DCL4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCL4
Entrez Gene ID	832154
Full Name	dicer-like 4
Synonyms	ATDCL4,DICER-LIKE 4,F5O24.210,F5O24_210,dicer-like 4
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_122039.5	NP_197532.3	dicer-like 4
NM_001203419.2	NP_001190348.1	dicer-like 4
NM_001343652.1	NP_001330078.1	dicer-like 4

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	DCL4	NP_197532.3
Oryza sativa (rice)	Os04g0509300	NP_001053278.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DCL2- and RDR6-dependent transitive silencing of SMXL4 and SMXL5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over-accumulation
Title: DCL2- and RDR6-dependent transitive silencing of SMXL4 and SMXL5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over-accumulation.

Data demonstrate that different nutrient statuses of a host plant affect the post-translational regulation of the dicing activity of DCL3 and DCL4. Phosphate deficiency inhibited DCL3, and the activity of DCL3 was directly activated by inorganic phosphate.
Title: Post-Translational Regulation of the Dicing Activities of Arabidopsis DICER-LIKE 3 and 4 by Inorganic Phosphate and the Redox State.

results suggest that the spatial orientation of the Connector helix, Platform and PAZ relative to the RNase III domains is crucial for measuring dsRNA of defined length
Title: Modeling of the catalytic core of Arabidopsis thaliana Dicer-like 4 protein and its complex with double-stranded RNA.

CMA33/XCT modulates small RNA production through regulating the transcription of Dicer-Like Genes.
Title: CMA33/XCT Regulates Small RNA Production through Modulating the Transcription of Dicer-Like Genes in Arabidopsis.

DCL2/4-dependent and -independent RNA silencing pathways are involved, and may crosstalk with basal innate immunity pathways, in host defense and in cross-protection.
Title: Genetic analyses of the FRNK motif function of Turnip mosaic virus uncover multiple and potentially interactive pathways of cross-protection.

The following DCL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb41053	NM_001343652.1 Latest version!	Arabidopsis thaliana dicer-like 4 (DCL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OAb19116	NM_122039.5 Latest version!	Arabidopsis thaliana dicer-like 4 (DCL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OAb32357	NM_001203419.2 Latest version!	Arabidopsis thaliana dicer-like 4 (DCL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCL4 ( NM_001343652.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001330078.1 Arabidopsis thaliana dicer-like 4 (DCL4), mRNA.

Price & Availability↑

CloneID	OAb41053
Clone ID Related Accession (Same CDS sequence)	NM_001343652.1
Accession Version	NM_001343652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	dicer-like 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGACTCCAC AAATACTTCT GCATAACTTA CAGCACTGTT TCATCAAGAT GGAGTGTATC 
TCCCTTCTAA TATTTGATGA GTGTCACCAT GCTCAACAAC AAAGCAACCA TCCTTATGCA 
GAAATCATGA AGGTTTTCTA TAAATCGGAA AGTTTACAAC GGCCTCGAAT ATTTGGAATG 
ACTGCATCTC CAGTTGTTGG CAAAGGGTCT TTTCAATCAG AGAATTTATC GAAAAGCATT 
AATAGCCTTG AAAATTTGCT CAATGCCAAG GTTTATTCAG TGGAAAGCAA TGTCCAGCTG 
GATGGTTTTG TTTCATCTCC TTTAGTCAAA GTATATTATT ATCGGTCAGC TTTAAGTGAT 
GCATCTCAAT CGACCATCAG ATATGAAAAC ATGCTGGAGG ACATCAAACA GCGGTGCTTG 
GCATCACTTA AGCTGCTGAT TGATACTCAT CAAACACAAA CCCTCCTAAG TATGAAAAGG 
CTTCTCAAAA GATCTCATGA TAATCTCATA TATACTCTGC TGAATCTTGG CCTCTGGGGA 
GCAATACAGG CTGCTAAAAT CCAATTGAAT AGTGACCATA ATGTACAAGA CGAGCCTGTG 
GGAAAGAATC CTAAGTCAAA GATATGTGAT ACATATCTTT CTATGGCTGC TGAGGCCCTC 
TCTTCTGGTG TTGCTAAAGA TGAGAATGCA TCTGACCTCC TCAGCTTAGC GGCGTTGAAG 
GAACCATTAT TCTCTAGAAA GCTAGTTCAA TTGATTAAGA TCCTTTCGGT ATTCAGGCTA 
GAGCCACACA TGAAATGTAT AATATTTGTC AATCGGATTG TGACTGCAAG AACATTGTCA 
TGCATACTAA ATAACTTGGA ACTGCTACGG TCTTGGAAGT CTGATTTCCT TGTTGGACTT 
AGTTCTGGAC TGAAGAGCAT GTCAAGAAGG AGTATGGAAA CAATACTTAA ACGGTTCCAA 
TCTAAAGAGC TCAATTTACT GGTTGCCACT AAAGTTGGTG AAGAAGGCCT TGATATTCAG 
ACATGCTGTC TTGTGATCCG TTATGATTTA CCAGAGACTG TTACCAGCTT CATACAGTCC 
AGAGGTCGTG CTCGAATGCC TCAGTCTGAA TATGCGTTTC TAGTGGACAG CGGAAACGAG 
AAAGAGATGG ATCTTATTGA AAATTTTAAA GTAAATGAAG ATCGAATGAA TCTAGAAATT 
ACTTACAGAA GCTCAGAGGA AACTTGTCCT AGACTTGATG AGGAGTTATA CAAAGTTCAT 
GAGACAGGAG CTTGTATCAG TGGTGGAAGC AGCATCTCCC TTCTCTATAA ATATTGTTCT 
AGGCTTCCAC ATGATGAATT TTTTCAGCCC AAGCCAGAGT TTCAATTCAA GCCTGTTGAC 
GAATTTGGTG GAACTATCTG TCGCATAACT TTACCTGCTA ATGCTCCTAT AAGTGAAATC 
GAAAGTTCAC TACTACCTTC GACAGAAGCT GCTAAAAAGG ATGCTTGTCT AAAGGCTGTG 
CATGAGTTGC ACAACTTGGG TGTACTTAAC GATTTTCTGT TGCCAGATTC CAAGGATGAA 
ATTGAGGACG AATTGTCAGA TGATGAATTT GATTTTGATA ACATCAAAGG TGAAGGCTGT 
TCACGAGGTG ACCTGTATGA GATGCGTGTA CCAGTCTTGT TTAAACAAAA GTGGGATCCA 
