SS3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	SS3
Entrez Gene ID	837716
Full Name	starch synthase 3
Synonyms	ATSS3,F25C20.13,F25C20_13,STARCH SYNTHASE III,starch synthase 3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_101044.3	NP_172637.2	starch synthase 3
NM_001198036.2	NP_001184965.1	starch synthase 3

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	SS3	NP_001184965.1
Oryza sativa (rice)	Os04g0624600	NP_001053933.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

interaction of N-terminal starch-binding domains, particularly the D(316-344) and D(495-535) regions, with the catalytic domains, as well as the full integrity of the starch-binding capacity of the D2 domain, are involved in starch synthase III activity
Title: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III from Arabidopsis thaliana.

The data point to a complex mechanism for starch granule formation and to the different abilities of SSIV and SSIII to support this process in Arabidopsis leaves.
Title: Starch granule initiation in Arabidopsis requires the presence of either class IV or class III starch synthases.

SSII and SSIII have partially redundant functions. Loss of SSII caused increased amylose/amylopectin ratio, and loss of both SSII and SSIII caused slower plant growth and reduced starch content.
Title: Overlapping functions of the starch synthases SSII and SSIII in amylopectin biosynthesis in Arabidopsis.

Results presented here suggest that the N-terminal SBDs have a regulatory role, showing a starch binding capacity and modulating the catalytic properties of SSIII.
Title: Role of the N-terminal starch-binding domains in the kinetic properties of starch synthase III from Arabidopsis thaliana.

starch-synthase III (SSIII) contains a putative N-terminal transit peptide followed by a 557-amino acid SSIII-specific domain with 3 internal repeats & a C-terminal catalytic domain; each of the 3 internal repeats encodes a starch-binding domain
Title: Starch-synthase III family encodes a tandem of three starch-binding domains.

The following SS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb23261	NM_101044.3 Latest version!	Arabidopsis thaliana starch synthase 3 (SS3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb29452	NM_001198036.2 Latest version!	Arabidopsis thaliana starch synthase 3 (SS3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SS3 ( NM_101044.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_172637.2 Arabidopsis thaliana starch synthase 3 (SS3), mRNA.

Price & Availability↑

CloneID	OAb23261
Clone ID Related Accession (Same CDS sequence)	NM_101044.3
Accession Version	NM_101044.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	starch synthase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On May 26, 2011 this sequence version replaced NM_101044.2.

