PPI1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPI1
Entrez Gene ID	828859
Full Name	proton pump interactor 1
Synonyms	F27G19.100,F27G19_100,proton pump interactor 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_118885.4	NP_194480.2	proton pump interactor 1
NM_001341859.1	NP_001328990.1	proton pump interactor 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os09g0346400	NP_001062938.1
Arabidopsis thaliana (thale cress)	PPI1	NP_194480.2
Oryza sativa (rice)	Os08g0390000	NP_001061721.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PPI1 is localised at the endoplasmic reticulum, from which it is recruited to the plasma membrane for interaction with H(+)-ATPase.
Title: Intracellular localisation of PPI1 (proton pump interactor, isoform 1), a regulatory protein of the plasma membrane H(+)-ATPase of Arabidopsis thaliana.

Data show that Ppi1 expression is strong in root and shoot vascular systems, particularly in meristematic and sink tissues, as well as in pollen, stigmas and siliques, but not in developing embryos.
Title: The proton pump interactor (Ppi) gene family of Arabidopsis thaliana: expression pattern of Ppi1 and characterisation of knockout mutants for Ppi1 and 2.

PPI1 can bind the H(+)-ATPase independently of the C-terminus conformation, but is not able to suppress the C-terminus auto-inhibitory action.
Title: Characterization of the interaction between the plasma membrane H-ATPase of Arabidopsis thaliana and a novel interactor (PPI1).

The following PPI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16473	NM_118885.4 Latest version!	Arabidopsis thaliana proton pump interactor 1 (PPI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb39789	NM_001341859.1 Latest version!	Arabidopsis thaliana proton pump interactor 1 (PPI1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPI1 ( NM_118885.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194480.2 Arabidopsis thaliana proton pump interactor 1 (PPI1), mRNA.

Price & Availability↑

CloneID	OAb16473
Clone ID Related Accession (Same CDS sequence)	NM_118885.4
Accession Version	NM_118885.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	proton pump interactor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118885.3.

