CCDC146 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	CCDC146
Entrez Gene ID	104994494
Full Name	coiled-coil domain containing 146
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010848090.1	XP_010846392.1	coiled-coil domain-containing protein 146 isoform X2
XM_010848088.1	XP_010846390.1	coiled-coil domain-containing protein 146 isoform X1

Pathways from BioSystems
Gene Ontology

The following CCDC146 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCDC146 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi70821	XM_010848088.1 Latest version!	Bison bison bison coiled-coil domain containing 146 (CCDC146), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBi70822	XM_010848090.1 Latest version!	Bison bison bison coiled-coil domain containing 146 (CCDC146), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCDC146 ( XM_010848088.1 ) cDNA ORF clone, Bison bison bison -> XP_010846390.1 Bison bison bison coiled-coil domain containing 146 (CCDC146), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBi70821
Clone ID Related Accession (Same CDS sequence)	XM_010848088.1
Accession Version	XM_010848088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	coiled-coil domain-containing protein 146 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494832.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA GTACCACAGA CACAGACCAA GAAGAGGAAG AGGAGAAAGG TGGAAAGGCG 
CAAGAGGACC CCATTTATGC CACTGCACCC ACAATTAACA TCCAAGATGA GCGATTTGTT 
GATTTATCTA CAACTCCAGC TTTCATTTGT CTGCATGAGT TACATGCTCT GGGGAAACTT 
CCTGGAACCA GGATGGCAGA GTTAAAAGCC AAGTACACCT TGCTGCATGA TACCGTGATG 
AGCACACAAG AATCAGAGGT CCAGCTGCTA CAGGATGCCA AACGTTTCAC TGAGCAAATA 
CAAGAGCAGC AGTTTCACCT GCAGCAAGCT GATAACTTTC CACAGGCGTT CACCACCGAA 
GTTTCCAAAA TGAGAGAACA ACTTCTCAAG TATCAAAATG AATACAATGC AGTGAAGGAA 
AGAGAGTTCC ACAATCAGTA CAGACTGAAT AGCTTAGTGG AAGAAAAAAA CCTCATACTA 
AAGGAATTTG AGAAGATACC TAAGCCAGGA GAAATGGAGA AGAAGATGAG ACTATTGAGA 
GAAAGCACTG AAGAATTACG TAAGGAAGTA ATGCAGAAGA AATTAGAAAT TAAAAATTTA 
CGGGAAGATT TGACATCTAA GCAAAAGCAA TTACTAAAGG AGCAGAAGGA GCTAGAAGAA 
CTGCTTGAGT ATCAGGTCAC CTTAAAGGAT GAAGTGGTCC ACCATCAATC CATTCCTGTA 
