PI-4KBETA1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PI-4KBETA1
Entrez Gene ID	836528
Full Name	phosphatidylinositol 4-OH kinase beta1
Synonyms	MHJ24.5,MHJ24_5,PHOSPHATIDYLINOSITOL 4-OH KINASE BETA1,PI4KBETA1,phosphatidylinositol 4-OH kinase beta1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_125803.4	NP_201212.1	phosphatidylinositol 4-OH kinase beta1
NM_001345618.1	NP_001330874.1	phosphatidylinositol 4-OH kinase beta1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	PI-4KBETA2	NP_196497.1
Arabidopsis thaliana (thale cress)	PI-4KBETA1	NP_201212.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PI4KIIIbeta1 and PI4KIIIbeta2 are genetically upstream of EDS1 and need functional salicylic acid biosynthesis and perception through NPR1 to express the dwarf phenotype. [PI4KIIIbeta1]
Title: Constitutive salicylic acid accumulation in pi4kIII?1?2 Arabidopsis plants stunts rosette but not root growth.

pi4kIIIbeta1beta2 double mutant stunted rosette is due to high salicylic acid, while the short root length is not.
Title: The Arabidopsis pi4kIII?1?2 double mutant is salicylic acid-overaccumulating: a new example of salicylic acid influence on plant stature.

AtPI4KIIIbeta1 and AtPI4KIIIbeta2 can both act upstream of the PI-PLC.
Title: Arabidopsis type-III phosphatidylinositol 4-kinases ?1 and ?2 are upstream of the phospholipase C pathway triggered by cold exposure.

RabA4b and PI-4Kbeta1 localize to budding secretory vesicles (SVs) in the trans Golgi network and to SVs en route to the cell surface.
Title: Electron tomography of RabA4b- and PI-4K?1-labeled trans Golgi network compartments in Arabidopsis.

PI-4Kbeta1 interacts with RabA4b through a novel homology domain, and interacts with a Ca2+ sensor through its NH2 terminus.
Title: A role for the RabA4b effector protein PI-4Kbeta1 in polarized expansion of root hair cells in Arabidopsis thaliana.

The following PI-4KBETA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PI-4KBETA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb42187	NM_001345618.1 Latest version!	Arabidopsis thaliana phosphatidylinositol 4-OH kinase beta1 (PI-4KBETA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAb22363	NM_125803.4 Latest version!	Arabidopsis thaliana phosphatidylinositol 4-OH kinase beta1 (PI-4KBETA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PI-4KBETA1 ( NM_001345618.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001330874.1 Arabidopsis thaliana phosphatidylinositol 4-OH kinase beta1 (PI-4KBETA1), mRNA.

