGCAC AGCCCTCGCC TGGGACCGAG GGCCCCCAGG AGAAGCACTT GCTCCAGCAG 
GACGCAGACG AGGCCGCCGC AGTGCCCTGT GCCCTGGGAG CCCAGGCCCC GGGCGGGATG 
CCTGTGGGGA AGGAGGAAGG CCCCTCTCAG AACCCCCTGC TGCGGGCTAA GGCGCTGGTG 
AAGAGGGTCA CCTGGAACCT CCAGGAGGCA GAGTGCGGGG CCCCGGCCAA TGACAGAGGC 
CTGTGGACAC CACTTCACAG GCCACAAAAG CCCCGGGAAG GAGTCTGGGA ATCGGACGAC 
GTGGGCCCCA TGGCTGGGTT CCAGCAGGCA CCCTTCTCTG AGCCGCCGGC CCCTGCTTAT 
GCGCTTCCAG AGCCTGGCTT CCCCTACACT GACCCCTCTC AGGTACAGAG CCCCAGCCTG 
CCCCCTGGCC CAGCCCTGCC ATCGGGCATC CCGCCTTATG CACCTATCAG CCAGCCCGCG 
GTCCAGTTCC TCCTCCAGGG GAGCCTGCCC CTCCCGGGCT GCGGGGCGGC CCAGAGCATG 
GCCCCAGTGT CCACCACCCC GACTGCAGCC TCAGAGCCAG CTGGCCACGC TGCCGCCACC 
ACTGCTGCCA ACAACTCTGA GGAGAGGACG GCTCCCCCCA GGCCGACCCC AGAGAAGGCC 
AAGAATGAGG AGTACATGAA GAAGCTGCAC GTGCAGGAGC GGGCCGTGGA GGAGGTGAAG 
CTGGCCATCA AGCCCTTCTA CCAGAAGAGG GAGGTGACCA AGGAGGAGTA TAAGGACATC 
CTGCGCAAGG CGGTGCAGAA GATTTGCCAC AGCAAGAGCG GGGAGATCAA CCCCGTGAAG 
GTGGGCAACC TGGTGAAGGC CTACGTGGAC AAGTACCGGC ACATGCGCAG ACACCGGCGG 
GCCGAGGCCG GCGAGGAGCC GCCCGCCCAG AGCGCCGAGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227313.1
CDS	147..4931
Translation	MDDDSLDELVDRSPGPDGHPRLRPAALASDAEGSSDAHSDASEDDSGSEHSDEEDADGDLEDGSGSEDSEDDGDDLDALAAAADSQGALEGSGAFNSDDDSESCPICLNAFREQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRAQFGGKILKKIPVESAHLGENENEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECATPGAASAADAGPVSEEEVSLLLADVVPTTSRLRPRTGRTRAIARTRQSERVRATVNRNRISTARRFQRVPRYLMSSLLDETIEAVAAGLSTAVYQRPGPRAPARRRRKGGRRKKTSGRKKAPSRLSAKSRSSGARLKKRPGRVKKRKAQKIKNEATARSRIARTLGLCRPACGACTPSVYKPADPSLGLMRADIGAASLSLFGDPYELDPFDSSEEASASPASPLSAKRRVLSRSALRSHQPVARPISMGLSRRGAPAVAPQPEVDTEPSPDLLGSILSGQSLLMMNGADIIIHRDGSLSAKRAVPVSFQQNSAGPSREPEGAWGRQSGSGPQSSGPSCPGRAAPAPTRVPALTSGAAAKPDSSVTPRLGQSQTSSSESRPGIQQSEGGQCNGDSKHSLPLGPAALKASHSSSDSPSKSTVPGQAPRPAPRRTDISELPRIPKIRRDSSRGDEGPASRQGVELPSSCISRLTGREGPGQPGCGARVEGEPSNRGAQEPSTHSGGGPQPPGPLGPARGKAVGSTFESFRINIPGNTAHSGRLLSPGFCNTFRPVDSKVQRRASPSPLFSVRKTKQLKSEIYDPFNPTGSDSSSANSSPERLGLLPSEITRTISVDSPKAPVLPPVRCVTSYTVQTGAGRELEPPQGPSSLPELQDEEPCAQAQRPSPPEPWGDEELPPHSSFFGSEGRTVTCVTVVEPDTPPSPDAPPATTHRVVELRSLSRSRSRSSSRSRKKGPQPRAATREHRAGRSGSRSSRSGDRSSRSASPPAGEDQPKGHRPKARGRRSSSEHSSSRERAKRKKAKDKSRERGRGSWGRGRRRSRSRSGSPGSSSHEHREGRRKKRRRSGSRSRGRECSPPRHPRERRGRPRDRRGSRERRRRRSRSPSSEHRPRAHRRPRSREKRSRPRPHSPERKLAPKEASAAPAPQGEPRPDREPPARSPARPPSSAGADGLPEGPEVHKTAPVPEVLAACPAEDVDYGDSVEAGHLFEDFSSDTIFMQLDDMSSPPSPESTDSSPERDLLPKTTMPPAGPQHDTSLAMAIIRREVSRIHGEDVAQPSPGTEGPQEKHLLQQDADEAAAVPCALGAQAPGGMPVGKEEGPSQNPLLRAKALVKRVTWNLQEAECGAPANDRGLWTPLHRPQKPREGVWESDDVGPMAGFQQAPFSEPPAPAYALPEPGFPYTDPSQVQSPSLPPGPALPSGIPPYAPISQPAVQFLLQGSLPLPGCGAAQSMAPVSTTPTAASEPAGHAAATTAANNSEERTAPPRPTPEKAKNEEYMKKLHVQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEPPAQSAEG

Target ORF information:

RefSeq Version	XM_005227256.3
Organism	Bos taurus(cattle)
Definition	Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227256.3

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA CGAGCTTGTG GACCGAAGCC CGGGGCCCGA TGGCCACCCC 
AGACTCCGTC CTGCCGCCCT GGCCAGCGAT GCTGAAGGAA GCAGTGATGC CCACAGCGAC 
GCCTCTGAGG ACGACTCTGG CAGCGAGCAC AGCGATGAGG AGGATGCGGA CGGGGACCTG 
GAGGACGGAT CTGGTTCCGA GGACTCTGAG GACGATGGTG ACGACCTGGA CGCGTTGGCA 
GCGGCGGCGG ATTCTCAGGG AGCTCTGGAA GGCAGCGGTG CGTTCAATTC TGATGACGAC 
TCCGAGAGCT GCCCGATCTG TCTCAATGCT TTCAGGGAGC AGGCTGTGGG AACGCCCGAG 
AACTGCGCCC ACTACTTCTG CCTCGATTGC ATTGTGGAGT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTAT ATTTAAGTGT ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTGAAGA AGATTCCAGT AGAGAGTGCC CACCTTGGGG AGAACGAGAA TGAAGACCCG 
ACCTTCTGTG AGGTGTGTGG CCGGAGCGAC CGGGAGGACC GGCTGCTGCT CTGTGACGGC 
TGCGACGCTG GGTACCACAT GGAGTGCTTG GACCCCCCTC TCCAGGAGGT TCCAGTGGAT 
GAGTGGTTCT GTCCGGAATG TGCTACCCCC GGGGCTGCTT CTGCCGCTGA TGCGGGTCCT 
GTGAGCGAGG AAGAGGTCTC CCTGCTCTTG GCTGACGTGG TGCCCACCAC CAGCCGGCTT 
CGGCCCCGCA CGGGGAGGAC ACGGGCCATC GCCAGGACGA GGCAGAGCGA GAGGGTGCGA 
GCGACAGTGA ACCGGAACCG GATCTCCACG GCCAGGAGGT TCCAGCGTGT CCCCAGGTAC 
CTCATGTCTT CTCTGCTGGA TGAGACCATT GAAGCCGTGG CTGCCGGGCT CAGCACGGCT 
GTGTACCAGC GCCCCGGGCC GCGTGCCCCA GCCCGCCGCA GGAGGAAAGG AGGAAGACGG 
AAGAAAACGT CAGGAAGAAA GAAAGCCCCG TCCAGATTGT CTGCAAAGAG CAGGAGCTCA 
GGGGCAAGGT TGAAGAAACG CCCAGGTCGT GTGAAGAAGA GGAAAGCACA GAAGATAAAG 
AATGAAGCCA CGGCTCGTTC GCGCATCGCA CGGACGCTGG GCCTCTGCAG GCCGGCCTGT 
GGAGCCTGCA CCCCCTCCGT GTACAAGCCT GCAGACCCGT CTCTGGGGCT GATGCGAGCC 
