CLC-A cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLC-A
Entrez Gene ID	834090
Full Name	chloride channel A
Synonyms	ATCLC-A,ATCLCA,CHLORIDE CHANNEL A,CHLORIDE CHANNEL-A,CLCA,chloride channel A
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_123454.3	NP_198905.1	chloride channel A
NM_001036913.2	NP_001031990.1	chloride channel A

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os12g0438600	NP_001066692.1
Arabidopsis thaliana (thale cress)	CLC-A	NP_198905.1
Arabidopsis thaliana (thale cress)	CLC-B	NP_189353.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phosphorylation of Thr(38) in the amino-terminal cytoplasmic domain of AtCLCa by OST1 increased the outward anion fluxes across the vacuolar membrane, which are essential for stomatal closure.
Title: Phosphorylation of the vacuolar anion exchanger AtCLCa is required for the stomatal response to abscisic acid.

Data highlight the importance of the proline in AtCLCa for nitrate metabolism in Arabidopsis.
Title: The proline 160 in the selectivity filter of the Arabidopsis NO(3)(-)/H(+) exchanger AtCLCa is essential for nitrate accumulation in planta.

Data show that ADP and AMP do not decrease the AtCLCa transport activity; nonetheless, AMP (but not ADP) competes with ATP, preventing inhibition.
Title: ATP binding to the C terminus of the Arabidopsis thaliana nitrate/proton antiporter, AtCLCa, regulates nitrate transport into plant vacuoles.

insertion mutants within the AtClCa and AtClCe genes revealed...a lower endogenous nitrate content; a higher nitrite content; a reduced nitrate influx into the root; and a decreased expression of several genes encoding nitrate transporters.
Title: Two anion transporters AtClCa and AtClCe fulfil interconnecting but not redundant roles in nitrate assimilation pathways.

Arabidopsis thaliana CLCa (AtCLCa) is localized to an intracellular membrane, the tonoplast of the plant vacuole; direct evidence for its anion transport ability
Title: The nitrate/proton antiporter AtCLCa mediates nitrate accumulation in plant vacuoles.

The following CLC-A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLC-A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb20189	NM_001036913.2 Latest version!	Arabidopsis thaliana chloride channel A (CLC-A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb20190	NM_123454.3 Latest version!	Arabidopsis thaliana chloride channel A (CLC-A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLC-A ( NM_001036913.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001031990.1 Arabidopsis thaliana chloride channel A (CLC-A), mRNA.

Price & Availability↑

CloneID	OAb20189
Clone ID Related Accession (Same CDS sequence)	NM_001036913.2
Accession Version	NM_001036913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	chloride channel A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_001036913.1.