Price & Availability↑

CloneID	OAb42187
Clone ID Related Accession (Same CDS sequence)	NM_001345618.1
Accession Version	NM_001345618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	phosphatidylinositol 4-OH kinase beta1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGCCGATGG GACGCTTTCT ATCTTTGGTC CGAGGAGACT CGGCTGAGTC ACCTCGTGAG 
ATTACCTCAC AGAGTAATAT CATCGGCGAT ACTGGTTCTA ATGGTTGGCT TATTAGGTTT 
TTCGATTCTG CTTTCTTCTG TGAGTGGATT GCTGTTAGTT ATCTGTACAA GCACCCTCAT 
GCTGGTGTTA GGGATTACTT ATGCAATAGG ATGTATACGC TTCCCTTATC AGGTATTGAG 
AGTTATCTCT TCCAGATTTG CTACATGATG GTGCACAAAC CAAGCCCATC GCTTGATAAG 
TTTGTTATAG ACATCTGTGG AAAGTCACTG AAAATCGCAC TGAAAGTGCA TTGGTTTTTG 
TTGGCTGAGC TGGAGGATGC TGACGATAAT GAAGGCATCA GCAGGATTCA AGAGAAGTGT 
CAAATTGCAG CTACTTTGAT GGGTGAGTGG TCTCCTCTCA TGCGTCCACA GAACGAGGTC 
TCAACCCCAG GGAGCAAGAA TCAGGTCCTA AATAGACTTT TGTCATCAAA ACAGAAGCTC 
TTCTCCTTAA AGCTATCTCC TCCCACCCAG AAGTCTTTGT CATTCTCACC TTCGCCAGGG 
ACCAACGTGC AGGATGATGG TAGTCAGTTA CCTGCTGAGG ACAATAAGAT TTTTAAGAAA 
CTTATCCCAA GTCCTAAAGT CAGAGATGCT TTAATGTTTA GAAAGTCAGC TGACAAAGAT 
GATGAAGAGA GTGAAAAGGA AGGATTCTTC AAGAGGCTCT TGAGGGACAG CAAAGGTGAG 
GGTGATGAAC CAATACCTAA CTCAGAGGGT TTCTTTAAGC GCCTTTTGAA AGACAATAAA 
TCAGAAGATG AGGACATAAC AAACAGTTCC GAGGGGTTCT TCAAGAGGCT CTTGAGTAGT 
AAAGGAGAAA GCGAGGAATT GACATCGAGT TCAGATGGGC TGTTTAAAAG GTTGTTACGA 
GATAATAAGG GAGATGAAGA GGAATTGGGT GCAAATTCAG ACAGTTTCTT CAAGAGATTG 
TTACGGGAGA GTAAAAACGA GGACGAGGAG TCAAACCCAA ACTCAGAAGG TTTTTTTAAG 
AAATTATTCC GTGACAGCAA ACCTGAAGAT GATAAAGTTC CTAAAGAAGT GGATGATGAG 
GACAAAGATG GGTTCCTTAA GAAACTTTTC AGGGAAAAAA ATGATGACAA AAGACATGGT 
AGTGAAAAGA ATGAAGCCAA TGGAACAGTG TATGCCGATA AGAAATCTGG TGAAGAGGAT 
GAGAGGGAGG GGTTTTTCAA AAAATTCTTT AAGGAAAAAT CTGATGACAA GAAAGACATT 
GTCAAGGTTG ATGACGGGAA TGAAAGTGAG GGTGATGAAT CTCCAGAATT TTCTCTTTTC 
AAAAGATTAT TCCGTATACA TCCCGAAGAT GCAAAACCTA CTTCAGAAAA TGAAAATAGC 
AGCAATGGCT TAGTTGAAAG CAGTCCTGGG ACAGAAAACT TTTTCCGCAA GTTGTTCAGA 
GATCGTGACC AATCTGTTGA AGATTCTGAA CTGTTTGGAT CAAAGAAACA CAAGGAGAAA 
CGGCCAGGCT CACCTAAACA GCGGGATGAT ACTCCATCTG GTAAGCCCCC GCTACCAAAC 
AATACTGCAT CACAATTCAG GAAAGGGGCT TACCACGAGT CGTTGGAGTT TGTACAAGCG 