ATGGGTGTTG AAGTTGTAAA CTCTGGTGGA TTTGAGGTTG CTCCGGCTCC TTTCGAGGGG 
AAACCTGAGA AGAACGGGAA GCTGGACCAA GGAAAAGGAG ATGATGCCCC CATCAATTTT 
GGTTCAGTCG GTGAGCTACC AAAGAATGCC GAAGAGAATA ATAACAAAGT GGTCAACTCT 
GATGCACCCA AAAATGCAGC AGAAGAGTGG CCTGTGGCTA AGCAGATCCA CTCTTTCTAC 
CTTGTTAAAT ACCGTTCTTA CGCTGACCCC AAAATTAAAG CTAAGCTTGA TCTAGCTGAT 
AAAGAGCTTG AAAAGTTGAA CAAAGCTCGG ACTGGAGTTT TGGACAAGTT GAGAGCTAAG 
AGGGCCGAGA GATCAGAGTT ATTTGATCTG CTGGACCCGT TGAAGTCAGA GCGTAAGGGA 
TTCAACACAA TGTTTGATGA GAAAAGAAAG GAAATGGAAC CATTGCAGCA AGCACTGGGA 
AAGTTAAGGA GCAATGATGG TGGGAGTGCT CGTGGCCCTG CTATCTGTTC CTCCGAGGAA 
GAGCTGAATA GTATGATATA CAGCTATCAG TATCGGATTC AACATGAAAG CATTCCGTTA 
ACCGAGGAGA AGCAGATTCT CAAAGAGATC AGGCTGCTTG AAGGGACAAG AGATAAGGTC 
ATTGCTAATG CCGCTATGAG AGCCAAAATC AAAGAATCTA TGGGTCAGAA GGATGATATC 
CAAGGTCAAG TTAAGTTAAT GGGTGCTGGT TTGGATGGAG TGAAAAAGGA GAGGCAAGCA 
ATTTCAGCAA GGATTAATGA GCTGAGTGAG AAGTTGAAAG CTACCAAAGA TGAGATTACG 
GTACTGGAAA ATGAGCTCAA GACTGTGAGC GAAAAGAGAG ACAAAGCCTA TTCAAACATT 
CATGACCTCA GGAGGCAACG CGATGAGACG AATTCCGAGT ATTACCAAAA CCGTACTGTG 
TTGAACAAAG CTAGAGACTT GGCTGCACAA AAGAACATAA GCGAGCTTGA GGCGCTAGCC 
AATGCTGAGG TTGAGAAATT TATATCCCTT TGGTGCAGTA AGAAGAATTT CAGAGAAGAT 
TATGAGAAGA GAATCTTGCA ATCACTTGAT TCTAGGCAGC TGAGCCGAGA TGGACGGATG 
AGGAACCCCG ATGAGAAGCC TCTGATTGCT CCAGAGGCAG CACCATCAAA GGCAACGCCT 
TCTGAAACCG AGGTTGTCCC TAAAGCTAAG GCAAAGCCGC AGCCAAAGGA GGAACCAGTT 
TCTGCACCTA AACCAGATGC TACTGTTGCT CAGAATACTG AGAAAGCTAA AGATGCAGTA 
AAAGTAAAGA ATGTTGCTGA TGATGATGAT GATGAAGTAT ATGGTCTTGG AAAGCCGCAG 
AAGGAAGAAA AGCCGGTTGA TGCAGCTACG GCGAAGGAAA TGAGAAAGCA AGAGGAGATT 
GCTAAAGCCA AGCAAGCCAT GGAAAGGAAG AAGAAGCTGG CAGAGAAAGC TGCTGCTAAA 
GCTGCTATAA GAGCTCAAAA GGAAGCTGAG AAGAAAGAAA AGAAGGAGCA AGAGAAGAAG 
GCCAAGAAGA AAACCGGAGG CAACACAGAA ACCGAGACTG AGGAAGTTCC AGAAGCTTCT 
GAGGAGGAGA TTGAAGCTCC GGTGCAGGAG GAGAAACCGC AGAAGGAAAA AGTCTTCAAG 
GAGAAACCAA TAAGGAACAG GACCCGCGGT AGGGGACCAG AAACGATCCC AAGAGCGATC 
CTTAAGCGTA AGAAGTCGAC TAATTACTGG GTTTATGCTG CTCCAGCGGC TCTGGTGGTA 
CTGTTGCTTC TTGTCTTGGG TTACTACTAC GTTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194480.2
CDS	287..2125
Translation	MGVEVVNSGGFEVAPAPFEGKPEKNGKLDQGKGDDAPINFGSVGELPKNAEENNNKVVNSDAPKNAAEEWPVAKQIHSFYLVKYRSYADPKIKAKLDLADKELEKLNKARTGVLDKLRAKRAERSELFDLLDPLKSERKGFNTMFDEKRKEMEPLQQALGKLRSNDGGSARGPAICSSEEELNSMIYSYQYRIQHESIPLTEEKQILKEIRLLEGTRDKVIANAAMRAKIKESMGQKDDIQGQVKLMGAGLDGVKKERQAISARINELSEKLKATKDEITVLENELKTVSEKRDKAYSNIHDLRRQRDETNSEYYQNRTVLNKARDLAAQKNISELEALANAEVEKFISLWCSKKNFREDYEKRILQSLDSRQLSRDGRMRNPDEKPLIAPEAAPSKATPSETEVVPKAKAKPQPKEEPVSAPKPDATVAQNTEKAKDAVKVKNVADDDDDEVYGLGKPQKEEKPVDAATAKEMRKQEEIAKAKQAMERKKKLAEKAAAKAAIRAQKEAEKKEKKEQEKKAKKKTGGNTETETEEVPEASEEEIEAPVQEEKPQKEKVFKEKPIRNRTRGRGPETIPRAILKRKKSTNYWVYAAPAALVVLLLLVLGYYYVL

Target ORF information:

RefSeq Version	NM_118885.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana proton pump interactor 1 (PPI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118885.4

ORF Insert Sequence:

ATGGGTGTTG AAGTTGTAAA CTCTGGTGGA TTTGAGGTTG CTCCGGCTCC TTTCGAGGGG 
AAACCTGAGA AGAACGGGAA GCTGGACCAA GGAAAAGGAG ATGATGCCCC CATCAATTTT 
GGTTCAGTCG GTGAGCTACC AAAGAATGCC GAAGAGAATA ATAACAAAGT GGTCAACTCT 
GATGCACCCA AAAATGCAGC AGAAGAGTGG CCTGTGGCTA AGCAGATCCA CTCTTTCTAC 
CTTGTTAAAT ACCGTTCTTA CGCTGACCCC AAAATTAAAG CTAAGCTTGA TCTAGCTGAT 
AAAGAGCTTG AAAAGTTGAA CAAAGCTCGG ACTGGAGTTT TGGACAAGTT GAGAGCTAAG 
AGGGCCGAGA GATCAGAGTT ATTTGATCTG CTGGACCCGT TGAAGTCAGA GCGTAAGGGA 
TTCAACACAA TGTTTGATGA GAAAAGAAAG GAAATGGAAC CATTGCAGCA AGCACTGGGA 
AAGTTAAGGA GCAATGATGG TGGGAGTGCT CGTGGCCCTG CTATCTGTTC CTCCGAGGAA 
GAGCTGAATA GTATGATATA CAGCTATCAG TATCGGATTC AACATGAAAG CATTCCGTTA 
ACCGAGGAGA AGCAGATTCT CAAAGAGATC AGGCTGCTTG AAGGGACAAG AGATAAGGTC 
ATTGCTAATG CCGCTATGAG AGCCAAAATC AAAGAATCTA TGGGTCAGAA GGATGATATC 
CAAGGTCAAG TTAAGTTAAT GGGTGCTGGT TTGGATGGAG TGAAAAAGGA GAGGCAAGCA 
ATTTCAGCAA GGATTAATGA GCTGAGTGAG AAGTTGAAAG CTACCAAAGA TGAGATTACG 
GTACTGGAAA ATGAGCTCAA GACTGTGAGC GAAAAGAGAG ACAAAGCCTA TTCAAACATT 
CATGACCTCA GGAGGCAACG CGATGAGACG AATTCCGAGT ATTACCAAAA CCGTACTGTG 
TTGAACAAAG CTAGAGACTT GGCTGCACAA AAGAACATAA GCGAGCTTGA GGCGCTAGCC 
AATGCTGAGG TTGAGAAATT TATATCCCTT TGGTGCAGTA AGAAGAATTT CAGAGAAGAT 
TATGAGAAGA GAATCTTGCA ATCACTTGAT TCTAGGCAGC TGAGCCGAGA TGGACGGATG 
AGGAACCCCG ATGAGAAGCC TCTGATTGCT CCAGAGGCAG CACCATCAAA GGCAACGCCT 
TCTGAAACCG AGGTTGTCCC TAAAGCTAAG GCAAAGCCGC AGCCAAAGGA GGAACCAGTT 
TCTGCACCTA AACCAGATGC TACTGTTGCT CAGAATACTG AGAAAGCTAA AGATGCAGTA 
AAAGTAAAGA ATGTTGCTGA TGATGATGAT GATGAAGTAT ATGGTCTTGG AAAGCCGCAG 
AAGGAAGAAA AGCCGGTTGA TGCAGCTACG GCGAAGGAAA TGAGAAAGCA AGAGGAGATT 
GCTAAAGCCA AGCAAGCCAT GGAAAGGAAG AAGAAGCTGG CAGAGAAAGC TGCTGCTAAA 
GCTGCTATAA GAGCTCAAAA GGAAGCTGAG AAGAAAGAAA AGAAGGAGCA AGAGAAGAAG 
GCCAAGAAGA AAACCGGAGG CAACACAGAA ACCGAGACTG AGGAAGTTCC AGAAGCTTCT 
GAGGAGGAGA TTGAAGCTCC GGTGCAGGAG GAGAAACCGC AGAAGGAAAA AGTCTTCAAG 
GAGAAACCAA TAAGGAACAG GACCCGCGGT AGGGGACCAG AAACGATCCC AAGAGCGATC 
CTTAAGCGTA AGAAGTCGAC TAATTACTGG GTTTATGCTG CTCCAGCGGC TCTGGTGGTA 
CTGTTGCTTC TTGTCTTGGG TTACTACTAC GTTCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPI1 ( NM_001341859.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001328990.1 Arabidopsis thaliana proton pump interactor 1 (PPI1), partial mRNA.

Price & Availability↑

CloneID	OAb39789
Clone ID Related Accession (Same CDS sequence)	NM_001341859.1
Accession Version	NM_001341859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	proton pump interactor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). COMPLETENESS: incomplete on the 3' end.

