CAD1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAD1
Entrez Gene ID	834430
Full Name	phytochelatin synthase 1 (PCS1)
Synonyms	ARA8,ARABIDOPSIS THALIANA PHYTOCHELATIN SYNTHASE 1,ATPCS1,CADMIUM SENSITIVE 1,MRH10.18,MRH10_18,PCS1,PHYTOCHELATIN SYNTHASE,PHYTOCHELATIN SYNTHASE 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_123774.4	NP_199220.1	phytochelatin synthase 1 (PCS1)
NM_001344531.1	NP_001332549.1	phytochelatin synthase 1 (PCS1)

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0102300	NP_001056534.1
Oryza sativa (rice)	Os05g0415200	NP_001055554.1
Arabidopsis thaliana (thale cress)	PCS2	NP_171894.1
Arabidopsis thaliana (thale cress)	CAD1	NP_199220.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings suggest that the PCS1 protein (At5g44070) might be involved in the production of the enzyme phytochelatin synthase, which in turn bind, localize, store or sequester heavy metals in plant cells.
Title: In silico and in vivo studies of molecular structures and mechanisms of AtPCS1 protein involved in binding arsenite and/or cadmium in plant cells.

Loss of AtNRAMP3 and AtNRAMP4 function or of PCS1 function leads to comparable Cd sensitivity.
Title: Mutants impaired in vacuolar metal mobilization identify chloroplasts as a target for cadmium hypersensitivity in Arabidopsis thaliana.

Recombinant AtPCS1s with mutations at Tyr55 showed lower catalytic activities because of reduced affinity (3-fold for Y55W) for the CdGS2, further suggesting the role of the cation-pi interaction in recognition of the second substrate.
Title: Tentative identification of the second substrate binding site in Arabidopsis phytochelatin synthase.

Inducible expression of the Arabidopsis phytochelatin synthase AtPCS1 in the triple knockout yeast system, resulted in the turnover of glutathione-S-bimane to the gamma-GluCys-bimane conjugate as observed in plants. [phytochelatin synthase] [AtPCS1]
Title: Dissection of glutathione conjugate turnover in yeast.

In contrast to wild type and CePCS transformants, AtPCS1 transformants were Cd-hypersensitive although there was no difference in cadmium accumulation between studied lines.
Title: Overexpression of phytochelatin synthase in tobacco: distinctive effects of AtPCS1 and CePCS genes on plant response to cadmium.

The following CAD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb20488	NM_123774.4 Latest version!	Arabidopsis thaliana phytochelatin synthase 1 (PCS1) (CAD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OAb41563	NM_001344531.1 Latest version!	Arabidopsis thaliana phytochelatin synthase 1 (PCS1) (CAD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAD1 ( NM_123774.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_199220.1 Arabidopsis thaliana phytochelatin synthase 1 (PCS1) (CAD1), mRNA.

Price & Availability↑

CloneID	OAb20488
Clone ID Related Accession (Same CDS sequence)	NM_123774.4
Accession Version	NM_123774.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	phytochelatin synthase 1 (PCS1)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_123774.3.

