HK5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	HK5
Entrez Gene ID	830938
Full Name	histidine kinase 5
Synonyms	AHK5,CKI2,CYTOKININ INDEPENDENT 2,MAJ23.80,MAJ23_80,histidine kinase 5
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_121110.2	NP_196633.2	histidine kinase 5

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0654300	NP_001058244.1
Arabidopsis thaliana (thale cress)	HK5	NP_196633.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The first crystal structure of a plant histidine kinase receiver domain(RD), AHK5(RD), in complex with one of its cognate phosphotransfer proteins, AHP1.
Title: Structure-function analysis of Arabidopsis thaliana histidine kinase AHK5 bound to its cognate phosphotransfer protein AHP1.

data suggest the presence of a complex TCS signaling network comprising of at least AHK5, several AHPs and response regulators, which modulate stomatal closure in response to HO and ethylene.
Title: Identification of two-component system elements downstream of AHK5 in the stomatal closure response of Arabidopsis thaliana.

A model which proposes a role for reactive oxygen species (ROS) and hormones in integrating abiotic and biotic stress responses via AHK5, is proposed.
Title: Modulation of ROS production and hormone levels by AHK5 during abiotic and biotic stress signaling.

This is the first report of a role for AHK5 in the regulation of survival following challenge by a hemi-biotrophic bacterium and a necrotrophic fungus, as well as in the growth response to salt stress.
Title: Arabidopsis histidine kinase 5 regulates salt sensitivity and resistance against bacterial and fungal infection.

findings identify an integral signalling function for AHK5 that acts to integrate multiple signals via H(2)O(2) homeostasis and is independent of ABA signalling in guard cells
Title: The histidine kinase AHK5 integrates endogenous and environmental signals in Arabidopsis guard cells.

The following HK5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HK5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb18125	NM_121110.2 Latest version!	Arabidopsis thaliana histidine kinase 5 (HK5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HK5 ( NM_121110.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196633.2 Arabidopsis thaliana histidine kinase 5 (HK5), mRNA.

Price & Availability↑

CloneID	OAb18125
Clone ID Related Accession (Same CDS sequence)	NM_121110.2
Accession Version	NM_121110.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	histidine kinase 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Apr 18, 2007 this sequence version replaced NM_121110.1.