ATGGTCTTTA CGGGGGCGAA TTTGGGTCTG ACTTTGGTGG CGACAGTACT TGTTGTTTAC 
TTTGCTCCTA CGGCGGCTGG TCCGGGGATC CCTGAGATTA AAGCTTACCT TAATGGCATT 
GACACTCCCA ATATGTTTGG TTTTACCACC ATGATGGTTA AGATTGTTGG AAGTATTGGA 
GCGGTTGCAG CTGGACTTGA TCTTGGTAAA GAAGGGCCAT TGGTTCATAT TGGAAGTTGC 
ATAGCTTCTT TGCTTGGTCA AGGTGGTCCA GATAATCACA GAATCAAATG GAGATGGCTT 
CGTTACTTCA ACAACGATAG AGATCGAAGA GATCTTATTA CATGTGGATC TGCCTCTGGA 
GTATGTGCTG CTTTCAGGTC ACCAGTAGGA GGAGTACTTT TCGCTCTTGA GGAAGTCGCT 
ACGTGGTGGA GAAGCGCTCT CTTGTGGAGA ACATTCTTCA GTACAGCCGT TGTGGTGGTT 
GTATTAAGAG CGTTCATTGA GATTTGTAAC TCCGGGAAAT GCGGACTTTT CGGCTCAGGA 
GGACTCATTA TGTTCGATGT GAGTCATGTA GAGGTTAGGT ACCACGCAGC AGATATAATC 
CCAGTCACAT TGATTGGAGT CTTTGGTGGC ATTCTTGGAA GCTTGTACAA TCATCTTCTG 
CATAAAGTTC TCCGGCTTTA CAATCTCATC AACCAGAAGG GAAAGATTCA TAAGGTGCTT 
CTAAGTCTTG GGGTATCTCT ATTCACATCA GTTTGCTTGT TCGGTCTTCC TTTCTTAGCG 
GAATGCAAGC CTTGTGATCC TTCTATAGAC GAGATATGTC CAACAAACGG AAGATCAGGA 
AATTTCAAGC AATTCAACTG TCCAAATGGT TATTACAACG ATCTATCCAC TCTGCTTCTC 
ACAACCAATG ATGATGCAGT CAGAAACATT TTCTCTTCAA ACACTCCTAA TGAGTTTGGC 
ATGGTTTCTC TCTGGATATT CTTTGGCCTC TACTGCATTT TGGGTCTTAT AACATTCGGT 
ATAGCGACAC CGTCGGGTCT CTTCCTACCG ATTATCCTCA TGGGTTCTGC ATACGGTAGG 
ATGCTAGGCA CAGCAATGGG GTCTTACACA AACATAGATC AAGGGCTTTA CGCGGTTCTT 
GGTGCAGCTT CACTCATGGC TGGTTCAATG AGAATGACTG TTTCACTCTG TGTTATATTT 
CTGGAACTCA CCAACAACCT TCTTTTGTTG CCCATTACAA TGTTTGTGCT TCTGATAGCT 
AAAACAGTAG GTGACAGCTT CAATCTAAGT ATCTACGAGA TCATTCTCCA TCTTAAGGGC 
TTACCTTTCT TGGAAGCAAA TCCAGAGCCG TGGATGAGGA ATCTCACTGT AGGTGAGCTT 
AACGACGCTA AGCCTCCGGT TGTAACACTT AACGGAGTAG AAAAAGTGGC AAATATAGTC 
GATGTACTAA GGAACACAAC GCATAACGCA TTCCCAGTCT TGGATGGAGC AGATCAAAAC 
ACTGGCACAG AGCTCCATGG TCTGATCCTA AGAGCTCACC TTGTGAAAGT TCTGAAAAAG 
AGATGGTTCT TGAATGAGAA AAGGAGAACA GAAGAGTGGG AAGTTAGGGA AAAGTTCACA 
CCGGTTGAAT TAGCTGAGAG AGAAGATAAC TTCGATGATG TTGCAATCAC AAGCTCAGAG 
ATGCAATTGT ATGTTGATCT TCATCCTTTG ACCAACACTA CACCTTACAC AGTGGTGCAA 
AGTATGTCAG TGGCTAAAGC TTTGGTTCTG TTCCGGTCAG TGGGTCTCAG ACATTTACTG 
GTGGTTCCCA AGATTCAAGC TTCAGGGATG TCACCTGTGA TAGGGATCTT AACAAGGCAA 
GATCTCAGGG CTTATAACAT TCTACAAGCA TTTCCTCACT TGGATAAACA CAAAAGTGGA 
AAAGCTAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001031990.1
CDS	592..2523
Translation	MVFTGANLGLTLVATVLVVYFAPTAAGPGIPEIKAYLNGIDTPNMFGFTTMMVKIVGSIGAVAAGLDLGKEGPLVHIGSCIASLLGQGGPDNHRIKWRWLRYFNNDRDRRDLITCGSASGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLRAFIEICNSGKCGLFGSGGLIMFDVSHVEVRYHAADIIPVTLIGVFGGILGSLYNHLLHKVLRLYNLINQKGKIHKVLLSLGVSLFTSVCLFGLPFLAECKPCDPSIDEICPTNGRSGNFKQFNCPNGYYNDLSTLLLTTNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLFLPIILMGSAYGRMLGTAMGSYTNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIVDVLRNTTHNAFPVLDGADQNTGTELHGLILRAHLVKVLKKRWFLNEKRRTEEWEVREKFTPVELAEREDNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSMSVAKALVLFRSVGLRHLLVVPKIQASGMSPVIGILTRQDLRAYNILQAFPHLDKHKSGKAR

Target ORF information:

RefSeq Version	NM_001036913.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana chloride channel A (CLC-A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001036913.2

ORF Insert Sequence:

ATGGTCTTTA CGGGGGCGAA TTTGGGTCTG ACTTTGGTGG CGACAGTACT TGTTGTTTAC 
TTTGCTCCTA CGGCGGCTGG TCCGGGGATC CCTGAGATTA AAGCTTACCT TAATGGCATT 
GACACTCCCA ATATGTTTGG TTTTACCACC ATGATGGTTA AGATTGTTGG AAGTATTGGA 
GCGGTTGCAG CTGGACTTGA TCTTGGTAAA GAAGGGCCAT TGGTTCATAT TGGAAGTTGC 
ATAGCTTCTT TGCTTGGTCA AGGTGGTCCA GATAATCACA GAATCAAATG GAGATGGCTT 
CGTTACTTCA ACAACGATAG AGATCGAAGA GATCTTATTA CATGTGGATC TGCCTCTGGA 
GTATGTGCTG CTTTCAGGTC ACCAGTAGGA GGAGTACTTT TCGCTCTTGA GGAAGTCGCT 
ACGTGGTGGA GAAGCGCTCT CTTGTGGAGA ACATTCTTCA GTACAGCCGT TGTGGTGGTT 
GTATTAAGAG CGTTCATTGA GATTTGTAAC TCCGGGAAAT GCGGACTTTT CGGCTCAGGA 
GGACTCATTA TGTTCGATGT GAGTCATGTA GAGGTTAGGT ACCACGCAGC AGATATAATC 
CCAGTCACAT TGATTGGAGT CTTTGGTGGC ATTCTTGGAA GCTTGTACAA TCATCTTCTG 
CATAAAGTTC TCCGGCTTTA CAATCTCATC AACCAGAAGG GAAAGATTCA TAAGGTGCTT 
CTAAGTCTTG GGGTATCTCT ATTCACATCA GTTTGCTTGT TCGGTCTTCC TTTCTTAGCG 
GAATGCAAGC CTTGTGATCC TTCTATAGAC GAGATATGTC CAACAAACGG AAGATCAGGA 
AATTTCAAGC AATTCAACTG TCCAAATGGT TATTACAACG ATCTATCCAC TCTGCTTCTC 
ACAACCAATG ATGATGCAGT CAGAAACATT TTCTCTTCAA ACACTCCTAA TGAGTTTGGC 
ATGGTTTCTC TCTGGATATT CTTTGGCCTC TACTGCATTT TGGGTCTTAT AACATTCGGT 
ATAGCGACAC CGTCGGGTCT CTTCCTACCG ATTATCCTCA TGGGTTCTGC ATACGGTAGG 
ATGCTAGGCA CAGCAATGGG GTCTTACACA AACATAGATC AAGGGCTTTA CGCGGTTCTT 
GGTGCAGCTT CACTCATGGC TGGTTCAATG AGAATGACTG TTTCACTCTG TGTTATATTT 
CTGGAACTCA CCAACAACCT TCTTTTGTTG CCCATTACAA TGTTTGTGCT TCTGATAGCT 
AAAACAGTAG GTGACAGCTT CAATCTAAGT ATCTACGAGA TCATTCTCCA TCTTAAGGGC 
TTACCTTTCT TGGAAGCAAA TCCAGAGCCG TGGATGAGGA ATCTCACTGT AGGTGAGCTT 
AACGACGCTA AGCCTCCGGT TGTAACACTT AACGGAGTAG AAAAAGTGGC AAATATAGTC 
GATGTACTAA GGAACACAAC GCATAACGCA TTCCCAGTCT TGGATGGAGC AGATCAAAAC 
ACTGGCACAG AGCTCCATGG TCTGATCCTA AGAGCTCACC TTGTGAAAGT TCTGAAAAAG 
AGATGGTTCT TGAATGAGAA AAGGAGAACA GAAGAGTGGG AAGTTAGGGA AAAGTTCACA 
CCGGTTGAAT TAGCTGAGAG AGAAGATAAC TTCGATGATG TTGCAATCAC AAGCTCAGAG 
ATGCAATTGT ATGTTGATCT TCATCCTTTG ACCAACACTA CACCTTACAC AGTGGTGCAA 
AGTATGTCAG TGGCTAAAGC TTTGGTTCTG TTCCGGTCAG TGGGTCTCAG ACATTTACTG 
GTGGTTCCCA AGATTCAAGC TTCAGGGATG TCACCTGTGA TAGGGATCTT AACAAGGCAA 
GATCTCAGGG CTTATAACAT TCTACAAGCA TTTCCTCACT TGGATAAACA CAAAAGTGGA 
AAAGCTAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLC-A ( NM_123454.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_198905.1 Arabidopsis thaliana chloride channel A (CLC-A), mRNA.

