SPP cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPP
Entrez Gene ID	834245
Full Name	Insulinase (Peptidase family M16) family protein
Synonyms	MDH9.8,MDH9_8,stromal processing peptidase
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001344443.1	NP_001331624.1	Insulinase (Peptidase family M16) family protein
NM_123604.3	NP_199054.1	Insulinase (Peptidase family M16) family protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT5G42390	NP_199054.1
Oryza sativa (rice)	Os06g0625400	NP_001058112.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that one copy of the SPP gene (At5g42390) is able to produce sufficient SPP protein for normal development under standard growth conditions.
Title: The stromal processing peptidase of chloroplasts is essential in Arabidopsis, with knockout mutations causing embryo arrest after the 16-cell stage.

The following SPP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb41516	NM_001344443.1 Latest version!	Arabidopsis thaliana Insulinase (Peptidase family M16) family protein (SPP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAb20328	NM_123604.3 Latest version!	Arabidopsis thaliana Insulinase (Peptidase family M16) family protein (SPP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPP ( NM_001344443.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001331624.1 Arabidopsis thaliana Insulinase (Peptidase family M16) family protein (SPP), mRNA.

Price & Availability↑

CloneID	OAb41516
Clone ID Related Accession (Same CDS sequence)	NM_001344443.1
Accession Version	NM_001344443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Insulinase (Peptidase family M16) family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGCTTCAT CGTCCTCTTC CATTTTCACC GGTGTTAAGT TCTCTCCGAT CTTAGCTCCC 
TTTAACTCCG GAGATAGCCG CCGCTCTCGA TATCTAAAAG ATAGCCGGAA TAAAGTTAGG 
TTTAATCCAT CGTCGCCGCG TCTCACTCCT CATCGTGTTC GCGTCGAAGC TCCGTCTTTA 
ATTCCCTATA ATGGTCTTTG GGCGGCGCAG CCAAATTCGC ATAAGGGACG TTTAAAGAGG 
AACATTGTTT CGGGAAAAGA AGCTACCGGG ATATCACTTT CTCAAGGACG TAATTTCTGT 
CTTACTTGTA AAAGGAATCA AGCTGGAATC AGAAGAGCAT TACCTAGTGC CTTTGTGGAT 
AGAACTGCAT TTAGTTTGTC GAGGTCGAGT TTGACATCTT CTCTGAGGAA ACATTCTCAG 
ATTGTGAATG CAACTCTTGG ACCAGATGAG CCACATGCTG CTGGTACAGC TTGGCCTGAT 
GGTATTGTTG CGGAGAGACA AGATCTCGAC TTATTGCCTC CTGAGATTGA TAGTGCAGAG 
CTAGAAGCGT TTCTTGGTTG TGAACTTCCT TCTCATCCAA AGTTGCACCG GGGTCAATTG 
AAAAATGGGC TTCGATATCT TATTTTGCCA AACAAAGTTC CACCGAACAG ATTTGAGGCA 
CACATGGAAG TTCATGTAGG ATCGATTGAT GAGGAAGAAG ATGAGCAAGG GATTGCTCAT 
ATGATAGAAC ATGTTGCTTT CCTTGGGAGC AAGAAACGTG AGAAACTTCT TGGTACAGGT 
GCCCGCTCTA ATGCCTACAC CGATTTCCAC CATACAGTAT TTCATATTCA TTCTCCAACC 
CACACGAAGG ACTCTGAGGA TGACCTCTTC CCATCTGTGC TCGATGCCTT AAATGAGATA 