GACATCGGGG CAGCCTCCCT CTCCCTGTTT GGAGACCCCT ATGAATTGGA TCCCTTTGAC 
AGCAGTGAAG AGGCGTCTGC AAGTCCTGCT TCCCCTCTGA GCGCCAAGAG AAGGGTTCTT 
TCCCGATCAG CCCTGCGCTC CCACCAGCCT GTAGCCAGAC CCATCTCCAT GGGGCTCTCC 
AGGAGAGGTG CTCCTGCCGT GGCCCCCCAG CCGGAAGTGG ACACAGAGCC CAGCCCTGAC 
CTGCTGGGGA GCATCCTGTC TGGCCAGAGC CTTCTGATGA TGAACGGTGC CGACATCATC 
ATCCACCGCG ACGGCTCCCT CAGCGCCAAG AGGGCAGTGC CAGTCTCTTT TCAGCAGAAC 
TCAGCTGGTC CATCCAGAGA GCCAGAAGGC GCCTGGGGGC GTCAGTCAGG AAGCGGGCCA 
CAGAGCTCGG GGCCCAGCTG CCCGGGCAGG GCCGCCCCCG CCCCCACCCG TGTCCCTGCA 
CTCACATCGG GGGCAGCGGC AAAGCCGGAC TCCTCGGTGA CCCCTCGGTT GGGCCAGTCT 
CAGACCTCGT CCAGCGAGAG CAGACCTGGT ATACAGCAGA GTGAGGGTGG CCAGTGTAAC 
GGTGACAGCA AGCACTCTCT GCCTCTTGGG CCTGCGGCGC TGAAGGCCTC ACACAGCAGT 
TCTGACTCGC CCTCCAAGAG TACAGTGCCG GGCCAGGCCC CGAGACCAGC TCCCAGGCGG 
ACAGACATCT CCGAGCTCCC CAGGATACCA AAGATCAGGA GAGACAGCAG CCGGGGGGAC 
GAGGGCCCTG CCAGTAGGCA GGGCGTCGAG CTCCCCAGCT CCTGCATCAG CCGGCTGACA 
GGCAGGGAGG GCCCTGGACA GCCTGGCTGC GGGGCCCGGG TGGAGGGTGA GCCCAGCAAC 
CGGGGCGCAC AGGAGCCCAG CACGCACTCC GGGGGGGGCC CCCAGCCCCC CGGCCCGCTG 
GGGCCGGCCC GGGGGAAGGC TGTTGGCTCC ACCTTTGAGA GTTTCAGGAT CAACATTCCT 
GGGAACACAG CCCATTCTGG CAGACTGCTC AGCCCTGGCT TCTGTAACAC GTTCCGGCCG 
GTGGACAGCA AGGTGCAGAG GAGAGCGAGC CCCTCGCCCC TCTTCTCTGT CAGGAAGACG 
AAGCAGCTCA AGAGCGAGAT CTATGACCCC TTCAACCCCA CGGGCTCCGA CTCCAGCTCT 
GCCAACAGCA GCCCCGAGCG TCTGGGCCTC CTGCCCTCGG AGATCACGCG GACCATCTCT 
GTTGACAGCC CCAAGGCCCC GGTGCTGCCC CCCGTGCGCT GCGTCACCTC CTACACGGTG 
CAGACAGGGG CGGGGAGAGA GCTGGAGCCC CCCCAGGGGC CCTCCAGTCT GCCTGAGCTC 
CAGGATGAGG AGCCCTGCGC ACAGGCTCAG CGGCCGTCCC CCCCGGAGCC CTGGGGCGAT 
GAGGAGCTGC CTCCCCACAG CTCCTTCTTC GGCTCCGAGG GCCGGACAGT GACCTGCGTG 
ACTGTGGTGG AGCCCGACAC GCCGCCCAGC CCAGACGCCC CGCCTGCCAC CACCCACAGG 
GTCGTGGAGC TGCGCTCCCT CTCCCGCTCC CGCTCCCGCT CCAGTTCCCG CAGCCGGAAG 
AAAGGCCCGC AGCCGAGGGC GGCCACCAGG GAGCACCGTG CCGGCCGCTC AGGCTCCCGC 
TCTTCCCGCT CAGGAGACCG GAGTTCCAGG TCTGCCTCGC CGCCGGCGGG CGAGGACCAG 
CCCAAGGGAC ACCGGCCCAA GGCCCGGGGC CGCAGGTCCT CCAGCGAGCA CTCCAGCAGC 
CGCGAGCGTG CCAAGCGGAA GAAGGCGAAG GACAAGAGCA GGGAGCGCGG CAGAGGCTCC 
TGGGGTCGCG GCCGTCGGAG GTCCCGCTCC CGCTCTGGGA GCCCTGGGAG CTCGTCCCAC 
GAGCACCGCG AGGGCCGCAG GAAGAAGAGG CGACGGTCAG GGTCCAGGTC TCGGGGGAGG 
GAGTGCTCGC CCCCTAGGCA CCCCAGGGAG AGGAGGGGGC GGCCCCGAGA CAGGCGCGGC 
TCCCGCGAGA GGCGGCGGCG AAGGTCCAGG TCCCCCAGCT CGGAACACCG GCCCCGGGCG 
CACCGGCGGC CTCGGTCCCG GGAGAAGCGC TCGCGGCCCC GCCCCCACTC CCCTGAGCGG 
AAGTTGGCCC CGAAAGAGGC TTCTGCAGCA CCCGCTCCCC AGGGGGAGCC CAGGCCAGAC 
AGGGAGCCCC CAGCCAGGTC CCCGGCTAGA CCCCCGTCCT CAGCGGGAGC AGACGGCTTG 
CCAGAAGGGC CCGAAGTCCA CAAGACTGCC CCGGTCCCGG AGGTGCTGGC CGCGTGTCCA 
GCGGAGGACG TGGACTATGG CGACTCTGTG GAGGCGGGGC ACCTCTTTGA GGACTTTTCC 
AGTGACACCA TCTTCATGCA GCTTGATGAT ATGAGTTCCC CGCCCTCCCC GGAGAGCACG 
GACTCCTCCC CAGAGCGTGA CCTCCTGCCC AAGACCACCA TGCCCCCAGC TGGCCCCCAG 
CACGACACCA GCCTGGCCAT GGCCATCATC CGGAGGGAGG TGTCTCGGAT CCACGGTGAA 
GATGTGGCAC AGCCCTCGCC TGGGACCGAG GGCCCCCAGG AGAAGCACTT GCTCCAGCAG 
GACGCAGACG AGGCCGCCGC AGTGCCCTGT GCCCTGGGAG CCCAGGCCCC GGGCGGGATG 
CCTGTGGGGA AGGAGGAAGG CCCCTCTCAG AACCCCCTGC TGCGGGCTAA GGCGCTGGTG 
AAGAGGGTCA CCTGGAACCT CCAGGAGGCA GAGTGCGGGG CCCCGGCCAA TGACAGAGGC 
CTGTGGACAC CACTTCACAG GCCACAAAAG CCCCGGGAAG GAGTCTGGGA ATCGGACGAC 
GTGGGCCCCA TGGCTGGGTT CCAGCAGGCA CCCTTCTCTG AGCCGCCGGC CCCTGCTTAT 
GCGCTTCCAG AGCCTGGCTT CCCCTACACT GACCCCTCTC AGGTACAGAG CCCCAGCCTG 
CCCCCTGGCC CAGCCCTGCC ATCGGGCATC CCGCCTTATG CACCTATCAG CCAGCCCGCG 
GTCCAGTTCC TCCTCCAGGG GAGCCTGCCC CTCCCGGGCT GCGGGGCGGC CCAGAGCATG 
GCCCCAGTGT CCACCACCCC GACTGCAGCC TCAGAGCCAG CTGGCCACGC TGCCGCCACC 
ACTGCTGCCA ACAACTCTGA GGAGAGGACG GCTCCCCCCA GGCCGACCCC AGAGAAGGCC 
AAGAATGAGG AGTACATGAA GAAGCTGCAC GTGCAGGAGC GGGCCGTGGA GGAGGTGAAG 
CTGGCCATCA AGCCCTTCTA CCAGAAGAGG GAGGTGACCA AGGAGGAGTA TAAGGACATC 
CTGCGCAAGG CGGTGCAGAA GATTTGCCAC AGCAAGAGCG GGGAGATCAA CCCCGTGAAG 
GTGGGCAACC TGGTGAAGGC CTACGTGGAC AAGTACCGGC ACATGCGCAG ACACCGGCGG 
GCCGAGGCCG GCGAGGAGCC GCCCGCCCAG AGCGCCGAGG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_005227257.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227314.1 Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

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CloneID	OBa34952
Clone ID Related Accession (Same CDS sequence)	XM_005227257.4 , XM_005227257.3