TCTACAAGTT GTGTCAATCT TCATTCTTAC TATATAATGT TTGTGCCTCA TCCCGCTGAT 
AGGATCTACA AAAAGTTTGG TTTCTTCATG AAGTCACCTC TTCCCGTTGA GGCTGAGACT 
ATGGATATCG ATCTTCACCT TGCTCATCAA AGATCTGTAA GTGTAAAGAT TTTTCCATCA 
GGGGTCACAG AATTCGACAA CGATGAGATA AGACTAGCTG AGCTTTTCCA GGAGATTGCC 
CTGAAGGTTC TTTTTGAACG GGGGGAGCTG ATCCCGGACT TTGTTCCCTT GGAACTGCAA 
GACTCTTCTA GAACAAGCAA ATCCACCTTC TACCTTCTTC TTCCACTCTG TCTGCATGAT 
GGAGAAAGTG TTATATCTGT AGATTGGGTG ACTATCAGAA ACTGCTTGTC ATCACCAATC 
TTTAAGACTC CATCTGTTTT AGTGGAAGAT ATATTTCCTC CTTCGGGCTC TCATTTAAAG 
CTAGCAAATG GCTGCTGGAA TATTGATGAT GTGAAGAACA GCTTGGTTTT TACAACCTAC 
AGTAAACAAT TTTACTTTGT TGCTGATATC TGCCATGGAA GAAATGGTTT CAGTCCTGTT 
AAGGAATCTA GCACCAAAAG CCATGTGGAG AGCATATATA AGTTGTATGG CGTGGAACTC 
AAGCATCCTG CACAGCCACT CTTGCGTGTG AAACCACTTT GTCATGTTCG GAACTTGCTT 
CACAACCGAA TGCAGACGAA TTTGGAACCA CAAGAACTTG ACGAATACTT CATAGAGATT 
CCTCCCGAAC TTTCTCACTT AAAGATAAAA GGATTATCTA AAGACATCGG AAGCTCGTTA 
TCCTTGTTAC CATCAATCAT GCATCGTATG GAGAATTTAC TCGTGGCTAT TGAACTGAAA 
CATGTGCTGT CTGCTTCGAT CCCTGAGATA GCTGAAGTTT CTGGTCACAG GGTACTCGAG 
GCGCTCACAA CAGAGAAATG TCATGAGCGC CTTTCTCTTG AAAGGCTTGA GGTGCTTGGT 
GATGCATTCC TCAAGTTTGC TGTTAGCCGA CACCTTTTTC TACACCATGA TAGTCTTGAT 
GAAGGAGAGT TGACTCGGAG ACGCTCTAAC GTTGTTAACA ATTCCAACTT GTGCAGGCTT 
GCAATAAAAA AAAATCTGCA GGTCTACATC CGTGATCAAG CATTGGATCC TACTCAGTTC 
TTTGCATTTG GCCATCCATG CAGAGTAACC TGTGACGAGG TAGCCAGTAA AGAGGTTCAT 
TCCTTGAATA GGGATCTTGG GATCTTGGAG TCAAATACTG GTGAAATCAG ATGTAGCAAA 
GGCCATCATT GGTTGTACAA GAAAACAATT GCTGATGTGG TTGAGGCTCT TGTGGGAGCT 
TTCTTAGTTG ACAGTGGCTT CAAAGGTGCT GTGAAATTTC TGAAGTGGAT TGGTGTAAAT 
GTTGATTTTG AATCCTTGCA AGTACAAGAT GCTTGTATTG CAAGCAGGCG CTACTTGCCC 
CTCACTACTC GCAATAATTT GGAGACCCTT GAAAACCAGC TTGACTATAA GTTCCTCCAC 
AAAGGTCTAC TTGTACAAGC CTTTATCCAT CCATCTTACA ACAGGCATGG AGGAGGCTGC 
TACCAGAGAT TGGAGTTTCT TGGGGATGCT GTTCTGGACT ACTTGATGAC ATCCTATTTT 
TTCACAGTCT TCCCGAAACT GAAACCTGGT CAACTGACCG ATCTAAGATC TCTCTCAGTA 
AATAATGAGG CGCTAGCAAA TGTTGCTGTC AGTTTTTCGC TAAAGAGATT TCTATTTTGC 
GAGTCCATTT ATCTTCATGA AGTTATAGAG GATTATACCA ATTTCCTGGC ATCTTCCCCA 
TTGGCAAGTG GACAATCTGA AGGTCCAAGA TGCCCAAAGG TTCTTGGTGA CTTGGTAGAA 
TCCTGTTTGG GGGCTCTTTT CCTCGATTGT GGGTTCAACT TGAATCATGT CTGGACTATG 
ATGCTATCAT TTCTAGATCC GGTCAAAAAC TTGTCTAACC TTCAGATTAG TCCTATAAAA 
GAACTGATTG AACTTTGCCA GTCTTACAAG TGGGATCGGG AAATATCAGC GACGAAAAAG 
GATGGTGCTT TTACTGTTGA ACTAAAAGTG ACCAAGAATG GTTGTTGCCT TACAGTTTCT 
GCAACTGGTC GGAACAAAAG AGAGGGCACA AAAAAGGCTG CACAGCTGAT GATTACAAAC 
CTGAAGGCTC ATGAGAACAT AACAACCTCC CATCCGTTGG AGGATGTTCT GAAGAATGGC 
ATCCGAAATG AAGCTAAATT AATTGGCTAC AATGAAGATC CTATAGATGT TGTGGATCTT 
GTTGGGCTGG ACGTTGAAAA CCTAAATATC CTAGAAACTT TTGGCGGGAA TAGTGAAAGA 
AGCAGCTCAT ACGTCATCAG ACGAGGTCTC CCCCAAGCAC CATCTAAAAC AGAAGACAGG 
CTTCCTCAAA AGGCCATCAT AAAAGCAGGT GGACCAAGCA GCAAAACCGC AAAATCCCTC 
TTGCACGAAA CATGTGTTGC TAACTGTTGG AAGCCACCAC ACTTCGAATG TTGTGAAGAG 
GAAGGACCAG GCCACCTGAA ATCATTCGTC TACAAGGTAA TCCTGGAAGT TGAAGATGCG 
CCCAATATGA CATTGGAATG TTATGGTGAG GCTAGAGCAA CGAAGAAAGG TGCAGCAGAG 
CACGCTGCCC AAGCTGCTAT ATGGTGCCTC AAGCATTCTG GATTCCTTTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001330078.1
CDS	41..4474
Translation	MTPQILLHNLQHCFIKMECISLLIFDECHHAQQQSNHPYAEIMKVFYKSESLQRPRIFGMTASPVVGKGSFQSENLSKSINSLENLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENMLEDIKQRCLASLKLLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNLGLWGAIQAAKIQLNSDHNVQDEPVGKNPKSKICDTYLSMAAEALSSGVAKDENASDLLSLAALKEPLFSRKLVQLIKILSVFRLEPHMKCIIFVNRIVTARTLSCILNNLELLRSWKSDFLVGLSSGLKSMSRRSMETILKRFQSKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQSEYAFLVDSGNEKEMDLIENFKVNEDRMNLEITYRSSEETCPRLDEELYKVHETGACISGGSSISLLYKYCSRLPHDEFFQPKPEFQFKPVDEFGGTICRITLPANAPISEIESSLLPSTEAAKKDACLKAVHELHNLGVLNDFLLPDSKDEIEDELSDDEFDFDNIKGEGCSRGDLYEMRVPVLFKQKWDPSTSCVNLHSYYIMFVPHPADRIYKKFGFFMKSPLPVEAETMDIDLHLAHQRSVSVKIFPSGVTEFDNDEIRLAELFQEIALKVLFERGELIPDFVPLELQDSSRTSKSTFYLLLPLCLHDGESVISVDWVTIRNCLSSPIFKTPSVLVEDIFPPSGSHLKLANGCWNIDDVKNSLVFTTYSKQFYFVADICHGRNGFSPVKESSTKSHVESIYKLYGVELKHPAQPLLRVKPLCHVRNLLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIELKHVLSASIPEIAEVSGHRVLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLFLHHDSLDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQALDPTQFFAFGHPCRVTCDEVASKEVHSLNRDLGILESNTGEIRCSKGHHWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKWIGVNVDFESLQVQDACIASRRYLPLTTRNNLETLENQLDYKFLHKGLLVQAFIHPSYNRHGGGCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQLTDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVIEDYTNFLASSPLASGQSEGPRCPKVLGDLVESCLGALFLDCGFNLNHVWTMMLSFLDPVKNLSNLQISPIKELIELCQSYKWDREISATKKDGAFTVELKVTKNGCCLTVSATGRNKREGTKKAAQLMITNLKAHENITTSHPLEDVLKNGIRNEAKLIGYNEDPIDVVDLVGLDVENLNILETFGGNSERSSSYVIRRGLPQAPSKTEDRLPQKAIIKAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLECYGEARATKKGAAEHAAQAAIWCLKHSGFLC