ATGGCTATGG CGAGTTTATA TCGGCGATCT CTTCCTTCTC CTCCGGCCAT TGACTTTTCT 
TCCGCCGAAG GCAAGCTAAT CTTCAATGAA GCGCTTCAGA AAGGAACTAT GGAAGGATTT 
TTCAGGTTGA TTTCGTATTT TCAGACACAA TCCGAACCTG CGTATTGTGG TTTGGCTAGT 
CTCTCAGTGG TGTTGAATGC TCTTTCTATC GATCCTGGAC GTAAATGGAA AGGGCCTTGG 
AGGTGGTTTG ATGAATCAAT GTTGGATTGC TGCGAACCTC TGGAAGTAGT GAAGGAAAAA 
GGCATTTCAT TTGGAAAAGT TGTCTGTTTG GCTCATTGTT CAGGAGCAAA AGTTGAGGCT 
TTCCGTACAA GTCAGAGCAC CATTGATGAT TTCCGCAAAT TTGTCGTCAA ATGCACGAGT 
TCTGAGAATT GTCATATGAT CTCAACATAT CACCGAGGTG TATTTAAGCA GACTGGGACT 
GGTCACTTTT CACCTATTGG TGGCTATAAT GCTGAGAGAG ATATGGCTTT GATTCTTGAT 
GTTGCTCGTT TCAAGTATCC CCCTCACTGG GTTCCTCTTA AACTTCTTTG GGAAGCCATG 
GACAGTATTG ATCAGTCAAC AGGGAAACGT AGAGGGTTCA TGCTCATATC TAGACCACAC 
AGAGAACCCG GATTGCTCTA TACTCTGAGC TGCAAGGATG AAAGCTGGAT CGAAATAGCC 
AAGTATTTGA AGGAAGATGT TCCTCGTCTT GTAAGTTCAC AGCATGTAGA TTCTGTGGAG 
AAAATCATAT CAGTTGTGTT CAAGTCACTT CCATCAAATT TCAACCAATT CATCAGATGG 
GTGGCTGAGA TCCGAATTAC AGAGGACTCA AACCAAAATC TCAGCGCAGA GGAGAAGTCT 
AGGCTGAAAC TAAAGCAATT GGTGCTGAAG GAAGTGCACG AAACTGAACT GTTCAAACAC 
ATCAATAAGT TCTTATCCAC AGTGGGTTAT GAAGACAGTC TGACTTATGC TGCTGCAAAG 
GCTTGTTGCC AAGGAGCTGA AATCTTATCC GGAAGCCCAT CAAAAGAGTT TTGTTGTCGG 
GAAACTTGCG TGAAATGCAT CAAAGGTCCT GATGACTCTG AAGGCACGGT GGTGACTGGA 
GTTGTGGTGC GTGATGGGAA TGAACAAAAG GTTGATCTGT TAGTGCCATC GACGCAAACT 
GAGTGTGAAT GTGGTCCTGA AGCAACTTAT CCAGCAGGAA ACGATGTGTT CACTGCACTT 
CTATTGGCTT TACCTCCACA GACATGGTCA GGGATCAAAG ACCAAGCTCT TATGCATGAA 
ATGAAGCAGC TCATTTCCAT GGCTTCCCTC CCAACTTTGC TTCAAGAAGA GGTATTGCAT 
CTTCGACGGC AACTTCAGCT GCTAAAACGA TGCCAAGAGA ACAAGGAAGA GGATGATCTC 
GCTGCTCCTG CCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_199220.1
CDS	171..1628
Translation	MAMASLYRRSLPSPPAIDFSSAEGKLIFNEALQKGTMEGFFRLISYFQTQSEPAYCGLASLSVVLNALSIDPGRKWKGPWRWFDESMLDCCEPLEVVKEKGISFGKVVCLAHCSGAKVEAFRTSQSTIDDFRKFVVKCTSSENCHMISTYHRGVFKQTGTGHFSPIGGYNAERDMALILDVARFKYPPHWVPLKLLWEAMDSIDQSTGKRRGFMLISRPHREPGLLYTLSCKDESWIEIAKYLKEDVPRLVSSQHVDSVEKIISVVFKSLPSNFNQFIRWVAEIRITEDSNQNLSAEEKSRLKLKQLVLKEVHETELFKHINKFLSTVGYEDSLTYAAAKACCQGAEILSGSPSKEFCCRETCVKCIKGPDDSEGTVVTGVVVRDGNEQKVDLLVPSTQTECECGPEATYPAGNDVFTALLLALPPQTWSGIKDQALMHEMKQLISMASLPTLLQEEVLHLRRQLQLLKRCQENKEEDDLAAPAY

Target ORF information:

RefSeq Version	NM_123774.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana phytochelatin synthase 1 (PCS1) (CAD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_123774.4