Price & Availability↑

CloneID	OAb20190
Clone ID Related Accession (Same CDS sequence)	NM_123454.3
Accession Version	NM_123454.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	chloride channel A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_123454.2.

ATGGATGAAG ATGGAAACTT GCAGATTAGT AATAGTAATT ACAATGGAGA AGAAGAAGGA 
GAAGATCCAG AGAACAACAC TTTGAATCAA CCACTGCTTA AGAGACATAG AACTCTTTCT 
TCAACTCCTC TTGCTTTGGT CGGTGCCAAG GTTTCACACA TCGAGAGTTT AGATTATGAA 
ATAAACGAGA ATGATCTGTT CAAGCATGAC TGGAGAAGCA GATCAAAAGC ACAAGTGTTT 
CAATACATAT TCTTAAAATG GACATTAGCT TGTCTTGTTG GTCTCTTCAC TGGTCTTATC 
GCTACTCTCA TCAACCTTGC TGTCGAAAAC ATCGCCGGTT ACAAACTTCT CGCCGTCGGC 
TACTACATTG CTCAAGATAG GTTTTGGACA GGTCTGATGG TCTTTACGGG GGCGAATTTG 
GGTCTGACTT TGGTGGCGAC AGTACTTGTT GTTTACTTTG CTCCTACGGC GGCTGGTCCG 
GGGATCCCTG AGATTAAAGC TTACCTTAAT GGCATTGACA CTCCCAATAT GTTTGGTTTT 
ACCACCATGA TGGTTAAGAT TGTTGGAAGT ATTGGAGCGG TTGCAGCTGG ACTTGATCTT 
GGTAAAGAAG GGCCATTGGT TCATATTGGA AGTTGCATAG CTTCTTTGCT TGGTCAAGGT 
GGTCCAGATA ATCACAGAAT CAAATGGAGA TGGCTTCGTT ACTTCAACAA CGATAGAGAT 
CGAAGAGATC TTATTACATG TGGATCTGCC TCTGGAGTAT GTGCTGCTTT CAGGTCACCA 
GTAGGAGGAG TACTTTTCGC TCTTGAGGAA GTCGCTACGT GGTGGAGAAG CGCTCTCTTG 
TGGAGAACAT TCTTCAGTAC AGCCGTTGTG GTGGTTGTAT TAAGAGCGTT CATTGAGATT 
TGTAACTCCG GGAAATGCGG ACTTTTCGGC TCAGGAGGAC TCATTATGTT CGATGTGAGT 
CATGTAGAGG TTAGGTACCA CGCAGCAGAT ATAATCCCAG TCACATTGAT TGGAGTCTTT 
GGTGGCATTC TTGGAAGCTT GTACAATCAT CTTCTGCATA AAGTTCTCCG GCTTTACAAT 
CTCATCAACC AGAAGGGAAA GATTCATAAG GTGCTTCTAA GTCTTGGGGT ATCTCTATTC 
ACATCAGTTT GCTTGTTCGG TCTTCCTTTC TTAGCGGAAT GCAAGCCTTG TGATCCTTCT 
ATAGACGAGA TATGTCCAAC AAACGGAAGA TCAGGAAATT TCAAGCAATT CAACTGTCCA 
AATGGTTATT ACAACGATCT ATCCACTCTG CTTCTCACAA CCAATGATGA TGCAGTCAGA 
AACATTTTCT CTTCAAACAC TCCTAATGAG TTTGGCATGG TTTCTCTCTG GATATTCTTT 
GGCCTCTACT GCATTTTGGG TCTTATAACA TTCGGTATAG CGACACCGTC GGGTCTCTTC 