ATGATTTCTT ATTTTCTTAA CCAAGACTTT TCAAGGAAGA AGCAAGGAAG AATGGCTGCT 
TCAGGACCAA AAAGCTCAGG TCCCAGAGGT TTTGGGCGAC GAACAACAGT AGGAAGTGCT 
CAGAAAAGAA CTCAGAAGAA GAATGGTGAA AAAGATAGTA ATGCCACTTC TACAGCAACA 
AACGAGGTTT CAGGGATTAG TAAGTTGCCC GCAGCTAAAG TGGATGTACA GAAGCAAAGC 
TCTGTTGTTT TGAATGAGAG AAATGTGTTA GATAGGTCGG ATATTGAGGA TGGAAGTGAT 
CGTTTGGACA AGAAAACAAC CGATGATGAT GATTTGTTAG AACAAAAGTT AAAACTTGAA 
AGAGAGAATC TTCGTAGGAA GGAAATAGAA ACGCTTGCAG CGGAAAATTT GGCGAGAGGT 
GATAGAATGT TTGTGTATCC CGTTATTGTG AAACCTGATG AAGACATAGA AGTGTTTCTC 
AACAGGAATC TGTCGACTCT GAATAACGAA CCCGATGTTT TGATCATGGG GGCGTTTAAC 
GAATGGAGAT GGAAGTCTTT CACAAGGAGA TTGGAAAAGA CCTGGATCCA TGAAGATTGG 
TTGTCATGTC TCCTTCATAT CCCCAAAGAA GCGTATAAGA TGGACTTCGT GTTTTTCAAT 
GGGCAAAGTG TATATGACAA CAATGACTCA AAAGATTTTT GTGTAGAGAT AAAAGGTGGG 
ATGGATAAAG TTGACTTTGA GAATTTTCTT CTAGAAGAGA AACTGCGAGA GCAAGAGAAG 
TTAGCCAAGG AAGAAGCTGA GAGGGAGAGG CAAAAAGAAG AGAAGAGAAG AATCGAAGCT 
CAAAAGGCTG CAATTGAAGC TGATAGAGCA CAAGCAAAGG CGGAGACTCA GAAGAGACGT 
GAATTGCTTC AACCGGCTAT TAAGAAAGCT GTAGTCTCGG CTGAGAATGT TTGGTACATT 
GAGCCGAGTG ATTTCAAGGC TGAAGATACA GTGAAGCTAT ATTACAATAA AAGGTCAGGT 
CCTCTGACTA ATTCCAAAGA ACTGTGGTTA CATGGAGGGT TTAATAATTG GGTTGATGGA 
TTATCTATCG TTGTAAAGCT TGTTAATGCT GAGTTAAAGG ATGTTGATCC AAAGAGCGGA 
AATTGGTGGT TCGCTGAAGT TGTAGTGCCT GGCGGTGCAC TAGTCATTGA CTGGGTCTTT 
GCTGATGGAC CACCTAAAGG AGCGTTTCTG TATGACAATA ATGGTTACCA AGACTTCCAC 
GCACTTGTTC CTCAAAAACT TCCTGAAGAA CTTTACTGGT TAGAGGAAGA AAATATGATT 
TTTAGAAAAC TTCAGGAGGA TAGGCGGTTA AAAGAGGAAG TTATGCGTGC CAAGATGGAA 
AAAACAGCTC GCTTGAAAGC TGAAACTAAG GAAAGAACAC TGAAAAAGTT TCTGCTATCC 
CAGAAAGACG TGGTTTACAC CGAGCCTCTA GAGATTCAAG CAGGAAACCC TGTGACTGTA 
TTGTACAATC CTGCAAACAC GGTTTTGAAT GGAAAACCTG AAGTTTGGTT TAGAGGCTCT 
TTTAATCGTT GGACTCACCG CTTGGGCCCT TTGCCACCTC AGAAAATGGA AGCAACAGAT 
GATGAAAGCT CACATGTGAA GACTACGGCT AAGGTCCCAT TGGATGCTTA CATGATGGAC 
TTTGTGTTCT CTGAGAAAGA GGATGGCGGA ATATTTGATA ACAAAAATGG TCTGGATTAC 
CATTTACCAG TCGTGGGAGG TATTTCAAAG GAACCACCAT TGCACATTGT TCATATTGCT 
GTTGAAATGG CACCCATCGC AAAGGTTGGT GGCCTAGGTG ATGTTGTCAC TAGTCTATCT 
CGCGCTGTTC AAGAATTAAA CCATAATGTG GATATAGTTT TTCCAAAGTA TGATTGCATA 
AAGCACAATT TTGTGAAGGA CTTGCAATTT AACAGAAGCT ATCACTGGGG AGGAACTGAA 
ATAAAAGTTT GGCATGGAAA AGTAGAAGGC CTTTCGGTTT ACTTCTTAGA TCCACAAAAT 