Target ORF information:

RefSeq Version	NM_001343652.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana dicer-like 4 (DCL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343652.1

ORF Insert Sequence:

ATGACTCCAC AAATACTTCT GCATAACTTA CAGCACTGTT TCATCAAGAT GGAGTGTATC 
TCCCTTCTAA TATTTGATGA GTGTCACCAT GCTCAACAAC AAAGCAACCA TCCTTATGCA 
GAAATCATGA AGGTTTTCTA TAAATCGGAA AGTTTACAAC GGCCTCGAAT ATTTGGAATG 
ACTGCATCTC CAGTTGTTGG CAAAGGGTCT TTTCAATCAG AGAATTTATC GAAAAGCATT 
AATAGCCTTG AAAATTTGCT CAATGCCAAG GTTTATTCAG TGGAAAGCAA TGTCCAGCTG 
GATGGTTTTG TTTCATCTCC TTTAGTCAAA GTATATTATT ATCGGTCAGC TTTAAGTGAT 
GCATCTCAAT CGACCATCAG ATATGAAAAC ATGCTGGAGG ACATCAAACA GCGGTGCTTG 
GCATCACTTA AGCTGCTGAT TGATACTCAT CAAACACAAA CCCTCCTAAG TATGAAAAGG 
CTTCTCAAAA GATCTCATGA TAATCTCATA TATACTCTGC TGAATCTTGG CCTCTGGGGA 
GCAATACAGG CTGCTAAAAT CCAATTGAAT AGTGACCATA ATGTACAAGA CGAGCCTGTG 
GGAAAGAATC CTAAGTCAAA GATATGTGAT ACATATCTTT CTATGGCTGC TGAGGCCCTC 
TCTTCTGGTG TTGCTAAAGA TGAGAATGCA TCTGACCTCC TCAGCTTAGC GGCGTTGAAG 
GAACCATTAT TCTCTAGAAA GCTAGTTCAA TTGATTAAGA TCCTTTCGGT ATTCAGGCTA 
GAGCCACACA TGAAATGTAT AATATTTGTC AATCGGATTG TGACTGCAAG AACATTGTCA 
TGCATACTAA ATAACTTGGA ACTGCTACGG TCTTGGAAGT CTGATTTCCT TGTTGGACTT 
AGTTCTGGAC TGAAGAGCAT GTCAAGAAGG AGTATGGAAA CAATACTTAA ACGGTTCCAA 
TCTAAAGAGC TCAATTTACT GGTTGCCACT AAAGTTGGTG AAGAAGGCCT TGATATTCAG 
ACATGCTGTC TTGTGATCCG TTATGATTTA CCAGAGACTG TTACCAGCTT CATACAGTCC 
AGAGGTCGTG CTCGAATGCC TCAGTCTGAA TATGCGTTTC TAGTGGACAG CGGAAACGAG 
AAAGAGATGG ATCTTATTGA AAATTTTAAA GTAAATGAAG ATCGAATGAA TCTAGAAATT 
ACTTACAGAA GCTCAGAGGA AACTTGTCCT AGACTTGATG AGGAGTTATA CAAAGTTCAT 
GAGACAGGAG CTTGTATCAG TGGTGGAAGC AGCATCTCCC TTCTCTATAA ATATTGTTCT 
AGGCTTCCAC ATGATGAATT TTTTCAGCCC AAGCCAGAGT TTCAATTCAA GCCTGTTGAC 
GAATTTGGTG GAACTATCTG TCGCATAACT TTACCTGCTA ATGCTCCTAT AAGTGAAATC 
GAAAGTTCAC TACTACCTTC GACAGAAGCT GCTAAAAAGG ATGCTTGTCT AAAGGCTGTG 
CATGAGTTGC ACAACTTGGG TGTACTTAAC GATTTTCTGT TGCCAGATTC CAAGGATGAA 
ATTGAGGACG AATTGTCAGA TGATGAATTT GATTTTGATA ACATCAAAGG TGAAGGCTGT 
TCACGAGGTG ACCTGTATGA GATGCGTGTA CCAGTCTTGT TTAAACAAAA GTGGGATCCA 
TCTACAAGTT GTGTCAATCT TCATTCTTAC TATATAATGT TTGTGCCTCA TCCCGCTGAT 
AGGATCTACA AAAAGTTTGG TTTCTTCATG AAGTCACCTC TTCCCGTTGA GGCTGAGACT 
ATGGATATCG ATCTTCACCT TGCTCATCAA AGATCTGTAA GTGTAAAGAT TTTTCCATCA 
GGGGTCACAG AATTCGACAA CGATGAGATA AGACTAGCTG AGCTTTTCCA GGAGATTGCC 
CTGAAGGTTC TTTTTGAACG GGGGGAGCTG ATCCCGGACT TTGTTCCCTT GGAACTGCAA 
GACTCTTCTA GAACAAGCAA ATCCACCTTC TACCTTCTTC TTCCACTCTG TCTGCATGAT 
GGAGAAAGTG TTATATCTGT AGATTGGGTG ACTATCAGAA ACTGCTTGTC ATCACCAATC 
TTTAAGACTC CATCTGTTTT AGTGGAAGAT ATATTTCCTC CTTCGGGCTC TCATTTAAAG 
CTAGCAAATG GCTGCTGGAA TATTGATGAT GTGAAGAACA GCTTGGTTTT TACAACCTAC 
AGTAAACAAT TTTACTTTGT TGCTGATATC TGCCATGGAA GAAATGGTTT CAGTCCTGTT 
AAGGAATCTA GCACCAAAAG CCATGTGGAG AGCATATATA AGTTGTATGG CGTGGAACTC 
AAGCATCCTG CACAGCCACT CTTGCGTGTG AAACCACTTT GTCATGTTCG GAACTTGCTT 
CACAACCGAA TGCAGACGAA TTTGGAACCA CAAGAACTTG ACGAATACTT CATAGAGATT 
CCTCCCGAAC TTTCTCACTT AAAGATAAAA GGATTATCTA AAGACATCGG AAGCTCGTTA 
TCCTTGTTAC CATCAATCAT GCATCGTATG GAGAATTTAC TCGTGGCTAT TGAACTGAAA 
CATGTGCTGT CTGCTTCGAT CCCTGAGATA GCTGAAGTTT CTGGTCACAG GGTACTCGAG 
GCGCTCACAA CAGAGAAATG TCATGAGCGC CTTTCTCTTG AAAGGCTTGA GGTGCTTGGT 
GATGCATTCC TCAAGTTTGC TGTTAGCCGA CACCTTTTTC TACACCATGA TAGTCTTGAT 
GAAGGAGAGT TGACTCGGAG ACGCTCTAAC GTTGTTAACA ATTCCAACTT GTGCAGGCTT 
GCAATAAAAA AAAATCTGCA GGTCTACATC CGTGATCAAG CATTGGATCC TACTCAGTTC 
TTTGCATTTG GCCATCCATG CAGAGTAACC TGTGACGAGG TAGCCAGTAA AGAGGTTCAT 
TCCTTGAATA GGGATCTTGG GATCTTGGAG TCAAATACTG GTGAAATCAG ATGTAGCAAA 
GGCCATCATT GGTTGTACAA GAAAACAATT GCTGATGTGG TTGAGGCTCT TGTGGGAGCT 
TTCTTAGTTG ACAGTGGCTT CAAAGGTGCT GTGAAATTTC TGAAGTGGAT TGGTGTAAAT 
GTTGATTTTG AATCCTTGCA AGTACAAGAT GCTTGTATTG CAAGCAGGCG CTACTTGCCC 
CTCACTACTC GCAATAATTT GGAGACCCTT GAAAACCAGC TTGACTATAA GTTCCTCCAC 
AAAGGTCTAC TTGTACAAGC CTTTATCCAT CCATCTTACA ACAGGCATGG AGGAGGCTGC 
TACCAGAGAT TGGAGTTTCT TGGGGATGCT GTTCTGGACT ACTTGATGAC ATCCTATTTT 
TTCACAGTCT TCCCGAAACT GAAACCTGGT CAACTGACCG ATCTAAGATC TCTCTCAGTA 
AATAATGAGG CGCTAGCAAA TGTTGCTGTC AGTTTTTCGC TAAAGAGATT TCTATTTTGC 
GAGTCCATTT ATCTTCATGA AGTTATAGAG GATTATACCA ATTTCCTGGC ATCTTCCCCA 
TTGGCAAGTG GACAATCTGA AGGTCCAAGA TGCCCAAAGG TTCTTGGTGA CTTGGTAGAA 
TCCTGTTTGG GGGCTCTTTT CCTCGATTGT GGGTTCAACT TGAATCATGT CTGGACTATG 
ATGCTATCAT TTCTAGATCC GGTCAAAAAC TTGTCTAACC TTCAGATTAG TCCTATAAAA 
GAACTGATTG AACTTTGCCA GTCTTACAAG TGGGATCGGG AAATATCAGC GACGAAAAAG 
GATGGTGCTT TTACTGTTGA ACTAAAAGTG ACCAAGAATG GTTGTTGCCT TACAGTTTCT 
GCAACTGGTC GGAACAAAAG AGAGGGCACA AAAAAGGCTG CACAGCTGAT GATTACAAAC 
CTGAAGGCTC ATGAGAACAT AACAACCTCC CATCCGTTGG AGGATGTTCT GAAGAATGGC 
ATCCGAAATG AAGCTAAATT AATTGGCTAC AATGAAGATC CTATAGATGT TGTGGATCTT 
GTTGGGCTGG ACGTTGAAAA CCTAAATATC CTAGAAACTT TTGGCGGGAA TAGTGAAAGA 
AGCAGCTCAT ACGTCATCAG ACGAGGTCTC CCCCAAGCAC CATCTAAAAC AGAAGACAGG 
CTTCCTCAAA AGGCCATCAT AAAAGCAGGT GGACCAAGCA GCAAAACCGC AAAATCCCTC 
TTGCACGAAA CATGTGTTGC TAACTGTTGG AAGCCACCAC ACTTCGAATG TTGTGAAGAG 
GAAGGACCAG GCCACCTGAA ATCATTCGTC TACAAGGTAA TCCTGGAAGT TGAAGATGCG 
CCCAATATGA CATTGGAATG TTATGGTGAG GCTAGAGCAA CGAAGAAAGG TGCAGCAGAG 
CACGCTGCCC AAGCTGCTAT ATGGTGCCTC AAGCATTCTG GATTCCTTTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCL4 ( NM_122039.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_197532.3 Arabidopsis thaliana dicer-like 4 (DCL4), mRNA.

Price & Availability↑

CloneID	OAb19116
Clone ID Related Accession (Same CDS sequence)	NM_122039.5
Accession Version	NM_122039.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	dicer-like 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_122039.4.