Accession Version	XM_005227257.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	PHD and RING finger domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227257.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCCTGGA CGAGCTTGTG GACCGAAGCC CGGGGCCCGA TGGCCACCCC 
AGACTCCGTC CTGCCGCCCT GGCCAGCGAT GCTGAAGGAA GCAGTGATGC CCACAGCGAC 
GCCTCTGAGG ACGACTCTGG CAGCGAGCAC AGCGATGAGG AGGATGCGGA CGGGGACCTG 
GAGGACGGAT CTGGTTCCGA GGACTCTGAG GACGATGGTG ACGACCTGGA CGCGTTGGCA 
GCGGCGGCGG ATTCTCAGGG AGCTCTGGAA GGCAGCGGTG CGTTCAATTC TGATGACGAC 
TCCGAGAGCT GCCCGATCTG TCTCAATGCT TTCAGGGAGC AGGCTGTGGG AACGCCCGAG 
AACTGCGCCC ACTACTTCTG CCTCGATTGC ATTGTGGAGT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTAT ATTTAAGTGT ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTGAAGA AGATTCCAGT AGAGAGTGCC CACCTTGGGG AGAACGAGAA TGAAGACCCG 
ACCTTCTGTG AGGTGTGTGG CCGGAGCGAC CGGGAGGACC GGCTGCTGCT CTGTGACGGC 
TGCGACGCTG GGTACCACAT GGAGTGCTTG GACCCCCCTC TCCAGGAGGT TCCAGTGGAT 
GAGTGGTTCT GTCCGGAATG TGCTACCCCC GGGGCTGCTT CTGCCGCTGA TGCGGGTCCT 
GTGAGCGAGG AAGAGGTCTC CCTGCTCTTG GCTGACGTGG TGCCCACCAC CAGCCGGCTT 
CGGCCCCGCA CGGGGAGGAC ACGGGCCATC GCCAGGACGA GGCAGAGCGA GAGGGTGCGA 
GCGACAGTGA ACCGGAACCG GATCTCCACG GCCAGGAGGT TCCAGCGTGT CCCCAGGTAC 
CTCATGTCTT CTCTGCTGGA TGAGACCATT GAAGCCGTGG CTGCCGGGCT CAGCACGGCT 
GTGTACCAGC GCCCCGGGCC GCGTGCCCCA GCCCGCCGCA GGAGGAAAGG AGGAAGACGG 
AAGAAAACGT CAGGAAGAAA GAAAGCCCCG TCCAGATTGT CTGCAAAGAG CAGGAGCTCA 
GGGGCAAGGT TGAAGAAACG CCCAGGTCGT GTGAAGAAGA GGAAAGCACA GAAGATAAAG 
AATGAAGCCA CGGCTCGTTC GCGCATCGCA CGGACGCTGG GCCTCTGCAG GCCGGCCTGT 
GGAGCCTGCA CCCCCTCCGT GTACAAGCCT GCAGACCCGT CTCTGGGGCT GATGCGAGCC 
GACATCGGGG CAGCCTCCCT CTCCCTGTTT GGAGACCCCT ATGAATTGGA TCCCTTTGAC 
AGTGAAGAGG CGTCTGCAAG TCCTGCTTCC CCTCTGAGCG CCAAGAGAAG GGTTCTTTCC 
CGATCAGCCC TGCGCTCCCA CCAGCCTGTA GCCAGACCCA TCTCCATGGG GCTCTCCAGG 
AGAGGTGCTC CTGCCGTGGC CCCCCAGCCG GAAGTGGACA CAGAGCCCAG CCCTGACCTG 
CTGGGGAGCA TCCTGTCTGG CCAGAGCCTT CTGATGATGA ACGGTGCCGA CATCATCATC 
CACCGCGACG GCTCCCTCAG CGCCAAGAGG GCAGTGCCAG TCTCTTTTCA GCAGAACTCA 
GCTGGTCCAT CCAGAGAGCC AGAAGGCGCC TGGGGGCGTC AGTCAGGAAG CGGGCCACAG 
AGCTCGGGGC CCAGCTGCCC GGGCAGGGCC GCCCCCGCCC CCACCCGTGT CCCTGCACTC 
ACATCGGGGG CAGCGGCAAA GCCGGACTCC TCGGTGACCC CTCGGTTGGG CCAGTCTCAG 
ACCTCGTCCA GCGAGAGCAG ACCTGGTATA CAGCAGAGTG AGGGTGGCCA GTGTAACGGT 
GACAGCAAGC ACTCTCTGCC TCTTGGGCCT GCGGCGCTGA AGGCCTCACA CAGCAGTTCT 
GACTCGCCCT CCAAGAGTAC AGTGCCGGGC CAGGCCCCGA GACCAGCTCC CAGGCGGACA 
GACATCTCCG AGCTCCCCAG GATACCAAAG ATCAGGAGAG ACAGCAGCCG GGGGGACGAG 
GGCCCTGCCA GTAGGCAGGG CGTCGAGCTC CCCAGCTCCT GCATCAGCCG GCTGACAGGC 
AGGGAGGGCC CTGGACAGCC TGGCTGCGGG GCCCGGGTGG AGGGTGAGCC CAGCAACCGG 
GGCGCACAGG AGCCCAGCAC GCACTCCGGG GGGGGCCCCC AGCCCCCCGG CCCGCTGGGG 
CCGGCCCGGG GGAAGGCTGT TGGCTCCACC TTTGAGAGTT TCAGGATCAA CATTCCTGGG 
AACACAGCCC ATTCTGGCAG ACTGCTCAGC CCTGGCTTCT GTAACACGTT CCGGCCGGTG 
GACAGCAAGG TGCAGAGGAG AGCGAGCCCC TCGCCCCTCT TCTCTGTCAG GAAGACGAAG 
CAGCTCAAGA GCGAGATCTA TGACCCCTTC AACCCCACGG GCTCCGACTC CAGCTCTGCC 
AACAGCAGCC CCGAGCGTCT GGGCCTCCTG CCCTCGGAGA TCACGCGGAC CATCTCTGTT 
GACAGCCCCA AGGCCCCGGT GCTGCCCCCC GTGCGCTGCG TCACCTCCTA CACGGTGCAG 
ACAGGGGCGG GGAGAGAGCT GGAGCCCCCC CAGGGGCCCT CCAGTCTGCC TGAGCTCCAG 
GATGAGGAGC CCTGCGCACA GGCTCAGCGG CCGTCCCCCC CGGAGCCCTG GGGCGATGAG 
GAGCTGCCTC CCCACAGCTC CTTCTTCGGC TCCGAGGGCC GGACAGTGAC CTGCGTGACT 
GTGGTGGAGC CCGACACGCC GCCCAGCCCA GACGCCCCGC CTGCCACCAC CCACAGGGTC 
GTGGAGCTGC GCTCCCTCTC CCGCTCCCGC TCCCGCTCCA GTTCCCGCAG CCGGAAGAAA 
GGCCCGCAGC CGAGGGCGGC CACCAGGGAG CACCGTGCCG GCCGCTCAGG CTCCCGCTCT 
TCCCGCTCAG GAGACCGGAG TTCCAGGTCT GCCTCGCCGC CGGCGGGCGA GGACCAGCCC 
AAGGGACACC GGCCCAAGGC CCGGGGCCGC AGGTCCTCCA GCGAGCACTC CAGCAGCCGC 
GAGCGTGCCA AGCGGAAGAA GGCGAAGGAC AAGAGCAGGG AGCGCGGCAG AGGCTCCTGG 
GGTCGCGGCC GTCGGAGGTC CCGCTCCCGC TCTGGGAGCC CTGGGAGCTC GTCCCACGAG 
CACCGCGAGG GCCGCAGGAA GAAGAGGCGA CGGTCAGGGT CCAGGTCTCG GGGGAGGGAG 
TGCTCGCCCC CTAGGCACCC CAGGGAGAGG AGGGGGCGGC CCCGAGACAG GCGCGGCTCC 
CGCGAGAGGC GGCGGCGAAG GTCCAGGTCC CCCAGCTCGG AACACCGGCC CCGGGCGCAC 
CGGCGGCCTC GGTCCCGGGA GAAGCGCTCG CGGCCCCGCC CCCACTCCCC TGAGCGGAAG 
TTGGCCCCGA AAGAGGCTTC TGCAGCACCC GCTCCCCAGG GGGAGCCCAG GCCAGACAGG 
GAGCCCCCAG CCAGGTCCCC GGCTAGACCC CCGTCCTCAG CGGGAGCAGA CGGCTTGCCA 
GAAGGGCCCG AAGTCCACAA GACTGCCCCG GTCCCGGAGG TGCTGGCCGC GTGTCCAGCG 
GAGGACGTGG ACTATGGCGA CTCTGTGGAG GCGGGGCACC TCTTTGAGGA CTTTTCCAGT 
GACACCATCT TCATGCAGCT TGATGATATG AGTTCCCCGC CCTCCCCGGA GAGCACGGAC 