GCCTTTCACC CAAAATTTCT CTCTTCTCGA GTTGAGAAAG AAAGGCGGGC CATACTTTCA 
GAACTTCAGA TGATGAATAC AATTGAGTAT CGTGTTGACT GCCAGTTGTT GCAACATCTA 
CATTCTGAGA ACAAGCTGGG TAGGAGGTTC CCTATTGGAT TGGAAGAGCA GATTAAGAAG 
TGGGATGTTG ACAAAATTAG AAAATTTCAT GAGCGATGGT ACTTCCCAGC CAATGCCACT 
CTATATATTG TGGGAGATAT TGACAACATA CCTCGGATAG TCCACAATAT TGAAGCTGTG 
TTTGGAAAAA ATGGTTTGGA TAATGAATCA ACACCCAGTT CTCCTAGTCC TGGTGCCTTC 
GGTGCCATGG CTAATTTTCT CGTTCCAAAG CTGCCAGCTG GTCTTGGTGG TACCTTTTCA 
AATGAGAAAA CAAATACTGC TGATCAGTCT AAGATGATTA AAAGAGAAAG ACATGCAATT 
CGTCCCCCGG TTGAGCATAA TTGGTCGCTT CCTGGAACAA GTGTTGATCT AAAGCCTCCA 
CAGATATTTA AGCACGAGCT TCTTCAGAAT TTTGCAATCA ACATGTTTTG CAAGATACCT 
GTTAGCAAGG TTCAGACATT TGGAGACCTG CGAAATGTTC TGATGAAAAG AATATTTCTC 
TCTGCTCTAC ATTTTCGAAT TAATACGAGA TACAAGAGTT CAAACCCACC ATTTACGTCT 
GTTGAACTGG ACCATAGTGA TTCGGGAAGG GAAGGATGCA CTGTTACGAC TCTTACTGTC 
ACCGCAGAGC CCCAAAATTG GCAAAATGCA GTCAAAGTTG CTGTCCAAGA GGTTCGAAGA 
CTTAAAGAAT TTGGTGTCAC CAGGGGTGAA CTTACACGTT ACATGGATGC TCTTCTAAAA 
GATAGTGAAC ATCTTGCAGC TATGATAGAT AATGTGTCTT CTGTTGATAA CCTGGATTTT 
ATCATGGAAA GTGATGCACT AAGCCACACG GTTATGGATC AGACGCAAGG ACATGAGACC 
TTGGTTGCTG TAGCTGGAAC TGTTACTCTT GAAGAGGTAA ACACTGTTGG CGCCAAGGTG 
CTAGAATTTA TTTCTGATTT CGGAAGACCC ACTGCCCCTC TTCCCGCAGC AATAGTTGCT 
TGTGTTCCCA CTAAGGTGCA TGTTGATGGG GTTGGTGAGA GTGATTTCAA TATATCTCCT 
GATGAAATAA TAGAGTCTGT CAAATCAGGA TTATTAGCAC CCATTGAAGC TGAGCCTGAG 
CTCGAGGTGC CAAAAGAATT GATCTCTCAA TCACAATTGA AAGAGTTAAC CTTGCAGAGA 
AATCCATGTT TTGTCCCTAT TCCTGGGTCA GGTTTAACCA AATTGCATGA CAAAGAGACT 
GGGATCACTC AGCTTCGGTT ATCCAATGGG ATAGCTGTAA ATTACAAGAA ATCTACGACC 
GAATCCCGGG CAGGTGTCAT GCGGCTCATT GTTGGTGGTG GACGAGCTGC TGAAACATCT 
GATTCCAAAG GAGCTGTGGT TGTTGGTGTT CGTACACTCA GCGAGGGTGG TCGAGTTGGA 
GACTTTTCAA GGGAGCAGGT GGAGCTGTTC TGTGTAAATC ATTTGATAAA CTGTTCGTTG 
GAGTCAACTG AGGAGTTCAT TGCCATGGAA TTCCGGTTTA CTCTGAGAGA CAATGGAATG 
CAAGCGGCTT TTCAGCTGCT TCACATGGTG CTTGAGCGTA GTGTCTGGTT GGAAGATGCA 
TTTGATCGAG CAAGGCAGCT ATATCTTTCG TATTTTCGAT CAATTCCTAA AAGCTTGGAG 
CGTGCGACTG CTCATAAACT TATGATCGCT ATGCTGAACG GTGATGAGCG TTTTGTTGAG 
CCGACACCTA AGTCACTACA GAGCTTGAAT CTAGAATCTG TGAAAGATGC GGTCATGAGT 
CATTTTGTTG GGGACAATAT GGAGGTAAGC ATTGTGGGGG ACTTTTCAGA GGAGGAAATC 
GAGCGTTGCA TCCTTGATTA CCTTGGTACA GTTAAAGCTA GCCATGATTC TGCAAAACCA 
CCAGGATCTG AGCCTATCTT GTTTCGCCAA CCTACAGCTG GCTTGCAATT TCAACAGGTA 
TTCTTGAAGG ATACTGATGA GAGAGCATGT GCATACATTG CGGGACCAGC GCCAAATCGA 
TGGGGCTTTA CAGTTGACGG GGATGACCTT TTTCAGTCTG TTAGCAAACT TCCTGTTGCT 
CATGATGGAC TTTTGAAATC GGAGGAACAA TTGCTAGAGG GAGGAGACAG GGAGTTGCAA 
AAGAAGCTTC GTGCCCACCC TCTTTTCTTT GGAGTTACAA TGGGTCTTCT GGCCGAAATC 
ATAAACTCAA GAATTTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001331624.1
CDS	141..3401