ATGCGTGACG AAGTTGACTT GAGCTTGACC ATTCCCTCGA AGCTTTTGGG GAAGCGAGAC 
AGAGAACAAA AAAATTGTGA AGAAGAAAAA AACAAAAACA AAAAAGCTAA AAAGCAGCAA 
AAGGACCCAA TTCTTCTTCA CACTAGTGCT GCCACTCACA AGTTTCTTCC TCCTCCTTTG 
ACCATGCCGT ACAGTGAAAT CGGCGACGAT CTTCGCTCAC TCGACTTTGA CCACGCCGAT 
GTTTCTTCCG ACCTTCACCT CACTTCTTCT TCCTCTGTTT CTTCGTTTTC CTCTTCTTCG 
TCTTCTTTGT TCTCCGCGGC TGGTACGGAT GATCCTTCAC CGAAAATGGA GAAAGACCCT 
AGAAAAATCG CCAGGAGGTA TCAGGTGGAG CTGTGTAAGA AAGCAACGGA GGAGAACGTT 
ATTGTATATT TGGGTACAGG TTGTGGGAAG ACTCACATTG CAGTGATGCT TATATATGAG 
CTTGGTCATT TGGTTCTTAG TCCCAAGAAA AGTGTTTGTA TTTTTCTTGC TCCCACCGTG 
GCTTTGGTCG AACAGCAAGC CAAGGTCATA GCGGACTCTG TCAACTTCAA AGTTGCAATA 
CATTGTGGAG GCAAGAGGAT TGTGAAGAGC CACTCGGAGT GGGAGAGAGA GATTGCAGCG 
AATGAGGTTC TTGTTATGAC TCCACAAATA CTTCTGCATA ACTTACAGCA CTGTTTCATC 
AAGATGGAGT GTATCTCCCT TCTAATATTT GATGAGTGTC ACCATGCTCA ACAACAAAGC 
AACCATCCTT ATGCAGAAAT CATGAAGGTT TTCTATAAAT CGGAAAGTTT ACAACGGCCT 
CGAATATTTG GAATGACTGC ATCTCCAGTT GTTGGCAAAG GGTCTTTTCA ATCAGAGAAT 
TTATCGAAAA GCATTAATAG CCTTGAAAAT TTGCTCAATG CCAAGGTTTA TTCAGTGGAA 
AGCAATGTCC AGCTGGATGG TTTTGTTTCA TCTCCTTTAG TCAAAGTATA TTATTATCGG 
TCAGCTTTAA GTGATGCATC TCAATCGACC ATCAGATATG AAAACATGCT GGAGGACATC 
AAACAGCGGT GCTTGGCATC ACTTAAGCTG CTGATTGATA CTCATCAAAC ACAAACCCTC 
CTAAGTATGA AAAGGCTTCT CAAAAGATCT CATGATAATC TCATATATAC TCTGCTGAAT 
CTTGGCCTCT GGGGAGCAAT ACAGGCTGCT AAAATCCAAT TGAATAGTGA CCATAATGTA 
CAAGACGAGC CTGTGGGAAA GAATCCTAAG TCAAAGATAT GTGATACATA TCTTTCTATG 
GCTGCTGAGG CCCTCTCTTC TGGTGTTGCT AAAGATGAGA ATGCATCTGA CCTCCTCAGC 
TTAGCGGCGT TGAAGGAACC ATTATTCTCT AGAAAGCTAG TTCAATTGAT TAAGATCCTT 
TCGGTATTCA GGCTAGAGCC ACACATGAAA TGTATAATAT TTGTCAATCG GATTGTGACT 
GCAAGAACAT TGTCATGCAT ACTAAATAAC TTGGAACTGC TACGGTCTTG GAAGTCTGAT 
TTCCTTGTTG GACTTAGTTC TGGACTGAAG AGCATGTCAA GAAGGAGTAT GGAAACAATA 
CTTAAACGGT TCCAATCTAA AGAGCTCAAT TTACTGGTTG CCACTAAAGT TGGTGAAGAA 
GGCCTTGATA TTCAGACATG CTGTCTTGTG ATCCGTTATG ATTTACCAGA GACTGTTACC 
AGCTTCATAC AGTCCAGAGG TCGTGCTCGA ATGCCTCAGT CTGAATATGC GTTTCTAGTG 
GACAGCGGAA ACGAGAAAGA GATGGATCTT ATTGAAAATT TTAAAGTAAA TGAAGATCGA 
ATGAATCTAG AAATTACTTA CAGAAGCTCA GAGGAAACTT GTCCTAGACT TGATGAGGAG 
TTATACAAAG TTCATGAGAC AGGAGCTTGT ATCAGTGGTG GAAGCAGCAT CTCCCTTCTC 
TATAAATATT GTTCTAGGCT TCCACATGAT GAATTTTTTC AGCCCAAGCC AGAGTTTCAA 
TTCAAGCCTG TTGACGAATT TGGTGGAACT ATCTGTCGCA TAACTTTACC TGCTAATGCT 
CCTATAAGTG AAATCGAAAG TTCACTACTA CCTTCGACAG AAGCTGCTAA AAAGGATGCT 
TGTCTAAAGG CTGTGCATGA GTTGCACAAC TTGGGTGTAC TTAACGATTT TCTGTTGCCA 
GATTCCAAGG ATGAAATTGA GGACGAATTG TCAGATGATG AATTTGATTT TGATAACATC 
AAAGGTGAAG GCTGTTCACG AGGTGACCTG TATGAGATGC GTGTACCAGT CTTGTTTAAA 
CAAAAGTGGG ATCCATCTAC AAGTTGTGTC AATCTTCATT CTTACTATAT AATGTTTGTG 
CCTCATCCCG CTGATAGGAT CTACAAAAAG TTTGGTTTCT TCATGAAGTC ACCTCTTCCC 
GTTGAGGCTG AGACTATGGA TATCGATCTT CACCTTGCTC ATCAAAGATC TGTAAGTGTA 
AAGATTTTTC CATCAGGGGT CACAGAATTC GACAACGATG AGATAAGACT AGCTGAGCTT 
TTCCAGGAGA TTGCCCTGAA GGTTCTTTTT GAACGGGGGG AGCTGATCCC GGACTTTGTT 
CCCTTGGAAC TGCAAGACTC TTCTAGAACA AGCAAATCCA CCTTCTACCT TCTTCTTCCA 
CTCTGTCTGC ATGATGGAGA AAGTGTTATA TCTGTAGATT GGGTGACTAT CAGAAACTGC 
TTGTCATCAC CAATCTTTAA GACTCCATCT GTTTTAGTGG AAGATATATT TCCTCCTTCG 
GGCTCTCATT TAAAGCTAGC AAATGGCTGC TGGAATATTG ATGATGTGAA GAACAGCTTG 
GTTTTTACAA CCTACAGTAA ACAATTTTAC TTTGTTGCTG ATATCTGCCA TGGAAGAAAT 
GGTTTCAGTC CTGTTAAGGA ATCTAGCACC AAAAGCCATG TGGAGAGCAT ATATAAGTTG 
TATGGCGTGG AACTCAAGCA TCCTGCACAG CCACTCTTGC GTGTGAAACC ACTTTGTCAT 
GTTCGGAACT TGCTTCACAA CCGAATGCAG ACGAATTTGG AACCACAAGA ACTTGACGAA 
TACTTCATAG AGATTCCTCC CGAACTTTCT CACTTAAAGA TAAAAGGATT ATCTAAAGAC 
ATCGGAAGCT CGTTATCCTT GTTACCATCA ATCATGCATC GTATGGAGAA TTTACTCGTG 
GCTATTGAAC TGAAACATGT GCTGTCTGCT TCGATCCCTG AGATAGCTGA AGTTTCTGGT 
CACAGGGTAC TCGAGGCGCT CACAACAGAG AAATGTCATG AGCGCCTTTC TCTTGAAAGG 
CTTGAGGTGC TTGGTGATGC ATTCCTCAAG TTTGCTGTTA GCCGACACCT TTTTCTACAC 
CATGATAGTC TTGATGAAGG AGAGTTGACT CGGAGACGCT CTAACGTTGT TAACAATTCC 
AACTTGTGCA GGCTTGCAAT AAAAAAAAAT CTGCAGGTCT ACATCCGTGA TCAAGCATTG 
GATCCTACTC AGTTCTTTGC ATTTGGCCAT CCATGCAGAG TAACCTGTGA CGAGGTAGCC 
AGTAAAGAGG TTCATTCCTT GAATAGGGAT CTTGGGATCT TGGAGTCAAA TACTGGTGAA 
ATCAGATGTA GCAAAGGCCA TCATTGGTTG TACAAGAAAA CAATTGCTGA TGTGGTTGAG 
GCTCTTGTGG GAGCTTTCTT AGTTGACAGT GGCTTCAAAG GTGCTGTGAA ATTTCTGAAG 
TGGATTGGTG TAAATGTTGA TTTTGAATCC TTGCAAGTAC AAGATGCTTG TATTGCAAGC 
AGGCGCTACT TGCCCCTCAC TACTCGCAAT AATTTGGAGA CCCTTGAAAA CCAGCTTGAC 
TATAAGTTCC TCCACAAAGG TCTACTTGTA CAAGCCTTTA TCCATCCATC TTACAACAGG 
CATGGAGGAG GCTGCTACCA GAGATTGGAG TTTCTTGGGG ATGCTGTTCT GGACTACTTG 
ATGACATCCT ATTTTTTCAC AGTCTTCCCG AAACTGAAAC CTGGTCAACT GACCGATCTA 
AGATCTCTCT CAGTAAATAA TGAGGCGCTA GCAAATGTTG CTGTCAGTTT TTCGCTAAAG 
AGATTTCTAT TTTGCGAGTC CATTTATCTT CATGAAGTTA TAGAGGATTA TACCAATTTC 
CTGGCATCTT CCCCATTGGC AAGTGGACAA TCTGAAGGTC CAAGATGCCC AAAGGTTCTT 
GGTGACTTGG TAGAATCCTG TTTGGGGGCT CTTTTCCTCG ATTGTGGGTT CAACTTGAAT 
CATGTCTGGA CTATGATGCT ATCATTTCTA GATCCGGTCA AAAACTTGTC TAACCTTCAG 
ATTAGTCCTA TAAAAGAACT GATTGAACTT TGCCAGTCTT ACAAGTGGGA TCGGGAAATA 
TCAGCGACGA AAAAGGATGG TGCTTTTACT GTTGAACTAA AAGTGACCAA GAATGGTTGT 
TGCCTTACAG TTTCTGCAAC TGGTCGGAAC AAAAGAGAGG GCACAAAAAA GGCTGCACAG 
CTGATGATTA CAAACCTGAA GGCTCATGAG AACATAACAA CCTCCCATCC GTTGGAGGAT 
GTTCTGAAGA ATGGCATCCG AAATGAAGCT AAATTAATTG GCTACAATGA AGATCCTATA 
GATGTTGTGG ATCTTGTTGG GCTGGACGTT GAAAACCTAA ATATCCTAGA AACTTTTGGC 
GGGAATAGTG AAAGAAGCAG CTCATACGTC ATCAGACGAG GTCTCCCCCA AGCACCATCT 
AAAACAGAAG ACAGGCTTCC TCAAAAGGCC ATCATAAAAG CAGGTGGACC AAGCAGCAAA 
ACCGCAAAAT CCCTCTTGCA CGAAACATGT GTTGCTAACT GTTGGAAGCC ACCACACTTC 
GAATGTTGTG AAGAGGAAGG ACCAGGCCAC CTGAAATCAT TCGTCTACAA GGTAATCCTG 
GAAGTTGAAG ATGCGCCCAA TATGACATTG GAATGTTATG GTGAGGCTAG AGCAACGAAG 
AAAGGTGCAG CAGAGCACGC TGCCCAAGCT GCTATATGGT GCCTCAAGCA TTCTGGATTC 
CTTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_197532.3
CDS	199..5307
Translation	MRDEVDLSLTIPSKLLGKRDREQKNCEEEKNKNKKAKKQQKDPILLHTSAATHKFLPPPLTMPYSEIGDDLRSLDFDHADVSSDLHLTSSSSVSSFSSSSSSLFSAAGTDDPSPKMEKDPRKIARRYQVELCKKATEENVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKVAIHCGGKRIVKSHSEWEREIAANEVLVMTPQILLHNLQHCFIKMECISLLIFDECHHAQQQSNHPYAEIMKVFYKSESLQRPRIFGMTASPVVGKGSFQSENLSKSINSLENLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENMLEDIKQRCLASLKLLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNLGLWGAIQAAKIQLNSDHNVQDEPVGKNPKSKICDTYLSMAAEALSSGVAKDENASDLLSLAALKEPLFSRKLVQLIKILSVFRLEPHMKCIIFVNRIVTARTLSCILNNLELLRSWKSDFLVGLSSGLKSMSRRSMETILKRFQSKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQSEYAFLVDSGNEKEMDLIENFKVNEDRMNLEITYRSSEETCPRLDEELYKVHETGACISGGSSISLLYKYCSRLPHDEFFQPKPEFQFKPVDEFGGTICRITLPANAPISEIESSLLPSTEAAKKDACLKAVHELHNLGVLNDFLLPDSKDEIEDELSDDEFDFDNIKGEGCSRGDLYEMRVPVLFKQKWDPSTSCVNLHSYYIMFVPHPADRIYKKFGFFMKSPLPVEAETMDIDLHLAHQRSVSVKIFPSGVTEFDNDEIRLAELFQEIALKVLFERGELIPDFVPLELQDSSRTSKSTFYLLLPLCLHDGESVISVDWVTIRNCLSSPIFKTPSVLVEDIFPPSGSHLKLANGCWNIDDVKNSLVFTTYSKQFYFVADICHGRNGFSPVKESSTKSHVESIYKLYGVELKHPAQPLLRVKPLCHVRNLLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIELKHVLSASIPEIAEVSGHRVLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLFLHHDSLDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQALDPTQFFAFGHPCRVTCDEVASKEVHSLNRDLGILESNTGEIRCSKGHHWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKWIGVNVDFESLQVQDACIASRRYLPLTTRNNLETLENQLDYKFLHKGLLVQAFIHPSYNRHGGGCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQLTDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVIEDYTNFLASSPLASGQSEGPRCPKVLGDLVESCLGALFLDCGFNLNHVWTMMLSFLDPVKNLSNLQISPIKELIELCQSYKWDREISATKKDGAFTVELKVTKNGCCLTVSATGRNKREGTKKAAQLMITNLKAHENITTSHPLEDVLKNGIRNEAKLIGYNEDPIDVVDLVGLDVENLNILETFGGNSERSSSYVIRRGLPQAPSKTEDRLPQKAIIKAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLECYGEARATKKGAAEHAAQAAIWCLKHSGFLC