GGATTGTTTC AGCGAGGATG TGTTTACGGT TGTGCAGATG ATGCAGGAAG ATTCGGTTTC 
TTCTGTCATG CGGCTCTTGA ATTTCTTCTC CAAGGAGGTT TCCATCCAGA CATTCTTCAC 
TGTCATGACT GGTCTAGTGC TCCGGTTTCA TGGTTATTCA AGGATCATTA CACACAGTAC 
GGTTTAATTA AAACCCGTAT TGTCTTCACA ATTCATAATT TGGAATTTGG AGCGAATGCC 
ATTGGTAAAG CAATGACATT TGCAGACAAA GCCACAACGG TTTCACCAAC TTATGCTAAG 
GAAGTTGCTG GAAACTCTGT AATCTCTGCA CATTTATACA AATTTCACGG AATTATAAAC 
GGGATTGACC CAGATATATG GGATCCATAT AACGATAACT TTATTCCCGT ACCTTATACT 
TCAGAGAACG TTGTAGAAGG CAAAAGAGCA GCCAAGGAAG AATTGCAAAA CAGGCTTGGA 
CTAAAGAGTG CCGATTTTCC AGTAGTAGGA ATTATTACGC GCTTAACACA CCAGAAGGGA 
ATACATTTGA TCAAGCACGC TATTTGGCGT ACCTTGGAAC GGAATGGACA GGTTGTCTTA 
TTAGGTTCAG CTCCAGATCC TCGGATCCAA AATGATTTTG TAAACTTGGC AAACCAATTA 
CATTCTTCTC ATGGTGACCG GGCTCGGCTT GTTCTAACCT ACGATGAACC TCTTTCCCAT 
TTGATTTATG CTGGGGCTGA CTTTATTCTT GTACCGTCGA TATTTGAGCC ATGTGGACTG 
ACACAGCTCA TAGCCATGAG ATACGGCGCT GTTCCTGTTG TTAGAAAAAC TGGAGGACTC 
TTTGATACGG TTTTTGATGT TGACCACGAT AAAGAAAGGG CACAAGCTCA AGTTCTAGAA 
CCTAATGGTT TCAGCTTCGA CGGAGCTGAT GCTCCTGGTG TTGATTATGC TCTCAATAGG 
GCGATATCGG CGTGGTACGA TGGTAGAGAG TGGTTTAACT CGCTGTGCAA GACGGTGATG 
GAGCAAGACT GGTCATGGAA CCGTCCTGCA CTTGAGTATC TTGAGCTCTA TCACTCTGCA 
CGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_172637.2
CDS	277..3405
Translation	MISYFLNQDFSRKKQGRMAASGPKSSGPRGFGRRTTVGSAQKRTQKKNGEKDSNATSTATNEVSGISKLPAAKVDVQKQSSVVLNERNVLDRSDIEDGSDRLDKKTTDDDDLLEQKLKLERENLRRKEIETLAAENLARGDRMFVYPVIVKPDEDIEVFLNRNLSTLNNEPDVLIMGAFNEWRWKSFTRRLEKTWIHEDWLSCLLHIPKEAYKMDFVFFNGQSVYDNNDSKDFCVEIKGGMDKVDFENFLLEEKLREQEKLAKEEAERERQKEEKRRIEAQKAAIEADRAQAKAETQKRRELLQPAIKKAVVSAENVWYIEPSDFKAEDTVKLYYNKRSGPLTNSKELWLHGGFNNWVDGLSIVVKLVNAELKDVDPKSGNWWFAEVVVPGGALVIDWVFADGPPKGAFLYDNNGYQDFHALVPQKLPEELYWLEEENMIFRKLQEDRRLKEEVMRAKMEKTARLKAETKERTLKKFLLSQKDVVYTEPLEIQAGNPVTVLYNPANTVLNGKPEVWFRGSFNRWTHRLGPLPPQKMEATDDESSHVKTTAKVPLDAYMMDFVFSEKEDGGIFDNKNGLDYHLPVVGGISKEPPLHIVHIAVEMAPIAKVGGLGDVVTSLSRAVQELNHNVDIVFPKYDCIKHNFVKDLQFNRSYHWGGTEIKVWHGKVEGLSVYFLDPQNGLFQRGCVYGCADDAGRFGFFCHAALEFLLQGGFHPDILHCHDWSSAPVSWLFKDHYTQYGLIKTRIVFTIHNLEFGANAIGKAMTFADKATTVSPTYAKEVAGNSVISAHLYKFHGIINGIDPDIWDPYNDNFIPVPYTSENVVEGKRAAKEELQNRLGLKSADFPVVGIITRLTHQKGIHLIKHAIWRTLERNGQVVLLGSAPDPRIQNDFVNLANQLHSSHGDRARLVLTYDEPLSHLIYAGADFILVPSIFEPCGLTQLIAMRYGAVPVVRKTGGLFDTVFDVDHDKERAQAQVLEPNGFSFDGADAPGVDYALNRAISAWYDGREWFNSLCKTVMEQDWSWNRPALEYLELYHSARK