TCCTCCCCAG AGCGTGACCT CCTGCCCAAG ACCACCATGC CCCCAGCTGG CCCCCAGCAC 
GACACCAGCC TGGCCATGGC CATCATCCGG AGGGAGGTGT CTCGGATCCA CGGTGAAGAT 
GTGGCACAGC CCTCGCCTGG GACCGAGGGC CCCCAGGAGA AGCACTTGCT CCAGCAGGAC 
GCAGACGAGG CCGCCGCAGT GCCCTGTGCC CTGGGAGCCC AGGCCCCGGG CGGGATGCCT 
GTGGGGAAGG AGGAAGGCCC CTCTCAGAAC CCCCTGCTGC GGGCTAAGGC GCTGGTGAAG 
AGGGTCACCT GGAACCTCCA GGAGGCAGAG TGCGGGGCCC CGGCCAATGA CAGAGGCCTG 
TGGACACCAC TTCACAGGCC ACAAAAGCCC CGGGAAGGAG TCTGGGAATC GGACGACGTG 
GGCCCCATGG CTGGGTTCCA GCAGGCACCC TTCTCTGAGC CGCCGGCCCC TGCTTATGCG 
CTTCCAGAGC CTGGCTTCCC CTACACTGAC CCCTCTCAGG TACAGAGCCC CAGCCTGCCC 
CCTGGCCCAG CCCTGCCATC GGGCATCCCG CCTTATGCAC CTATCAGCCA GCCCGCGGTC 
CAGTTCCTCC TCCAGGGGAG CCTGCCCCTC CCGGGCTGCG GGGCGGCCCA GAGCATGGCC 
CCAGTGTCCA CCACCCCGAC TGCAGCCTCA GAGCCAGCTG GCCACGCTGC CGCCACCACT 
GCTGCCAACA ACTCTGAGGA GAGGACGGCT CCCCCCAGGC CGACCCCAGA GAAGGCCAAG 
AATGAGGAGT ACATGAAGAA GCTGCACGTG CAGGAGCGGG CCGTGGAGGA GGTGAAGCTG 
GCCATCAAGC CCTTCTACCA GAAGAGGGAG GTGACCAAGG AGGAGTATAA GGACATCCTG 
CGCAAGGCGG TGCAGAAGAT TTGCCACAGC AAGAGCGGGG AGATCAACCC CGTGAAGGTG 
GGCAACCTGG TGAAGGCCTA CGTGGACAAG TACCGGCACA TGCGCAGACA CCGGCGGGCC 
GAGGCCGGCG AGGAGCCGCC CGCCCAGAGC GCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227314.1
CDS	152..4933
Translation	MDDDSLDELVDRSPGPDGHPRLRPAALASDAEGSSDAHSDASEDDSGSEHSDEEDADGDLEDGSGSEDSEDDGDDLDALAAAADSQGALEGSGAFNSDDDSESCPICLNAFREQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRAQFGGKILKKIPVESAHLGENENEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECATPGAASAADAGPVSEEEVSLLLADVVPTTSRLRPRTGRTRAIARTRQSERVRATVNRNRISTARRFQRVPRYLMSSLLDETIEAVAAGLSTAVYQRPGPRAPARRRRKGGRRKKTSGRKKAPSRLSAKSRSSGARLKKRPGRVKKRKAQKIKNEATARSRIARTLGLCRPACGACTPSVYKPADPSLGLMRADIGAASLSLFGDPYELDPFDSEEASASPASPLSAKRRVLSRSALRSHQPVARPISMGLSRRGAPAVAPQPEVDTEPSPDLLGSILSGQSLLMMNGADIIIHRDGSLSAKRAVPVSFQQNSAGPSREPEGAWGRQSGSGPQSSGPSCPGRAAPAPTRVPALTSGAAAKPDSSVTPRLGQSQTSSSESRPGIQQSEGGQCNGDSKHSLPLGPAALKASHSSSDSPSKSTVPGQAPRPAPRRTDISELPRIPKIRRDSSRGDEGPASRQGVELPSSCISRLTGREGPGQPGCGARVEGEPSNRGAQEPSTHSGGGPQPPGPLGPARGKAVGSTFESFRINIPGNTAHSGRLLSPGFCNTFRPVDSKVQRRASPSPLFSVRKTKQLKSEIYDPFNPTGSDSSSANSSPERLGLLPSEITRTISVDSPKAPVLPPVRCVTSYTVQTGAGRELEPPQGPSSLPELQDEEPCAQAQRPSPPEPWGDEELPPHSSFFGSEGRTVTCVTVVEPDTPPSPDAPPATTHRVVELRSLSRSRSRSSSRSRKKGPQPRAATREHRAGRSGSRSSRSGDRSSRSASPPAGEDQPKGHRPKARGRRSSSEHSSSRERAKRKKAKDKSRERGRGSWGRGRRRSRSRSGSPGSSSHEHREGRRKKRRRSGSRSRGRECSPPRHPRERRGRPRDRRGSRERRRRRSRSPSSEHRPRAHRRPRSREKRSRPRPHSPERKLAPKEASAAPAPQGEPRPDREPPARSPARPPSSAGADGLPEGPEVHKTAPVPEVLAACPAEDVDYGDSVEAGHLFEDFSSDTIFMQLDDMSSPPSPESTDSSPERDLLPKTTMPPAGPQHDTSLAMAIIRREVSRIHGEDVAQPSPGTEGPQEKHLLQQDADEAAAVPCALGAQAPGGMPVGKEEGPSQNPLLRAKALVKRVTWNLQEAECGAPANDRGLWTPLHRPQKPREGVWESDDVGPMAGFQQAPFSEPPAPAYALPEPGFPYTDPSQVQSPSLPPGPALPSGIPPYAPISQPAVQFLLQGSLPLPGCGAAQSMAPVSTTPTAASEPAGHAAATTAANNSEERTAPPRPTPEKAKNEEYMKKLHVQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEPPAQSAEG

Target ORF information:

RefSeq Version	XM_005227257.4
Organism	Bos taurus(cattle)
Definition	Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227257.4

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA CGAGCTTGTG GACCGAAGCC CGGGGCCCGA TGGCCACCCC 
AGACTCCGTC CTGCCGCCCT GGCCAGCGAT GCTGAAGGAA GCAGTGATGC CCACAGCGAC 
GCCTCTGAGG ACGACTCTGG CAGCGAGCAC AGCGATGAGG AGGATGCGGA CGGGGACCTG 
GAGGACGGAT CTGGTTCCGA GGACTCTGAG GACGATGGTG ACGACCTGGA CGCGTTGGCA 
GCGGCGGCGG ATTCTCAGGG AGCTCTGGAA GGCAGCGGTG CGTTCAATTC TGATGACGAC 
TCCGAGAGCT GCCCGATCTG TCTCAATGCT TTCAGGGAGC AGGCTGTGGG AACGCCCGAG 
AACTGCGCCC ACTACTTCTG CCTCGATTGC ATTGTGGAGT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTAT ATTTAAGTGT ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTGAAGA AGATTCCAGT AGAGAGTGCC CACCTTGGGG AGAACGAGAA TGAAGACCCG 
ACCTTCTGTG AGGTGTGTGG CCGGAGCGAC CGGGAGGACC GGCTGCTGCT CTGTGACGGC 
TGCGACGCTG GGTACCACAT GGAGTGCTTG GACCCCCCTC TCCAGGAGGT TCCAGTGGAT 
GAGTGGTTCT GTCCGGAATG TGCTACCCCC GGGGCTGCTT CTGCCGCTGA TGCGGGTCCT 
GTGAGCGAGG AAGAGGTCTC CCTGCTCTTG GCTGACGTGG TGCCCACCAC CAGCCGGCTT 
CGGCCCCGCA CGGGGAGGAC ACGGGCCATC GCCAGGACGA GGCAGAGCGA GAGGGTGCGA 
GCGACAGTGA ACCGGAACCG GATCTCCACG GCCAGGAGGT TCCAGCGTGT CCCCAGGTAC 
CTCATGTCTT CTCTGCTGGA TGAGACCATT GAAGCCGTGG CTGCCGGGCT CAGCACGGCT 
GTGTACCAGC GCCCCGGGCC GCGTGCCCCA GCCCGCCGCA GGAGGAAAGG AGGAAGACGG 
AAGAAAACGT CAGGAAGAAA GAAAGCCCCG TCCAGATTGT CTGCAAAGAG CAGGAGCTCA 
GGGGCAAGGT TGAAGAAACG CCCAGGTCGT GTGAAGAAGA GGAAAGCACA GAAGATAAAG 
