BGAL5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	BGAL5
Entrez Gene ID	841080
Full Name	beta-galactosidase 5
Synonyms	AtBGAL5,F27F5.20,F27F5_20,beta-galactosidase 5
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_103587.3	NP_175127.1	beta-galactosidase 5
NM_001333243.1	NP_001320473.1	beta-galactosidase 5

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate the transcript accumulation of the beta-galactosidases (BGAL) genes AtBGAL1 (At3g13750), AtBGAL2 (At3g52840), AtBGAL3 (At4g36360), AtBGAL4 (At5g56870), AtBGAL5 (At1g45130) and AtBGAL12 (At4g26140) along the plant development, as well as their subcellular location by the construction of transgenic plants producing the enhanced green fluorescent protein (eGFP) fused to the six BGAL proteins.
Title: Subcellular location of Arabidopsis thaliana subfamily a1 ?-galactosidases and developmental regulation of transcript levels?of?their coding genes.

It is shown by dot-immunoblotting that At1g45130 is the gene expressing Gal-5 which is associated with the cell wall in Arabidopsis.
Title: The Arabidopsis At1g45130 and At3g52840 genes encode beta-galactosidases with activity toward cell wall polysaccharides.

The following BGAL5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BGAL5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb25703	NM_103587.3 Latest version!	Arabidopsis thaliana beta-galactosidase 5 (BGAL5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb34213	NM_001333243.1 Latest version!	Arabidopsis thaliana beta-galactosidase 5 (BGAL5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BGAL5 ( NM_103587.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_175127.1 Arabidopsis thaliana beta-galactosidase 5 (BGAL5), mRNA.

Price & Availability↑

CloneID	OAb25703
Clone ID Related Accession (Same CDS sequence)	NM_103587.3
Accession Version	NM_103587.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	beta-galactosidase 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_103587.2.