CAAATTGGAA AAGAGATAGA AAAAACAACA CGCAAAAAAG TAGAAATGGA AAAGAAAAAA 
CTTGTCTTGG AATATGAACT CAAAGAGCTA AATGATTCCC TAAAGAAAGT CGAAACCAAA 
ATTAATGCTG TAATGGAAGA GAAGGAGGAT GTAATAAAGG AAGTTGAAGG CAAACGGGCC 
TTACTTGAAA TTAAAGAGCG AGAATATAAC CAATTGGTCA AGCTATTAGA ATTAACTAGA 
GAGAATGAAG CAAGTTCACT AACTGAAAGA GGGATCTTGG ATCTCAATTT ACGAAACTGT 
CTTATTGACA AGCATAACTA CCACGATGAA CTTTCTCGTA AGCAGAGAGA GAAAGAACGA 
GATTTTCGAA ATTTAAAAAA AATGGAGCTA CTCTTGAAAG TGTCCTGGGA TGCACTCAAT 
CAAACTCAAG CACTGCATCA GAGGCTTCTG TTAGAGATTG AAGCTATTCC TAAAGATGAT 
TCTACATTAT CTGAGAGAAG GCGAGAACTC CATAAGGAAG TTGAAGTAGC TAAGAGGAAT 
TTAACCCAGC AGAAAATCCT ATCAGAAGCT GAGTCTAAGT TAGTAGAACA GCAACTTGCT 
GAGGAAAACA AGCTTTTAAA GGAGCAAGAA AACATTCAAG AACTGCTTTT CAACCTTGTC 
CGTATGACTC AAATCAAAAT TGATGAGAAG GAGCAAAAAT CCAAGGATTT CCTGAAAGCT 
CAGCAAAAAT ATACCAGCAT TGTTAGGGAG ATCAAAGCAA AGGATCTTGA AATCCGGATA 
CACAGGAAGA AAAAACGTGA AATTCATCGG AGACTCAGAG AGTTTGCTAA ACTGTATGAC 
ACTATTCGAA ATGAAAGAAA CAAATTTATT AACTTACTTC ACAAAGCTCA CCAGAAAGTC 
AATGAAATAA AAGAAAGGCA TAAAATGTCA TTAAATGAAC TGGAGATTTT AAGAAATAGT 
GCAGTTACTC AAGAAAGAAA GCTACAAAAT TCAATGCTGA AGCATGCCAA CAACATTACC 
ATCAGAGAAA GCATGCAAAA CGATGTGTGC AAAATTGTAG CAAAACTTCA AGAAATGAAA 
GAGAAGAAAG AAGCCCAGTT AAATAACATC GACAGACTTG CCAATATGAT CACAATGATT 
GAAGAGGAGA TGGTTCAACT TCGGAAGAAA TATGAAAGAG CTGTTCAGCG TCGAAATGAA 
AGTGGTGTTC AGCTGATAGA GCGGGAAGAG GAAGTGTGCA TTTTTTATGA AAAAATAAAT 
ATCCAAGAGA AGATGAAGCT AAATGGAGAA ATTGAACTAC ATGTCCTGGA GGAAAAGATC 
CGATTCCTGA AACTCAAGAT TGCTGAGAAG CAAAGACAGA TTCACGTGAC CCGGAAATTA 
CTGCCAGTTA AGAGTGCTTT GGATGCTGAC TTAGCAGTGC TCCAGATTCA GTTTTCACAG 
TGTACAGACA AAATAAAAGA CCTGGAGAAA AAGTTTATAA ACCCTGAGGG TGAGAACAGA 
ACACGCTTAC TTCCAGGGAA AGATATGACT GAGGAAGAAA TGATCAAAAA AATGGATGAG 
CTGGAACTAC AGCTGGCCAA GAAGGAGGAG AAGTTGCTGG AGAAGGATTT CATCTATGAA 
CAGGTCTCCC GGCTCACAGA CAGGCTCTAC AGCAAGACGC AGGCCTGCAA ACAGGACACG 
CTACTCTTAG CCAAGAAGAT GAATGGCTAC CAGAAAAAGA TAAAAGATGC TACTGAAAAA 
ATGATGGCTC TTGTTGCTGA GCTGTCCATG AAACAAGCTA TGGCCATTGA ACTCCAAAAG 
GAAGTCAAGG AGAAGGAAGA GTTCATCTTC ACTTGCAATT CCAGGATGGA AAAGGGTCTT 
