AT4G27870 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT4G27870
Entrez Gene ID	828900
Full Name	Vacuolar iron transporter (VIT) family protein
Synonyms	T27E11.110,T27E11_110
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001341890.1	NP_001328259.1	Vacuolar iron transporter (VIT) family protein
NM_118925.3	NP_567789.2	Vacuolar iron transporter (VIT) family protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT4G27870 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT4G27870 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16507	NM_118925.3 Latest version!	Arabidopsis thaliana Vacuolar iron transporter (VIT) family protein (AT4G27870), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb39806	NM_001341890.1 Latest version!	Arabidopsis thaliana Vacuolar iron transporter (VIT) family protein (AT4G27870), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT4G27870 ( NM_118925.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567789.2 Arabidopsis thaliana Vacuolar iron transporter (VIT) family protein (AT4G27870), mRNA.

Price & Availability↑

CloneID	OAb16507
Clone ID Related Accession (Same CDS sequence)	NM_118925.3
Accession Version	NM_118925.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Vacuolar iron transporter (VIT) family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118925.2.

ATGGGATCCG CCGCCGAACT CACGGAGCCC ACCACCGGGA CCGAGAAGCT TCACCAGCCA 
ATAGAAGTTG AAGAAGATGA CGAACAAATT GTTGATTTGG AGAGGAAAAC ATTCCGTCAT 
GGAAAGGGAC ACGATACTAG TGTGGACAGT AGTACAATCA CCAACACCAG CTCGTCGTCG 
TCTTCTAGCT TTTCCGGTGA TGGTGGAACG GAAGAGACCC CCGATTTCCA CAGCAACGGC 
GATGGAGAAC ATACCGATTT AGTAAATTTG GAGGTCCCGG AGCTAGAAAC TGAAATCGTA 
TTTCACCAAG AGCATGACGA CGGGAAGAAT TGCATCGTCT TCTTCGACGG AGAACAAGGT 
TCTGAGTCAG CTGGTGCCAT ACCTGATGCT CATGAGGAGT CTCAAACTGC TGACTTGAAT 
GGGGAGCAAA CGCAGTTAGA ACCTGAAAAC GGTTCAACTT CTGAGGATAA CGAAAGATCT 
CGTGAAATTG AAGAAGTACT AGATGGTGAT GTCTCTAAAG ACTTGGATGC TGTTGATCCA 
CTCGCTGGTG AGGTGATAGA GGAGGAGGTG GACTTCGAAG ATGTTGAATA TCATGATGTG 
GAGAATATGA TGGATAAGCA AGAGACACAT GATTTGTACT GTCCAAACTG CGATTCTTGC 
ATCACCAAAA AGGTTATCCT CAAAAGAAGG AAACGGAAGA TCCGTCGTCA TGAGCTGGGA 
GATTCAAAGC GGCCTCATTT GACTGAGCCT CTTTTTCATT CGGAAGACAA TTTGCCATCT 
CTAGATGGCG GTGAAAACTC TGCAAATGAG TCATTTGTCT TCAAGTGCTT GTCATGCTTT 
ACCATATTTA TTCCCAAAGG GGTGAGTTCA AAGCCGATTC CTCCCCGTCA AGGAGTTGAG 
GGGTTAAAGA TTCAGCCAAA CCCTCAAGTG GAAGCTACAG GCGATTCCAA TTGGTTCAGT 