ATGGGAACAA CGATCTTGGT TCTCTCCAAG ATCCTAACTT TCTTACTAAC AACAATGCTG 
ATTGGATCCA GTGTGATCCA GTGTAGTAGT GTAACCTACG ATAAGAAAGC TATCGTCATC 
AATGGCCACC GTAGAATCCT CCTCTCTGGT TCTATTCACT ACCCAAGAAG CACTCCTGAG 
ATGTGGGAAG ATCTTATAAA GAAAGCTAAA GACGGTGGCT TGGATGTTAT TGATACTTAT 
GTTTTCTGGA ATGGTCATGA ACCTTCTCCT GGAACTTACA ATTTCGAAGG AAGATATGAT 
TTGGTAAGAT TCATTAAGAC GATTCAGGAA GTGGGTCTTT ATGTTCATCT CAGAATTGGT 
CCTTATGTTT GTGCAGAATG GAATTTTGGA GGGTTTCCTG TGTGGTTGAA GTATGTAGAT 
GGGATTAGTT TCAGAACTGA CAATGGACCC TTCAAGTCTG CAATGCAAGG ATTCACAGAG 
AAGATTGTTC AGATGATGAA AGAACATAGA TTCTTTGCGT CACAAGGTGG ACCTATCATT 
CTTTCTCAGA TTGAGAATGA GTTTGAACCA GATCTTAAAG GGCTTGGACC GGCTGGTCAC 
TCGTATGTCA ATTGGGCTGC GAAAATGGCG GTCGGTTTAA ACACGGGAGT ACCGTGGGTG 
ATGTGCAAGG AAGATGATGC ACCTGACCCA ATTATAAACA CTTGCAACGG ATTCTACTGT 
GATTATTTTA CTCCAAATAA ACCGTATAAG CCAACTATGT GGACAGAGGC ATGGAGTGGC 
TGGTTCACAG AGTTTGGTGG AACTGTTCCT AAACGACCTG TAGAGGATCT AGCATTTGGA 
GTAGCTCGTT TCATTCAAAA GGGCGGTTCG TATATAAACT ACTACATGTA CCATGGAGGA 
ACAAACTTTG GACGCACCGC AGGAGGTCCA TTTATCACCA CTAGTTATGA CTATGATGCT 
CCTATCGATG AATACGGGTT GGTCCAAGAG CCCAAGTACA GTCATCTTAA GCAGCTTCAT 
CAGGCAATCA AGCAATGTGA AGCTGCCTTA GTTTCTTCTG ATCCACATGT TACTAAACTA 
GGAAACTACG AGGAGGCTCA TGTGTTCACT GCTGGCAAAG GAAGTTGTGT AGCTTTCTTA 
ACGAACTATC ACATGAATGC ACCTGCAAAA GTAGTGTTCA ATAACCGACA CTATACTCTA 
CCTGCTTGGT CCATCAGCAT TCTTCCAGAT TGTAGAAACG TTGTTTTCAA CACTGCGACG 
GTTGCTGCAA AGACATCACA TGTGCAAATG GTGCCATCTG GTTCCATATT GTACTCGGTT 
GCTAGATACG ATGAAGATAT TGCTACTTAT GGAAACCGTG GGACAATCAC AGCTCGTGGA 
TTGTTGGAGC AGGTTAATGT TACACGAGAT ACAACTGATT ACCTGTGGTA CACAACCAGT 