ATGGTCTGTG AAATGGAGAC TGATCAGATT GAGGAAATGG ATGTCGAAGT TTTGTCTTCG 
ATGTGGCCCG AAGATGTTGG AACTGAAGCT GACAAACAGT TCAACGTCGA GAAACCTGCC 
GGAGATTTAG ACACGTTGAA AGAAGTTACT ATCGAGACAC GGACCATTGC GGATATGACA 
CGGTTACCAA ACCTATTGAA TTCGACTCAT CAAGGCTCCT CTCAACTAAC CAACCTTGTG 
AAACAATGGG AGTATATGCA AGACAACGCG GTTCGGCTGT TAAAAGAAGA GCTAAAAAAT 
CTCGATAGAC AGAGAGAAGA AGCCGAGGCT AAAGAGTTGA AGATCATTGA GGAGTATAAG 
TTTGAGAGCA ACGAGCCTGA GAATGTTCCG GTTTTGGATG AGACGAGTGA TTTGTTCCGC 
AGGTTTAGGC AGAAAAAACG AGATGCCTTG GTCGATAGCA AGAAGATTGA GATCTATGAG 
GAGTTTGACA CTGTTGCATA TTGGAAACAG AAGGCGTTGA GTCTTGAGAA AATGCTTGAG 
GCGAGTACTG AGAGAGAAAG GCGATTGATG GAGAAGCTGA GTGAGAGTTT GAAAACTATG 
GAGAGTCAGT CAGCACCGGT CCAAGAGCTT ACTCAGAATC TTAAGAGAGC TGAAGGTTTC 
TTGCATTTCA TACTTCAGAA TGCACCTATT GTTATGGGCC ATCAGGATAA AGATTTACGC 
TACTTGTTCA TCTACAACAA GTATCCTAGT TTACGGGAAC AGGACATTTT GGGAAAAACA 
GACGTGGAGA TATTCCATGG AGGTGGAGTT AAAGAATCTG AAGATTTCAA GAGAGAAGTT 
CTTGAGAAAG GAAAAGCTTC AAAGAGAGAG ATCACATTTA CTACAGATTT ATTTGGATCA 
AAGACGTTTT TGATATATGT TGAGCCTGTT TACAACAAAG CTGGCGAGAA AATCGGTATA 
AACTACATGG GAATGGAAGT AACTGATCAG GTAGTGAAAA GGGAGAAAAT GGCGAAACTT 
AGAGAAGATA ACGCTGTGAG AAAGGCGATG GAATCAGAAC TGAACAAGAC TATTCACATT 
ACAGAGGAGA CAATGAGAGC AAAGCAGATG CTAGCGACGA TGTCTCATGA GATAAGGTCA 
CCATTGTCAG GAGTAGTGGG AATGGCTGAG ATACTTTCAA CTACAAAACT GGATAAAGAG 
CAAAGACAGT TGTTGAATGT CATGATCTCT TCTGGTGATT TGGTGCTTCA GCTAATCAAC 
GACATTCTTG ATCTCTCCAA GGTTGAATCA GGTGTGATGA GATTAGAAGC TACAAAGTTT 
CGACCAAGAG AAGTAGTGAA GCATGTGCTA CAGACAGCTG CAGCATCGCT GAAGAAATCT 
TTGACATTAG AAGGAAACAT TGCAGATGAT GTTCCTATTG AGGTAGTTGG AGATGTTCTA 
AGGATTAGGC AGATCCTCAC CAATTTGATA AGCAATGCTA TCAAGTTTAC ACATGAAGGA 
AATGTAGGAA TCAAACTCCA AGTGATATCA GAACCATCCT TTGTGCGGGA TAACGCATTG 
AACGCAGACA CCGAGGAACA CGAACAAAAC GGTTTGACCG AGACTTCAGT TTGGATTTGC 
TGTGACGTAT GGGACACTGG AATTGGAATC CCAGAAAACG CTCTTCCATG TTTGTTCAAG 
AAGTACATGC AAGCAAGCGC TGATCATGCC CGAAAATACG GTGGGACTGG TCTCGGACTT 
GCTATTTGTA AACAGCTGGT TGAGTTAATG GGAGGCCAAC TCACTGTGAC AAGCCGGGTG 
AGCGAAGGTT CAACGTTCAC ATTTATATTA CCCTACAAAG TTGGAAGATC AGATGATTAT 