TTATGTGAAA CGTCATATGG TTTGGTAGAT ATCTTTCCCA TCGAAGATCG GAAGATTGGT 
CTTCGGGAGT CTCTTGCAGA GATCAATTTT CATTTATCGG AAGCTGAAAT CACTGGAGGA 
ATTTGTTTTC CAATGGGGAG AGGAGTTTTT CGTGTGGTCC ATATTCCTGA AGACGAATGC 
ATTCTTTTGA ATTCTAGGGA AAAGGCGCCA TACATGATCT CCGTAGAAGT TTTGAAAGCA 
GAAACACCCA GTGCTAAAGA ATCATCGAAC TCCCAGAAGC TTTCTAAAGG GGGGATTCCA 
TTGGCAAACG GGGACGCATT CTTGCAAAAG CCACCTCCAT GGGCTTATCC ACTATGGACT 
ACTCAGGAGG TTTATCGCAA CAGTGCTGAC CGAATGTCGT TATCCACTGC TCAAGCAATT 
GATCAAGCGA TGACTCCTAA GTCAGAGGTG AAGGTGAAAC TTGTCAATGT AAGTCTCTCA 
GTGGAGGATC GTACTTCAGC TCTTGAATCA TTTGGCGATC CAATTGATGA TGTTCTGGGG 
GAAGCCCCAA GGACTGGGCT GAACAATGAT CTTGAATGGG TAAGGGTGGT CGTAACGGCT 
GACCCAGGAC TTAGAATGGA AAGCATTCCT GATCCATCAG TCCCACGTAA AAAGGAACAT 
CGTCGTGTTC CAAGTACTGT TGCCATGGAG GAAGTTAGGG CTGCTGCAGC GAAGGGAGAG 
GCACCTCCAG GCCTTCCTCT CAAAGGGGCG GGTCAGGATT CGTCGGATGC ACAACCAAGG 
GCCAATGGTG GAATGCTGAA GGAAGGTGAT GCCTTATCAG GTGAACTTTG GGAGGGAAAG 
CGAGACAGAA TACGTAAAGC TTCAATATAT GGAAAGTTAC CTGGTTGGGA CCTGCGTTCT 
AATCGGTATA TGTTTTGGGC CTTGAAGGCC TCGATCAATT TGATAGAGTA CTCTAAGGTT 
TCTGTTATTC GGGTTGATAC CGCTAATCTT CCTTGTCTGC AGACTTTCCA GAATGTTTAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001330874.1
CDS	545..3247
Translation	MPMGRFLSLVRGDSAESPREITSQSNIIGDTGSNGWLIRFFDSAFFCEWIAVSYLYKHPHAGVRDYLCNRMYTLPLSGIESYLFQICYMMVHKPSPSLDKFVIDICGKSLKIALKVHWFLLAELEDADDNEGISRIQEKCQIAATLMGEWSPLMRPQNEVSTPGSKNQVLNRLLSSKQKLFSLKLSPPTQKSLSFSPSPGTNVQDDGSQLPAEDNKIFKKLIPSPKVRDALMFRKSADKDDEESEKEGFFKRLLRDSKGEGDEPIPNSEGFFKRLLKDNKSEDEDITNSSEGFFKRLLSSKGESEELTSSSDGLFKRLLRDNKGDEEELGANSDSFFKRLLRESKNEDEESNPNSEGFFKKLFRDSKPEDDKVPKEVDDEDKDGFLKKLFREKNDDKRHGSEKNEANGTVYADKKSGEEDEREGFFKKFFKEKSDDKKDIVKVDDGNESEGDESPEFSLFKRLFRIHPEDAKPTSENENSSNGLVESSPGTENFFRKLFRDRDQSVEDSELFGSKKHKEKRPGSPKQRDDTPSGKPPLPNNTASQFRKGAYHESLEFVQALCETSYGLVDIFPIEDRKIGLRESLAEINFHLSEAEITGGICFPMGRGVFRVVHIPEDECILLNSREKAPYMISVEVLKAETPSAKESSNSQKLSKGGIPLANGDAFLQKPPPWAYPLWTTQEVYRNSADRMSLSTAQAIDQAMTPKSEVKVKLVNVSLSVEDRTSALESFGDPIDDVLGEAPRTGLNNDLEWVRVVVTADPGLRMESIPDPSVPRKKEHRRVPSTVAMEEVRAAAAKGEAPPGLPLKGAGQDSSDAQPRANGGMLKEGDALSGELWEGKRDRIRKASIYGKLPGWDLRSNRYMFWALKASINLIEYSKVSVIRVDTANLPCLQTFQNVY