Target ORF information:

RefSeq Version	NM_122039.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana dicer-like 4 (DCL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_122039.5

ORF Insert Sequence:

ATGCGTGACG AAGTTGACTT GAGCTTGACC ATTCCCTCGA AGCTTTTGGG GAAGCGAGAC 
AGAGAACAAA AAAATTGTGA AGAAGAAAAA AACAAAAACA AAAAAGCTAA AAAGCAGCAA 
AAGGACCCAA TTCTTCTTCA CACTAGTGCT GCCACTCACA AGTTTCTTCC TCCTCCTTTG 
ACCATGCCGT ACAGTGAAAT CGGCGACGAT CTTCGCTCAC TCGACTTTGA CCACGCCGAT 
GTTTCTTCCG ACCTTCACCT CACTTCTTCT TCCTCTGTTT CTTCGTTTTC CTCTTCTTCG 
TCTTCTTTGT TCTCCGCGGC TGGTACGGAT GATCCTTCAC CGAAAATGGA GAAAGACCCT 
AGAAAAATCG CCAGGAGGTA TCAGGTGGAG CTGTGTAAGA AAGCAACGGA GGAGAACGTT 
ATTGTATATT TGGGTACAGG TTGTGGGAAG ACTCACATTG CAGTGATGCT TATATATGAG 
CTTGGTCATT TGGTTCTTAG TCCCAAGAAA AGTGTTTGTA TTTTTCTTGC TCCCACCGTG 
GCTTTGGTCG AACAGCAAGC CAAGGTCATA GCGGACTCTG TCAACTTCAA AGTTGCAATA 
CATTGTGGAG GCAAGAGGAT TGTGAAGAGC CACTCGGAGT GGGAGAGAGA GATTGCAGCG 
AATGAGGTTC TTGTTATGAC TCCACAAATA CTTCTGCATA ACTTACAGCA CTGTTTCATC 
AAGATGGAGT GTATCTCCCT TCTAATATTT GATGAGTGTC ACCATGCTCA ACAACAAAGC 
AACCATCCTT ATGCAGAAAT CATGAAGGTT TTCTATAAAT CGGAAAGTTT ACAACGGCCT 
CGAATATTTG GAATGACTGC ATCTCCAGTT GTTGGCAAAG GGTCTTTTCA ATCAGAGAAT 
TTATCGAAAA GCATTAATAG CCTTGAAAAT TTGCTCAATG CCAAGGTTTA TTCAGTGGAA 
AGCAATGTCC AGCTGGATGG TTTTGTTTCA TCTCCTTTAG TCAAAGTATA TTATTATCGG 
TCAGCTTTAA GTGATGCATC TCAATCGACC ATCAGATATG AAAACATGCT GGAGGACATC 
AAACAGCGGT GCTTGGCATC ACTTAAGCTG CTGATTGATA CTCATCAAAC ACAAACCCTC 
CTAAGTATGA AAAGGCTTCT CAAAAGATCT CATGATAATC TCATATATAC TCTGCTGAAT 
CTTGGCCTCT GGGGAGCAAT ACAGGCTGCT AAAATCCAAT TGAATAGTGA CCATAATGTA 
CAAGACGAGC CTGTGGGAAA GAATCCTAAG TCAAAGATAT GTGATACATA TCTTTCTATG 
GCTGCTGAGG CCCTCTCTTC TGGTGTTGCT AAAGATGAGA ATGCATCTGA CCTCCTCAGC 
TTAGCGGCGT TGAAGGAACC ATTATTCTCT AGAAAGCTAG TTCAATTGAT TAAGATCCTT 
TCGGTATTCA GGCTAGAGCC ACACATGAAA TGTATAATAT TTGTCAATCG GATTGTGACT 
GCAAGAACAT TGTCATGCAT ACTAAATAAC TTGGAACTGC TACGGTCTTG GAAGTCTGAT 
TTCCTTGTTG GACTTAGTTC TGGACTGAAG AGCATGTCAA GAAGGAGTAT GGAAACAATA 
CTTAAACGGT TCCAATCTAA AGAGCTCAAT TTACTGGTTG CCACTAAAGT TGGTGAAGAA 
GGCCTTGATA TTCAGACATG CTGTCTTGTG ATCCGTTATG ATTTACCAGA GACTGTTACC 
AGCTTCATAC AGTCCAGAGG TCGTGCTCGA ATGCCTCAGT CTGAATATGC GTTTCTAGTG 
GACAGCGGAA ACGAGAAAGA GATGGATCTT ATTGAAAATT TTAAAGTAAA TGAAGATCGA 
ATGAATCTAG AAATTACTTA CAGAAGCTCA GAGGAAACTT GTCCTAGACT TGATGAGGAG 
TTATACAAAG TTCATGAGAC AGGAGCTTGT ATCAGTGGTG GAAGCAGCAT CTCCCTTCTC 
TATAAATATT GTTCTAGGCT TCCACATGAT GAATTTTTTC AGCCCAAGCC AGAGTTTCAA 
TTCAAGCCTG TTGACGAATT TGGTGGAACT ATCTGTCGCA TAACTTTACC TGCTAATGCT 
CCTATAAGTG AAATCGAAAG TTCACTACTA CCTTCGACAG AAGCTGCTAA AAAGGATGCT 
TGTCTAAAGG CTGTGCATGA GTTGCACAAC TTGGGTGTAC TTAACGATTT TCTGTTGCCA 
GATTCCAAGG ATGAAATTGA GGACGAATTG TCAGATGATG AATTTGATTT TGATAACATC 
AAAGGTGAAG GCTGTTCACG AGGTGACCTG TATGAGATGC GTGTACCAGT CTTGTTTAAA 
CAAAAGTGGG ATCCATCTAC AAGTTGTGTC AATCTTCATT CTTACTATAT AATGTTTGTG 
CCTCATCCCG CTGATAGGAT CTACAAAAAG TTTGGTTTCT TCATGAAGTC ACCTCTTCCC 
GTTGAGGCTG AGACTATGGA TATCGATCTT CACCTTGCTC ATCAAAGATC TGTAAGTGTA 
AAGATTTTTC CATCAGGGGT CACAGAATTC GACAACGATG AGATAAGACT AGCTGAGCTT 
TTCCAGGAGA TTGCCCTGAA GGTTCTTTTT GAACGGGGGG AGCTGATCCC GGACTTTGTT 
CCCTTGGAAC TGCAAGACTC TTCTAGAACA AGCAAATCCA CCTTCTACCT TCTTCTTCCA 
CTCTGTCTGC ATGATGGAGA AAGTGTTATA TCTGTAGATT GGGTGACTAT CAGAAACTGC 
TTGTCATCAC CAATCTTTAA GACTCCATCT GTTTTAGTGG AAGATATATT TCCTCCTTCG 
GGCTCTCATT TAAAGCTAGC AAATGGCTGC TGGAATATTG ATGATGTGAA GAACAGCTTG 
GTTTTTACAA CCTACAGTAA ACAATTTTAC TTTGTTGCTG ATATCTGCCA TGGAAGAAAT 
GGTTTCAGTC CTGTTAAGGA ATCTAGCACC AAAAGCCATG TGGAGAGCAT ATATAAGTTG 
TATGGCGTGG AACTCAAGCA TCCTGCACAG CCACTCTTGC GTGTGAAACC ACTTTGTCAT 
GTTCGGAACT TGCTTCACAA CCGAATGCAG ACGAATTTGG AACCACAAGA ACTTGACGAA 
TACTTCATAG AGATTCCTCC CGAACTTTCT CACTTAAAGA TAAAAGGATT ATCTAAAGAC 
ATCGGAAGCT CGTTATCCTT GTTACCATCA ATCATGCATC GTATGGAGAA TTTACTCGTG 
GCTATTGAAC TGAAACATGT GCTGTCTGCT TCGATCCCTG AGATAGCTGA AGTTTCTGGT 
CACAGGGTAC TCGAGGCGCT CACAACAGAG AAATGTCATG AGCGCCTTTC TCTTGAAAGG 
CTTGAGGTGC TTGGTGATGC ATTCCTCAAG TTTGCTGTTA GCCGACACCT TTTTCTACAC 
CATGATAGTC TTGATGAAGG AGAGTTGACT CGGAGACGCT CTAACGTTGT TAACAATTCC 
AACTTGTGCA GGCTTGCAAT AAAAAAAAAT CTGCAGGTCT ACATCCGTGA TCAAGCATTG 
GATCCTACTC AGTTCTTTGC ATTTGGCCAT CCATGCAGAG TAACCTGTGA CGAGGTAGCC 
AGTAAAGAGG TTCATTCCTT GAATAGGGAT CTTGGGATCT TGGAGTCAAA TACTGGTGAA 
ATCAGATGTA GCAAAGGCCA TCATTGGTTG TACAAGAAAA CAATTGCTGA TGTGGTTGAG 
GCTCTTGTGG GAGCTTTCTT AGTTGACAGT GGCTTCAAAG GTGCTGTGAA ATTTCTGAAG 
TGGATTGGTG TAAATGTTGA TTTTGAATCC TTGCAAGTAC AAGATGCTTG TATTGCAAGC 
AGGCGCTACT TGCCCCTCAC TACTCGCAAT AATTTGGAGA CCCTTGAAAA CCAGCTTGAC 
TATAAGTTCC TCCACAAAGG TCTACTTGTA CAAGCCTTTA TCCATCCATC TTACAACAGG 
CATGGAGGAG GCTGCTACCA GAGATTGGAG TTTCTTGGGG ATGCTGTTCT GGACTACTTG 
ATGACATCCT ATTTTTTCAC AGTCTTCCCG AAACTGAAAC CTGGTCAACT GACCGATCTA 
AGATCTCTCT CAGTAAATAA TGAGGCGCTA GCAAATGTTG CTGTCAGTTT TTCGCTAAAG 
AGATTTCTAT TTTGCGAGTC CATTTATCTT CATGAAGTTA TAGAGGATTA TACCAATTTC 
CTGGCATCTT CCCCATTGGC AAGTGGACAA TCTGAAGGTC CAAGATGCCC AAAGGTTCTT 
GGTGACTTGG TAGAATCCTG TTTGGGGGCT CTTTTCCTCG ATTGTGGGTT CAACTTGAAT 
CATGTCTGGA CTATGATGCT ATCATTTCTA GATCCGGTCA AAAACTTGTC TAACCTTCAG 
ATTAGTCCTA TAAAAGAACT GATTGAACTT TGCCAGTCTT ACAAGTGGGA TCGGGAAATA 
TCAGCGACGA AAAAGGATGG TGCTTTTACT GTTGAACTAA AAGTGACCAA GAATGGTTGT 
TGCCTTACAG TTTCTGCAAC TGGTCGGAAC AAAAGAGAGG GCACAAAAAA GGCTGCACAG 
CTGATGATTA CAAACCTGAA GGCTCATGAG AACATAACAA CCTCCCATCC GTTGGAGGAT 
GTTCTGAAGA ATGGCATCCG AAATGAAGCT AAATTAATTG GCTACAATGA AGATCCTATA 
GATGTTGTGG ATCTTGTTGG GCTGGACGTT GAAAACCTAA ATATCCTAGA AACTTTTGGC 
GGGAATAGTG AAAGAAGCAG CTCATACGTC ATCAGACGAG GTCTCCCCCA AGCACCATCT 
AAAACAGAAG ACAGGCTTCC TCAAAAGGCC ATCATAAAAG CAGGTGGACC AAGCAGCAAA 
ACCGCAAAAT CCCTCTTGCA CGAAACATGT GTTGCTAACT GTTGGAAGCC ACCACACTTC 
GAATGTTGTG AAGAGGAAGG ACCAGGCCAC CTGAAATCAT TCGTCTACAA GGTAATCCTG 
GAAGTTGAAG ATGCGCCCAA TATGACATTG GAATGTTATG GTGAGGCTAG AGCAACGAAG 
AAAGGTGCAG CAGAGCACGC TGCCCAAGCT GCTATATGGT GCCTCAAGCA TTCTGGATTC 
CTTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCL4 ( NM_001203419.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190348.1 Arabidopsis thaliana dicer-like 4 (DCL4), mRNA.