ATGGGTGTTG AAGTTGTAAA CTCTGGTGGA TTTGAGGTTG CTCCGGCTCC TTTCGAGGGG 
AAACCTGAGA AGAACGGGAA GCTGGACCAA GGAAAAGGAG ATGATGCCCC CATCAATTTT 
GGTTCAGTCG GTGAGCTACC AAAGAATGCC GAAGAGAATA ATAACAAAGT GGTCAACTCT 
GATGCACCCA AAAATGCAGC AGAAGAGTGG CCTGTGGCTA AGCAGATCCA CTCTTTCTAC 
CTTGTTAAAT ACCGTTCTTA CGCTGACCCC AAAATTAAAG CTAAGCTTGA TCTAGCTGAT 
AAAGAGCTTG AAAAGTTGAA CAAAGCTCGG ACTGGAGTTT TGGACAAGTT GAGAGCTAAG 
AGGGCCGAGA GATCAGAGTT ATTTGATCTG CTGGACCCGT TGAAGTCAGA GCGTAAGGGA 
TTCAACACAA TGTTTGATGA GAAAAGAAAG GAAATGGAAC CATTGCAGCA AGCACTGGGA 
AAGTTAAGGA GCAATGATGG TGGGAGTGCT CGTGGCCCTG CTATCTGTTC CTCCGAGGAA 
GAGCTGAATA GTATGATATA CAGCTATCAG TATCGGATTC AACATGAAAG CATTCCGTTA 
ACCGAGGAGA AGCAGATTCT CAAAGAGATC AGGCTGCTTG AAGGGACAAG AGATAAGGTC 
ATTGCTAATG CCGCTATGAG AGCCAAAATC AAAGAATCTA TGGGTCAGAA GGATGATATC 
CAAGGTCAAG TTAAGTTAAT GGGTGCTGGT TTGGATGGAG TGAAAAAGGA GAGGCAAGCA 
ATTTCAGCAA GGATTAATGA GCTGAGTGAG AAGTTGAAAG CTACCAAAGA TGAGATTACG 
GTACTGGAAA ATGAGCTCAA GACTGTGAGC GAAAAGAGAG ACAAAGCCTA TTCAAACATT 
CATGACCTCA GGAGGCAACG CGATGAGACG AATTCCGAGT ATTACCAAAA CCGTACTGTG 
TTGAACAAAG CTAGAGACTT GGCTGCACAA AAGAACATAA GCGAGCTTGA GGCGCTAGCC 
AATGCTGAGG TTGAGAAATT TATATCCCTT TGGTGCAGTA AGAAGAATTT CAGAGAAGAT 
TATGAGAAGA GAATCTTGCA ATCACTTGAT TCTAGGCAGC TGAGCCGAGA TGGACGGATG 
AGGAACCCCG ATGAGAAGCC TCTGATTGCT CCAGAGGCAG CACCATCAAA GGCAACGCCT 
TCTGAAACCG AGGTTGTCCC TAAAGCTAAG GCAAAGCCGC AGCCAAAGGA GGAACCAGTT 
TCTGCACCTA AACCAGATGC TACTGTTGCT CAGAATACTG AGAAAGCTAA AGATGCAGTA 
AAAGTAAAGA ATGTTGCTGA TGATGATGAT GATGAAGTAT ATGGTCTTGG AAAGCCGCAG 
AAGGAAGAAA AGCCGGTTGA TGCAGCTACG GCGAAGGAAA TGAGAAAGCA AGAGGAGATT 
GCTAAAGCCA AGCAAGCCAT GGAAAGGAAG AAGAAGCTGG CAGAGAAAGC TGCTGCTAAA 
GCTGCTATAA GAGCTCAAAA GGAAGCTGAG AAGAAAGAAA AGAAGGAAAT TACCTTTTCT 
TTTTTGTTGT ACCTCAATGC TCTTTGCATT CTTCTCTCTC CATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001328990.1
CDS	197..1804
Translation	MGVEVVNSGGFEVAPAPFEGKPEKNGKLDQGKGDDAPINFGSVGELPKNAEENNNKVVNSDAPKNAAEEWPVAKQIHSFYLVKYRSYADPKIKAKLDLADKELEKLNKARTGVLDKLRAKRAERSELFDLLDPLKSERKGFNTMFDEKRKEMEPLQQALGKLRSNDGGSARGPAICSSEEELNSMIYSYQYRIQHESIPLTEEKQILKEIRLLEGTRDKVIANAAMRAKIKESMGQKDDIQGQVKLMGAGLDGVKKERQAISARINELSEKLKATKDEITVLENELKTVSEKRDKAYSNIHDLRRQRDETNSEYYQNRTVLNKARDLAAQKNISELEALANAEVEKFISLWCSKKNFREDYEKRILQSLDSRQLSRDGRMRNPDEKPLIAPEAAPSKATPSETEVVPKAKAKPQPKEEPVSAPKPDATVAQNTEKAKDAVKVKNVADDDDDEVYGLGKPQKEEKPVDAATAKEMRKQEEIAKAKQAMERKKKLAEKAAAKAAIRAQKEAEKKEKKEITFSFLLYLNALCILLSPF