Translation	MASSSSSIFTGVKFSPILAPFNSGDSRRSRYLKDSRNKVRFNPSSPRLTPHRVRVEAPSLIPYNGLWAAQPNSHKGRLKRNIVSGKEATGISLSQGRNFCLTCKRNQAGIRRALPSAFVDRTAFSLSRSSLTSSLRKHSQIVNATLGPDEPHAAGTAWPDGIVAERQDLDLLPPEIDSAELEAFLGCELPSHPKLHRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEEDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTHTKDSEDDLFPSVLDALNEIAFHPKFLSSRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLGRRFPIGLEEQIKKWDVDKIRKFHERWYFPANATLYIVGDIDNIPRIVHNIEAVFGKNGLDNESTPSSPSPGAFGAMANFLVPKLPAGLGGTFSNEKTNTADQSKMIKRERHAIRPPVEHNWSLPGTSVDLKPPQIFKHELLQNFAINMFCKIPVSKVQTFGDLRNVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGREGCTVTTLTVTAEPQNWQNAVKVAVQEVRRLKEFGVTRGELTRYMDALLKDSEHLAAMIDNVSSVDNLDFIMESDALSHTVMDQTQGHETLVAVAGTVTLEEVNTVGAKVLEFISDFGRPTAPLPAAIVACVPTKVHVDGVGESDFNISPDEIIESVKSGLLAPIEAEPELEVPKELISQSQLKELTLQRNPCFVPIPGSGLTKLHDKETGITQLRLSNGIAVNYKKSTTESRAGVMRLIVGGGRAAETSDSKGAVVVGVRTLSEGGRVGDFSREQVELFCVNHLINCSLESTEEFIAMEFRFTLRDNGMQAAFQLLHMVLERSVWLEDAFDRARQLYLSYFRSIPKSLERATAHKLMIAMLNGDERFVEPTPKSLQSLNLESVKDAVMSHFVGDNMEVSIVGDFSEEEIERCILDYLGTVKASHDSAKPPGSEPILFRQPTAGLQFQQVFLKDTDERACAYIAGPAPNRWGFTVDGDDLFQSVSKLPVAHDGLLKSEEQLLEGGDRELQKKLRAHPLFFGVTMGLLAEIINSRIC

Target ORF information:

RefSeq Version	NM_001344443.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Insulinase (Peptidase family M16) family protein (SPP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001344443.1

ORF Insert Sequence:

ATGGCTTCAT CGTCCTCTTC CATTTTCACC GGTGTTAAGT TCTCTCCGAT CTTAGCTCCC 
TTTAACTCCG GAGATAGCCG CCGCTCTCGA TATCTAAAAG ATAGCCGGAA TAAAGTTAGG 
TTTAATCCAT CGTCGCCGCG TCTCACTCCT CATCGTGTTC GCGTCGAAGC TCCGTCTTTA 
ATTCCCTATA ATGGTCTTTG GGCGGCGCAG CCAAATTCGC ATAAGGGACG TTTAAAGAGG 
AACATTGTTT CGGGAAAAGA AGCTACCGGG ATATCACTTT CTCAAGGACG TAATTTCTGT 
CTTACTTGTA AAAGGAATCA AGCTGGAATC AGAAGAGCAT TACCTAGTGC CTTTGTGGAT 
AGAACTGCAT TTAGTTTGTC GAGGTCGAGT TTGACATCTT CTCTGAGGAA ACATTCTCAG 
ATTGTGAATG CAACTCTTGG ACCAGATGAG CCACATGCTG CTGGTACAGC TTGGCCTGAT 
GGTATTGTTG CGGAGAGACA AGATCTCGAC TTATTGCCTC CTGAGATTGA TAGTGCAGAG 
CTAGAAGCGT TTCTTGGTTG TGAACTTCCT TCTCATCCAA AGTTGCACCG GGGTCAATTG 
AAAAATGGGC TTCGATATCT TATTTTGCCA AACAAAGTTC CACCGAACAG ATTTGAGGCA 
CACATGGAAG TTCATGTAGG ATCGATTGAT GAGGAAGAAG ATGAGCAAGG GATTGCTCAT 
ATGATAGAAC ATGTTGCTTT CCTTGGGAGC AAGAAACGTG AGAAACTTCT TGGTACAGGT 
GCCCGCTCTA ATGCCTACAC CGATTTCCAC CATACAGTAT TTCATATTCA TTCTCCAACC 
CACACGAAGG ACTCTGAGGA TGACCTCTTC CCATCTGTGC TCGATGCCTT AAATGAGATA 
GCCTTTCACC CAAAATTTCT CTCTTCTCGA GTTGAGAAAG AAAGGCGGGC CATACTTTCA 
GAACTTCAGA TGATGAATAC AATTGAGTAT CGTGTTGACT GCCAGTTGTT GCAACATCTA 
CATTCTGAGA ACAAGCTGGG TAGGAGGTTC CCTATTGGAT TGGAAGAGCA GATTAAGAAG 
TGGGATGTTG ACAAAATTAG AAAATTTCAT GAGCGATGGT ACTTCCCAGC CAATGCCACT 
CTATATATTG TGGGAGATAT TGACAACATA CCTCGGATAG TCCACAATAT TGAAGCTGTG 
TTTGGAAAAA ATGGTTTGGA TAATGAATCA ACACCCAGTT CTCCTAGTCC TGGTGCCTTC 