TCAGATGATC AAGATGAGTT CTCTGATATG GCGGATCAAC AATCTGAACC AGACGATACA 
GCTGAAGGAT ATTTCCAGTT TAAACCGCTC TTAGGATCGA TATATTCGAA TGGCGGACCG 
GGGATCAGCA ATGACTTCTT ACCTCATAAA GTCATGCTTA CTAGTCCTAT TAAGCTCATC 
AATGGTTTTG TCGCTGATCC CTCTAATAAC ACTGGACAGA GCGAGATGCT ACAGCTTGAA 
AACGGTGGTT ACATGGATGA ATCTAAACTC GAAACCAGTT CTGGTCATTG CCCTGAATCA 
GCTCACCAAT ATGAGAATGG AAATGGTCGA TGTTTCTCTA AGGAATCTGA ATCTTGTAGC 
AGTTCACAAG CTAGCTCAGA AGGTGGAACC TTAGAAATGG AGTCAGAGCT CACAGTTTCA 
TCTCATAGGG AAGAGGAAAA AGCCGAGACA GAAGTAAAAG AAACATCAAA GCCAAAGATT 
TTGCTTGTGG AAGATAATAA GATCAACATC ATGGTTGCAA AGTCGATGAT GAAGCAGTTA 
GGCCATACCA TGGATATTGC TAATAATGGA GTTGAAGCCA TAACCGCGAT TAATAGCTCT 
AGCTACGATC TGGTACTCAT GGATGTGTGC ATGCCGGTGC TCGATGGTTT AAAAGCTACA 
AGACTGATCC GTTCGTATGA AGAAACTGGG AACTGGAATG CTGCAATAGA AGCCGGAGTA 
GATATATCGA CATCGGAGAA TGAACAAGTT TGTATGCGTC CCACAAACCG GCTGCCTATA 
ATCGCGATGA CGGCAAATAC TTTAGCAGAG AGTTCAGAAG AATGTTATGC AAATGGTATG 
GACTCGTTTA TTTCGAAACC TGTAACGTTG CAAAAACTGA GAGAGTGTTT GCAACAGTAT 
TTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196633.2
CDS	378..3146
Translation	MVCEMETDQIEEMDVEVLSSMWPEDVGTEADKQFNVEKPAGDLDTLKEVTIETRTIADMTRLPNLLNSTHQGSSQLTNLVKQWEYMQDNAVRLLKEELKNLDRQREEAEAKELKIIEEYKFESNEPENVPVLDETSDLFRRFRQKKRDALVDSKKIEIYEEFDTVAYWKQKALSLEKMLEASTERERRLMEKLSESLKTMESQSAPVQELTQNLKRAEGFLHFILQNAPIVMGHQDKDLRYLFIYNKYPSLREQDILGKTDVEIFHGGGVKESEDFKREVLEKGKASKREITFTTDLFGSKTFLIYVEPVYNKAGEKIGINYMGMEVTDQVVKREKMAKLREDNAVRKAMESELNKTIHITEETMRAKQMLATMSHEIRSPLSGVVGMAEILSTTKLDKEQRQLLNVMISSGDLVLQLINDILDLSKVESGVMRLEATKFRPREVVKHVLQTAAASLKKSLTLEGNIADDVPIEVVGDVLRIRQILTNLISNAIKFTHEGNVGIKLQVISEPSFVRDNALNADTEEHEQNGLTETSVWICCDVWDTGIGIPENALPCLFKKYMQASADHARKYGGTGLGLAICKQLVELMGGQLTVTSRVSEGSTFTFILPYKVGRSDDYSDDQDEFSDMADQQSEPDDTAEGYFQFKPLLGSIYSNGGPGISNDFLPHKVMLTSPIKLINGFVADPSNNTGQSEMLQLENGGYMDESKLETSSGHCPESAHQYENGNGRCFSKESESCSSSQASSEGGTLEMESELTVSSHREEEKAETEVKETSKPKILLVEDNKINIMVAKSMMKQLGHTMDIANNGVEAITAINSSSYDLVLMDVCMPVLDGLKATRLIRSYEETGNWNAAIEAGVDISTSENEQVCMRPTNRLPIIAMTANTLAESSEECYANGMDSFISKPVTLQKLRECLQQYLH