ORF Insert Sequence:

ATGGCTATGG CGAGTTTATA TCGGCGATCT CTTCCTTCTC CTCCGGCCAT TGACTTTTCT 
TCCGCCGAAG GCAAGCTAAT CTTCAATGAA GCGCTTCAGA AAGGAACTAT GGAAGGATTT 
TTCAGGTTGA TTTCGTATTT TCAGACACAA TCCGAACCTG CGTATTGTGG TTTGGCTAGT 
CTCTCAGTGG TGTTGAATGC TCTTTCTATC GATCCTGGAC GTAAATGGAA AGGGCCTTGG 
AGGTGGTTTG ATGAATCAAT GTTGGATTGC TGCGAACCTC TGGAAGTAGT GAAGGAAAAA 
GGCATTTCAT TTGGAAAAGT TGTCTGTTTG GCTCATTGTT CAGGAGCAAA AGTTGAGGCT 
TTCCGTACAA GTCAGAGCAC CATTGATGAT TTCCGCAAAT TTGTCGTCAA ATGCACGAGT 
TCTGAGAATT GTCATATGAT CTCAACATAT CACCGAGGTG TATTTAAGCA GACTGGGACT 
GGTCACTTTT CACCTATTGG TGGCTATAAT GCTGAGAGAG ATATGGCTTT GATTCTTGAT 
GTTGCTCGTT TCAAGTATCC CCCTCACTGG GTTCCTCTTA AACTTCTTTG GGAAGCCATG 
GACAGTATTG ATCAGTCAAC AGGGAAACGT AGAGGGTTCA TGCTCATATC TAGACCACAC 
AGAGAACCCG GATTGCTCTA TACTCTGAGC TGCAAGGATG AAAGCTGGAT CGAAATAGCC 
AAGTATTTGA AGGAAGATGT TCCTCGTCTT GTAAGTTCAC AGCATGTAGA TTCTGTGGAG 
AAAATCATAT CAGTTGTGTT CAAGTCACTT CCATCAAATT TCAACCAATT CATCAGATGG 
GTGGCTGAGA TCCGAATTAC AGAGGACTCA AACCAAAATC TCAGCGCAGA GGAGAAGTCT 
AGGCTGAAAC TAAAGCAATT GGTGCTGAAG GAAGTGCACG AAACTGAACT GTTCAAACAC 
ATCAATAAGT TCTTATCCAC AGTGGGTTAT GAAGACAGTC TGACTTATGC TGCTGCAAAG 
GCTTGTTGCC AAGGAGCTGA AATCTTATCC GGAAGCCCAT CAAAAGAGTT TTGTTGTCGG 
GAAACTTGCG TGAAATGCAT CAAAGGTCCT GATGACTCTG AAGGCACGGT GGTGACTGGA 
GTTGTGGTGC GTGATGGGAA TGAACAAAAG GTTGATCTGT TAGTGCCATC GACGCAAACT 
GAGTGTGAAT GTGGTCCTGA AGCAACTTAT CCAGCAGGAA ACGATGTGTT CACTGCACTT 
CTATTGGCTT TACCTCCACA GACATGGTCA GGGATCAAAG ACCAAGCTCT TATGCATGAA 
ATGAAGCAGC TCATTTCCAT GGCTTCCCTC CCAACTTTGC TTCAAGAAGA GGTATTGCAT 
CTTCGACGGC AACTTCAGCT GCTAAAACGA TGCCAAGAGA ACAAGGAAGA GGATGATCTC 
GCTGCTCCTG CCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD1 ( NM_001344531.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001332549.1 Arabidopsis thaliana phytochelatin synthase 1 (PCS1) (CAD1), mRNA.