GGTGCCATGG CTAATTTTCT CGTTCCAAAG CTGCCAGCTG GTCTTGGTGG TACCTTTTCA 
AATGAGAAAA CAAATACTGC TGATCAGTCT AAGATGATTA AAAGAGAAAG ACATGCAATT 
CGTCCCCCGG TTGAGCATAA TTGGTCGCTT CCTGGAACAA GTGTTGATCT AAAGCCTCCA 
CAGATATTTA AGCACGAGCT TCTTCAGAAT TTTGCAATCA ACATGTTTTG CAAGATACCT 
GTTAGCAAGG TTCAGACATT TGGAGACCTG CGAAATGTTC TGATGAAAAG AATATTTCTC 
TCTGCTCTAC ATTTTCGAAT TAATACGAGA TACAAGAGTT CAAACCCACC ATTTACGTCT 
GTTGAACTGG ACCATAGTGA TTCGGGAAGG GAAGGATGCA CTGTTACGAC TCTTACTGTC 
ACCGCAGAGC CCCAAAATTG GCAAAATGCA GTCAAAGTTG CTGTCCAAGA GGTTCGAAGA 
CTTAAAGAAT TTGGTGTCAC CAGGGGTGAA CTTACACGTT ACATGGATGC TCTTCTAAAA 
GATAGTGAAC ATCTTGCAGC TATGATAGAT AATGTGTCTT CTGTTGATAA CCTGGATTTT 
ATCATGGAAA GTGATGCACT AAGCCACACG GTTATGGATC AGACGCAAGG ACATGAGACC 
TTGGTTGCTG TAGCTGGAAC TGTTACTCTT GAAGAGGTAA ACACTGTTGG CGCCAAGGTG 
CTAGAATTTA TTTCTGATTT CGGAAGACCC ACTGCCCCTC TTCCCGCAGC AATAGTTGCT 
TGTGTTCCCA CTAAGGTGCA TGTTGATGGG GTTGGTGAGA GTGATTTCAA TATATCTCCT 
GATGAAATAA TAGAGTCTGT CAAATCAGGA TTATTAGCAC CCATTGAAGC TGAGCCTGAG 
CTCGAGGTGC CAAAAGAATT GATCTCTCAA TCACAATTGA AAGAGTTAAC CTTGCAGAGA 
AATCCATGTT TTGTCCCTAT TCCTGGGTCA GGTTTAACCA AATTGCATGA CAAAGAGACT 
GGGATCACTC AGCTTCGGTT ATCCAATGGG ATAGCTGTAA ATTACAAGAA ATCTACGACC 
GAATCCCGGG CAGGTGTCAT GCGGCTCATT GTTGGTGGTG GACGAGCTGC TGAAACATCT 
GATTCCAAAG GAGCTGTGGT TGTTGGTGTT CGTACACTCA GCGAGGGTGG TCGAGTTGGA 
GACTTTTCAA GGGAGCAGGT GGAGCTGTTC TGTGTAAATC ATTTGATAAA CTGTTCGTTG 
GAGTCAACTG AGGAGTTCAT TGCCATGGAA TTCCGGTTTA CTCTGAGAGA CAATGGAATG 
CAAGCGGCTT TTCAGCTGCT TCACATGGTG CTTGAGCGTA GTGTCTGGTT GGAAGATGCA 
TTTGATCGAG CAAGGCAGCT ATATCTTTCG TATTTTCGAT CAATTCCTAA AAGCTTGGAG 
CGTGCGACTG CTCATAAACT TATGATCGCT ATGCTGAACG GTGATGAGCG TTTTGTTGAG 
CCGACACCTA AGTCACTACA GAGCTTGAAT CTAGAATCTG TGAAAGATGC GGTCATGAGT 
CATTTTGTTG GGGACAATAT GGAGGTAAGC ATTGTGGGGG ACTTTTCAGA GGAGGAAATC 
GAGCGTTGCA TCCTTGATTA CCTTGGTACA GTTAAAGCTA GCCATGATTC TGCAAAACCA 
CCAGGATCTG AGCCTATCTT GTTTCGCCAA CCTACAGCTG GCTTGCAATT TCAACAGGTA 
TTCTTGAAGG ATACTGATGA GAGAGCATGT GCATACATTG CGGGACCAGC GCCAAATCGA 
TGGGGCTTTA CAGTTGACGG GGATGACCTT TTTCAGTCTG TTAGCAAACT TCCTGTTGCT 
CATGATGGAC TTTTGAAATC GGAGGAACAA TTGCTAGAGG GAGGAGACAG GGAGTTGCAA 
AAGAAGCTTC GTGCCCACCC TCTTTTCTTT GGAGTTACAA TGGGTCTTCT GGCCGAAATC 
ATAAACTCAA GAATTTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPP ( NM_123604.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_199054.1 Arabidopsis thaliana Insulinase (Peptidase family M16) family protein (SPP), mRNA.

Price & Availability↑

CloneID	OAb20328
Clone ID Related Accession (Same CDS sequence)	NM_123604.3
Accession Version	NM_123604.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Insulinase (Peptidase family M16) family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_123604.2.