TCAATATTTG GCCTCAACAA AAAGGAATCA GCTATTCAGC AAGGTGGGGC ATCATCTAGT 
GTCTTGGAAG CTAATCCGCC ACCACGTGAG TCCATTGTCC CAGTAGTTAA TCCATCGCGT 
GGTAACCTAA GTCCTATGCG AAAGGATACG ACAGGCTCAG CCGTTGTTCA GCCTGATGCA 
GCCACTTCGA TTCAAGTTGC AAAATCAAAC GATACAAGCG AAATTGTAAA CAATGGAGCT 
ATTGTTGGAG ACGGGCAAAA GTTTCTGGCT CCTATGGTGG AGGAGCAAAC ACAGCAAAAG 
ATTGACAATG ATGATTCTAG TACAGCAGAC GGGAATCACA CGTCAGACAA AGGACGTCTC 
TCCCCAATTC AACCATCTCA TGGCATGAGT ATACTAAACA CAGTGACTAA CGGACCCGAC 
GGATTGAAAG TAGAAACTAC AATTCATGAA GAAGGTGCCC CTCTTCTGTT TGAAGGAAAA 
GACACTCCTG ACACAAGCAC GGCAGATTTT GGTCTGACCA AAGTAACAGG TGTGATGGAC 
ACAGGTGACA GAGGAGTTAT TACCGGTCCA GCAAATCCTG AAATTGATAT TTCAGCAGGG 
AATTTGCTGG AAGAAGGGTC TCTAAGGGAA CCGTTAATGA GACGAGTTGT TGTTCAAGGA 
CGTAAACTGG AAATCTTGAA AAGCATTGTC TACGGAGGTC TACTAGAAGC CATCACAAGC 
CTTGGTGTTA TTTCATCTGC CGCTGGTTCT GGTGCTTCAA TGTTGAATAT TCTGGTCTTG 
GGGCTTGCAA ACTTGCTTGG CGGGCTTATT CTTATTATTC ATAATCTCCA AGAACTTAGA 
GAGGAGGAAC CCATAAGAAC AACAACAGAA GATAACCAGA CTAACGGTAG AGAAGAAGAA 
GAAGGTCGGT ATAAGCGACT CCTTGGACGA AGAGAAAATT TCACACTTCA CGCAACAGTC 
GCAATCTTAT CCTTCATAAT CACCGGAATA CTCCCTCCTG TAGTCTACTA TTTCTCATTC 
AGTGAAAAAC ACAACAAAGA CTACAAAGTT GCTTCAGTTT TCGGGGCATC TCTATTCTGC 
ATCGTCTTGC TCGCCATAGC CAAAGCTCAT GTGAGGTACC CGAGAGGCAG CTACCTGAAG 
AGCATTCTGT ACTACGGGTC AATTGCTGTA TCGGTGTCGG GGATTTCTTA TGTGGTTGGT 
AACTTTCTGG AACAGTTGCT TGAGAAGCAT GGATGGTCTG ATGGATCGGA AACACCTGTG 
GGACAGATGA TGCTTTCATC ACTTATGGGA AGAAAAGCCG GCTTTGGATA CTCATCATCG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567789.2
CDS	283..2568
Translation	MGSAAELTEPTTGTEKLHQPIEVEEDDEQIVDLERKTFRHGKGHDTSVDSSTITNTSSSSSSSFSGDGGTEETPDFHSNGDGEHTDLVNLEVPELETEIVFHQEHDDGKNCIVFFDGEQGSESAGAIPDAHEESQTADLNGEQTQLEPENGSTSEDNERSREIEEVLDGDVSKDLDAVDPLAGEVIEEEVDFEDVEYHDVENMMDKQETHDLYCPNCDSCITKKVILKRRKRKIRRHELGDSKRPHLTEPLFHSEDNLPSLDGGENSANESFVFKCLSCFTIFIPKGVSSKPIPPRQGVEGLKIQPNPQVEATGDSNWFSSIFGLNKKESAIQQGGASSSVLEANPPPRESIVPVVNPSRGNLSPMRKDTTGSAVVQPDAATSIQVAKSNDTSEIVNNGAIVGDGQKFLAPMVEEQTQQKIDNDDSSTADGNHTSDKGRLSPIQPSHGMSILNTVTNGPDGLKVETTIHEEGAPLLFEGKDTPDTSTADFGLTKVTGVMDTGDRGVITGPANPEIDISAGNLLEEGSLREPLMRRVVVQGRKLEILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGGLILIIHNLQELREEEPIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIITGILPPVVYYFSFSEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYPRGSYLKSILYYGSIAVSVSGISYVVGNFLEQLLEKHGWSDGSETPVGQMMLSSLMGRKAGFGYSSSY