AATGAAGCCA CGGCTCGTTC GCGCATCGCA CGGACGCTGG GCCTCTGCAG GCCGGCCTGT 
GGAGCCTGCA CCCCCTCCGT GTACAAGCCT GCAGACCCGT CTCTGGGGCT GATGCGAGCC 
GACATCGGGG CAGCCTCCCT CTCCCTGTTT GGAGACCCCT ATGAATTGGA TCCCTTTGAC 
AGTGAAGAGG CGTCTGCAAG TCCTGCTTCC CCTCTGAGCG CCAAGAGAAG GGTTCTTTCC 
CGATCAGCCC TGCGCTCCCA CCAGCCTGTA GCCAGACCCA TCTCCATGGG GCTCTCCAGG 
AGAGGTGCTC CTGCCGTGGC CCCCCAGCCG GAAGTGGACA CAGAGCCCAG CCCTGACCTG 
CTGGGGAGCA TCCTGTCTGG CCAGAGCCTT CTGATGATGA ACGGTGCCGA CATCATCATC 
CACCGCGACG GCTCCCTCAG CGCCAAGAGG GCAGTGCCAG TCTCTTTTCA GCAGAACTCA 
GCTGGTCCAT CCAGAGAGCC AGAAGGCGCC TGGGGGCGTC AGTCAGGAAG CGGGCCACAG 
AGCTCGGGGC CCAGCTGCCC GGGCAGGGCC GCCCCCGCCC CCACCCGTGT CCCTGCACTC 
ACATCGGGGG CAGCGGCAAA GCCGGACTCC TCGGTGACCC CTCGGTTGGG CCAGTCTCAG 
ACCTCGTCCA GCGAGAGCAG ACCTGGTATA CAGCAGAGTG AGGGTGGCCA GTGTAACGGT 
GACAGCAAGC ACTCTCTGCC TCTTGGGCCT GCGGCGCTGA AGGCCTCACA CAGCAGTTCT 
GACTCGCCCT CCAAGAGTAC AGTGCCGGGC CAGGCCCCGA GACCAGCTCC CAGGCGGACA 
GACATCTCCG AGCTCCCCAG GATACCAAAG ATCAGGAGAG ACAGCAGCCG GGGGGACGAG 
GGCCCTGCCA GTAGGCAGGG CGTCGAGCTC CCCAGCTCCT GCATCAGCCG GCTGACAGGC 
AGGGAGGGCC CTGGACAGCC TGGCTGCGGG GCCCGGGTGG AGGGTGAGCC CAGCAACCGG 
GGCGCACAGG AGCCCAGCAC GCACTCCGGG GGGGGCCCCC AGCCCCCCGG CCCGCTGGGG 
CCGGCCCGGG GGAAGGCTGT TGGCTCCACC TTTGAGAGTT TCAGGATCAA CATTCCTGGG 
AACACAGCCC ATTCTGGCAG ACTGCTCAGC CCTGGCTTCT GTAACACGTT CCGGCCGGTG 
GACAGCAAGG TGCAGAGGAG AGCGAGCCCC TCGCCCCTCT TCTCTGTCAG GAAGACGAAG 
CAGCTCAAGA GCGAGATCTA TGACCCCTTC AACCCCACGG GCTCCGACTC CAGCTCTGCC 
AACAGCAGCC CCGAGCGTCT GGGCCTCCTG CCCTCGGAGA TCACGCGGAC CATCTCTGTT 
GACAGCCCCA AGGCCCCGGT GCTGCCCCCC GTGCGCTGCG TCACCTCCTA CACGGTGCAG 
ACAGGGGCGG GGAGAGAGCT GGAGCCCCCC CAGGGGCCCT CCAGTCTGCC TGAGCTCCAG 
GATGAGGAGC CCTGCGCACA GGCTCAGCGG CCGTCCCCCC CGGAGCCCTG GGGCGATGAG 
GAGCTGCCTC CCCACAGCTC CTTCTTCGGC TCCGAGGGCC GGACAGTGAC CTGCGTGACT 
GTGGTGGAGC CCGACACGCC GCCCAGCCCA GACGCCCCGC CTGCCACCAC CCACAGGGTC 
GTGGAGCTGC GCTCCCTCTC CCGCTCCCGC TCCCGCTCCA GTTCCCGCAG CCGGAAGAAA 
GGCCCGCAGC CGAGGGCGGC CACCAGGGAG CACCGTGCCG GCCGCTCAGG CTCCCGCTCT 
TCCCGCTCAG GAGACCGGAG TTCCAGGTCT GCCTCGCCGC CGGCGGGCGA GGACCAGCCC 
AAGGGACACC GGCCCAAGGC CCGGGGCCGC AGGTCCTCCA GCGAGCACTC CAGCAGCCGC 
GAGCGTGCCA AGCGGAAGAA GGCGAAGGAC AAGAGCAGGG AGCGCGGCAG AGGCTCCTGG 
GGTCGCGGCC GTCGGAGGTC CCGCTCCCGC TCTGGGAGCC CTGGGAGCTC GTCCCACGAG 
CACCGCGAGG GCCGCAGGAA GAAGAGGCGA CGGTCAGGGT CCAGGTCTCG GGGGAGGGAG 
TGCTCGCCCC CTAGGCACCC CAGGGAGAGG AGGGGGCGGC CCCGAGACAG GCGCGGCTCC 
CGCGAGAGGC GGCGGCGAAG GTCCAGGTCC CCCAGCTCGG AACACCGGCC CCGGGCGCAC 
CGGCGGCCTC GGTCCCGGGA GAAGCGCTCG CGGCCCCGCC CCCACTCCCC TGAGCGGAAG 
TTGGCCCCGA AAGAGGCTTC TGCAGCACCC GCTCCCCAGG GGGAGCCCAG GCCAGACAGG 
GAGCCCCCAG CCAGGTCCCC GGCTAGACCC CCGTCCTCAG CGGGAGCAGA CGGCTTGCCA 
GAAGGGCCCG AAGTCCACAA GACTGCCCCG GTCCCGGAGG TGCTGGCCGC GTGTCCAGCG 
GAGGACGTGG ACTATGGCGA CTCTGTGGAG GCGGGGCACC TCTTTGAGGA CTTTTCCAGT 
GACACCATCT TCATGCAGCT TGATGATATG AGTTCCCCGC CCTCCCCGGA GAGCACGGAC 
TCCTCCCCAG AGCGTGACCT CCTGCCCAAG ACCACCATGC CCCCAGCTGG CCCCCAGCAC 
GACACCAGCC TGGCCATGGC CATCATCCGG AGGGAGGTGT CTCGGATCCA CGGTGAAGAT 
GTGGCACAGC CCTCGCCTGG GACCGAGGGC CCCCAGGAGA AGCACTTGCT CCAGCAGGAC 
GCAGACGAGG CCGCCGCAGT GCCCTGTGCC CTGGGAGCCC AGGCCCCGGG CGGGATGCCT 
GTGGGGAAGG AGGAAGGCCC CTCTCAGAAC CCCCTGCTGC GGGCTAAGGC GCTGGTGAAG 
AGGGTCACCT GGAACCTCCA GGAGGCAGAG TGCGGGGCCC CGGCCAATGA CAGAGGCCTG 
TGGACACCAC TTCACAGGCC ACAAAAGCCC CGGGAAGGAG TCTGGGAATC GGACGACGTG 
GGCCCCATGG CTGGGTTCCA GCAGGCACCC TTCTCTGAGC CGCCGGCCCC TGCTTATGCG 
CTTCCAGAGC CTGGCTTCCC CTACACTGAC CCCTCTCAGG TACAGAGCCC CAGCCTGCCC 
CCTGGCCCAG CCCTGCCATC GGGCATCCCG CCTTATGCAC CTATCAGCCA GCCCGCGGTC 
CAGTTCCTCC TCCAGGGGAG CCTGCCCCTC CCGGGCTGCG GGGCGGCCCA GAGCATGGCC 
CCAGTGTCCA CCACCCCGAC TGCAGCCTCA GAGCCAGCTG GCCACGCTGC CGCCACCACT 
GCTGCCAACA ACTCTGAGGA GAGGACGGCT CCCCCCAGGC CGACCCCAGA GAAGGCCAAG 
AATGAGGAGT ACATGAAGAA GCTGCACGTG CAGGAGCGGG CCGTGGAGGA GGTGAAGCTG 
GCCATCAAGC CCTTCTACCA GAAGAGGGAG GTGACCAAGG AGGAGTATAA GGACATCCTG 
CGCAAGGCGG TGCAGAAGAT TTGCCACAGC AAGAGCGGGG AGATCAACCC CGTGAAGGTG 
GGCAACCTGG TGAAGGCCTA CGTGGACAAG TACCGGCACA TGCGCAGACA CCGGCGGGCC 
GAGGCCGGCG AGGAGCCGCC CGCCCAGAGC GCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_005227257.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227314.1 Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

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CloneID	OBa34952