Target ORF information:

RefSeq Version	NM_001341859.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana proton pump interactor 1 (PPI1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341859.1

ORF Insert Sequence:

ATGGGTGTTG AAGTTGTAAA CTCTGGTGGA TTTGAGGTTG CTCCGGCTCC TTTCGAGGGG 
AAACCTGAGA AGAACGGGAA GCTGGACCAA GGAAAAGGAG ATGATGCCCC CATCAATTTT 
GGTTCAGTCG GTGAGCTACC AAAGAATGCC GAAGAGAATA ATAACAAAGT GGTCAACTCT 
GATGCACCCA AAAATGCAGC AGAAGAGTGG CCTGTGGCTA AGCAGATCCA CTCTTTCTAC 
CTTGTTAAAT ACCGTTCTTA CGCTGACCCC AAAATTAAAG CTAAGCTTGA TCTAGCTGAT 
AAAGAGCTTG AAAAGTTGAA CAAAGCTCGG ACTGGAGTTT TGGACAAGTT GAGAGCTAAG 
AGGGCCGAGA GATCAGAGTT ATTTGATCTG CTGGACCCGT TGAAGTCAGA GCGTAAGGGA 
TTCAACACAA TGTTTGATGA GAAAAGAAAG GAAATGGAAC CATTGCAGCA AGCACTGGGA 
AAGTTAAGGA GCAATGATGG TGGGAGTGCT CGTGGCCCTG CTATCTGTTC CTCCGAGGAA 
GAGCTGAATA GTATGATATA CAGCTATCAG TATCGGATTC AACATGAAAG CATTCCGTTA 
ACCGAGGAGA AGCAGATTCT CAAAGAGATC AGGCTGCTTG AAGGGACAAG AGATAAGGTC 
ATTGCTAATG CCGCTATGAG AGCCAAAATC AAAGAATCTA TGGGTCAGAA GGATGATATC 
CAAGGTCAAG TTAAGTTAAT GGGTGCTGGT TTGGATGGAG TGAAAAAGGA GAGGCAAGCA 
ATTTCAGCAA GGATTAATGA GCTGAGTGAG AAGTTGAAAG CTACCAAAGA TGAGATTACG 
GTACTGGAAA ATGAGCTCAA GACTGTGAGC GAAAAGAGAG ACAAAGCCTA TTCAAACATT 
CATGACCTCA GGAGGCAACG CGATGAGACG AATTCCGAGT ATTACCAAAA CCGTACTGTG 
TTGAACAAAG CTAGAGACTT GGCTGCACAA AAGAACATAA GCGAGCTTGA GGCGCTAGCC 
AATGCTGAGG TTGAGAAATT TATATCCCTT TGGTGCAGTA AGAAGAATTT CAGAGAAGAT 
TATGAGAAGA GAATCTTGCA ATCACTTGAT TCTAGGCAGC TGAGCCGAGA TGGACGGATG 
AGGAACCCCG ATGAGAAGCC TCTGATTGCT CCAGAGGCAG CACCATCAAA GGCAACGCCT 
TCTGAAACCG AGGTTGTCCC TAAAGCTAAG GCAAAGCCGC AGCCAAAGGA GGAACCAGTT 
TCTGCACCTA AACCAGATGC TACTGTTGCT CAGAATACTG AGAAAGCTAA AGATGCAGTA 
AAAGTAAAGA ATGTTGCTGA TGATGATGAT GATGAAGTAT ATGGTCTTGG AAAGCCGCAG 
AAGGAAGAAA AGCCGGTTGA TGCAGCTACG GCGAAGGAAA TGAGAAAGCA AGAGGAGATT 
GCTAAAGCCA AGCAAGCCAT GGAAAGGAAG AAGAAGCTGG CAGAGAAAGC TGCTGCTAAA 
GCTGCTATAA GAGCTCAAAA GGAAGCTGAG AAGAAAGAAA AGAAGGAAAT TACCTTTTCT 
TTTTTGTTGT ACCTCAATGC TCTTTGCATT CTTCTCTCTC CATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
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PPI1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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