Target ORF information:

RefSeq Version	NM_101044.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana starch synthase 3 (SS3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_101044.3

ORF Insert Sequence:

ATGATTTCTT ATTTTCTTAA CCAAGACTTT TCAAGGAAGA AGCAAGGAAG AATGGCTGCT 
TCAGGACCAA AAAGCTCAGG TCCCAGAGGT TTTGGGCGAC GAACAACAGT AGGAAGTGCT 
CAGAAAAGAA CTCAGAAGAA GAATGGTGAA AAAGATAGTA ATGCCACTTC TACAGCAACA 
AACGAGGTTT CAGGGATTAG TAAGTTGCCC GCAGCTAAAG TGGATGTACA GAAGCAAAGC 
TCTGTTGTTT TGAATGAGAG AAATGTGTTA GATAGGTCGG ATATTGAGGA TGGAAGTGAT 
CGTTTGGACA AGAAAACAAC CGATGATGAT GATTTGTTAG AACAAAAGTT AAAACTTGAA 
AGAGAGAATC TTCGTAGGAA GGAAATAGAA ACGCTTGCAG CGGAAAATTT GGCGAGAGGT 
GATAGAATGT TTGTGTATCC CGTTATTGTG AAACCTGATG AAGACATAGA AGTGTTTCTC 
AACAGGAATC TGTCGACTCT GAATAACGAA CCCGATGTTT TGATCATGGG GGCGTTTAAC 
GAATGGAGAT GGAAGTCTTT CACAAGGAGA TTGGAAAAGA CCTGGATCCA TGAAGATTGG 
TTGTCATGTC TCCTTCATAT CCCCAAAGAA GCGTATAAGA TGGACTTCGT GTTTTTCAAT 
GGGCAAAGTG TATATGACAA CAATGACTCA AAAGATTTTT GTGTAGAGAT AAAAGGTGGG 
ATGGATAAAG TTGACTTTGA GAATTTTCTT CTAGAAGAGA AACTGCGAGA GCAAGAGAAG 
TTAGCCAAGG AAGAAGCTGA GAGGGAGAGG CAAAAAGAAG AGAAGAGAAG AATCGAAGCT 
CAAAAGGCTG CAATTGAAGC TGATAGAGCA CAAGCAAAGG CGGAGACTCA GAAGAGACGT 
GAATTGCTTC AACCGGCTAT TAAGAAAGCT GTAGTCTCGG CTGAGAATGT TTGGTACATT 
GAGCCGAGTG ATTTCAAGGC TGAAGATACA GTGAAGCTAT ATTACAATAA AAGGTCAGGT 
CCTCTGACTA ATTCCAAAGA ACTGTGGTTA CATGGAGGGT TTAATAATTG GGTTGATGGA 
TTATCTATCG TTGTAAAGCT TGTTAATGCT GAGTTAAAGG ATGTTGATCC AAAGAGCGGA 
AATTGGTGGT TCGCTGAAGT TGTAGTGCCT GGCGGTGCAC TAGTCATTGA CTGGGTCTTT 
GCTGATGGAC CACCTAAAGG AGCGTTTCTG TATGACAATA ATGGTTACCA AGACTTCCAC 
GCACTTGTTC CTCAAAAACT TCCTGAAGAA CTTTACTGGT TAGAGGAAGA AAATATGATT 
TTTAGAAAAC TTCAGGAGGA TAGGCGGTTA AAAGAGGAAG TTATGCGTGC CAAGATGGAA 
AAAACAGCTC GCTTGAAAGC TGAAACTAAG GAAAGAACAC TGAAAAAGTT TCTGCTATCC 
CAGAAAGACG TGGTTTACAC CGAGCCTCTA GAGATTCAAG CAGGAAACCC TGTGACTGTA 
TTGTACAATC CTGCAAACAC GGTTTTGAAT GGAAAACCTG AAGTTTGGTT TAGAGGCTCT 
TTTAATCGTT GGACTCACCG CTTGGGCCCT TTGCCACCTC AGAAAATGGA AGCAACAGAT 
GATGAAAGCT CACATGTGAA GACTACGGCT AAGGTCCCAT TGGATGCTTA CATGATGGAC 
TTTGTGTTCT CTGAGAAAGA GGATGGCGGA ATATTTGATA ACAAAAATGG TCTGGATTAC 
CATTTACCAG TCGTGGGAGG TATTTCAAAG GAACCACCAT TGCACATTGT TCATATTGCT 
GTTGAAATGG CACCCATCGC AAAGGTTGGT GGCCTAGGTG ATGTTGTCAC TAGTCTATCT 
CGCGCTGTTC AAGAATTAAA CCATAATGTG GATATAGTTT TTCCAAAGTA TGATTGCATA 
AAGCACAATT TTGTGAAGGA CTTGCAATTT AACAGAAGCT ATCACTGGGG AGGAACTGAA 
ATAAAAGTTT GGCATGGAAA AGTAGAAGGC CTTTCGGTTT ACTTCTTAGA TCCACAAAAT 
GGATTGTTTC AGCGAGGATG TGTTTACGGT TGTGCAGATG ATGCAGGAAG ATTCGGTTTC 
TTCTGTCATG CGGCTCTTGA ATTTCTTCTC CAAGGAGGTT TCCATCCAGA CATTCTTCAC 
TGTCATGACT GGTCTAGTGC TCCGGTTTCA TGGTTATTCA AGGATCATTA CACACAGTAC 
GGTTTAATTA AAACCCGTAT TGTCTTCACA ATTCATAATT TGGAATTTGG AGCGAATGCC 
ATTGGTAAAG CAATGACATT TGCAGACAAA GCCACAACGG TTTCACCAAC TTATGCTAAG 
GAAGTTGCTG GAAACTCTGT AATCTCTGCA CATTTATACA AATTTCACGG AATTATAAAC 
GGGATTGACC CAGATATATG GGATCCATAT AACGATAACT TTATTCCCGT ACCTTATACT 
TCAGAGAACG TTGTAGAAGG CAAAAGAGCA GCCAAGGAAG AATTGCAAAA CAGGCTTGGA 
CTAAAGAGTG CCGATTTTCC AGTAGTAGGA ATTATTACGC GCTTAACACA CCAGAAGGGA 
ATACATTTGA TCAAGCACGC TATTTGGCGT ACCTTGGAAC GGAATGGACA GGTTGTCTTA 
TTAGGTTCAG CTCCAGATCC TCGGATCCAA AATGATTTTG TAAACTTGGC AAACCAATTA 
CATTCTTCTC ATGGTGACCG GGCTCGGCTT GTTCTAACCT ACGATGAACC TCTTTCCCAT 
TTGATTTATG CTGGGGCTGA CTTTATTCTT GTACCGTCGA TATTTGAGCC ATGTGGACTG 
ACACAGCTCA TAGCCATGAG ATACGGCGCT GTTCCTGTTG TTAGAAAAAC TGGAGGACTC 
TTTGATACGG TTTTTGATGT TGACCACGAT AAAGAAAGGG CACAAGCTCA AGTTCTAGAA 
CCTAATGGTT TCAGCTTCGA CGGAGCTGAT GCTCCTGGTG TTGATTATGC TCTCAATAGG 
GCGATATCGG CGTGGTACGA TGGTAGAGAG TGGTTTAACT CGCTGTGCAA GACGGTGATG 
GAGCAAGACT GGTCATGGAA CCGTCCTGCA CTTGAGTATC TTGAGCTCTA TCACTCTGCA 
CGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SS3 ( NM_001198036.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001184965.1 Arabidopsis thaliana starch synthase 3 (SS3), mRNA.

