MIB2 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	MIB2
Entrez Gene ID	104995933
Full Name	mindbomb E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010849997.1	XP_010848299.1	E3 ubiquitin-protein ligase MIB2

Pathways from BioSystems
Gene Ontology

The following MIB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi72259	XM_010849997.1 Latest version!	Bison bison bison mindbomb E3 ubiquitin protein ligase 2 (MIB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIB2 ( XM_010849997.1 ) cDNA ORF clone, Bison bison bison -> XP_010848299.1 Bison bison bison mindbomb E3 ubiquitin protein ligase 2 (MIB2), mRNA.

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CloneID	OBi72259
Clone ID Related Accession (Same CDS sequence)	XM_010849997.1
Accession Version	XM_010849997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	E3 ubiquitin-protein ligase MIB2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494861.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCTACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG 
CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT 
GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT 
GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGGGGA CCGATGGCAG GGCCCCGGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC CGAGGAAGAT 
GCCAACCTGG ATGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAACACCCAG GGAAGCTGGT GGTGGAGGTG 
GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC ATTGACCTGC CGGATGATGA GGGCAACACG 
GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG 
AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT 
GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC 
TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGATGCC GGCTGCAGCG TCAACGCCGA GGATGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT 
GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC 
GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC 
AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCGC GCCGACGGGG CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG 
GAGCTGGCGN NNNNNNNNNC GCTGTCCACG GCCCCGCCCC CTGTCGTGGC TCACATCTCT 
GCTCGGGTTC TCTCGCAAGA CCTCAGCCCC GAGCCCCCGC CCCTGACCTG GACCTCTTCC 
GCAGACGGCA CGGAGGTGGT CAGCGCAGCC CCCGCGCCCG GCCCACCGCG GCAGTTGGTG 
GAGGAGCTGC AGAGCCGCTA CAGGCAGATG GAGGAGCGCA TCACCTGTCC GATTTGCATC 
GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG CGTGCGCGCC CTGTGGCGCG 
GCGCTCAGCG CCTGCCCGAT CTGCCGCCAG CCCATCCGCG ACCGCATCCA GATCTTCGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010848299.1
CDS	1189..4071
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRYGSPSTPDRTVVVQWDHGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRICFDYDLCTQCYMHNKHDLAHAFERYETAHSRPVMLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYRDHLPRLGKPAELQRRVSADGQPFQRGDKVKCLLDADVLREMQEGHGGWNPRMAKFIGQTGTVHRITDRGDVRVQFSHKTRWTFHPGALTKQNSCLVGEVVRVIDDLDTVKRLQAGHGEGTDGRAPALGRIGRVLKVFGDGNVGVLVSGKLWTFSPSCLVAYRPEEDANLDVAGRARENKSSLSVVLDKLRAQKSDLEHPGKLVVEVALGNVARALDLLRRHPEQVDTKNQGRTALQMAAYLGQVELVRLLLQAQAGIDLPDDEGNTALHYAALGNQPEAARVLLSSGCGANALNSTRSSALHVAVQRGFLEVVKVLCERGCDVNLPDAHADTPLHCAISAGTGASGIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHTLAVRRILARARQLVDAKKEDGFTALHLAAFNNHGEVAQVLIQEGHCDVNARNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLAADGSAGDPGPLQLLSRLQASGLPSSAELTAGAAMAYYLALEGADLSYANHRGRSPLDLAAEGRLLKALQGCAQRFRERQAGGSGSGGSAQGPRLVLSAPNTVTNLHVAAPTGPEAAECLVCSELAnullnullnullnullLSTAPPPVVAHISARVLSQDLSPEPPPLTWTSSADGTEVVSAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_010849997.1
Organism	Bison bison bison
Definition	Bison bison bison mindbomb E3 ubiquitin protein ligase 2 (MIB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010849997.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAAGC GGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC 
CGCTACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT 
CGCACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC 
CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG 
CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG 
GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG 
GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG 
CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT 
GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT 
CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT 
GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC 
GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT 
GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAGGGGA CCGATGGCAG GGCCCCGGCT 
CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC 
AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC CGAGGAAGAT 
GCCAACCTGG ATGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG 
GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAACACCCAG GGAAGCTGGT GGTGGAGGTG 
GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC 
ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG 
GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC ATTGACCTGC CGGATGATGA GGGCAACACG 
GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA 
GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG 
AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT 
GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC 
ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC 
TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG 
GCGCGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG 
GCCGCCTTCA ACAACCACGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT 
GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCGCTGCACC TGGCTGTGCA GCAGGCCCAC 
GTGGGGCTGG TGCCACTGCT GGTGGATGCC GGCTGCAGCG TCAACGCCGA GGATGAGGAG 
GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT 
GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT 
CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC 
GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT 
CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC 
AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTG 
ACCAACCTGC ATGTGGCCGC GCCGACGGGG CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG 
GAGCTGGCGN NNNNNNNNNC GCTGTCCACG GCCCCGCCCC CTGTCGTGGC TCACATCTCT 
GCTCGGGTTC TCTCGCAAGA CCTCAGCCCC GAGCCCCCGC CCCTGACCTG GACCTCTTCC 
GCAGACGGCA CGGAGGTGGT CAGCGCAGCC CCCGCGCCCG GCCCACCGCG GCAGTTGGTG 
GAGGAGCTGC AGAGCCGCTA CAGGCAGATG GAGGAGCGCA TCACCTGTCC GATTTGCATC 
GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG CGTGCGCGCC CTGTGGCGCG 
GCGCTCAGCG CCTGCCCGAT CTGCCGCCAG CCCATCCGCG ACCGCATCCA GATCTTCGTG 
TAG