CTACCGATTA TCCTCATGGG TTCTGCATAC GGTAGGATGC TAGGCACAGC AATGGGGTCT 
TACACAAACA TAGATCAAGG GCTTTACGCG GTTCTTGGTG CAGCTTCACT CATGGCTGGT 
TCAATGAGAA TGACTGTTTC ACTCTGTGTT ATATTTCTGG AACTCACCAA CAACCTTCTT 
TTGTTGCCCA TTACAATGTT TGTGCTTCTG ATAGCTAAAA CAGTAGGTGA CAGCTTCAAT 
CTAAGTATCT ACGAGATCAT TCTCCATCTT AAGGGCTTAC CTTTCTTGGA AGCAAATCCA 
GAGCCGTGGA TGAGGAATCT CACTGTAGGT GAGCTTAACG ACGCTAAGCC TCCGGTTGTA 
ACACTTAACG GAGTAGAAAA AGTGGCAAAT ATAGTCGATG TACTAAGGAA CACAACGCAT 
AACGCATTCC CAGTCTTGGA TGGAGCAGAT CAAAACACTG GCACAGAGCT CCATGGTCTG 
ATCCTAAGAG CTCACCTTGT GAAAGTTCTG AAAAAGAGAT GGTTCTTGAA TGAGAAAAGG 
AGAACAGAAG AGTGGGAAGT TAGGGAAAAG TTCACACCGG TTGAATTAGC TGAGAGAGAA 
GATAACTTCG ATGATGTTGC AATCACAAGC TCAGAGATGC AATTGTATGT TGATCTTCAT 
CCTTTGACCA ACACTACACC TTACACAGTG GTGCAAAGTA TGTCAGTGGC TAAAGCTTTG 
GTTCTGTTCC GGTCAGTGGG TCTCAGACAT TTACTGGTGG TTCCCAAGAT TCAAGCTTCA 
GGGATGTCAC CTGTGATAGG GATCTTAACA AGGCAAGATC TCAGGGCTTA TAACATTCTA 
CAAGCATTTC CTCACTTGGA TAAACACAAA AGTGGAAAAG CTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_198905.1
CDS	426..2753
Translation	MDEDGNLQISNSNYNGEEEGEDPENNTLNQPLLKRHRTLSSTPLALVGAKVSHIESLDYEINENDLFKHDWRSRSKAQVFQYIFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVGYYIAQDRFWTGLMVFTGANLGLTLVATVLVVYFAPTAAGPGIPEIKAYLNGIDTPNMFGFTTMMVKIVGSIGAVAAGLDLGKEGPLVHIGSCIASLLGQGGPDNHRIKWRWLRYFNNDRDRRDLITCGSASGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLRAFIEICNSGKCGLFGSGGLIMFDVSHVEVRYHAADIIPVTLIGVFGGILGSLYNHLLHKVLRLYNLINQKGKIHKVLLSLGVSLFTSVCLFGLPFLAECKPCDPSIDEICPTNGRSGNFKQFNCPNGYYNDLSTLLLTTNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLFLPIILMGSAYGRMLGTAMGSYTNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIVDVLRNTTHNAFPVLDGADQNTGTELHGLILRAHLVKVLKKRWFLNEKRRTEEWEVREKFTPVELAEREDNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSMSVAKALVLFRSVGLRHLLVVPKIQASGMSPVIGILTRQDLRAYNILQAFPHLDKHKSGKAR