Price & Availability↑

CloneID	OAb29452
Clone ID Related Accession (Same CDS sequence)	NM_001198036.2
Accession Version	NM_001198036.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	starch synthase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_001198036.1.

ATGGAAGTGT GTTGGCAGAT ACAGAGACCA GCGAGTCTCA GACCGGGTTT TCAGGAAAAG 
GGTCGGGTCA AGATCAATAC TTTTTCTGTT GGGTTTTTTC CTCGCTCCAT TTCTAGTATT 
GCTTCATTGG AAAGTCAATA CAAATCAAAT GGGTTTCTGC ATCAGATCAC GGCAAGTGCA 
GACTTTTCAA GGAAGAAGCA AGGAAGAATG GCTGCTTCAG GACCAAAAAG CTCAGGTCCC 
AGAGGTTTTG GGCGACGAAC AACAGTAGGA AGTGCTCAGA AAAGAACTCA GAAGAAGAAT 
GGTGAAAAAG ATAGTAATGC CACTTCTACA GCAACAAACG AGGTTTCAGG GATTAGTAAG 
TTGCCCGCAG CTAAAGTGGA TGTACAGAAG CAAAGCTCTG TTGTTTTGAA TGAGAGAAAT 
GTGTTAGATA GGTCGGATAT TGAGGATGGA AGTGATCGTT TGGACAAGAA AACAACCGAT 
GATGATGATT TGTTAGAACA AAAGTTAAAA CTTGAAAGAG AGAATCTTCG TAGGAAGGAA 
ATAGAAACGC TTGCAGCGGA AAATTTGGCG AGAGGTGATA GAATGTTTGT GTATCCCGTT 
ATTGTGAAAC CTGATGAAGA CATAGAAGTG TTTCTCAACA GGAATCTGTC GACTCTGAAT 
AACGAACCCG ATGTTTTGAT CATGGGGGCG TTTAACGAAT GGAGATGGAA GTCTTTCACA 
AGGAGATTGG AAAAGACCTG GATCCATGAA GATTGGTTGT CATGTCTCCT TCATATCCCC 
AAAGAAGCGT ATAAGATGGA CTTCGTGTTT TTCAATGGGC AAAGTGTATA TGACAACAAT 
GACTCAAAAG ATTTTTGTGT AGAGATAAAA GGTGGGATGG ATAAAGTTGA CTTTGAGAAT 
TTTCTTCTAG AAGAGAAACT GCGAGAGCAA GAGAAGTTAG CCAAGGAAGA AGCTGAGAGG 
GAGAGGCAAA AAGAAGAGAA GAGAAGAATC GAAGCTCAAA AGGCTGCAAT TGAAGCTGAT 
AGAGCACAAG CAAAGGCGGA GACTCAGAAG AGACGTGAAT TGCTTCAACC GGCTATTAAG 
AAAGCTGTAG TCTCGGCTGA GAATGTTTGG TACATTGAGC CGAGTGATTT CAAGGCTGAA 
GATACAGTGA AGCTATATTA CAATAAAAGG TCAGGTCCTC TGACTAATTC CAAAGAACTG 
TGGTTACATG GAGGGTTTAA TAATTGGGTT GATGGATTAT CTATCGTTGT AAAGCTTGTT 
AATGCTGAGT TAAAGGATGT TGATCCAAAG AGCGGAAATT GGTGGTTCGC TGAAGTTGTA 
GTGCCTGGCG GTGCACTAGT CATTGACTGG GTCTTTGCTG ATGGACCACC TAAAGGAGCG 
TTTCTGTATG ACAATAATGG TTACCAAGAC TTCCACGCAC TTGTTCCTCA AAAACTTCCT 
GAAGAACTTT ACTGGTTAGA GGAAGAAAAT ATGATTTTTA GAAAACTTCA GGAGGATAGG 
CGGTTAAAAG AGGAAGTTAT GCGTGCCAAG ATGGAAAAAA CAGCTCGCTT GAAAGCTGAA 
ACTAAGGAAA GAACACTGAA AAAGTTTCTG CTATCCCAGA AAGACGTGGT TTACACCGAG 
CCTCTAGAGA TTCAAGCAGG AAACCCTGTG ACTGTATTGT ACAATCCTGC AAACACGGTT 
TTGAATGGAA AACCTGAAGT TTGGTTTAGA GGCTCTTTTA ATCGTTGGAC TCACCGCTTG 
GGCCCTTTGC CACCTCAGAA AATGGAAGCA ACAGATGATG AAAGCTCACA TGTGAAGACT 
ACGGCTAAGG TCCCATTGGA TGCTTACATG ATGGACTTTG TGTTCTCTGA GAAAGAGGAT 
GGCGGAATAT TTGATAACAA AAATGGTCTG GATTACCATT TACCAGTCGT GGGAGGTATT 
TCAAAGGAAC CACCATTGCA CATTGTTCAT ATTGCTGTTG AAATGGCACC CATCGCAAAG 
GTTGGTGGCC TAGGTGATGT TGTCACTAGT CTATCTCGCG CTGTTCAAGA ATTAAACCAT 