Target ORF information:

RefSeq Version	NM_121110.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana histidine kinase 5 (HK5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121110.2

ORF Insert Sequence:

ATGGTCTGTG AAATGGAGAC TGATCAGATT GAGGAAATGG ATGTCGAAGT TTTGTCTTCG 
ATGTGGCCCG AAGATGTTGG AACTGAAGCT GACAAACAGT TCAACGTCGA GAAACCTGCC 
GGAGATTTAG ACACGTTGAA AGAAGTTACT ATCGAGACAC GGACCATTGC GGATATGACA 
CGGTTACCAA ACCTATTGAA TTCGACTCAT CAAGGCTCCT CTCAACTAAC CAACCTTGTG 
AAACAATGGG AGTATATGCA AGACAACGCG GTTCGGCTGT TAAAAGAAGA GCTAAAAAAT 
CTCGATAGAC AGAGAGAAGA AGCCGAGGCT AAAGAGTTGA AGATCATTGA GGAGTATAAG 
TTTGAGAGCA ACGAGCCTGA GAATGTTCCG GTTTTGGATG AGACGAGTGA TTTGTTCCGC 
AGGTTTAGGC AGAAAAAACG AGATGCCTTG GTCGATAGCA AGAAGATTGA GATCTATGAG 
GAGTTTGACA CTGTTGCATA TTGGAAACAG AAGGCGTTGA GTCTTGAGAA AATGCTTGAG 
GCGAGTACTG AGAGAGAAAG GCGATTGATG GAGAAGCTGA GTGAGAGTTT GAAAACTATG 
GAGAGTCAGT CAGCACCGGT CCAAGAGCTT ACTCAGAATC TTAAGAGAGC TGAAGGTTTC 
TTGCATTTCA TACTTCAGAA TGCACCTATT GTTATGGGCC ATCAGGATAA AGATTTACGC 
TACTTGTTCA TCTACAACAA GTATCCTAGT TTACGGGAAC AGGACATTTT GGGAAAAACA 
GACGTGGAGA TATTCCATGG AGGTGGAGTT AAAGAATCTG AAGATTTCAA GAGAGAAGTT 
CTTGAGAAAG GAAAAGCTTC AAAGAGAGAG ATCACATTTA CTACAGATTT ATTTGGATCA 
AAGACGTTTT TGATATATGT TGAGCCTGTT TACAACAAAG CTGGCGAGAA AATCGGTATA 
AACTACATGG GAATGGAAGT AACTGATCAG GTAGTGAAAA GGGAGAAAAT GGCGAAACTT 
AGAGAAGATA ACGCTGTGAG AAAGGCGATG GAATCAGAAC TGAACAAGAC TATTCACATT 
ACAGAGGAGA CAATGAGAGC AAAGCAGATG CTAGCGACGA TGTCTCATGA GATAAGGTCA 
CCATTGTCAG GAGTAGTGGG AATGGCTGAG ATACTTTCAA CTACAAAACT GGATAAAGAG 
CAAAGACAGT TGTTGAATGT CATGATCTCT TCTGGTGATT TGGTGCTTCA GCTAATCAAC 
GACATTCTTG ATCTCTCCAA GGTTGAATCA GGTGTGATGA GATTAGAAGC TACAAAGTTT 
CGACCAAGAG AAGTAGTGAA GCATGTGCTA CAGACAGCTG CAGCATCGCT GAAGAAATCT 
TTGACATTAG AAGGAAACAT TGCAGATGAT GTTCCTATTG AGGTAGTTGG AGATGTTCTA 
AGGATTAGGC AGATCCTCAC CAATTTGATA AGCAATGCTA TCAAGTTTAC ACATGAAGGA 
AATGTAGGAA TCAAACTCCA AGTGATATCA GAACCATCCT TTGTGCGGGA TAACGCATTG 
AACGCAGACA CCGAGGAACA CGAACAAAAC GGTTTGACCG AGACTTCAGT TTGGATTTGC 
TGTGACGTAT GGGACACTGG AATTGGAATC CCAGAAAACG CTCTTCCATG TTTGTTCAAG 
AAGTACATGC AAGCAAGCGC TGATCATGCC CGAAAATACG GTGGGACTGG TCTCGGACTT 
GCTATTTGTA AACAGCTGGT TGAGTTAATG GGAGGCCAAC TCACTGTGAC AAGCCGGGTG 
AGCGAAGGTT CAACGTTCAC ATTTATATTA CCCTACAAAG TTGGAAGATC AGATGATTAT 
TCAGATGATC AAGATGAGTT CTCTGATATG GCGGATCAAC AATCTGAACC AGACGATACA 
GCTGAAGGAT ATTTCCAGTT TAAACCGCTC TTAGGATCGA TATATTCGAA TGGCGGACCG 
GGGATCAGCA ATGACTTCTT ACCTCATAAA GTCATGCTTA CTAGTCCTAT TAAGCTCATC 
AATGGTTTTG TCGCTGATCC CTCTAATAAC ACTGGACAGA GCGAGATGCT ACAGCTTGAA 
AACGGTGGTT ACATGGATGA ATCTAAACTC GAAACCAGTT CTGGTCATTG CCCTGAATCA 
GCTCACCAAT ATGAGAATGG AAATGGTCGA TGTTTCTCTA AGGAATCTGA ATCTTGTAGC 
AGTTCACAAG CTAGCTCAGA AGGTGGAACC TTAGAAATGG AGTCAGAGCT CACAGTTTCA 
TCTCATAGGG AAGAGGAAAA AGCCGAGACA GAAGTAAAAG AAACATCAAA GCCAAAGATT 
TTGCTTGTGG AAGATAATAA GATCAACATC ATGGTTGCAA AGTCGATGAT GAAGCAGTTA 
GGCCATACCA TGGATATTGC TAATAATGGA GTTGAAGCCA TAACCGCGAT TAATAGCTCT 
AGCTACGATC TGGTACTCAT GGATGTGTGC ATGCCGGTGC TCGATGGTTT AAAAGCTACA 
AGACTGATCC GTTCGTATGA AGAAACTGGG AACTGGAATG CTGCAATAGA AGCCGGAGTA 
GATATATCGA CATCGGAGAA TGAACAAGTT TGTATGCGTC CCACAAACCG GCTGCCTATA 
ATCGCGATGA CGGCAAATAC TTTAGCAGAG AGTTCAGAAG AATGTTATGC AAATGGTATG 
GACTCGTTTA TTTCGAAACC TGTAACGTTG CAAAAACTGA GAGAGTGTTT GCAACAGTAT 
TTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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HK5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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