CCCCTCAATA AAGAGATTGA GAGAGAATGG TTGAAAGTAC TTCGAGATGA AGAAATGTAT 
GCCATGGCCA TTGCTGAAAA GTCTCGGGAG TTCTTGGAAG CTGAAACTCG CCAGCTGCCC 
AATGGTGTTT ACACAACTGC GGAGCAGCGT CCAAATGCCT ACATCCCAGA GGCAGAGGCC 
ACTCTTCCTC TGCCAAAACC GTATGGCGCT CTGGCTCCTT TCAAACCCAG TGAACCTGGG 
GCTAACATAA GACACATAAG GAAACCTGTT ATAAAGCCAA TTGAAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010846390.1
CDS	187..3057
Translation	MEDSTTDTDQEEEEEKGGKAQEDPIYATAPTINIQDERFVDLSTTPAFICLHELHALGKLPGTRMAELKAKYTLLHDTVMSTQESEVQLLQDAKRFTEQIQEQQFHLQQADNFPQAFTTEVSKMREQLLKYQNEYNAVKEREFHNQYRLNSLVEEKNLILKEFEKIPKPGEMEKKMRLLRESTEELRKEVMQKKLEIKNLREDLTSKQKQLLKEQKELEELLEYQVTLKDEVVHHQSIPVQIGKEIEKTTRKKVEMEKKKLVLEYELKELNDSLKKVETKINAVMEEKEDVIKEVEGKRALLEIKEREYNQLVKLLELTRENEASSLTERGILDLNLRNCLIDKHNYHDELSRKQREKERDFRNLKKMELLLKVSWDALNQTQALHQRLLLEIEAIPKDDSTLSERRRELHKEVEVAKRNLTQQKILSEAESKLVEQQLAEENKLLKEQENIQELLFNLVRMTQIKIDEKEQKSKDFLKAQQKYTSIVREIKAKDLEIRIHRKKKREIHRRLREFAKLYDTIRNERNKFINLLHKAHQKVNEIKERHKMSLNELEILRNSAVTQERKLQNSMLKHANNITIRESMQNDVCKIVAKLQEMKEKKEAQLNNIDRLANMITMIEEEMVQLRKKYERAVQRRNESGVQLIEREEEVCIFYEKINIQEKMKLNGEIELHVLEEKIRFLKLKIAEKQRQIHVTRKLLPVKSALDADLAVLQIQFSQCTDKIKDLEKKFINPEGENRTRLLPGKDMTEEEMIKKMDELELQLAKKEEKLLEKDFIYEQVSRLTDRLYSKTQACKQDTLLLAKKMNGYQKKIKDATEKMMALVAELSMKQAMAIELQKEVKEKEEFIFTCNSRMEKGLPLNKEIEREWLKVLRDEEMYAMAIAEKSREFLEAETRQLPNGVYTTAEQRPNAYIPEAEATLPLPKPYGALAPFKPSEPGANIRHIRKPVIKPIEI

Target ORF information:

RefSeq Version	XM_010848088.1
Organism	Bison bison bison
Definition	Bison bison bison coiled-coil domain containing 146 (CCDC146), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010848088.1

ORF Insert Sequence:

ATGGAAGACA GTACCACAGA CACAGACCAA GAAGAGGAAG AGGAGAAAGG TGGAAAGGCG 
CAAGAGGACC CCATTTATGC CACTGCACCC ACAATTAACA TCCAAGATGA GCGATTTGTT 
GATTTATCTA CAACTCCAGC TTTCATTTGT CTGCATGAGT TACATGCTCT GGGGAAACTT 
CCTGGAACCA GGATGGCAGA GTTAAAAGCC AAGTACACCT TGCTGCATGA TACCGTGATG 
AGCACACAAG AATCAGAGGT CCAGCTGCTA CAGGATGCCA AACGTTTCAC TGAGCAAATA 
CAAGAGCAGC AGTTTCACCT GCAGCAAGCT GATAACTTTC CACAGGCGTT CACCACCGAA 
GTTTCCAAAA TGAGAGAACA ACTTCTCAAG TATCAAAATG AATACAATGC AGTGAAGGAA 