AATGTGGATA TAGTTTTTCC AAAGTATGAT TGCATAAAGC ACAATTTTGT GAAGGACTTG 
CAATTTAACA GAAGCTATCA CTGGGGAGGA ACTGAAATAA AAGTTTGGCA TGGAAAAGTA 
GAAGGCCTTT CGGTTTACTT CTTAGATCCA CAAAATGGAT TGTTTCAGCG AGGATGTGTT 
TACGGTTGTG CAGATGATGC AGGAAGATTC GGTTTCTTCT GTCATGCGGC TCTTGAATTT 
CTTCTCCAAG GAGGTTTCCA TCCAGACATT CTTCACTGTC ATGACTGGTC TAGTGCTCCG 
GTTTCATGGT TATTCAAGGA TCATTACACA CAGTACGGTT TAATTAAAAC CCGTATTGTC 
TTCACAATTC ATAATTTGGA ATTTGGAGCG AATGCCATTG GTAAAGCAAT GACATTTGCA 
GACAAAGCCA CAACGGTTTC ACCAACTTAT GCTAAGGAAG TTGCTGGAAA CTCTGTAATC 
TCTGCACATT TATACAAATT TCACGGAATT ATAAACGGGA TTGACCCAGA TATATGGGAT 
CCATATAACG ATAACTTTAT TCCCGTACCT TATACTTCAG AGAACGTTGT AGAAGGCAAA 
AGAGCAGCCA AGGAAGAATT GCAAAACAGG CTTGGACTAA AGAGTGCCGA TTTTCCAGTA 
GTAGGAATTA TTACGCGCTT AACACACCAG AAGGGAATAC ATTTGATCAA GCACGCTATT 
TGGCGTACCT TGGAACGGAA TGGACAGGTT GTCTTATTAG GTTCAGCTCC AGATCCTCGG 
ATCCAAAATG ATTTTGTAAA CTTGGCAAAC CAATTACATT CTTCTCATGG TGACCGGGCT 
CGGCTTGTTC TAACCTACGA TGAACCTCTT TCCCATTTGA TTTATGCTGG GGCTGACTTT 
ATTCTTGTAC CGTCGATATT TGAGCCATGT GGACTGACAC AGCTCATAGC CATGAGATAC 
GGCGCTGTTC CTGTTGTTAG AAAAACTGGA GGACTCTTTG ATACGGTTTT TGATGTTGAC 
CACGATAAAG AAAGGGCACA AGCTCAAGTT CTAGAACCTA ATGGTTTCAG CTTCGACGGA 
GCTGATGCTC CTGGTGTTGA TTATGCTCTC AATAGGGCGA TATCGGCGTG GTACGATGGT 
AGAGAGTGGT TTAACTCGCT GTGCAAGACG GTGATGGAGC AAGACTGGTC ATGGAACCGT 
CCTGCACTTG AGTATCTTGA GCTCTATCAC TCTGCACGCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001184965.1
CDS	232..3516
Translation	MEVCWQIQRPASLRPGFQEKGRVKINTFSVGFFPRSISSIASLESQYKSNGFLHQITASADFSRKKQGRMAASGPKSSGPRGFGRRTTVGSAQKRTQKKNGEKDSNATSTATNEVSGISKLPAAKVDVQKQSSVVLNERNVLDRSDIEDGSDRLDKKTTDDDDLLEQKLKLERENLRRKEIETLAAENLARGDRMFVYPVIVKPDEDIEVFLNRNLSTLNNEPDVLIMGAFNEWRWKSFTRRLEKTWIHEDWLSCLLHIPKEAYKMDFVFFNGQSVYDNNDSKDFCVEIKGGMDKVDFENFLLEEKLREQEKLAKEEAERERQKEEKRRIEAQKAAIEADRAQAKAETQKRRELLQPAIKKAVVSAENVWYIEPSDFKAEDTVKLYYNKRSGPLTNSKELWLHGGFNNWVDGLSIVVKLVNAELKDVDPKSGNWWFAEVVVPGGALVIDWVFADGPPKGAFLYDNNGYQDFHALVPQKLPEELYWLEEENMIFRKLQEDRRLKEEVMRAKMEKTARLKAETKERTLKKFLLSQKDVVYTEPLEIQAGNPVTVLYNPANTVLNGKPEVWFRGSFNRWTHRLGPLPPQKMEATDDESSHVKTTAKVPLDAYMMDFVFSEKEDGGIFDNKNGLDYHLPVVGGISKEPPLHIVHIAVEMAPIAKVGGLGDVVTSLSRAVQELNHNVDIVFPKYDCIKHNFVKDLQFNRSYHWGGTEIKVWHGKVEGLSVYFLDPQNGLFQRGCVYGCADDAGRFGFFCHAALEFLLQGGFHPDILHCHDWSSAPVSWLFKDHYTQYGLIKTRIVFTIHNLEFGANAIGKAMTFADKATTVSPTYAKEVAGNSVISAHLYKFHGIINGIDPDIWDPYNDNFIPVPYTSENVVEGKRAAKEELQNRLGLKSADFPVVGIITRLTHQKGIHLIKHAIWRTLERNGQVVLLGSAPDPRIQNDFVNLANQLHSSHGDRARLVLTYDEPLSHLIYAGADFILVPSIFEPCGLTQLIAMRYGAVPVVRKTGGLFDTVFDVDHDKERAQAQVLEPNGFSFDGADAPGVDYALNRAISAWYDGREWFNSLCKTVMEQDWSWNRPALEYLELYHSARK