Target ORF information:

RefSeq Version	NM_001345618.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana phosphatidylinositol 4-OH kinase beta1 (PI-4KBETA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001345618.1

ORF Insert Sequence:

ATGCCGATGG GACGCTTTCT ATCTTTGGTC CGAGGAGACT CGGCTGAGTC ACCTCGTGAG 
ATTACCTCAC AGAGTAATAT CATCGGCGAT ACTGGTTCTA ATGGTTGGCT TATTAGGTTT 
TTCGATTCTG CTTTCTTCTG TGAGTGGATT GCTGTTAGTT ATCTGTACAA GCACCCTCAT 
GCTGGTGTTA GGGATTACTT ATGCAATAGG ATGTATACGC TTCCCTTATC AGGTATTGAG 
AGTTATCTCT TCCAGATTTG CTACATGATG GTGCACAAAC CAAGCCCATC GCTTGATAAG 
TTTGTTATAG ACATCTGTGG AAAGTCACTG AAAATCGCAC TGAAAGTGCA TTGGTTTTTG 
TTGGCTGAGC TGGAGGATGC TGACGATAAT GAAGGCATCA GCAGGATTCA AGAGAAGTGT 
CAAATTGCAG CTACTTTGAT GGGTGAGTGG TCTCCTCTCA TGCGTCCACA GAACGAGGTC 
TCAACCCCAG GGAGCAAGAA TCAGGTCCTA AATAGACTTT TGTCATCAAA ACAGAAGCTC 
TTCTCCTTAA AGCTATCTCC TCCCACCCAG AAGTCTTTGT CATTCTCACC TTCGCCAGGG 
ACCAACGTGC AGGATGATGG TAGTCAGTTA CCTGCTGAGG ACAATAAGAT TTTTAAGAAA 
CTTATCCCAA GTCCTAAAGT CAGAGATGCT TTAATGTTTA GAAAGTCAGC TGACAAAGAT 
GATGAAGAGA GTGAAAAGGA AGGATTCTTC AAGAGGCTCT TGAGGGACAG CAAAGGTGAG 
GGTGATGAAC CAATACCTAA CTCAGAGGGT TTCTTTAAGC GCCTTTTGAA AGACAATAAA 
TCAGAAGATG AGGACATAAC AAACAGTTCC GAGGGGTTCT TCAAGAGGCT CTTGAGTAGT 
AAAGGAGAAA GCGAGGAATT GACATCGAGT TCAGATGGGC TGTTTAAAAG GTTGTTACGA 
GATAATAAGG GAGATGAAGA GGAATTGGGT GCAAATTCAG ACAGTTTCTT CAAGAGATTG 
TTACGGGAGA GTAAAAACGA GGACGAGGAG TCAAACCCAA ACTCAGAAGG TTTTTTTAAG 
AAATTATTCC GTGACAGCAA ACCTGAAGAT GATAAAGTTC CTAAAGAAGT GGATGATGAG 
GACAAAGATG GGTTCCTTAA GAAACTTTTC AGGGAAAAAA ATGATGACAA AAGACATGGT 
AGTGAAAAGA ATGAAGCCAA TGGAACAGTG TATGCCGATA AGAAATCTGG TGAAGAGGAT 
GAGAGGGAGG GGTTTTTCAA AAAATTCTTT AAGGAAAAAT CTGATGACAA GAAAGACATT 
GTCAAGGTTG ATGACGGGAA TGAAAGTGAG GGTGATGAAT CTCCAGAATT TTCTCTTTTC 
AAAAGATTAT TCCGTATACA TCCCGAAGAT GCAAAACCTA CTTCAGAAAA TGAAAATAGC 
AGCAATGGCT TAGTTGAAAG CAGTCCTGGG ACAGAAAACT TTTTCCGCAA GTTGTTCAGA 
GATCGTGACC AATCTGTTGA AGATTCTGAA CTGTTTGGAT CAAAGAAACA CAAGGAGAAA 
CGGCCAGGCT CACCTAAACA GCGGGATGAT ACTCCATCTG GTAAGCCCCC GCTACCAAAC 
AATACTGCAT CACAATTCAG GAAAGGGGCT TACCACGAGT CGTTGGAGTT TGTACAAGCG 
TTATGTGAAA CGTCATATGG TTTGGTAGAT ATCTTTCCCA TCGAAGATCG GAAGATTGGT 
CTTCGGGAGT CTCTTGCAGA GATCAATTTT CATTTATCGG AAGCTGAAAT CACTGGAGGA 
ATTTGTTTTC CAATGGGGAG AGGAGTTTTT CGTGTGGTCC ATATTCCTGA AGACGAATGC 
ATTCTTTTGA ATTCTAGGGA AAAGGCGCCA TACATGATCT CCGTAGAAGT TTTGAAAGCA 
GAAACACCCA GTGCTAAAGA ATCATCGAAC TCCCAGAAGC TTTCTAAAGG GGGGATTCCA 
TTGGCAAACG GGGACGCATT CTTGCAAAAG CCACCTCCAT GGGCTTATCC ACTATGGACT 
ACTCAGGAGG TTTATCGCAA CAGTGCTGAC CGAATGTCGT TATCCACTGC TCAAGCAATT 
GATCAAGCGA TGACTCCTAA GTCAGAGGTG AAGGTGAAAC TTGTCAATGT AAGTCTCTCA 
GTGGAGGATC GTACTTCAGC TCTTGAATCA TTTGGCGATC CAATTGATGA TGTTCTGGGG 
GAAGCCCCAA GGACTGGGCT GAACAATGAT CTTGAATGGG TAAGGGTGGT CGTAACGGCT 
GACCCAGGAC TTAGAATGGA AAGCATTCCT GATCCATCAG TCCCACGTAA AAAGGAACAT 
CGTCGTGTTC CAAGTACTGT TGCCATGGAG GAAGTTAGGG CTGCTGCAGC GAAGGGAGAG 
GCACCTCCAG GCCTTCCTCT CAAAGGGGCG GGTCAGGATT CGTCGGATGC ACAACCAAGG 
GCCAATGGTG GAATGCTGAA GGAAGGTGAT GCCTTATCAG GTGAACTTTG GGAGGGAAAG 
CGAGACAGAA TACGTAAAGC TTCAATATAT GGAAAGTTAC CTGGTTGGGA CCTGCGTTCT 
AATCGGTATA TGTTTTGGGC CTTGAAGGCC TCGATCAATT TGATAGAGTA CTCTAAGGTT 
TCTGTTATTC GGGTTGATAC CGCTAATCTT CCTTGTCTGC AGACTTTCCA GAATGTTTAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI-4KBETA1 ( NM_125803.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_201212.1 Arabidopsis thaliana phosphatidylinositol 4-OH kinase beta1 (PI-4KBETA1), mRNA.

Price & Availability↑

CloneID	OAb22363
Clone ID Related Accession (Same CDS sequence)	NM_125803.4
Accession Version	NM_125803.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	phosphatidylinositol 4-OH kinase beta1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_125803.3.