Target ORF information:

RefSeq Version	NM_118925.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Vacuolar iron transporter (VIT) family protein (AT4G27870), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118925.3

ORF Insert Sequence:

ATGGGATCCG CCGCCGAACT CACGGAGCCC ACCACCGGGA CCGAGAAGCT TCACCAGCCA 
ATAGAAGTTG AAGAAGATGA CGAACAAATT GTTGATTTGG AGAGGAAAAC ATTCCGTCAT 
GGAAAGGGAC ACGATACTAG TGTGGACAGT AGTACAATCA CCAACACCAG CTCGTCGTCG 
TCTTCTAGCT TTTCCGGTGA TGGTGGAACG GAAGAGACCC CCGATTTCCA CAGCAACGGC 
GATGGAGAAC ATACCGATTT AGTAAATTTG GAGGTCCCGG AGCTAGAAAC TGAAATCGTA 
TTTCACCAAG AGCATGACGA CGGGAAGAAT TGCATCGTCT TCTTCGACGG AGAACAAGGT 
TCTGAGTCAG CTGGTGCCAT ACCTGATGCT CATGAGGAGT CTCAAACTGC TGACTTGAAT 
GGGGAGCAAA CGCAGTTAGA ACCTGAAAAC GGTTCAACTT CTGAGGATAA CGAAAGATCT 
CGTGAAATTG AAGAAGTACT AGATGGTGAT GTCTCTAAAG ACTTGGATGC TGTTGATCCA 
CTCGCTGGTG AGGTGATAGA GGAGGAGGTG GACTTCGAAG ATGTTGAATA TCATGATGTG 
GAGAATATGA TGGATAAGCA AGAGACACAT GATTTGTACT GTCCAAACTG CGATTCTTGC 
ATCACCAAAA AGGTTATCCT CAAAAGAAGG AAACGGAAGA TCCGTCGTCA TGAGCTGGGA 
GATTCAAAGC GGCCTCATTT GACTGAGCCT CTTTTTCATT CGGAAGACAA TTTGCCATCT 
CTAGATGGCG GTGAAAACTC TGCAAATGAG TCATTTGTCT TCAAGTGCTT GTCATGCTTT 
ACCATATTTA TTCCCAAAGG GGTGAGTTCA AAGCCGATTC CTCCCCGTCA AGGAGTTGAG 
GGGTTAAAGA TTCAGCCAAA CCCTCAAGTG GAAGCTACAG GCGATTCCAA TTGGTTCAGT 
TCAATATTTG GCCTCAACAA AAAGGAATCA GCTATTCAGC AAGGTGGGGC ATCATCTAGT 
GTCTTGGAAG CTAATCCGCC ACCACGTGAG TCCATTGTCC CAGTAGTTAA TCCATCGCGT 
GGTAACCTAA GTCCTATGCG AAAGGATACG ACAGGCTCAG CCGTTGTTCA GCCTGATGCA 
GCCACTTCGA TTCAAGTTGC AAAATCAAAC GATACAAGCG AAATTGTAAA CAATGGAGCT 
ATTGTTGGAG ACGGGCAAAA GTTTCTGGCT CCTATGGTGG AGGAGCAAAC ACAGCAAAAG 
ATTGACAATG ATGATTCTAG TACAGCAGAC GGGAATCACA CGTCAGACAA AGGACGTCTC 
TCCCCAATTC AACCATCTCA TGGCATGAGT ATACTAAACA CAGTGACTAA CGGACCCGAC 
GGATTGAAAG TAGAAACTAC AATTCATGAA GAAGGTGCCC CTCTTCTGTT TGAAGGAAAA 
GACACTCCTG ACACAAGCAC GGCAGATTTT GGTCTGACCA AAGTAACAGG TGTGATGGAC 
ACAGGTGACA GAGGAGTTAT TACCGGTCCA GCAAATCCTG AAATTGATAT TTCAGCAGGG 
AATTTGCTGG AAGAAGGGTC TCTAAGGGAA CCGTTAATGA GACGAGTTGT TGTTCAAGGA 
CGTAAACTGG AAATCTTGAA AAGCATTGTC TACGGAGGTC TACTAGAAGC CATCACAAGC 
CTTGGTGTTA TTTCATCTGC CGCTGGTTCT GGTGCTTCAA TGTTGAATAT TCTGGTCTTG 
GGGCTTGCAA ACTTGCTTGG CGGGCTTATT CTTATTATTC ATAATCTCCA AGAACTTAGA 
GAGGAGGAAC CCATAAGAAC AACAACAGAA GATAACCAGA CTAACGGTAG AGAAGAAGAA 
GAAGGTCGGT ATAAGCGACT CCTTGGACGA AGAGAAAATT TCACACTTCA CGCAACAGTC 
GCAATCTTAT CCTTCATAAT CACCGGAATA CTCCCTCCTG TAGTCTACTA TTTCTCATTC 
AGTGAAAAAC ACAACAAAGA CTACAAAGTT GCTTCAGTTT TCGGGGCATC TCTATTCTGC 
ATCGTCTTGC TCGCCATAGC CAAAGCTCAT GTGAGGTACC CGAGAGGCAG CTACCTGAAG 
AGCATTCTGT ACTACGGGTC AATTGCTGTA TCGGTGTCGG GGATTTCTTA TGTGGTTGGT 
AACTTTCTGG AACAGTTGCT TGAGAAGCAT GGATGGTCTG ATGGATCGGA AACACCTGTG 
GGACAGATGA TGCTTTCATC ACTTATGGGA AGAAAAGCCG GCTTTGGATA CTCATCATCG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT4G27870 ( NM_001341890.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001328259.1 Arabidopsis thaliana Vacuolar iron transporter (VIT) family protein (AT4G27870), mRNA.