Clone ID Related Accession (Same CDS sequence)	XM_005227257.4 , XM_005227257.3
Accession Version	XM_005227257.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	PHD and RING finger domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCCTGGA CGAGCTTGTG GACCGAAGCC CGGGGCCCGA TGGCCACCCC 
AGACTCCGTC CTGCCGCCCT GGCCAGCGAT GCTGAAGGAA GCAGTGATGC CCACAGCGAC 
GCCTCTGAGG ACGACTCTGG CAGCGAGCAC AGCGATGAGG AGGATGCGGA CGGGGACCTG 
GAGGACGGAT CTGGTTCCGA GGACTCTGAG GACGATGGTG ACGACCTGGA CGCGTTGGCA 
GCGGCGGCGG ATTCTCAGGG AGCTCTGGAA GGCAGCGGTG CGTTCAATTC TGATGACGAC 
TCCGAGAGCT GCCCGATCTG TCTCAATGCT TTCAGGGAGC AGGCTGTGGG AACGCCCGAG 
AACTGCGCCC ACTACTTCTG CCTCGATTGC ATTGTGGAGT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTAT ATTTAAGTGT ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTGAAGA AGATTCCAGT AGAGAGTGCC CACCTTGGGG AGAACGAGAA TGAAGACCCG 
ACCTTCTGTG AGGTGTGTGG CCGGAGCGAC CGGGAGGACC GGCTGCTGCT CTGTGACGGC 
TGCGACGCTG GGTACCACAT GGAGTGCTTG GACCCCCCTC TCCAGGAGGT TCCAGTGGAT 
GAGTGGTTCT GTCCGGAATG TGCTACCCCC GGGGCTGCTT CTGCCGCTGA TGCGGGTCCT 
GTGAGCGAGG AAGAGGTCTC CCTGCTCTTG GCTGACGTGG TGCCCACCAC CAGCCGGCTT 
CGGCCCCGCA CGGGGAGGAC ACGGGCCATC GCCAGGACGA GGCAGAGCGA GAGGGTGCGA 
GCGACAGTGA ACCGGAACCG GATCTCCACG GCCAGGAGGT TCCAGCGTGT CCCCAGGTAC 
CTCATGTCTT CTCTGCTGGA TGAGACCATT GAAGCCGTGG CTGCCGGGCT CAGCACGGCT 
GTGTACCAGC GCCCCGGGCC GCGTGCCCCA GCCCGCCGCA GGAGGAAAGG AGGAAGACGG 
AAGAAAACGT CAGGAAGAAA GAAAGCCCCG TCCAGATTGT CTGCAAAGAG CAGGAGCTCA 
GGGGCAAGGT TGAAGAAACG CCCAGGTCGT GTGAAGAAGA GGAAAGCACA GAAGATAAAG 
AATGAAGCCA CGGCTCGTTC GCGCATCGCA CGGACGCTGG GCCTCTGCAG GCCGGCCTGT 
GGAGCCTGCA CCCCCTCCGT GTACAAGCCT GCAGACCCGT CTCTGGGGCT GATGCGAGCC 
GACATCGGGG CAGCCTCCCT CTCCCTGTTT GGAGACCCCT ATGAATTGGA TCCCTTTGAC 
AGTGAAGAGG CGTCTGCAAG TCCTGCTTCC CCTCTGAGCG CCAAGAGAAG GGTTCTTTCC 
CGATCAGCCC TGCGCTCCCA CCAGCCTGTA GCCAGACCCA TCTCCATGGG GCTCTCCAGG 
AGAGGTGCTC CTGCCGTGGC CCCCCAGCCG GAAGTGGACA CAGAGCCCAG CCCTGACCTG 
CTGGGGAGCA TCCTGTCTGG CCAGAGCCTT CTGATGATGA ACGGTGCCGA CATCATCATC 
CACCGCGACG GCTCCCTCAG CGCCAAGAGG GCAGTGCCAG TCTCTTTTCA GCAGAACTCA 
GCTGGTCCAT CCAGAGAGCC AGAAGGCGCC TGGGGGCGTC AGTCAGGAAG CGGGCCACAG 
AGCTCGGGGC CCAGCTGCCC GGGCAGGGCC GCCCCCGCCC CCACCCGTGT CCCTGCACTC 
ACATCGGGGG CAGCGGCAAA GCCGGACTCC TCGGTGACCC CTCGGTTGGG CCAGTCTCAG 
ACCTCGTCCA GCGAGAGCAG ACCTGGTATA CAGCAGAGTG AGGGTGGCCA GTGTAACGGT 
GACAGCAAGC ACTCTCTGCC TCTTGGGCCT GCGGCGCTGA AGGCCTCACA CAGCAGTTCT 
GACTCGCCCT CCAAGAGTAC AGTGCCGGGC CAGGCCCCGA GACCAGCTCC CAGGCGGACA 
GACATCTCCG AGCTCCCCAG GATACCAAAG ATCAGGAGAG ACAGCAGCCG GGGGGACGAG 
GGCCCTGCCA GTAGGCAGGG CGTCGAGCTC CCCAGCTCCT GCATCAGCCG GCTGACAGGC 
AGGGAGGGCC CTGGACAGCC TGGCTGCGGG GCCCGGGTGG AGGGTGAGCC CAGCAACCGG 
GGCGCACAGG AGCCCAGCAC GCACTCCGGG GGGGGCCCCC AGCCCCCCGG CCCGCTGGGG 
CCGGCCCGGG GGAAGGCTGT TGGCTCCACC TTTGAGAGTT TCAGGATCAA CATTCCTGGG 
AACACAGCCC ATTCTGGCAG ACTGCTCAGC CCTGGCTTCT GTAACACGTT CCGGCCGGTG 
GACAGCAAGG TGCAGAGGAG AGCGAGCCCC TCGCCCCTCT TCTCTGTCAG GAAGACGAAG 
CAGCTCAAGA GCGAGATCTA TGACCCCTTC AACCCCACGG GCTCCGACTC CAGCTCTGCC 
AACAGCAGCC CCGAGCGTCT GGGCCTCCTG CCCTCGGAGA TCACGCGGAC CATCTCTGTT 
GACAGCCCCA AGGCCCCGGT GCTGCCCCCC GTGCGCTGCG TCACCTCCTA CACGGTGCAG 
ACAGGGGCGG GGAGAGAGCT GGAGCCCCCC CAGGGGCCCT CCAGTCTGCC TGAGCTCCAG 
GATGAGGAGC CCTGCGCACA GGCTCAGCGG CCGTCCCCCC CGGAGCCCTG GGGCGATGAG 
GAGCTGCCTC CCCACAGCTC CTTCTTCGGC TCCGAGGGCC GGACAGTGAC CTGCGTGACT 
GTGGTGGAGC CCGACACGCC GCCCAGCCCA GACGCCCCGC CTGCCACCAC CCACAGGGTC 
GTGGAGCTGC GCTCCCTCTC CCGCTCCCGC TCCCGCTCCA GTTCCCGCAG CCGGAAGAAA 
GGCCCGCAGC CGAGGGCGGC CACCAGGGAG CACCGTGCCG GCCGCTCAGG CTCCCGCTCT 
TCCCGCTCAG GAGACCGGAG TTCCAGGTCT GCCTCGCCGC CGGCGGGCGA GGACCAGCCC 
AAGGGACACC GGCCCAAGGC CCGGGGCCGC AGGTCCTCCA GCGAGCACTC CAGCAGCCGC 
GAGCGTGCCA AGCGGAAGAA GGCGAAGGAC AAGAGCAGGG AGCGCGGCAG AGGCTCCTGG 
GGTCGCGGCC GTCGGAGGTC CCGCTCCCGC TCTGGGAGCC CTGGGAGCTC GTCCCACGAG 
CACCGCGAGG GCCGCAGGAA GAAGAGGCGA CGGTCAGGGT CCAGGTCTCG GGGGAGGGAG 
TGCTCGCCCC CTAGGCACCC CAGGGAGAGG AGGGGGCGGC CCCGAGACAG GCGCGGCTCC 
CGCGAGAGGC GGCGGCGAAG GTCCAGGTCC CCCAGCTCGG AACACCGGCC CCGGGCGCAC 
CGGCGGCCTC GGTCCCGGGA GAAGCGCTCG CGGCCCCGCC CCCACTCCCC TGAGCGGAAG 
TTGGCCCCGA AAGAGGCTTC TGCAGCACCC GCTCCCCAGG GGGAGCCCAG GCCAGACAGG 
GAGCCCCCAG CCAGGTCCCC GGCTAGACCC CCGTCCTCAG CGGGAGCAGA CGGCTTGCCA 
GAAGGGCCCG AAGTCCACAA GACTGCCCCG GTCCCGGAGG TGCTGGCCGC GTGTCCAGCG 
GAGGACGTGG ACTATGGCGA CTCTGTGGAG GCGGGGCACC TCTTTGAGGA CTTTTCCAGT 