ATGCCGATGG GACGCTTTCT ATCTTTGGTC CGAGGAGACT CGGCTGAGTC ACCTCGTGAG 
ATTACCTCAC AGAGTAATAT CATCGGCGAT ACTGGTTCTA ATGGTTGGCT TATTAGGTTT 
TTCGATTCTG CTTTCTTCTG TGAGTGGATT GCTGTTAGTT ATCTGTACAA GCACCCTCAT 
GCTGGTGTTA GGGATTACTT ATGCAATAGG ATGTATACGC TTCCCTTATC AGGTATTGAG 
AGTTATCTCT TCCAGATTTG CTACATGATG GTGCACAAAC CAAGCCCATC GCTTGATAAG 
TTTGTTATAG ACATCTGTGG AAAGTCACTG AAAATCGCAC TGAAAGTGCA TTGGTTTTTG 
TTGGCTGAGC TGGAGGATGC TGACGATAAT GAAGGCATCA GCAGGATTCA AGAGAAGTGT 
CAAATTGCAG CTACTTTGAT GGGTGAGTGG TCTCCTCTCA TGCGTCCACA GAACGAGGTC 
TCAACCCCAG GGAGCAAGAA TCAGGTCCTA AATAGACTTT TGTCATCAAA ACAGAAGCTC 
TTCTCCTTAA AGCTATCTCC TCCCACCCAG AAGTCTTTGT CATTCTCACC TTCGCCAGGG 
ACCAACGTGC AGGATGATGG TAGTCAGTTA CCTGCTGAGG ACAATAAGAT TTTTAAGAAA 
CTTATCCCAA GTCCTAAAGT CAGAGATGCT TTAATGTTTA GAAAGTCAGC TGACAAAGAT 
GATGAAGAGA GTGAAAAGGA AGGATTCTTC AAGAGGCTCT TGAGGGACAG CAAAGGTGAG 
GGTGATGAAC CAATACCTAA CTCAGAGGGT TTCTTTAAGC GCCTTTTGAA AGACAATAAA 
TCAGAAGATG AGGACATAAC AAACAGTTCC GAGGGGTTCT TCAAGAGGCT CTTGAGTAGT 
AAAGGAGAAA GCGAGGAATT GACATCGAGT TCAGATGGGC TGTTTAAAAG GTTGTTACGA 
GATAATAAGG GAGATGAAGA GGAATTGGGT GCAAATTCAG ACAGTTTCTT CAAGAGATTG 
TTACGGGAGA GTAAAAACGA GGACGAGGAG TCAAACCCAA ACTCAGAAGG TTTTTTTAAG 
AAATTATTCC GTGACAGCAA ACCTGAAGAT GATAAAGTTC CTAAAGAAGT GGATGATGAG 
GACAAAGATG GGTTCCTTAA GAAACTTTTC AGGGAAAAAA ATGATGACAA AAGACATGGT 
AGTGAAAAGA ATGAAGCCAA TGGAACAGTG TATGCCGATA AGAAATCTGG TGAAGAGGAT 
GAGAGGGAGG GGTTTTTCAA AAAATTCTTT AAGGAAAAAT CTGATGACAA GAAAGACATT 
GTCAAGGTTG ATGACGGGAA TGAAAGTGAG GGTGATGAAT CTCCAGAATT TTCTCTTTTC 
AAAAGATTAT TCCGTATACA TCCCGAAGAT GCAAAACCTA CTTCAGAAAA TGAAAATAGC 
AGCAATGGCT TAGTTGAAAG CAGTCCTGGG ACAGAAAACT TTTTCCGCAA GTTGTTCAGA 
GATCGTGACC AATCTGTTGA AGATTCTGAA CTGTTTGGAT CAAAGAAACA CAAGGAGAAA 
CGGCCAGGCT CACCTAAACA GCGGGATGAT ACTCCATCTG GTAAGCCCCC GCTACCAAAC 
AATACTGCAT CACAATTCAG GAAAGGGGCT TACCACGAGT CGTTGGAGTT TGTACAAGCG 
TTATGTGAAA CGTCATATGG TTTGGTAGAT ATCTTTCCCA TCGAAGATCG GAAGATTGGT 
CTTCGGGAGT CTCTTGCAGA GATCAATTTT CATTTATCGG AAGCTGAAAT CACTGGAGGA 
ATTTGTTTTC CAATGGGGAG AGGAGTTTTT CGTGTGGTCC ATATTCCTGA AGACGAATGC 
ATTCTTTTGA ATTCTAGGGA AAAGGCGCCA TACATGATCT CCGTAGAAGT TTTGAAAGCA 
GAAACACCCA GTGCTAAAGA ATCATCGAAC TCCCAGAAGC TTTCTAAAGG GGGGATTCCA 
TTGGCAAACG GGGACGCATT CTTGCAAAAG CCACCTCCAT GGGCTTATCC ACTATGGACT 
ACTCAGGAGG TTTATCGCAA CAGTGCTGAC CGAATGTCGT TATCCACTGC TCAAGCAATT 
GATCAAGCGA TGACTCCTAA GTCAGAGGTG AAGGTGAAAC TTGTCAATGT AAGTCTCTCA 
GTGGAGGATC GTACTTCAGC TCTTGAATCA TTTGGCGATC CAATTGATGA TGTTCTGGGG 