Price & Availability↑

CloneID	OAb39806
Clone ID Related Accession (Same CDS sequence)	NM_001341890.1
Accession Version	NM_001341890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Vacuolar iron transporter (VIT) family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGGATCCG CCGCCGAACT CACGGAGCCC ACCACCGGGA CCGAGAAGCT TCACCAGCCA 
ATAGAAGTTG AAGAAGATGA CGAACAAATT GTTGATTTGG AGAGGAAAAC ATTCCGTCAT 
GGAAAGGGAC ACGATACTAG TGTGGACAGT AGTACAATCA CCAACACCAG CTCGTCGTCG 
TCTTCTAGCT TTTCCGGTGA TGGTGGAACG GAAGAGACCC CCGATTTCCA CAGCAACGGC 
GATGGAGAAC ATACCGATTT AGTAAATTTG GAGGTCCCGG AGCTAGAAAC TGAAATCGTA 
TTTCACCAAG AGCATGACGA CGGGAAGAAT TGCATCGTCT TCTTCGACGG AGAACAAGGA 
ATTTGGAAAT GTCGCCACTG CGATTGGACC TACAAAGAGG GATCTTTGTT ATGCAAAGGT 
TCTGAGTCAG CTGGTGCCAT ACCTGATGCT CATGAGGAGT CTCAAACTGC TGACTTGAAT 
GGGGAGCAAA CGCAGTTAGA ACCTGAAAAC GGTTCAACTT CTGAGGATAA CGAAAGATCT 
CGTGAAATTG AAGAAGTACT AGATGGTGAT GTCTCTAAAG ACTTGGATGC TGTTGATCCA 
CTCGCTGGTG AGGTGATAGA GGAGGAGGTG GACTTCGAAG ATGTTGAATA TCATGATGTG 
GAGAATATGA TGGATAAGCA AGAGACACAT GATTTGTACT GTCCAAACTG CGATTCTTGC 
ATCACCAAAA AGGTTATCCT CAAAAGAAGG AAACGGAAGA TCCGTCGTCA TGAGCTGGGA 
GATTCAAAGC GGCCTCATTT GACTGAGCCT CTTTTTCATT CGGAAGACAA TTTGCCATCT 
CTAGATGGCG GTGAAAACTC TGCAAATGAG TCATTTGTCT TCAAGTGCTT GTCATGCTTT 
ACCATATTTA TTCCCAAAGG GGTGAGTTCA AAGCCGATTC CTCCCCGTCA AGGAGTTGAG 
GGGTTAAAGA TTCAGCCAAA CCCTCAAGTG GAAGCTACAG GCGATTCCAA TTGGTTCAGT 
TCAATATTTG GCCTCAACAA AAAGGAATCA GCTATTCAGC AAGGTGGGGC ATCATCTAGT 
GTCTTGGAAG CTAATCCGCC ACCACGTGAG TCCATTGTCC CAGTAGTTAA TCCATCGCGT 
GGTAACCTAA GTCCTATGCG AAAGGATACG ACAGGCTCAG CCGTTGTTCA GCCTGATGCA 
GCCACTTCGA TTCAAGTTGC AAAATCAAAC GATACAAGCG AAATTGTAAA CAATGGAGCT 
ATTGTTGGAG ACGGGCAAAA GTTTCTGGCT CCTATGGTGG AGGAGCAAAC ACAGCAAAAG 
ATTGACAATG ATGATTCTAG TACAGCAGAC GGGAATCACA CGTCAGACAA AGGACGTCTC 
TCCCCAATTC AACCATCTCA TGGCATGAGT ATACTAAACA CAGTGACTAA CGGACCCGAC 