ATGGCTTCAT CGTCCTCTTC CATTTTCACC GGTGTTAAGT TCTCTCCGAT CTTAGCTCCC 
TTTAACTCCG GAGATAGCCG CCGCTCTCGA TATCTAAAAG ATAGCCGGAA TAAAGTTAGG 
TTTAATCCAT CGTCGCCGCG TCTCACTCCT CATCGTGTTC GCGTCGAAGC TCCGTCTTTA 
ATTCCCTATA ATGGTCTTTG GGCGGCGCAG CCAAATTCGC ATAAGGGACG TTTAAAGAGG 
AACATTGTTT CGGGAAAAGA AGCTACCGGG ATATCACTTT CTCAAGGACG TAATTTCTGT 
CTTACTTGTA AAAGGAATCA AGCTGGAATC AGAAGAGCAT TACCTAGTGC CTTTGTGGAT 
AGAACTGCAT TTAGTTTGTC GAGGTCGAGT TTGACATCTT CTCTGAGGAA ACATTCTCAG 
ATTGTGAATG CAACTCTTGG ACCAGATGAG CCACATGCTG CTGGTACAGC TTGGCCTGAT 
GGTATTGTTG CGGAGAGACA AGATCTCGAC TTATTGCCTC CTGAGATTGA TAGTGCAGAG 
CTAGAAGCGT TTCTTGGTTG TGAACTTCCT TCTCATCCAA AGTTGCACCG GGGTCAATTG 
AAAAATGGGC TTCGATATCT TATTTTGCCA AACAAAGTTC CACCGAACAG ATTTGAGGCA 
CACATGGAAG TTCATGTAGG ATCGATTGAT GAGGAAGAAG ATGAGCAAGG GATTGCTCAT 
ATGATAGAAC ATGTTGCTTT CCTTGGGAGC AAGAAACGTG AGAAACTTCT TGGTACAGGT 
GCCCGCTCTA ATGCCTACAC CGATTTCCAC CATACAGTAT TTCATATTCA TTCTCCAACC 
CACACGAAGG ACTCTGAGGA TGACCTCTTC CCATCTGTGC TCGATGCCTT AAATGAGATA 
GCCTTTCACC CAAAATTTCT CTCTTCTCGA GTTGAGAAAG AAAGGCGGGC CATACTTTCA 
GAACTTCAGA TGATGAATAC AATTGAGTAT CGTGTTGACT GCCAGTTGTT GCAACATCTA 
CATTCTGAGA ACAAGCTGGG TAGGAGGTTC CCTATTGGAT TGGAAGAGCA GATTAAGAAG 
TGGGATGTTG ACAAAATTAG AAAATTTCAT GAGCGATGGT ACTTCCCAGC CAATGCCACT 
CTATATATTG TGGGAGATAT TGACAACATA CCTCGGATAG TCCACAATAT TGAAGCTGTG 
TTTGGAAAAA ATGGTTTGGA TAATGAATCA ACACCCAGTT CTCCTAGTCC TGGTGCCTTC 
GGTGCCATGG CTAATTTTCT CGTTCCAAAG CTGCCAGCTG GTCTTGGTGG TACCTTTTCA 
AATGAGAAAA CAAATACTGC TGATCAGTCT AAGATGATTA AAAGAGAAAG ACATGCAATT 
CGTCCCCCGG TTGAGCATAA TTGGTCGCTT CCTGGAACAA GTGTTGATCT AAAGCCTCCA 
CAGATATTTA AGCACGAGCT TCTTCAGAAT TTTGCAATCA ACATGTTTTG CAAGATACCT 
GTTAGCAAGG TTCAGACATT TGGAGACCTG CGAAATGTTC TGATGAAAAG AATATTTCTC 
TCTGCTCTAC ATTTTCGAAT TAATACGAGA TACAAGAGTT CAAACCCACC ATTTACGTCT 
GTTGAACTGG ACCATAGTGA TTCGGGAAGG GAAGGATGCA CTGTTACGAC TCTTACTGTC 
ACCGCAGAGC CCCAAAATTG GCAAAATGCA GTCAAAGTTG CTGTCCAAGA GGTTCGAAGA 
CTTAAAGAAT TTGGTGTCAC CAGGGGTGAA CTTACACGTT ACATGGATGC TCTTCTAAAA 
GATAGTGAAC ATCTTGCAGC TATGATAGAT AATGTGTCTT CTGTTGATAA CCTGGATTTT 
ATCATGGAAA GTGATGCACT AAGCCACACG GTTATGGATC AGACGCAAGG ACATGAGACC 
TTGGTTGCTG TAGCTGGAAC TGTTACTCTT GAAGAGGTAA ACACTGTTGG CGCCAAGGTG 
CTAGAATTTA TTTCTGATTT CGGAAGACCC ACTGCCCCTC TTCCCGCAGC AATAGTTGCT 
TGTGTTCCCA CTAAGGTGCA TGTTGATGGG GTTGGTGAGA GTGATTTCAA TATATCTCCT 
GATGAAATAA TAGAGTCTGT CAAATCAGGA TTATTAGCAC CCATTGAAGC TGAGCCTGAG 
CTCGAGGTGC CAAAAGAATT GATCTCTCAA TCACAATTGA AAGAGTTAAC CTTGCAGAGA 
AATCCATGTT TTGTCCCTAT TCCTGGGTCA GGTTTAACCA AATTGCATGA CAAAGAGACT 