GACACCATCT TCATGCAGCT TGATGATATG AGTTCCCCGC CCTCCCCGGA GAGCACGGAC 
TCCTCCCCAG AGCGTGACCT CCTGCCCAAG ACCACCATGC CCCCAGCTGG CCCCCAGCAC 
GACACCAGCC TGGCCATGGC CATCATCCGG AGGGAGGTGT CTCGGATCCA CGGTGAAGAT 
GTGGCACAGC CCTCGCCTGG GACCGAGGGC CCCCAGGAGA AGCACTTGCT CCAGCAGGAC 
GCAGACGAGG CCGCCGCAGT GCCCTGTGCC CTGGGAGCCC AGGCCCCGGG CGGGATGCCT 
GTGGGGAAGG AGGAAGGCCC CTCTCAGAAC CCCCTGCTGC GGGCTAAGGC GCTGGTGAAG 
AGGGTCACCT GGAACCTCCA GGAGGCAGAG TGCGGGGCCC CGGCCAATGA CAGAGGCCTG 
TGGACACCAC TTCACAGGCC ACAAAAGCCC CGGGAAGGAG TCTGGGAATC GGACGACGTG 
GGCCCCATGG CTGGGTTCCA GCAGGCACCC TTCTCTGAGC CGCCGGCCCC TGCTTATGCG 
CTTCCAGAGC CTGGCTTCCC CTACACTGAC CCCTCTCAGG TACAGAGCCC CAGCCTGCCC 
CCTGGCCCAG CCCTGCCATC GGGCATCCCG CCTTATGCAC CTATCAGCCA GCCCGCGGTC 
CAGTTCCTCC TCCAGGGGAG CCTGCCCCTC CCGGGCTGCG GGGCGGCCCA GAGCATGGCC 
CCAGTGTCCA CCACCCCGAC TGCAGCCTCA GAGCCAGCTG GCCACGCTGC CGCCACCACT 
GCTGCCAACA ACTCTGAGGA GAGGACGGCT CCCCCCAGGC CGACCCCAGA GAAGGCCAAG 
AATGAGGAGT ACATGAAGAA GCTGCACGTG CAGGAGCGGG CCGTGGAGGA GGTGAAGCTG 
GCCATCAAGC CCTTCTACCA GAAGAGGGAG GTGACCAAGG AGGAGTATAA GGACATCCTG 
CGCAAGGCGG TGCAGAAGAT TTGCCACAGC AAGAGCGGGG AGATCAACCC CGTGAAGGTG 
GGCAACCTGG TGAAGGCCTA CGTGGACAAG TACCGGCACA TGCGCAGACA CCGGCGGGCC 
GAGGCCGGCG AGGAGCCGCC CGCCCAGAGC GCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227314.1
CDS	147..4928
Translation	MDDDSLDELVDRSPGPDGHPRLRPAALASDAEGSSDAHSDASEDDSGSEHSDEEDADGDLEDGSGSEDSEDDGDDLDALAAAADSQGALEGSGAFNSDDDSESCPICLNAFREQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRAQFGGKILKKIPVESAHLGENENEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECATPGAASAADAGPVSEEEVSLLLADVVPTTSRLRPRTGRTRAIARTRQSERVRATVNRNRISTARRFQRVPRYLMSSLLDETIEAVAAGLSTAVYQRPGPRAPARRRRKGGRRKKTSGRKKAPSRLSAKSRSSGARLKKRPGRVKKRKAQKIKNEATARSRIARTLGLCRPACGACTPSVYKPADPSLGLMRADIGAASLSLFGDPYELDPFDSEEASASPASPLSAKRRVLSRSALRSHQPVARPISMGLSRRGAPAVAPQPEVDTEPSPDLLGSILSGQSLLMMNGADIIIHRDGSLSAKRAVPVSFQQNSAGPSREPEGAWGRQSGSGPQSSGPSCPGRAAPAPTRVPALTSGAAAKPDSSVTPRLGQSQTSSSESRPGIQQSEGGQCNGDSKHSLPLGPAALKASHSSSDSPSKSTVPGQAPRPAPRRTDISELPRIPKIRRDSSRGDEGPASRQGVELPSSCISRLTGREGPGQPGCGARVEGEPSNRGAQEPSTHSGGGPQPPGPLGPARGKAVGSTFESFRINIPGNTAHSGRLLSPGFCNTFRPVDSKVQRRASPSPLFSVRKTKQLKSEIYDPFNPTGSDSSSANSSPERLGLLPSEITRTISVDSPKAPVLPPVRCVTSYTVQTGAGRELEPPQGPSSLPELQDEEPCAQAQRPSPPEPWGDEELPPHSSFFGSEGRTVTCVTVVEPDTPPSPDAPPATTHRVVELRSLSRSRSRSSSRSRKKGPQPRAATREHRAGRSGSRSSRSGDRSSRSASPPAGEDQPKGHRPKARGRRSSSEHSSSRERAKRKKAKDKSRERGRGSWGRGRRRSRSRSGSPGSSSHEHREGRRKKRRRSGSRSRGRECSPPRHPRERRGRPRDRRGSRERRRRRSRSPSSEHRPRAHRRPRSREKRSRPRPHSPERKLAPKEASAAPAPQGEPRPDREPPARSPARPPSSAGADGLPEGPEVHKTAPVPEVLAACPAEDVDYGDSVEAGHLFEDFSSDTIFMQLDDMSSPPSPESTDSSPERDLLPKTTMPPAGPQHDTSLAMAIIRREVSRIHGEDVAQPSPGTEGPQEKHLLQQDADEAAAVPCALGAQAPGGMPVGKEEGPSQNPLLRAKALVKRVTWNLQEAECGAPANDRGLWTPLHRPQKPREGVWESDDVGPMAGFQQAPFSEPPAPAYALPEPGFPYTDPSQVQSPSLPPGPALPSGIPPYAPISQPAVQFLLQGSLPLPGCGAAQSMAPVSTTPTAASEPAGHAAATTAANNSEERTAPPRPTPEKAKNEEYMKKLHVQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEPPAQSAEG

Target ORF information:

RefSeq Version	XM_005227257.3
Organism	Bos taurus(cattle)
Definition	Bos taurus PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227257.3

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA CGAGCTTGTG GACCGAAGCC CGGGGCCCGA TGGCCACCCC 
AGACTCCGTC CTGCCGCCCT GGCCAGCGAT GCTGAAGGAA GCAGTGATGC CCACAGCGAC 
GCCTCTGAGG ACGACTCTGG CAGCGAGCAC AGCGATGAGG AGGATGCGGA CGGGGACCTG 
GAGGACGGAT CTGGTTCCGA GGACTCTGAG GACGATGGTG ACGACCTGGA CGCGTTGGCA 
GCGGCGGCGG ATTCTCAGGG AGCTCTGGAA GGCAGCGGTG CGTTCAATTC TGATGACGAC 
TCCGAGAGCT GCCCGATCTG TCTCAATGCT TTCAGGGAGC AGGCTGTGGG AACGCCCGAG 
AACTGCGCCC ACTACTTCTG CCTCGATTGC ATTGTGGAGT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTAT ATTTAAGTGT ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTGAAGA AGATTCCAGT AGAGAGTGCC CACCTTGGGG AGAACGAGAA TGAAGACCCG 
ACCTTCTGTG AGGTGTGTGG CCGGAGCGAC CGGGAGGACC GGCTGCTGCT CTGTGACGGC 
TGCGACGCTG GGTACCACAT GGAGTGCTTG GACCCCCCTC TCCAGGAGGT TCCAGTGGAT 
GAGTGGTTCT GTCCGGAATG TGCTACCCCC GGGGCTGCTT CTGCCGCTGA TGCGGGTCCT 
GTGAGCGAGG AAGAGGTCTC CCTGCTCTTG GCTGACGTGG TGCCCACCAC CAGCCGGCTT 
CGGCCCCGCA CGGGGAGGAC ACGGGCCATC GCCAGGACGA GGCAGAGCGA GAGGGTGCGA 
GCGACAGTGA ACCGGAACCG GATCTCCACG GCCAGGAGGT TCCAGCGTGT CCCCAGGTAC 
CTCATGTCTT CTCTGCTGGA TGAGACCATT GAAGCCGTGG CTGCCGGGCT CAGCACGGCT 
GTGTACCAGC GCCCCGGGCC GCGTGCCCCA GCCCGCCGCA GGAGGAAAGG AGGAAGACGG 
AAGAAAACGT CAGGAAGAAA GAAAGCCCCG TCCAGATTGT CTGCAAAGAG CAGGAGCTCA 