AGAGAGTTCC ACAATCAGTA CAGACTGAAT AGCTTAGTGG AAGAAAAAAA CCTCATACTA 
AAGGAATTTG AGAAGATACC TAAGCCAGGA GAAATGGAGA AGAAGATGAG ACTATTGAGA 
GAAAGCACTG AAGAATTACG TAAGGAAGTA ATGCAGAAGA AATTAGAAAT TAAAAATTTA 
CGGGAAGATT TGACATCTAA GCAAAAGCAA TTACTAAAGG AGCAGAAGGA GCTAGAAGAA 
CTGCTTGAGT ATCAGGTCAC CTTAAAGGAT GAAGTGGTCC ACCATCAATC CATTCCTGTA 
CAAATTGGAA AAGAGATAGA AAAAACAACA CGCAAAAAAG TAGAAATGGA AAAGAAAAAA 
CTTGTCTTGG AATATGAACT CAAAGAGCTA AATGATTCCC TAAAGAAAGT CGAAACCAAA 
ATTAATGCTG TAATGGAAGA GAAGGAGGAT GTAATAAAGG AAGTTGAAGG CAAACGGGCC 
TTACTTGAAA TTAAAGAGCG AGAATATAAC CAATTGGTCA AGCTATTAGA ATTAACTAGA 
GAGAATGAAG CAAGTTCACT AACTGAAAGA GGGATCTTGG ATCTCAATTT ACGAAACTGT 
CTTATTGACA AGCATAACTA CCACGATGAA CTTTCTCGTA AGCAGAGAGA GAAAGAACGA 
GATTTTCGAA ATTTAAAAAA AATGGAGCTA CTCTTGAAAG TGTCCTGGGA TGCACTCAAT 
CAAACTCAAG CACTGCATCA GAGGCTTCTG TTAGAGATTG AAGCTATTCC TAAAGATGAT 
TCTACATTAT CTGAGAGAAG GCGAGAACTC CATAAGGAAG TTGAAGTAGC TAAGAGGAAT 
TTAACCCAGC AGAAAATCCT ATCAGAAGCT GAGTCTAAGT TAGTAGAACA GCAACTTGCT 
GAGGAAAACA AGCTTTTAAA GGAGCAAGAA AACATTCAAG AACTGCTTTT CAACCTTGTC 
CGTATGACTC AAATCAAAAT TGATGAGAAG GAGCAAAAAT CCAAGGATTT CCTGAAAGCT 
CAGCAAAAAT ATACCAGCAT TGTTAGGGAG ATCAAAGCAA AGGATCTTGA AATCCGGATA 
CACAGGAAGA AAAAACGTGA AATTCATCGG AGACTCAGAG AGTTTGCTAA ACTGTATGAC 
ACTATTCGAA ATGAAAGAAA CAAATTTATT AACTTACTTC ACAAAGCTCA CCAGAAAGTC 
AATGAAATAA AAGAAAGGCA TAAAATGTCA TTAAATGAAC TGGAGATTTT AAGAAATAGT 
GCAGTTACTC AAGAAAGAAA GCTACAAAAT TCAATGCTGA AGCATGCCAA CAACATTACC 
ATCAGAGAAA GCATGCAAAA CGATGTGTGC AAAATTGTAG CAAAACTTCA AGAAATGAAA 
GAGAAGAAAG AAGCCCAGTT AAATAACATC GACAGACTTG CCAATATGAT CACAATGATT 
GAAGAGGAGA TGGTTCAACT TCGGAAGAAA TATGAAAGAG CTGTTCAGCG TCGAAATGAA 
AGTGGTGTTC AGCTGATAGA GCGGGAAGAG GAAGTGTGCA TTTTTTATGA AAAAATAAAT 
ATCCAAGAGA AGATGAAGCT AAATGGAGAA ATTGAACTAC ATGTCCTGGA GGAAAAGATC 
CGATTCCTGA AACTCAAGAT TGCTGAGAAG CAAAGACAGA TTCACGTGAC CCGGAAATTA 
CTGCCAGTTA AGAGTGCTTT GGATGCTGAC TTAGCAGTGC TCCAGATTCA GTTTTCACAG 
TGTACAGACA AAATAAAAGA CCTGGAGAAA AAGTTTATAA ACCCTGAGGG TGAGAACAGA 
ACACGCTTAC TTCCAGGGAA AGATATGACT GAGGAAGAAA TGATCAAAAA AATGGATGAG 
CTGGAACTAC AGCTGGCCAA GAAGGAGGAG AAGTTGCTGG AGAAGGATTT CATCTATGAA 