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CloneID	OAb32357
Clone ID Related Accession (Same CDS sequence)	NM_001203419.2
Accession Version	NM_001203419.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	dicer-like 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_001203419.1.

ATGCGTGACG AAGTTGACTT GAGCTTGACC ATTCCCTCGA AGCTTTTGGG GAAGCGAGAC 
AGAGAACAAA AAAATTGTGA AGAAGAAAAA AACAAAAACA AAAAAGCTAA AAAGCAGCAA 
AAGGACCCAA TTCTTCTTCA CACTAGTGCT GCCACTCACA AGTTTCTTCC TCCTCCTTTG 
ACCATGCCGT ACAGTGAAAT CGGCGACGAT CTTCGCTCAC TCGACTTTGA CCACGCCGAT 
GTTTCTTCCG ACCTTCACCT CACTTCTTCT TCCTCTGTTT CTTCGTTTTC CTCTTCTTCG 
TCTTCTTTGT TCTCCGCGGC TGGTACGGAT GATCCTTCAC CGAAAATGGA GAAAGACCCT 
AGAAAAATCG CCAGGAGGTA TCAGGTGGAG CTGTGTAAGA AAGCAACGGA GGAGAACGTT 
ATTGTATATT TGGGTACAGG TTGTGGGAAG ACTCACATTG CAGTGATGCT TATATATGAG 
CTTGGTCATT TGGTTCTTAG TCCCAAGAAA AGTGTTTGTA TTTTTCTTGC TCCCACCGTG 
GCTTTGGTCG AACAGCAAGC CAAGGTCATA GCGGACTCTG TCAACTTCAA AGTTGCAATA 
CATTGTGGAG GCAAGAGGAT TGTGAAGAGC CACTCGGAGT GGGAGAGAGA GATTGCAGCG 
AATGAGCACT GTTTCATCAA GATGGAGTGT ATCTCCCTTC TAATATTTGA TGAGTGTCAC 
CATGCTCAAC AACAAAGCAA CCATCCTTAT GCAGAAATCA TGAAGGTTTT CTATAAATCG 
GAAAGTTTAC AACGGCCTCG AATATTTGGA ATGACTGCAT CTCCAGTTGT TGGCAAAGGG 
TCTTTTCAAT CAGAGAATTT ATCGAAAAGC ATTAATAGCC TTGAAAATTT GCTCAATGCC 
AAGGTTTATT CAGTGGAAAG CAATGTCCAG CTGGATGGTT TTGTTTCATC TCCTTTAGTC 
AAAGTATATT ATTATCGGTC AGCTTTAAGT GATGCATCTC AATCGACCAT CAGATATGAA 
AACATGCTGG AGGACATCAA ACAGCGGTGC TTGGCATCAC TTAAGCTGCT GATTGATACT 
CATCAAACAC AAACCCTCCT AAGTATGAAA AGGCTTCTCA AAAGATCTCA TGATAATCTC 
ATATATACTC TGCTGAATCT TGGCCTCTGG GGAGCAATAC AGGCTGCTAA AATCCAATTG 
AATAGTGACC ATAATGTACA AGACGAGCCT GTGGGAAAGA ATCCTAAGTC AAAGATATGT 
GATACATATC TTTCTATGGC TGCTGAGGCC CTCTCTTCTG GTGTTGCTAA AGATGAGAAT 
GCATCTGACC TCCTCAGCTT AGCGGCGTTG AAGGAACCAT TATTCTCTAG AAAGCTAGTT 
CAATTGATTA AGATCCTTTC GGTATTCAGG CTAGAGCCAC ACATGAAATG TATAATATTT 
GTCAATCGGA TTGTGACTGC AAGAACATTG TCATGCATAC TAAATAACTT GGAACTGCTA 
CGGTCTTGGA AGTCTGATTT CCTTGTTGGA CTTAGTTCTG GACTGAAGAG CATGTCAAGA 
AGGAGTATGG AAACAATACT TAAACGGTTC CAATCTAAAG AGCTCAATTT ACTGGTTGCC 
ACTAAAGTTG GTGAAGAAGG CCTTGATATT CAGACATGCT GTCTTGTGAT CCGTTATGAT 
TTACCAGAGA CTGTTACCAG CTTCATACAG TCCAGAGGTC GTGCTCGAAT GCCTCAGTCT 
GAATATGCGT TTCTAGTGGA CAGCGGAAAC GAGAAAGAGA TGGATCTTAT TGAAAATTTT 
AAAGTAAATG AAGATCGAAT GAATCTAGAA ATTACTTACA GAAGCTCAGA GGAAACTTGT 
CCTAGACTTG ATGAGGAGTT ATACAAAGTT CATGAGACAG GAGCTTGTAT CAGTGGTGGA 
AGCAGCATCT CCCTTCTCTA TAAATATTGT TCTAGGCTTC CACATGATGA ATTTTTTCAG 
CCCAAGCCAG AGTTTCAATT CAAGCCTGTT GACGAATTTG GTGGAACTAT CTGTCGCATA 
ACTTTACCTG CTAATGCTCC TATAAGTGAA ATCGAAAGTT CACTACTACC TTCGACAGAA 
GCTGCTAAAA AGGATGCTTG TCTAAAGGCT GTGCATGAGT TGCACAACTT GGGTGTACTT 
AACGATTTTC TGTTGCCAGA TTCCAAGGAT GAAATTGAGG ACGAATTGTC AGATGATGAA 
TTTGATTTTG ATAACATCAA AGGTGAAGGC TGTTCACGAG GTGACCTGTA TGAGATGCGT 
GTACCAGTCT TGTTTAAACA AAAGTGGGAT CCATCTACAA GTTGTGTCAA TCTTCATTCT 
TACTATATAA TGTTTGTGCC TCATCCCGCT GATAGGATCT ACAAAAAGTT TGGTTTCTTC 
ATGAAGTCAC CTCTTCCCGT TGAGGCTGAG ACTATGGATA TCGATCTTCA CCTTGCTCAT 
CAAAGATCTG TAAGTGTAAA GATTTTTCCA TCAGGGGTCA CAGAATTCGA CAACGATGAG 
ATAAGACTAG CTGAGCTTTT CCAGGAGATT GCCCTGAAGG TTCTTTTTGA ACGGGGGGAG 
CTGATCCCGG ACTTTGTTCC CTTGGAACTG CAAGACTCTT CTAGAACAAG CAAATCCACC 
TTCTACCTTC TTCTTCCACT CTGTCTGCAT GATGGAGAAA GTGTTATATC TGTAGATTGG 
GTGACTATCA GAAACTGCTT GTCATCACCA ATCTTTAAGA CTCCATCTGT TTTAGTGGAA 
GATATATTTC CTCCTTCGGG CTCTCATTTA AAGCTAGCAA ATGGCTGCTG GAATATTGAT 
GATGTGAAGA ACAGCTTGGT TTTTACAACC TACAGTAAAC AATTTTACTT TGTTGCTGAT 
ATCTGCCATG GAAGAAATGG TTTCAGTCCT GTTAAGGAAT CTAGCACCAA AAGCCATGTG 
GAGAGCATAT ATAAGTTGTA TGGCGTGGAA CTCAAGCATC CTGCACAGCC ACTCTTGCGT 
GTGAAACCAC TTTGTCATGT TCGGAACTTG CTTCACAACC GAATGCAGAC GAATTTGGAA 
CCACAAGAAC TTGACGAATA CTTCATAGAG ATTCCTCCCG AACTTTCTCA CTTAAAGATA 
AAAGGATTAT CTAAAGACAT CGGAAGCTCG TTATCCTTGT TACCATCAAT CATGCATCGT 
ATGGAGAATT TACTCGTGGC TATTGAACTG AAACATGTGC TGTCTGCTTC GATCCCTGAG 
ATAGCTGAAG TTTCTGGTCA CAGGGTACTC GAGGCGCTCA CAACAGAGAA ATGTCATGAG 
CGCCTTTCTC TTGAAAGGCT TGAGGTGCTT GGTGATGCAT TCCTCAAGTT TGCTGTTAGC 