GGGATCACTC AGCTTCGGTT ATCCAATGGG ATAGCTGTAA ATTACAAGAA ATCTACGACC 
GAATCCCGGG CAGGTGTCAT GCGGCTCATT GTTGGTGGTG GACGAGCTGC TGAAACATCT 
GATTCCAAAG GAGCTGTGGT TGTTGGTGTT CGTACACTCA GCGAGGGTGG TCGAGTTGGA 
GACTTTTCAA GGGAGCAGGT GGAGCTGTTC TGTGTAAATC ATTTGATAAA CTGTTCGTTG 
GAGTCAACTG AGGAGTTCAT TGCCATGGAA TTCCGGTTTA CTCTGAGAGA CAATGGAATG 
CAAGCGGCTT TTCAGCTGCT TCACATGGTG CTTGAGCGTA GTGTCTGGTT GGAAGATGCA 
TTTGATCGAG CAAGGCAGCT ATATCTTTCG TATTTTCGAT CAATTCCTAA AAGCTTGGAG 
CGTGCGACTG CTCATAAACT TATGATCGCT ATGCTGAACG GTGATGAGCG TTTTGTTGAG 
CCGACACCTA AGTCACTACA GAGCTTGAAT CTAGAATCTG TGAAAGATGC GGTCATGAGT 
CATTTTGTTG GGGACAATAT GGAGGTAAGC ATTGTGGGGG ACTTTTCAGA GGAGGAAATC 
GAGCGTTGCA TCCTTGATTA CCTTGGTACA GTTAAAGCTA GCCATGATTC TGCAAAACCA 
CCAGGATCTG AGCCTATCTT GTTTCGCCAA CCTACAGCTG GCTTGCAATT TCAACAGGTA 
TTCTTGAAGG ATACTGATGA GAGAGCATGT GCATACATTG CGGGACCAGC GCCAAATCGA 
TGGGGCTTTA CAGTTGACGG GGATGACCTT TTTCAGTCTG TTAGCAAACT TCCTGTTGCT 
CATGATGGAC TTTTGAAATC GGAGGAACAA TTGCTAGAGG GAGGAGACAG GGAGTTGCAA 
AAGAAGCTTC GTGCCCACCC TCTTTTCTTT GGAGTTACAA TGGGTCTTCT GGCCGAAATC 
ATAAACTCAA GGCTTTTCAC GACAGTAAGA GATTCTCTTG GACTGACATA TGACGTATCA 
TTTGAGCTAA ACCTATTTGA TAGGCTGAAT CTCGGGTGGT ATGTGATATC TGTGACTTCA 
ACTCCCGGCA AGGTATACAA AGCGGTTGAT GCCTGTAAGA GTGTTCTTAG AGGTTTGCAT 
AGCAACCAAA TTGCCCCTAG GGAGTTGGAT AGGGCAAAGC GAACTCTTCT AATGAGACAT 
GAAGCTGAAC TCAAGTCCAA TGCCTATTGG CTTAATCTAT TAGCTCACTT GCAGGCCTCC 
TCTGTTCCGA GGAAGGAACT GTCATGCATT AAAGAACTTG TTTCATTGTA TGAAGCTGCT 
TCGATCGAGG ACATATACCT AGCTTATAAT CAATTAAGAG TGGACGAGGA CTCTCTGTAC 
TCCTGTATTG GTATTGCTGG GGCACAGGCG GGTGAAGAGA TTACTGTTCT CTCAGAAGAG 
GAAGAACCAG AAGATGTTTT CTCAGGAGTT GTTCCTGTGG GACGAGGCTC ATCGATGACC 
ACAAGACCAA CAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_199054.1
CDS	144..3941
Translation	MASSSSSIFTGVKFSPILAPFNSGDSRRSRYLKDSRNKVRFNPSSPRLTPHRVRVEAPSLIPYNGLWAAQPNSHKGRLKRNIVSGKEATGISLSQGRNFCLTCKRNQAGIRRALPSAFVDRTAFSLSRSSLTSSLRKHSQIVNATLGPDEPHAAGTAWPDGIVAERQDLDLLPPEIDSAELEAFLGCELPSHPKLHRGQLKNGLRYLILPNKVPPNRFEAHMEVHVGSIDEEEDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTHTKDSEDDLFPSVLDALNEIAFHPKFLSSRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLGRRFPIGLEEQIKKWDVDKIRKFHERWYFPANATLYIVGDIDNIPRIVHNIEAVFGKNGLDNESTPSSPSPGAFGAMANFLVPKLPAGLGGTFSNEKTNTADQSKMIKRERHAIRPPVEHNWSLPGTSVDLKPPQIFKHELLQNFAINMFCKIPVSKVQTFGDLRNVLMKRIFLSALHFRINTRYKSSNPPFTSVELDHSDSGREGCTVTTLTVTAEPQNWQNAVKVAVQEVRRLKEFGVTRGELTRYMDALLKDSEHLAAMIDNVSSVDNLDFIMESDALSHTVMDQTQGHETLVAVAGTVTLEEVNTVGAKVLEFISDFGRPTAPLPAAIVACVPTKVHVDGVGESDFNISPDEIIESVKSGLLAPIEAEPELEVPKELISQSQLKELTLQRNPCFVPIPGSGLTKLHDKETGITQLRLSNGIAVNYKKSTTESRAGVMRLIVGGGRAAETSDSKGAVVVGVRTLSEGGRVGDFSREQVELFCVNHLINCSLESTEEFIAMEFRFTLRDNGMQAAFQLLHMVLERSVWLEDAFDRARQLYLSYFRSIPKSLERATAHKLMIAMLNGDERFVEPTPKSLQSLNLESVKDAVMSHFVGDNMEVSIVGDFSEEEIERCILDYLGTVKASHDSAKPPGSEPILFRQPTAGLQFQQVFLKDTDERACAYIAGPAPNRWGFTVDGDDLFQSVSKLPVAHDGLLKSEEQLLEGGDRELQKKLRAHPLFFGVTMGLLAEIINSRLFTTVRDSLGLTYDVSFELNLFDRLNLGWYVISVTSTPGKVYKAVDACKSVLRGLHSNQIAPRELDRAKRTLLMRHEAELKSNAYWLNLLAHLQASSVPRKELSCIKELVSLYEAASIEDIYLAYNQLRVDEDSLYSCIGIAGAQAGEEITVLSEEEEPEDVFSGVVPVGRGSSMTTRPTT

Target ORF information:

RefSeq Version	NM_123604.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Insulinase (Peptidase family M16) family protein (SPP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_123604.3

ORF Insert Sequence:

ATGGCTTCAT CGTCCTCTTC CATTTTCACC GGTGTTAAGT TCTCTCCGAT CTTAGCTCCC 
TTTAACTCCG GAGATAGCCG CCGCTCTCGA TATCTAAAAG ATAGCCGGAA TAAAGTTAGG 
TTTAATCCAT CGTCGCCGCG TCTCACTCCT CATCGTGTTC GCGTCGAAGC TCCGTCTTTA 
ATTCCCTATA ATGGTCTTTG GGCGGCGCAG CCAAATTCGC ATAAGGGACG TTTAAAGAGG 
AACATTGTTT CGGGAAAAGA AGCTACCGGG ATATCACTTT CTCAAGGACG TAATTTCTGT 
CTTACTTGTA AAAGGAATCA AGCTGGAATC AGAAGAGCAT TACCTAGTGC CTTTGTGGAT 
AGAACTGCAT TTAGTTTGTC GAGGTCGAGT TTGACATCTT CTCTGAGGAA ACATTCTCAG 
ATTGTGAATG CAACTCTTGG ACCAGATGAG CCACATGCTG CTGGTACAGC TTGGCCTGAT 
GGTATTGTTG CGGAGAGACA AGATCTCGAC TTATTGCCTC CTGAGATTGA TAGTGCAGAG 
CTAGAAGCGT TTCTTGGTTG TGAACTTCCT TCTCATCCAA AGTTGCACCG GGGTCAATTG 
AAAAATGGGC TTCGATATCT TATTTTGCCA AACAAAGTTC CACCGAACAG ATTTGAGGCA 
CACATGGAAG TTCATGTAGG ATCGATTGAT GAGGAAGAAG ATGAGCAAGG GATTGCTCAT 
ATGATAGAAC ATGTTGCTTT CCTTGGGAGC AAGAAACGTG AGAAACTTCT TGGTACAGGT 
GCCCGCTCTA ATGCCTACAC CGATTTCCAC CATACAGTAT TTCATATTCA TTCTCCAACC 
CACACGAAGG ACTCTGAGGA TGACCTCTTC CCATCTGTGC TCGATGCCTT AAATGAGATA 
GCCTTTCACC CAAAATTTCT CTCTTCTCGA GTTGAGAAAG AAAGGCGGGC CATACTTTCA 
GAACTTCAGA TGATGAATAC AATTGAGTAT CGTGTTGACT GCCAGTTGTT GCAACATCTA 
CATTCTGAGA ACAAGCTGGG TAGGAGGTTC CCTATTGGAT TGGAAGAGCA GATTAAGAAG 
TGGGATGTTG ACAAAATTAG AAAATTTCAT GAGCGATGGT ACTTCCCAGC CAATGCCACT 
CTATATATTG TGGGAGATAT TGACAACATA CCTCGGATAG TCCACAATAT TGAAGCTGTG 
TTTGGAAAAA ATGGTTTGGA TAATGAATCA ACACCCAGTT CTCCTAGTCC TGGTGCCTTC 
GGTGCCATGG CTAATTTTCT CGTTCCAAAG CTGCCAGCTG GTCTTGGTGG TACCTTTTCA 
AATGAGAAAA CAAATACTGC TGATCAGTCT AAGATGATTA AAAGAGAAAG ACATGCAATT 
CGTCCCCCGG TTGAGCATAA TTGGTCGCTT CCTGGAACAA GTGTTGATCT AAAGCCTCCA 
CAGATATTTA AGCACGAGCT TCTTCAGAAT TTTGCAATCA ACATGTTTTG CAAGATACCT 
GTTAGCAAGG TTCAGACATT TGGAGACCTG CGAAATGTTC TGATGAAAAG AATATTTCTC 
TCTGCTCTAC ATTTTCGAAT TAATACGAGA TACAAGAGTT CAAACCCACC ATTTACGTCT 
GTTGAACTGG ACCATAGTGA TTCGGGAAGG GAAGGATGCA CTGTTACGAC TCTTACTGTC 