CAGGTCTCCC GGCTCACAGA CAGGCTCTAC AGCAAGACGC AGGCCTGCAA ACAGGACACG 
CTACTCTTAG CCAAGAAGAT GAATGGCTAC CAGAAAAAGA TAAAAGATGC TACTGAAAAA 
ATGATGGCTC TTGTTGCTGA GCTGTCCATG AAACAAGCTA TGGCCATTGA ACTCCAAAAG 
GAAGTCAAGG AGAAGGAAGA GTTCATCTTC ACTTGCAATT CCAGGATGGA AAAGGGTCTT 
CCCCTCAATA AAGAGATTGA GAGAGAATGG TTGAAAGTAC TTCGAGATGA AGAAATGTAT 
GCCATGGCCA TTGCTGAAAA GTCTCGGGAG TTCTTGGAAG CTGAAACTCG CCAGCTGCCC 
AATGGTGTTT ACACAACTGC GGAGCAGCGT CCAAATGCCT ACATCCCAGA GGCAGAGGCC 
ACTCTTCCTC TGCCAAAACC GTATGGCGCT CTGGCTCCTT TCAAACCCAG TGAACCTGGG 
GCTAACATAA GACACATAAG GAAACCTGTT ATAAAGCCAA TTGAAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCDC146 ( XM_010848090.1 ) cDNA ORF clone, Bison bison bison -> XP_010846392.1 Bison bison bison coiled-coil domain containing 146 (CCDC146), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBi70822
Clone ID Related Accession (Same CDS sequence)	XM_010848090.1
Accession Version	XM_010848090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	coiled-coil domain-containing protein 146 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494832.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA GTACCACAGA CACAGACCAA GAAGAGGAAG AGGAGAAAGG TGGAAAGGCG 
CAAGAGGACC CCATTTATGC CACTGCACCC ACAATTAACA TCCAAGATGA GCGATTTGTT 
GATTTATCTA CAACTCCAGC TTTCATTTGT CTGCATGAGT TACATGCTCT GGGGAAACTT 
CCTGGAACCA GGATGGCAGA GTTAAAAGCC AAGTACACCT TGCTGCATGA TACCGTGATG 
AGCACACAAG AATCAGAGGT CCAGCTGCTA CAGGATGCCA AACGTTTCAC TGAGCAAATA 
CAAGAGCAGC AGTTTCACCT GCAGCAAGCT GATAACTTTC CACAGGCGTT CACCACCGAA 
GTTTCCAAAA TGAGAGAACA ACTTCTCAAG TATCAAAATG AATACAATGC AGTGAAGGAA 
AGAGAGTTCC ACAATCAGTA CAGACTGAAT AGCTTAGTGG AAGAAAAAAA CCTCATACTA 
AAGGAATTTG AGAAGATACC TAAGCCAGGA GAAATGGAGA AGAAGATGAG ACTATTGAGA 
GAAAGCACTG AAGAATTACG TAAGGAAGTA ATGCAGAAGA AATTAGAAAT TAAAAATTTA 
CGGGAAGATT TGACATCTAA GCAAAAGCAA TTACTAAAGG AGCAGAAGGA GCTAGAAGAA 
CTGCTTGAGT ATCAGGTCAC CTTAAAGGAT GAAGTGGTCC ACCATCAATC CATTCCTGTA 
CAAATTGGAA AAGAGATAGA AAAAACAACA CGCAAAAAAG TAGAAATGGA AAAGAAAAAA 
CTTGTCTTGG AATATGAACT CAAAGAGCTA AATGATTCCC TAAAGAAAGT CGAAACCAAA 