GGATTGAAAG TAGAAACTAC AATTCATGAA GAAGGTGCCC CTCTTCTGTT TGAAGGAAAA 
GACACTCCTG ACACAAGCAC GGCAGATTTT GGTCTGACCA AAGTAACAGG TGTGATGGAC 
ACAGGTGACA GAGGAGTTAT TACCGGTCCA GCAAATCCTG AAATTGATAT TTCAGCAGGG 
AATTTGCTGG AAGAAGGGTC TCTAAGGGAA CCGTTAATGA GACGAGTTGT TGTTCAAGGA 
CGTAAACTGG AAATCTTGAA AAGCATTGTC TACGGAGGTC TACTAGAAGC CATCACAAGC 
CTTGGTGTTA TTTCATCTGC CGCTGGTTCT GGTGCTTCAA TGTTGAATAT TCTGGTCTTG 
GGGCTTGCAA ACTTGCTTGG CGGGCTTATT CTTATTATTC ATAATCTCCA AGAACTTAGA 
GAGGAGGAAC CCATAAGAAC AACAACAGAA GATAACCAGA CTAACGGTAG AGAAGAAGAA 
GAAGGTCGGT ATAAGCGACT CCTTGGACGA AGAGAAAATT TCACACTTCA CGCAACAGTC 
GCAATCTTAT CCTTCATAAT CACCGGAATA CTCCCTCCTG TAGTCTACTA TTTCTCATTC 
AGTGAAAAAC ACAACAAAGA CTACAAAGTT GCTTCAGTTT TCGGGGCATC TCTATTCTGC 
ATCGTCTTGC TCGCCATAGC CAAAGCTCAT GTGAGGTACC CGAGAGGCAG CTACCTGAAG 
AGCATTCTGT ACTACGGGTC AATTGCTGTA TCGGTGTCGG GGATTTCTTA TGTGGTTGGT 
AACTTTCTGG AACAGTTGCT TGAGAAGCAT GGATGGTCTG ATGGATCGGA AACACCTGTG 
GGACAGATGA TGCTTTCATC ACTTATGGGA AGAAAAGCCG GCTTTGGATA CTCATCATCG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001328259.1
CDS	229..2574
Translation	MGSAAELTEPTTGTEKLHQPIEVEEDDEQIVDLERKTFRHGKGHDTSVDSSTITNTSSSSSSSFSGDGGTEETPDFHSNGDGEHTDLVNLEVPELETEIVFHQEHDDGKNCIVFFDGEQGIWKCRHCDWTYKEGSLLCKGSESAGAIPDAHEESQTADLNGEQTQLEPENGSTSEDNERSREIEEVLDGDVSKDLDAVDPLAGEVIEEEVDFEDVEYHDVENMMDKQETHDLYCPNCDSCITKKVILKRRKRKIRRHELGDSKRPHLTEPLFHSEDNLPSLDGGENSANESFVFKCLSCFTIFIPKGVSSKPIPPRQGVEGLKIQPNPQVEATGDSNWFSSIFGLNKKESAIQQGGASSSVLEANPPPRESIVPVVNPSRGNLSPMRKDTTGSAVVQPDAATSIQVAKSNDTSEIVNNGAIVGDGQKFLAPMVEEQTQQKIDNDDSSTADGNHTSDKGRLSPIQPSHGMSILNTVTNGPDGLKVETTIHEEGAPLLFEGKDTPDTSTADFGLTKVTGVMDTGDRGVITGPANPEIDISAGNLLEEGSLREPLMRRVVVQGRKLEILKSIVYGGLLEAITSLGVISSAAGSGASMLNILVLGLANLLGGLILIIHNLQELREEEPIRTTTEDNQTNGREEEEGRYKRLLGRRENFTLHATVAILSFIITGILPPVVYYFSFSEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYPRGSYLKSILYYGSIAVSVSGISYVVGNFLEQLLEKHGWSDGSETPVGQMMLSSLMGRKAGFGYSSSY