GGGGCAAGGT TGAAGAAACG CCCAGGTCGT GTGAAGAAGA GGAAAGCACA GAAGATAAAG 
AATGAAGCCA CGGCTCGTTC GCGCATCGCA CGGACGCTGG GCCTCTGCAG GCCGGCCTGT 
GGAGCCTGCA CCCCCTCCGT GTACAAGCCT GCAGACCCGT CTCTGGGGCT GATGCGAGCC 
GACATCGGGG CAGCCTCCCT CTCCCTGTTT GGAGACCCCT ATGAATTGGA TCCCTTTGAC 
AGTGAAGAGG CGTCTGCAAG TCCTGCTTCC CCTCTGAGCG CCAAGAGAAG GGTTCTTTCC 
CGATCAGCCC TGCGCTCCCA CCAGCCTGTA GCCAGACCCA TCTCCATGGG GCTCTCCAGG 
AGAGGTGCTC CTGCCGTGGC CCCCCAGCCG GAAGTGGACA CAGAGCCCAG CCCTGACCTG 
CTGGGGAGCA TCCTGTCTGG CCAGAGCCTT CTGATGATGA ACGGTGCCGA CATCATCATC 
CACCGCGACG GCTCCCTCAG CGCCAAGAGG GCAGTGCCAG TCTCTTTTCA GCAGAACTCA 
GCTGGTCCAT CCAGAGAGCC AGAAGGCGCC TGGGGGCGTC AGTCAGGAAG CGGGCCACAG 
AGCTCGGGGC CCAGCTGCCC GGGCAGGGCC GCCCCCGCCC CCACCCGTGT CCCTGCACTC 
ACATCGGGGG CAGCGGCAAA GCCGGACTCC TCGGTGACCC CTCGGTTGGG CCAGTCTCAG 
ACCTCGTCCA GCGAGAGCAG ACCTGGTATA CAGCAGAGTG AGGGTGGCCA GTGTAACGGT 
GACAGCAAGC ACTCTCTGCC TCTTGGGCCT GCGGCGCTGA AGGCCTCACA CAGCAGTTCT 
GACTCGCCCT CCAAGAGTAC AGTGCCGGGC CAGGCCCCGA GACCAGCTCC CAGGCGGACA 
GACATCTCCG AGCTCCCCAG GATACCAAAG ATCAGGAGAG ACAGCAGCCG GGGGGACGAG 
GGCCCTGCCA GTAGGCAGGG CGTCGAGCTC CCCAGCTCCT GCATCAGCCG GCTGACAGGC 
AGGGAGGGCC CTGGACAGCC TGGCTGCGGG GCCCGGGTGG AGGGTGAGCC CAGCAACCGG 
GGCGCACAGG AGCCCAGCAC GCACTCCGGG GGGGGCCCCC AGCCCCCCGG CCCGCTGGGG 
CCGGCCCGGG GGAAGGCTGT TGGCTCCACC TTTGAGAGTT TCAGGATCAA CATTCCTGGG 
AACACAGCCC ATTCTGGCAG ACTGCTCAGC CCTGGCTTCT GTAACACGTT CCGGCCGGTG 
GACAGCAAGG TGCAGAGGAG AGCGAGCCCC TCGCCCCTCT TCTCTGTCAG GAAGACGAAG 
CAGCTCAAGA GCGAGATCTA TGACCCCTTC AACCCCACGG GCTCCGACTC CAGCTCTGCC 
AACAGCAGCC CCGAGCGTCT GGGCCTCCTG CCCTCGGAGA TCACGCGGAC CATCTCTGTT 
GACAGCCCCA AGGCCCCGGT GCTGCCCCCC GTGCGCTGCG TCACCTCCTA CACGGTGCAG 
ACAGGGGCGG GGAGAGAGCT GGAGCCCCCC CAGGGGCCCT CCAGTCTGCC TGAGCTCCAG 
GATGAGGAGC CCTGCGCACA GGCTCAGCGG CCGTCCCCCC CGGAGCCCTG GGGCGATGAG 
GAGCTGCCTC CCCACAGCTC CTTCTTCGGC TCCGAGGGCC GGACAGTGAC CTGCGTGACT 
GTGGTGGAGC CCGACACGCC GCCCAGCCCA GACGCCCCGC CTGCCACCAC CCACAGGGTC 
GTGGAGCTGC GCTCCCTCTC CCGCTCCCGC TCCCGCTCCA GTTCCCGCAG CCGGAAGAAA 
GGCCCGCAGC CGAGGGCGGC CACCAGGGAG CACCGTGCCG GCCGCTCAGG CTCCCGCTCT 
TCCCGCTCAG GAGACCGGAG TTCCAGGTCT GCCTCGCCGC CGGCGGGCGA GGACCAGCCC 
AAGGGACACC GGCCCAAGGC CCGGGGCCGC AGGTCCTCCA GCGAGCACTC CAGCAGCCGC 
GAGCGTGCCA AGCGGAAGAA GGCGAAGGAC AAGAGCAGGG AGCGCGGCAG AGGCTCCTGG 
GGTCGCGGCC GTCGGAGGTC CCGCTCCCGC TCTGGGAGCC CTGGGAGCTC GTCCCACGAG 
CACCGCGAGG GCCGCAGGAA GAAGAGGCGA CGGTCAGGGT CCAGGTCTCG GGGGAGGGAG 
TGCTCGCCCC CTAGGCACCC CAGGGAGAGG AGGGGGCGGC CCCGAGACAG GCGCGGCTCC 
CGCGAGAGGC GGCGGCGAAG GTCCAGGTCC CCCAGCTCGG AACACCGGCC CCGGGCGCAC 
CGGCGGCCTC GGTCCCGGGA GAAGCGCTCG CGGCCCCGCC CCCACTCCCC TGAGCGGAAG 
TTGGCCCCGA AAGAGGCTTC TGCAGCACCC GCTCCCCAGG GGGAGCCCAG GCCAGACAGG 
GAGCCCCCAG CCAGGTCCCC GGCTAGACCC CCGTCCTCAG CGGGAGCAGA CGGCTTGCCA 
GAAGGGCCCG AAGTCCACAA GACTGCCCCG GTCCCGGAGG TGCTGGCCGC GTGTCCAGCG 
GAGGACGTGG ACTATGGCGA CTCTGTGGAG GCGGGGCACC TCTTTGAGGA CTTTTCCAGT 
GACACCATCT TCATGCAGCT TGATGATATG AGTTCCCCGC CCTCCCCGGA GAGCACGGAC 
TCCTCCCCAG AGCGTGACCT CCTGCCCAAG ACCACCATGC CCCCAGCTGG CCCCCAGCAC 
GACACCAGCC TGGCCATGGC CATCATCCGG AGGGAGGTGT CTCGGATCCA CGGTGAAGAT 
GTGGCACAGC CCTCGCCTGG GACCGAGGGC CCCCAGGAGA AGCACTTGCT CCAGCAGGAC 
GCAGACGAGG CCGCCGCAGT GCCCTGTGCC CTGGGAGCCC AGGCCCCGGG CGGGATGCCT 
GTGGGGAAGG AGGAAGGCCC CTCTCAGAAC CCCCTGCTGC GGGCTAAGGC GCTGGTGAAG 
AGGGTCACCT GGAACCTCCA GGAGGCAGAG TGCGGGGCCC CGGCCAATGA CAGAGGCCTG 
TGGACACCAC TTCACAGGCC ACAAAAGCCC CGGGAAGGAG TCTGGGAATC GGACGACGTG 
GGCCCCATGG CTGGGTTCCA GCAGGCACCC TTCTCTGAGC CGCCGGCCCC TGCTTATGCG 
CTTCCAGAGC CTGGCTTCCC CTACACTGAC CCCTCTCAGG TACAGAGCCC CAGCCTGCCC 
CCTGGCCCAG CCCTGCCATC GGGCATCCCG CCTTATGCAC CTATCAGCCA GCCCGCGGTC 
CAGTTCCTCC TCCAGGGGAG CCTGCCCCTC CCGGGCTGCG GGGCGGCCCA GAGCATGGCC 
CCAGTGTCCA CCACCCCGAC TGCAGCCTCA GAGCCAGCTG GCCACGCTGC CGCCACCACT 
GCTGCCAACA ACTCTGAGGA GAGGACGGCT CCCCCCAGGC CGACCCCAGA GAAGGCCAAG 
AATGAGGAGT ACATGAAGAA GCTGCACGTG CAGGAGCGGG CCGTGGAGGA GGTGAAGCTG 
GCCATCAAGC CCTTCTACCA GAAGAGGGAG GTGACCAAGG AGGAGTATAA GGACATCCTG 
CGCAAGGCGG TGCAGAAGAT TTGCCACAGC AAGAGCGGGG AGATCAACCC CGTGAAGGTG 
GGCAACCTGG TGAAGGCCTA CGTGGACAAG TACCGGCACA TGCGCAGACA CCGGCGGGCC 
GAGGCCGGCG AGGAGCCGCC CGCCCAGAGC GCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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PHRF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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