ATTAATGCTG TAATGGAAGA GAAGGAGGAT GTAATAAAGG AAGTTGAAGG CAAACGGGCC 
TTACTTGAAA TTAAAGAGCG AGAATATAAC CAATTGGTCA AGCTATTAGA ATTAACTAGA 
GAGAATGAAG CAAGTTCACT AACTGAAAGA GGGATCTTGG ATCTCAATTT ACGAAACTGT 
CTTATTGACA AGCATAACTA CCACGATGAA CTTTCTCGTA AGCAGAGAGA GAAAGAACGA 
GATTTTCGAA ATTTAAAAAA AATGGAGCTA CTCTTGAAAG TGTCCTGGGA TGCACTCAAT 
CAAACTCAAG CACTGCATCA GAGGCTTCTG TTAGAGATTG AAGCTATTCC TAAAGATGAT 
TCTACATTAT CTGAGAGAAG GCGAGAACTC CATAAGGAAG TTGAAGTAGC TAAGAGGAAT 
TTAACCCAGC AGCAAAAATA TACCAGCATT GTTAGGGAGA TCAAAGCAAA GGATCTTGAA 
ATCCGGATAC ACAGGAAGAA AAAACGTGAA ATTCATCGGA GACTCAGAGA GTTTGCTAAA 
CTGTATGACA CTATTCGAAA TGAAAGAAAC AAATTTATTA ACTTACTTCA CAAAGCTCAC 
CAGAAAGTCA ATGAAATAAA AGAAAGGCAT AAAATGTCAT TAAATGAACT GGAGATTTTA 
AGAAATAGTG CAGTTACTCA AGAAAGAAAG CTACAAAATT CAATGCTGAA GCATGCCAAC 
AACATTACCA TCAGAGAAAG CATGCAAAAC GATGTGTGCA AAATTGTAGC AAAACTTCAA 
GAAATGAAAG AGAAGAAAGA AGCCCAGTTA AATAACATCG ACAGACTTGC CAATATGATC 
ACAATGATTG AAGAGGAGAT GGTTCAACTT CGGAAGAAAT ATGAAAGAGC TGTTCAGCGT 
CGAAATGAAA GTGGTGTTCA GCTGATAGAG CGGGAAGAGG AAGTGTGCAT TTTTTATGAA 
AAAATAAATA TCCAAGAGAA GATGAAGCTA AATGGAGAAA TTGAACTACA TGTCCTGGAG 
GAAAAGATCC GATTCCTGAA ACTCAAGATT GCTGAGAAGC AAAGACAGAT TCACGTGACC 
CGGAAATTAC TGCCAGTTAA GAGTGCTTTG GATGCTGACT TAGCAGTGCT CCAGATTCAG 
TTTTCACAGT GTACAGACAA AATAAAAGAC CTGGAGAAAA AGTTTATAAA CCCTGAGGGT 
GAGAACAGAA CACGCTTACT TCCAGGGAAA GATATGACTG AGGAAGAAAT GATCAAAAAA 
ATGGATGAGC TGGAACTACA GCTGGCCAAG AAGGAGGAGA AGTTGCTGGA GAAGGATTTC 
ATCTATGAAC AGGTCTCCCG GCTCACAGAC AGGCTCTACA GCAAGACGCA GGCCTGCAAA 
CAGGACACGC TACTCTTAGC CAAGAAGATG AATGGCTACC AGAAAAAGAT AAAAGATGCT 
ACTGAAAAAA TGATGGCTCT TGTTGCTGAG CTGTCCATGA AACAAGCTAT GGCCATTGAA 
CTCCAAAAGG AAGTCAAGGA GAAGGAAGAG TTCATCTTCA CTTGCAATTC CAGGATGGAA 
AAGGGTCTTC CCCTCAATAA AGAGATTGAG AGAGAATGGT TGAAAGTACT TCGAGATGAA 
GAAATGTATG CCATGGCCAT TGCTGAAAAG TCTCGGGAGT TCTTGGAAGC TGAAACTCGC 
CAGCTGCCCA ATGGTGTTTA CACAACTGCG GAGCAGCGTC CAAATGCCTA CATCCCAGAG 
GCAGAGGCCA CTCTTCCTCT GCCAAAACCG TATGGCGCTC TGGCTCCTTT CAAACCCAGT 
GAACCTGGGG CTAACATAAG ACACATAAGG AAACCTGTTA TAAAGCCAAT TGAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010846392.1