GTGGATATTA AGGCATCAGA ATCATTCTTG CGTGGAGGAA AATGGCCAAC TCTTACAGTG 
GATTCTGCAG GGCATGCTGT TCATGTGTTT GTCAATGGAC ATTTTTACGG ATCTGCCTTT 
GGAACAAGAG AAAACAGAAA GTTTTCATTT AGCTCCCAAG TCAATCTACG AGGTGGAGCT 
AACAAAATCG CACTACTGAG TGTAGCAGTT GGGTTGCCGA ATGTTGGACC ACATTTTGAG 
ACGTGGGCCA CAGGAATCGT CGGGTCTGTG GTGCTTCATG GCCTTGACGA GGGTAACAAA 
GACTTGAGTT GGCAGAAATG GACTTATCAG GCTGGTCTGC GAGGGGAATC AATGAACTTG 
GTCTCTCCTA CTGAGGACTC CTCTGTTGAT TGGATCAAAG GCTCATTGGC TAAGCAAAAC 
AAACAACCTT TGACATGGTA CAAGGCCTAT TTTGACGCGC CTAGAGGGAA CGAGCCGCTG 
GCTTTGGATC TAAAGAGTAT GGGGAAAGGG CAAGCTTGGA TAAACGGGCA AAGCATAGGG 
AGATACTGGA TGGCATTTGC CAAAGGAGAC TGCGGAAGTT GTAACTACGC TGGAACATAC 
AGGCAGAACA AATGCCAGTC TGGTTGTGGC GAGCCGACAC AAAGATGGTA TCATGTTCCG 
CGTTCGTGGT TGAAGCCAAA AGGGAACTTG TTAGTACTTT TTGAAGAACT TGGTGGAGAT 
ATATCCAAAG TCTCTGTTGT GAAGAGATCA GTAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_175127.1
CDS	337..2535
Translation	MGTTILVLSKILTFLLTTMLIGSSVIQCSSVTYDKKAIVINGHRRILLSGSIHYPRSTPEMWEDLIKKAKDGGLDVIDTYVFWNGHEPSPGTYNFEGRYDLVRFIKTIQEVGLYVHLRIGPYVCAEWNFGGFPVWLKYVDGISFRTDNGPFKSAMQGFTEKIVQMMKEHRFFASQGGPIILSQIENEFEPDLKGLGPAGHSYVNWAAKMAVGLNTGVPWVMCKEDDAPDPIINTCNGFYCDYFTPNKPYKPTMWTEAWSGWFTEFGGTVPKRPVEDLAFGVARFIQKGGSYINYYMYHGGTNFGRTAGGPFITTSYDYDAPIDEYGLVQEPKYSHLKQLHQAIKQCEAALVSSDPHVTKLGNYEEAHVFTAGKGSCVAFLTNYHMNAPAKVVFNNRHYTLPAWSISILPDCRNVVFNTATVAAKTSHVQMVPSGSILYSVARYDEDIATYGNRGTITARGLLEQVNVTRDTTDYLWYTTSVDIKASESFLRGGKWPTLTVDSAGHAVHVFVNGHFYGSAFGTRENRKFSFSSQVNLRGGANKIALLSVAVGLPNVGPHFETWATGIVGSVVLHGLDEGNKDLSWQKWTYQAGLRGESMNLVSPTEDSSVDWIKGSLAKQNKQPLTWYKAYFDAPRGNEPLALDLKSMGKGQAWINGQSIGRYWMAFAKGDCGSCNYAGTYRQNKCQSGCGEPTQRWYHVPRSWLKPKGNLLVLFEELGGDISKVSVVKRSVN