Price & Availability↑

CloneID	OAb41563
Clone ID Related Accession (Same CDS sequence)	NM_001344531.1
Accession Version	NM_001344531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	phytochelatin synthase 1 (PCS1)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGAAGGAT TTTTCAGGTT GATTTCGTAT TTTCAGACAC AATCCGAACC TGCGTATTGT 
GGTTTGGCTA GTCTCTCAGT GGTGTTGAAT GCTCTTTCTA TCGATCCTGG ACGTAAATGG 
AAAGGGCCTT GGAGGTGGTT TGATGAATCA ATGTTGGATT GCTGCGAACC TCTGGAAGTA 
GTGAAGGAAA AAGGCATTTC ATTTGGAAAA GTTGTCTGTT TGGCTCATTG TTCAGGAGCA 
AAAGTTGAGG CTTTCCGTAC AAGTCAGAGC ACCATTGATG ATTTCCGCAA ATTTGTCGTC 
AAATGCACGA GTTCTGAGAA TTGTCATATG ATCTCAACAT ATCACCGAGG TGTATTTAAG 
CAGACTGGGA CTGGTCACTT TTCACCTATT GGTGGCTATA ATGCTGAGAG AGATATGGCT 
TTGATTCTTG ATGTTGCTCG TTTCAAGTAT CCCCCTCACT GGGTTCCTCT TAAACTTCTT 
TGGGAAGCCA TGGACAGTAT TGATCAGTCA ACAGGGAAAC GTAGAGGGTT CATGCTCATA 
TCTAGACCAC ACAGAGAACC CGGATTGCTC TATACTCTGA GCTGCAAGGA TGAAAGCTGG 
ATCGAAATAG CCAAGTATTT GAAGGAAGAT GTTCCTCGTC TTGTAAGTTC ACAGCATGTA 
GATTCTGTGG AGAAAATCAT ATCAGTTGTG TTCAAGTCAC TTCCATCAAA TTTCAACCAA 
TTCATCAGAT GGGTGGCTGA GATCCGAATT ACAGAGGACT CAAACCAAAA TCTCAGCGCA 
GAGGAGAAGT CTAGGCTGAA ACTAAAGCAA TTGGTGCTGA AGGAAGTGCA CGAAACTGAA 
CTGTTCAAAC ACATCAATAA GTTCTTATCC ACAGTGGGTT ATGAAGACAG TCTGACTTAT 
GCTGCTGCAA AGGCTTGTTG CCAAGGAGCT GAAATCTTAT CCGGAAGCCC ATCAAAAGAG 
TTTTGTTGTC GGGAAACTTG CGTGAAATGC ATCAAAGGTC CTGATGACTC TGAAGGCACG 
GTGGTGACTG GAGTTGTGGT GCGTGATGGG AATGAACAAA AGGTTGATCT GTTAGTGCCA 
TCGACGCAAA CTGAGTGTGA ATGTGGTCCT GAAGCAACTT ATCCAGCAGG AAACGATGTG 
TTCACTGCAC TTCTATTGGC TTTACCTCCA CAGACATGGT CAGGGATCAA AGACCAAGCT 
CTTATGCATG AAATGAAGCA GCTCATTTCC ATGGCTTCCC TCCCAACTTT GCTTCAAGAA 
GAGGTATTGC ATCTTCGACG GCAACTTCAG CTGCTAAAAC GATGCCAAGA GAACAAGGAA 
GAGGATGATC TCGCTGCTCC TGCCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001332549.1
CDS	549..1898
Translation	MEGFFRLISYFQTQSEPAYCGLASLSVVLNALSIDPGRKWKGPWRWFDESMLDCCEPLEVVKEKGISFGKVVCLAHCSGAKVEAFRTSQSTIDDFRKFVVKCTSSENCHMISTYHRGVFKQTGTGHFSPIGGYNAERDMALILDVARFKYPPHWVPLKLLWEAMDSIDQSTGKRRGFMLISRPHREPGLLYTLSCKDESWIEIAKYLKEDVPRLVSSQHVDSVEKIISVVFKSLPSNFNQFIRWVAEIRITEDSNQNLSAEEKSRLKLKQLVLKEVHETELFKHINKFLSTVGYEDSLTYAAAKACCQGAEILSGSPSKEFCCRETCVKCIKGPDDSEGTVVTGVVVRDGNEQKVDLLVPSTQTECECGPEATYPAGNDVFTALLLALPPQTWSGIKDQALMHEMKQLISMASLPTLLQEEVLHLRRQLQLLKRCQENKEEDDLAAPAY