CDS	187..2886
Translation	MEDSTTDTDQEEEEEKGGKAQEDPIYATAPTINIQDERFVDLSTTPAFICLHELHALGKLPGTRMAELKAKYTLLHDTVMSTQESEVQLLQDAKRFTEQIQEQQFHLQQADNFPQAFTTEVSKMREQLLKYQNEYNAVKEREFHNQYRLNSLVEEKNLILKEFEKIPKPGEMEKKMRLLRESTEELRKEVMQKKLEIKNLREDLTSKQKQLLKEQKELEELLEYQVTLKDEVVHHQSIPVQIGKEIEKTTRKKVEMEKKKLVLEYELKELNDSLKKVETKINAVMEEKEDVIKEVEGKRALLEIKEREYNQLVKLLELTRENEASSLTERGILDLNLRNCLIDKHNYHDELSRKQREKERDFRNLKKMELLLKVSWDALNQTQALHQRLLLEIEAIPKDDSTLSERRRELHKEVEVAKRNLTQQQKYTSIVREIKAKDLEIRIHRKKKREIHRRLREFAKLYDTIRNERNKFINLLHKAHQKVNEIKERHKMSLNELEILRNSAVTQERKLQNSMLKHANNITIRESMQNDVCKIVAKLQEMKEKKEAQLNNIDRLANMITMIEEEMVQLRKKYERAVQRRNESGVQLIEREEEVCIFYEKINIQEKMKLNGEIELHVLEEKIRFLKLKIAEKQRQIHVTRKLLPVKSALDADLAVLQIQFSQCTDKIKDLEKKFINPEGENRTRLLPGKDMTEEEMIKKMDELELQLAKKEEKLLEKDFIYEQVSRLTDRLYSKTQACKQDTLLLAKKMNGYQKKIKDATEKMMALVAELSMKQAMAIELQKEVKEKEEFIFTCNSRMEKGLPLNKEIEREWLKVLRDEEMYAMAIAEKSREFLEAETRQLPNGVYTTAEQRPNAYIPEAEATLPLPKPYGALAPFKPSEPGANIRHIRKPVIKPIEI

Target ORF information:

RefSeq Version	XM_010848090.1
Organism	Bison bison bison
Definition	Bison bison bison coiled-coil domain containing 146 (CCDC146), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010848090.1

ORF Insert Sequence:

ATGGAAGACA GTACCACAGA CACAGACCAA GAAGAGGAAG AGGAGAAAGG TGGAAAGGCG 
CAAGAGGACC CCATTTATGC CACTGCACCC ACAATTAACA TCCAAGATGA GCGATTTGTT 
GATTTATCTA CAACTCCAGC TTTCATTTGT CTGCATGAGT TACATGCTCT GGGGAAACTT 
CCTGGAACCA GGATGGCAGA GTTAAAAGCC AAGTACACCT TGCTGCATGA TACCGTGATG 
AGCACACAAG AATCAGAGGT CCAGCTGCTA CAGGATGCCA AACGTTTCAC TGAGCAAATA 
CAAGAGCAGC AGTTTCACCT GCAGCAAGCT GATAACTTTC CACAGGCGTT CACCACCGAA 
GTTTCCAAAA TGAGAGAACA ACTTCTCAAG TATCAAAATG AATACAATGC AGTGAAGGAA 
AGAGAGTTCC ACAATCAGTA CAGACTGAAT AGCTTAGTGG AAGAAAAAAA CCTCATACTA 
AAGGAATTTG AGAAGATACC TAAGCCAGGA GAAATGGAGA AGAAGATGAG ACTATTGAGA 
GAAAGCACTG AAGAATTACG TAAGGAAGTA ATGCAGAAGA AATTAGAAAT TAAAAATTTA 
CGGGAAGATT TGACATCTAA GCAAAAGCAA TTACTAAAGG AGCAGAAGGA GCTAGAAGAA 
CTGCTTGAGT ATCAGGTCAC CTTAAAGGAT GAAGTGGTCC ACCATCAATC CATTCCTGTA 
CAAATTGGAA AAGAGATAGA AAAAACAACA CGCAAAAAAG TAGAAATGGA AAAGAAAAAA 
CTTGTCTTGG AATATGAACT CAAAGAGCTA AATGATTCCC TAAAGAAAGT CGAAACCAAA 