Target ORF information:

RefSeq Version	NM_001341890.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Vacuolar iron transporter (VIT) family protein (AT4G27870), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341890.1

ORF Insert Sequence:

ATGGGATCCG CCGCCGAACT CACGGAGCCC ACCACCGGGA CCGAGAAGCT TCACCAGCCA 
ATAGAAGTTG AAGAAGATGA CGAACAAATT GTTGATTTGG AGAGGAAAAC ATTCCGTCAT 
GGAAAGGGAC ACGATACTAG TGTGGACAGT AGTACAATCA CCAACACCAG CTCGTCGTCG 
TCTTCTAGCT TTTCCGGTGA TGGTGGAACG GAAGAGACCC CCGATTTCCA CAGCAACGGC 
GATGGAGAAC ATACCGATTT AGTAAATTTG GAGGTCCCGG AGCTAGAAAC TGAAATCGTA 
TTTCACCAAG AGCATGACGA CGGGAAGAAT TGCATCGTCT TCTTCGACGG AGAACAAGGA 
ATTTGGAAAT GTCGCCACTG CGATTGGACC TACAAAGAGG GATCTTTGTT ATGCAAAGGT 
TCTGAGTCAG CTGGTGCCAT ACCTGATGCT CATGAGGAGT CTCAAACTGC TGACTTGAAT 
GGGGAGCAAA CGCAGTTAGA ACCTGAAAAC GGTTCAACTT CTGAGGATAA CGAAAGATCT 
CGTGAAATTG AAGAAGTACT AGATGGTGAT GTCTCTAAAG ACTTGGATGC TGTTGATCCA 
CTCGCTGGTG AGGTGATAGA GGAGGAGGTG GACTTCGAAG ATGTTGAATA TCATGATGTG 
GAGAATATGA TGGATAAGCA AGAGACACAT GATTTGTACT GTCCAAACTG CGATTCTTGC 
ATCACCAAAA AGGTTATCCT CAAAAGAAGG AAACGGAAGA TCCGTCGTCA TGAGCTGGGA 
GATTCAAAGC GGCCTCATTT GACTGAGCCT CTTTTTCATT CGGAAGACAA TTTGCCATCT 
CTAGATGGCG GTGAAAACTC TGCAAATGAG TCATTTGTCT TCAAGTGCTT GTCATGCTTT 
ACCATATTTA TTCCCAAAGG GGTGAGTTCA AAGCCGATTC CTCCCCGTCA AGGAGTTGAG 
GGGTTAAAGA TTCAGCCAAA CCCTCAAGTG GAAGCTACAG GCGATTCCAA TTGGTTCAGT 
TCAATATTTG GCCTCAACAA AAAGGAATCA GCTATTCAGC AAGGTGGGGC ATCATCTAGT 
GTCTTGGAAG CTAATCCGCC ACCACGTGAG TCCATTGTCC CAGTAGTTAA TCCATCGCGT 
