Gene Symbol | DME |
Entrez Gene ID | 830335 |
Full Name | HhH-GPD base excision DNA repair family protein |
Synonyms | AT5G04570,AT5G04580,DEMETER,T32M21.160,T32M21_160 |
Gene Type | protein-coding |
Organism | Arabidopsis thaliana(thale cress) |
ORF » Species Summary » Arabidopsis thaliana » DME cDNA ORF clone
Gene Symbol | DME |
Entrez Gene ID | 830335 |
Full Name | HhH-GPD base excision DNA repair family protein |
Synonyms | AT5G04570,AT5G04580,DEMETER,T32M21.160,T32M21_160 |
Gene Type | protein-coding |
Organism | Arabidopsis thaliana(thale cress) |
mRNA | Protein | Name |
---|---|---|
NM_001085058.2 | NP_001078527.1 | HhH-GPD base excision DNA repair family protein |
NM_001342781.1 | NP_001331593.1 | HhH-GPD base excision DNA repair family protein |
NM_120538.2 | NP_196076.2 | HhH-GPD base excision DNA repair family protein |
Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
DME and ROS1 have a significant 5-hydroxymethylcytosine (5hmC) excision activity in vitro, although the study detected no 5hmC in Arabidopsis, suggesting that it is very unlikely for plants to utilize 5hmC as a DNA demethylation intermediate.
Title: Excision of 5-hydroxymethylcytosine by DEMETER family DNA glycosylases.
Histone H1 is involved in DME-mediated DNA methylation and gene regulation at imprinted loci.
Title: Histone H1 affects gene imprinting and DNA methylation in Arabidopsis.
Active DNA demethylation by DEMETER requires SSRP1 function through a distinctly different process from direct DNA methylation control.
Title: HMG domain containing SSRP1 is required for DNA demethylation and genomic imprinting in Arabidopsis.
DME is active and demethylates similar genes and transposons in the genomes of the vegetative and central cells in the male and female gametophytes
Title: Function of the DEMETER DNA glycosylase in the Arabidopsis thaliana male gametophyte.
Analyses indicate that AtLIG1 acts downstream of DME. The removal of methylated cytosine residues by DME involves the creation of DNA single-strand breaks and our results suggest that AtLIG1 repairs these breaks.
Title: DNA LIGASE I exerts a maternal effect on seed development in Arabidopsis thaliana.
The following DME gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DME cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OAb32175 | |
Clone ID Related Accession (Same CDS sequence) | NM_001342781.1 , NM_120538.2 | |
Accession Version | NM_001342781.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-13 | |
Organism | Arabidopsis thaliana(thale cress) | |
Product | HhH-GPD base excision DNA repair family protein | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). |
1 | ATGCAGAGCA TTATGGACTC GTCTGCTGTT AATGCGACGG AAGCTACTGA ACAAAATGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001331593.1 |
CDS | 1069..6258 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001342781.1 |
1 | ATGCAGAGCA TTATGGACTC GTCTGCTGTT AATGCGACGG AAGCTACTGA ACAAAATGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OAb32175 | |
Clone ID Related Accession (Same CDS sequence) | NM_001342781.1 , NM_120538.2 | |
Accession Version | NM_120538.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-13 | |
Organism | Arabidopsis thaliana(thale cress) | |
Product | HhH-GPD base excision DNA repair family protein | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On May 13, 2003 this sequence version replaced NM_120538.1. |
1 | ATGCAGAGCA TTATGGACTC GTCTGCTGTT AATGCGACGG AAGCTACTGA ACAAAATGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_196076.2 |
CDS | 1479..6668 |
Translation |
Target ORF information:
Target ORF information:
|
NM_120538.2 |
1 | ATGCAGAGCA TTATGGACTC GTCTGCTGTT AATGCGACGG AAGCTACTGA ACAAAATGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OAb32176 | |
Clone ID Related Accession (Same CDS sequence) | NM_001085058.2 | |
Accession Version | NM_001085058.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 5964bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-13 | |
Organism | Arabidopsis thaliana(thale cress) | |
Product | HhH-GPD base excision DNA repair family protein | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_001085058.1. |
1 | ATGAATTCGA GGGCTGATCC GGGGGATAGA TATTTTCGAG TTCCTTTGGA GAATCAAACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001078527.1 |
CDS | 290..6253 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001085058.2 |
1 | ATGAATTCGA GGGCTGATCC GGGGGATAGA TATTTTCGAG TTCCTTTGGA GAATCAAACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. |