Target ORF information:

RefSeq Version	NM_123454.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana chloride channel A (CLC-A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_123454.3

ORF Insert Sequence:

ATGGATGAAG ATGGAAACTT GCAGATTAGT AATAGTAATT ACAATGGAGA AGAAGAAGGA 
GAAGATCCAG AGAACAACAC TTTGAATCAA CCACTGCTTA AGAGACATAG AACTCTTTCT 
TCAACTCCTC TTGCTTTGGT CGGTGCCAAG GTTTCACACA TCGAGAGTTT AGATTATGAA 
ATAAACGAGA ATGATCTGTT CAAGCATGAC TGGAGAAGCA GATCAAAAGC ACAAGTGTTT 
CAATACATAT TCTTAAAATG GACATTAGCT TGTCTTGTTG GTCTCTTCAC TGGTCTTATC 
GCTACTCTCA TCAACCTTGC TGTCGAAAAC ATCGCCGGTT ACAAACTTCT CGCCGTCGGC 
TACTACATTG CTCAAGATAG GTTTTGGACA GGTCTGATGG TCTTTACGGG GGCGAATTTG 
GGTCTGACTT TGGTGGCGAC AGTACTTGTT GTTTACTTTG CTCCTACGGC GGCTGGTCCG 
GGGATCCCTG AGATTAAAGC TTACCTTAAT GGCATTGACA CTCCCAATAT GTTTGGTTTT 
ACCACCATGA TGGTTAAGAT TGTTGGAAGT ATTGGAGCGG TTGCAGCTGG ACTTGATCTT 
GGTAAAGAAG GGCCATTGGT TCATATTGGA AGTTGCATAG CTTCTTTGCT TGGTCAAGGT 
GGTCCAGATA ATCACAGAAT CAAATGGAGA TGGCTTCGTT ACTTCAACAA CGATAGAGAT 
CGAAGAGATC TTATTACATG TGGATCTGCC TCTGGAGTAT GTGCTGCTTT CAGGTCACCA 
GTAGGAGGAG TACTTTTCGC TCTTGAGGAA GTCGCTACGT GGTGGAGAAG CGCTCTCTTG 
TGGAGAACAT TCTTCAGTAC AGCCGTTGTG GTGGTTGTAT TAAGAGCGTT CATTGAGATT 
TGTAACTCCG GGAAATGCGG ACTTTTCGGC TCAGGAGGAC TCATTATGTT CGATGTGAGT 
CATGTAGAGG TTAGGTACCA CGCAGCAGAT ATAATCCCAG TCACATTGAT TGGAGTCTTT 
GGTGGCATTC TTGGAAGCTT GTACAATCAT CTTCTGCATA AAGTTCTCCG GCTTTACAAT 
CTCATCAACC AGAAGGGAAA GATTCATAAG GTGCTTCTAA GTCTTGGGGT ATCTCTATTC 
ACATCAGTTT GCTTGTTCGG TCTTCCTTTC TTAGCGGAAT GCAAGCCTTG TGATCCTTCT 
ATAGACGAGA TATGTCCAAC AAACGGAAGA TCAGGAAATT TCAAGCAATT CAACTGTCCA 
AATGGTTATT ACAACGATCT ATCCACTCTG CTTCTCACAA CCAATGATGA TGCAGTCAGA 
AACATTTTCT CTTCAAACAC TCCTAATGAG TTTGGCATGG TTTCTCTCTG GATATTCTTT 
GGCCTCTACT GCATTTTGGG TCTTATAACA TTCGGTATAG CGACACCGTC GGGTCTCTTC 
CTACCGATTA TCCTCATGGG TTCTGCATAC GGTAGGATGC TAGGCACAGC AATGGGGTCT 
TACACAAACA TAGATCAAGG GCTTTACGCG GTTCTTGGTG CAGCTTCACT CATGGCTGGT 
TCAATGAGAA TGACTGTTTC ACTCTGTGTT ATATTTCTGG AACTCACCAA CAACCTTCTT 
TTGTTGCCCA TTACAATGTT TGTGCTTCTG ATAGCTAAAA CAGTAGGTGA CAGCTTCAAT 
CTAAGTATCT ACGAGATCAT TCTCCATCTT AAGGGCTTAC CTTTCTTGGA AGCAAATCCA 
GAGCCGTGGA TGAGGAATCT CACTGTAGGT GAGCTTAACG ACGCTAAGCC TCCGGTTGTA 
ACACTTAACG GAGTAGAAAA AGTGGCAAAT ATAGTCGATG TACTAAGGAA CACAACGCAT 
AACGCATTCC CAGTCTTGGA TGGAGCAGAT CAAAACACTG GCACAGAGCT CCATGGTCTG 
ATCCTAAGAG CTCACCTTGT GAAAGTTCTG AAAAAGAGAT GGTTCTTGAA TGAGAAAAGG 
AGAACAGAAG AGTGGGAAGT TAGGGAAAAG TTCACACCGG TTGAATTAGC TGAGAGAGAA 
GATAACTTCG ATGATGTTGC AATCACAAGC TCAGAGATGC AATTGTATGT TGATCTTCAT 
CCTTTGACCA ACACTACACC TTACACAGTG GTGCAAAGTA TGTCAGTGGC TAAAGCTTTG 
GTTCTGTTCC GGTCAGTGGG TCTCAGACAT TTACTGGTGG TTCCCAAGAT TCAAGCTTCA 
GGGATGTCAC CTGTGATAGG GATCTTAACA AGGCAAGATC TCAGGGCTTA TAACATTCTA 
CAAGCATTTC CTCACTTGGA TAAACACAAA AGTGGAAAAG CTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
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CLC-A cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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