Target ORF information:

RefSeq Version	NM_001198036.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana starch synthase 3 (SS3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198036.2

ORF Insert Sequence:

ATGGAAGTGT GTTGGCAGAT ACAGAGACCA GCGAGTCTCA GACCGGGTTT TCAGGAAAAG 
GGTCGGGTCA AGATCAATAC TTTTTCTGTT GGGTTTTTTC CTCGCTCCAT TTCTAGTATT 
GCTTCATTGG AAAGTCAATA CAAATCAAAT GGGTTTCTGC ATCAGATCAC GGCAAGTGCA 
GACTTTTCAA GGAAGAAGCA AGGAAGAATG GCTGCTTCAG GACCAAAAAG CTCAGGTCCC 
AGAGGTTTTG GGCGACGAAC AACAGTAGGA AGTGCTCAGA AAAGAACTCA GAAGAAGAAT 
GGTGAAAAAG ATAGTAATGC CACTTCTACA GCAACAAACG AGGTTTCAGG GATTAGTAAG 
TTGCCCGCAG CTAAAGTGGA TGTACAGAAG CAAAGCTCTG TTGTTTTGAA TGAGAGAAAT 
GTGTTAGATA GGTCGGATAT TGAGGATGGA AGTGATCGTT TGGACAAGAA AACAACCGAT 
GATGATGATT TGTTAGAACA AAAGTTAAAA CTTGAAAGAG AGAATCTTCG TAGGAAGGAA 
ATAGAAACGC TTGCAGCGGA AAATTTGGCG AGAGGTGATA GAATGTTTGT GTATCCCGTT 
ATTGTGAAAC CTGATGAAGA CATAGAAGTG TTTCTCAACA GGAATCTGTC GACTCTGAAT 
AACGAACCCG ATGTTTTGAT CATGGGGGCG TTTAACGAAT GGAGATGGAA GTCTTTCACA 
AGGAGATTGG AAAAGACCTG GATCCATGAA GATTGGTTGT CATGTCTCCT TCATATCCCC 
AAAGAAGCGT ATAAGATGGA CTTCGTGTTT TTCAATGGGC AAAGTGTATA TGACAACAAT 
GACTCAAAAG ATTTTTGTGT AGAGATAAAA GGTGGGATGG ATAAAGTTGA CTTTGAGAAT 
TTTCTTCTAG AAGAGAAACT GCGAGAGCAA GAGAAGTTAG CCAAGGAAGA AGCTGAGAGG 
GAGAGGCAAA AAGAAGAGAA GAGAAGAATC GAAGCTCAAA AGGCTGCAAT TGAAGCTGAT 
AGAGCACAAG CAAAGGCGGA GACTCAGAAG AGACGTGAAT TGCTTCAACC GGCTATTAAG 
AAAGCTGTAG TCTCGGCTGA GAATGTTTGG TACATTGAGC CGAGTGATTT CAAGGCTGAA 
GATACAGTGA AGCTATATTA CAATAAAAGG TCAGGTCCTC TGACTAATTC CAAAGAACTG 
TGGTTACATG GAGGGTTTAA TAATTGGGTT GATGGATTAT CTATCGTTGT AAAGCTTGTT 
AATGCTGAGT TAAAGGATGT TGATCCAAAG AGCGGAAATT GGTGGTTCGC TGAAGTTGTA 
GTGCCTGGCG GTGCACTAGT CATTGACTGG GTCTTTGCTG ATGGACCACC TAAAGGAGCG 
TTTCTGTATG ACAATAATGG TTACCAAGAC TTCCACGCAC TTGTTCCTCA AAAACTTCCT 
GAAGAACTTT ACTGGTTAGA GGAAGAAAAT ATGATTTTTA GAAAACTTCA GGAGGATAGG 
CGGTTAAAAG AGGAAGTTAT GCGTGCCAAG ATGGAAAAAA CAGCTCGCTT GAAAGCTGAA 
ACTAAGGAAA GAACACTGAA AAAGTTTCTG CTATCCCAGA AAGACGTGGT TTACACCGAG 