GAAGCCCCAA GGACTGGGCT GAACAATGAT CTTGAATGGG TAAGGGTGGT CGTAACGGCT 
GACCCAGGAC TTAGAATGGA AAGCATTCCT GATCCATCAG TCCCACGTAA AAAGGAACAT 
CGTCGTGTTC CAAGTACTGT TGCCATGGAG GAAGTTAGGG CTGCTGCAGC GAAGGGAGAG 
GCACCTCCAG GCCTTCCTCT CAAAGGGGCG GGTCAGGATT CGTCGGATGC ACAACCAAGG 
GCCAATGGTG GAATGCTGAA GGAAGGTGAT GCCTTATCAG GTGAACTTTG GGAGGGAAAG 
CGAGACAGAA TACGTAAAGC TTCAATATAT GGAAAGTTAC CTGGTTGGGA CCTGCGTTCT 
ATCATTGTGA AGAGCGGTGA CGACTGTAGG CAGGAGCATC TTGCTGTGCA ACTCATTTCT 
CACTTTTATG ATATATTCCA GGAAGCAGGT CTACCCCTCT GGTTACGTCC TTATGAAGTC 
TTGGTTACAT CTTCTTATAC TGCCCTTATA GAAACAATTC CAGATACGGC TTCTATTCAT 
TCTATCAAAA GTAGATATCC CAACATAACG AGCCTACGTG ATTTCTTTGT TGCAAAGTAT 
AAAGAAAACT CTCCAAGTTT TAAGCTCGCC CAGAGGAATT TCGTGGAAAG TATGGCCGGA 
TATTCTTTGG TCTGTTACCT TCTGCAGGTA AAAGATCGTC ATAATGGAAA TCTTCTCTTG 
GATGAAGAGG GTCACATTAT ACACATTGAT TTTGGTTTTA TGCTTTCGAA TTCTCCTGGT 
GGCGTAAACT TTGAGAGTGC CCCGTTTAAG TTAACCCGTG AACTTCTTGA GGTCATGGAC 
TCTGATGCTG ATGGGGTTCC AAGTGAGTTC TTTGACTATT TCAAGGTGCT ATGCATTCAA 
GGATTCCTTA CATGTAGAAA GCACGCGGAA CGGATTATTC TCTTAGTTGA AATGTTACAG 
GATTCGGGTT TCCCTTGCTT CAAAGGTGGT CCACGAACCA TTCAAAACCT AAGAAAAAGA 
TTCCATCTAA GTTTAACTGA AGAGCAATGT GTCTCGTTGG TCCTCTCTCT CATCAGCAGT 
AGCTTAGATG CTTGGCGGAC ACGACAATAT GATTACTACC AACGGGTCTT AAATGGAATA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_201212.1
CDS	577..3942
Translation	MPMGRFLSLVRGDSAESPREITSQSNIIGDTGSNGWLIRFFDSAFFCEWIAVSYLYKHPHAGVRDYLCNRMYTLPLSGIESYLFQICYMMVHKPSPSLDKFVIDICGKSLKIALKVHWFLLAELEDADDNEGISRIQEKCQIAATLMGEWSPLMRPQNEVSTPGSKNQVLNRLLSSKQKLFSLKLSPPTQKSLSFSPSPGTNVQDDGSQLPAEDNKIFKKLIPSPKVRDALMFRKSADKDDEESEKEGFFKRLLRDSKGEGDEPIPNSEGFFKRLLKDNKSEDEDITNSSEGFFKRLLSSKGESEELTSSSDGLFKRLLRDNKGDEEELGANSDSFFKRLLRESKNEDEESNPNSEGFFKKLFRDSKPEDDKVPKEVDDEDKDGFLKKLFREKNDDKRHGSEKNEANGTVYADKKSGEEDEREGFFKKFFKEKSDDKKDIVKVDDGNESEGDESPEFSLFKRLFRIHPEDAKPTSENENSSNGLVESSPGTENFFRKLFRDRDQSVEDSELFGSKKHKEKRPGSPKQRDDTPSGKPPLPNNTASQFRKGAYHESLEFVQALCETSYGLVDIFPIEDRKIGLRESLAEINFHLSEAEITGGICFPMGRGVFRVVHIPEDECILLNSREKAPYMISVEVLKAETPSAKESSNSQKLSKGGIPLANGDAFLQKPPPWAYPLWTTQEVYRNSADRMSLSTAQAIDQAMTPKSEVKVKLVNVSLSVEDRTSALESFGDPIDDVLGEAPRTGLNNDLEWVRVVVTADPGLRMESIPDPSVPRKKEHRRVPSTVAMEEVRAAAAKGEAPPGLPLKGAGQDSSDAQPRANGGMLKEGDALSGELWEGKRDRIRKASIYGKLPGWDLRSIIVKSGDDCRQEHLAVQLISHFYDIFQEAGLPLWLRPYEVLVTSSYTALIETIPDTASIHSIKSRYPNITSLRDFFVAKYKENSPSFKLAQRNFVESMAGYSLVCYLLQVKDRHNGNLLLDEEGHIIHIDFGFMLSNSPGGVNFESAPFKLTRELLEVMDSDADGVPSEFFDYFKVLCIQGFLTCRKHAERIILLVEMLQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLVLSLISSSLDAWRTRQYDYYQRVLNGIL