ACCGCAGAGC CCCAAAATTG GCAAAATGCA GTCAAAGTTG CTGTCCAAGA GGTTCGAAGA 
CTTAAAGAAT TTGGTGTCAC CAGGGGTGAA CTTACACGTT ACATGGATGC TCTTCTAAAA 
GATAGTGAAC ATCTTGCAGC TATGATAGAT AATGTGTCTT CTGTTGATAA CCTGGATTTT 
ATCATGGAAA GTGATGCACT AAGCCACACG GTTATGGATC AGACGCAAGG ACATGAGACC 
TTGGTTGCTG TAGCTGGAAC TGTTACTCTT GAAGAGGTAA ACACTGTTGG CGCCAAGGTG 
CTAGAATTTA TTTCTGATTT CGGAAGACCC ACTGCCCCTC TTCCCGCAGC AATAGTTGCT 
TGTGTTCCCA CTAAGGTGCA TGTTGATGGG GTTGGTGAGA GTGATTTCAA TATATCTCCT 
GATGAAATAA TAGAGTCTGT CAAATCAGGA TTATTAGCAC CCATTGAAGC TGAGCCTGAG 
CTCGAGGTGC CAAAAGAATT GATCTCTCAA TCACAATTGA AAGAGTTAAC CTTGCAGAGA 
AATCCATGTT TTGTCCCTAT TCCTGGGTCA GGTTTAACCA AATTGCATGA CAAAGAGACT 
GGGATCACTC AGCTTCGGTT ATCCAATGGG ATAGCTGTAA ATTACAAGAA ATCTACGACC 
GAATCCCGGG CAGGTGTCAT GCGGCTCATT GTTGGTGGTG GACGAGCTGC TGAAACATCT 
GATTCCAAAG GAGCTGTGGT TGTTGGTGTT CGTACACTCA GCGAGGGTGG TCGAGTTGGA 
GACTTTTCAA GGGAGCAGGT GGAGCTGTTC TGTGTAAATC ATTTGATAAA CTGTTCGTTG 
GAGTCAACTG AGGAGTTCAT TGCCATGGAA TTCCGGTTTA CTCTGAGAGA CAATGGAATG 
CAAGCGGCTT TTCAGCTGCT TCACATGGTG CTTGAGCGTA GTGTCTGGTT GGAAGATGCA 
TTTGATCGAG CAAGGCAGCT ATATCTTTCG TATTTTCGAT CAATTCCTAA AAGCTTGGAG 
CGTGCGACTG CTCATAAACT TATGATCGCT ATGCTGAACG GTGATGAGCG TTTTGTTGAG 
CCGACACCTA AGTCACTACA GAGCTTGAAT CTAGAATCTG TGAAAGATGC GGTCATGAGT 
CATTTTGTTG GGGACAATAT GGAGGTAAGC ATTGTGGGGG ACTTTTCAGA GGAGGAAATC 
GAGCGTTGCA TCCTTGATTA CCTTGGTACA GTTAAAGCTA GCCATGATTC TGCAAAACCA 
CCAGGATCTG AGCCTATCTT GTTTCGCCAA CCTACAGCTG GCTTGCAATT TCAACAGGTA 
TTCTTGAAGG ATACTGATGA GAGAGCATGT GCATACATTG CGGGACCAGC GCCAAATCGA 
TGGGGCTTTA CAGTTGACGG GGATGACCTT TTTCAGTCTG TTAGCAAACT TCCTGTTGCT 
CATGATGGAC TTTTGAAATC GGAGGAACAA TTGCTAGAGG GAGGAGACAG GGAGTTGCAA 
AAGAAGCTTC GTGCCCACCC TCTTTTCTTT GGAGTTACAA TGGGTCTTCT GGCCGAAATC 
ATAAACTCAA GGCTTTTCAC GACAGTAAGA GATTCTCTTG GACTGACATA TGACGTATCA 
TTTGAGCTAA ACCTATTTGA TAGGCTGAAT CTCGGGTGGT ATGTGATATC TGTGACTTCA 
ACTCCCGGCA AGGTATACAA AGCGGTTGAT GCCTGTAAGA GTGTTCTTAG AGGTTTGCAT 
AGCAACCAAA TTGCCCCTAG GGAGTTGGAT AGGGCAAAGC GAACTCTTCT AATGAGACAT 
GAAGCTGAAC TCAAGTCCAA TGCCTATTGG CTTAATCTAT TAGCTCACTT GCAGGCCTCC 
TCTGTTCCGA GGAAGGAACT GTCATGCATT AAAGAACTTG TTTCATTGTA TGAAGCTGCT 
TCGATCGAGG ACATATACCT AGCTTATAAT CAATTAAGAG TGGACGAGGA CTCTCTGTAC 
TCCTGTATTG GTATTGCTGG GGCACAGGCG GGTGAAGAGA TTACTGTTCT CTCAGAAGAG 
GAAGAACCAG AAGATGTTTT CTCAGGAGTT GTTCCTGTGG GACGAGGCTC ATCGATGACC 
ACAAGACCAA CAACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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SPP cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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