GGTAACCTAA GTCCTATGCG AAAGGATACG ACAGGCTCAG CCGTTGTTCA GCCTGATGCA 
GCCACTTCGA TTCAAGTTGC AAAATCAAAC GATACAAGCG AAATTGTAAA CAATGGAGCT 
ATTGTTGGAG ACGGGCAAAA GTTTCTGGCT CCTATGGTGG AGGAGCAAAC ACAGCAAAAG 
ATTGACAATG ATGATTCTAG TACAGCAGAC GGGAATCACA CGTCAGACAA AGGACGTCTC 
TCCCCAATTC AACCATCTCA TGGCATGAGT ATACTAAACA CAGTGACTAA CGGACCCGAC 
GGATTGAAAG TAGAAACTAC AATTCATGAA GAAGGTGCCC CTCTTCTGTT TGAAGGAAAA 
GACACTCCTG ACACAAGCAC GGCAGATTTT GGTCTGACCA AAGTAACAGG TGTGATGGAC 
ACAGGTGACA GAGGAGTTAT TACCGGTCCA GCAAATCCTG AAATTGATAT TTCAGCAGGG 
AATTTGCTGG AAGAAGGGTC TCTAAGGGAA CCGTTAATGA GACGAGTTGT TGTTCAAGGA 
CGTAAACTGG AAATCTTGAA AAGCATTGTC TACGGAGGTC TACTAGAAGC CATCACAAGC 
CTTGGTGTTA TTTCATCTGC CGCTGGTTCT GGTGCTTCAA TGTTGAATAT TCTGGTCTTG 
GGGCTTGCAA ACTTGCTTGG CGGGCTTATT CTTATTATTC ATAATCTCCA AGAACTTAGA 
GAGGAGGAAC CCATAAGAAC AACAACAGAA GATAACCAGA CTAACGGTAG AGAAGAAGAA 
GAAGGTCGGT ATAAGCGACT CCTTGGACGA AGAGAAAATT TCACACTTCA CGCAACAGTC 
GCAATCTTAT CCTTCATAAT CACCGGAATA CTCCCTCCTG TAGTCTACTA TTTCTCATTC 
AGTGAAAAAC ACAACAAAGA CTACAAAGTT GCTTCAGTTT TCGGGGCATC TCTATTCTGC 
ATCGTCTTGC TCGCCATAGC CAAAGCTCAT GTGAGGTACC CGAGAGGCAG CTACCTGAAG 
AGCATTCTGT ACTACGGGTC AATTGCTGTA TCGGTGTCGG GGATTTCTTA TGTGGTTGGT 
AACTTTCTGG AACAGTTGCT TGAGAAGCAT GGATGGTCTG ATGGATCGGA AACACCTGTG 
GGACAGATGA TGCTTTCATC ACTTATGGGA AGAAAAGCCG GCTTTGGATA CTCATCATCG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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AT4G27870 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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