Target ORF information:

RefSeq Version	NM_103587.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana beta-galactosidase 5 (BGAL5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_103587.3

ORF Insert Sequence:

ATGGGAACAA CGATCTTGGT TCTCTCCAAG ATCCTAACTT TCTTACTAAC AACAATGCTG 
ATTGGATCCA GTGTGATCCA GTGTAGTAGT GTAACCTACG ATAAGAAAGC TATCGTCATC 
AATGGCCACC GTAGAATCCT CCTCTCTGGT TCTATTCACT ACCCAAGAAG CACTCCTGAG 
ATGTGGGAAG ATCTTATAAA GAAAGCTAAA GACGGTGGCT TGGATGTTAT TGATACTTAT 
GTTTTCTGGA ATGGTCATGA ACCTTCTCCT GGAACTTACA ATTTCGAAGG AAGATATGAT 
TTGGTAAGAT TCATTAAGAC GATTCAGGAA GTGGGTCTTT ATGTTCATCT CAGAATTGGT 
CCTTATGTTT GTGCAGAATG GAATTTTGGA GGGTTTCCTG TGTGGTTGAA GTATGTAGAT 
GGGATTAGTT TCAGAACTGA CAATGGACCC TTCAAGTCTG CAATGCAAGG ATTCACAGAG 
AAGATTGTTC AGATGATGAA AGAACATAGA TTCTTTGCGT CACAAGGTGG ACCTATCATT 
CTTTCTCAGA TTGAGAATGA GTTTGAACCA GATCTTAAAG GGCTTGGACC GGCTGGTCAC 
TCGTATGTCA ATTGGGCTGC GAAAATGGCG GTCGGTTTAA ACACGGGAGT ACCGTGGGTG 
ATGTGCAAGG AAGATGATGC ACCTGACCCA ATTATAAACA CTTGCAACGG ATTCTACTGT 
GATTATTTTA CTCCAAATAA ACCGTATAAG CCAACTATGT GGACAGAGGC ATGGAGTGGC 
TGGTTCACAG AGTTTGGTGG AACTGTTCCT AAACGACCTG TAGAGGATCT AGCATTTGGA 
GTAGCTCGTT TCATTCAAAA GGGCGGTTCG TATATAAACT ACTACATGTA CCATGGAGGA 
ACAAACTTTG GACGCACCGC AGGAGGTCCA TTTATCACCA CTAGTTATGA CTATGATGCT 
CCTATCGATG AATACGGGTT GGTCCAAGAG CCCAAGTACA GTCATCTTAA GCAGCTTCAT 
CAGGCAATCA AGCAATGTGA AGCTGCCTTA GTTTCTTCTG ATCCACATGT TACTAAACTA 
GGAAACTACG AGGAGGCTCA TGTGTTCACT GCTGGCAAAG GAAGTTGTGT AGCTTTCTTA 
ACGAACTATC ACATGAATGC ACCTGCAAAA GTAGTGTTCA ATAACCGACA CTATACTCTA 
CCTGCTTGGT CCATCAGCAT TCTTCCAGAT TGTAGAAACG TTGTTTTCAA CACTGCGACG 
GTTGCTGCAA AGACATCACA TGTGCAAATG GTGCCATCTG GTTCCATATT GTACTCGGTT 
GCTAGATACG ATGAAGATAT TGCTACTTAT GGAAACCGTG GGACAATCAC AGCTCGTGGA 
TTGTTGGAGC AGGTTAATGT TACACGAGAT ACAACTGATT ACCTGTGGTA CACAACCAGT 
GTGGATATTA AGGCATCAGA ATCATTCTTG CGTGGAGGAA AATGGCCAAC TCTTACAGTG 
GATTCTGCAG GGCATGCTGT TCATGTGTTT GTCAATGGAC ATTTTTACGG ATCTGCCTTT 
GGAACAAGAG AAAACAGAAA GTTTTCATTT AGCTCCCAAG TCAATCTACG AGGTGGAGCT 
AACAAAATCG CACTACTGAG TGTAGCAGTT GGGTTGCCGA ATGTTGGACC ACATTTTGAG 
ACGTGGGCCA CAGGAATCGT CGGGTCTGTG GTGCTTCATG GCCTTGACGA GGGTAACAAA 
GACTTGAGTT GGCAGAAATG GACTTATCAG GCTGGTCTGC GAGGGGAATC AATGAACTTG 
GTCTCTCCTA CTGAGGACTC CTCTGTTGAT TGGATCAAAG GCTCATTGGC TAAGCAAAAC 
AAACAACCTT TGACATGGTA CAAGGCCTAT TTTGACGCGC CTAGAGGGAA CGAGCCGCTG 
GCTTTGGATC TAAAGAGTAT GGGGAAAGGG CAAGCTTGGA TAAACGGGCA AAGCATAGGG 
AGATACTGGA TGGCATTTGC CAAAGGAGAC TGCGGAAGTT GTAACTACGC TGGAACATAC 
AGGCAGAACA AATGCCAGTC TGGTTGTGGC GAGCCGACAC AAAGATGGTA TCATGTTCCG 
CGTTCGTGGT TGAAGCCAAA AGGGAACTTG TTAGTACTTT TTGAAGAACT TGGTGGAGAT 
ATATCCAAAG TCTCTGTTGT GAAGAGATCA GTAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BGAL5 ( NM_001333243.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001320473.1 Arabidopsis thaliana beta-galactosidase 5 (BGAL5), mRNA.