CGACACCTTT TTCTACACCA TGATAGTCTT GATGAAGGAG AGTTGACTCG GAGACGCTCT 
AACGTTGTTA ACAATTCCAA CTTGTGCAGG CTTGCAATAA AAAAAAATCT GCAGGTCTAC 
ATCCGTGATC AAGCATTGGA TCCTACTCAG TTCTTTGCAT TTGGCCATCC ATGCAGAGTA 
ACCTGTGACG AGGTAGCCAG TAAAGAGGTT CATTCCTTGA ATAGGGATCT TGGGATCTTG 
GAGTCAAATA CTGGTGAAAT CAGATGTAGC AAAGGCCATC ATTGGTTGTA CAAGAAAACA 
ATTGCTGATG TGGTTGAGGC TCTTGTGGGA GCTTTCTTAG TTGACAGTGG CTTCAAAGGT 
GCTGTGAAAT TTCTGAAGTG GATTGGTGTA AATGTTGATT TTGAATCCTT GCAAGTACAA 
GATGCTTGTA TTGCAAGCAG GCGCTACTTG CCCCTCACTA CTCGCAATAA TTTGGAGACC 
CTTGAAAACC AGCTTGACTA TAAGTTCCTC CACAAAGGTC TACTTGTACA AGCCTTTATC 
CATCCATCTT ACAACAGGCA TGGAGGAGGC TGCTACCAGA GATTGGAGTT TCTTGGGGAT 
GCTGTTCTGG ACTACTTGAT GACATCCTAT TTTTTCACAG TCTTCCCGAA ACTGAAACCT 
GGTCAACTGA CCGATCTAAG ATCTCTCTCA GTAAATAATG AGGCGCTAGC AAATGTTGCT 
GTCAGTTTTT CGCTAAAGAG ATTTCTATTT TGCGAGTCCA TTTATCTTCA TGAAGTTATA 
GAGGATTATA CCAATTTCCT GGCATCTTCC CCATTGGCAA GTGGACAATC TGAAGGTCCA 
AGATGCCCAA AGGTTCTTGG TGACTTGGTA GAATCCTGTT TGGGGGCTCT TTTCCTCGAT 
TGTGGGTTCA ACTTGAATCA TGTCTGGACT ATGATGCTAT CATTTCTAGA TCCGGTCAAA 
AACTTGTCTA ACCTTCAGAT TAGTCCTATA AAAGAACTGA TTGAACTTTG CCAGTCTTAC 
AAGTGGGATC GGGAAATATC AGCGACGAAA AAGGATGGTG CTTTTACTGT TGAACTAAAA 
GTGACCAAGA ATGGTTGTTG CCTTACAGTT TCTGCAACTG GTCGGAACAA AAGAGAGGGC 
ACAAAAAAGG CTGCACAGCT GATGATTACA AACCTGAAGG CTCATGAGAA CATAACAACC 
TCCCATCCGT TGGAGGATGT TCTGAAGAAT GGCATCCGAA ATGAAGCTAA ATTAATTGGC 
TACAATGAAG ATCCTATAGA TGTTGTGGAT CTTGTTGGGC TGGACGTTGA AAACCTAAAT 
ATCCTAGAAA CTTTTGGCGG GAATAGTGAA AGAAGCAGCT CATACGTCAT CAGACGAGGT 
CTCCCCCAAG CACCATCTAA AACAGAAGAC AGGCTTCCTC AAAAGGCCAT CATAAAAGCA 
GGTGGACCAA GCAGCAAAAC CGCAAAATCC CTCTTGCACG AAACATGTGT TGCTAACTGT 
TGGAAGCCAC CACACTTCGA ATGTTGTGAA GAGGAAGGAC CAGGCCACCT GAAATCATTC 
GTCTACAAGG TAATCCTGGA AGTTGAAGAT GCGCCCAATA TGACATTGGA ATGTTATGGT 
GAGGCTAGAG CAACGAAGAA AGGTGCAGCA GAGCACGCTG CCCAAGCTGC TATATGGTGC 
CTCAAGCATT CTGGATTCCT TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190348.1
CDS	240..5306
Translation	MRDEVDLSLTIPSKLLGKRDREQKNCEEEKNKNKKAKKQQKDPILLHTSAATHKFLPPPLTMPYSEIGDDLRSLDFDHADVSSDLHLTSSSSVSSFSSSSSSLFSAAGTDDPSPKMEKDPRKIARRYQVELCKKATEENVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKVAIHCGGKRIVKSHSEWEREIAANEHCFIKMECISLLIFDECHHAQQQSNHPYAEIMKVFYKSESLQRPRIFGMTASPVVGKGSFQSENLSKSINSLENLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENMLEDIKQRCLASLKLLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNLGLWGAIQAAKIQLNSDHNVQDEPVGKNPKSKICDTYLSMAAEALSSGVAKDENASDLLSLAALKEPLFSRKLVQLIKILSVFRLEPHMKCIIFVNRIVTARTLSCILNNLELLRSWKSDFLVGLSSGLKSMSRRSMETILKRFQSKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQSEYAFLVDSGNEKEMDLIENFKVNEDRMNLEITYRSSEETCPRLDEELYKVHETGACISGGSSISLLYKYCSRLPHDEFFQPKPEFQFKPVDEFGGTICRITLPANAPISEIESSLLPSTEAAKKDACLKAVHELHNLGVLNDFLLPDSKDEIEDELSDDEFDFDNIKGEGCSRGDLYEMRVPVLFKQKWDPSTSCVNLHSYYIMFVPHPADRIYKKFGFFMKSPLPVEAETMDIDLHLAHQRSVSVKIFPSGVTEFDNDEIRLAELFQEIALKVLFERGELIPDFVPLELQDSSRTSKSTFYLLLPLCLHDGESVISVDWVTIRNCLSSPIFKTPSVLVEDIFPPSGSHLKLANGCWNIDDVKNSLVFTTYSKQFYFVADICHGRNGFSPVKESSTKSHVESIYKLYGVELKHPAQPLLRVKPLCHVRNLLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIELKHVLSASIPEIAEVSGHRVLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLFLHHDSLDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQALDPTQFFAFGHPCRVTCDEVASKEVHSLNRDLGILESNTGEIRCSKGHHWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKWIGVNVDFESLQVQDACIASRRYLPLTTRNNLETLENQLDYKFLHKGLLVQAFIHPSYNRHGGGCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQLTDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVIEDYTNFLASSPLASGQSEGPRCPKVLGDLVESCLGALFLDCGFNLNHVWTMMLSFLDPVKNLSNLQISPIKELIELCQSYKWDREISATKKDGAFTVELKVTKNGCCLTVSATGRNKREGTKKAAQLMITNLKAHENITTSHPLEDVLKNGIRNEAKLIGYNEDPIDVVDLVGLDVENLNILETFGGNSERSSSYVIRRGLPQAPSKTEDRLPQKAIIKAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLECYGEARATKKGAAEHAAQAAIWCLKHSGFLC

Target ORF information:

RefSeq Version	NM_001203419.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana dicer-like 4 (DCL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203419.2

ORF Insert Sequence:

ATGCGTGACG AAGTTGACTT GAGCTTGACC ATTCCCTCGA AGCTTTTGGG GAAGCGAGAC 
AGAGAACAAA AAAATTGTGA AGAAGAAAAA AACAAAAACA AAAAAGCTAA AAAGCAGCAA 
AAGGACCCAA TTCTTCTTCA CACTAGTGCT GCCACTCACA AGTTTCTTCC TCCTCCTTTG 
ACCATGCCGT ACAGTGAAAT CGGCGACGAT CTTCGCTCAC TCGACTTTGA CCACGCCGAT 
GTTTCTTCCG ACCTTCACCT CACTTCTTCT TCCTCTGTTT CTTCGTTTTC CTCTTCTTCG 
TCTTCTTTGT TCTCCGCGGC TGGTACGGAT GATCCTTCAC CGAAAATGGA GAAAGACCCT 
AGAAAAATCG CCAGGAGGTA TCAGGTGGAG CTGTGTAAGA AAGCAACGGA GGAGAACGTT 
ATTGTATATT TGGGTACAGG TTGTGGGAAG ACTCACATTG CAGTGATGCT TATATATGAG 
CTTGGTCATT TGGTTCTTAG TCCCAAGAAA AGTGTTTGTA TTTTTCTTGC TCCCACCGTG 
GCTTTGGTCG AACAGCAAGC CAAGGTCATA GCGGACTCTG TCAACTTCAA AGTTGCAATA 
CATTGTGGAG GCAAGAGGAT TGTGAAGAGC CACTCGGAGT GGGAGAGAGA GATTGCAGCG 
AATGAGCACT GTTTCATCAA GATGGAGTGT ATCTCCCTTC TAATATTTGA TGAGTGTCAC 
CATGCTCAAC AACAAAGCAA CCATCCTTAT GCAGAAATCA TGAAGGTTTT CTATAAATCG 
GAAAGTTTAC AACGGCCTCG AATATTTGGA ATGACTGCAT CTCCAGTTGT TGGCAAAGGG 
TCTTTTCAAT CAGAGAATTT ATCGAAAAGC ATTAATAGCC TTGAAAATTT GCTCAATGCC 
AAGGTTTATT CAGTGGAAAG CAATGTCCAG CTGGATGGTT TTGTTTCATC TCCTTTAGTC 
AAAGTATATT ATTATCGGTC AGCTTTAAGT GATGCATCTC AATCGACCAT CAGATATGAA 
AACATGCTGG AGGACATCAA ACAGCGGTGC TTGGCATCAC TTAAGCTGCT GATTGATACT 
CATCAAACAC AAACCCTCCT AAGTATGAAA AGGCTTCTCA AAAGATCTCA TGATAATCTC 
ATATATACTC TGCTGAATCT TGGCCTCTGG GGAGCAATAC AGGCTGCTAA AATCCAATTG 
AATAGTGACC ATAATGTACA AGACGAGCCT GTGGGAAAGA ATCCTAAGTC AAAGATATGT 
GATACATATC TTTCTATGGC TGCTGAGGCC CTCTCTTCTG GTGTTGCTAA AGATGAGAAT 
GCATCTGACC TCCTCAGCTT AGCGGCGTTG AAGGAACCAT TATTCTCTAG AAAGCTAGTT 
CAATTGATTA AGATCCTTTC GGTATTCAGG CTAGAGCCAC ACATGAAATG TATAATATTT 
GTCAATCGGA TTGTGACTGC AAGAACATTG TCATGCATAC TAAATAACTT GGAACTGCTA 
CGGTCTTGGA AGTCTGATTT CCTTGTTGGA CTTAGTTCTG GACTGAAGAG CATGTCAAGA 
AGGAGTATGG AAACAATACT TAAACGGTTC CAATCTAAAG AGCTCAATTT ACTGGTTGCC 
ACTAAAGTTG GTGAAGAAGG CCTTGATATT CAGACATGCT GTCTTGTGAT CCGTTATGAT 
TTACCAGAGA CTGTTACCAG CTTCATACAG TCCAGAGGTC GTGCTCGAAT GCCTCAGTCT 
GAATATGCGT TTCTAGTGGA CAGCGGAAAC GAGAAAGAGA TGGATCTTAT TGAAAATTTT 
AAAGTAAATG AAGATCGAAT GAATCTAGAA ATTACTTACA GAAGCTCAGA GGAAACTTGT 
CCTAGACTTG ATGAGGAGTT ATACAAAGTT CATGAGACAG GAGCTTGTAT CAGTGGTGGA 
AGCAGCATCT CCCTTCTCTA TAAATATTGT TCTAGGCTTC CACATGATGA ATTTTTTCAG 
CCCAAGCCAG AGTTTCAATT CAAGCCTGTT GACGAATTTG GTGGAACTAT CTGTCGCATA 
ACTTTACCTG CTAATGCTCC TATAAGTGAA ATCGAAAGTT CACTACTACC TTCGACAGAA 
GCTGCTAAAA AGGATGCTTG TCTAAAGGCT GTGCATGAGT TGCACAACTT GGGTGTACTT 
AACGATTTTC TGTTGCCAGA TTCCAAGGAT GAAATTGAGG ACGAATTGTC AGATGATGAA 
TTTGATTTTG ATAACATCAA AGGTGAAGGC TGTTCACGAG GTGACCTGTA TGAGATGCGT 
GTACCAGTCT TGTTTAAACA AAAGTGGGAT CCATCTACAA GTTGTGTCAA TCTTCATTCT 
TACTATATAA TGTTTGTGCC TCATCCCGCT GATAGGATCT ACAAAAAGTT TGGTTTCTTC 
ATGAAGTCAC CTCTTCCCGT TGAGGCTGAG ACTATGGATA TCGATCTTCA CCTTGCTCAT 
CAAAGATCTG TAAGTGTAAA GATTTTTCCA TCAGGGGTCA CAGAATTCGA CAACGATGAG 
ATAAGACTAG CTGAGCTTTT CCAGGAGATT GCCCTGAAGG TTCTTTTTGA ACGGGGGGAG 
CTGATCCCGG ACTTTGTTCC CTTGGAACTG CAAGACTCTT CTAGAACAAG CAAATCCACC 
TTCTACCTTC TTCTTCCACT CTGTCTGCAT GATGGAGAAA GTGTTATATC TGTAGATTGG 
GTGACTATCA GAAACTGCTT GTCATCACCA ATCTTTAAGA CTCCATCTGT TTTAGTGGAA 
GATATATTTC CTCCTTCGGG CTCTCATTTA AAGCTAGCAA ATGGCTGCTG GAATATTGAT 
GATGTGAAGA ACAGCTTGGT TTTTACAACC TACAGTAAAC AATTTTACTT TGTTGCTGAT 
ATCTGCCATG GAAGAAATGG TTTCAGTCCT GTTAAGGAAT CTAGCACCAA AAGCCATGTG 
GAGAGCATAT ATAAGTTGTA TGGCGTGGAA CTCAAGCATC CTGCACAGCC ACTCTTGCGT 
GTGAAACCAC TTTGTCATGT TCGGAACTTG CTTCACAACC GAATGCAGAC GAATTTGGAA 
CCACAAGAAC TTGACGAATA CTTCATAGAG ATTCCTCCCG AACTTTCTCA CTTAAAGATA 
AAAGGATTAT CTAAAGACAT CGGAAGCTCG TTATCCTTGT TACCATCAAT CATGCATCGT 
ATGGAGAATT TACTCGTGGC TATTGAACTG AAACATGTGC TGTCTGCTTC GATCCCTGAG 
ATAGCTGAAG TTTCTGGTCA CAGGGTACTC GAGGCGCTCA CAACAGAGAA ATGTCATGAG 
CGCCTTTCTC TTGAAAGGCT TGAGGTGCTT GGTGATGCAT TCCTCAAGTT TGCTGTTAGC 
CGACACCTTT TTCTACACCA TGATAGTCTT GATGAAGGAG AGTTGACTCG GAGACGCTCT 
AACGTTGTTA ACAATTCCAA CTTGTGCAGG CTTGCAATAA AAAAAAATCT GCAGGTCTAC 
ATCCGTGATC AAGCATTGGA TCCTACTCAG TTCTTTGCAT TTGGCCATCC ATGCAGAGTA 
ACCTGTGACG AGGTAGCCAG TAAAGAGGTT CATTCCTTGA ATAGGGATCT TGGGATCTTG 
GAGTCAAATA CTGGTGAAAT CAGATGTAGC AAAGGCCATC ATTGGTTGTA CAAGAAAACA 
ATTGCTGATG TGGTTGAGGC TCTTGTGGGA GCTTTCTTAG TTGACAGTGG CTTCAAAGGT 
GCTGTGAAAT TTCTGAAGTG GATTGGTGTA AATGTTGATT TTGAATCCTT GCAAGTACAA 
GATGCTTGTA TTGCAAGCAG GCGCTACTTG CCCCTCACTA CTCGCAATAA TTTGGAGACC 
CTTGAAAACC AGCTTGACTA TAAGTTCCTC CACAAAGGTC TACTTGTACA AGCCTTTATC 
CATCCATCTT ACAACAGGCA TGGAGGAGGC TGCTACCAGA GATTGGAGTT TCTTGGGGAT 
GCTGTTCTGG ACTACTTGAT GACATCCTAT TTTTTCACAG TCTTCCCGAA ACTGAAACCT 
GGTCAACTGA CCGATCTAAG ATCTCTCTCA GTAAATAATG AGGCGCTAGC AAATGTTGCT 
GTCAGTTTTT CGCTAAAGAG ATTTCTATTT TGCGAGTCCA TTTATCTTCA TGAAGTTATA 
GAGGATTATA CCAATTTCCT GGCATCTTCC CCATTGGCAA GTGGACAATC TGAAGGTCCA 
AGATGCCCAA AGGTTCTTGG TGACTTGGTA GAATCCTGTT TGGGGGCTCT TTTCCTCGAT 
TGTGGGTTCA ACTTGAATCA TGTCTGGACT ATGATGCTAT CATTTCTAGA TCCGGTCAAA 
AACTTGTCTA ACCTTCAGAT TAGTCCTATA AAAGAACTGA TTGAACTTTG CCAGTCTTAC 
AAGTGGGATC GGGAAATATC AGCGACGAAA AAGGATGGTG CTTTTACTGT TGAACTAAAA 
GTGACCAAGA ATGGTTGTTG CCTTACAGTT TCTGCAACTG GTCGGAACAA AAGAGAGGGC 
ACAAAAAAGG CTGCACAGCT GATGATTACA AACCTGAAGG CTCATGAGAA CATAACAACC 
TCCCATCCGT TGGAGGATGT TCTGAAGAAT GGCATCCGAA ATGAAGCTAA ATTAATTGGC 
TACAATGAAG ATCCTATAGA TGTTGTGGAT CTTGTTGGGC TGGACGTTGA AAACCTAAAT 
ATCCTAGAAA CTTTTGGCGG GAATAGTGAA AGAAGCAGCT CATACGTCAT CAGACGAGGT 
CTCCCCCAAG CACCATCTAA AACAGAAGAC AGGCTTCCTC AAAAGGCCAT CATAAAAGCA 
GGTGGACCAA GCAGCAAAAC CGCAAAATCC CTCTTGCACG AAACATGTGT TGCTAACTGT 
TGGAAGCCAC CACACTTCGA ATGTTGTGAA GAGGAAGGAC CAGGCCACCT GAAATCATTC 
GTCTACAAGG TAATCCTGGA AGTTGAAGAT GCGCCCAATA TGACATTGGA ATGTTATGGT 
GAGGCTAGAG CAACGAAGAA AGGTGCAGCA GAGCACGCTG CCCAAGCTGC TATATGGTGC 
CTCAAGCATT CTGGATTCCT TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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DCL4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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