Target ORF information:

RefSeq Version	NM_125803.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana phosphatidylinositol 4-OH kinase beta1 (PI-4KBETA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_125803.4

ORF Insert Sequence:

ATGCCGATGG GACGCTTTCT ATCTTTGGTC CGAGGAGACT CGGCTGAGTC ACCTCGTGAG 
ATTACCTCAC AGAGTAATAT CATCGGCGAT ACTGGTTCTA ATGGTTGGCT TATTAGGTTT 
TTCGATTCTG CTTTCTTCTG TGAGTGGATT GCTGTTAGTT ATCTGTACAA GCACCCTCAT 
GCTGGTGTTA GGGATTACTT ATGCAATAGG ATGTATACGC TTCCCTTATC AGGTATTGAG 
AGTTATCTCT TCCAGATTTG CTACATGATG GTGCACAAAC CAAGCCCATC GCTTGATAAG 
TTTGTTATAG ACATCTGTGG AAAGTCACTG AAAATCGCAC TGAAAGTGCA TTGGTTTTTG 
TTGGCTGAGC TGGAGGATGC TGACGATAAT GAAGGCATCA GCAGGATTCA AGAGAAGTGT 
CAAATTGCAG CTACTTTGAT GGGTGAGTGG TCTCCTCTCA TGCGTCCACA GAACGAGGTC 
TCAACCCCAG GGAGCAAGAA TCAGGTCCTA AATAGACTTT TGTCATCAAA ACAGAAGCTC 
TTCTCCTTAA AGCTATCTCC TCCCACCCAG AAGTCTTTGT CATTCTCACC TTCGCCAGGG 
ACCAACGTGC AGGATGATGG TAGTCAGTTA CCTGCTGAGG ACAATAAGAT TTTTAAGAAA 
CTTATCCCAA GTCCTAAAGT CAGAGATGCT TTAATGTTTA GAAAGTCAGC TGACAAAGAT 
GATGAAGAGA GTGAAAAGGA AGGATTCTTC AAGAGGCTCT TGAGGGACAG CAAAGGTGAG 
GGTGATGAAC CAATACCTAA CTCAGAGGGT TTCTTTAAGC GCCTTTTGAA AGACAATAAA 
TCAGAAGATG AGGACATAAC AAACAGTTCC GAGGGGTTCT TCAAGAGGCT CTTGAGTAGT 
AAAGGAGAAA GCGAGGAATT GACATCGAGT TCAGATGGGC TGTTTAAAAG GTTGTTACGA 
GATAATAAGG GAGATGAAGA GGAATTGGGT GCAAATTCAG ACAGTTTCTT CAAGAGATTG 
TTACGGGAGA GTAAAAACGA GGACGAGGAG TCAAACCCAA ACTCAGAAGG TTTTTTTAAG 
AAATTATTCC GTGACAGCAA ACCTGAAGAT GATAAAGTTC CTAAAGAAGT GGATGATGAG 
GACAAAGATG GGTTCCTTAA GAAACTTTTC AGGGAAAAAA ATGATGACAA AAGACATGGT 
AGTGAAAAGA ATGAAGCCAA TGGAACAGTG TATGCCGATA AGAAATCTGG TGAAGAGGAT 
GAGAGGGAGG GGTTTTTCAA AAAATTCTTT AAGGAAAAAT CTGATGACAA GAAAGACATT 
GTCAAGGTTG ATGACGGGAA TGAAAGTGAG GGTGATGAAT CTCCAGAATT TTCTCTTTTC 
AAAAGATTAT TCCGTATACA TCCCGAAGAT GCAAAACCTA CTTCAGAAAA TGAAAATAGC 
AGCAATGGCT TAGTTGAAAG CAGTCCTGGG ACAGAAAACT TTTTCCGCAA GTTGTTCAGA 
GATCGTGACC AATCTGTTGA AGATTCTGAA CTGTTTGGAT CAAAGAAACA CAAGGAGAAA 
CGGCCAGGCT CACCTAAACA GCGGGATGAT ACTCCATCTG GTAAGCCCCC GCTACCAAAC 
AATACTGCAT CACAATTCAG GAAAGGGGCT TACCACGAGT CGTTGGAGTT TGTACAAGCG 