Price & Availability↑

CloneID	OAb34213
Clone ID Related Accession (Same CDS sequence)	NM_001333243.1
Accession Version	NM_001333243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	beta-galactosidase 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGAGTGAAG TTGGTGGGGT TTTGTTTTTT TTATTGCAGT ACAATTTCGA AGGAAGATAT 
GATTTGGTAA GATTCATTAA GACGATTCAG GAAGTGGGTC TTTATGTTCA TCTCAGAATT 
GGTCCTTATG TTTGTGCAGA ATGGAATTTT GGAGGGTTTC CTGTGTGGTT GAAGTATGTA 
GATGGGATTA GTTTCAGAAC TGACAATGGA CCCTTCAAGT CTGCAATGCA AGGATTCACA 
GAGAAGATTG TTCAGATGAT GAAAGAACAT AGATTCTTTG CGTCACAAGG TGGACCTATC 
ATTCTTTCTC AGATTGAGAA TGAGTTTGAA CCAGATCTTA AAGGGCTTGG ACCGGCTGGT 
CACTCGTATG TCAATTGGGC TGCGAAAATG GCGGTCGGTT TAAACACGGG AGTACCGTGG 
GTGATGTGCA AGGAAGATGA TGCACCTGAC CCAATTATAA ACACTTGCAA CGGATTCTAC 
TGTGATTATT TTACTCCAAA TAAACCGTAT AAGCCAACTA TGTGGACAGA GGCATGGAGT 
GGCTGGTTCA CAGAGTTTGG TGGAACTGTT CCTAAACGAC CTGTAGAGGA TCTAGCATTT 
GGAGTAGCTC GTTTCATTCA AAAGGGCGGT TCGTATATAA ACTACTACAT GTACCATGGA 
GGAACAAACT TTGGACGCAC CGCAGGAGGT CCATTTATCA CCACTAGTTA TGACTATGAT 
GCTCCTATCG ATGAATACGG GTTGGTCCAA GAGCCCAAGT ACAGTCATCT TAAGCAGCTT 
CATCAGGCAA TCAAGCAATG TGAAGCTGCC TTAGTTTCTT CTGATCCACA TGTTACTAAA 
CTAGGAAACT ACGAGGAGGC TCATGTGTTC ACTGCTGGCA AAGGAAGTTG TGTAGCTTTC 
TTAACGAACT ATCACATGAA TGCACCTGCA AAAGTAGTGT TCAATAACCG ACACTATACT 
CTACCTGCTT GGTCCATCAG CATTCTTCCA GATTGTAGAA ACGTTGTTTT CAACACTGCG 
ACGGTTGCTG CAAAGACATC ACATGTGCAA ATGGTGCCAT CTGGTTCCAT ATTGTACTCG 
GTTGCTAGAT ACGATGAAGA TATTGCTACT TATGGAAACC GTGGGACAAT CACAGCTCGT 
GGATTGTTGG AGCAGGTTAA TGTTACACGA GATACAACTG ATTACCTGTG GTACACAACC 
AGTGTGGATA TTAAGGCATC AGAATCATTC TTGCGTGGAG GAAAATGGCC AACTCTTACA 
GTGGATTCTG CAGGGCATGC TGTTCATGTG TTTGTCAATG GACATTTTTA CGGATCTGCC 
TTTGGAACAA GAGAAAACAG AAAGTTTTCA TTTAGCTCCC AAGTCAATCT ACGAGGTGGA 
GCTAACAAAA TCGCACTACT GAGTGTAGCA GTTGGGTTGC CGAATGTTGG ACCACATTTT 
GAGACGTGGG CCACAGGAAT CGTCGGGTCT GTGGTGCTTC ATGGCCTTGA CGAGGGTAAC 
AAAGACTTGA GTTGGCAGAA ATGGACTTAT CAGGCTGGTC TGCGAGGGGA ATCAATGAAC 
TTGGTCTCTC CTACTGAGGA CTCCTCTGTT GATTGGATCA AAGGCTCATT GGCTAAGCAA 
AACAAACAAC CTTTGACATG GTACAAGGCC TATTTTGACG CGCCTAGAGG GAACGAGCCG 
CTGGCTTTGG ATCTAAAGAG TATGGGGAAA GGGCAAGCTT GGATAAACGG GCAAAGCATA 
GGGAGATACT GGATGGCATT TGCCAAAGGA GACTGCGGAA GTTGTAACTA CGCTGGAACA 
TACAGGCAGA ACAAATGCCA GTCTGGTTGT GGCGAGCCGA CACAAAGATG GTATCATGTT 
CCGCGTTCGT GGTTGAAGCC AAAAGGGAAC TTGTTAGTAC TTTTTGAAGA ACTTGGTGGA 
GATATATCCA AAGTCTCTGT TGTGAAGAGA TCAGTAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001320473.1
CDS	42..2003
Translation	MSEVGGVLFFLLQYNFEGRYDLVRFIKTIQEVGLYVHLRIGPYVCAEWNFGGFPVWLKYVDGISFRTDNGPFKSAMQGFTEKIVQMMKEHRFFASQGGPIILSQIENEFEPDLKGLGPAGHSYVNWAAKMAVGLNTGVPWVMCKEDDAPDPIINTCNGFYCDYFTPNKPYKPTMWTEAWSGWFTEFGGTVPKRPVEDLAFGVARFIQKGGSYINYYMYHGGTNFGRTAGGPFITTSYDYDAPIDEYGLVQEPKYSHLKQLHQAIKQCEAALVSSDPHVTKLGNYEEAHVFTAGKGSCVAFLTNYHMNAPAKVVFNNRHYTLPAWSISILPDCRNVVFNTATVAAKTSHVQMVPSGSILYSVARYDEDIATYGNRGTITARGLLEQVNVTRDTTDYLWYTTSVDIKASESFLRGGKWPTLTVDSAGHAVHVFVNGHFYGSAFGTRENRKFSFSSQVNLRGGANKIALLSVAVGLPNVGPHFETWATGIVGSVVLHGLDEGNKDLSWQKWTYQAGLRGESMNLVSPTEDSSVDWIKGSLAKQNKQPLTWYKAYFDAPRGNEPLALDLKSMGKGQAWINGQSIGRYWMAFAKGDCGSCNYAGTYRQNKCQSGCGEPTQRWYHVPRSWLKPKGNLLVLFEELGGDISKVSVVKRSVN

Target ORF information:

RefSeq Version	NM_001333243.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana beta-galactosidase 5 (BGAL5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333243.1

ORF Insert Sequence:

ATGAGTGAAG TTGGTGGGGT TTTGTTTTTT TTATTGCAGT ACAATTTCGA AGGAAGATAT 
GATTTGGTAA GATTCATTAA GACGATTCAG GAAGTGGGTC TTTATGTTCA TCTCAGAATT 
GGTCCTTATG TTTGTGCAGA ATGGAATTTT GGAGGGTTTC CTGTGTGGTT GAAGTATGTA 
GATGGGATTA GTTTCAGAAC TGACAATGGA CCCTTCAAGT CTGCAATGCA AGGATTCACA 
GAGAAGATTG TTCAGATGAT GAAAGAACAT AGATTCTTTG CGTCACAAGG TGGACCTATC 
ATTCTTTCTC AGATTGAGAA TGAGTTTGAA CCAGATCTTA AAGGGCTTGG ACCGGCTGGT 
CACTCGTATG TCAATTGGGC TGCGAAAATG GCGGTCGGTT TAAACACGGG AGTACCGTGG 
GTGATGTGCA AGGAAGATGA TGCACCTGAC CCAATTATAA ACACTTGCAA CGGATTCTAC 
TGTGATTATT TTACTCCAAA TAAACCGTAT AAGCCAACTA TGTGGACAGA GGCATGGAGT 
GGCTGGTTCA CAGAGTTTGG TGGAACTGTT CCTAAACGAC CTGTAGAGGA TCTAGCATTT 
GGAGTAGCTC GTTTCATTCA AAAGGGCGGT TCGTATATAA ACTACTACAT GTACCATGGA 
GGAACAAACT TTGGACGCAC CGCAGGAGGT CCATTTATCA CCACTAGTTA TGACTATGAT 
GCTCCTATCG ATGAATACGG GTTGGTCCAA GAGCCCAAGT ACAGTCATCT TAAGCAGCTT 
CATCAGGCAA TCAAGCAATG TGAAGCTGCC TTAGTTTCTT CTGATCCACA TGTTACTAAA 
CTAGGAAACT ACGAGGAGGC TCATGTGTTC ACTGCTGGCA AAGGAAGTTG TGTAGCTTTC 
TTAACGAACT ATCACATGAA TGCACCTGCA AAAGTAGTGT TCAATAACCG ACACTATACT 
CTACCTGCTT GGTCCATCAG CATTCTTCCA GATTGTAGAA ACGTTGTTTT CAACACTGCG 
ACGGTTGCTG CAAAGACATC ACATGTGCAA ATGGTGCCAT CTGGTTCCAT ATTGTACTCG 
GTTGCTAGAT ACGATGAAGA TATTGCTACT TATGGAAACC GTGGGACAAT CACAGCTCGT 
GGATTGTTGG AGCAGGTTAA TGTTACACGA GATACAACTG ATTACCTGTG GTACACAACC 
AGTGTGGATA TTAAGGCATC AGAATCATTC TTGCGTGGAG GAAAATGGCC AACTCTTACA 
GTGGATTCTG CAGGGCATGC TGTTCATGTG TTTGTCAATG GACATTTTTA CGGATCTGCC 
TTTGGAACAA GAGAAAACAG AAAGTTTTCA TTTAGCTCCC AAGTCAATCT ACGAGGTGGA 
GCTAACAAAA TCGCACTACT GAGTGTAGCA GTTGGGTTGC CGAATGTTGG ACCACATTTT 
GAGACGTGGG CCACAGGAAT CGTCGGGTCT GTGGTGCTTC ATGGCCTTGA CGAGGGTAAC 
AAAGACTTGA GTTGGCAGAA ATGGACTTAT CAGGCTGGTC TGCGAGGGGA ATCAATGAAC 
TTGGTCTCTC CTACTGAGGA CTCCTCTGTT GATTGGATCA AAGGCTCATT GGCTAAGCAA 
AACAAACAAC CTTTGACATG GTACAAGGCC TATTTTGACG CGCCTAGAGG GAACGAGCCG 
CTGGCTTTGG ATCTAAAGAG TATGGGGAAA GGGCAAGCTT GGATAAACGG GCAAAGCATA 
GGGAGATACT GGATGGCATT TGCCAAAGGA GACTGCGGAA GTTGTAACTA CGCTGGAACA 
TACAGGCAGA ACAAATGCCA GTCTGGTTGT GGCGAGCCGA CACAAAGATG GTATCATGTT 
CCGCGTTCGT GGTTGAAGCC AAAAGGGAAC TTGTTAGTAC TTTTTGAAGA ACTTGGTGGA 
GATATATCCA AAGTCTCTGT TGTGAAGAGA TCAGTAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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BGAL5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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