ATTAATGCTG TAATGGAAGA GAAGGAGGAT GTAATAAAGG AAGTTGAAGG CAAACGGGCC 
TTACTTGAAA TTAAAGAGCG AGAATATAAC CAATTGGTCA AGCTATTAGA ATTAACTAGA 
GAGAATGAAG CAAGTTCACT AACTGAAAGA GGGATCTTGG ATCTCAATTT ACGAAACTGT 
CTTATTGACA AGCATAACTA CCACGATGAA CTTTCTCGTA AGCAGAGAGA GAAAGAACGA 
GATTTTCGAA ATTTAAAAAA AATGGAGCTA CTCTTGAAAG TGTCCTGGGA TGCACTCAAT 
CAAACTCAAG CACTGCATCA GAGGCTTCTG TTAGAGATTG AAGCTATTCC TAAAGATGAT 
TCTACATTAT CTGAGAGAAG GCGAGAACTC CATAAGGAAG TTGAAGTAGC TAAGAGGAAT 
TTAACCCAGC AGCAAAAATA TACCAGCATT GTTAGGGAGA TCAAAGCAAA GGATCTTGAA 
ATCCGGATAC ACAGGAAGAA AAAACGTGAA ATTCATCGGA GACTCAGAGA GTTTGCTAAA 
CTGTATGACA CTATTCGAAA TGAAAGAAAC AAATTTATTA ACTTACTTCA CAAAGCTCAC 
CAGAAAGTCA ATGAAATAAA AGAAAGGCAT AAAATGTCAT TAAATGAACT GGAGATTTTA 
AGAAATAGTG CAGTTACTCA AGAAAGAAAG CTACAAAATT CAATGCTGAA GCATGCCAAC 
AACATTACCA TCAGAGAAAG CATGCAAAAC GATGTGTGCA AAATTGTAGC AAAACTTCAA 
GAAATGAAAG AGAAGAAAGA AGCCCAGTTA AATAACATCG ACAGACTTGC CAATATGATC 
ACAATGATTG AAGAGGAGAT GGTTCAACTT CGGAAGAAAT ATGAAAGAGC TGTTCAGCGT 
CGAAATGAAA GTGGTGTTCA GCTGATAGAG CGGGAAGAGG AAGTGTGCAT TTTTTATGAA 
AAAATAAATA TCCAAGAGAA GATGAAGCTA AATGGAGAAA TTGAACTACA TGTCCTGGAG 
GAAAAGATCC GATTCCTGAA ACTCAAGATT GCTGAGAAGC AAAGACAGAT TCACGTGACC 
CGGAAATTAC TGCCAGTTAA GAGTGCTTTG GATGCTGACT TAGCAGTGCT CCAGATTCAG 
TTTTCACAGT GTACAGACAA AATAAAAGAC CTGGAGAAAA AGTTTATAAA CCCTGAGGGT 
GAGAACAGAA CACGCTTACT TCCAGGGAAA GATATGACTG AGGAAGAAAT GATCAAAAAA 
ATGGATGAGC TGGAACTACA GCTGGCCAAG AAGGAGGAGA AGTTGCTGGA GAAGGATTTC 
ATCTATGAAC AGGTCTCCCG GCTCACAGAC AGGCTCTACA GCAAGACGCA GGCCTGCAAA 
CAGGACACGC TACTCTTAGC CAAGAAGATG AATGGCTACC AGAAAAAGAT AAAAGATGCT 
ACTGAAAAAA TGATGGCTCT TGTTGCTGAG CTGTCCATGA AACAAGCTAT GGCCATTGAA 
CTCCAAAAGG AAGTCAAGGA GAAGGAAGAG TTCATCTTCA CTTGCAATTC CAGGATGGAA 
AAGGGTCTTC CCCTCAATAA AGAGATTGAG AGAGAATGGT TGAAAGTACT TCGAGATGAA 
GAAATGTATG CCATGGCCAT TGCTGAAAAG TCTCGGGAGT TCTTGGAAGC TGAAACTCGC 
CAGCTGCCCA ATGGTGTTTA CACAACTGCG GAGCAGCGTC CAAATGCCTA CATCCCAGAG 
GCAGAGGCCA CTCTTCCTCT GCCAAAACCG TATGGCGCTC TGGCTCCTTT CAAACCCAGT 
GAACCTGGGG CTAACATAAG ACACATAAGG AAACCTGTTA TAAAGCCAAT TGAAATCTGA