TTATGTGAAA CGTCATATGG TTTGGTAGAT ATCTTTCCCA TCGAAGATCG GAAGATTGGT 
CTTCGGGAGT CTCTTGCAGA GATCAATTTT CATTTATCGG AAGCTGAAAT CACTGGAGGA 
ATTTGTTTTC CAATGGGGAG AGGAGTTTTT CGTGTGGTCC ATATTCCTGA AGACGAATGC 
ATTCTTTTGA ATTCTAGGGA AAAGGCGCCA TACATGATCT CCGTAGAAGT TTTGAAAGCA 
GAAACACCCA GTGCTAAAGA ATCATCGAAC TCCCAGAAGC TTTCTAAAGG GGGGATTCCA 
TTGGCAAACG GGGACGCATT CTTGCAAAAG CCACCTCCAT GGGCTTATCC ACTATGGACT 
ACTCAGGAGG TTTATCGCAA CAGTGCTGAC CGAATGTCGT TATCCACTGC TCAAGCAATT 
GATCAAGCGA TGACTCCTAA GTCAGAGGTG AAGGTGAAAC TTGTCAATGT AAGTCTCTCA 
GTGGAGGATC GTACTTCAGC TCTTGAATCA TTTGGCGATC CAATTGATGA TGTTCTGGGG 
GAAGCCCCAA GGACTGGGCT GAACAATGAT CTTGAATGGG TAAGGGTGGT CGTAACGGCT 
GACCCAGGAC TTAGAATGGA AAGCATTCCT GATCCATCAG TCCCACGTAA AAAGGAACAT 
CGTCGTGTTC CAAGTACTGT TGCCATGGAG GAAGTTAGGG CTGCTGCAGC GAAGGGAGAG 
GCACCTCCAG GCCTTCCTCT CAAAGGGGCG GGTCAGGATT CGTCGGATGC ACAACCAAGG 
GCCAATGGTG GAATGCTGAA GGAAGGTGAT GCCTTATCAG GTGAACTTTG GGAGGGAAAG 
CGAGACAGAA TACGTAAAGC TTCAATATAT GGAAAGTTAC CTGGTTGGGA CCTGCGTTCT 
ATCATTGTGA AGAGCGGTGA CGACTGTAGG CAGGAGCATC TTGCTGTGCA ACTCATTTCT 
CACTTTTATG ATATATTCCA GGAAGCAGGT CTACCCCTCT GGTTACGTCC TTATGAAGTC 
TTGGTTACAT CTTCTTATAC TGCCCTTATA GAAACAATTC CAGATACGGC TTCTATTCAT 
TCTATCAAAA GTAGATATCC CAACATAACG AGCCTACGTG ATTTCTTTGT TGCAAAGTAT 
AAAGAAAACT CTCCAAGTTT TAAGCTCGCC CAGAGGAATT TCGTGGAAAG TATGGCCGGA 
TATTCTTTGG TCTGTTACCT TCTGCAGGTA AAAGATCGTC ATAATGGAAA TCTTCTCTTG 
GATGAAGAGG GTCACATTAT ACACATTGAT TTTGGTTTTA TGCTTTCGAA TTCTCCTGGT 
GGCGTAAACT TTGAGAGTGC CCCGTTTAAG TTAACCCGTG AACTTCTTGA GGTCATGGAC 
TCTGATGCTG ATGGGGTTCC AAGTGAGTTC TTTGACTATT TCAAGGTGCT ATGCATTCAA 
GGATTCCTTA CATGTAGAAA GCACGCGGAA CGGATTATTC TCTTAGTTGA AATGTTACAG 
GATTCGGGTT TCCCTTGCTT CAAAGGTGGT CCACGAACCA TTCAAAACCT AAGAAAAAGA 
TTCCATCTAA GTTTAACTGA AGAGCAATGT GTCTCGTTGG TCCTCTCTCT CATCAGCAGT 
AGCTTAGATG CTTGGCGGAC ACGACAATAT GATTACTACC AACGGGTCTT AAATGGAATA 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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PI-4KBETA1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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