CCTCTAGAGA TTCAAGCAGG AAACCCTGTG ACTGTATTGT ACAATCCTGC AAACACGGTT 
TTGAATGGAA AACCTGAAGT TTGGTTTAGA GGCTCTTTTA ATCGTTGGAC TCACCGCTTG 
GGCCCTTTGC CACCTCAGAA AATGGAAGCA ACAGATGATG AAAGCTCACA TGTGAAGACT 
ACGGCTAAGG TCCCATTGGA TGCTTACATG ATGGACTTTG TGTTCTCTGA GAAAGAGGAT 
GGCGGAATAT TTGATAACAA AAATGGTCTG GATTACCATT TACCAGTCGT GGGAGGTATT 
TCAAAGGAAC CACCATTGCA CATTGTTCAT ATTGCTGTTG AAATGGCACC CATCGCAAAG 
GTTGGTGGCC TAGGTGATGT TGTCACTAGT CTATCTCGCG CTGTTCAAGA ATTAAACCAT 
AATGTGGATA TAGTTTTTCC AAAGTATGAT TGCATAAAGC ACAATTTTGT GAAGGACTTG 
CAATTTAACA GAAGCTATCA CTGGGGAGGA ACTGAAATAA AAGTTTGGCA TGGAAAAGTA 
GAAGGCCTTT CGGTTTACTT CTTAGATCCA CAAAATGGAT TGTTTCAGCG AGGATGTGTT 
TACGGTTGTG CAGATGATGC AGGAAGATTC GGTTTCTTCT GTCATGCGGC TCTTGAATTT 
CTTCTCCAAG GAGGTTTCCA TCCAGACATT CTTCACTGTC ATGACTGGTC TAGTGCTCCG 
GTTTCATGGT TATTCAAGGA TCATTACACA CAGTACGGTT TAATTAAAAC CCGTATTGTC 
TTCACAATTC ATAATTTGGA ATTTGGAGCG AATGCCATTG GTAAAGCAAT GACATTTGCA 
GACAAAGCCA CAACGGTTTC ACCAACTTAT GCTAAGGAAG TTGCTGGAAA CTCTGTAATC 
TCTGCACATT TATACAAATT TCACGGAATT ATAAACGGGA TTGACCCAGA TATATGGGAT 
CCATATAACG ATAACTTTAT TCCCGTACCT TATACTTCAG AGAACGTTGT AGAAGGCAAA 
AGAGCAGCCA AGGAAGAATT GCAAAACAGG CTTGGACTAA AGAGTGCCGA TTTTCCAGTA 
GTAGGAATTA TTACGCGCTT AACACACCAG AAGGGAATAC ATTTGATCAA GCACGCTATT 
TGGCGTACCT TGGAACGGAA TGGACAGGTT GTCTTATTAG GTTCAGCTCC AGATCCTCGG 
ATCCAAAATG ATTTTGTAAA CTTGGCAAAC CAATTACATT CTTCTCATGG TGACCGGGCT 
CGGCTTGTTC TAACCTACGA TGAACCTCTT TCCCATTTGA TTTATGCTGG GGCTGACTTT 
ATTCTTGTAC CGTCGATATT TGAGCCATGT GGACTGACAC AGCTCATAGC CATGAGATAC 
GGCGCTGTTC CTGTTGTTAG AAAAACTGGA GGACTCTTTG ATACGGTTTT TGATGTTGAC 
CACGATAAAG AAAGGGCACA AGCTCAAGTT CTAGAACCTA ATGGTTTCAG CTTCGACGGA 
GCTGATGCTC CTGGTGTTGA TTATGCTCTC AATAGGGCGA TATCGGCGTG GTACGATGGT 
AGAGAGTGGT TTAACTCGCT GTGCAAGACG GTGATGGAGC AAGACTGGTC ATGGAACCGT 
CCTGCACTTG AGTATCTTGA GCTCTATCAC TCTGCACGCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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SS3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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