Target ORF information:

RefSeq Version	NM_001344531.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana phytochelatin synthase 1 (PCS1) (CAD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001344531.1

ORF Insert Sequence:

ATGGAAGGAT TTTTCAGGTT GATTTCGTAT TTTCAGACAC AATCCGAACC TGCGTATTGT 
GGTTTGGCTA GTCTCTCAGT GGTGTTGAAT GCTCTTTCTA TCGATCCTGG ACGTAAATGG 
AAAGGGCCTT GGAGGTGGTT TGATGAATCA ATGTTGGATT GCTGCGAACC TCTGGAAGTA 
GTGAAGGAAA AAGGCATTTC ATTTGGAAAA GTTGTCTGTT TGGCTCATTG TTCAGGAGCA 
AAAGTTGAGG CTTTCCGTAC AAGTCAGAGC ACCATTGATG ATTTCCGCAA ATTTGTCGTC 
AAATGCACGA GTTCTGAGAA TTGTCATATG ATCTCAACAT ATCACCGAGG TGTATTTAAG 
CAGACTGGGA CTGGTCACTT TTCACCTATT GGTGGCTATA ATGCTGAGAG AGATATGGCT 
TTGATTCTTG ATGTTGCTCG TTTCAAGTAT CCCCCTCACT GGGTTCCTCT TAAACTTCTT 
TGGGAAGCCA TGGACAGTAT TGATCAGTCA ACAGGGAAAC GTAGAGGGTT CATGCTCATA 
TCTAGACCAC ACAGAGAACC CGGATTGCTC TATACTCTGA GCTGCAAGGA TGAAAGCTGG 
ATCGAAATAG CCAAGTATTT GAAGGAAGAT GTTCCTCGTC TTGTAAGTTC ACAGCATGTA 
GATTCTGTGG AGAAAATCAT ATCAGTTGTG TTCAAGTCAC TTCCATCAAA TTTCAACCAA 
TTCATCAGAT GGGTGGCTGA GATCCGAATT ACAGAGGACT CAAACCAAAA TCTCAGCGCA 
GAGGAGAAGT CTAGGCTGAA ACTAAAGCAA TTGGTGCTGA AGGAAGTGCA CGAAACTGAA 
CTGTTCAAAC ACATCAATAA GTTCTTATCC ACAGTGGGTT ATGAAGACAG TCTGACTTAT 
GCTGCTGCAA AGGCTTGTTG CCAAGGAGCT GAAATCTTAT CCGGAAGCCC ATCAAAAGAG 
TTTTGTTGTC GGGAAACTTG CGTGAAATGC ATCAAAGGTC CTGATGACTC TGAAGGCACG 
GTGGTGACTG GAGTTGTGGT GCGTGATGGG AATGAACAAA AGGTTGATCT GTTAGTGCCA 
TCGACGCAAA CTGAGTGTGA ATGTGGTCCT GAAGCAACTT ATCCAGCAGG AAACGATGTG 
TTCACTGCAC TTCTATTGGC TTTACCTCCA CAGACATGGT CAGGGATCAA AGACCAAGCT 
CTTATGCATG AAATGAAGCA GCTCATTTCC ATGGCTTCCC TCCCAACTTT GCTTCAAGAA 
GAGGTATTGC ATCTTCGACG GCAACTTCAG CTGCTAAAAC GATGCCAAGA GAACAAGGAA 
GAGGATGATC TCGCTGCTCC TGCCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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CAD1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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